LOCUS       DAJSVD010000001        64572 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSVD010000001 DAJSVD010000000
VERSION     DAJSVD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.001"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ON498_000001"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ON498_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3938307.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ON498_000002"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ON498_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3938308.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ON498_000003"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ON498_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3938309.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ON498_000004"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ON498_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3938310.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ON498_000005"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ON498_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3938311.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ON498_000006"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ON498_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3938312.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLKENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ON498_000007"
     CDS             complement(8557..9027)
                     /locus_tag="ON498_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR3938313.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ON498_000008"
     CDS             complement(9073..9498)
                     /locus_tag="ON498_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR3938314.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ON498_000009"
     CDS             complement(9565..9834)
                     /locus_tag="ON498_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR3938315.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ON498_000010"
     CDS             complement(9845..11440)
                     /locus_tag="ON498_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3938316.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ON498_000011"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ON498_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3938317.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="ON498_000012"
     CDS             complement(14993..15553)
                     /locus_tag="ON498_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3938318.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ON498_000013"
     CDS             15906..16880
                     /locus_tag="ON498_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3938319.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ON498_000014"
     CDS             17050..18450
                     /locus_tag="ON498_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR3938320.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ON498_000015"
     CDS             complement(18528..19250)
                     /locus_tag="ON498_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3938321.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ON498_000016"
     CDS             complement(19329..19835)
                     /locus_tag="ON498_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3938322.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ON498_000017"
     CDS             19958..21757
                     /locus_tag="ON498_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR3938323.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..23399)
                     /locus_tag="ON498_000018"
     CDS             complement(21825..23399)
                     /locus_tag="ON498_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3938324.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="ON498_000019"
     CDS             complement(23654..24163)
                     /gene="mreD"
                     /locus_tag="ON498_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3938325.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="ON498_000020"
     CDS             complement(24167..25021)
                     /gene="mreC"
                     /locus_tag="ON498_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3938326.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25176..25814)
                     /locus_tag="ON498_000021"
     CDS             complement(25176..25814)
                     /locus_tag="ON498_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR3938327.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25815..26465)
                     /locus_tag="ON498_000022"
     CDS             complement(25815..26465)
                     /locus_tag="ON498_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3938328.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="ON498_000023"
     CDS             complement(26478..27377)
                     /gene="rsgA"
                     /locus_tag="ON498_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR3938329.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27542..29611)
                     /gene="pknB"
                     /locus_tag="ON498_000024"
     CDS             complement(27542..29611)
                     /gene="pknB"
                     /locus_tag="ON498_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR3938330.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29608..30348)
                     /locus_tag="ON498_000025"
     CDS             complement(29608..30348)
                     /locus_tag="ON498_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR3938331.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30361..31719)
                     /gene="rsmB"
                     /locus_tag="ON498_000026"
     CDS             complement(30361..31719)
                     /gene="rsmB"
                     /locus_tag="ON498_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3938332.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31719..32657)
                     /locus_tag="ON498_000027"
     CDS             complement(31719..32657)
                     /locus_tag="ON498_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3938333.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32650..33141)
                     /gene="def"
                     /locus_tag="ON498_000028"
     CDS             complement(32650..33141)
                     /gene="def"
                     /locus_tag="ON498_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3938334.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33156..35564)
                     /gene="priA"
                     /locus_tag="ON498_000029"
     CDS             complement(33156..35564)
                     /gene="priA"
                     /locus_tag="ON498_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3938335.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35817..36122)
                     /locus_tag="ON498_000030"
     CDS             complement(35817..36122)
                     /locus_tag="ON498_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3938336.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36127..36741)
                     /gene="gmk"
                     /locus_tag="ON498_000031"
     CDS             complement(36127..36741)
                     /gene="gmk"
                     /locus_tag="ON498_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3938337.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37228..37512)
                     /locus_tag="ON498_000032"
     CDS             complement(37228..37512)
                     /locus_tag="ON498_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938338.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37530..38420)
                     /locus_tag="ON498_000033"
     CDS             complement(37530..38420)
                     /locus_tag="ON498_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3938339.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38440..38658)
                     /locus_tag="ON498_000034"
     CDS             complement(38440..38658)
                     /locus_tag="ON498_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR3938340.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38628..40328)
                     /locus_tag="ON498_000035"
     CDS             complement(38628..40328)
                     /locus_tag="ON498_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR3938341.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40595..41368
                     /locus_tag="ON498_000036"
     CDS             40595..41368
                     /locus_tag="ON498_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938342.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41449..42018)
                     /gene="thiT"
                     /locus_tag="ON498_000037"
     CDS             complement(41449..42018)
                     /gene="thiT"
                     /locus_tag="ON498_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR3938343.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(42084..42186)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42192..43163)
                     /locus_tag="ON498_000038"
     CDS             complement(42192..43163)
                     /locus_tag="ON498_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3938344.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43257..44630)
                     /gene="glmU"
                     /locus_tag="ON498_000039"
     CDS             complement(43257..44630)
                     /gene="glmU"
                     /locus_tag="ON498_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3938345.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(45133..45951)
                     /gene="purR"
                     /locus_tag="ON498_000040"
     CDS             complement(45133..45951)
                     /gene="purR"
                     /locus_tag="ON498_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR3938346.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(46118..46942)
                     /locus_tag="ON498_000041"
     CDS             complement(46118..46942)
                     /locus_tag="ON498_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3938347.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46915..47595)
                     /locus_tag="ON498_000042"
     CDS             complement(46915..47595)
                     /locus_tag="ON498_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3938348.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47618..48556)
                     /locus_tag="ON498_000043"
     CDS             complement(47618..48556)
                     /locus_tag="ON498_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3938349.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48801..49664)
                     /locus_tag="ON498_000044"
     CDS             complement(48801..49664)
                     /locus_tag="ON498_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3938350.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49806..52379)
                     /gene="mprF"
                     /locus_tag="ON498_000045"
     CDS             complement(49806..52379)
                     /gene="mprF"
                     /locus_tag="ON498_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3938351.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52520..53326
                     /gene="proC"
                     /locus_tag="ON498_000046"
     CDS             52520..53326
                     /gene="proC"
                     /locus_tag="ON498_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3938352.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(53405..54961)
                     /gene="rny"
                     /locus_tag="ON498_000047"
     CDS             complement(53405..54961)
                     /gene="rny"
                     /locus_tag="ON498_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR3938353.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(55372..56421)
                     /gene="recA"
                     /locus_tag="ON498_000048"
     CDS             complement(55372..56421)
                     /gene="recA"
                     /locus_tag="ON498_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3938354.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56530..57771)
                     /locus_tag="ON498_000049"
     CDS             complement(56530..57771)
                     /locus_tag="ON498_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR3938355.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(58011..58589)
                     /gene="pgsA"
                     /locus_tag="ON498_000050"
     CDS             complement(58011..58589)
                     /gene="pgsA"
                     /locus_tag="ON498_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3938356.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58798..59721)
                     /locus_tag="ON498_000051"
     CDS             complement(58798..59721)
                     /locus_tag="ON498_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3938357.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(60460..61746)
                     /locus_tag="ON498_000052"
     CDS             complement(60460..61746)
                     /locus_tag="ON498_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3938358.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61739..63007)
                     /locus_tag="ON498_000053"
     CDS             complement(61739..63007)
                     /locus_tag="ON498_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3938359.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            63259..63720
                     /gene="mscL"
                     /locus_tag="ON498_000054"
     CDS             63259..63720
                     /gene="mscL"
                     /locus_tag="ON498_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3938360.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63779..64225)
                     /locus_tag="ON498_000055"
     CDS             complement(63779..64225)
                     /locus_tag="ON498_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938361.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(64318..64563)
                     /locus_tag="ON498_000056"
     CDS             complement(64318..64563)
                     /locus_tag="ON498_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR3938362.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT        17830 a        14001 c        11545 g        21196 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctcttt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttgttga agattcaggc actgttgttg attcttctgt tgctgaagat
    22381 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcgctgttgt tgattcttct
    22441 gttgctgaag attcaggcgc tgttgattct tctgttgaag attcaggtgt tgttgattct
    22501 tctgttgctg aagattgtgt agctgatgat gattcttcag tcattgaaga ttgtgtagct
    22561 gatgattctt ctgttgctga gctttgtgca gtagatgaag cttgagtagc ttgtgcttca
    22621 gcctctgctt gttcgcgtgc ttctttttca gctttttctt gtgcagcttg ttgacgagct
    22681 tgttctgcta gacgttgttg ttcacgaata cgagcttgct ctgcttcagc ttctgctttt
    22741 tgacggttca agtcagcttt cttgtcttca gcagttgctt gttcagcagc taatgaagtt
    22801 ttcagtacgt tcaaatcagc ttgtttagat aaaagatcgc ctttttgaga ttccaaagca
    22861 gcttggtttt cagctaattc ttttagttta gcatcgtttt cagctttttt gtcttctaca
    22921 gcttttttat cttgtttttg ttgttccatc aagtcgttgt ttgcttttac catcgttgtc
    22981 attgcttgaa cgcgtccaat agcatctgct aatgaatcag cattcaatac agcatcgatg
    23041 tagtttgagc ttgtgttgct tacttgtgct tcgcgtgctt gtttttggat tgtatcttca
    23101 cgtttttcaa tacgttcttg taaatctgca atgtctttta ctaattgtgc agattcttga
    23161 cgcaatgtat cttgttttgc taataggtct tgtgcttgtg tgttgatttc agaaacttgg
    23221 ctttctaagg catcgatttg tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc
    23281 ttttgatctt gctgttgtat ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat
    23341 gctacggcag tcaaggtcat tgaacatacc attactgctg atattaaact ctttttcact
    23401 cttcattcct ccgactggct taatttaata aatatctaaa tatatttaag tctttcttaa
    23461 cgatataaaa attgtaccat agcagtgtgt caacgatata atagttatgt tacaaaaatg
    23521 tttcaaaaca acttttaaaa atgacaaaac tgttcgtttc agaaaatgat catgaaaaag
    23581 ccgcgttaag tctagcattt cttaagataa caacgattct ttttcgcttt cgacctaaaa
    23641 ataaaaaaag aaattattca tcactgaata atttcttaaa gggaaaaatg aataatacaa
    23701 atataatcat gttcaacagc agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat
    23761 ttactactgc taatttaaaa atcaactgga ccaccatcgt aaacaattca taacccgtga
    23821 caaagatgat catactgaaa aattcagtaa agatgttgac atggataacg tctttcatta
    23881 catacatcaa ccatacaatt aaagggagag ctactgcata gataccgata acgccaatat
    23941 agtaagcgtc atagatagcc ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa
    24001 acgccatact gcaacataat aatgccaata tcagaaagtg ggcatttgac atataggtgc
    24061 ctttcgacca ctcccctagc attcttgtta aatgaccatc tatcaacatc aaaaagaaca
    24121 aaacgattgg cagatagtac ttcattgttt ctttcttcaa catggactaa tcctccgcta
    24181 gccgttggac aatcgtcaca actgacacgt catacatttc tgcataaggt ttcacataaa
    24241 cttcccgatc cagcccataa ctatctggtt tcgttttaat aaccgtaccg atcggcaagt
    24301 tagctggaga attccctcca agaccggatg tctggacaac atccccttct ttgatatccg
    24361 tatcaccagt taattgggtc accactaagg catggagctt ttcatcatag tttttaagca
    24421 aaccaaacgc ttcgccatta gccgaagata cccgtactgg aaaatgattg gagctttcat
    24481 tagaggatga cagtaattcg attttagacg aagccgtatt gacctcgatc actcggccaa
    24541 tcaatccttt ttgcgacatg actgccatat ttgcttcgat accgtcttta gaacccttgt
    24601 ctacgatcag catgtcctgc caactgtcag gcgaacgagt aatgacattt gccgttactt
    24661 tttcgtaact agtcagcgtc tgattcaact caagttcttt tttcaacgct tcgatttctc
    24721 tctcgtaatt cttgttttgc tgtgaaacgt catcatagct atcgattttt tcttttagcc
    24781 gctcattttc ggcataagtt gaaaaaagat tatggacaga atctactcca tttcgtaccc
    24841 accgtactgg ggcagagatt gttttatcaa ccattgcaac actgtcatta acgatggatt
    24901 ggaagaaatt gggctttcct tcattcgctc gctgcgcagc ggtcacactt agtatcgtca
    24961 ccacgatgat aacaataacc aaggtaataa taatattttt attaggattg aacttcttca
    25021 caaagacacc tcgaattaat tagactcttg tgttcaattt tatcactaaa tgccgtgaaa
    25081 caaaaggtaa gttgctcctc ttaagctttt ttttagcaaa taatagattc tgtcattttt
    25141 agcctgaaag acaaattttt ttatttgttt atggatcaat ctttgctttg aataactgca
    25201 atcataccat ctgaaaaaga aaattctgcg gtatctgaaa gaaattcgtt acttgcataa
    25261 gatgtcggat attctacttc tttattttcc aatacatact tgctgccttt taatgtaaga
    25321 ttcgagacgg gagtcaagca gcaataagct aaatacttca tatctggtat ctgtcttact
    25381 atatgctctc caggcaaata ataagacaaa taattttgac aatctctgat aataagctga
    25441 tgggcaaagg gtttgaagcg aggttccata cctaaccaga gattggctaa aagatgatcc
    25501 aatcttccgc ccgtagctcc aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt
    25561 tttaagatac ctaactgtgt atctgtatcg tctttttctg caggggaacg ggtatactct
    25621 ttggcttttg caaatacttc cttctgctct tcgttcgaca atgaatcaaa atcacccact
    25681 gccagatcta ctgggtagcc tgcttgaagc aggtacagcg caccacggtc tatcccgaca
    25741 taggcatcgt atttggacag cagagactgc ggccagtttt gcggattacc tccagcagct
    25801 aataaaattt tcatttagtc taatgcctct tttaatcgtt cgatttgtcc gactggattt
    25861 tcggctccat agatatatga tcctgcaacg aagacatccg cccctgcatc tttacagatt
    25921 tttgctgttt ctggaacgat tccgccatct acttcgattg cataagtcca attgttctct
    25981 ctacgtagat cagcgagttc cgtaattttt tctactgttt cttcaataaa gctttgtccg
    26041 ccgaacccag gattgacagt catgactagt acttgatcag ccaaaggcaa aacatgtttg
    26101 atcatggaga ctggtgttcc tggattgata actactccag ctttaactcc taagcttttt
    26161 gtcatttgaa gtgcccgatg aatatgagga gttgcttcga cgtggacagt aatgatatct
    26221 gctccagctt ttgcaaatgc tggaatgtag ttttctggat caacgatcat cagatggacg
    26281 tctaacggta atttagtaac aggacggatt gcctgaacaa tatttggccc taatgtgata
    26341 tttgggacaa atcggccatc cattacatct acgtggatgt aatctgcgcc tcctttttcc
    26401 actaactcaa tgtctctttg taaattggca aaatctgcac tcaaaatgga tggtgctatc
    26461 ttcattcttg tttcctccta ttttcgttta ccttcttttt ttgcatacat tggttttctt
    26521 ttttcgattt cttgtaagaa ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct
    26581 gtttctactc tatgttttac ttcgcagcca ggttctttag catgcatgca ctctctaaat
    26641 ttgcaatgga cagaagcttc tacaaattca ggaaattgtc tcggcagttc tcctgcttca
    26701 attgtcaaaa agtcaatcga gctaaatcct ggtgtatctg ctactaaccc gccatagata
    26761 ggaatcagtt ccacatgacg tgttgtatgc cgtcctcttc caagtgcatc agaaatctct
    26821 cctgttgcta aattcagttc cggtgatatt tgattcagta gcgtcgattt tcctgctcca
    26881 gactgaccca taaagaccga tagccgttct gggaagtaac ggatcagttc tgtcgtatca
    26941 cccgtatgat tgggtacaat caccggatag cctattttag tataggtttg cttgatctct
    27001 tcgatttttt ctgggtcgtc tgacaagtcg gtctttgtca aaaaaataat cggttcgatc
    27061 gactcgtatt ccaaggtaac tagaaagcgg tcaagtagat tataagaaaa tgcaggctct
    27121 acgatacttg tgacaatgat tccaagatca acatttgtga ctggtgggcg aactaattga
    27181 ttttttctcg gcaagatttc cagaagatag ccgtctgttt gattttcact ctcaaagatc
    27241 acctgatctc cgacaagcgg cgtgattttc cgattccgga aatttcctct tcctcttgtt
    27301 tgataggtct ctccgtttgc gtatacatag taaaaaccgc tgattgcttt tctgatttgt
    27361 cctttgataa gtgccacctc cgtttttgtt tattttacca tacgaagagg aaagtaggtg
    27421 ttattccatg caaacttctc aaagtatatg gcttgaaaat tgatcaaaag agatagaaac
    27481 acaaaaaaga gaatgcaaca aatcaatgtc gcattccctt ctgatatatt ttattcagca
    27541 cttattttga actttctgag gtgctagctg atgaacttgt ggagctggaa gattcttctt
    27601 ttgaagagtt gcttgtgcta ctggagctac ttgagctgct agaactgtct gaagagtctt
    27661 tttcagaact agaagacgct ggatctggtc ctttagagta aattacgttc acaaccattc
    27721 ctttggtaat tgtcgttcct gcccctggta ctgtacgaat cactttatct ttatccaccg
    27781 tgtttgaata ttcggaagtc tcatgcccga tatattctgc accgacacta gctaaataac
    27841 tttgtgcttc gctttttgta tagccggaga tatcattcaa tttaggctct gttccttggc
    27901 ttacgacaaa attgatgtaa gtttcttctg ctacaacttc acccgaggct ggatcctgac
    27961 tgatgatcga acctgatggt acagtgtcgc tatagtcata agtagcacta atattattga
    28021 tattgaatcc ttgtgtaaca agccaattac gaacagtttc gtagttttct cctacatgac
    28081 tactcaaagt cactgtttct ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag
    28141 gatcgacttc tgtatcttct gatggagatt gcgaaatgat cattccgctt tctacctctg
    28201 aactgaattc ctcttctttt tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg
    28261 cttcggcatc ttcataactt ttaccactgt aatcatctaa tttgattttt ttcgtgccgc
    28321 tactaatata cagagtgact tcgcgttttt gcttgactgt cgttcccgct gccgggtctg
    28381 tcttcactac attaccttct tcgattttgt catctgcaat ttcttctgtt tttgttgcta
    28441 cttctaattt cgcttttgcg agtgtttctc tagcagaagc ttcagaaaga tcagaaacat
    28501 caggaacaac aacttctcct cgcccgccag aagcaaagaa tacagctgtt ccgattccta
    28561 aaacagcaag aatagctaat aggatcagcc accatttttt ctgtcctttc tttttaggat
    28621 caggcagaac ttcttcctcc tctggtaagg tttcgttttc tggcttgtct tttggcagag
    28681 gcatagattt gaatgaatca ggcattggtg catcttccgt aaggggtgtg ataacttttg
    28741 tttcattatc catcacatga ggctgccatt tttcttcgtt tgcacgagct ggtgataata
    28801 ctgtagaaag atcatcagac atttcctcgg ctgacttata acggtctgca ggttctttag
    28861 ccgttgcccg taaaatcacg ttttccagtg cttgtggcac atttggatca agtgctttta
    28921 tagatggaag atcatcttga aaatgtttta aagcaattgt tacagcagat tcaccatcaa
    28981 acggtacgct acccgtcaac atctcataaa ggatgatccc taaagcataa atatctgact
    29041 gcttcgttgc catacttcct ctggcctgct caggggacag ataatgaaca gatcctagca
    29101 tcgtattcgt ctgcgtgatc gatgtttcag ataaagcgat agcaatcccg aaatccgtga
    29161 tttttacaac accttcatta tcgatcagta cgttttgggg tttcaaatct cggtggatga
    29221 tttgatggtt atgagctaaa gaaatagcag aaaggatctg ctgcataata tttactgctg
    29281 tttcataagg aacaggataa tgtgtttgga tatatcgttt caagtccatt cctttgacgt
    29341 attccatcac cagatactgc atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt
    29401 ttggatgaac gagttctgtt gctgctaaag cttctctctg aaatcgtcga atcgcagtct
    29461 ggtcattttg aaagtcaaat cgtaagactt taactgctac atcacgatcc aagattaaat
    29521 cgtgcgccaa aaatacattt gccataccac cgctaccgat atttccagtg atttgataac
    29581 ggccatttaa ttttttgccc aactcgatca tacactgtcc tccttatatt cgataagaag
    29641 aacggtgata ttatccgctc cgcctgcttc attggcacga tcaatcagct cagttacttt
    29701 atgagacaat gggccttctt gattgatgat cgttcctatt tcttcttcag acagcatgtt
    29761 cgtcaagcca tctgaacata acagcagtcg atcttttagt tgccaaagat gagtagaaac
    29821 atctacttcg actgttccag gcatgccgac agaacgtgtt aaaatatttt tccttggatg
    29881 gttcactgcc atttcttcgc tgatttctcc tgatttgact agttcattga ctaaagaatg
    29941 atcctccgtc aattgcatga ttttatcttc acgaatcaaa taagctctgc tatccccaac
    30001 attggcaatc gtgaaccagt catctgacag agcggccgct acgattgttg ttcccatacc
    30061 gttatattcc ggctgttctt gtccacgctg ataaatcagc atattttctt cttgaatcga
    30121 ttgaatgaac cattgggcga ttttttcatc gtcaacagct tcttgttttt cccaagcttc
    30181 gcctagattc gttaccgcca tctgactggc aatgtctccg gcacgatgac cacccatccc
    30241 gtctgctaat acagcaagct tgagacctga acggctggta aatacactgg catagtcttg
    30301 atttgtgtta cgtcgtcttc ctacatctga ttgatattca atctgcattt tttcacctca
    30361 ttactttttg cgcaaacaac aaatgaaaaa tccatctgtg taaaactgat gcggatacaa
    30421 agtcaacatt ttctcttcga ttgatgattg tagcatagga ttggtcaaaa catcaatcct
    30481 ctcaaaatca gggtgtcttt ccaagaaagc agaaacaacg tcttggtttt cttcttttaa
    30541 gatcgtacaa gtgctataag tcagaatacc ggaagacttt aaagttggtg cgcagctttc
    30601 taaaatttcc aattggattt ttggtaaatt cgctaattca tccggctgtt ttttataacg
    30661 aatatctggt tttcgccgaa gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg
    30721 atcaaactgc tctgcttcga actgtttact gacttctcta gcatccatct tttgagcgta
    30781 gacagcagaa gagacaccta gacgtgctgc attatcttca atgagtttga ctttgtgttc
    30841 gtgtacatct aaagcgacca cttctccacc gcatgacgga tctaaaaaag aagcaatatg
    30901 cgtcgtctta cctccgggtg ctgcacaggc atctaagaca tgatgttcag gctcgatttg
    30961 catagacggc gctacaagca tcgaactttc atcttggatc gtcatgacgc catcgtggaa
    31021 caatgagcta gaagctaaat gccctttttt cgctactata ccgtatggac tgacttggct
    31081 tttttcagct gtgatctctt cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg
    31141 gcgtgtatct actcgggcac tagcatggct aggctgatat agcgataaac cgagcttttc
    31201 agtttcttcg tatccgattt gatccaccag tcgtttcgtc atccatagag gcaggctgat
    31261 tttgacagaa agtctttcga taggatcagc aatgccttgc agatctggtg ctccttgacg
    31321 ctgataattc ctcaagacac catttacaaa tttccctgtc ccaggatttc cttttacttt
    31381 tgcgatttcg accgcctcgt ttaaaatagc atgtgcaggg actttatcta aaaattctaa
    31441 ttggtataca gaaagataaa gaagcatctt gacccagtca tccacttttt ttgctttagc
    31501 gatcaatggt gcaacgtaaa attccaatag tcgctgatgg ctaatcgtac catagatcaa
    31561 ttctgtaaat aaacccacat ctttttgatt tagctcgctt tgtttgatca tctcattcaa
    31621 caaaagattc gagtaggcgc cacccttgtt cactcgctct aatgtcagta atgccatata
    31681 tcgtactgat tttttcattt ttttagggat ttttttcatt atttcacctg ctgaccaatc
    31741 tgaacttgct gaccgcttcc gttaaggaaa tcatgtacgg cctgttttcc tttaccagct
    31801 ggctgtaatt cattgatggc taagacggtc tgtttaccgc aagcaatcca tagatttttt
    31861 ttgtctttcc ggatgatcgt accaggtgct ttgtctgtag tttctgccaa tacttcaacc
    31921 gataataatt tccagcgtat gccctcatag gtcgtgaatg caattggcca aggacgcatt
    31981 ccgcgtactt ggttgtcaat ttcttctgca gttttatgcc aatcgattgc ttcttgttcc
    32041 cgtgtgatgt ttggcgaaaa agttgcttct gactcgtctt gaggtactgg tttttgtcca
    32101 tccaaaatat tgggcaatgt ttctagcaat agctttcttc ctaaaaggct aagcttttca
    32161 aacatcgtcc ctacatcatc ttgtttagtg attggtatgc tttcttgcgc atagattccg
    32221 cctgcatcca tttttttgat catttccatg atcgttacac cagtttcttt ctccccattc
    32281 atgatggcat aatgaacagg cgcacctccc cgatatttag gaagtaaaga agcatggaca
    32341 ttgattgccc ctaattttgg tacttgcaat aacttttcag gcaagaattg gccaaatgcc
    32401 gcagtgacga tcacatcagg caccaattca atgatttctt ccatttctgg cgaaccggag
    32461 attttctctg gctgcagcac acggataccg tgcttcagag ccgctttttt taccggtgtc
    32521 ggcgtaatta ctcgtttccg cccaactgga cggtcgggct gtgttaccac tgcctggatc
    32581 tcgtaacctg attctatcag cccttcaaga atcggcacag aaaaagcagg tgtgcccata
    32641 aatactagct tagtcatcaa gatgttcctc catatattct tcaagttctt cttccgggat
    32701 ctgctgtatc attttctcaa tgaacaagat accgtctaag tgatcgattt catgctggaa
    32761 ggcacgggct aaatagccaa aagccgtcac ttctatctct tctccgtctc gatcataata
    32821 acgcacggtc acttcatctg ctcgcttgac cgttccatag acatgcggaa tactgagaca
    32881 accttctacg tcaaggcttt caccttttgc ttcgatgatt tctgggttaa tcagttcaaa
    32941 tttctcgcct tcatctactt cgatcacagc aattctcttg ttttgtccta cttgaggagc
    33001 agcgatccct attccgtcat tcgcaatcat tgtctcatat aaattgtcta ataaagcgat
    33061 tgtctcgtcc gtgattacat cgattggctg agcagttctt tttaatttat cattagggtg
    33121 gatcaatata ggatagcgca taatttggcc ctttcttaaa taaagtttaa tggttcgttg
    33181 tcaattgcca caaaaagacc ttgtcgctgt tctttctggc ttccattcaa aatttcatcc
    33241 aaaacatgag gaagattcgg ttcatgttta tatttgatga ttagttgata ataatatcgg
    33301 tttttcactc tcaaaatcat gctgggcgtt ggccctaata gcaagctttg agggcttaac
    33361 acttctttta gctgattggc aatctgaaag attttctttg ccgctacttg ttcttcagga
    33421 tggctggcgg ttattttcac agtaaagtaa taaggaggat agcctccacg atgccgtaca
    33481 tgcatttcct gctgataaaa ctgctcataa ttttgttttt tagccaattc tatggcgtaa
    33541 tgttgaggat tgaatgtttg gatcacgact tcgcctgctt tttccgctcg ccctgctcgt
    33601 cctgaaactt gggtaagcaa ttggaatgta tgctcactag aacgaaaatc cggtaaattc
    33661 agggaggtat cagcattcaa caccccaact aatgtcactt ctggaaagtc caaaccttta
    33721 gcaatcattt gtgtccctaa taatatatct gcttcttttg caccgaattt ttgtaaaatc
    33781 tgttcgtgtg ccccttttct tcgagtggta tctacatcca tacgcaaaat cctagcttgc
    33841 ggataaagtt ctcggagttc ctcttctact ttttgagtac ctgttccata gtagcggatt
    33901 ttattgccgc cacaattagg acagcgattg ggaataggtt cttcatgtcc gcaataatgg
    33961 cagcgcattg aacgtgtatc catatgcagt gtaagagaga tatcgcaatt aggacatgga
    34021 agaacaaatc cacagtccct acacatcaca aaagacgaat atcctcgacg attgagcagt
    34081 aggacagttt gttctttttt gtctaatcga ttttgtattt tttcctgcaa attagcagaa
    34141 aaagtagagg tccgatgatt ctgaaactct tcacgcatat ctacgatctc aatagctgga
    34201 agctgtgcag ctgctttggc tcggtggttt aactgcaata actgataaac gcctttttga
    34261 gctcgtgctc ttgattctaa agagggcgtc gcgcttccta gaactattgg gcaatgatga
    34321 tatttactgc gccaaattgc tagatctctc gcatgataac gcggtgtctc atcttgtttg
    34381 taacttgatt catgttcttc atcgataatg atcagcccga tatttttcag cggggcaaaa
    34441 atagcagatc gtgcgcctac tactacttgc gcttcttccc gctcaatctt gcgccattca
    34501 tcatattttt caccttggga aagtccgcta tgtaagaccg caactgattc tccgaagcgt
    34561 cctttgaatc gctcgaccat ttgcggagtc aatgcgattt caggcactaa cataatagcc
    34621 gttttatttt ctgacaaaac atcagcgatt gcctgaagat atacttctgt tttcccgctg
    34681 ccagtgattc cctccagcaa aaagacctga tctttttgct gctgacctgc tgtgatgatt
    34741 tgttcgacag catttttttg ttctgcattc agttccaatg ccgtcgtttg gtcaaatact
    34801 cggtctttat aagggtcgcg ataacgttct gattctataa actccagcca gcccttttct
    34861 tttccttgat tcaagacagc agtactgatt cctttatttt tgaaataagc aattggttct
    34921 tcttggctca gttcagataa acacaaaaga agttcttttt gtttcttggc attttgccgt
    34981 aattttgccc attcttcttc gatttgcttt tcagttaaca aagaacggac caaacgaatc
    35041 gtttttactt tatttcgtgt atggacttca tatttgatat ctactaattg ctcttggcgc
    35101 cagcgaatca attgagaaag gctgccttct tcttctgctt ccttccagga aatcacttct
    35161 cgctgatcaa acaatacctg aacttctggt gtgtctgcca aaggatagat caatttatca
    35221 tattctgctt tcatcacact cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa
    35281 gtaatttttt tcatataatc agctaatgaa agtaattctt cattgaccac cggagcaagg
    35341 tctagcaaac gaataactgc cttcaaagat ggattagctg attcttctga tttttggata
    35401 gccataacaa atccttggac atgacgattg ccattaccaa aagggacttc tactcgcatc
    35461 ccgacttgca aagctgtttc catttcagag ggaaccaaat aagtgaatgg ctgatcggtc
    35521 tgcatagtag gtacgtccac aatgacttca gctgttaatg tcatggttcg tttcctcctt
    35581 tttttgtctt ttcttatcaa gcgacaaaaa gttccttgcc tattttacta gagatcgatg
    35641 gatttgtctt atcttctgcc aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag
    35701 aactgtgtca aaaagttcag acacagcctt ttctaccaga caataagtgg cgaatgtttg
    35761 aaaaggaaac agctgggttt gcttttcttt ttagatgttc gctctatttt tctttattac
    35821 attttttgct cttcacggat acgtgattcc aattcgtgtt gttcacgctc tttttgtgct
    35881 tgacgttcct cttcttccat cttcaaacgt tcacgtttta attcaggatg tggatcattg
    35941 actacatttc ctgcatcgat ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa
    36001 gaatctagag taggctgtgc acttgcatcc aattcatgcg cgcgtttact tgctaaaatc
    36061 actagagaat attttgaatt tacacgatct aatagtgagt caattgaagg ttttaacatc
    36121 ataagactac atctccttta acattttaat atatttaccg attacgcgat ctactcgaaa
    36181 atgttcactg gcaataatgt ttttgattcg ttcaactgct agcggcactt catcatttac
    36241 aacagcatag tcgtaaagag ccatcatttc gatttcttct tttgcaactt tcattcgttt
    36301 ttcgattact tcatctgcat ctgttcctcg gcctacgatc cgtgatttta attccgctaa
    36361 atctggcgga gtcaaaaaga tgaatacacc atcgggcact ttttctttga cttgttttgc
    36421 tccttggact tcaatctcca agaaaacatc ttttccttca tccaatgtct tgttgacata
    36481 tgacaaagga gttccataat aattcccgac atattctgcg tattccagca tctctccagc
    36541 ttcgatcatt gattcaaact cttctttgct tcggaagaaa taatcaacgc cttcgacctc
    36601 cccttcacgc attttacggg tagtcattga gatcgaatac tgaaaatcat tatcctcact
    36661 ctcaaaaatt gcttttcgta ctgtgccttt tcctacaccg gatggaccag acaacacgat
    36721 taataatcca cgctctgaca tgacgacgtc ctttcaacta gtctttttgt acttatcgtt
    36781 tattttgcac tttttttctg caactttcaa ggttatcttt aattatagct agaaattggt
    36841 aaaaaagcta ccctgtacca agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa
    36901 cagccaaaca taaacagctt ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc
    36961 aagcttttga acgccgtgta taagaggtcg tgaagaagcc gattagtttc gagtatgaag
    37021 aaaaattttg tgaaaatagt ttctcacatt tttgcataaa tttggcttaa taccgagaaa
    37081 atggtttttg aacatcactt attcaaaata aaaaaagatt gtgacacgaa ttttgtcaca
    37141 acctttaaat gtattcctag agcaaagaag ctaagcgaag aaatccaatt ctgtttattt
    37201 attgtatttc tttgttcagc atccttgcta aaagcccata ttggacaaag tattcactac
    37261 acccatgatc tcatcttttc gagaacgtgc tttttctgtc ggatcttcta cctcttctgt
    37321 gtcaattcga acaataaatt tccgattcat cgtcacgtaa gcaccgtcgt tgatcaaaat
    37381 aaacagttcg ttctcattat agtctgcaca ggcatctttc catacgtcaa agccgttttt
    37441 ttcagaaggg atatcccgtt taaccacagt ttttccactt aattcgtgga acgttacgtc
    37501 ataatatttc attatctcta cctctttcgt cattcaatat tttgtacttg ttctctgatt
    37561 ttttctaaga tcgtcttaga ctggacgact tgatttttaa tttcgattgc atttgatttg
    37621 gaaccgatcg tgttgatttc ccgattcatt tcttggatca ggaaatctaa ttcccgacct
    37681 acaggacatt gtgcttcgat aagttcatct agtttttgta catggatcgc cagacgatcc
    37741 agttcctcgt gaatatctcc gcgttccaac aagatggcta gctccgtcaa caaccgttgc
    37801 tcatcgactt tcgctccaag aaattcctcc agcttttttt gataccttat ttgaaattcc
    37861 ttttcataga tttctacaaa actatcgagt ttttttagta cttctttgaa ttcttcagta
    37921 ttctttttca acactaaagc taaagcttga ccttcttttt ctcgactcgc ctcaatagaa
    37981 tcaagtgcgc ttttcaatgt atctagagct aactgtgcca attcttccat gctctcttcc
    38041 ggtttttctt ctaccgttac gaagttttcg tccagcacga agcgttccgc catccgacta
    38101 attgacactt gattttgact accgtatcgt tgttgaattc cgtctgtcaa ttgagtgata
    38161 agtgaatcaa tcaattccca gttgatgacg atctgttttt cgtttttgcc aatataagct
    38221 agattcacaa atacttcgat tcgaccgcgg cttaactgct gtttaataag ctgacgaaaa
    38281 tcgttttcaa caaagttcag gattttagga cttcgtattt gaacatctag aaaacgctgg
    38341 ttgacacttt ttatttcaat atctaactga tacgcttttg tttcgcggat ggctttgcca
    38401 aaaccagtca tacttttcat tcgacagggt ccttcctcat tacatatttt ctattgcttt
    38461 ttgcagttgt tcaaactctt cgttcgtcaa tgtcaggcct ttccccattt tttcatggtc
    38521 tggcgcccaa tctcgaagat cgaattttgg cgggcgacca ttccagctga ctaggttcag
    38581 ttctttacgc caccccttgt tgttttctga caatatagcg atttcttcta cgatttcata
    38641 tgaaaattct tgtgccatgg ttgttcctcc ttgagataat ataaatatag gatcagtaat
    38701 gtgtcaactg gttgcttaag caattcatgc tgcaaacgat ccttgcgttt tccttgtagc
    38761 agataatcaa aataaacagt tttcgtgcgc tcattttgta ttttttgctg gtattttcga
    38821 taccttcgcc attcttcagt acttaattga ccaatttgtg ccaataaatc atccggcata
    38881 ctttttatat cttgttggca gatcatttga atcatcatgt ctacttgata gatttcattg
    38941 ggcagtaaaa aatgtttttt tgctaaaagt tctagcatcg ccaatccgca tgtctctggt
    39001 acgaccctgt tcttatataa attacgcagt ttttgccaaa attttatgct tttgaaaaaa
    39061 gataattcta actgaatttc ttttgttaag taagacaaaa atcctcgcca gtatgttctt
    39121 gcaggtttaa cagaatgaaa tgtataccag ttcccatagt aatagcctaa tcgttttttc
    39181 atttccagat agcctaattg taaatttaat tggttccttt ggctatcaaa aagcagaaag
    39241 ctgcctagtg tccacaatgt ttgacatttc caatggataa aaatgtctgg catacgaatt
    39301 tttttcgctg ggcctggccc ttgcacatct acgacaaatg cctctgttgc tcctttgtta
    39361 atcgcaatat caataggtat tttgttgcga tacccaccgt ctgcatattt attttttccg
    39421 atctttcgat aagccatagc gggataaaat gaagcagagg cgagtatcca atctggtatt
    39481 tcttctgttt ttgtctgttg gatatcagtc actacttccg ctagttctgg cagtttagta
    39541 gacaccgtat ataaacgtat tttgggatta tgtttgagta aggacaaatc caaattgtct
    39601 ttgaccatct tttccaatgg agcagaagaa accccgcctt caattagtgc tgttttcgtc
    39661 atttggcgtc tttctcgata gaagcgttca cgaaggtctt caactgcagc catttctggt
    39721 aacgcaagca cttgtcttgt cgacagcttt ttccataaat tcacagcttt tttcatatct
    39781 ccttgtaaaa tcaatgcgcc gttcaacgca cctaccgagg tacctgtgat aatctcaaag
    39841 ttgatatcat gttccttcag cgcctgccac acaccaatct gataagcacc gtgcgctcct
    39901 cctccaccta aaacaagtgc tgtatgatag accagctttt tatttaattg tgtggttcct
    39961 tgatagccgt ttttatgaaa aatacgcaac cattcttctg atagaggaga ttgaaataaa
    40021 aaacaacagt tttctttaat aaaaaaacga gcgcaagctt ccatataccg aaaaaatttt
    40081 ttccattcaa aagtttcacc ttcaatcaac agattattaa aaataacatc tcctctactt
    40141 ttagcaatag taaaatagac ggtatatttt ttcgatactg ctttaaaagc acattgcttt
    40201 tttgcagaac gccaaaaagc gtctctactt tcttgatgac tttgaaaaaa tggaaggggt
    40261 tgttttgctt gctggatagg cgaatacagc gacattttct gtgaaaagta aacttttttt
    40321 atctccatcg cgctctcctt tttcttcaag tataccagta cgtcataaaa aggtcatgag
    40381 tctcgtattc gactttgtag attccataag aaaacaattc ataaaacctc ctttaaataa
    40441 aaaaatacaa aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat
    40501 tttgtaatgg aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata
    40561 aaagagatta agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat
    40621 tgataccaca agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag
    40681 tatcgtgcat tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata
    40741 tgaatcccat acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc
    40801 ctcttctcgt aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac
    40861 gatgctgaaa gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa
    40921 aacacatctt cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct
    40981 taatgatcag ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc
    41041 tagtaaatta agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa
    41101 agaaacacct gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca
    41161 ggaagggatc attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc
    41221 aatctatgtg attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga
    41281 aatcatcgct actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc
    41341 cgatctttct tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac
    41401 aagaaactag gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg
    41461 tcgtttatct ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat
    41521 cgttacaata gctgtactca acccgctggc accattcatt acgaaagaaa agagccaagg
    41581 attcatcccc catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa
    41641 atatctggaa agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt
    41701 attatcgtct acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa
    41761 agtataggca atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc
    41821 caacggaaaa tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct
    41881 tcttaacgca aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat
    41941 ctggatcgga atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca
    42001 aactctattt tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga
    42061 agcagctttc gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg
    42121 atacaggtac gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt
    42181 gagtttattt attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg
    42241 atacgtttga ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt
    42301 ttacgatctt ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct
    42361 tctattcttt gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta
    42421 gttgcaccag cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc
    42481 atatcatcga tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct
    42541 gcaacatttg cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc
    42601 gctaatttac gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg
    42661 cctttgattc ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg
    42721 actggaatat caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt
    42781 gttgcaccag ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca
    42841 cgtgctttac ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt
    42901 gcacttgctc gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact
    42961 gggctgctcg ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt
    43021 acttgaattt ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact
    43081 gctgctgcaa ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt
    43141 gggtcaaagt aatgttttga catggggacc tccactttct ttttcacaac tctagaattc
    43201 tacctaataa atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag
    43261 tttagataag ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt
    43321 gccagcgcat attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt
    43381 ttagctacct cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg
    43441 acagtcgtat gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca
    43501 ttgatttctt ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct
    43561 ttgccgattt gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt
    43621 tcagcttttg gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg
    43681 ctttcaatga ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt
    43741 gaatgtgacc cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct
    43801 gcttcgatca atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa
    43861 tcgacaaagc ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca
    43921 tttgctttag ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt
    43981 tgataagcag caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag
    44041 tattcacctt gagcattgtt tgttccaact ttgcttaacg catcgaacaa agcttcgttg
    44101 tcaaaacagt aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct
    44161 ttttgttcca cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct
    44221 gttgggtttt ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat
    44281 tcaaaaagat tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac
    44341 gttgttcctt tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc
    44401 aattgttctg cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct
    44461 gctccgtgtc caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg
    44521 atatgttcta ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc
    44581 atgcgggtac ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact
    44641 cctaaatata gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa
    44701 gtgattttct tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct
    44761 atctatttgg taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata
    44821 aaacatgaaa agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt
    44881 acaacctctt cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg
    44941 caaaatatga ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt
    45001 tcacaacctc tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
    45061 gaaaaaatgt ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt
    45121 tttcacaacc tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg
    45181 agtatctaca tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt
    45241 gaattttgat tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa
    45301 gctcttcatt ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg
    45361 tgaaccacgt ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc
    45421 agacacatag ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat
    45481 aacgaatggc gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc
    45541 tacagtcatt acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac
    45601 ttgacgcagc agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag
    45661 cagacggtct tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc
    45721 atatgaaatt tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt
    45781 tccataccct cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga
    45841 tttagcagat tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt
    45901 caataaatac tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa
    45961 ctccttttaa gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca
    46021 ttttatgaaa aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa
    46081 aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg
    46141 caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga
    46201 ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta
    46261 gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac
    46321 cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca
    46381 tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta
    46441 gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata
    46501 aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg
    46561 taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc
    46621 tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga
    46681 tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa
    46741 cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg
    46801 ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca
    46861 cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga
    46921 attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc
    46981 cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac
    47041 atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt
    47101 tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat
    47161 tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc
    47221 tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca
    47281 gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc
    47341 attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt
    47401 tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac
    47461 caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac
    47521 atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat
    47581 ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag
    47641 ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag
    47701 tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct
    47761 cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca
    47821 caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg
    47881 aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag
    47941 tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt
    48001 caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt
    48061 tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc
    48121 aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt
    48181 ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt
    48241 tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca
    48301 cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg
    48361 gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg
    48421 taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt
    48481 ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca
    48541 aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat
    48601 taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg
    48661 atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag
    48721 ccatttcact acaataaaaa actgaagaat accattttag gattcctcag tttttcatca
    48781 cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa
    48841 ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg
    48901 gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg
    48961 atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt
    49021 ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga
    49081 tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt
    49141 ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct
    49201 ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc
    49261 aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct
    49321 cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc
    49381 ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag caataaagca
    49441 gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg
    49501 attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc
    49561 accatttcca actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt
    49621 atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc
    49681 gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt
    49741 ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt
    49801 gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt
    49861 cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca
    49921 aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag
    49981 gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa
    50041 aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc
    50101 attttctcgt atacaataaa cagattggct aacagatagt cagctaattc ctcagattct
    50161 ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg
    50221 atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga taatacatat
    50281 gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt
    50341 gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg
    50401 tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct
    50461 gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga
    50521 agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct
    50581 gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct
    50641 cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg
    50701 tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg
    50761 cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc
    50821 gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa
    50881 ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt
    50941 attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa
    51001 aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga
    51061 taaaattctt ttctggataa ccacacagct aacagaatca agacatatac caatatcaac
    51121 tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta
    51181 ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag
    51241 ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga
    51301 aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc
    51361 gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga
    51421 atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc
    51481 gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca
    51541 tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt
    51601 ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc
    51661 gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct
    51721 cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg
    51781 aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta
    51841 ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca
    51901 tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt
    51961 gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga
    52021 cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca
    52081 gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc
    52141 gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc
    52201 aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca
    52261 ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt
    52321 tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca
    52381 ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt
    52441 aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa
    52501 tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc
    52561 tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag
    52621 tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag
    52681 taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat
    52741 tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc
    52801 ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt
    52861 acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa
    52921 tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag
    52981 cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc
    53041 cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc
    53101 caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct
    53161 ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac
    53221 tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat
    53281 caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt
    53341 tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt
    53401 tttattattt agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc
    53461 caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt
    53521 ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg
    53581 cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac
    53641 ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg
    53701 ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga
    53761 tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa
    53821 cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac
    53881 gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa
    53941 cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga
    54001 tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc
    54061 cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac
    54121 gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc
    54181 ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat
    54241 cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac
    54301 ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg
    54361 ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt
    54421 tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag
    54481 tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt
    54541 ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct
    54601 gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt
    54661 catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt
    54721 ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct
    54781 tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga
    54841 ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca
    54901 taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca
    54961 taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa
    55021 ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact
    55081 ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta
    55141 cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt
    55201 taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta
    55261 tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg
    55321 tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc
    55381 tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat
    55441 accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat
    55501 atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc
    55561 accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga
    55621 gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt
    55681 ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc
    55741 tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt
    55801 gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc
    55861 gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg
    55921 tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa
    55981 agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc
    56041 gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac
    56101 acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt
    56161 tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact
    56221 ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac
    56281 atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa
    56341 tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc
    56401 tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat
    56461 ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat
    56521 tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc
    56581 attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc
    56641 tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc
    56701 agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc
    56761 catctgctcc acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat
    56821 ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc
    56881 agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc
    56941 tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt
    57001 atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc
    57061 taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac
    57121 ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt
    57181 tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag
    57241 tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg
    57301 tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa
    57361 agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga
    57421 ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt
    57481 tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt
    57541 tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt
    57601 ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata
    57661 ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac
    57721 gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg
    57781 ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag
    57841 ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa
    57901 cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct
    57961 tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac ttacattgat
    58021 cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc
    58081 agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta
    58141 ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa
    58201 gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat
    58261 tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg
    58321 ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta
    58381 gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa
    58441 gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat
    58501 tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta
    58561 agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt
    58621 gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga
    58681 gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt
    58741 cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca
    58801 ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt
    58861 aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat
    58921 ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt
    58981 cccttgataa acataggcat tgtctactag cacacctaac catacacgat tgacagcagt
    59041 aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga
    59101 atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac
    59161 tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc
    59221 ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca
    59281 agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg
    59341 aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt
    59401 acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc
    59461 aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa
    59521 tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa
    59581 ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag
    59641 gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt
    59701 ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg
    59761 acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc
    59821 ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg
    59881 actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc
    59941 ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta
    60001 atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc
    60061 atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt
    60121 tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag
    60181 tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt
    60241 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat
    60301 tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt
    60361 ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc
    60421 taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa
    60481 ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg
    60541 gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt
    60601 aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt
    60661 cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat
    60721 gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc
    60781 aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg
    60841 attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt
    60901 gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt
    60961 aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat
    61021 ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc
    61081 ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa
    61141 taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt
    61201 gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata
    61261 caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat
    61321 ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg
    61381 tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata
    61441 gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg
    61501 aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc
    61561 tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata
    61621 attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt
    61681 caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt
    61741 attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta
    61801 cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata
    61861 attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt
    61921 ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg
    61981 ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc
    62041 ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac
    62101 tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag
    62161 gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat
    62221 acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc
    62281 gttccttcac gatattcact tgaggttggg tataaaacag atctggataa actgtttccc
    62341 gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga
    62401 caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac
    62461 tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc
    62521 tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca
    62581 aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact
    62641 gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa
    62701 aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat
    62761 gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt
    62821 gcgcacttaa ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga
    62881 gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc
    62941 ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat
    63001 acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca
    63061 acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt
    63121 tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga
    63181 cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa
    63241 aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc
    63301 ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg
    63361 gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct
    63421 gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg
    63481 tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta
    63541 gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg
    63601 gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa
    63661 gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa
    63721 taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt
    63781 aacttgctac ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat
    63841 ctgagattgc cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga
    63901 tcacgattgg tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct
    63961 ttcttcctaa accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac
    64021 tctccgcttc ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa
    64081 acgtatgcca agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca
    64141 caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt
    64201 caaatttctt tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat
    64261 accgattcgc acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta
    64321 aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta
    64381 agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata
    64441 ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa
    64501 catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg
    64561 catacagttc ac
//
LOCUS       DAJSVD010000002        63523 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSVD010000002 DAJSVD010000000
VERSION     DAJSVD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.002"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(200..598)
                     /locus_tag="ON498_000057"
     CDS             complement(200..598)
                     /locus_tag="ON498_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3938363.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(595..1366)
                     /locus_tag="ON498_000058"
                     /pseudo
     CDS             complement(595..1366)
                     /locus_tag="ON498_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            complement(1363..2046)
                     /locus_tag="ON498_000059"
     CDS             complement(1363..2046)
                     /locus_tag="ON498_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3938364.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2213..3100)
                     /locus_tag="ON498_000060"
     CDS             complement(2213..3100)
                     /locus_tag="ON498_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3938365.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3097..3966)
                     /locus_tag="ON498_000061"
     CDS             complement(3097..3966)
                     /locus_tag="ON498_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3938366.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4103..5389)
                     /locus_tag="ON498_000062"
     CDS             complement(4103..5389)
                     /locus_tag="ON498_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3938367.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5662..6240
                     /locus_tag="ON498_000063"
     CDS             5662..6240
                     /locus_tag="ON498_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3938368.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6240..7169
                     /locus_tag="ON498_000064"
     CDS             6240..7169
                     /locus_tag="ON498_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3938369.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7166..8902
                     /locus_tag="ON498_000065"
     CDS             7166..8902
                     /locus_tag="ON498_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3938370.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8965..9801)
                     /gene="pheA"
                     /locus_tag="ON498_000066"
     CDS             complement(8965..9801)
                     /gene="pheA"
                     /locus_tag="ON498_000066"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR3938371.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9798..10307)
                     /locus_tag="ON498_000067"
     CDS             complement(9798..10307)
                     /locus_tag="ON498_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR3938372.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10300..11595)
                     /gene="aroA"
                     /locus_tag="ON498_000068"
     CDS             complement(10300..11595)
                     /gene="aroA"
                     /locus_tag="ON498_000068"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3938373.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11608..12699)
                     /locus_tag="ON498_000069"
     CDS             complement(11608..12699)
                     /locus_tag="ON498_000069"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR3938374.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12716..13882)
                     /gene="aroC"
                     /locus_tag="ON498_000070"
     CDS             complement(12716..13882)
                     /gene="aroC"
                     /locus_tag="ON498_000070"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3938375.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13884..14948)
                     /gene="aroB"
                     /locus_tag="ON498_000071"
     CDS             complement(13884..14948)
                     /gene="aroB"
                     /locus_tag="ON498_000071"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3938376.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14981..16003)
                     /gene="aroF"
                     /locus_tag="ON498_000072"
     CDS             complement(14981..16003)
                     /gene="aroF"
                     /locus_tag="ON498_000072"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR3938377.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16662..17636)
                     /locus_tag="ON498_000073"
     CDS             complement(16662..17636)
                     /locus_tag="ON498_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR3938378.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17617..19500)
                     /locus_tag="ON498_000074"
     CDS             complement(17617..19500)
                     /locus_tag="ON498_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR3938379.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19518..21743)
                     /locus_tag="ON498_000075"
     CDS             complement(19518..21743)
                     /locus_tag="ON498_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3938380.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21858..22892
                     /locus_tag="ON498_000076"
     CDS             21858..22892
                     /locus_tag="ON498_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3938381.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22959..23972)
                     /locus_tag="ON498_000077"
     CDS             complement(22959..23972)
                     /locus_tag="ON498_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3938382.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24050..24238)
                     /locus_tag="ON498_000078"
     CDS             complement(24050..24238)
                     /locus_tag="ON498_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3938383.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24678..24806)
                     /locus_tag="ON498_000079"
     CDS             complement(24678..24806)
                     /locus_tag="ON498_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3938384.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24809..26182)
                     /gene="rlmD"
                     /locus_tag="ON498_000080"
     CDS             complement(24809..26182)
                     /gene="rlmD"
                     /locus_tag="ON498_000080"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3938385.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26293..27348)
                     /locus_tag="ON498_000081"
     CDS             complement(26293..27348)
                     /locus_tag="ON498_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3938386.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27358..28788)
                     /gene="gatB"
                     /locus_tag="ON498_000082"
     CDS             complement(27358..28788)
                     /gene="gatB"
                     /locus_tag="ON498_000082"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR3938387.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28785..30257)
                     /gene="gatA"
                     /locus_tag="ON498_000083"
     CDS             complement(28785..30257)
                     /gene="gatA"
                     /locus_tag="ON498_000083"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR3938388.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30257..30562)
                     /gene="gatC"
                     /locus_tag="ON498_000084"
     CDS             complement(30257..30562)
                     /gene="gatC"
                     /locus_tag="ON498_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR3938389.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30668..32704)
                     /gene="ligA"
                     /locus_tag="ON498_000085"
     CDS             complement(30668..32704)
                     /gene="ligA"
                     /locus_tag="ON498_000085"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3938390.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32721..34955)
                     /gene="pcrA"
                     /locus_tag="ON498_000086"
     CDS             complement(32721..34955)
                     /gene="pcrA"
                     /locus_tag="ON498_000086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR3938391.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35110..36669
                     /locus_tag="ON498_000087"
     CDS             35110..36669
                     /locus_tag="ON498_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR3938392.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36780..38711)
                     /locus_tag="ON498_000088"
     CDS             complement(36780..38711)
                     /locus_tag="ON498_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3938393.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38729..39643)
                     /gene="pfkB"
                     /locus_tag="ON498_000089"
     CDS             complement(38729..39643)
                     /gene="pfkB"
                     /locus_tag="ON498_000089"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3938394.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39640..40392)
                     /locus_tag="ON498_000090"
     CDS             complement(39640..40392)
                     /locus_tag="ON498_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3938395.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40551..40760)
                     /locus_tag="ON498_000091"
     CDS             complement(40551..40760)
                     /locus_tag="ON498_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938396.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40771..42099)
                     /locus_tag="ON498_000092"
     CDS             complement(40771..42099)
                     /locus_tag="ON498_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938397.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42235..42390
                     /locus_tag="ON498_000093"
     CDS             42235..42390
                     /locus_tag="ON498_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938398.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42464..43051)
                     /locus_tag="ON498_000094"
     CDS             complement(42464..43051)
                     /locus_tag="ON498_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3938399.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43134..44384)
                     /gene="clpX"
                     /locus_tag="ON498_000095"
     CDS             complement(43134..44384)
                     /gene="clpX"
                     /locus_tag="ON498_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR3938400.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44631..45920)
                     /locus_tag="ON498_000096"
     CDS             complement(44631..45920)
                     /locus_tag="ON498_000096"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3938401.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46074..47006)
                     /locus_tag="ON498_000097"
     CDS             complement(46074..47006)
                     /locus_tag="ON498_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938402.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47163..47822)
                     /locus_tag="ON498_000098"
     CDS             complement(47163..47822)
                     /locus_tag="ON498_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3938403.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47825..48850)
                     /gene="sppA"
                     /locus_tag="ON498_000099"
     CDS             complement(47825..48850)
                     /gene="sppA"
                     /locus_tag="ON498_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3938404.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48863..49153)
                     /locus_tag="ON498_000100"
     CDS             complement(48863..49153)
                     /locus_tag="ON498_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938405.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49562..51367
                     /gene="glmS"
                     /locus_tag="ON498_000101"
     CDS             49562..51367
                     /gene="glmS"
                     /locus_tag="ON498_000101"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3938406.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51439..52794)
                     /locus_tag="ON498_000102"
     CDS             complement(51439..52794)
                     /locus_tag="ON498_000102"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3938407.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52818..53987)
                     /locus_tag="ON498_000103"
     CDS             complement(52818..53987)
                     /locus_tag="ON498_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938408.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53984..54871)
                     /locus_tag="ON498_000104"
     CDS             complement(53984..54871)
                     /locus_tag="ON498_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR3938409.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55060..58752)
                     /gene="nifJ"
                     /locus_tag="ON498_000105"
     CDS             complement(55060..58752)
                     /gene="nifJ"
                     /locus_tag="ON498_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3938410.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58951..59472
                     /locus_tag="ON498_000106"
     CDS             58951..59472
                     /locus_tag="ON498_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3938411.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60171..60728)
                     /locus_tag="ON498_000107"
     CDS             complement(60171..60728)
                     /locus_tag="ON498_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938412.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60907..61518)
                     /locus_tag="ON498_000108"
     CDS             complement(60907..61518)
                     /locus_tag="ON498_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938413.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61631..61888)
                     /locus_tag="ON498_000109"
     CDS             complement(61631..61888)
                     /locus_tag="ON498_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938414.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62485..62763)
                     /locus_tag="ON498_000110"
     CDS             complement(62485..62763)
                     /locus_tag="ON498_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938415.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62797..63048)
                     /locus_tag="ON498_000111"
     CDS             complement(62797..63048)
                     /locus_tag="ON498_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938416.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17699 a        13750 c        11220 g        20854 t
ORIGIN      
        1 aactaaaaaa ttcatttttc tccccctttt ttcatcattt ttaaaatagc atattttgaa
       61 aagaaagtct gatgcttaga gtagaatttc ataaaaattt actatttcat aagtattcac
      121 gagaaaaata caaacaaaaa agcggaaata acgctcatcc gccttcttgt ttgtgtttat
      181 ttcctcttca aacgcttgtc tatgattcag acttttctct atcgttattt tcatctaaaa
      241 gttcttgcat tccgataatt tctcgatatt gatgaatctt agtgatacca atgactttgt
      301 ctacatcaat actaaaacaa ataccattac ctggaatatc gatgttgatg ccttgaccaa
      361 tcgcatccat tactttattc gtcaaactat cgggtactaa tgtcaacaca atatcttttt
      421 ctggcgaaat gtccaaacct aaaaatttcc ctgaatcatg tacaccagaa cctcttccat
      481 gtaagatcgt tccgcctgct gcacctgctt cttttgaaaa gtcgataaca tcttcgccca
      541 ttcctccgtc aacgatcgtc acaatcatgt gaagattcaa tgctacaaga tctttcattt
      601 gctttccccc aatttttgca atctttcttt ttcgattctg tctttttcat cttttcgttt
      661 ataaatatat cctagtacta aagtggataa tattggtgtc aatgcaatca ttgcaaccac
      721 accaaacccg tcaatgatcg gatctcttcc ttcaatttgt gcagcgattg ccacgctcat
      781 tgataaaata aatgtcgaac acatcgggcc cgttgctact ccaccgttat caaaagccat
      841 tgctaaaaag atcttgtcca cttttcgtcc taaaataaat gctagcccgt atcctgggat
      901 caagaaataa taaagagaga atccgacgag caaacgccac atagacaatc ctactgctgc
      961 gccgatccca attgaaatta ccatcagcat gattttcgat cggattctcc cctcgctcaa
     1021 ttcttcaacc tgtttgatca ttacatgaat cgccggctca gcaaacccta ctaagaaccc
     1081 cattaaaaag ccaaggggaa ccagtatata attgtaaggc aatcccgcaa tagcactgcc
     1141 taaatgctgt cctaccggta cataagccat attgactccg tgcaggaaaa ggatcaaccc
     1201 aatagtcgtt atcaagaaac ctttcataat gtcaaagaaa cgctttttgc gcatacgaac
     1261 taatgatatt cattaaaaca aataggatta cgataggtgc cactgccaaa atgacttcca
     1321 tggtaacgat ttcgattcca tctatcactt gctgccagaa actcattgga agatcacccc
     1381 caaaatcatc acggcaagaa tcggccctaa cgaagcaata ccgaccattc caaaactgtc
     1441 attttcattt ttcttttgtt tgatcatact agtcatccct ccggcaaggg ccaagataaa
     1501 tggaacagtt actggaccag tagtcactcc ccccgaatca aaagcaatcg gcataaattc
     1561 ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag cctatagcca tcaattgata
     1621 ataggatagc ttgaatacga cccgaagcag cgcaaaggct agaaatactc cagtgccgac
     1681 gctgacgatc ccaatcagca acactctgcc aatctctccg tcagatatat catatacttg
     1741 ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc acgatcccga tagcaaatcc
     1801 caaactgacc aaaacggtca aacttttctt tttgatcata tatttcccta ctagatctcc
     1861 gacttccatc atagaataat ctgctccaaa caaaaagagc gtcattccga tcatcatgat
     1921 tgccgcacca accagaaatg aagcgaggtc ttctccttct aacggtgcaa aaacaaacgt
     1981 taaaatgaca atcaggacag tcatcggcaa tatagcagca atcacttctt taaaattttt
     2041 tagcatgtat cctcctcctt tactctttaa gtttacctta aaatgaaaga gttttcgagc
     2101 acaaacttaa aacgttatca tttaattttt ctcaacataa aataacacac gtcccaaaag
     2161 tatcttttta cttggaaaaa tgactttgaa ggaacgcgct tattgtgttt ttttatttta
     2221 cagagccgat cattccttga acgaattgct tttgcaaaac aaaaaatacg atggcagttg
     2281 gtaaagtagc gatgactaat ccagccatga tcattccata gtctggtgta taggaagcac
     2341 ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg agattgcaaa gctactaagg
     2401 gccataaata attgttccag ctactcatga atgtgatgat tgcagccgcg gcataggtat
     2461 ttttagccgt cggcatatac actctaaaaa aaatacttag ttcttttaga ccgtctaatc
     2521 gagcggcttc tactagatct tttggaaaag atttaacatt ttgtcgaaag aagaagatca
     2581 aaaaagcagt gctaacagat ggcaggatga caaccaagaa actgttgatt cccaaaaccg
     2641 ttccgttcaa ttgtgaaaac attcgatata aagggatcat caatgccgca aaaggaacca
     2701 tcatcgatag cagtagaata ttgaaaatta ggtcttttcg tttactttga taaatctcaa
     2761 atccatatcc tgccattgac gaaatcagca atgcaaacac agtggtcacc aaagcgatca
     2821 tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt ggaaaacaaa ttttgaaaat
     2881 tgatgatcaa gttactgcca atctttattt ttcccgatgt gatatccacg ggtgtattcg
     2941 tcattcccaa aaccatccag acaaaaggga aaatggaaat aaatgatagg atgcttaatg
     3001 ctgtatactt taatactgac ccaattgtcg aacgaagttc taatgcttgt tttttgacgt
     3061 tggggcgttc gattgtttct ttttctacca tatttttcat cgactttctc ctcctacttt
     3121 cgtttggatt atcgacaata tcacaataaa ggaaacaata acaaaggaaa ctgctgccgc
     3181 atatccaaaa ttaggcgtga atttataact caaattatag atgtattgag atagggtggt
     3241 cgttgcattt gctggtcccc cgccagtaat attttgtact tcatcaaaaa gctgcaatgt
     3301 cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt tttaaatttg ggatcgtcac
     3361 tttgataaac tgctgccatt tagaagcacc gtcaatagat gctgcttcat aaatctccga
     3421 atcaatattt tgcatgcctg aaaggaaaaa aatcatattg tatccagtcc aacgccaagt
     3481 gatcgaaaga atgatcagaa ctctcgccca aattggatga gtcaaccagc caatcggtgt
     3541 gtcaatcata ccaatgccca acaacaagtt gtttacaagg ccagattgag aaaatagact
     3601 tttcatgatc agagaataac tgaccatcga agtgatacat ggaagaaaga tagcggttcg
     3661 aaacaaccct ttatatttca actttttatc atttaaaagg ttggaaataa aaagtgctag
     3721 aaacaacatg atcggtactt gtacaagtaa atacaggaat gtattaaaaa aagctttttt
     3781 aaaagttgca tctgtcaaca tccgctgata attacctaac ccgttaaatg tcatattgac
     3841 agggggacca ctttgaaaag acgtgatcaa agccaaaacc atcggaataa aaaccatgat
     3901 tgatatcaaa atgactggca agattaaaaa agcatgcccg tttttgaata aatctttctg
     3961 cttcattctt ttccctccaa ttctatcatt gattatctac atgaaaagac aagaaagaca
     4021 atatctgtaa ggcagacgtg atcttttttg tcttttcgtt tgtctgatga aaaagcgaag
     4081 aaattcactt ttttaccacc gatcaattag ccaattgtgc ttctacttgt ttttgtgtat
     4141 ctgctagtac cttgtctgtc tcttctccat tgatgattcg atgaagagct tcagctacga
     4201 ctgcttcaat tgcataggtc tcatgtccgt aattcacttc tggtatttca gatgtccatg
     4261 ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc agaagcttct tgataagctt
     4321 cttgttcatt cgcgctaagc atcgtagata ctagtccgat ttctttcgca agagtgccca
     4381 tcagttcttc attcgaagca aacgttgctt ctaggaaatc tttcgctgaa tcttctccag
     4441 ctactccttt gatcacatac cagccagcgc cgccaaggtt agaagcttgc gctttttgca
     4501 gattttctgg aagtgcaggt attggtgcaa tcttccattt tccagattgg ttttctgctc
     4561 cttggatcgt tgaagaatac caagcgccag ttgggctaga agctactgct cctgattgta
     4621 tcgcagttac tccagcattc cagtcagagg tttgttcgac tagatcttct ttcaaaagag
     4681 tggcaaacaa ttcaagacca tactttaaag atgcattgtc ttggatcgtg actgtctccc
     4741 catcttcaga tgtataccat tctcctgcct gttgcataat catccttact cgaccaaggt
     4801 cagaaggatt gacttcagcg attttctttc ccgtcttttc tttgacatct cgcgctactt
     4861 gaatatactc gtcccacttc agattctcca tatcctcttc tgtataacct gcttctgcca
     4921 tcagatccgt acgatagaag tttgctgtta cgcccgaatc gaaaggaaca ccgtatattt
     4981 tatctcctac tttgtttacc gcaaatttat acggtgcaaa atgatcttcc tttacaatag
     5041 cagacaaatt agcgaatgca tctggataag aagtcaaata gccttgtatc ctataatctt
     5101 caatcaagac gatattgggc aatccttctg tattcccgct cgctaaagcg gtgttcagtt
     5161 tctgaacgat atcatcctga gacatcgtaa caacttctat ctctgtttct tcgttctcat
     5221 aaactttttt tgcttcattc actgctttga tattgaatgt ctcatcccac gcccagacag
     5281 tgactttttc ttctttgtct gctgtactac tagcacttcc gcatcctgtt aagaaacctg
     5341 atgctgccgc taaacaaacg actccagcaa ttaactttct gatgttcatt tttatccgct
     5401 ccttttccat ccattagttg tttacgtttg ctacattcat cataccaaaa tttgaaaacg
     5461 gtttctttat tattcagata cttcaaaaat ttctcctaaa ataataaaaa aatacaaatc
     5521 aactatgaca aaaggaataa tcttagcatc tagaccaaag atcgaaatgg tgaggtttct
     5581 ttaaaataaa aatatccttg cattttctcc tagaaataag tatttttaaa ttacaagact
     5641 attcttaagg cgggtaaaaa aatgccaata cgcgtaattc ttatcgatga tgaacagacg
     5701 attttagatg gaatgaaaca gttgatcaat tggtctgagc acgggtttca attaataggg
     5761 actttttcca gtccgctgaa agcgcttgat ttttctttgg tacatgcaat cgatatcgtg
     5821 gtcacagatg taatgatgcc tgaattaaat ggaatcgaat tatcgcggct attaaaacag
     5881 caacaacctg aaacacagat ccttatcctc tctagctatg atgaatttga tatggttaaa
     5941 gattctttta aagaaggcgt cgctgattat cttttaaagc ctcgattgag tcctgatttt
     6001 tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca
     6061 atcaccaatc gttatgagca gatctgcgag aatctttcac agcagattgc tggacaaact
     6121 cgtccattgc ttgaccctct tctttttgaa cacaccttgt ttttctgtta tataccgaaa
     6181 aacggccatt cagttcaaaa caaggctcta aacaaaaaca gtcgatcacc cattcattga
     6241 tggatagcga cgattcgttt actatttatc ctgtttctac cttttcggaa gaatccggct
     6301 atgtaatcaa tactgattcc aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag
     6361 aagtttttcc aggtgatttt tttgttgttt ctgattcttt tgcaatccaa gatatcaatg
     6421 aaatattcca agagatcaga aattgcacca aaggacaacg tttctatcat aagggaaaga
     6481 accttgctta tcagcatgaa cttcttccgt tattaaccaa ccacgagcag caagcaagta
     6541 gttatttaac aaaaatcgtt cataaagact acgctttagc tgtcaaagaa cttgagaatt
     6601 tcttcacgac gatattggaa cagcctattg atccagctta tttgaaacat gaggcaatca
     6661 atcgtttcta tgcattactc aacgctttgg ctgacgagca tgttgggaat gaagaacttt
     6721 ctttgctcaa gatcgccctt ccttctttat tagccgatgc tgagtatttc gaagattttt
     6781 ctattcttgt caaagaaaca cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag
     6841 acaatttcga tatcttgact ttgatctacg agttcgtcca ggaaaattat gagaaagaaa
     6901 tcaatttaca acttttatcc gaaaaatatc atttttcata tagctatctc tcagccatct
     6961 ttactgaaaa atttggtatc aacttttcaa aatatctaaa aaaagtccgt atcagtaaag
     7021 ccaagctctt tttgacggaa acacggctta ctttgacgga aatctgtgag aaagtcggct
     7081 ataccgaact gggttatttc tccagagttt ttaaagaaga gacaggaatg acaccttctc
     7141 aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc
     7201 caaacttctg ttcattttgt tcatcggtct ttttctgcaa gccatcgtca tttctttgtt
     7261 tatttatcat cgatcaaggg atgcctatat ccagctgttc aatcaatcga atgatgtggt
     7321 actaaaaaag attcaatctg aatttgaaag tttgaatgat acaatcgaga atacattagc
     7381 cgcttttgat tctaatccag ctgtcaaaag ctatttttcc aatgatccag cccaacatat
     7441 ggaacagctt cagcaactaa gaacaatcca aaaaatgaat gactctcttt caaaaatcca
     7501 tccaatgatc gattacgatg tattgatctt tggagaaaat ggaagaactt ttgtcggaaa
     7561 cgacatgctc actgccgtat ctgccgattc tttttttcaa tctgcgattg ctcagagagt
     7621 gaatgaacgt gctgctgata cacaaatgct ttttgcacac cacggattga ctcttcgcga
     7681 taagaaagca cccagcgtct tctttgtcaa aaaactgaaa aatacattga atcacgttta
     7741 cggatatgct gttttatcga tctcatccaa gcaactagcg aatctttttc aatctgtagt
     7801 aaatcctgag atatcgaaga tctccttcat gaacgaagaa cagacgatca tcgcatcaaa
     7861 tgaagaaaat acaattggga gccgatcaat gattttcaaa aaactaacag taggtgaaac
     7921 aacgatttca aatggacagc agttgacccg tcttcctctg taccgccagg attgtacatt
     7981 actcagtcaa gtcgatcttc attctttatc taaccaaatg ggcgtgattt ttccgattat
     8041 tttgtataat atcgtgacac ttgcttttgc tggctcattg gtatttatgt atttggatcg
     8101 acacacaaaa tcgatctacc gcttgattga ttcgctaaaa cggctagagg aagatggctt
     8161 aaaagctact gttcctgtac aaggaagcca tgaagtccaa ttgctaggga atacgatcaa
     8221 tcaattgcta agtaatgctc agcatcatta tgaacagacg atccaagacg aaaagaagaa
     8281 acgttcgctt gaaatccaaa ctatgcaagc acagatacag ccccatttta tttataatac
     8341 gctgactagc ttgaaatttt tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat
     8401 cgacagtttc attactttac taagaagtac gattgggaaa aaagaagaag tgattccagt
     8461 caaggaagaa atcaaaagcg tacaaagtta catcgatctt ttgagtatgc gatatggcga
     8521 aagaatctcg gctaaaataa tgattcctga tgaactgcag tcattatcta tgccgaatat
     8581 gatccttcaa ccaattatcg aaaacgccta tcttcatgct tttcacaata aaacgcaagg
     8641 attcatcacg gtgtacggca aaacaaaaaa tcagcttctt ttattcgaag tgattgataa
     8701 cggcgatggc ttcgatactg aaaataccaa acaaaaaaat gatcattttt caggaatcgg
     8761 catcagccat gtagatgagc ggatacgcct aatgtatggc gatgcatatg gcttaacgat
     8821 ccaatcagtg attgggacag gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa
     8881 tcaatcagaa tatcaaaaat aaagaatagc caagcgctga tttttttatt ttaagttaaa
     8941 taaatcaatc agtattcttg actttcatct acgcgtctct tctactgtca gcactggata
     9001 acagcccaat atttgacttt ctcctccgag catcttgatt tcttggagcg cattctctac
     9061 taatgtcatt ggacggtcca acaacaaatc gatcacgaaa aaatattctc ccaagctagt
     9121 tttcaatggt cgggattcaa tcttacttaa gttgatttcc cgccagccaa aagctgccag
     9181 cattttatga agcatccctg gccgattagc aggtagtgtc aagatgacac tcatttttac
     9241 tggagctgac tgctggctgg tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt
     9301 caactcatta tcttggatat tctcagctaa gatctccaat ccaacatgct gtgctgtttc
     9361 taatgaagca atcgctgctg cgtcttcttt cggatgttct gctacataca ttgctgcagc
     9421 agttgttgat tctgttgcta ctaaaggaac attaggataa tgcgtttcta aaaactgttg
     9481 tgattgtgct agtgcttgtg gatgtgaaag gatttttgta atctttgttg ctggattgcc
     9541 taaaagctgc tgcttgatag gaagaacgat ttcactttta acttcgactt ctggatgttt
     9601 ggaaagaaga tcgattgtgt gatgaacagc tccttccaat gaattctcta caggaacgac
     9661 agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc
     9721 agtcaattgt tctgtaggaa atagctgaca agccgcctga taagtgaatg aactttctgg
     9781 ccctagatag ctgacgttca tatgcttttg atcctttcta taatggtatc gatgatcttt
     9841 tctggtgttt gattggtcgt gttgattcga tgtgtcgcac tttcttcata ccaagaaagt
     9901 cgttgtgcca ataatgcttt tatttcttct cttgtttttc caagagctaa aggtcgaata
     9961 gacgtttcat cttgttggat acgatcaact agacagtccg cttctgcctc taagtaaaga
    10021 acaatcggct gtgtttttaa aaaacgccga ttttctgcac tttggacaat ccctccacca
    10081 gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa tatctgactc cacttttcga
    10141 aagctttttt caccgtaata gtgaaaatat tcggcaatcg acaattggat atgttcctca
    10201 atctttgtat ctaaatctac atactctttt tgtaaacatt gagctaaccc tttaccgatt
    10261 gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc tagccatgag cttctcctcc
    10321 tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc
    10381 tccttctagt tcgcaaggtc catcagctat caaagcagct acttgcagca tcataccgat
    10441 tcgatgatcg cctctgctgg acacttttgc gccatgcaga ggtgtcggcc catggatgat
    10501 cagaccgtct gctgtcgctt ttatctttgc tcccattttc tgtaattctt ctgccaccgc
    10561 atcaattctg ttggtttctt ttacttttaa ttcttctgcg tctttgatga tcgtgattcc
    10621 ttctgcttga gtagccacta aagctagaat gggcaattca tcaatcagac gcggaatgat
    10681 ttctccacca atcgttgctt tcttcaaatg actcgtcttg acacttaaat cagccgattg
    10741 gttctgttca ttgtgattcg tctgggtaat cgctgcgccc atctcctcca acacatctaa
    10801 gataccagtt cttgttggat tgaccccgac attttttaac aacagttggc tttccggaac
    10861 aagaagtcct gctgctagaa agaaagcagc tgatgaaata tcacctggaa cagttatctg
    10921 ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct
    10981 tccgccaaat tgacgaatca tttcttccgt atggttacgg gaacgttctt tttcaacgat
    11041 tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa gcagatttga cttgcgcact
    11101 ggctacaggc atattgtatc ggataggtga taaagagcgg gttcctgtca cagtgattgg
    11161 tagatactgg tcattttctt ctccttgcaa gtcagcgccc atctcgcgta aaggttccat
    11221 cactcgcccc attggacgtt tagacaacga agcatccccg aacagagtag tcgtaaaagg
    11281 ctgtccagcc aaaataccca tcaataaacg tatggtcgta ccggagtttc ccatgtcaat
    11341 tgattcggtt ggttggacaa atgagtggct accgcgacca tgaatcttga tcacctcttc
    11401 ctcttcttcg atctcgacac ctaattgttg gaaggcatgt aaagtactca gacaatcttc
    11461 tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca attgctccaa acatgatgct
    11521 gcgatgggaa attgatttat ctgctggtat cctaacagtc cctcttaatc catgtatttg
    11581 ttgtaataat tgcatttatc cagcctccta tttgatccag caatgataac tggtattaga
    11641 cataatgatt ctttttgctt gtaacaaatc ctgttggttt ttaaaactga tttgcagtat
    11701 tcctaaaata tcttccctgg tctcttggat cttcaaatta atcaaagaaa ctcccgcttt
    11761 tccgatcagt ccagttactt ctgcaataac gccaggttca tctggcacat cgataaataa
    11821 atcaaaaaag gcaggaatcg cgccttgctt atggacaggc aactggtctc gactttcttt
    11881 tgcccgattg aaaaaagaaa agatttccga ttggttctca gtcatgatcc attctgctat
    11941 ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat aatatctgct tattgctcaa
    12001 caaaatatct gtccacatga caggatctga ggaagcgatc ctagtgatat ctcgaaagcc
    12061 tcctgctgcc agttgtttgg ctcgcggata ctcttgattg aattgttctg cctgattgac
    12121 taagccagct gccagaatat gtggaaaatg acttagcatt cctgttatac ggtcatgttc
    12181 tcttgctgta agagttacat attttgcttt ggttccgcaa aatagttctt gcagtttttc
    12241 aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa taggcatttt caaatagttt
    12301 agcatctgct gcctgtatac ctgatttatg agaaccagcc attggatgtc caccgacaaa
    12361 ccgaagattt ttctgctcag cggatgtcac gacttgctgc ttcgtactgc tgacatccgt
    12421 aatgatgacg tgatcagaca agttgattct ttcaaggaca tcaagataag ctaaggttgt
    12481 acggacggga acggctaata agataacgtc tgcttgttct gcacctgttt caaaatcact
    12541 tgcaacgtaa tcaacgatct gctgttctac tgcttgctca cgggtttttg actgactatc
    12601 ccaaccaact actatcagtt ctggattggt ctgacgaata caaagtgcca tagaagcacc
    12661 aatcagtcca agccccacga taaaaacatg ttgtgccatc ttatttgccc cctttttagt
    12721 aatgtttcaa gtagtcacgg tactccttca ctgcagcttt caactcaaca agcgaatcac
    12781 ttgggaattt tgacaatagc tctgtcgcta ccacagtggc tacaacattt tccgcaacga
    12841 cgctagctgc aggaactgct gtactatccg aacgttctac acttgctttg taaggttctt
    12901 ttgtatcgat atcaacactt tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc
    12961 cgcgaatgac aagtggttcg ccattggtca ttccgccttc gaagccgcct agattattgg
    13021 tacgtcggaa atagcctctt tcttcttccc ataggatctc atccatcact tctgagcctg
    13081 gtaatgaacc agaagtgaat ccgaccccaa attctacgcc tttaaaggca ttgatactaa
    13141 ccacagcttg tgcgattttg gcatctagct tcgtgtccca ttgtacgtag cttcctaatc
    13201 cagctggaac gcctccaacg agcacttcta caattcctcc gatcgtatct ccatttttct
    13261 ttgtctgatc gatcaaatct tttattcttt gctcaactgc tggatcaaca actcgtactt
    13321 ctgacgcttc agaaatttct ttgatttctt gaatcgagat atttccaggt atttctgctt
    13381 ggattccacc aagacaaatc acgtgtcctg ctacgatgat gtcaagttct tccagaattt
    13441 tcttggcaat tgcgccgatt gctacacgca tggtcgtctc tcttgcagag gaccgttcaa
    13501 gcacattccg aagatctcga tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg
    13561 gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc aggcacttct gctacagaca
    13621 tgacagttgt ccaatttttc caatctttat tttctaccac taaagtgaca ggtgaaccaa
    13681 ttgttttgcc gtgacggatg ccagacgtga tccgaacacg atctttttcg atcttcatcc
    13741 ttcctcctct gccgtaacca gcttgtctgc gagcgagttc gtgattgatt tcttcttctg
    13801 acagaggtaa tcctgctggt agtccttcaa taatggctgt taattctggt ccatgggatt
    13861 ctcccgcagt catataacgc atcttacagt cctcctaagt aatctttgaa tgtctctgtg
    13921 ggaacaggaa cgatttttgc ttgcccaatc ttttctagca agatgatatt caattggctt
    13981 cctcttgctt tcttatcatg ggttatcgcg tggaataatt catctggatg acttagatcg
    14041 tacgtaatcg gcagatggaa tttttgaagt atcctttcta aagcagctgt cgatccagtt
    14101 ggagacaagc cgattttttc tgcatgagaa gtgatggcta ccattccaat agctactcct
    14161 tctccatgac tgactacccc atatcctgct gtattttcaa ttgcatggcc aattgtatga
    14221 ccaaaattta aaataagtcg atttccttgg tcaaagacat cttcttccac tacttttcgt
    14281 ttgatttcca acgtagcagt aatgattgga acagcatgtt ccagaagatc tttttcgtct
    14341 ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta tagctgctga tttgatgatt
    14401 tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt ttagaacctc aggatcaatc
    14461 aatacaccag ctggctggct aaatgttccg acaaggtttt tcgctttgtt tgtattgact
    14521 gctgtcttcc cgccaatact agaatctact tgtgcaagta gcgtagtcgg aatttgaacg
    14581 aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg ctagatcacc caccattcct
    14641 ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat
    14701 aactcagctg cttgttctaa agatttactg gtctcacccg ccggtacgat tgaaaggact
    14761 ggttcaaaat tagcttgtct caattggaac atgatttttt ctgcatatag tggagcaacg
    14821 tttgtatcag tgatcacagc tactttttga ggtctccaaa tcgtttgaag ccatgatcca
    14881 gcctgttcta gtgcgccttt ttcaatatga atatcgtatt tttggttatc aacatgtaca
    14941 gtaagcattc gtttcacttc tttcttttaa aaagctggaa ttaatcgagt gtctgaatct
    15001 tttccattgc ttctttcatg atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat
    15061 tcaacgattg gggaccgtct gaccaagcat ttgcaggatc aggatggatc tctacgatca
    15121 tgccatctgc tccgcttgcc acacccgcac gagccattgg tggtacaagt tcccaaattc
    15181 ctgtaccatg acttggatca acaatcacag ggaaatgagt taattttttc atcaatggga
    15241 cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga
    15301 taaaaatgac aggagatttg tcttcgactg caatgtattc tgcagcatta agccattcat
    15361 tgatcgtacc ggaaattcca cgtttcaatc caacaggttt acctgtttta ccgacagcag
    15421 atagcagttt aaaattttgc atattacgtg cgccaatctg taggatatcg ctgtactcag
    15481 ccaccatatc gatatgccct tcatccatca cttcagtaat cactttcatt ccaaattcat
    15541 cagccgcttg acggatgtat ttcaatcctt cttcttctaa accttggaaa gcatatggag
    15601 aagtacgtgg tttgaaggca ccacctcgca gaatttttgc gcccccggct ttggccattc
    15661 gcgcacattc tctgatttgg tctaatcctt cgatagaaca aggaccagcc atcgtcacga
    15721 aactgccgtc accaattttt actccgtcta catccacgac tgtattgcca ggatggaatt
    15781 ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc attttctact ccttcatagc
    15841 gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt tagtccaatc actactcgtt
    15901 ctgttccttg atcgattgag actttgaatc cttctttttc gatatgatcg acgacttttt
    15961 ttatttgttc ttccgttgct tcttttttca taattactat cattgttttt cccgctttct
    16021 tttgtttact tatctatctt ggctgatttt ctctactttt tcaaggtggt tttacattaa
    16081 ttcctactaa atgaccgtgt cctgttgttc tcattttcgc tccttctttc ctacttatga
    16141 cccagataat caacaaaaaa agatgtccct gtaggaaatt gcctctacag tgaacatctt
    16201 caacgagcct taaaaagcgc tgcaaaaata taaaatagcc actatagatg aatttctcta
    16261 cgtggctgta agtcgtgttt aaaaatcttt agccatcata gatacaaacc tggtttgttt
    16321 gtacccacga agtcatgaat acaaacctat ctaaaatagc taaagtaata cgtattcagt
    16381 tttgtaagtt ttctagattt cataaaaaat cgctccaaac tgatgtaaga attgtaccta
    16441 gtatagacat aaaaaaatcg actgtcaatc atttttatga aacttttttt aattttctga
    16501 tagttaactt tgtttttcag aaaaataaca attggtttta ttgaagaaaa tagtcatgaa
    16561 caaaagtatc tgagaacttt tttactctct tgttcatgac atatagaata aagcagcttt
    16621 gtcttgtaaa ctccgttttt caaataataa tctaaagatt gctactgaat aataaagccg
    16681 atttcatggt cctcttcgct gctgattcgg taaggtgact caacatccgc tccccagcta
    16741 ttgatacccc cgactcctct tacttttgca aaaatcgtaa gaaccgttcg tctttctggc
    16801 ggtagttctt ctatatgtgt tgcctgttcc agttcttctg ctttgaatgg taaagcggaa
    16861 aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc
    16921 gtattatcca acgtttgatt tcttgttagt tctacccatt tcgtttccat atgcatgccg
    16981 cattcttggg gaacaaggta tggggaaacg gggagtcctg ccactttata aataccttcg
    17041 acaccaccag ccatccgatc cggatatgtt tcccctgata ggccttcata ctcaaaaaat
    17101 tttgctttcg ttggcataat gaaccttaaa cccaatgcgg gtaattccgg taatcctttt
    17161 tgtccataat aatgcatatt gacaagcatt ttaccctctc cagcgatttg gtaggaaatt
    17221 gtcacttggg tttttggtac tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca
    17281 gcgtattcat tacttgaata ttgattattt actggagcaa tcggcaaatc gatcttttta
    17341 ttgtccacca gtacttctat ttctttgact tttggataca atccggctcc taaccacata
    17401 ccggatttaa atccaaatcc gctacctctg tcattatcgg tcgttgctcg ccaaaaaact
    17461 ggaagtgttt cccggtatag ccattctttt ccgtttttat tgagcgattc cagtccgcct
    17521 cttgtataag aaaaaatata atgcgtattc cccgatttga cacctaatgt acagtcgccg
    17581 aagacgattg caactttttc cgtatccata tgacttttat ttactgtatt tttcataata
    17641 atatcttacc tcctgcatgg cgggtttttc ttgtccatct gcaaaaacga tcccattccc
    17701 tgagaattca tagtcacttg gtttatcgtc aaagtcaccg ccatagcgca tcacttctct
    17761 gcctgtgatt ggatcagtca cttggatcgc ctgatcttta aaatcccata tatagcctcc
    17821 ttggtacatt tcataacgat caagcaacgt catgtaagaa tccattccac ctaacgaatt
    17881 ccccatgtca tgcatatatt cacataagat gaacggtttt tccgctttat tttcaaggta
    17941 gtcaatgatc tcttctggtg gcgcatacat gcagcttttt acatctgaaa tcgaatcttc
    18001 gaatgctgga tttggaaaat tcccttcata atggacaagt cgatcggggt ctactttttt
    18061 gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa gattcatttc caagagacca
    18121 aaaaagaata cttggatggt tttttaatgt ttcgaaattc gtttttgctc gatcaagaac
    18181 agccgcctgc cactgaggta ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat
    18241 tttttgccat gagccatggg attcgaggtt cgtttccgcc atcacatgga ttcctgcttg
    18301 gtcgcataaa taataccaag tgatctggtt ggggtaatga gatgtccgta cgccgttgat
    18361 ctttgcattt tttatgatag ccaaatcttt ttccatatct tctatagtga tggcgcgtcc
    18421 ttttttcgga tgccactcat gtcgatttac cccgttgata atcatccgtt ttccattgaa
    18481 gtagatgatt ttatctttga tttcgatttt acgaaaaccg aaggaatagt caatatactc
    18541 cgttactctt ttttcttcgt ctatcgtctc caattcaagc ttatataaat tgggctgaaa
    18601 atgcccccac agtgccacat tttcaaatcc atcgatgcga atcgtcattt gctcttttaa
    18661 cggatattgc tgttgataga tttccttccc atcaggatct agtatcgcta cattcagctg
    18721 actgtttttg gctacttgct caaaagacaa atctacttca agtttgccat tttgacattt
    18781 gtcatctaag cgaggtttga tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa
    18841 gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc
    18901 tgtacttctt ttgtaaacct ccacagctag aatattttct tcttccaaat aaggtgttaa
    18961 atcaaattca gacggagtga acgaatcttc ggcatacccg ataaaatgac cattcaacca
    19021 aataaacatt gcttgttcta caccagcaaa atgaatcgag atttgttttc cctttagacc
    19081 ttcttctaat cgaaaagtcg tcaaatacga accaaccgga ttgtctttag cagctgaaaa
    19141 gatcccttcc gcctctttct cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc
    19201 ctcccacgga tacatcgtat tgatgtaatg gatcttgtca taatttgcta attcaatatg
    19261 actaggaaca gtgatttcat caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt
    19321 tggtcgtacc gaaggatttg gcgaataaca aaaacgccat gatccattta aagactgttt
    19381 aagggaggat gtggtttcta cttcttccct tgttgcataa caaacatgat cgctatgtgc
    19441 atttgattgg ttcactctaa aaacttgtgg atcatctaac cattttatat ttgctttcat
    19501 acttttttcc ttctctctca taactttttg aaatgaagca gttctgtttg gaaatcacct
    19561 tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg cccccgaaaa tatccgcttc
    19621 gaggtctgtt cctgataaag tgcctcatca tctaacccca tcagatatac ttcatggaac
    19681 gctggttgag ctgaagctag tatcctccct aaaaacacaa tagcttcatc ttgtttagga
    19741 gacacaaaca tccatgcggc aatattttct tcaaaaggac tcgccaaacg ataataatgg
    19801 ccatactgga tcaccggacg aatcgtttga taggatataa tttggtcttt tacttgcttt
    19861 tcttcttcag gagacagctt tgtcaaatcc agttcataac caaaaacagc gctcatagct
    19921 gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct gatgatttgg tacagctgat
    19981 acatgagctg tgatggacga aatcggataa gcaaggcttg ttccatattg gattttcata
    20041 cgctccactg catccgtgtt atcacttgtc catgactgcg gcatataata aataaacccg
    20101 gcatcaaacc gtccacctcc accagaacag ccttcccata agatttccgg ataatcagtt
    20161 gtcaaatgtt caagtagttc ataaagcccg agcatataac gatgcgctac ttctccttgt
    20221 ctctgtgggt caagggtaat cgaatagaca tctgaaagcg aacggttcat atcccatttg
    20281 atgtagtcaa gaggaatcgt atctaaaatc tttctcattt gtttctcaat cgtctgccga
    20341 acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg aagcagaagg cattctacct
    20401 ggctcctgca taagaaaatc aggatgtgtt cgatacagtt cagaatcaac cgagatcatt
    20461 tcaggttcaa accataaacc aaatttcaat cctttgctat gagcataatc tgctatttca
    20521 cgtaatccat ttgtcaactt gccttcatac tcgaaccagt ctcctaatga agaattgtcc
    20581 gcatctcttt ttccaaacca gccatcatct aatacgaaca tttcgatgcc tagctccttt
    20641 gcttcatcaa tgatcgcctt gattttttca ctattgaagt caaagtaagt ggcttcccag
    20701 ttattgacca gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt
    20761 agcaactcat ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc
    20821 atttcaggtg tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg
    20881 atcccaccga cgactctcaa ttgattgatc tgatcttttt caagttcgaa agaatggttg
    20941 ccggaataaa caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga
    21001 actaaagcaa taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact
    21061 tttgttccag ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc
    21121 aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagccgct
    21181 ttttctagtt tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact
    21241 gctctatcag taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta
    21301 agtgaaatca ccaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct
    21361 tcgatctctg gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct
    21421 gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa
    21481 gagtattctt gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt
    21541 cctgaaaatc cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt
    21601 ttgccaaaat aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga
    21661 aatgtatttc gtaaatggaa gactcggctc ttttcatcaa ataagatgaa atttttatct
    21721 gtttttctat gcgtaatatc catcatttca ctcccaacaa aacggaaacc gtttactttt
    21781 ttttataatt cctgtatact gtttactaaa caattttgtc aataaaaatt ttttattttt
    21841 ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc acgaaaggca aatgtctctc
    21901 ctgctacagt ttcccgtgtg ttgaataacg atcagacgat ccatgtggct cagactactc
    21961 ggcaacggat agtagaggca gctgctgcgt tggattatga catcagtact cgtaaatata
    22021 tcaaaaagag aatgccttcg attggtgtga tctctacgat cggcaaagcg agcgaagaac
    22081 aggatatcta ttacaaacat ttgcgagtag ggatggaaga agaagctcga cgtctgcatt
    22141 tagggatgaa tcgaatctat aatctatctg ataatcctaa acaatgggaa gatctagatc
    22201 agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga atcgattacc gccttgttaa
    22261 aacaaaacat gcaaatagtc gtcgtggata accctgacat ccagcaagag gtcgatatgg
    22321 tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga attgttctta gaaaatggtc
    22381 ataaaagaat agcctatatc gggggatata aagtcgatat ggatgaattc ggtgggaagc
    22441 agatttctat aaatgaaaag aggatgcgtg cctatcagca ttttatgatc gatcatcact
    22501 tagaaaaata tatgtcttat catctaggtg gctggacaga aaaacaaggt gaggtacttg
    22561 cgactaatct attagaaaat gaatcccctc ttcctactgc cattcttgtc gggagcgatc
    22621 caatggcaat tggtgtctat cgggcattga aacaaaaaaa tatccaaatt ggagaagaga
    22681 ttgttgtagc aagctttgat aatatcgaga caatcgcaaa cctctcccct ggcttgacca
    22741 gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt tcgaatggca ttggagcgaa
    22801 ttgacggtat acgggacgaa ccaatcattt tgacttatcc tgcacgattg attgttaggg
    22861 aaagttttat cccaagaaca aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt
    22921 tcttcatact caaaacgaaa cggctttttc acaacctctt acttatactt attcacaaaa
    22981 tccaatgtct gctggtaaca attttgcgat tcttttagcg tgtaatcaaa ttggtattca
    23041 tgggtaatct ctttttttgt atctgtataa aacaaggatt gcacgggtac ctttaattct
    23101 gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt
    23161 atatacgttg gcggaaattc cttagaaaca tggtcagcca cactagcttc tttcaattgc
    23221 tcactcgttt tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac
    23281 ttcataaatc gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa
    23341 taagaaatgg ttcctttcag actatctttt ggaagtagct gtttgatttt catttgttta
    23401 gcatacgcat cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta
    23461 tctccaccaa aaaataattg ttccatgtct agcaatttgt cgtctttttc ttcagctaat
    23521 aaatctttga tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct
    23581 aattcataat tcatcgcaac cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat
    23641 tctttgacac ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca
    23701 ggaacaggcc ccttgctgtt ttttggatag tagatgtcat aagtattctc tttatacttg
    23761 gactcatacg tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt
    23821 ttatcagtga tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg
    23881 ggggaaagct ggaaggcaag aaagattgca aacgcgatcc caagaatgat tccgaagaac
    23941 catccgataa atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc
    24001 attctgatta taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt
    24061 ccccaaactt cttctgcaat gcttttgaca aaagaaattt tttccattgt tcttcaaaca
    24121 aaacattttc ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca
    24181 aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt
    24241 cgccattttt cgttacagtc aaaatctaag acaactaatt tatcttcaga gactttctct
    24301 aaaagaacaa aactcccagc acgttcacta tcgtattcta aataataggc acggacattt
    24361 tcttgagtaa aataatgggt ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa
    24421 taataatttc cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata
    24481 aaaatctttt tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg
    24541 acgttcattc tgacccagat ttgattttgc acaactcaac agtccttatt ggcaaatcgc
    24601 caaaataatg tagactaact aaaaagacta agaaacaata acaaaagtta acacttctta
    24661 gtctttcttt agcacgtcta tttattaaag attttagggc cttttctcat gtttttacga
    24721 tcattcttag gtgtttccat ttgtttggat cggcctccac cttgtttctt tttgatcaat
    24781 tctttcattt tttctttttg gtccattttt attctccttt aacgaatgaa gcgactaatt
    24841 cgatatgatg ggtttgcggg aaaagatcta caggctgaac tttcttcacg gcataacctg
    24901 cttctgcaaa taacttagca tctcttgcaa aagttgccgg attacatgag atatagacga
    24961 ttcgttctgg tgacatttcg attgctgatt caataaaatg atgatctaat ccttttcgtg
    25021 gagggtcaac tacgatcaca gatggtttga tccctttttc ttgccatttt ttcataactt
    25081 tttctgcttt tccgacttca taatgaacat tttcgatttt attatttaaa gcattgaact
    25141 gtgcatcttc aattgcttct ggaataactt ccatcccata caccttatcc actctgtctg
    25201 ctaaagacaa gccaatcgta cctattcctg aataagcgtc gatcacaatg tcttttttat
    25261 ctaactcggc aaactcgatg gctgtttggt acagatactc tgcttgttga gtatttactt
    25321 gataaaatga ctttgctgag attcgatagg tttttcctaa tagctgatct tcgatatagt
    25381 ctctgccata taaaactttt tctttgtctc ctaaaatcac atttgttttt tcttcattga
    25441 tattttggat gaccgaaacc acttctggca attcttcatg aatagcttca gcaatttcct
    25501 tggatttaaa gaatttttct ttacgagtaa ctaaaacgac catcatttca tgggaataat
    25561 gaccacgacg gataatgatg tgacgaagct gtccttcatt tttttcttca ttatatcctc
    25621 gtacttggaa ccgttgcaag atatctctga ctactttgat tgcttgatcg atgacaggat
    25681 cttggatata aaaatcttcg atagggacta gatcatggct attctttttg taaaaaccgg
    25741 tggctaactg tccatcgata cggcgaacag ggatctgtgc tttattacgg tagccgaatg
    25801 gttctgccat tccgaccgtt ggaagtacag gaacttctgg catcttagca atcttcgcca
    25861 ttacatgctc tacttgtttt tgtttgaaga caagctgttg ttcatacttc aaatgggcta
    25921 aaggtgcgat acctatgcga agtaatgcat tgttttctac aggctgacga tcaggtgatt
    25981 gtgtaataaa tgtttcgact tttccatatc caaacttatt gcctgctttt actactagta
    26041 tttctacttc ttctccaggg agtgcatttt cgataaataa tggataacca tcgatttttg
    26101 ctacccccat tccttcataa ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct
    26161 ttactatttg ctgttctgtc atattttgct cctttaagcc tatttttcaa tcctactgaa
    26221 ctctactata aatcagctaa ttacacaact aaaaccggca ctgaccttac tacgttcagc
    26281 accggctttt gattaattct tttgattagg tgtttcgtct gctatttttc cgtcaccgtc
    26341 gatatcttca tccgttaaac gttcgacttc ttttacgaat tctttgctga caacctctaa
    26401 ttcttcttca tctgtacctg tgattgcttc atcaggaatc tgatcggtat tggcaaagaa
    26461 ctcaatgtgt tgatgaagat tttggaaatg catcggtgct tccccgccgt attcaccatc
    26521 taagttgatc attactggtc gattgatatc atcttctgtt tcaacgaaca aactgcttgt
    26581 tttggtatag attattcttg gatcatcgac atgtcgaccg ccattcagca tgagtgctgc
    26641 aatatgcaag atctcaaaga tatttgctgt cttcacaatg attaaagaaa acttcccatc
    26701 atccaatttg gcatctggcg cgatctgttc gaatcctcca acagagttcg tcagccctag
    26761 gaaaaacatc gaagcatttc cgatatattc cccttcatca tacttcaagc gcattttgat
    26821 tggctttact cgagggagca tctctgctcc tttcgcaaga taagcaagat aaccaaaaat
    26881 actctttagt tctgatggaa cttcataagt caattctgtc aaatagccac ctgcagcgat
    26941 attgatgaag taactgttat cagaaaggcc gatgtccatt tttacggttt ggtgtttttt
    27001 tatcacttct gctgctgctt taatattatc tctaggaatt tttaatgctc gagcatagtc
    27061 gttcgttgta ccagctggga tgatggccat tttaggacgg tgttcaagcg gagcgattcc
    27121 attaaccact tcgttgattg tgccatcgcc accagctgcc actactagat caaaacctag
    27181 ttctgcgact ctacatgctt cattcttggc tgaatctggt tctggtgtag ttgcataggc
    27241 gctggcttca aatcctgctt cttctaatgc ctgtaggata tccgccaaat tttttttcaa
    27301 taattctttt cccgaggtgg ggttataaat tacgcgtgct tttttcattt tcagcctcta
    27361 tcgtttcgct aattcatctt taagtagctt attcacgaca ccaggatttg cttgtccttt
    27421 agttgctttc atgatttgac cgactaggaa gccaaccgca cgatcttttc catttttgaa
    27481 atcatcgaca gattgttggt tgttatccaa aatttcatta atgattggca gtagtttgtt
    27541 cggatcagat aattgcaccc aaccattttt ttcaactact tcttgggctt ctccgccatt
    27601 tgtgatcaat tcacggaaga cttttttggc gatttttgag ctgatcgttc catcggcgat
    27661 caaacgaatc attcctgcta agttctcagg tgtcaatttt gtctcagcta attccagttt
    27721 ctcactgttc aagtaagcag aaacttcacc catcaaccag ttagatgctt gcttggcatc
    27781 tgcaccattt tccaaaacag cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac
    27841 cattgcgtca tactctggaa gtccaaactc acggatatag cgttcgcgac gagaagctgg
    27901 catctcaggc aaactagctt ttactttttg aatccattcg tcatcgatca cgaatttcgg
    27961 gatatcaggc tctgggaagt aacgataatc actagaccct tctttgacac gcatcaaaac
    28021 tgttttattc gttgtttcgt caaaacggcg tgtttcttgt tggatttctc ctcctgaaag
    28081 aagaactttt gcttgacgtt tttcttcaaa agctaaacct tttttcacga aagataaaga
    28141 gttcaagttt ttcaattctg ctttcgtacc gaactcttct tgtccataag ggcgaagaga
    28201 aatattggca tcacagcgca tagagccttc ttccattttc acatcgctga cttctgtaaa
    28261 ttggataatt gatcgcaatg cttctagata agcgtatgct tcttctggtg aacgcatatc
    28321 agcttcagaa acgatttcga tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata
    28381 accgccaata ccatgcatgt tttttccagc atcttcttct aaatggacac gttcgatccg
    28441 gatttttttc tttttacctt ctacttcgat ttcgatccat ccgtcatgac cgattggttg
    28501 atcaaattgg gaaatttgat atgcttttgg attatctggg taaaagtaat ttttccggtc
    28561 aaaatgagta tctttagaga tttcacagtt taatgccaaa gctgctttca tcccgaattc
    28621 cagcgcagct ttattcatta ctggcagcac gcctggataa ccccagtcga tcacgtttgt
    28681 attgctgttt ggttctgcac caaaatgagc tggcgctgga gagaaaattt ttgagttggt
    28741 ttttaactcg acgtggactt ctagtccaat gactgtttca aaattcattc gtctttccct
    28801 cctaaaatag ctggttgttg tttatggaat tctgttgctt gttcaaacgc atatgccgct
    28861 tgatacatcg ttttttcatc aaaataatta ccgatgatct gcaatccaac tggtaatcct
    28921 tctgataaac ctgccggtac agacatacct ggaagtccag ccaagttgac agggatcgtt
    28981 aagatgtcac tcatgtacat tgtgattgga tcgttaatgt tctcaccgat tccgaatgca
    29041 actgttggtg aagatggccc gatgatcaag tcataatctg caaagacttt ttcaaaatct
    29101 cgtttgatca atgtacggac ttgtcctgct tttttgaagt aagcgtcata ataacccgcg
    29161 cttaaggaga atgtacctaa catgatacgg cgttttactt cttcaccgaa tccttctgaa
    29221 cgtgagttga catatacatc atctaatgat ttcacatttt cagagcgata accgtagcgg
    29281 ataccatcga atcgttgcaa attggaactt gcttctgatg aggcaatgat ataataaaca
    29341 gccacaccgt attttgaatg aggcaagctg acttcttcca caacagcacc taatgaacgg
    29401 aatgtttctg ctgctttcaa tacagcttct tttacgccag gggccacacc ttcgcctaag
    29461 tattctttag gtaaagcaat cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa
    29521 tcaggaactg aaacgcctga agaagtaccg tctttttcgt cataaccgct gatagctgtc
    29581 aatgctaatg cattatcttt caccgtacgg gtcattggtc cgatttggtc aagagatgaa
    29641 gcaaaggcaa tcaaaccaaa acgtgagaca cggccataag taggtttcat ccctacgatg
    29701 ccgttaaaag ctgctggttg gcggatactt cccccagtat cactacctaa agaaactgga
    29761 atttgtccag ctgcaactgc tgctgcagaa ccaccagaag agccaccagg tacttttgtt
    29821 gggtcccaag cattttttgt tttcttgaaa taagaagttt ctgtacttcc acccatagca
    29881 aattcatcca tgtttagctt accaacaggg atcatatctg cttgatagat tttatccatg
    29941 accgttgcat cataaattgg ttcaaaattg taaaggatct tagatgcagc ggttgtcaag
    30001 atatcttttg ttacgatatt atccttgata ccaattggaa taccagcaag gacgttgctt
    30061 tcatcaatcc ctttttcatc taaagctttt gcctgttcca atgctttttc ttcgttcaat
    30121 gtaatgaatg cctcgacctc aggctctgtt tctttgattc gttcaaatgt tgtttttgtc
    30181 aaatcttgta cagtgatttc tttcgataca agcatatgat gcagttcttc tagagaacga
    30241 ttgtgtaatt ctgtcattat gcgccagcct ccccattatc gatgattgcg ggtactttga
    30301 taaagccgtc ttctgtttcc ggcacatttt tcaataattc atcgcggttc catccagctt
    30361 gcgcccgatc ttgtcgcatc acgtttactg tttcaaccac attcgacgta aatggaacac
    30421 cttccgtatc tacttcttcc agcatttcaa ccatatcgat gattttacct aattggtcgg
    30481 taaaacttgc aagttcttca tctgcgaacg caagttttgc taatttggca acgtgtttca
    30541 cttgttcttc actaatcgcc atactacccc ttctttctaa acgagttaaa aactttgctc
    30601 tctattttaa cacaagagtg ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac
    30661 tcatttttca ttcttctact gagcgacttt tagccaatgc atctgccatt tgatcttctg
    30721 tccaaacttc gattcctaat tcttgtgctt tggtcagctt gctgccagca tcttctcctg
    30781 ctaccacgat atccgttttt ttcgatacgc taccagtcac tttcccgcca agattttcga
    30841 tcgtctcttt tgcttcttca cgagtaaatc gtgttagttt accagtaagg actactgttt
    30901 tttctttaaa aggagactcg acttcttgca attgggcatt tcgaagccct ttgtattcga
    30961 aattgacgcc tgcttgtttt aattcattca tcaattcatg gacttcttcg ttactgaaat
    31021 aagtgactac actgtcggca atgatatcac cgatcgtatc aagcgcaata atcgactcat
    31081 agtcactttt actcaatgtt tctagatcac cgaaatgttc tgctaaaatt tttgctgctt
    31141 tcgctccgac atgtcggatt cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc
    31201 tgttgtcgat tgctgtcaaa atattatttg ctgatttttc ttttattttg tccaacgtca
    31261 ataactcttc ttctgtcaat ttgtaaagat cagcaacatc tgctactagt tttttatcat
    31321 acatctgttc tagtacgcgc ggccccaacc catcgatatt catcgcattt cttgaaacaa
    31381 agtggttcaa accttctttc atttgagcag ggcatttagg attgatgcac cgtaaggcta
    31441 cttcttcatc caaatggaca agttcacttc cacaaactgg acaatgtgta ggcaattggt
    31501 attcttcgct gtctttaggt cgtttatcta acacgacttg cgaaacttct gggatgatgt
    31561 ctcccgcttt ataaatcaga actgtatctt tcaagcggat atccttcaac ttaatataat
    31621 caccgttatg caggctggca cggcttactg tcgttcctgc tactcggact ggtgtcatga
    31681 tagctgttgg cgttactacc cctgttcgac cgactgtcca ttcgatgttt tcgattagcg
    31741 tttcaacttc ttctggcgga aacttgtaag ctgctgccca tctcggtgct ttgatagtaa
    31801 aaccaagttg atcttgtagc gagaattcat tcactttgat gacgattcca tcgatttcat
    31861 agggcaaatc cacacgtttg tcatgatatt cttcaatgta tgaccacact tcatcaatcg
    31921 aatgacatag acgtttttcc cgattcgtgt ggaatccaat tttttctagc tcttctagcg
    31981 catcatactg ggtttttgct tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt
    32041 ccagatttct tttcgctgtg attttagaat ctaactgacg caaagaacca gctgcggcgt
    32101 tccgaggatt cgcaaagata tcttttccct ctgcttctct gtcttggttc aattggacaa
    32161 atgaccgttt tggcatgaaa cattcgcctc ttacttcgat gttcattggt tcttttaatt
    32221 tgattgggac cgaccggaca gtttttaggt tttcagtgat gttttctcct acagttccat
    32281 ccccgcgagt cgcacctctc acgaaatttc catcttcata acgcaaagaa acggaaagtc
    32341 catctatttt tagttcacag caatagtcaa ctacacgtcc cactgctttt tgtacacgtt
    32401 gatcaaaagc tatcagctct tctttgctga aaacgtcgtt caaactgtaa agcggaatat
    32461 catgagtgac tttttcaaaa ccttctaata cttttccgcc tactcgttga gtgggagaat
    32521 cagacgtgat caaatcagga tattgttcct cgattgctac taattctgcg tattctttat
    32581 cataaacata gtcttccact gttggtttat cttctacgta gtattcgcga gaccattggt
    32641 tcagtcttgt acgaagttct tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt
    32701 ccatcattta ccctccacta ctaagctttt tcaattggcg caaaggcagc caaaagacgt
    32761 tttactcctt gacttgggaa agccacatct agttctagat ccttcgacgt cccgcttact
    32821 cggacgactg ttcctagacc ccattttttg tgtttgactt tatctccagg tttccagctg
    32881 ttattttccc ctccagtagc acttttagag ggaacaaccg ctgtctctgg ctgtttatag
    32941 ctatatgctg tttttttagc gaatacaggc gtttttttag gcgtaggacg catgccttcg
    33001 atttccagta actcctgatc gatttcttct acaaaacgag aaggtcggtt atattgcgtc
    33061 cgtccatata atgttctgga gaaagcattt gttaaataca acgcttcttc tgctcgcgtg
    33121 atccccacat atgcgagacg gcgttcttcc tccagttcgc tttcttccat caatgctcga
    33181 gataaaggaa aaaccccctc ttctagacca atcaggaata caactggaaa ttctaatcct
    33241 ttggctgcat gcaaagtcat caatgtcact tgggaagcat cttcttctaa attgtcaatg
    33301 tcggaaacaa gtgccagatc atttaagaag accgttagtt tttcttctgg tgcatcagcg
    33361 tcttcttcat tttgctgttc gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc
    33421 tccaagtttt cgatgcgtgc ttgtgcttct aatgtatttt ggatttttaa gtcttctaga
    33481 tatccgctac gatccaaaac ctctttcgtc aattctgtca ctgtcaagta tgggatcatc
    33541 tgagtgactt cttggatcat ctctccaaat gcacctaatt gctgtcctgc ttttccaccg
    33601 atatttgcca gatccacatt ttgcgctgct tcaagcagtg gccattcatg aagagaagca
    33661 aaactgcgta gtttttcgat agatcctggt ccgatgcctc gtttgggcga attaacgata
    33721 cgctcaaaac tgattgaatc ttgaggattt accagcacat tcaaatacgc taaaatatct
    33781 ttgatttctt tacgatcgta gaacttgtgc cctccaacca ttttataagg aatatttgct
    33841 ttcaacagag tttcttccat tacacgagat tgtgcgttcg tccggtacaa gatggcaaag
    33901 tcgccgtaat tccgatgatt gcttcgaatc tcttcttgca tccggtctac gataaaacgg
    33961 gtttcgtctc gttcattatc tgcacggtag taagtgatct tgtttccttc tttgttttct
    34021 gtccacaagt tttttggttt tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta
    34081 aggatgtttt tcgtggagcg atagttttgt tctagcaaaa ttactgcagc atctggataa
    34141 tctttttcaa aatccaagat gttctgcata tctgcaccgc gccagccgta aatactttga
    34201 tctgcatctc ccacgacgca caggttccta aatctacctg ccaataaatt gactagcgta
    34261 tactgtgcgt gatttgtatc ttggtactca tctacatgaa tataatggaa tttgttttga
    34321 taatacgtga gtgaatcagg atgttcttca aataatcgaa tcgtattcat gatcaggtcg
    34381 tcaaagtcca tacattgatt atattgaagc tctttttggt aagcagcata acattttgcc
    34441 gcaatctctt cgtaataact gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg
    34501 ttttttgctt gagagattgt tcctaatata cttcgaggat catatttttt cggatcaatg
    34561 ttcagttctt ttagtatccg tttcatcaag gtcagctgtt cagaagaatc gatgattgta
    34621 aagttccggt tatacccgat aaaatcaaca tctcttctta aaatacgcac acacatcgag
    34681 tgaaaagtcg atacccagac atcttcgcct ccagatgcga gaatcgcatt gacacgttct
    34741 ttcatttctt ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca aggattgact
    34801 tctttttcct caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct
    34861 gcacctgcca ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg
    34921 ttcatcccgt ttaataattc agctttggat gacattaaca acatcctttc ttttatcaat
    34981 cttgttaatt atagcatttt cttggctttc attaaactag ctagttcaaa atcatttgtt
    35041 tctttttatg gatttttcct tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga
    35101 ggcgtttgaa tggaaaaaaa aggaaaaatc aagaaattag ataagactca gatatatttt
    35161 attctaaaag gaatatttgt tggactgctg acaggaatcg tcgttagcct gtttcgatta
    35221 tctatcgaaa agctaagcga tttagttcga agtatctatg aaatgagtcg ggaacagccg
    35281 atttacttaa ttggcctagc tatttgctgc atccttgcag catttttcgt gggttatctg
    35341 gtcaaaaatg aaccggatat caagggaagc gggattccac aagtagtggg acagcttcgc
    35401 ggggaattat caatgaactg gttctctgtt ttatggaaaa agttcattgg cggtgtactt
    35461 tcagttggtg cgggcctttt tctaggtaga gaaggacctt ctatacagct tggcgcatcc
    35521 attggacaag gagctggaca attgtttcgg agtccatctt cagaagaaaa gattttaatt
    35581 tctagcggtg caagtagcgg acttgcagca gcattcaatg ctcctatcgc aggattactc
    35641 tttgttttag aagaaatcca tcactccttt tctccacttg tttggctgac atcatttgct
    35701 tcagcaatca ctgctaactt cgtctctctt tactttttcg gcttgagacc tgttttatat
    35761 ctaggtgacc ttccttcttt gccgttgaaa tattatggtt ccttagtgat cttaggagta
    35821 cttctaggca tcttaggttt tatttatcag aatgtcttgc tggctttgcc aaaattttac
    35881 aaccgattgc ctttaccagc ttattttcac gggttcgttc ctttcttgct gattttgcca
    35941 attggttatt tatggcctag tactctaggc ggaggcaatc aaatcgtttt agcttttgga
    36001 catgcttcct tgccaatcat tatgattatc ggtttgtttg ttcttcgatt cgttttttcc
    36061 atgatttctt acggtggggg tcttccaggg gggattttcc ttccgatatt gacattgggg
    36121 gctttaatcg gtagctttta tggaaacatt atggttgatt tcgggatgga tccaatctac
    36181 atcaaggatt ttgtcgttat tgccatggct ggttatttca cagcgattgg aaaagcaccg
    36241 ctgacagcaa ttattttagt gacagaaatg gttggctctc ttaatcatct gatgccttta
    36301 ggattggctg ctcttgttgc ttatatcgtc aatgaccttc tcggcggtaa tccaatttac
    36361 gaatcattat tggaacgtct acttggaaat actcagaccg cctcactaaa aggtgcaagg
    36421 acaacttttg attttccagt aacggcagag agttctttag acggattaat ggtacgagac
    36481 ttcaattggc ctaaaaatat gctgctaatc tctatacgca gaggaaatca ggaaatcttg
    36541 actcacggtg atacgatcat gaatgtagga gatattttgg ttatcttgac tgatgaagca
    36601 aacttgccaa gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa
    36661 tcattttaac gaacatggaa aaactatttt acaacaacta ttcatctaca ctacttgatt
    36721 ctatatgatt ctataaaaaa agaagaggct gggaaaaccc agcctcttct tttttatttc
    36781 tatgcttctg ctactgcagg agcttttctc aataaaccat aaatcacacc agatacgact
    36841 gctccgatag caataaacac caaatacatg accgggtgac tcagtaagaa tacaacgaaa
    36901 atcccgccgt gaggagccag taatttgatt ccgaaagctc cgactaatcc gccagttaaa
    36961 gcagaaccaa taataaaact tggaatggca cgaatcgggt cagctgctgc aaatggaatc
    37021 gatccttccg tgacaaacga tagtcccatc acgatgttcg tcaaaccagc atcttgatct
    37081 gttttgctga atttattctt aaacaaacgt gtagcaacga aaattgccaa tggaggtacc
    37141 attcctccag ccataacaga agccatcacg acacttccgc cagttgcaac agtcgtagct
    37201 aaagttgctg taccaaacac ataagcggct ttgttgattg gaccgcctaa atctgccgcc
    37261 atcattccag ctaataatgc accaagtaaa gcagcattcg aaccactcat acttgtcaag
    37321 aaaccgttca atgcatcatt gattgctttc atagggacat tgaccagcaa catcaagaaa
    37381 ccagtaatca gtaagccaaa cactggataa aagaggatcg ttttaattcc atccaatgat
    37441 tttggtaagt tcttgaataa tttacgtaaa aatacgatga cataaccagc taagaatcca
    37501 ccaatcagtg cacctaagaa tcctgcaccg cctgtattag ctaatgcacc agcagcaaat
    37561 ccaacgatca aaccagggcg gtcaccgata cttgaagcaa taaatcctgc taataccggc
    37621 agcatgaaac caaatgcagc ttgtccgatt tggttgaacc agctagcagc ttgattgtaa
    37681 ttccctagat ttgataattg atcatgtggc acgccaataa attggtcgat catgaaagaa
    37741 agcgcaatag cgattcctcc accaataacg aaaggtaaca tgtgtgatac gccgttcatc
    37801 aaatctttgt aaattctttg tccgatagta ccatccgcat ttccttcttc tgaactgctt
    37861 tcttttccat caccatggaa tactggtgca gtaccactaa gggctagatt gatcaactct
    37921 tctggtttac ggataccatc actgacagga cgattgatca gttcttttcc gtcaaagcga
    37981 ttcatctcta cttttttgtc tgctgcaacg attacacctt ctgcacgggc gatgtcttca
    38041 gcagtcaatc ggtttttgat tccttctgaa ccgttcgttt cgactttgat ctccacaccc
    38101 atttctttcg cttttttctt caaagcatct tctgccatgt aagtgtgagc gatccctgtt
    38161 ggacaagctg taacggcaac aacgaatttt ctttctggat gagcagaagc ttctgcagct
    38221 tgtgtttctt gttctgcttt ttcttctgct tctttttgct cttcagctgt ttggaacaag
    38281 tcttggactt cttctggtgt atttgcttct tttaattttc ctacgaaatc tgcatcaatc
    38341 agcaatcgag atagtgctgc taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct
    38401 gcgatcatga aaaacagata agttggctgt ccgtctaagg cttcataatc tactcctttt
    38461 tggctttttg cgaataaaac tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg
    38521 gcgattccat caccaagacc tgtagaagtt tgtgcttcgc gggctagaat tccttcttta
    38581 tatgtttcga tatcggaaat ccggccggca tcaaacattt tttggaccat ttcatcgatt
    38641 gcgccttttt tatcagtcgc ttgcaaatcc atgatcatcg cttctttgac cattaaatcc
    38701 ttgatattca tgattatcct ccttgaaact aatcaatttt tgtgatttca acttctggca
    38761 gcaactcatc aatcaattcg cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc
    38821 aagcaactcc ccatttaaag gcttcgattg gatctttcgt acggctgtaa gccccaataa
    38881 agccagcaat cattgaatcc cctgcaccta ctgaattttt caatgggcga actaatacat
    38941 tcgttttata agttccttgt tccgtgaaga gcaaagcacc gtctccagcc attgagatca
    39001 gcacattctg tgccccttca gctagtaatt ttttcccata cggaatgatt tcttctacag
    39061 attggaaggt tgtgttgtaa agctcagcta attcgtgatt gtttggtttg atcaacaacg
    39121 gtttcttaac aagtgcattt tttaggtcat cacctgttgt atcaatcacg aaatctgcac
    39181 ctttttctcg aatgatatca atcagttctt gataaaatcc ttttcgcagg ctagctggcg
    39241 cgcttcctga caatacgacg atatctcctt gtcctactgt tcctaatgct tgcttcaact
    39301 catcgatttc ttcatctgaa atagatgggc ctaagccatt gatctcagtt tcagtgtctg
    39361 attttagttt gatattgatt cttgtatctt cttttacggt tgtgaacaaa gactggattt
    39421 gttctttttc aagccacttc gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg
    39481 ctgtagattg agaatcgatc cgtttcaaaa tacgggaaac attgatccct tttcctccag
    39541 gtaacttgaa gtcttccttc attcggttca aatcgcctag ttttaaccca tctacgcgta
    39601 cgatataatc aattgatgga ttaagtgtta ctgtgtaaat cattgtattg cctccttaat
    39661 tgttgtcttt tcacggaatt cattaacgaa ttctaatgga caacgctcgg ttatgatcgt
    39721 ggctgcttcc aaaggtgcaa cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat
    39781 caatacaaat gtctcttcgg aatttttcat ggctaaacgt ttaacagctg cctcttctgg
    39841 atccggagtc gtgaatcctt gttcaagatg ggcaccgttc attcccataa atgctttatt
    39901 aaaatgaaaa ttttttagtt gttctatcgc attagatcct aaaacagcat tagttgccag
    39961 ctttatcgtt ccacctaaaa taatggtagc aagctccaaa tcaactaaat ttgccgcatg
    40021 ttgaacggaa tttgtcacta ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa
    40081 cgtagtagag ccagcgtcta aataaatgac atcttcgtca ttaatggttt cagctgccga
    40141 tttggcgatt tgctgttttt cgtgaacgtt tttgagtgat ttttcactca tattctgctc
    40201 aaaattcaat aattgctctt tcttagctcc gccatgaact ctttctagaa acccactatt
    40261 ctccagctct tgtaaatcac gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag
    40321 atcttgtgat ttcacgattt tctgctgttc aagcaagtct aggatttttt gtctgcgttc
    40381 ctctgtaagc attttcacga tcacctcaat acaaataata gcatattttt tttctactag
    40441 caatataaat cagtcaaaaa cgttcataaa aattataaat cagtcaaaaa aaaataaaaa
    40501 accagtcaga aagactggtt ttttattttt ttgtctatca tcaagccgtt ttatcgtttt
    40561 ttattttcgc ttcttgtttt tttcatataa acagaagcag gttttctttc cttatgaaga
    40621 agataataat aaagtcccat cccgccaaac atgaccaaaa aaccgatgac tgatcgaata
    40681 ggaaaattag gaacaaaaaa gagacagacg gataataatg atgccggaac cattattcga
    40741 taatagagtt gtttatccat tttcttcacc tcatattttt tgtaaagtaa tcaattgttc
    40801 taattctcgg taatctaaaa attgcacacg cagatgctgc tcacctttct tgacatcgaa
    40861 ttggatactt cgccgctttt tctttattcg tttgaaggta acatcagaga gtctatgttt
    40921 gatttccttt aacggtaatt ctgacaggga ttctgaagga acatagattc ttaaggataa
    40981 aagatactct tgatattttt catctgttac ctctacttct agatattccc taatataata
    41041 acaataagct tcgattccat tatccaaagc aacagtcgga tcgataactt tatagcacat
    41101 catgcgtgat cgattctcgt caacccaagg aaaatacgcc tgagcattta gtgttccgcg
    41161 aacacgcctt tctttataat ctccttgttg gatatcataa ctttttgtta caaaaaatga
    41221 agcattcttt ttaggaacag gttgcatttg tgcattctga tactggacag attggatcgt
    41281 atggtcagtg taaatgtatt caacgaatgg cggtttcaat aaaccgacac cattgtccca
    41341 agcaagatga tcaaacccaa gctggtccct aaaataggat tgaaacattt gattgtttgg
    41401 cttttgccag ttaaaggggt ctcgataaag ctggatacat atgacttcgt tttctaattc
    41461 cttcactttt tctacttgtt ggatgtcgag aaaaatcggt ttagggatga tttgatgata
    41521 atagcgacta taatacattt cccaacacga agtgaaacat aaaccttggt aaaaaagatc
    41581 gtatcctgga aattggttgc aatcgactac tacttccccg ttttgttgtt tcattttagg
    41641 taaatgttcg atttcatcct ttttctcaaa caatagccgt tgagagatct gttttgtatt
    41701 gtgatagata aactcgctat aagctcgcat ataggcgaac accccatgag caatcatttt
    41761 tttctgaatg aagtctcgaa tcaaaaattg cttttctttg aaaacagaga tagacaatac
    41821 attttttatc aataattgat tatcttccaa gcgaaaacta cattcgttct cgtcatttcc
    41881 tacaactaac caactttcgt ctcttaccga tgctagcatt attttttctt gaagctttcg
    41941 aagatcatac ggatccgctt gttgtgtgaa agagcggata aatgttggct ggatcaacag
    42001 acgtgtcaaa atagcccaat cgcttatttc ctgttttttt tcttcttcat ttaagtctgt
    42061 gagctggata gtcatcaata atttatcttc gccaatcatt ttctcacctc tttcaaatct
    42121 ttttataatt taattataac caatgataaa gaaaaactaa aactttatgt cttagaaacc
    42181 aatttggacg attacatgat agactaagta aaaagaaaca aagaggtgtt tacaatgggg
    42241 ttgaaatttg aacgtacaga taatttagat aaacttttcg aatcatttgc tgtcgatccg
    42301 gaaaaaacag ttgcaccaaa agaagagatc gatcgatttc taaaaccaca gccacaaaat
    42361 gaaaagaaaa aagacaagaa acaaaagtaa gttaagctca aaaaaagact taccagccaa
    42421 acttttatga aagagtttga ctggtaagtc ttttttattc tttttatgat tcaattgctt
    42481 gttcgattgc tgcccacgct tcttcttttc cctgttttgt aacggaagaa aagagaataa
    42541 acgtgtcatt gcgatcaaaa tccaacgctt ttttgatttc gctctcatgc ttgttccatt
    42601 taccacgagg gatcttatct gccttagtgg caacaacaat tacagggata tcataatact
    42661 tcaaaaattg atacatctgg atatcttctt gagaaggctt atgacgcaaa tctactaaag
    42721 aaacgaccgc acgtagctgg tctctagtcg ttaaatatgt ctcgatcatc tttccccatt
    42781 ttgctcgctc agttttggat acttttgcat acccatatcc cggaacatcg acaaaatgta
    42841 atgcgttctc gatcaaataa aaattcaatg tctgggtctt tcctggctta ccggaagtac
    42901 gggctaaatt ctttcgatca atcaatgtgt tgataaaaga agatttccct acatttgatc
    42961 gtcctgccag agctatttct ggcaattgtg tatctggata ttgttttggt gccactgcac
    43021 ttatgataat ttcagcttga tggactttca tggtctctct ccttaacaag gtgatgaata
    43081 aagcaagcgg ctgtcgcaaa agccaaaatg acttttacgg cagcctcttt tcatcaaccc
    43141 gcttttttct ctgaatcttt atagattact gttggctcac cagacaattc agctgcttct
    43201 tttgtgataa tcactttttc gatgttttca tttgaaggga tatcaaacat cacatccatc
    43261 atgatttctt caatgatgga acgaagtcct cgagcaccgg tattgcgttc gattgctttt
    43321 ttcgcaatgg ctctcaacgc ttcaggttcg aattcaagtt cggtattatc tagagaaaga
    43381 agtttttgat attgtttgac tagcgcattt ttcggctcag tcaagatccg taccagatca
    43441 tctgttgtca atttctctaa agctgccata acaggtaatc gtccgataaa ctcagggatc
    43501 aatccgaatt ttagtaaatc ttcaggaatg atatgctgca tgacactttc gtcttcagaa
    43561 agtttttggt tttgtgtacc aaatccaatc gtcttttcgc ccatacgatt cttcacaatc
    43621 gtttcgatgc catcaaatgc accacctacg atgaacaata tgttcgtcgt atcaatctga
    43681 ataaattctt gatgtggatg tttacgtccg ccctgaggtg gcacacttgc aacagttcct
    43741 tctagaattt tcagcaaggc ttgttggaca ccttcgccgg aaacgtcacg agtaatagat
    43801 acattttcgc ttttacgcgc aattttatcg atctcatcaa tatagatgat ccctttttct
    43861 gcaagttcca cattgtaatc agcagattga agcaatttaa gcaaaatgtt ttctacatct
    43921 tcccccacat aaccagcttc tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc
    43981 aatgtttttg ctaatgtttg tgctaaaaat gttttaccag accctgtagg tccgatcaag
    44041 cagatattgc ttttttgcag ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca
    44101 cgtttatagt gattgtatac ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc
    44161 acgtagttat tcaatacttc aagaatttct agtggtttag ggacttcagt gaattcgcgt
    44221 acagcttctt cataaaattc ttcatcaatg atttctttac ataggtcgat acattcatta
    44281 cagatgtaga cccctggacc agctacgatc tttttcactt cttcttgggt tttcccgcaa
    44341 aaagaacagc gtacggtctc attgctactt ggattgtcat acatggtctt caccccttaa
    44401 cttgcaaaaa gtatgcaaga aactatcaaa gactttacag tcttttacta tcataacata
    44461 gtttagtaaa aagaaaaagt gtttaacaaa ggatcaactg acctatacct ttgcattttt
    44521 tactcttaac aaaaaataag gaactgtgac aaaatcgtgt cacagtccct tatttcgaat
    44581 aaacaatttt ctagaagtag cttcttcaga aatgatttat tacttgattt ctattctttc
    44641 gcttctgtcg ctgtacctgt gatcaattcg attgcttttt tcattgtcac atcatgttcc
    44701 agcatatctt ctgtcaacac gcgtttgatt tgttcgattg gcatgttgta tgtttctgat
    44761 aattcttgga tttctgcatt gatttcttct tctgttgctt gaagattttc agcagctgca
    44821 attgcttcga tcacaagatt tgttcttgtg cgcatttctg cttcaccttc gaattgttta
    44881 tgcaagtctt cttctgtaga accagttaat tgatagtaca tttcaggaga gataccttga
    44941 cgttgcatgt tgttcaagaa ttcgtccatt gaacgatgta cttcatcatg aaccatcacg
    45001 tgtggcaatt caacgatttc tgcgttttca acagccatac ggatcgctgc ttcatctttt
    45061 gcatcgtccg ctgctttttc ttttgtttct actaattctt tgcgatattt ttctttcaat
    45121 tcgtctaatg ttgatacttc gtcatcaaca tcttttgcga attcgtcatc taattcaggc
    45181 acttcttttg cttttacttc atggattgtc actttgaaga ctgcttcttt tccagcaaga
    45241 tcttctgctt gataatcttc tgggaaagtt acatttacat ctagactttc acctgattta
    45301 tgaccaacta attgatcttc aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag
    45361 taattttcgc cttttccgcc atcaaacgct tcgccatcta caaagccttc gaagtcgatt
    45421 actactgtat cgccattttc agctgcagca tcttctttga taactaattc agcttgtgct
    45481 tcttgttcac gtttgatacg tgcatccacg tcagcatctg ttacttcacg gtcttgtttt
    45541 tcaacaacta gatctttata ttgtcctaat tttacttctg gtttcactgt tacttcagca
    45601 gtgattaccc agtcttgacc tttttccatg ctttcaacat cgatttttgg ttgagaaaca
    45661 ggatcgatgc cagcttcttt gacagcagct tcataagctt ctggtaatac agcattcaat
    45721 gcatcttcat ataaagcttc ttcaccgtac atacggttga atacttgacg agaaactttc
    45781 cctttacgga atccaggtac gttcaagctt tttttcactt tattaaacgc agttgttaat
    45841 cctttttgga tttcgtcttg cgcaatcgta aattttaaaa caccatcatt cgtgcctttt
    45901 ttttcaaatg ttgcagtcat ttaattccct ccgagtttag aaattcattt tatacatcaa
    45961 aaacgtttca tgcatacctt taaatagtac actaaccctt tcaaattgta aacatattga
    46021 atggatagaa tccttgtttt tcataacatt tttatctttt tcactgataa aatctaatgg
    46081 aagaagatca tttgctgcaa aattttttct ctttttttta aaaaatcttg tttttcatct
    46141 tctgtacctt catacatcgt ttttcctgta cacgcctcta cagtcagttg aaaccagcgc
    46201 ttcgggtctt cgatgtatgt atcatagaca ggataaacaa ttgcagattg caacgtgaat
    46261 tcttctttta acacggaact aagcagcgca tcttcattcc cgtaacgatc tgaaaataca
    46321 cataattgct ggtaacttgc ctgctgctgc ggctttggca gatcttttgg tatgactttg
    46381 actaactttt cttcaatcgt caaatagaaa acttgatggc attcgttcaa ttgcctaagt
    46441 gtctccagta attttgccct tgctaaaata tggacctctg gcgatacaat aagtttactc
    46501 gctgtttgga taaactcatg ataaggaagc tgttcgatct catcaataga cgcaagctga
    46561 cgtgcaggtt ccaacgaagg aagttcctcc gatttattgt ggagtgcctt gatcttctgc
    46621 ctctccattt gtccggataa ttcttctgca aattggattt tttcaatgac ccgctgctcg
    46681 aaagcttcat aaaaatctgt acttgctatc aattttctag catataaaaa atcttgtgta
    46741 ctgattaaca agtcaaaata aaattcagca aactcctcat tttgaagata ctgtttttca
    46801 tgcaagagtg cttgcttcaa tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca
    46861 agcaggctat tagcctcaaa gctttctttc aactcgtaag ctcgttgaaa taattctttt
    46921 gcctgctgat attgctggtt tgctagtgct tgttgtgcca ctcgcattaa attctcatat
    46981 ttttctggaa aatcaatcgg cttcacgcag tgtcctcctc ttgctcatat tcagctcatg
    47041 ttcaatagta gcaaaacaaa aaaagcatga caattaaaaa taactgaagc accctctaaa
    47101 agctagattt tagatctgac ttttagaagg tgctttcaac tgttactgct ttttattgct
    47161 catcagctgt tgacttcctg gttgaacgct ttgatcagat tgatcgtaac aacacttgta
    47221 ttagtgaaaa aatcgcccag atgttgcttt ttctgattaa agactgctgg aagataccct
    47281 aacatcagca aaggaaattt tagaatgaac cggcaagcac cttctctaac taaaacggtt
    47341 gaccagctaa gttcttcttc atcaaagctg atgactcgaa taccaaagat cattttccca
    47401 atcgtttggc catgattgaa tttcgtcaat aaaataaaat acgctagata aataagtaag
    47461 gctgtgaatc cgtagagact cagataagaa tctgaaaaat taagccaacc tagatttttg
    47521 gcaataccaa gtgttaaacg cgtaatagct ccgatacaca aaagatcgac caagaaggca
    47581 aacattcgga taaaaaaacc tgcatagaaa tacttaggaa aatcatttgc ttttggcggt
    47641 tcatttcttg tgtccgtata ctttttccat ttacgttgtt tctttttgat ttcttcgggc
    47701 gtcaaaggct ctctttcaaa agagctcagt accttttggg aaaagttgga gtcaggtcct
    47761 acagcaggct gctgttgctt atctttttct tgttccttat tttcatttga aagctctgac
    47821 atgcttactc acctccgtaa taatacattg cttttggtga ttccgctgtc cctagattct
    47881 caagaacgga taagatctga ctagtctcag aaggcttcaa tccctggaat tctgctagtt
    47941 tacttcctaa ccatgtacta gcaaacccag atggcgtgga agaatattct actagttccg
    48001 catcttcaag ctgttgttcc tttcgtatag cttttaaagc atcttcagga aatcccaatt
    48061 catcgactaa acctacttct tttgcttgga caccatcata aatccttcca tcagcaattt
    48121 ttttgacttc ttcttcagat ttattccgtc cctcgctgac gattgctaca aaacgactat
    48181 atgcgctgtc gatatatgct tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc
    48241 ctgaacccat atctttcaac gctccgcttt taactgttgt atcttccaca cccagttttt
    48301 ctagaagtcc tgaataattc aatcctgaca tgatcacacc aattgagccg gtcactgttt
    48361 cttctgttgc aaagatttta tctgcttgag cagaaatata gtagccaccg ctagctgcca
    48421 tgtttttcat gctgacatac attgggatat catgttcttt acgaatcgta tccaatttct
    48481 tagcgatttc cgcactttcg tagacaccgc caccaggaga atttacttct aaaaagatac
    48541 ctttgactgt tggatcttct tcaatcgcct tcagttgttc cataaaattt ttatgattgt
    48601 aaccttcaga agcaaataaa cctgaagacc ctcccgcagc aatcgttcca tctactgata
    48661 attttacgat tttttgcgta ctgtctccct cttccaacac atttggagct agttcttcat
    48721 ccccatataa ccaactgttc aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac
    48781 tagctgaaac taacgaaacc acaaataatc ctgcagcaat cagaactgct gcccaacgtc
    48841 ttttattcat tctttctcct ttctaataca agttttttat gacgatcaca gtttctgact
    48901 gctcagaagt gtcgaaccgc tctgcttttt cttcccgcgc atacccggaa aacgactctt
    48961 ttgttaattc atcttctacc gtaaactcac gagcttgagc aacttccaag gcatcaggtg
    49021 cgatttcttg gatgctatcc cagccagtat ctaaaaatat tttcgtatca tctgatacac
    49081 cagctgatgc ttcgaatttg gacaattgtt tttttaacat tcccagtgtc atgatctcaa
    49141 tttttgaatc catttttact cctcctctat ccgattactt tctattgtac cgaactcctg
    49201 aaaaagatga tagataaaaa cccattattt tttgacataa aaaggacatt tgacaaagaa
    49261 tttgatatat accaatttat tttttattct ttttttgttg acaagaacat tcgcaaaagg
    49321 ttatgatttt aaatgtttcc agataaaaaa caaaaaggag gatagttata ccttttagga
    49381 tagcgccagg tctgtgtaaa cagatgacga ggagagggtt tatcgaaaga ttcggcggat
    49441 ggccctaggg actgcactct acagaaaatc cacaaaaaat gtctgcaaag ataaaacaaa
    49501 aggattttcc tgcagctgga agcattcata cgaattcatt atagaaagta ggatgttttt
    49561 tatgtgcgga attgttggaa tgattggatt aaaaaatgta acaccaggat tgattgacgg
    49621 tctagaaaaa ttagaatacc gtggttatga ttcagctggt atctttgtct cagatggcac
    49681 aactgattat ctggtcaaag cacaaggcag aattcaaaat ttgaaaaaca aaataaatat
    49741 tgatactaca ggaaatatag gtattggtca tacacgatgg gcaactcacg ggcaaccttc
    49801 tgaagaaaac gcccatccac atacttccca aagtggacgc ttcgtgctcg ttcacaacgg
    49861 cgtaatcgaa aactttgaag aacttaaaat tgcttattta gcaaatgacc actttattgg
    49921 tgagaccgat actgagatta tcgctcatct aattgaaact ttcgcgaagg atactacaac
    49981 gcaagaggcg tttttaaaag ctttgcgggt catcaaaggt tcttacgctt tcgctttgat
    50041 tgatcggaca gcacctgatg ttatttacgt cgctaaaaat aaaagtccac tattaatcgg
    50101 actcggcgat ggattcaatg tgattgccag cgatgcaatg gctatgcttg ctcatacgaa
    50161 ggaattcgtt gagattgaag atgaagagat ggttaccgtc acatcagaaa aagttattat
    50221 tcaaaatttt gcaggtgaca tcgtagaacg ttcttcattc gaagcacaag tagatgcttc
    50281 agatatcgag aaaggtacct atccttttta tatgctgaaa gaaatcgacg aacagcctat
    50341 tatcatgcgt cgtatcgctc aaaaatacgt aacagaagac aatcgtgtct ctcttgatca
    50401 aaagctggtc gatacattgt ctgatagcga tcgaatctat atcgttgctt gcggaactag
    50461 ttaccatgcg gggttagcag gtaaacagac tttggaaaaa ttgacacaga ttcctgttga
    50521 ggttcattta gccagtgaat ttggctataa cacacctctt ctttcaaaaa aaccattctt
    50581 tattttcttg agtcaaagtg gcgaaacagc agacagccgc caagtgctgg taaaaatcaa
    50641 ccgtctcggc tacccttcac ttacgattac aaatgttgct ggttctactc tttcgcggga
    50701 agcatccttt acactgctat tgcatgctgg acccgaaatc gcagtagcat caacgaaagc
    50761 ttatactgca caaatagctg tactaacttt attagccaaa gctatcggtg ataaaaaaga
    50821 atttgaaact tctcttgtat tcgatatcac tcatgaattg agtttagttg ctaatgcgat
    50881 ggaatcggtc atcgcccaaa aagattattt agaagagttg gcagaagtat atctcagcga
    50941 tacaagaaat gccttttaca ttggacgtgg tgctgactac aacgtttctc ttgaagctgc
    51001 tttgaaattg aaagaaatca gttatatcca ggcagaaggt tttgccgctg gcgaattgaa
    51061 acatggaacg attgctttga tcgaagaagg aacaccagtg atcggaatca tcagtgaaga
    51121 agtgactggt gcgcatactc gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac
    51181 actagttatt gtctcagagg gattagaaaa ggaaagtgac cagctggtgc taccaactgt
    51241 ccacccttat ctaactactc taaccacagt cgtaccgact caattacttg catattacgc
    51301 aacgctatta cggggattcg acgttgacaa accacgtaat ttagcgaaat cagtgacagt
    51361 agaataataa aagaacaagg accagtgtca gacatctgac actggtcctt gttcttttat
    51421 tttttatgaa aatatctctt aatccagtcc gatttcttct ttgactactg ccgcgatttt
    51481 atcgacatag taattaactt tctcatcagt aggcgcttct gccatcactc gaagtaatgg
    51541 ttctgtaccg gaaggacgaa ctaggattct tccttctgat cccatttcag cttctgcctc
    51601 ttcaatcact tttttgattg ccggaacatc catcgcaccg tttttatcac ttacgcggat
    51661 attcactaat ttttgcggat agattgttac ctctgctgca agctctgata atttcttacc
    51721 tgtctgtttc atgatattca gtaactgaat tcctgaaagc atcccatctc cagttgtgtt
    51781 aaaatctaag aaaatcatgt gcccagactg ctcgccgcca aagttataat cgtttttgcg
    51841 catttcttct acaacgtaac gatcaccaac ttgtgtaacg acatctttca aaccgattcc
    51901 ttccaccgct ttgtggaatc ctaagttact catgacagtc gtaacgatcg tatctttttt
    51961 caatcgattt ttttctgcca agtatttagc acagataaac atgattttat caccgtcgac
    52021 aatgtttcca agttcatcca ccgcaataat acgatcgcca tcgccatcga aagctagtcc
    52081 agcatctgct cctttttcaa cgaccatctc agctaaacgt tctggatggg tagagcctac
    52141 gccgtcattg atatttaacc cgtctggatt tgtgcccatt gtataaaaat ctgtttcaag
    52201 atcggcaaac aaacgattaa cacttgttga agttgctccg tttgctgcat cgacacaaat
    52261 ggtcaaaccg gaaaggtcac cgttgatcgt ttgtacaagg aattgagaat atttcaataa
    52321 gccttctgga aattcttcta cagttcccaa tccttcagct gatggacgag gcaattcatc
    52381 cacttcagca tctaataatg cttcaatttc tagctcttgg tcatctacta gtttaaaacc
    52441 atctgcaccg aaaaatttga ttccattatc ttgtgctgga ttatgagaag cagaaatcat
    52501 gacccctgcg cttgcttttt gcaaacgtgt cagataagca acacctggtg tagagatcac
    52561 acctagctgg aacacttcga tacccaccga taaaagtcct gaaatcagcg cttcttctaa
    52621 catttgacct gagattcttg tatcccgccc tactaacacg cgtgggcgtt ttccttcttc
    52681 ttcatgctgg cttaggacat aacctccgca ccgtcccagc ttaaatgcaa gttctggcgt
    52741 aagttccttg ttggcttcac cgcgtacgcc gtctgttcca aaatatttac ccatagttgt
    52801 aaaattactc ctttaactta attttctgag gaatttgttc tgctcatttg tgttgattcg
    52861 cttgtttctg atgtttcttc tgtacttgtg gtcgagctgc ttgtcccaga ttccgaactg
    52921 gaagaaatgg ttgttgtcga tggttccgtt cctgaatcag cagatgaaat acttctactc
    52981 gtacttgtat tttcttgtgt ccgactgctc gtcgaagcag agctactgct tgacgtttgc
    53041 aacacgggtg taatctccac actaactgat tcgggcttga tacttaaccc ctcttctact
    53101 ggtatttcaa ttttacgagt tgttcgatga ttgatacctg agacattgat cggtacagca
    53161 atactgtcac cgatcgatgc aagcaaatcg ctagagccgc ttacttctgc ttccagtttt
    53221 gaaaaactga aatcatagct ttctaccccg gcagctggtg ttccttgctg tatcccataa
    53281 agtcgtaccg acttgcttgg cttgatcgaa tcagctctca ctgttacttg cactggatca
    53341 gagacgatac ttaatacttc ccctgctgca ttcagcgcct gtattgggac ctcactatta
    53401 atcccatcat ctgtgaccat cgaagcgtcc acatcagcta ctactcgttt gatttctgcc
    53461 aatgttttgt cacctgtagt gatagagact tttttaggac taacggaagt atcatcaagt
    53521 tcatatccag atggtgtgaa cgttgaagga atcaacgttt ccacatcgaa atcttttatt
    53581 acctttttct caaccgttac tgtaatagtc gatggttcga tctttgctgt gacagcactg
    53641 ctcaaatttt gaatttttag cggaacttca tgtgttcctt cgcccagctt agtgatatct
    53701 gctgtgacgc ggaacatccg tgtatcttcg tttgcctctg cattcaactg aactcggttt
    53761 gcactggaaa gtttgacagt cactgcattt tcatatccat ggatatagta gcgctttgaa
    53821 tcataagaaa tcttgatcgg cacccccgta actgtttgat tatacgcttc atttccagaa
    53881 agcgtatttg gaacggactg gccatttgca ttgaagtaca gcgcaatact gaacagcaat
    53941 gcaataaaac tgtaaagaat gcttgtccgt ttctcttttg agatcatttg cgatcccctc
    54001 ccttagtcat accgtctagg aaagattgaa tcacgttttt cttgtttttt gttttctctt
    54061 cagcaattag ttctctacgc aagattgcca aatattcttg ctgggataag ccagccatca
    54121 gttcattatc taacgtgatg ctgacgtccc cggtttcttc agagacaacg atggtaatcg
    54181 cgtcactgac ttcgcttata ccgacagctg cccggtgtcg agtcccgaat tctttaggga
    54241 tcaacatact ttctgataga ggaagatagg cgcttgctac tgcgatcttt ccttctttta
    54301 caataactgc accgtcatgc agcggtgtat taggaataaa gatattgatc agcagctccc
    54361 cagtgatatc tgcatctaaa gcaatccctg tctcaatata ctcatctaat ccagtatgac
    54421 gttcgatagt gatcaacgca ccgatttttc gcttggacat atattggatg gctttgtcaa
    54481 aagacaagat catcttctca tcttcctgct gttcgctttt tgattgtttg aaaagcgaac
    54541 tgcgtccaag atgttccagt cctctccgga tttccggctg aaaaatcacg atagcagcta
    54601 tcaccccgta cgtaatgact tggttcatca gccaagacaa ggtgtgcaga ccaatgattt
    54661 ccgctacgat tcggataaca ataaaaatag ctacaccttt cagaagctga atcgcttttg
    54721 tgcctcgcag cagttggatc agtttgtata caagatacca gacaaccaga atatcaagta
    54781 tgttcaccaa aaaattcaga gagaacaagt caccggaaaa catttgctgc caatagtcca
    54841 aattaaaaag ttccccgatt tgaaatgaca taaaaagcct ctcttctctt ttgagatttc
    54901 ctctctagta tatcatatgt tttaatttta gaggctgata tgctcgtatt tttcttgaga
    54961 aaaacgcata attttcaaga aaattgcaag tatgcgtttt atttttctct gcaataaata
    55021 tttttaaact tcagagaatt attcattata ttttttctgt tatttttcct ttttttcttt
    55081 tgtagcttgt tccatttttt tacgtcgttc tgctctggca gctcttctag cagctcttct
    55141 ttcttctgct tcaaggtccg tcgttttttc ttttctagtc ttaacgggta catcgataga
    55201 ttcctctggt tcaagtcgtt tcttgatttt ttctaattct cccgtcagtg cagcatagtt
    55261 gtaaaaacgt gttttagcgt cgtggacagt cttttcaaat aaagcttttg caagatttgg
    55321 atttgcgtta tctagcgagg aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga
    55381 gaaatcaggt tttttgtagt ccaaagtcat tgggttcttg tttttttcga ccattgtagg
    55441 gttatatcga tataatgacc aataaccaga agaaacagct tcttttgctt ctttaagcga
    55501 ttctttcatt ccgccaagca agccatgtgt gatacaaggc gtataggcaa taatcagaga
    55561 aggaccagga tacttttctg cttcttcaaa tgccttgatc gtttgcattt gattggcacc
    55621 tgaagcaatt tgcgccacat aaacatttcc atatgtcatc gccatcattc ctagatcttt
    55681 ctttgaagca tactttccac tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt
    55741 tgaggtttga ccgccagtat tggaatagac ttcgttatcc aaaacaagga tattcacgtc
    55801 tgctccgctg gcaagtacat gatcgatccc gccaaaacca atgtcgtaag cccatccgtc
    55861 tccaccaatc atccattggc tgttttttac gaatagatcc tttctcttgt atatatcctc
    55921 cagttcaggg aaaattgatc gttcgctttc gatcgcagct tgcagttttg ctgctctttg
    55981 ctgcgttcct tcactctcaa acatgtgtac taaccagtct tccatcaatt ctcgtagttc
    56041 ttctgatact tttggcaata cttctttgat tgaacttgcc aaagcctcgc gacgagtctg
    56101 tgccgctaga agcataccat aaccaaattc agcattatct tccaataaag aatttgacca
    56161 tgcaggccct tgaccacatt cattggtcgt ataaggagaa gcaggagaag caccgcccca
    56221 aatagaagaa cagcctgtcg cattcgcgat catcattcga tcaccgaaca tttgtgtcaa
    56281 taattttaca taaggtgttt cgccacagcc tgagcaagcg cccgagaatt caagaagcgg
    56341 ttgattgaat tgtgtagaca agacagtatt ctttcctttg actggatttg ctttttgtct
    56401 taatgtcatt gcaaaagccc aattcacagc ttgttctttc tgttcttcat aaggcttcat
    56461 cactaaagct ttttcttttg cgggacaagc ctctacacac agaccacaac ctgtacagtc
    56521 ttccactgaa acttggatac ggtattttac gccgtctgcg cctcgcattt ctctcacgat
    56581 aaatccttct ggcgcttctt ccatttcctc ttcatctgct aaaaatggac ggatggcagc
    56641 atgaggacaa acaaaggcac attcattaca catcgtacag cgatcactaa tccactcagg
    56701 tacttctaaa gcgacgcccc gcttttccat agaagccgtt cctaaaggca tttcgccagt
    56761 tgtcattcca tttgctacaa gtgtaccaac agagagttga tttccttcct gtgcattcac
    56821 tggttcaagt atttcttgta tataaccagt gttcctttta ttgatttttt caggcatctc
    56881 caacgttctc caatggtctg ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat
    56941 tgcttgtatg tttttttcta cgatagccat tgatttatgt ccatagcttt tcagggcttc
    57001 ttcttttagc agcggaagca cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc
    57061 cgtttccatt gccgtattga tccggcgtcc caaaccgact tcttgggcca atctcatggc
    57121 attgatcgta tagaatctaa tgttattttc agccaagtaa cgttttaatt ttaagggcag
    57181 atgcgtttct aattgttcgt cgctccaaag agtattcaat aaaaaaatgc cacctggctt
    57241 caatcctttg accaagtcat agctatgcag gtaagctggt gtgtgacagg caacaaggtc
    57301 cgcatgctcg acaagatacg ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac
    57361 agtcagaccg ccagattttt tcgaatcata ataaaaatag ccttgcgcat atttatcggt
    57421 gtgatctcca ataatcttga tagctgattt atttgctcca actgttccgt cagatccaaa
    57481 tccccaaaac ttggcctgga atgtttgcgg ttctgtcaaa tccagacttt cctttggttc
    57541 caaagattga taagtcacat catccacgat ccctatggta aaccgttttt tgcgtatgct
    57601 tgggtctttt ttcaattcat caaaaactgc tgagatttgg tcgggagtca catctttaga
    57661 ccccaatccg tagcgaccac caataacagc cgggcgaagt tcagaatcat ataacgcact
    57721 ttgaacgtct aataagagag gttctccacc tgcccctggt tctttacttc gatcaagaac
    57781 agcaattgtt tttactgtat gaggcaactt ttgcacgaat gtttccactg gaaaaggacg
    57841 ataaagatgg atattcaaaa aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt
    57901 ttgttcaatc gtctgtgcaa ctgatcccat agaaacaatg acttcttccg catcgtctgc
    57961 tccgtaataa gtcaccaagt cataagaagt tcctcgtaat cggttgatct ctttcatata
    58021 tttttgaacg atttcaggta atttatcata ataggggttg atcgtttccc gttgttgaaa
    58081 atggatgtct ggattctggt tcgtaccact aacagtcgga tgattgggat tcatccctcg
    58141 tttacgaaat gaagctaatt tctcctgatt gatcagagga gctaattctt catattccaa
    58201 aacttcgatt ttttgaatct cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg
    58261 gacttctcct tcaatagaag caagatgggc aacaggtgct aaatccatca cttcttgtac
    58321 actgctttca gctaacatag caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc
    58381 tccgaaaata ttcaacgcat ttgtcgtcac tgcccgacta gcaacgtgga aaacacttgg
    58441 cagtagttcc ccagcaatct tgtacatatt cggtatcatc aaaagcagcc cttgtgaagc
    58501 ggtatatgta gttgtcagtg caccagtttt caatgatcca tggaccacac cagctgctcc
    58561 tgcttcggat tgcatttcta ctacttttac tggttgaccg aatacgtttt cttttcctgc
    58621 tgctgaccat tgatctacta attcagccat ggtcgaactg ggtgttattg gatatattgc
    58681 agcaatctcg gtaaatgcat aagaaatata tgcggctgcc gtattgccat ccattgtttt
    58741 catttttcgc atgattcatc atccttgttc atattctctc ttattatctg ttccattata
    58801 acgtaaaaca tgccattttt ttcttttatt tataaaaata ttttacatgt atctatttga
    58861 attggtcatc taatttttag acaaacagtc taaatcgttg cctttttttg tcagacatgt
    58921 ataaactata agcgaaagag gtgttttaac atggatatat cagtgattga tgcaacgaaa
    58981 acaaatacac aaaaaggaat cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa
    59041 ttcatcaact tagcttgtcc gtattgccga caatggtttg aagaatctta tgagctatta
    59101 gaagaagcag tccaaagcgg gcaattgcag cgagtgatca aattgttcga taaagaaaaa
    59161 gaaagcttac agcgagggaa cgtgatgcac cggtatctca caatcagcga tggccaaaaa
    59221 gcgatcaaag agatcaaaca aatattcgat actcaagacg agtggaaaca tctttctctc
    59281 caagaagtag cggattttgc agtagataaa ctaaagctga cagaacaaaa agacgaacag
    59341 ctgagccaag ctgtcatcaa cgaagctgag caagcacata ttcgatttgt tccaactgtc
    59401 atcttgggta aagaaatctt tgatgaatct atttcaatag aggaattaaa agaattgatc
    59461 caaacaaaat aaaaaaataa ggagcgtggg acaaaagtaa agaacacttt tgtctcacgc
    59521 tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac
    59581 gcttctgtcc cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga
    59641 ttatctcatt tcttatgaca agctaagcaa attatctcca ccaaataatt tattcatatt
    59701 tttgacttgc gcatactaac taattcttaa attttcatac aaaatttctc aatttccctt
    59761 aacgagtaat atttatgtac ggtgattctc actagtatcc caatcacttt ttaagttatc
    59821 ccgatttcaa ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg
    59881 gtcatttgtt gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc
    59941 tcgtctacca aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg
    60001 attcgtataa agctaacaac tattaactcc tctcagctaa tagttgctct cttttttatt
    60061 cagattcatt tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat
    60121 tgttccatta caacattcac tggctgatga aatggattag tagatttcga ttattcaaag
    60181 cgattttcta ttggtggatc gtgttcccaa ctaataagac ggattccaaa cctatcgtac
    60241 agatcttcta aaccacttat aaattttgca cctatttcag gtgttttatt gcgtggatat
    60301 tctattgtta cgccattaaa tggataccaa ttaggattag gctctctttt tttctgtaca
    60361 tctcccatag aatagttagc actttctggg acataatata ttcttcgacc atcctctaat
    60421 tcatctaata agatatttcg tgatcgtcta tcacttgcta aatgtagacg ccacgtgcga
    60481 taccatgcca tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct
    60541 tctatcacaa tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg
    60601 gtaatttctg gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt
    60661 cccagatgaa acacagctat aacaacaata atgattaata ttttgtttct atttgataaa
    60721 tttctcatta ttcgcacatc acctctacat tctcacatat tagttcgtaa tactccttta
    60781 atatcaaaat atctactggt cgtaataata ttcgctatga attcctacta ctcgtataag
    60841 aaaggccaaa tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat
    60901 tgttttctaa tcaccaaaat tgttaacaat cggttcagat atttcccagt tgattaaggt
    60961 aattccgtat tgtgtcaaaa cttcttgcgc ttctccgaaa ttaagtacag ttcttatgta
    61021 tccactttct cctaatataa agtctacctc tcttttaaaa ccttcctcga gatttctaag
    61081 ggctatatgt ggttttaatg acattgattg tacattttcc cccatataat attgcgcact
    61141 tcctcgacta aaatagattc ctagatcgtc cccaaaaggt agatcttctc taccactagc
    61201 taatctactt ctccatcttc gttctctttt catagaacct gcgctgaact cacacccgtt
    61261 aaactcagct atgacagtat cttcaattat gttaatctgt ccatctagtt cgtattcaat
    61321 cctaaatgga aattctccat aggtgatttc cggttcgggt attttaggtg agattaatgc
    61381 aaagaaagga aatattaagc ttggcaacat caaaaaaact cctcctatga tcacaattcc
    61441 cgatgtaatc caaaatataa tttgtacaat tctcattgat ccattgtatt ccattttatt
    61501 ccccctatta ctattcatat ccgcctatct atatcgatct tccaatgcag agataattgc
    61561 ttccatacct tgatttttta ctgagttacc tatgcgagat atcattctcc actaaggtgc
    61621 atcatattgt ctatctaaca tgattaattc tccaataggt gtatactcct ccaaaaacaa
    61681 catttaatgc atctccctcg acaacataag ctaccatgtt tgctgtatat gtgtttgagt
    61741 ctaattcata agcagataaa tttactgtcg ctggattaaa tataatcgca ttcgtattta
    61801 tagctactga atttgctgct tcagcaccac cttttgaatg tccgatcatt gtgatttcaa
    61861 tagtaggatt tacgtggaca gccctcaccg tttaccctga ccattataga aatcgcttac
    61921 ttttttgaac tacgcaacaa aggatacaat gctcctaact tcactgtctc catttttgtc
    61981 cactaaggaa tataaaaaat aggcattaat tcatttaatt cggtcacaac acgttacctt
    62041 ttaaatcatt atactgtatc tcatacatta ttatcaataa actattataa aaatgcaaaa
    62101 aatgctttat caatctattt tgtaattggt cataatatca ttgaaatcat accctgtatc
    62161 gatttttcct atgaatctcg tctatttgtg attgacgaaa aagaggcaag acagacactg
    62221 gaatggtaat tttacccact attttgctcc aactatttgt aatcgtaccc acttttgaaa
    62281 taccgagatg atcattttaa ttgatatgct gattcccaag caaaacccca agaaattaaa
    62341 tgctctatcg catctagctt cttgggtttt gcttatataa attccaacat ttaaattttt
    62401 tatgctgttc ggtctcagaa atgccgtttt tgcttatatc acaatatcaa gatgtcaaat
    62461 tattcgtgtt gccgatgact ttgattaatt tttttcaaat tgtttattaa gactaatgct
    62521 gtctttttgc agataccaat gttcgaactt tttaacatta tcatatgatc ctgcaagttt
    62581 taacttctta ccattagatg ttatcaaagt tataattaac cttgcctttt tcccattagg
    62641 atcttttatt ttatgaattt tctgcaggta atctctccta atggcagcta tttttacctc
    62701 atcagtgctg ataaaccatt gatcatccac atacatactg cctgttatgt tgtgtttttc
    62761 catttcttcc gagaacgaaa agtttaaggc tcgttcttac ttccaaagtt gaatgagtcc
    62821 atggattgga cttagtgtga gaattgagaa caacaataag aatccatatc ctcccgcaaa
    62881 catccctaag gctatactgt ctctccatcc ataagtagga ttagaagaga aaagagtcac
    62941 cgtttgtaat aatatgaaaa ggaataatcc agcacaaaca ccaaagataa caactccaaa
    63001 cattgaattt ttatacaatg accatttatt tataggcttt tctttcattt tacttccccc
    63061 tactgttctg ttcacacgga atgaattcaa agttcataag ccctcctctt tgaacatatg
    63121 aatacgactt gctaactata ctttaaaaaa gcttgtttta agcggttata ggcgagttgt
    63181 aatctctatt catctcatta gcaaataact tgccgaaaag tctactgaaa ttaaagcaga
    63241 tagtggaaga attatttagc caggatagac actatttgtg aaacacatgt ctttactttt
    63301 gaaattctat gatgaaaagc cttggaatac agtagctctt aattggtaat actttttcca
    63361 ttataaataa cctcttttca ttttatattt atcccctcta aaaataatat ctaatctaat
    63421 attttaaact tatatttccc tcgctgtgct aatagagcat tactaaataa atagaaacta
    63481 gaaaaaagca ttacgcctgc cagaataaac caattatttg tat
//
LOCUS       DAJSVD010000003        60722 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSVD010000003 DAJSVD010000000
VERSION     DAJSVD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.003"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            79..1011
                     /locus_tag="ON498_000112"
     CDS             79..1011
                     /locus_tag="ON498_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938417.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1232..2995
                     /locus_tag="ON498_000113"
     CDS             1232..2995
                     /locus_tag="ON498_000113"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3938418.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3079..3537
                     /locus_tag="ON498_000114"
     CDS             3079..3537
                     /locus_tag="ON498_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3938419.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3646..4329
                     /locus_tag="ON498_000115"
     CDS             3646..4329
                     /locus_tag="ON498_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3938420.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4476..5090
                     /locus_tag="ON498_000116"
     CDS             4476..5090
                     /locus_tag="ON498_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR3938421.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5106..5312
                     /locus_tag="ON498_000117"
     CDS             5106..5312
                     /locus_tag="ON498_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938422.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5853..6983
                     /locus_tag="ON498_000118"
     CDS             5853..6983
                     /locus_tag="ON498_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3938423.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            7075..7830
                     /locus_tag="ON498_000119"
     CDS             7075..7830
                     /locus_tag="ON498_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3938424.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            7888..9927
                     /locus_tag="ON498_000120"
     CDS             7888..9927
                     /locus_tag="ON498_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR3938425.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQIDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGILLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(10036..11187)
                     /locus_tag="ON498_000121"
     CDS             complement(10036..11187)
                     /locus_tag="ON498_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3938426.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11341..11871)
                     /locus_tag="ON498_000122"
     CDS             complement(11341..11871)
                     /locus_tag="ON498_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3938427.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(11970..12320)
                     /locus_tag="ON498_000123"
     CDS             complement(11970..12320)
                     /locus_tag="ON498_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR3938428.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(12477..14006)
                     /gene="ahpF"
                     /locus_tag="ON498_000124"
     CDS             complement(12477..14006)
                     /gene="ahpF"
                     /locus_tag="ON498_000124"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3938429.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(14016..14579)
                     /gene="ahpC"
                     /locus_tag="ON498_000125"
     CDS             complement(14016..14579)
                     /gene="ahpC"
                     /locus_tag="ON498_000125"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR3938430.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(14723..15385)
                     /locus_tag="ON498_000126"
     CDS             complement(14723..15385)
                     /locus_tag="ON498_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3938431.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            15569..17215
                     /locus_tag="ON498_000127"
     CDS             15569..17215
                     /locus_tag="ON498_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3938432.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            17206..18288
                     /gene="uxuA"
                     /locus_tag="ON498_000128"
     CDS             17206..18288
                     /gene="uxuA"
                     /locus_tag="ON498_000128"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3938433.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(18396..19949)
                     /locus_tag="ON498_000129"
                     /pseudo
     CDS             complement(18396..19949)
                     /locus_tag="ON498_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            20097..21238
                     /locus_tag="ON498_000130"
                     /pseudo
     CDS             20097..21238
                     /locus_tag="ON498_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(21364..22443)
                     /locus_tag="ON498_000131"
     CDS             complement(21364..22443)
                     /locus_tag="ON498_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3938434.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(22546..22986)
                     /locus_tag="ON498_000132"
     CDS             complement(22546..22986)
                     /locus_tag="ON498_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3938435.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(23043..23453)
                     /locus_tag="ON498_000133"
     CDS             complement(23043..23453)
                     /locus_tag="ON498_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3938436.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(23633..24067)
                     /locus_tag="ON498_000134"
     CDS             complement(23633..24067)
                     /locus_tag="ON498_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3938437.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(24167..25015)
                     /locus_tag="ON498_000135"
     CDS             complement(24167..25015)
                     /locus_tag="ON498_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3938438.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            25404..27170
                     /locus_tag="ON498_000136"
     CDS             25404..27170
                     /locus_tag="ON498_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR3938439.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            27272..28531
                     /locus_tag="ON498_000137"
     CDS             27272..28531
                     /locus_tag="ON498_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3938440.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            28678..29979
                     /locus_tag="ON498_000138"
     CDS             28678..29979
                     /locus_tag="ON498_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3938441.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            29982..30833
                     /locus_tag="ON498_000139"
     CDS             29982..30833
                     /locus_tag="ON498_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3938442.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            30846..31661
                     /locus_tag="ON498_000140"
     CDS             30846..31661
                     /locus_tag="ON498_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR3938443.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            31812..32978
                     /locus_tag="ON498_000141"
     CDS             31812..32978
                     /locus_tag="ON498_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3938444.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            33208..33855
                     /locus_tag="ON498_000142"
     CDS             33208..33855
                     /locus_tag="ON498_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3938445.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            33939..34883
                     /locus_tag="ON498_000143"
     CDS             33939..34883
                     /locus_tag="ON498_000143"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR3938446.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            35018..36469
                     /locus_tag="ON498_000144"
     CDS             35018..36469
                     /locus_tag="ON498_000144"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR3938447.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            36490..37326
                     /locus_tag="ON498_000145"
     CDS             36490..37326
                     /locus_tag="ON498_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3938448.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            37326..37985
                     /locus_tag="ON498_000146"
     CDS             37326..37985
                     /locus_tag="ON498_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3938449.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            38111..38800
                     /locus_tag="ON498_000147"
     CDS             38111..38800
                     /locus_tag="ON498_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938450.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            38806..39762
                     /locus_tag="ON498_000148"
     CDS             38806..39762
                     /locus_tag="ON498_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR3938451.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            39806..40318
                     /locus_tag="ON498_000149"
     CDS             39806..40318
                     /locus_tag="ON498_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938452.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(40574..41122)
                     /locus_tag="ON498_000150"
     CDS             complement(40574..41122)
                     /locus_tag="ON498_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3938453.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(41211..42392)
                     /locus_tag="ON498_000151"
     CDS             complement(41211..42392)
                     /locus_tag="ON498_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR3938454.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(42628..44136)
                     /locus_tag="ON498_000152"
     CDS             complement(42628..44136)
                     /locus_tag="ON498_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR3938455.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            44295..44486
                     /locus_tag="ON498_000153"
     CDS             44295..44486
                     /locus_tag="ON498_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938456.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            44689..45606
                     /locus_tag="ON498_000154"
     CDS             44689..45606
                     /locus_tag="ON498_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR3938457.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            45599..46672
                     /locus_tag="ON498_000155"
     CDS             45599..46672
                     /locus_tag="ON498_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938458.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            46820..47338
                     /locus_tag="ON498_000156"
     CDS             46820..47338
                     /locus_tag="ON498_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938459.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            47319..47711
                     /locus_tag="ON498_000157"
     CDS             47319..47711
                     /locus_tag="ON498_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR3938460.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            47787..48722
                     /locus_tag="ON498_000158"
     CDS             47787..48722
                     /locus_tag="ON498_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR3938461.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            48855..49634
                     /locus_tag="ON498_000159"
     CDS             48855..49634
                     /locus_tag="ON498_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3938462.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            49627..49944
                     /locus_tag="ON498_000160"
     CDS             49627..49944
                     /locus_tag="ON498_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3938463.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            50188..51798
                     /locus_tag="ON498_000161"
     CDS             50188..51798
                     /locus_tag="ON498_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3938464.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            51955..52803
                     /locus_tag="ON498_000162"
     CDS             51955..52803
                     /locus_tag="ON498_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3938465.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            53034..53513
                     /locus_tag="ON498_000163"
     CDS             53034..53513
                     /locus_tag="ON498_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938466.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            53546..55774
                     /locus_tag="ON498_000164"
     CDS             53546..55774
                     /locus_tag="ON498_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3938467.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            55903..57546
                     /locus_tag="ON498_000165"
     CDS             55903..57546
                     /locus_tag="ON498_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3938468.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(57619..58164)
                     /locus_tag="ON498_000166"
     CDS             complement(57619..58164)
                     /locus_tag="ON498_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR3938469.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(58187..58672)
                     /locus_tag="ON498_000167"
     CDS             complement(58187..58672)
                     /locus_tag="ON498_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3938470.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(58662..59474)
                     /locus_tag="ON498_000168"
     CDS             complement(58662..59474)
                     /locus_tag="ON498_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR3938471.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(59613..>60722)
                     /locus_tag="ON498_000169"
     CDS             complement(59613..>60722)
                     /locus_tag="ON498_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3938472.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        20258 a        10872 c        11813 g        17779 t
ORIGIN      
        1 ttgctagctt tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa tccgtatgta
       61 gaagaagctt gtaacgagtt gattgattat gtagagactc atttttttaa aagaagatat
      121 tcggaagatg aaaagtcctt tatcatacga gaaatagaga tcgcaatcgg aagaaaggca
      181 tttccccttg gactgaaaga agaagataag aaaagcgcag aaacgaagcc gttatttcaa
      241 ttagtctgtc aaggattgga agagcaaaaa ggaattctag acttacaaga agaagatgtc
      301 tattatattt tttctttgtt caacagtaga aattatacca atgagaacat ggagctgtta
      361 agaaaagatt tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa
      421 ttgattttac agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag
      481 caatcctttt tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa
      541 aggatctatc ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg
      601 gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa
      661 ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt
      721 tttgttgttt cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat
      781 gcggaagaag gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct
      841 gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat
      901 ggattagaaa ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat
      961 ttgtatcata ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt
     1021 aattaggaga ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct
     1081 gattcgtgcg aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat
     1141 tgacttagag tcaactctaa gtagtagaat agataaagtg taacaaatct attttcatta
     1201 aaagaagata gtacggagga gagaatgtat tatgagtaca gtattacatg tattggatgc
     1261 aatcaatagc cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac agttggcaga
     1321 agagatgcgc caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa
     1381 tctaggtgta gtagaattaa cgattgcgtt tcattatgta tttgattcac caaaggataa
     1441 aatcgtttgg gatgtttccc atcagtctta tggtcataag cttttaactg gtcgaaaaaa
     1501 tggttttgaa aaaggacatt accgtgaagt gagcggatat tccaaccaac atgaaagtaa
     1561 acatgactat tttacagtgg ggcacacatc tacatctatc agcttagcaa caggtttagc
     1621 taaagcgaga gatctgaaga aagaatcagg aaatgtggta gcatttatcg gtgacggctc
     1681 cctaagtggt ggcttagctt ttgaaggatt gaacaatggt ggtgttttac aatccaatct
     1741 aatcgtatta gtaaacgata atgagatgtc tattgatgag aatcatggca gtttatataa
     1801 aaacttagct gagcttcgtc aaaccaacgg taaaagcagc aataacctat ttaaagcaat
     1861 gggatttgat tatcgttacg tggataatgg aaacgatctg gaaacggtga tccgtttatt
     1921 tgaagaagta aaagatattg atcatccgat tgtacttcac gtccatacgg aaaaaggtcg
     1981 cggttatcag ccggcaatcg ataataaaat gaaatatcat tggcatgtac catttgatct
     2041 gtccaccggg gaggcaaagc aagtaacttc tggagaaaca tttaatatga tcatcatgga
     2101 ttatttggat aagaaaatgc aaacaagtac agctcctatt gccgcagtca atgcagcgat
     2161 accaggcgcc tttggactga aaacattcca agaaaaacat ccagatcatt attttgacgt
     2221 ggggatcgct gaacagcact ctatcacttt cgcttctgct ttggcttctc agggagtacg
     2281 tccagtcgtc ttccaatcaa gtactttctt gcaacgggca tatgatcaat tatcacatga
     2341 tttagcgatc aacgagaatc cagtgattat tttagtcggt ggcggaacga tctcagctag
     2401 tgaccgaaca caccaaggaa catttgacat tccttactta tcaagtatcc cgaacatcaa
     2461 atatcttgct ccagcaacaa aagaggatat gatctctatg ctagattggg cgttcgaaca
     2521 aacagcgcag cctgtagcaa tccgaattcc tacacatggt gtagaacatg gtacagctat
     2581 cgcaaatgat tatagtattc cagcctatca aacagtaaat aatggaggaa aagtagcaat
     2641 tttagcttta ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa
     2701 aggaatcgaa gcaacgttga tcaatccagg aacgatcaca gaaaatgacg tagaaacatt
     2761 agataaatta aaagaaacac acgaattagt catcacatta gaagatggta gtttgtcagg
     2821 aggatttggc gaaaatatca gtcgttatta tggtgattct tcaatgaacg tattaaattt
     2881 tggtgcagag aaagaattca cggatagcgt tcctgtaggg cagctttatg aacgttatca
     2941 tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc aataattcat
     3001 tatgctcacc atccagataa ttgtttatac tagtatctgg atggtttttt cattataaaa
     3061 agcaaagggg tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa
     3121 aaatatccta gttatcaatc agaaaaatta ggtttctttt catttgggga tacagaaaaa
     3181 atggcagacg agttgagcga actagttgta aaaaagataa agactggtac atcaagtggt
     3241 tacgatctgt ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc
     3301 ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata taaatgtgac catcttgcct
     3361 tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg gacattagct
     3421 tattggcgga aagtccatgc tgagtttttt aaacatgctt ataaagaagc gttgaacata
     3481 gaatttacag aagagaagct ggtcgtatac gaagaatttg aagtaatttt cttttagtaa
     3541 gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag ggttacattt
     3601 ttatatgaag ctgtattttt atataacatt actggaggag atattgtgga tacactggaa
     3661 caaatcaaag gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc gctacacagt
     3721 tcttttatta tggggagaat ggctttggct gcaaaagaag gcggagcagt tggtattcga
     3781 gcaaatacga cagaagatat caatgaaatc aagcgaatcg ttgatttacc ggtaataggg
     3841 attatcaaaa gagattacga tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg
     3901 gaagaacttt tggagacgca agcagaaatc attgcactgg atgcaacttc tcgtaaacgt
     3961 ccgacagaaa aaacaactgc tgaacttgtc caaatgattc acgaacacgg tcggttagct
     4021 atggcagata tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt
     4081 ttgtctacga ctttagcagg ctatacagaa tattcacgaa agacgcagac acctgatttt
     4141 gatttattga atcaaatcat agaacaagtc gatatcccag taattatgga aggacatact
     4201 gactctcctg aacaagtaga gaaagcatat gaattaggtg ctttttcagt cgtagttgga
     4261 tctattatca cccgtccgca attgatcact aaaagatatg tcgaagccgc aagtaaagca
     4321 aataactaaa aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt gatagatagt
     4381 ttaaagcaga ttctaagaag atgaaaaaag cattactttt cattttgtct tcaagatctg
     4441 ctttttaacg aattttatag aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat
     4501 tgacagaacg tgaaaattac aagttgctga ttggttcagt catccctaga ccagtagcag
     4561 tagtaacgac taaatcagca aaagatatag tgaatatcgc acccttcagt tacttcaata
     4621 tcgtcagttc gaatcgaccc atcttatcct tggcgatcca gagaaaacaa gatgcagtaa
     4681 aagacacagc aaagaatatt cttgagacaa gagaagctgt tatccatatc cttgatgagg
     4741 acaacgtaga agaagtaaat aaaacagcag ccaatctgcc taatgaggaa agcgaactgt
     4801 acctgactga cttgacttta actgattcag taatcatttc agtaccagca ttgacagaag
     4861 caaaagttcg ttttgaaaca tccttatatg aacatgtcga gatacggaat aaaaataaag
     4921 taacagctga tttatttttg ctagaagtac agaaatatca tttaaatgaa acagtctatg
     4981 atgataagac aggaaggatc gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa
     5041 atgattatgc agcaatagga gaaattttca cgattgcacg tcccgaataa aaaggagcac
     5101 tagttatgtt aaaaataagc tataaaccat cgacagattc aaaagaaatg aaaaaagaat
     5161 atgaaacagt gaatgatttt ctgcaagggc agtacttaga agttccgcca ttgcaagacc
     5221 attttgtcgt tactactgtt actttagatg gtaaggaaat agaaatgcca gatcaaacga
     5281 ttagcggatt gtttaattac tttaacaaat aaatatttat tccgaaaagt gaaaaaacat
     5341 ataaaaaaac acataaaact aaaaaattat caaagaatcc tggtgcaact gaaagaattt
     5401 gcaacgggat tctttcaact tatttgttat ttttttctaa aaaatattaa agattgttaa
     5461 atttcactat ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact
     5521 cgtaaataga atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt
     5581 gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt aaggcttttt
     5641 ccaatagcta agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga
     5701 tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact
     5761 gacttatttg atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg
     5821 agatgcggtg aaaaaagatc gattggtgga ttttgggtcc ttatttgact ttgtccatga
     5881 tcggtctttt agaagtatac agtgccagtt cttatcgttt attgcaagcc gatgaaaata
     5941 caaaaagtct attattgcgt caactaattt tcatattttt gagttggagc gtgatcttct
     6001 tagctcgttc agtcaaacta cactatttac ttcaccctaa aatagcagga tacggtttag
     6061 ccttatcgat tttcttttta gtattagtaa gaatagggat attcggtgtc actgtcaacg
     6121 gcgcacaacg ttggatctct ctgtttggca ttcaattcca gccttctgaa ctggcaaatc
     6181 tttttttgat tttttattta agctggtttt ttcgtgacgg aaatagtagc ccaaaagatc
     6241 taaaaaaacc attcctgatt acagtaggta taacttttct gattttattt cagccaaaga
     6301 ttgctggagc attgatgatc ctttcgattg cgtgggtcat attttgggca gcggcggttc
     6361 catttaaaaa agggatctat ctaatcgtta ctttttctgc attgctgatt ggagcagcag
     6421 gcggggtatt atatttagga aataaaggtt ggcttccaca gatgtttaat catgcttatg
     6481 aaaggatcgc gactttaaga gattctttta tcgatagtca tggtgcgggc tatcaaatga
     6541 cccattcgtt ttatgcacta tataatggtg gcatatttgg gcggggatta ggaaatagta
     6601 tcacaaaaaa agggtatctt ccagagacag aaacagattt tattttttcg attattacag
     6661 aagagcttgg gctaatcggt gcattgtgtg tgcttttctt attgttctca ttatgcatga
     6721 gaatcttttg tttaagcagt cgctgtaaaa atcagcaagc tggattgttt cttttaggat
     6781 tcggaacttt gctattcgtt cagacgataa tgaacgtggg aagtatcgcg gggctgatgc
     6841 caatgactgg tgtgccattg ccatttgtta gctacggagg aacaagttat ctgatccttt
     6901 ctttaggaat cggaatcacg ttgaatattt cttcaaagat acaggccgaa gagttggcgc
     6961 tctatcgacc tgaaaaacaa taaagagaaa ctgagaaact ctcagtttga attatcataa
     7021 agtacaatct tttatttttc aagcgagagg ttcttttaga aagaggaatt cgcaatgaaa
     7081 gtatctcaaa aaatcatatt atctattttg ttactgctta cattagtagt tggctatttt
     7141 tcgattgagt ttatccatgg attttcgtct gcaaaacaga gttcaactgt gaaaaaggta
     7201 gagccgaaaa gtatccctac cacactaaat gtggctttga ttggttcaga tgctcgttcg
     7261 aaagacgaaa acggtcgctc ggattcactt ctggttgcac aatacaacca gaaaacacaa
     7321 caagcaaaac tgatctctat catgagagat tcatatgtcg atattccagg ttacggaatg
     7381 aataagataa atgcagctta ctcttatggg ggagttgatt tattaaatca aacattaaag
     7441 gaaaatttca aatttgaagc tccctattat gcaagtatca catttcaaga ttttatcgat
     7501 tgcgttaatg aactgtttcc tggtggagtc aagatcgatg cagaaaaatc tctagattta
     7561 gatggcgtat atataaacaa aggagagcaa gtgatggatg gtaatacttt actccagtat
     7621 gctcgatttc gggaagacaa agaaggagac ttcgggagga tcagaaggca gcagcaagtg
     7681 atcaaagcta tctctcagca gctgaaagac gtaacttcaa tattcaagct acccaaagcg
     7741 gttgggaaat tacttggtag tatccaaacg aaccttccag aaagtgtgct tctggattgt
     7801 ggtatggatt tcctcaaaga catatgttag aataaacagg tataaatagt gaaataaagg
     7861 aatgacaagc aagagaagga ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga
     7921 acaggcgctt atattgccgg cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt
     7981 tactaccagc actatcaaga aacccaagca gtagaagctg gagaaaagac agttgagcaa
     8041 tttgtccaag ctttgaacaa gggagattat aacaaagctg cagaaatgac atcaaaaaag
     8101 gcggcaaata aaagtgcatt atctgaaaaa gagatcttag ataaatacca gaatatatac
     8161 ggtgctgctg atgtcaaagg acttcagata tcgaatctaa aagtagataa aaaagacgat
     8221 tctacttaca gtttttcata taaagcaaag atgaatacct cattaggtga attgaaagat
     8281 ctttcttata aaggaacatt agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc
     8341 aacttggttt ttccagaaat ggaaggaaat gacaaagtaa gcctgaccac acaagaagca
     8401 gcaagaggga atattataga tcgaaatggt gaaccattag caacaacagg caaactaaaa
     8461 caattaggag tcgttccaag taaacttgga gatggaggcg aaaaaacagc caatatcaaa
     8521 gcgattgctt cctccttcga cttaacagaa gatgctatca atcaggcgat ttcacaaagc
     8581 tgggtacaac ccgattactt tgtcccattg aaaatcattg atggagcaac gccagaactt
     8641 ccagctggag ctaccattca agaagtagac ggcagatatt atcctttggg tgaagcagct
     8701 gctcagctga ttggttacgt gggagatatc acagcagaag atattgataa gaatccagaa
     8761 ttaagcagta atggaaaaat cggtcgctca ggtttggaga tggcttttga taaggatctt
     8821 cgtgggacaa caggtgggaa attaagcatt acagatgcag acggtgtaga gaaaaaagtt
     8881 ctgatcgaac atgaagttca aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca
     8941 caaaaaacag cttttgacag tctaggagga aaagctggat caactgttgc gacaacgcca
     9001 aaaaccggtg atcttcttgc gcttgctagc tctccaagct atgatccaaa caaaatgaca
     9061 aacgggatct cacaagaaga ttacaaagct tatgaagaaa atcctgaaca tccattcatc
     9121 agccgatttg cgacaggtta tgctcctgga tctacgttta aaatgattac agcagcaatc
     9181 ggtctcgaca acggcactat cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg
     9241 caaaaagaca gttcttgggg atcttatcaa gtaacgcgtg tcagtagtga tgtatcacaa
     9301 atagacttaa aaactgcttt gatctattcc gataatatat atgcggcaca agaaacgttg
     9361 aaaatgggtg agaaaaaatt tcgtacaggc ttagataaat tcatttttgg tgaagacctt
     9421 gatttgccaa ttagtatgaa tccagcacaa atttctaatg aagatagctt taactcagat
     9481 atcttgctag ctgatactgg atatggacag ggcgaacttc taattactcc tatccagcaa
     9541 gcagcaatgt attctgtttt taccaacaat ggcacacttg tctatcctaa attgattgca
     9601 gataaagaga caaaagataa gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg
     9661 ccagatctga gagaagttgt gcaagatgta aatggtacag cacattctct ttctgcttta
     9721 gggattctgt tggcagcgaa aactggtaca gcggaaatca aagaaaaaca ggatgtaaag
     9781 gggcaagaga acagtttctt gtttgctttc aatcttgata accaaggtta tatgatggtc
     9841 agcatgttgg aaaataaaga agatgatgat tcagcaacca aacgagcatc ggaactattg
     9901 caatacctca accaaaatta tcaataaaaa attgcttttt aagtacagta aaaggactgt
     9961 ggcgttatcg tcacagtcct tttttcaaat aaacggtttt cacgatgtct ctttcacaac
    10021 ttctttttga tttactcaca cttcataaac tttttttgta aaatatttga gaacaagtgg
    10081 agaaataagt gtacttaata aaatcacgat aactaaaggt gaatagtact gtggttcgat
    10141 caattgactt tgctgtccaa tttgtaaaat gatcaaagcc atttctccac gcgaaatcat
    10201 accagcaccg accatcaatg aactatgttg agaaaaaccg gcaatctgag caccaaaata
    10261 acctccaagc aatttggtca aaatcgctac aagcgtcaat atagaaataa agagtagttg
    10321 ttcactaaag tcagaaaaat caacttctaa tcctacactg acaaaaaata ctggtataaa
    10381 tactgcatat cctaaagcct ctacattgtg gtatacttct tctttaacct ttgtctgact
    10441 gacagctatc ccagcaaaaa aagcaccaat gacagagcta agcccaacaa gatccgctac
    10501 gtaagacatt cctaaacaaa taactagcga tacgatgata atagaagaat ttgcgtacac
    10561 tttttctgca aatgaaagta ggaaaggcgc aatccatttg actaataaga aaataaaaac
    10621 aaaataaatt agctgttcaa tcaaaacaag cggtaaagaa gcattgctag aagcttcccc
    10681 cgttagaaaa gataggctga aacttagaac taaaacgact aaaatgtcat ctaccacaga
    10741 agcacccaaa attgttgatc cttcttttgt attcactacg tttagttctt ttaatacttc
    10801 tactgaaata ctgacagaag ttgccgcaag aataatccca aagaaaatcg cttcatttcc
    10861 cgaaacttgg aatgctttgc ctgtcaacca gccaaaaaca accgggaaga tgatcccaag
    10921 caaagcaacg aacataccag gtcggaaata ttttttcaaa agagataaat cactttccag
    10981 accagctaaa aacattaata agatcacacc aatctccgaa aagtcatgga ctagaatatc
    11041 aggtttgacc caattcaatc cagcttggcc tagcaaaaca ccaacaagta attgtccaat
    11101 cactgctggg atacctaatc tccgcgagat atgtgagcca atcgttgttg aaatcaatat
    11161 caggcataaa ataccaataa attccattct catcattcct ttgtataaaa tatagcccga
    11221 acttagcata acatacatta ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa
    11281 tccaaaagat ttttttggat tgttggtttt tatacggtct attttaataa ctaaaaatga
    11341 ttaacgtata gtcatttttc ttattctgat gaaattgata atctcctgga tgcccatgaa
    11401 aatcaaaata aagccgaata tttggaatag aagcatcaaa cttttgaatg gattgaacaa
    11461 taagacgaat ccgccaatca acaacagaat actactataa acactccaac ttttagcttt
    11521 tgtattcatg ctgaagaaca actggaacaa gctgttgatg ataacagcta atccaagaag
    11581 aatcggcagg atagaaacga ttgcaggtgc aaaagcatag acgatcagag ctaagatcag
    11641 gaaaaaaaga ccactgaaca gaccgatact ccaagaacct gttttccttt taaaacggta
    11701 agcctcaaaa agattcaaca acccaagcaa aacaagatag gcggtgatca gataaccgat
    11761 aaaatgaaaa acagcagaag ggtttagcac aatcgcaatc ccagaaataa tataaacagc
    11821 agctcttaat aaggcgtatt tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa
    11881 cctcctttga cttattttaa cgtgaactag tcattttacc aaattaaatg ttacaaaatg
    11941 agcgaaggag aagaatatct ctggaaaaat caacttctga ggatcttttc catttttttg
    12001 ccttttgcta gctcatcgat cattttatct aaataacgaa tttctttcat caagttatcc
    12061 tcgatgtctt ctactcgtac accacaaaca acacctgtaa tcaaggaacg tgaaggattg
    12121 gggacagggg cttccgcaaa aaattgttcg aaagtcactt gtttttgtag ttgatattct
    12181 agttctgttt gggaataacc cgtcagccaa caaatgattt catctacttc ctcttttata
    12241 cgatcttttc tctccgcttt tgctacatac ataggataaa cgcttgcaaa acttgtcgta
    12301 aaaatacgat tttgcgccat aatcagtcac ctttcttttt ttcaaattat ttactactta
    12361 tagtatataa gaaaaagaga agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac
    12421 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
    12481 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
    12541 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
    12601 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
    12661 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
    12721 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
    12781 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
    12841 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
    12901 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
    12961 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
    13021 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
    13081 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
    13141 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
    13201 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
    13261 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
    13321 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
    13381 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
    13441 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
    13501 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
    13561 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
    13621 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
    13681 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
    13741 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
    13801 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
    13861 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
    13921 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
    13981 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
    14041 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
    14101 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
    14161 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
    14221 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
    14281 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
    14341 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
    14401 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
    14461 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
    14521 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
    14581 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
    14641 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
    14701 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
    14761 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
    14821 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
    14881 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
    14941 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
    15001 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
    15061 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
    15121 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
    15181 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
    15241 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
    15301 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
    15361 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
    15421 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
    15481 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
    15541 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
    15601 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
    15661 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
    15721 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
    15781 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
    15841 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
    15901 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
    15961 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
    16021 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
    16081 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
    16141 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
    16201 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
    16261 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
    16321 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
    16381 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
    16441 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
    16501 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
    16561 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
    16621 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
    16681 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
    16741 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
    16801 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
    16861 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
    16921 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
    16981 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
    17041 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
    17101 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
    17161 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
    17221 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
    17281 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
    17341 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
    17401 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
    17461 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
    17521 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
    17581 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
    17641 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
    17701 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
    17761 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
    17821 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
    17881 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
    17941 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
    18001 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
    18061 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
    18121 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
    18181 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
    18241 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
    18301 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
    18361 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
    18421 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
    18481 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
    18541 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
    18601 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
    18661 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
    18721 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
    18781 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
    18841 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
    18901 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
    18961 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
    19021 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
    19081 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
    19141 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
    19201 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
    19261 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
    19321 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
    19381 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
    19441 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
    19501 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
    19561 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
    19621 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
    19681 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
    19741 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
    19801 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
    19861 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
    19921 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
    19981 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
    20041 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
    20101 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
    20161 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
    20221 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
    20281 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
    20341 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
    20401 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
    20461 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
    20521 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
    20581 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
    20641 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
    20701 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
    20761 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
    20821 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
    20881 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
    20941 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
    21001 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
    21061 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
    21121 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
    21181 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
    21241 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
    21301 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
    21361 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
    21421 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
    21481 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
    21541 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
    21601 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
    21661 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
    21721 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
    21781 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
    21841 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
    21901 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
    21961 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
    22021 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
    22081 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
    22141 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
    22201 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
    22261 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
    22321 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
    22381 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    22441 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    22501 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    22561 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    22621 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    22681 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    22741 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    22801 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    22861 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    22921 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    22981 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    23041 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    23101 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    23161 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    23221 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    23281 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    23341 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    23401 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    23461 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    23521 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    23581 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    23641 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    23701 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    23761 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    23821 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    23881 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    23941 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    24001 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    24061 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    24121 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    24181 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    24241 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    24301 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    24361 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    24421 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    24481 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    24541 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    24601 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    24661 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    24721 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    24781 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    24841 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    24901 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    24961 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    25021 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    25081 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    25141 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    25201 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    25261 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    25321 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    25381 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    25441 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    25501 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    25561 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    25621 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    25681 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    25741 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    25801 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    25861 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    25921 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    25981 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    26041 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    26101 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    26161 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    26221 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    26281 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    26341 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    26401 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    26461 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    26521 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    26581 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    26641 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    26701 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    26761 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    26821 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    26881 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    26941 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    27001 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    27061 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    27121 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    27181 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    27241 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    27301 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    27361 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    27421 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    27481 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    27541 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    27601 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    27661 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    27721 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    27781 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    27841 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    27901 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    27961 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    28021 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    28081 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    28141 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    28201 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    28261 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    28321 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    28381 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    28441 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    28501 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    28561 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    28621 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    28681 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    28741 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    28801 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    28861 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    28921 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    28981 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    29041 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    29101 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    29161 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    29221 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    29281 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    29341 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    29401 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    29461 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    29521 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    29581 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    29641 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    29701 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    29761 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    29821 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    29881 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    29941 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    30001 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    30061 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    30121 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    30181 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    30241 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    30301 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    30361 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    30421 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    30481 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    30541 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    30601 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    30661 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    30721 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    30781 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    30841 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    30901 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    30961 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    31021 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    31081 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    31141 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    31201 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    31261 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    31321 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    31381 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    31441 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    31501 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    31561 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    31621 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    31681 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    31741 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    31801 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    31861 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    31921 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    31981 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    32041 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    32101 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    32161 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    32221 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    32281 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    32341 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    32401 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    32461 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    32521 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    32581 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    32641 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    32701 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    32761 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    32821 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    32881 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    32941 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    33001 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    33061 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    33121 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    33181 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    33241 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    33301 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    33361 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    33421 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    33481 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    33541 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    33601 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    33661 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    33721 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    33781 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    33841 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    33901 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    33961 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    34021 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    34081 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    34141 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    34201 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    34261 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    34321 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    34381 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    34441 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    34501 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    34561 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    34621 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    34681 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    34741 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    34801 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    34861 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    34921 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    34981 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    35041 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    35101 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    35161 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    35221 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    35281 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    35341 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    35401 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    35461 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    35521 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    35581 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    35641 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    35701 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    35761 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    35821 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    35881 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    35941 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    36001 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    36061 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    36121 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    36181 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    36241 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    36301 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    36361 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    36421 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    36481 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    36541 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    36601 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    36661 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    36721 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    36781 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    36841 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    36901 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    36961 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    37021 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    37081 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    37141 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    37201 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    37261 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    37321 gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac
    37381 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg
    37441 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    37501 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    37561 ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    37621 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    37681 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    37741 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    37801 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    37861 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    37921 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa
    37981 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    38041 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    38101 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    38161 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    38221 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    38281 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    38341 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    38401 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    38461 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    38521 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    38581 aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    38641 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    38701 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    38761 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    38821 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    38881 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    38941 atcgaattat ttcaaaaaaa gcaccatcga atcgctgtga tccaaaatcc ccaagtagga
    39001 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    39061 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    39121 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    39181 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    39241 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    39301 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    39361 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    39421 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    39481 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    39541 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    39601 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    39661 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    39721 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    39781 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    39841 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    39901 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    39961 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt
    40021 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    40081 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    40141 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    40201 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    40261 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    40321 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    40381 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    40441 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    40501 ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta
    40561 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    40621 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    40681 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    40741 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    40801 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    40861 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    40921 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    40981 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    41041 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    41101 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    41161 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc
    41221 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    41281 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    41341 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    41401 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    41461 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    41521 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    41581 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    41641 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    41701 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    41761 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    41821 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    41881 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    41941 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    42001 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    42061 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    42121 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    42181 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    42241 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    42301 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    42361 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    42421 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    42481 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    42541 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    42601 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    42661 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    42721 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    42781 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    42841 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    42901 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    42961 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    43021 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    43081 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    43141 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    43201 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    43261 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    43321 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    43381 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    43441 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    43501 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    43561 atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac
    43621 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    43681 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    43741 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    43801 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    43861 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    43921 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    43981 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa
    44041 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    44101 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    44161 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    44221 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    44281 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    44341 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    44401 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    44461 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    44521 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    44581 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    44641 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    44701 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca
    44761 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    44821 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    44881 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    44941 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    45001 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    45061 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    45121 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    45181 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    45241 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    45301 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    45361 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    45421 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    45481 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    45541 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    45601 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    45661 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    45721 tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac
    45781 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    45841 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    45901 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    45961 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    46021 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    46081 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    46141 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    46201 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    46261 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    46321 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    46381 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    46441 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    46501 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    46561 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    46621 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    46681 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    46741 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    46801 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    46861 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    46921 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    46981 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    47041 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    47101 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    47161 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    47221 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    47281 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    47341 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    47401 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    47461 aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga
    47521 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    47581 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    47641 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    47701 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    47761 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    47821 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    47881 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    47941 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    48001 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    48061 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    48121 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    48181 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    48241 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    48301 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    48361 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    48421 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    48481 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    48541 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    48601 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    48661 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    48721 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    48781 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    48841 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    48901 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    48961 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    49021 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    49081 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    49141 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    49201 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    49261 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    49321 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    49381 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    49441 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    49501 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    49561 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    49621 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    49681 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    49741 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    49801 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    49861 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    49921 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    49981 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    50041 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    50101 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    50161 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    50221 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    50281 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    50341 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    50401 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    50461 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    50521 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    50581 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    50641 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    50701 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    50761 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    50821 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    50881 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    50941 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    51001 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    51061 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    51121 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    51181 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    51241 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    51301 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    51361 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    51421 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    51481 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    51541 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    51601 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    51661 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    51721 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    51781 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    51841 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    51901 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    51961 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    52021 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    52081 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    52141 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    52201 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    52261 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    52321 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    52381 gaaatacttg cacatatcga tcgaatccga gataatacct acgcttatct gattgtagat
    52441 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    52501 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    52561 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    52621 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    52681 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    52741 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    52801 taattccttt tattcaaaaa acataccaaa aatattccaa catcatcttg gactttctac
    52861 atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc aatgaagctc
    52921 tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt agtaaatgag
    52981 taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa
    53041 taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg
    53101 gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct cagtggaatg
    53161 atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac
    53221 gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta gctgcacaag
    53281 gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag
    53341 tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc
    53401 caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga
    53461 ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag
    53521 agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt
    53581 ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga
    53641 agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag
    53701 taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc
    53761 tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga
    53821 tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact
    53881 agaagctatt taccaaacag atacacttga agctgtactg acactgatcc agaaccatta
    53941 cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc
    54001 tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga
    54061 aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt
    54121 ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg
    54181 tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc
    54241 attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt
    54301 agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt
    54361 aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg
    54421 tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt
    54481 actccagtcg atttatccaa aagttttgta taaattatcc actgattcag atgaattggc
    54541 aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag
    54601 aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga
    54661 agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa
    54721 gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga
    54781 aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa
    54841 tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc
    54901 acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac
    54961 gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga
    55021 agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt
    55081 agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg
    55141 gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc
    55201 acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg
    55261 ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa
    55321 agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac
    55381 ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt
    55441 cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa
    55501 attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa
    55561 agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga
    55621 atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat
    55681 caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa
    55741 gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt
    55801 agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga
    55861 atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa
    55921 tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc
    55981 gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt
    56041 atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac
    56101 gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg
    56161 ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt
    56221 tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat
    56281 tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc
    56341 ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca
    56401 aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg
    56461 atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta
    56521 aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc
    56581 tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg
    56641 actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac
    56701 ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa
    56761 gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac ttcattggat
    56821 ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg
    56881 tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat
    56941 ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg
    57001 ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg
    57061 cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac
    57121 aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa
    57181 ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa
    57241 ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt
    57301 ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa
    57361 agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac
    57421 tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc
    57481 ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac
    57541 aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg
    57601 ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga
    57661 gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg
    57721 gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac
    57781 tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc
    57841 tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt
    57901 tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc
    57961 tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga
    58021 attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc
    58081 accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt
    58141 taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc
    58201 gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat
    58261 tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac
    58321 cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca
    58381 tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta
    58441 atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag
    58501 caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct
    58561 cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg
    58621 caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt
    58681 cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca
    58741 ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc
    58801 cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt
    58861 gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc
    58921 acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa
    58981 gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca
    59041 gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct
    59101 tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa
    59161 acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg
    59221 attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca
    59281 atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca
    59341 ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca
    59401 aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc
    59461 aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag
    59521 aaaaacagcc aattggcata tttttatctc atccaattta aaaaaacaaa aagaaaagaa
    59581 agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc
    59641 tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg
    59701 atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc
    59761 atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca
    59821 atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta
    59881 ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta
    59941 tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt
    60001 gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct
    60061 gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc
    60121 agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta
    60181 agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc
    60241 atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga
    60301 tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca
    60361 aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact
    60421 tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag
    60481 ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc
    60541 gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa
    60601 tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca
    60661 tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata
    60721 gg
//
LOCUS       DAJSVD010000004        54772 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSVD010000004 DAJSVD010000000
VERSION     DAJSVD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.004"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(109..1044)
                     /gene="murB"
                     /locus_tag="ON498_000170"
     CDS             complement(109..1044)
                     /gene="murB"
                     /locus_tag="ON498_000170"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3938473.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1117..2037)
                     /locus_tag="ON498_000171"
     CDS             complement(1117..2037)
                     /locus_tag="ON498_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3938474.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVFKDHYRHQL"
     gene            2150..2902
                     /gene="xth"
                     /locus_tag="ON498_000172"
     CDS             2150..2902
                     /gene="xth"
                     /locus_tag="ON498_000172"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3938475.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3766..4299
                     /locus_tag="ON498_000173"
     CDS             3766..4299
                     /locus_tag="ON498_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR3938476.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4789..5313)
                     /locus_tag="ON498_000174"
     CDS             complement(4789..5313)
                     /locus_tag="ON498_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938477.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5306..5779)
                     /gene="tsaE"
                     /locus_tag="ON498_000175"
     CDS             complement(5306..5779)
                     /gene="tsaE"
                     /locus_tag="ON498_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3938478.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5902..6882)
                     /gene="pta"
                     /locus_tag="ON498_000176"
     CDS             complement(5902..6882)
                     /gene="pta"
                     /locus_tag="ON498_000176"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3938479.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7035..7715)
                     /locus_tag="ON498_000177"
     CDS             complement(7035..7715)
                     /locus_tag="ON498_000177"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3938480.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7841..8710
                     /locus_tag="ON498_000178"
     CDS             7841..8710
                     /locus_tag="ON498_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3938481.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8736..9251
                     /locus_tag="ON498_000179"
     CDS             8736..9251
                     /locus_tag="ON498_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938482.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9298..9891)
                     /locus_tag="ON498_000180"
     CDS             complement(9298..9891)
                     /locus_tag="ON498_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938483.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9916..10095)
                     /locus_tag="ON498_000181"
     CDS             complement(9916..10095)
                     /locus_tag="ON498_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938484.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10172..10681)
                     /locus_tag="ON498_000182"
     CDS             complement(10172..10681)
                     /locus_tag="ON498_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938485.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10777..11157)
                     /locus_tag="ON498_000183"
     CDS             complement(10777..11157)
                     /locus_tag="ON498_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3938486.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11487..12236
                     /locus_tag="ON498_000184"
     CDS             11487..12236
                     /locus_tag="ON498_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR3938487.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12420..13697)
                     /locus_tag="ON498_000185"
     CDS             complement(12420..13697)
                     /locus_tag="ON498_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938488.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13776..14420)
                     /gene="pcp"
                     /locus_tag="ON498_000186"
     CDS             complement(13776..14420)
                     /gene="pcp"
                     /locus_tag="ON498_000186"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR3938489.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14619..15563)
                     /locus_tag="ON498_000187"
     CDS             complement(14619..15563)
                     /locus_tag="ON498_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR3938490.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15565..16251)
                     /locus_tag="ON498_000188"
     CDS             complement(15565..16251)
                     /locus_tag="ON498_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR3938491.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16725..17045
                     /locus_tag="ON498_000189"
     CDS             16725..17045
                     /locus_tag="ON498_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3938492.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17199..18104
                     /locus_tag="ON498_000190"
     CDS             17199..18104
                     /locus_tag="ON498_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3938493.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18101..18874
                     /locus_tag="ON498_000191"
     CDS             18101..18874
                     /locus_tag="ON498_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3938494.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(19055..20479)
                     /locus_tag="ON498_000192"
     CDS             complement(19055..20479)
                     /locus_tag="ON498_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3938495.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20690..21136
                     /gene="nrdI"
                     /locus_tag="ON498_000193"
     CDS             20690..21136
                     /gene="nrdI"
                     /locus_tag="ON498_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3938496.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21201..23183)
                     /locus_tag="ON498_000194"
     CDS             complement(21201..23183)
                     /locus_tag="ON498_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3938497.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23197..24093)
                     /gene="murQ"
                     /locus_tag="ON498_000195"
     CDS             complement(23197..24093)
                     /gene="murQ"
                     /locus_tag="ON498_000195"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3938498.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24096..25157)
                     /locus_tag="ON498_000196"
     CDS             complement(24096..25157)
                     /locus_tag="ON498_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3938499.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25368..25934)
                     /locus_tag="ON498_000197"
     CDS             complement(25368..25934)
                     /locus_tag="ON498_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3938500.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(25962..27464)
                     /gene="eat(A)"
                     /locus_tag="ON498_000198"
     CDS             complement(25962..27464)
                     /gene="eat(A)"
                     /locus_tag="ON498_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3938501.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27722..28843)
                     /locus_tag="ON498_000199"
     CDS             complement(27722..28843)
                     /locus_tag="ON498_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3938502.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            28969..29910
                     /locus_tag="ON498_000200"
     CDS             28969..29910
                     /locus_tag="ON498_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3938503.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(30060..30956)
                     /locus_tag="ON498_000201"
     CDS             complement(30060..30956)
                     /locus_tag="ON498_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3938504.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            31225..32565
                     /locus_tag="ON498_000202"
     CDS             31225..32565
                     /locus_tag="ON498_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3938505.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            32783..33226
                     /locus_tag="ON498_000203"
     CDS             32783..33226
                     /locus_tag="ON498_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3938506.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(33730..35148)
                     /gene="nhaC"
                     /locus_tag="ON498_000204"
     CDS             complement(33730..35148)
                     /gene="nhaC"
                     /locus_tag="ON498_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3938507.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(35551..36774)
                     /locus_tag="ON498_000205"
     CDS             complement(35551..36774)
                     /locus_tag="ON498_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR3938508.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(36805..37644)
                     /locus_tag="ON498_000206"
     CDS             complement(36805..37644)
                     /locus_tag="ON498_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3938509.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDIIENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(37661..38341)
                     /locus_tag="ON498_000207"
     CDS             complement(37661..38341)
                     /locus_tag="ON498_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938510.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(38343..39416)
                     /locus_tag="ON498_000208"
     CDS             complement(38343..39416)
                     /locus_tag="ON498_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3938511.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(39472..39710)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(39753..39986)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(40063..40734)
                     /locus_tag="ON498_000209"
     CDS             complement(40063..40734)
                     /locus_tag="ON498_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3938512.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(40821..43499)
                     /gene="mgtA"
                     /locus_tag="ON498_000210"
     CDS             complement(40821..43499)
                     /gene="mgtA"
                     /locus_tag="ON498_000210"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3938513.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            44158..44595
                     /gene="ndk"
                     /locus_tag="ON498_000211"
     CDS             44158..44595
                     /gene="ndk"
                     /locus_tag="ON498_000211"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3938514.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(44617..45483)
                     /locus_tag="ON498_000212"
     CDS             complement(44617..45483)
                     /locus_tag="ON498_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3938515.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(45643..47736)
                     /locus_tag="ON498_000213"
     CDS             complement(45643..47736)
                     /locus_tag="ON498_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3938516.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(48485..49108)
                     /locus_tag="ON498_000214"
     CDS             complement(48485..49108)
                     /locus_tag="ON498_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938517.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(49267..50664)
                     /locus_tag="ON498_000215"
     CDS             complement(49267..50664)
                     /locus_tag="ON498_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3938518.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(50696..51667)
                     /locus_tag="ON498_000216"
     CDS             complement(50696..51667)
                     /locus_tag="ON498_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3938519.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            52041..52466
                     /locus_tag="ON498_000217"
     CDS             52041..52466
                     /locus_tag="ON498_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938520.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(52954..53655)
                     /locus_tag="ON498_000218"
     CDS             complement(52954..53655)
                     /locus_tag="ON498_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3938521.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(53671..54111)
                     /locus_tag="ON498_000219"
     CDS             complement(53671..54111)
                     /locus_tag="ON498_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3938522.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(54111..54686)
                     /locus_tag="ON498_000220"
     CDS             complement(54111..54686)
                     /locus_tag="ON498_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR3938523.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        15822 a        11398 c         9573 g        17979 t
ORIGIN      
        1 ttattgtgtt aaaatgtaca agatttgcgc catttgcgta aacaaccaca aaaaagagca
       61 ggacggccac cgtcctgctc ttttacttat tttaagttcc attacttatt aattggctaa
      121 acttctgctt gtttctgata ttgcttgttt ttctccaatg atccgcactt ctgtttctaa
      181 agacacatca aatttttctt tgattacttc ttggatgtgc gcaatcaatt caatataatc
      241 tgtcgctgtc gcatgatcga tattgacgat gaatccagca tgtttttcag agacttgtgc
      301 accgccccat ttcaggcctt gcaatcctgc ttgttggatc agctgacctg tatagtgtcc
      361 tactgggcgc ttaaagacac ttccgcatga agggtattct aatggctgtt ttgattgacg
      421 caattcggtc aactcatcca ttcttgcttt gattgcttca taatcccctg tttgcaaact
      481 gaatcgagct tccaatacga tagcacgtaa ttcttggact ttactgtgac gataagaaaa
      541 taccatttcg tcttttgtca aagttttcaa tgttccatct tcaagcaaaa gatccacttc
      601 agcaaaaaca tctttgatct ctccgccata cgcacctgca ttcatataaa ccgctccacc
      661 gacacttccc gggatgccgc aagcaaattc aaatccagtc aatgattctg ccaaagctgc
      721 ataagtcgta tcaatcagtg aggcacctgc ttccgcaatg accatcgttc ctttgacatg
      781 aatctgtttc atttcagtca gcatgatgac tacaccgcga atcccgccat cttgaacgat
      841 cagattgctt gcatttccta ataccatcca aggtatatca tggattcggc aataatcgat
      901 gatttgcttt acttcttctt gttttttcgg gaatgcaagg acatcagctg gtccacctgt
      961 tttcgtaaac gtatagttca tcaatggttc atcaaacaat atagtaatcg tggctaagct
     1021 ttcaactatt tcttctttat tcactgagga ttttcctttc ttttctcaaa cgtcctcatt
     1081 attctatcac gcttaataca atcttgccag tcttgttcaa agttgatgac ggtagtgatc
     1141 tttaaacact tcctcacata attcatgact tcttacaact tctttttgtt taagcaattc
     1201 tgtttccgat ttacctaaac ggatctgaga aaagaaggcc tctaccattg gctcaaaccc
     1261 gcgtttgact agcgtgtttg tccagtcgcc aaattctttc actttttttc catcagcatc
     1321 ttgaatgaca agctgggtca gatcgtttac ttcataagta ccatttttac ttgttacttg
     1381 atatttttct atattagcgc cacttgctaa atccatcgtt aaaatagccg tttgattttg
     1441 tgtttctaat tgtaaaatgg catattccaa aaattccttt gtttctctta tctggtattt
     1501 tgttctcaaa atgggttcat ctaataaata cacagctgta tcaataagat gtaaaaacaa
     1561 atcataaagg acgaactctg tcgtttcttt tgccgctatt cgattttttt gcaggtggat
     1621 caaccgctta tctgggatac gtttcaattc ttctaccata ggagcaaagc gccggttgaa
     1681 accgatcatc aaaattattt ggttcttatc agctaattct tgcaattcac gaacttcatt
     1741 tagatggaca ctcagtggtt tgtcaatata aacatggata ccattttcta gacatttttt
     1801 ggctaattca taatgtactt ttgttgctgc gtggatcata catgcttcga ttttttcttc
     1861 taaaagctca tcgatcgttt catatagatg ttggaacccg tactcttttt gaattctttc
     1921 ttttgtttct ttatttcttg ttgcaaaata aaaatcaagt gttccttgtt ttttcgcata
     1981 agttggtaaa tatgcttttt ctgcgatatt ccctacaccg attacaccaa tcttcatcta
     2041 ctttcctcct ggatcattta gttactttca tcttacacca ttttttacta aaatgaggta
     2101 caatagtatc gcaaaggtaa ggaaattaat tcgaaggagt gtgcttttat tgaaatttat
     2161 ctcttggaac gtaaacgggc ttcgtgccat cgtaaataaa aattttttag aagtttttca
     2221 tgagttagac gccgattttt tctgtctgca ggaaacgaaa ctccaagcag gacaaattga
     2281 tttagatctt ccaggttatt atcaatattg gaattacgcg gaacgaaaag gctattccgg
     2341 cacagcgatt tttgctaaaa aaccagcatt gaatgccact tacggaatgg gtatcgatat
     2401 ccatgataca gaaggccgtc tgattacttt ggaatattcc gatttctttc ttgtaacctg
     2461 ttacacgcca aattctcaaa gcgaattaaa acgtttagat taccgattag aatgggaaga
     2521 agccttttat aattatttgg aaaatctaaa aaaacaaaaa ccagtcatcg tgtgtgggga
     2581 tctaaatgtc gctcatcaaa agattgattt gaaaaactgg aaaacgaatc aaaaaaatgc
     2641 tggattcact ccagaagaaa gagcggctct ttctcgactg ttggataacg ggtttatcga
     2701 tacattccgt tatttttatc caacccagga aggtgtctac tcttggtgga actaccgatt
     2761 caattcaagg aaaaacaatg ctggttggcg gatcgattac tttttgacga gcaaagattt
     2821 agagccagga ctagctgatg caaagatcca tacggacatt atgggcagtg atcattgtcc
     2881 agtagaatta gatttgaaat aaagaggttg tgaaaaagtc gtttagtttc gagtattaag
     2941 aaaaattttg tgaaaatggc tttccacatt ttgcataaat ttggcataat accgagaaac
     3001 tggcttttga acactgttta tcgagagatt gtgaaaaagc tgtcctgcat caagtaataa
     3061 gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttgggctta ttaccgcaga
     3121 tacaagcttt tgaacaccgt ttattaagag gttgtgaaaa agctgcactg catcaagtaa
     3181 taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc ttattaccgc
     3241 agatgcaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca ctgcatcaag
     3301 taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg gacttattac
     3361 cgcagatgca agcttttgaa caccgtttat tgagaagttg tgaaatcagc gtcctgacct
     3421 gagtattaga gaaacaaatg gcaaaatagt tcctcatatt ttgctatttt ttgagctaat
     3481 accgagggtc agctgattga acaccgttta gtggagaagt tgtgaaaaag tcgcctactt
     3541 tcgagtcgta agaaattttt ttgaaaatgg ttcctcacaa ctttgcataa atttgactta
     3601 ataccgagaa gtcatgatcc ccatctaact ccaaatcaca aggaatgagt tcaaaaacta
     3661 gcttttctca acaaatatta cataaaaaat tctatgtttt ttgttaaatt tatttgcttt
     3721 tttcaaaagt atgttacagt aatttcccaa ggaggtttta cagaaatgaa ttttgttgcc
     3781 atggattttg aaactgccaa tcatcagcct tatagtgctt gttctcttgc gctagtcatg
     3841 gtaaaaaata gccagatcgt tgatgaattc tatacattga tacagcctga gactcctttt
     3901 ttctggcgaa atgtccaaat tcacggaatc catcaagaag acgtacgcaa tgcgccgaaa
     3961 ttcccagaag tctggcaaac tatccaacca tattttcaaa aaaatcgttt agtcgttgca
     4021 cataatgctg cttttgatac aaaggtctta gcgggatgct tagattatta tcatcttgaa
     4081 cagcctaatt atctttcttt atgtacagtg aaaagtagcc gtcgcctgtt tcctgaattt
     4141 ccaaatcatc gattgaacac tgtgtgcgaa aatttgaaca tccaattaga tcatcaccat
     4201 gatgcattgg aagatagtcg tgcatgtgcg gaaatcctgc tttatcaaga aaaaaaattc
     4261 gggacagatc cattgaaaaa acttgtgact ataaagtaaa agaatactta tgaaacacac
     4321 aaagaggtcg tgagcctgcc gtttcactcc gagtcacaag gagcaatttc aaaaaatagc
     4381 ttctcatgtt tattaaatat tgtgactggt gaccgaggag ctggcaggag aacaccgttt
     4441 attggagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc aaacaaaaat
     4501 agcttctcat atttttcatt tggttggact tattaccgca gatgcaagct tttggacacc
     4561 gtttaccaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggcaa
     4621 aatagttcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
     4681 cgtttattca aaaaaaatgg actgcgacac gacttttgtc acagtccctt cgtgtgtttt
     4741 tctgccttct caaaaggatg acagtttctc actcttttcc tatttcttct attcaatcaa
     4801 ataactcatt ttatatacat ctaaaaattc acctagatct gttcttgccc ctctaggcat
     4861 gaccgcttcg atttggaagc ccatttttcg gtaaaggtgt acggctcttt cattacgaac
     4921 ttgaacgtca agctccaatc gaaaaagtac acccatttcc ttcgcccaac tgatgatttc
     4981 ttctagcata agagtgccta gtcccattcc ccaatattcc tgcagaatac tgataccgac
     5041 ttctcccacg tgagacaaac gaaattgact gtctgccttg acagatgctg ttccaataat
     5101 cgtcccttca tagattgcaa gcagcaataa attattattg ctttcgtata tataatccaa
     5161 ctcttcttct aaggtttctg gtggtaacag gagttctgct tcatccaaaa ctaaaaattc
     5221 tgtctcctta ttaacttgtc ccatcatcaa aagaatgtct tttgcatctc ctggcactgc
     5281 ttcccgcaag atgatcccaa cttcttcact catgattttc agcccatttt tctagaattc
     5341 gctgtttgaa ggtttcactt tgctccccgt atgagattag tttcacatac cgcttttcta
     5401 aatctgtatc acttttttct aaaatcagtt ccaaataatc ctccggcagt gcttcttcta
     5461 atagatttcc ccattcaaca acggaaagcc catcaccttc aaaatattca tctaacccta
     5521 aatctgctcc gctagctgct acccgataaa tatccatatg atacaacgga atccgacctt
     5581 gactatattc tctaataatc gtgtatgtcg gacttttgat catttgcgtt atccctaatc
     5641 cttgggcaat tcctttcgtc aacgttgttt tacccgcacc tagatcacca gtcaatatca
     5701 gattgtcacc tggcattgcg gaatgaccaa ttgcctctgc gaattgttct gtcattgtta
     5761 atcctgatag ttcgatcatt tttcatcttc tcccgctctt ctttcttttt gccagtatat
     5821 cgcgtaatga agttttttta aagaaaaaat gaactagtca agaacagcag ctgctgtttt
     5881 tggctagttc gcgtgtattt gttaatcaat caatgtttgt gctgctgtga taatcgataa
     5941 tttatataca tcttcttcat ttgctccacg agacaaatca gaaaccggtt tgttcaagcc
     6001 ttgtagaatt ggtccgattg cttcaaagtt accgaaacgc tgagcaattt tatagccgat
     6061 atttcctgac tgcaattctg gaaatacaaa gactgttgct tgaccagcta cttctgaatc
     6121 tggcgctttc agttgtgcaa ctgaaggaac ataagaagca tcaaattgca attcgccgtc
     6181 gatcatgtat tgtggcgcta gttttttagc gattttagtt gcttcaacta ctttgtctac
     6241 ttctggcgat ttcgctgagc cttttgtcga gaagctcatc attgctactt ttggttcgat
     6301 atcgaacaat tctgctgttt ttgcagaatc tacggcgatt tcagccaatt cttgcgcagt
     6361 tggattcacg ttgatcgcac aatccgaaaa gacatatttt tcttgatctc ttccacgtac
     6421 catgatcatc gctccgcttg tccgactgat gccaggcttt gttttgatga tctgaagtgc
     6481 tgggcgaaca gtgtcgccag tcgaatggat cgctccagag accattccgt ctgcaatccc
     6541 catatacgta agcatcgtac caaagtaatt cacatctttt aaaatctttt ctgcttgttc
     6601 tttggatgcc tttccatttc tacgttcaac aaaagtatcc accatttcat cccacttatc
     6661 gtaattatca gggctgatga tcgtaaagct agatgttttg attcctctgg catgcgctgc
     6721 atttcttatt tcttcttcgt tccctactaa aatgggttcc acaagttctt ctgctttcaa
     6781 ccgggcagct gcgcctaaaa tacgagcatc agtagcttcc gggaaaacga ttttgatccc
     6841 acggcgaaca attttaaatc ttaaactatc aaacaattcc acaagatgac ctccagacgt
     6901 ttttatatgt attatcatac actattccag aaaaaaagaa cagtttattt tatctgtatc
     6961 agtcaaaaat gctggaaaaa ttaagttttt cacatacaaa gcgaactgca ctataacagg
     7021 aaagcgctgt tttattacac tgtttcaggc agttgccaat caatcggttc ttctcctaac
     7081 gcaaggagcg catcattcgt tttcgagaag ggtttggaac caaaaaagcc acgacttgca
     7141 gataacggac tgggatgtgg agatgttatg atgatatggc gactcttatc gatcattttg
     7201 attttttctt gtgccggttt tccccacaag ataaacacga ttggtttttc ccgttcattt
     7261 aatttttcaa tgatcgtgtc tgttaatctt tcccaacctt tcccgcgatg agaatacgct
     7321 tgcccttccc gaacggtaag aacggtgttg agtaaaagga ctccctgttt agcccatgac
     7381 actagatttc catgttgaac aggtgcgatt ccaagatcat cataaagctc tttatagata
     7441 tttcttaacg atggcgggat ctttacgcca ggttgcacag aaaaagataa gccatgagct
     7501 tgattttctc catgataagg atcttgtcct aaaatcacca ctttaacttc ttcataagga
     7561 gtcaattcca atgcttcata gatatgatac atatctggat ggatcttttg ggacgcatat
     7621 tcttttttta aaaattctct tagatgcaga taataatctt ttgaaaattc tgaggacaaa
     7681 acttcttgcc aactgttatg aatgatttct ttcaaaaact ccacctccta ataaaaagaa
     7741 taactcacat tttcttcaat cacaagggac aatcttcacc ctcagcactt gattaatatg
     7801 gtaaactagg aacaagaagc agaaaggaac gataatcatt atgatcaaat taattgcatc
     7861 agatatggac ggcacattat tagatgccca tatgagtgtt tctcctgaaa atgccgaggc
     7921 gatacgtttt gcaaatgatg ccggtgttga gttcatggtc gctactggtc gaaactatca
     7981 agaagctcgc gcagctttag atgaggtagg aatcgattgt gcgatgatca ctttgaacgg
     8041 ggcacaggta tttgataaag aaggaaattc attgtttacc gtgtcgattc caaataatca
     8101 agcaatctct gttttagata ttctagatgc taacggtatt tactatgaag tagcaaccaa
     8161 cgatggtctt tattctgaaa atcaagcgaa acgaattgaa agttttgctt ccatggtggc
     8221 tacccattta cctcacctta cgtataaaat ggcgattgcc atggcctcag ctaagctgga
     8281 gttattgcac atcacatacg tggacagtat tcgtgctatt ttggaagaaa agaatctgga
     8341 agttttaaaa attatttgtt ttcatacaga agggccagct gtattaggtc cagttggaaa
     8401 gcagatttct cttttaggcg atctagccgt tacttcctct ggtcaaaaca atatcgaagt
     8461 gaaccataaa aacgctcaaa aaggaattgc tgttgcccat gtcgctcacg agcgtgggat
     8521 ttctttagaa gaagtcatga cgattggcga taacttcaat gatgtcagca tgctgcaagt
     8581 agcaggagtc agtttcgcaa tgggaaatgc agaaatcgaa gtaaaagatt atgccaaata
     8641 tacaacggac acaaaccttg aatctggcgt aggaaaagcc atattgcgcg cgattgcaga
     8701 agacttgtag ttttatctag aaaagaggga tatagatgtc agaatttttc attcaggaaa
     8761 agcagcttgg acgtgcaact cggacaattg tcaaaaacga aaaaggacat tccctgtttt
     8821 tgatggttgg tcgctgggga acaaagggcg atgcattgtc tctttatgcc atgaatggcg
     8881 atctcgttgc tcacatcaag cagataagcc tgacattcgg tacgcgtttt gagctgtata
     8941 aagagttcga aaaggtggga accatgcaga aaatattcaa ttggcccggt gacttttatt
     9001 atattcgtag acttcactgg acagtccaag gtgatatcta taatcatcaa tatcaaatcc
     9061 atcatttcaa tcagcaaatc atgaaaatgg ataaagcaac acttttttca ggagattact
     9121 atgtattgga tgtctcaaat gatgaagatg ctccagtttg tatttgtatt gctgcggttt
     9181 tagattactg gctctaccgt aaagacaaaa gtcgcgataa gcctgtttct taccgactta
     9241 acttaagtta aaaagatgta cctgaaatgc ggaaatacgc atttcaggta catcttttta
     9301 gatattcttt tgcatatgat cataaagatg accactgatc atccgctgac cgacaacttt
     9361 gaagtcatgg cgttcgtata attttttagc ctttggattt ttttcatcca cacttagacc
     9421 gatgacggag cgattagctt tcttcgccaa tttaggtaat gcttctaata atcgcgaacc
     9481 gatgccttgt cctctaaaat cttctgatac actgatagag tccaaatacc attcgttggg
     9541 aaacgtttct ttatcaatga acaaacgtgt agacgcatct agctgatatt tctctaagat
     9601 aggtaccagt ggctgatcga tgattgattc ttcttcatct gtataaccgt aagcaactcc
     9661 tgcaacttta ccgtctattt cttctacgat acctcgtgaa taactgtaac gataatttgg
     9721 atcagtgatt gcttcttcta aaatctctaa agtcttttgt tcccctgtct gcttcaaaaa
     9781 tggcagttcc atatctttta gaatcactaa gatcaatggg gcgatttctg gagcatcttc
     9841 ttttctcgca aaacgaatca tagtttcctc tccttttctc cattctaaca tgcagaaaaa
     9901 gaaatcgcaa aatcgttatt ttaaaaaatc ttcaatcttt tcagtaatga aataaccatg
     9961 agtaacgatt cttgccgctt cagttcggca ataagggcag acgactggca tttctttatc
    10021 attatgttcg actacattat tgacttcttc gtatgcaaat ttttttccgc aattagggca
    10081 tttcattttt tccatgttat ctcaacttcc tttcatcttt actctcttag cttactctta
    10141 tattgtaaaa aagagaagca atatccttct tctatgcaga tatccatacc tgacggtcct
    10201 gttctccatc tattaatcgg actttataat aaggacgttc atctttgact agaagtgtcc
    10261 actctttcac ttttgctttt ttcccgtagt agctgactgc ttccttcatc gctgcttgcg
    10321 gttgtttgaa ctcttccgtt ttttctatat caaaaagatg ttctgcggcc ttttccgtgt
    10381 catttcgtac ggttgttttc ccagtggatg gattgactgc tatgacttgt ccttcacaaa
    10441 aaaactggta ttcatatgtt gcattcgtag gattctccaa atctttttca actgcaaaat
    10501 gaatagcaga tatgttatgt tcttttgttt cgttttttac gattgctgct gcttttttca
    10561 atgagatccg atattcctgg tcactaatat catccatgtg aaaagcggca ttgagcagtc
    10621 ccgtcatacc ggtaatccct attacgatga gggcagcaac accaaacatc atttttttca
    10681 ttcttcattc tcctgtttct tttttttaaa gaatatcaag aaatgaacga atgaaaaatc
    10741 gaatgaaacg aaaaaaaagt gacaattttg ttatctttat tcgtgcagtt ctaatggcaa
    10801 tccgtctgga tcaaagaaaa acgtcatttt ttttcctgta aaggtatctt ctctaatcgg
    10861 ttctgattcg atccctaagg actgcaattc tgcaattgtt tcttcaatat cgtctacttt
    10921 gaaagcaaga tgacgcaagc cacaagcttc tggatagttt ggtcgttgtg gcgcattagc
    10981 aattccaaaa atctccaatt cagcacttcc tagtttcaga tctaatttat agtcatctcg
    11041 ttctttgcgg taattttctc tgattacttc aaatcctaat ttattaacat aaaaatcacg
    11101 tgatttctga taatctgaaa caatgatggc tatatgatgg atcgttgtaa ggttcatatg
    11161 agcactccta ttcttcgttt aagctcggat ctatattaat ctccagttcc attgtaaatg
    11221 tgtcagattg aaaaaaatag ctgtttttcc tttttataag aaaaaaggca aaaaaaacag
    11281 aaagtaggtc ctccaagaca ctcttcctgt tcccaatata ataaatgaaa gtaagaaatt
    11341 atattttcaa gaaaacaaac agaaaattat cataacacaa attatttata attgaaagac
    11401 tagttttttt tcaagaaaag catacaatag attgtgttag taaatacaga acacactaca
    11461 tttagtgtct taaggagtcc tcatacatga agtcatattt tcagttttta gctcatcagc
    11521 taaaaggctg gccgcaacaa aattattatc ttttcttttt cagtcttggc tgccaggtca
    11581 tgactcttgt caatagtccc atcacttggc tgtccgttat tacatttatc ggtaccacgc
    11641 ttggtgtatt gtgtatttta tccatcaatg cggcaaaatc agtcaacggt attttaggta
    11701 ttttgtctgc gatttgtttt attatcgttg ggttttcagc aaagaactat ttaagtatcg
    11761 gtgaacagat tgcttatatg atcacattgg atatccccgt attgttaagt aaagaatgga
    11821 atcataatat ggcttcaaaa atccgaaaat tcactggaaa aagctggttt atcgcaatca
    11881 tcagtacttt ccttgtctat cttgtttcag gattcctgat tggtcgttat acagatgatc
    11941 ctcggccgtg ggtagatgcc attgcttttt ctattagctt gactgctggt atcatcagtt
    12001 tcttgcgtta caacaaccaa tattactggt ggttagcttc agggcttgct caaatggtcc
    12061 tttggtatat ttcatttacg catggatctg caagtttggc gatgttcgtg aacagctctg
    12121 tttacttgct gaatgatgtc cttgcattta ccgtttcacc ttggtataac caaaaagaac
    12181 gtgaacgtat gcaaaaaatg gaagaagaat actacaacgc acaacaacac gcataaaaaa
    12241 actgagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
    12301 gcggaatcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    12361 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctattttt
    12421 tatagtaatt ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac
    12481 ttctgataat attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc
    12541 catttgtcga ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat
    12601 ttttcttttg agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta
    12661 cgcaatactt ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa gaacccttct
    12721 gtaaaaccat agcgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat
    12781 cgttggtttt tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt
    12841 gtctttctca acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct
    12901 tcatatacga tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct
    12961 tctacatcct ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc
    13021 ttttttgact gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga
    13081 taatccgcta tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat
    13141 tttatatccg gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat
    13201 ttttgccata gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct
    13261 aaggccagat caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca
    13321 tgcaggtgat cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat
    13381 ttaacgatgt attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt
    13441 cgttttgttc gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct
    13501 aaataatgtt cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt
    13561 tcccgctgtt ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt
    13621 ttagaaattt tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg
    13681 gattgccatt cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac
    13741 ataacgttag caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg
    13801 gaaatatctt ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg
    13861 tcttctaaag acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat
    13921 ggcacgtgga tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa
    13981 cgatacatga tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga
    14041 ccatgatctt gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct
    14101 ggaccgtttt cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt
    14161 gcttctgcta gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt
    14221 ccgacacaga tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg
    14281 cttttttcga atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct
    14341 ggcatttttt ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa
    14401 cctgttacta gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc
    14461 ttttatcata ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat
    14521 aacagatcaa acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga
    14581 ggttgcgaca attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa
    14641 gcaatttgga aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat
    14701 ttgtctttca tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc
    14761 agcagggtcc cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct
    14821 ccatgactaa atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat
    14881 gcattcccca tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg
    14941 ttcacatttc cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca
    15001 gcgcttgtaa ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct
    15061 actgtgccca gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa
    15121 aagaacatca agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct
    15181 aaattggtaa atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa
    15241 attttcattc ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt
    15301 tctcctttgc tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg
    15361 ttcacgattg ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa
    15421 aaggctgacc cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa
    15481 atcaatacga gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata
    15541 tcaggatttg taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa
    15601 tttcttttct ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg
    15661 aacttccact aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga
    15721 ttggactagc aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac
    15781 attttccata gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa
    15841 cccttttgat tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc
    15901 aactccacca aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc
    15961 tacaatcata ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt
    16021 cagaccattt ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc
    16081 gcgattgttt acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc
    16141 agtcaaagct gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc
    16201 aaacccgaga ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc
    16261 tccataaaat gacaatattt ttttaattat aaagcaaaac acataaaaaa tatagttgtt
    16321 ttctcacgaa atagacacta tttttaaata tttttcgtta tttttttatc acaaaaatta
    16381 tcttacttat tgtaaggtaa agcttttttt aatttattgc ccgataatat atagaaataa
    16441 ggtctagtta ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc
    16501 aaagcattca tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta
    16561 aaaagtatga gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacaa
    16621 tctgccaccg ggtaggataa agaggatgat cattccatta ggtctttgaa gggatgattt
    16681 tcctcttttt gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat
    16741 taatcatagc aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt
    16801 tttctaaaat caattattct ttttacacag tgattggcat gattgctagt ttttactttt
    16861 tgtcaaaggc aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg
    16921 gcgcagttgg ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca
    16981 cttggttctt tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc
    17041 actagacact tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat
    17101 ttttttatga aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt
    17161 gtatgcttgt actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag
    17221 aattatccaa gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc
    17281 ctggcaaaat cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag
    17341 ctattttagg actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt
    17401 ctccctctag tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct
    17461 atccattctt aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg
    17521 ctatcgacac tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat
    17581 cctactcgtt gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt
    17641 ctatcgttat tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc
    17701 gaaaaatcat taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac
    17761 tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca
    17821 aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa
    17881 caaatgatct agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg
    17941 ataaaatcaa gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt
    18001 ctgcacaaaa tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc
    18061 tattggaaat tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca
    18121 agaattattt aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt
    18181 tttgctcata ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga
    18241 aatgactgct cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc
    18301 aagcacgatc atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa
    18361 gtattctcga ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta
    18421 tttatacctt ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat
    18481 ttccttcacg gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac
    18541 tttgggtagt tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt
    18601 tatcaatagt tccggcgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat
    18661 catatctggt atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat
    18721 cagtatgttg aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca
    18781 tctttctact aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt
    18841 aggatatctc gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa
    18901 agagacgttc agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag
    18961 gagctgtgac aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag
    19021 gctcacaacc tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa
    19081 ataaaaccat tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt
    19141 ggatatgttg tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg
    19201 gcaagaaaag aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata
    19261 cttgaaaggc acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag
    19321 aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa
    19381 gtgtgtaagc aaagttataa gcactgtcag tgaataataa tgaaagtaaa aagacattcg
    19441 tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc
    19501 atgctttagg caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg
    19561 tccatgagag ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc
    19621 cccatttacc aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt
    19681 gcagctgatt catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa
    19741 tcaagccgaa tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg
    19801 aacctacgac agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac
    19861 tcaatacttg actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag
    19921 aaaagttatc aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa
    19981 ataatggcac taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc
    20041 cccgattcac tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca
    20101 gattttgccc accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg
    20161 caaaagctac gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag
    20221 cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt
    20281 ttttattgat attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc
    20341 aactgaaaat agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc
    20401 cacctccaat cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt
    20461 tctgttgctc tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta
    20521 taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac
    20581 cggctttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa
    20641 aaacttaaaa aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt
    20701 atatatatcg atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga
    20761 aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa
    20821 tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg
    20881 cggaaatggt ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct
    20941 ggaacatgaa gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt
    21001 caataatcag tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt
    21061 ggcagattat gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc
    21121 agaaaacaat aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg
    21181 tttctattgt ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct
    21241 ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc
    21301 atggatgttt ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta
    21361 tcaggtgtca cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca
    21421 actgtgttga ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata
    21481 gcgtgtttcg ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg
    21541 ttttctggtt ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt
    21601 gctttatcta aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca
    21661 gctgaagttt gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct
    21721 aattcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca
    21781 ccagcataag ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat
    21841 gggatcaaag cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg
    21901 atcactgccc cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt
    21961 aatgttactc cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct
    22021 ttgatcattt ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca
    22081 cccacttgtc ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca
    22141 tcaatcattt gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc
    22201 aagaataatg tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg
    22261 atcccaccga ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca
    22321 atcagaccga tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac cgcatcaggt
    22381 actactttat gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct
    22441 aggatcccac cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct
    22501 tccggattca tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt
    22561 gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg
    22621 tttttcaaga tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg
    22681 gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat
    22741 gcaggaatca ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat
    22801 ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct
    22861 tttccagatg ctgatgaatt tgctgtagct gtacttacag aagaaagttt ttctcctaat
    22921 ttcacgcctg ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg
    22981 atgacttgaa gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca
    23041 gagacattta caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca
    23101 atgctttcta cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc
    23161 aaagtcagtt cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc
    23221 gtttgcttca gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac
    23281 gatagccagc ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc
    23341 agcttctgtc gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg
    23401 tttgacatcg accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga
    23461 aatcatattc aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa
    23521 aaattctgga cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg
    23581 tttattgcaa gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc
    23641 gatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa
    23701 attcagcttg attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc
    23761 tacagtcatt gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc
    23821 gaatgtcggt acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc
    23881 catataaatc agacgtccac cttttttaaa ggactcaatc gtgttagcaa tcactggttc
    23941 gatacttgtc agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag
    24001 ttgaacggct tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc
    24061 cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat
    24121 tgatagttca taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta
    24181 gctactacat tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc
    24241 atatagcgca aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta
    24301 tttcttgctg ggataggcgg aatctctcga aaacgtgcat ctacgcttcg tatgacatct
    24361 cttgcatcgt cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct
    24421 acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga
    24481 acgttttctg acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga
    24541 tctagagcgc ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga
    24601 tatagtgggc ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga
    24661 attttgagcc attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt
    24721 ggataataat tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca
    24781 tcgatatctt taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag
    24841 tcggcaatct gctgattgga aatatgataa tccattctta aacctgttac accaattgct
    24901 ttcaaaggcg ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata
    24961 tcgaccatca attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt
    25021 tgatatgccg aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca
    25081 ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat
    25141 attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt ctatgactag
    25201 agtatatatc taaagaaaca aaatttcaat acttttatta aaataattaa atttcatttc
    25261 taaaggttga gtttatatta tcaaaagaat tgtcataacc taaaaaaggc actgctaagc
    25321 aaaagcagtg ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa
    25381 accaattctg tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa
    25441 taagcattga tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg
    25501 tcttcgatgc attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc
    25561 ccatttggga atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt
    25621 ggcagtccat cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc
    25681 aagctgagac gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg
    25741 atgattttcg tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc
    25801 atctgtcctt tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc
    25861 caagttgttt ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag
    25921 ttccccttaa ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa
    25981 ataatctcag tagatacttt atccaggaag gtttgatcat gttccactag taacatggca
    26041 ggtttcacgt tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc
    26101 aatggttcat cccatgtata tagttcagct ggctgtgata aagatttagc caattccact
    26161 tttttccgtt ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc
    26221 ccaagctttc ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat
    26281 tccgccaacg ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt
    26341 tttggctggt atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata
    26401 atggatgact ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt
    26461 gtaaaatgaa ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt
    26521 aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata
    26581 tcttttaata gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg
    26641 gatcgtttca tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat
    26701 tcagtatcga tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat
    26761 tcggcttttt cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt
    26821 tgagccattt caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct
    26881 tgataaagtt ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata
    26941 aatccccggt catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct
    27001 acttgttttc taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga
    27061 gtgtcatcca caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg
    27121 ctgaattctc tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt
    27181 tcccatatct caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct
    27241 tttgtctgtt gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt
    27301 agtttgtttt gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt
    27361 cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg
    27421 ctgtcgtagc cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta
    27481 aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac
    27541 aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt
    27601 gtctccacaa ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac
    27661 ggatccctca ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca
    27721 actacttcgg gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt
    27781 ctaatgtttg cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta
    27841 agacaattgg gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag
    27901 cgattattgg ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt
    27961 ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct
    28021 ttttttccaa tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca
    28081 acaaaaaaag ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat
    28141 ccacctccca gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag
    28201 ctagcgtcgt atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac
    28261 ctgtatatgg attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt
    28321 ttcttcgatc aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt
    28381 ctaaagaagc acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc
    28441 ctaaagtgac aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta
    28501 aggcataact ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc
    28561 ccaatgcttg tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat
    28621 agctgatttt tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc
    28681 tgagcgcgta atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga
    28741 tttttcttcc tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag
    28801 aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc
    28861 ttcttaagta agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat
    28921 ttttctatat actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca
    28981 atagctgggg ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat
    29041 gaggtcctct tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg
    29101 attgctgact ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa
    29161 atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta
    29221 gatgatatgt tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta
    29281 ctaaatggat taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta
    29341 tttgggatca cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt
    29401 gatggagaaa tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt
    29461 gtagaagtct ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc
    29521 atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt
    29581 aatattgctg aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct
    29641 gaatttgctt ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa
    29701 catattgctt ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa
    29761 gacctgatca aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga
    29821 aaaggacaaa aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat
    29881 gcaaaagaag gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca
    29941 gcagctggtt ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg
    30001 atgccataag aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt
    30061 tatttgtttt caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta
    30121 gtgataaatc gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt
    30181 ggcttaaatg gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa
    30241 aaaatatttt caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt
    30301 ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc
    30361 aacagttcca gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga
    30421 tatccaggtt gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt
    30481 tttataaatt cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca
    30541 aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa
    30601 ctattatgtt tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg
    30661 attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc
    30721 caaaaaaata acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata
    30781 tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat
    30841 ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa
    30901 ggcaaaaaat aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt
    30961 tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca
    31021 gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata
    31081 aatcattctt ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa
    31141 tttgttatca ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt
    31201 gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc
    31261 agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt
    31321 ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg
    31381 attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat
    31441 cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat
    31501 tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc
    31561 tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt
    31621 ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg
    31681 gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt
    31741 cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg
    31801 tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac
    31861 ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt
    31921 cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt
    31981 ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg
    32041 atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc
    32101 cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc
    32161 cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta
    32221 ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat
    32281 tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg
    32341 atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg
    32401 gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg
    32461 ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc
    32521 agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt
    32581 ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt
    32641 gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa
    32701 accctctgtg caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat
    32761 gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt
    32821 ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt
    32881 cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat
    32941 gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc
    33001 tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga
    33061 cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg
    33121 agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga
    33181 agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca
    33241 aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc
    33301 tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    33361 caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc
    33421 ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag
    33481 acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc
    33541 tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa
    33601 aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg
    33661 gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt
    33721 tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa
    33781 tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa
    33841 actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa
    33901 acttccggca attccccaag gcactaagta gttgattact gttccaccat cttctagcgt
    33961 tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc
    34021 aggcagaata acagatagga attgttcacc aataaaaata tttactccga tacttgtcat
    34081 caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc gttccatcac
    34141 ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa
    34201 tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat cgatttgttg
    34261 gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt
    34321 ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgttaa
    34381 aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca
    34441 gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact
    34501 tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg
    34561 gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc
    34621 tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac
    34681 aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc
    34741 aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca
    34801 cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat
    34861 tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa
    34921 aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc
    34981 tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt
    35041 gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat
    35101 taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc
    35161 ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc
    35221 atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact
    35281 ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat
    35341 gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact
    35401 ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga agtttacctt
    35461 ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt
    35521 tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt gtttttttct
    35581 tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag
    35641 atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc
    35701 acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc
    35761 taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag
    35821 acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg
    35881 gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa
    35941 tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt
    36001 ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc
    36061 gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac
    36121 atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc
    36181 caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt
    36241 gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg
    36301 gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc
    36361 tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt
    36421 ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac caaacaaatt
    36481 atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt
    36541 gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc
    36601 tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa
    36661 ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg
    36721 taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa
    36781 ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct
    36841 tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac
    36901 tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga tattcacata
    36961 aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc
    37021 agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc
    37081 tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcga taatatcaga
    37141 aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa
    37201 cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt
    37261 gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt
    37321 tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg
    37381 ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt gattgtaatc
    37441 tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc
    37501 ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt ttttgtcagt
    37561 tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac
    37621 tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa
    37681 tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca
    37741 caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc
    37801 cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg
    37861 ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa atgataccga
    37921 ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg
    37981 cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg
    38041 ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca
    38101 agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca
    38161 gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc
    38221 caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt
    38281 cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca
    38341 ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt ttgtaaaata
    38401 tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg tttggtttct
    38461 ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca
    38521 tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc
    38581 gtctgatttc caatataact taattccacc agccatttat tggcgatctg atcggcaaaa
    38641 gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa
    38701 tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc tttttcaacg
    38761 attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca
    38821 tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg
    38881 atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta
    38941 ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt
    39001 ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt
    39061 tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa
    39121 atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct
    39181 tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat gacttctcct
    39241 gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag
    39301 taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc
    39361 tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa
    39421 attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac
    39481 gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag
    39541 tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg
    39601 gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa
    39661 gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa
    39721 aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc
    39781 ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt
    39841 tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat
    39901 tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa
    39961 cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa
    40021 actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt ctgttttttt
    40081 atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc
    40141 cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa
    40201 tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact
    40261 agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc
    40321 cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa agtgatccaa
    40381 gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc
    40441 ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag
    40501 ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc
    40561 tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac
    40621 gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata
    40681 cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac
    40741 attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa agaacagaag
    40801 caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata gattttcttc
    40861 acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg
    40921 ccaggaagtg gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg
    40981 acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct ttggataaat
    41041 ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca
    41101 aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc caatgtatgg
    41161 aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta agtagtaata
    41221 tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact agatgcattc
    41281 catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag
    41341 gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg taaaaatggc
    41401 aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc
    41461 ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa
    41521 atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct
    41581 gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct
    41641 cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc
    41701 tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca
    41761 ctcatttccg agattttctc accggtaacc aattcattga tctctagacc tacttgacgg
    41821 cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt
    41881 tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga
    41941 taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga
    42001 ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt
    42061 gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata
    42121 aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca
    42181 gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc
    42241 actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc
    42301 acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa
    42361 acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt
    42421 gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt ctggatggca
    42481 ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc
    42541 gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa
    42601 agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc
    42661 atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt
    42721 gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca
    42781 acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat aaagacaaga
    42841 ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact
    42901 ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa
    42961 cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt
    43021 tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca
    43081 agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc
    43141 ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt atctccagga
    43201 gctgctaata catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta
    43261 aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt gccatatgaa
    43321 atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt
    43381 gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag
    43441 attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc
    43501 tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg
    43561 ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt tttcgaatga
    43621 ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga cttccaatca
    43681 tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca ccgatgggga
    43741 tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt
    43801 aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat
    43861 acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg
    43921 cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc
    43981 ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc
    44041 atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt
    44101 tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg
    44161 gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc
    44221 atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact
    44281 cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg
    44341 atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc
    44401 gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc aggtacgatc
    44461 cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat
    44521 gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa
    44581 aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc
    44641 cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt
    44701 gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg
    44761 ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat gtgataagct
    44821 ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa
    44881 cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa
    44941 tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat
    45001 ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa
    45061 gtattcttca atgattcggc attcataacg taaaaattca aattctgtct cagatacttc
    45121 tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg ttttcttaaa
    45181 atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc
    45241 tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc
    45301 atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat
    45361 ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga ttccttccgc
    45421 taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata
    45481 cataatagtt ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa
    45541 aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa
    45601 gcattagaat gttgtctaac tactgaaggt tggtttgatt gtttattgat tatttttttg
    45661 ctgttcaacg tctgttcttg ttactggata taagttggtt gttgttttat taccattttt
    45721 tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg attgcttcat tacgttccgc
    45781 ttcattttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg gcacataaaa
    45841 ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc
    45901 ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga tttctcctgt
    45961 ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa agttcccatt
    46021 tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa aatgaagata
    46081 atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc
    46141 gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca atggcactcg
    46201 ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcgcttgc
    46261 taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga
    46321 attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag
    46381 ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa
    46441 gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt
    46501 gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata aatctttatc
    46561 taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat caaagaaatt
    46621 gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta cattcccatg
    46681 gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg cttgaaagac
    46741 attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc
    46801 caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa
    46861 gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt cttgtccgtc
    46921 taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga tcacattttt
    46981 ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct ttgtataatc
    47041 taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag tcattcctgt
    47101 tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa tatatgaacg
    47161 gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta gatttcctga
    47221 aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga cagggttgct
    47281 tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc
    47341 cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg acaatccttg
    47401 cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata
    47461 aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatagc
    47521 taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc
    47581 aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc
    47641 tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc
    47701 taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca
    47761 ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat
    47821 ttttttattc tttctaaaga ttattccgtc cacttttcac agcataaaac cgctgcgatg
    47881 taagcgatta aactaatgaa gagataaaag tcttgtggtg accgcttcac tccacggtat
    47941 tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa ttcttgtcgc
    48001 agctccttat ttcttgatgc aggacagctt tttcacaact tcttccttaa acggtgttct
    48061 cctgccaact cctcggccac aagtcacaat atttataaaa catgagaagc tgttttttga
    48121 aattgttcct tgtggctcag agttaaacgg caggctcaag acctcttgat acacggtgtt
    48181 caatcagctg accctcggta ttagattatg tcaggacgct aattttacaa cctcttggta
    48241 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatataagga
    48301 gctatttttt gaaattgctc cttgtggctc agagttaaac ggcaggctca agacctcttg
    48361 atacacggtg ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg
    48421 aactattttg ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctg
    48481 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
    48541 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
    48601 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
    48661 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
    48721 tcacttttcc atttttcaaa cgggctactt tgtcgccaac atgagtcaaa agcaaaacgg
    48781 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
    48841 tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt
    48901 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
    48961 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
    49021 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
    49081 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
    49141 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
    49201 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
    49261 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt
    49321 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
    49381 atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt
    49441 ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg
    49501 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
    49561 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
    49621 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
    49681 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
    49741 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
    49801 ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac
    49861 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
    49921 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
    49981 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
    50041 agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt
    50101 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
    50161 tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt
    50221 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
    50281 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
    50341 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
    50401 aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
    50461 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
    50521 atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
    50581 atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
    50641 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
    50701 tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt
    50761 tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga
    50821 tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg
    50881 caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca
    50941 tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt
    51001 aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
    51061 tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
    51121 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
    51181 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct ggacaattca
    51241 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
    51301 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
    51361 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
    51421 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
    51481 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
    51541 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
    51601 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg
    51661 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
    51721 tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
    51781 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
    51841 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg
    51901 gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
    51961 tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc
    52021 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
    52081 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
    52141 ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa
    52201 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
    52261 aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa
    52321 cagtgctgac agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag
    52381 aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg
    52441 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
    52501 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
    52561 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
    52621 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
    52681 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
    52741 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
    52801 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
    52861 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
    52921 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
    52981 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
    53041 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
    53101 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
    53161 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
    53221 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
    53281 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
    53341 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
    53401 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
    53461 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
    53521 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
    53581 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
    53641 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
    53701 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
    53761 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
    53821 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
    53881 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
    53941 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
    54001 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
    54061 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
    54121 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
    54181 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
    54241 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
    54301 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
    54361 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
    54421 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
    54481 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
    54541 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
    54601 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
    54661 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
    54721 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gt
//
LOCUS       DAJSVD010000005        52019 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSVD010000005 DAJSVD010000000
VERSION     DAJSVD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.005"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            278..649
                     /locus_tag="ON498_000221"
     CDS             278..649
                     /locus_tag="ON498_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3938524.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(923..1615)
                     /locus_tag="ON498_000222"
     CDS             complement(923..1615)
                     /locus_tag="ON498_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3938525.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1618..2403)
                     /locus_tag="ON498_000223"
     CDS             complement(1618..2403)
                     /locus_tag="ON498_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3938526.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2640..4040
                     /gene="lacG"
                     /locus_tag="ON498_000224"
     CDS             2640..4040
                     /gene="lacG"
                     /locus_tag="ON498_000224"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3938527.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4065..4382
                     /locus_tag="ON498_000225"
     CDS             4065..4382
                     /locus_tag="ON498_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3938528.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4395..6068
                     /locus_tag="ON498_000226"
     CDS             4395..6068
                     /locus_tag="ON498_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3938529.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6082..7713
                     /locus_tag="ON498_000227"
     CDS             6082..7713
                     /locus_tag="ON498_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR3938530.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8204..9769)
                     /gene="guaA"
                     /locus_tag="ON498_000228"
     CDS             complement(8204..9769)
                     /gene="guaA"
                     /locus_tag="ON498_000228"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3938531.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9828..10751)
                     /locus_tag="ON498_000229"
     CDS             complement(9828..10751)
                     /locus_tag="ON498_000229"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3938532.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10947..11552
                     /locus_tag="ON498_000230"
     CDS             10947..11552
                     /locus_tag="ON498_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3938533.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11860..12876
                     /locus_tag="ON498_000231"
     CDS             11860..12876
                     /locus_tag="ON498_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3938534.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13017..14318
                     /locus_tag="ON498_000232"
     CDS             13017..14318
                     /locus_tag="ON498_000232"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3938535.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14364..15026
                     /gene="deoC"
                     /locus_tag="ON498_000233"
     CDS             14364..15026
                     /gene="deoC"
                     /locus_tag="ON498_000233"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3938536.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15047..15445
                     /locus_tag="ON498_000234"
     CDS             15047..15445
                     /locus_tag="ON498_000234"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3938537.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15511..16593
                     /locus_tag="ON498_000235"
     CDS             15511..16593
                     /locus_tag="ON498_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3938538.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16805..18367
                     /locus_tag="ON498_000236"
     CDS             16805..18367
                     /locus_tag="ON498_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3938539.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18360..19493
                     /locus_tag="ON498_000237"
     CDS             18360..19493
                     /locus_tag="ON498_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938540.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19493..20449
                     /locus_tag="ON498_000238"
     CDS             19493..20449
                     /locus_tag="ON498_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938541.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20548..21714
                     /gene="deoB"
                     /locus_tag="ON498_000239"
     CDS             20548..21714
                     /gene="deoB"
                     /locus_tag="ON498_000239"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3938542.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21738..22556
                     /locus_tag="ON498_000240"
     CDS             21738..22556
                     /locus_tag="ON498_000240"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3938543.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22573..23280
                     /gene="deoD"
                     /locus_tag="ON498_000241"
     CDS             22573..23280
                     /gene="deoD"
                     /locus_tag="ON498_000241"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3938544.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23578..24264)
                     /gene="gpmA"
                     /locus_tag="ON498_000242"
     CDS             complement(23578..24264)
                     /gene="gpmA"
                     /locus_tag="ON498_000242"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3938545.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24436..25017)
                     /locus_tag="ON498_000243"
     CDS             complement(24436..25017)
                     /locus_tag="ON498_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3938546.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25150..25833)
                     /gene="rpiA"
                     /locus_tag="ON498_000244"
     CDS             complement(25150..25833)
                     /gene="rpiA"
                     /locus_tag="ON498_000244"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3938547.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26152..26565
                     /gene="rpsL"
                     /locus_tag="ON498_000245"
     CDS             26152..26565
                     /gene="rpsL"
                     /locus_tag="ON498_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3938548.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26649..27119
                     /gene="rpsG"
                     /locus_tag="ON498_000246"
     CDS             26649..27119
                     /gene="rpsG"
                     /locus_tag="ON498_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3938549.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27213..29300
                     /gene="fusA"
                     /locus_tag="ON498_000247"
     CDS             27213..29300
                     /gene="fusA"
                     /locus_tag="ON498_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3938550.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29472..30659
                     /gene="tuf"
                     /locus_tag="ON498_000248"
     CDS             29472..30659
                     /gene="tuf"
                     /locus_tag="ON498_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3938551.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31066..31374
                     /gene="rpsJ"
                     /locus_tag="ON498_000249"
     CDS             31066..31374
                     /gene="rpsJ"
                     /locus_tag="ON498_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3938552.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31405..32034
                     /gene="rplC"
                     /locus_tag="ON498_000250"
     CDS             31405..32034
                     /gene="rplC"
                     /locus_tag="ON498_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3938553.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32062..32685
                     /gene="rplD"
                     /locus_tag="ON498_000251"
     CDS             32062..32685
                     /gene="rplD"
                     /locus_tag="ON498_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3938554.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32685..32975
                     /gene="rplW"
                     /locus_tag="ON498_000252"
     CDS             32685..32975
                     /gene="rplW"
                     /locus_tag="ON498_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3938555.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33014..33847
                     /gene="rplB"
                     /locus_tag="ON498_000253"
     CDS             33014..33847
                     /gene="rplB"
                     /locus_tag="ON498_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3938556.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33890..34168
                     /gene="rpsS"
                     /locus_tag="ON498_000254"
     CDS             33890..34168
                     /gene="rpsS"
                     /locus_tag="ON498_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3938557.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34191..34538
                     /gene="rplV"
                     /locus_tag="ON498_000255"
     CDS             34191..34538
                     /gene="rplV"
                     /locus_tag="ON498_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3938558.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34552..35208
                     /gene="rpsC"
                     /locus_tag="ON498_000256"
     CDS             34552..35208
                     /gene="rpsC"
                     /locus_tag="ON498_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3938559.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35211..35645
                     /gene="rplP"
                     /locus_tag="ON498_000257"
     CDS             35211..35645
                     /gene="rplP"
                     /locus_tag="ON498_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3938560.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35635..35823
                     /gene="rpmC"
                     /locus_tag="ON498_000258"
     CDS             35635..35823
                     /gene="rpmC"
                     /locus_tag="ON498_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3938561.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35848..36114
                     /gene="rpsQ"
                     /locus_tag="ON498_000259"
     CDS             35848..36114
                     /gene="rpsQ"
                     /locus_tag="ON498_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3938562.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36172..36540
                     /gene="rplN"
                     /locus_tag="ON498_000260"
     CDS             36172..36540
                     /gene="rplN"
                     /locus_tag="ON498_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3938563.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36577..36885
                     /gene="rplX"
                     /locus_tag="ON498_000261"
     CDS             36577..36885
                     /gene="rplX"
                     /locus_tag="ON498_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3938564.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36913..37452
                     /gene="rplE"
                     /locus_tag="ON498_000262"
     CDS             36913..37452
                     /gene="rplE"
                     /locus_tag="ON498_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3938565.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37471..37656
                     /locus_tag="ON498_000263"
     CDS             37471..37656
                     /locus_tag="ON498_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR3938566.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37692..38090
                     /gene="rpsH"
                     /locus_tag="ON498_000264"
     CDS             37692..38090
                     /gene="rpsH"
                     /locus_tag="ON498_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3938567.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38122..38658
                     /gene="rplF"
                     /locus_tag="ON498_000265"
     CDS             38122..38658
                     /gene="rplF"
                     /locus_tag="ON498_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3938568.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38819..39175
                     /gene="rplR"
                     /locus_tag="ON498_000266"
     CDS             38819..39175
                     /gene="rplR"
                     /locus_tag="ON498_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3938569.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39196..39696
                     /gene="rpsE"
                     /locus_tag="ON498_000267"
     CDS             39196..39696
                     /gene="rpsE"
                     /locus_tag="ON498_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3938570.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39711..39890
                     /gene="rpmD"
                     /locus_tag="ON498_000268"
     CDS             39711..39890
                     /gene="rpmD"
                     /locus_tag="ON498_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3938571.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39934..40374
                     /gene="rplO"
                     /locus_tag="ON498_000269"
     CDS             39934..40374
                     /gene="rplO"
                     /locus_tag="ON498_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3938572.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40374..41669
                     /gene="secY"
                     /locus_tag="ON498_000270"
     CDS             40374..41669
                     /gene="secY"
                     /locus_tag="ON498_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3938573.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41731..42378
                     /locus_tag="ON498_000271"
     CDS             41731..42378
                     /locus_tag="ON498_000271"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3938574.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42569..42787
                     /gene="infA"
                     /locus_tag="ON498_000272"
     CDS             42569..42787
                     /gene="infA"
                     /locus_tag="ON498_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3938575.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42820..42936
                     /gene="rpmJ"
                     /locus_tag="ON498_000273"
     CDS             42820..42936
                     /gene="rpmJ"
                     /locus_tag="ON498_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3938576.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42954..43319
                     /gene="rpsM"
                     /locus_tag="ON498_000274"
     CDS             42954..43319
                     /gene="rpsM"
                     /locus_tag="ON498_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3938577.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43347..43736
                     /gene="rpsK"
                     /locus_tag="ON498_000275"
     CDS             43347..43736
                     /gene="rpsK"
                     /locus_tag="ON498_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3938578.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43816..44754
                     /locus_tag="ON498_000276"
     CDS             43816..44754
                     /locus_tag="ON498_000276"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3938579.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44782..45162
                     /gene="rplQ"
                     /locus_tag="ON498_000277"
     CDS             44782..45162
                     /gene="rplQ"
                     /locus_tag="ON498_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3938580.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45434..46273
                     /locus_tag="ON498_000278"
     CDS             45434..46273
                     /locus_tag="ON498_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3938581.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46249..47118
                     /locus_tag="ON498_000279"
     CDS             46249..47118
                     /locus_tag="ON498_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3938582.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47108..47905
                     /locus_tag="ON498_000280"
     CDS             47108..47905
                     /locus_tag="ON498_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3938583.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48108..48854
                     /gene="truA"
                     /locus_tag="ON498_000281"
     CDS             48108..48854
                     /gene="truA"
                     /locus_tag="ON498_000281"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3938584.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49309..51978)
                     /locus_tag="ON498_000282"
     CDS             complement(49309..51978)
                     /locus_tag="ON498_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR3938585.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        16495 a         9535 c        11317 g        14672 t
ORIGIN      
        1 ttgtatattt agtaagtagc tacacacttc tatataaatt cagaagttct tctagagttt
       61 ctaaagtttg tttcttaatt agcttattga ttctataata tttttgattt atataggaaa
      121 ttaaaaatat tatagaatca acactcagag aacgggttgg tttttaatag atttaataag
      181 tactgataat gaaatttctc ctggttggga aaacgaagtt ggttaccctg tagaagaatt
      241 ttttcaagat tactttgcgg agtatgccga taggataatg agagattacg taacagataa
      301 aactcaagaa gtcatcaatc aatcccagtc attaaatgaa ttatctgata aggcgaaaga
      361 agaagcagtt gccattattt cttcagggat tagtgactat attgatatga gtggtgaaat
      421 caattatggc gataaagaca aaattgtacg catgataaaa gcttttatca aaacttttga
      481 tgtgaaggca gatcttaagt ttaatgatac ttacctagta ggaaaattaa ttaatatcct
      541 tgggacagag aaagatacgg aaacgtttat ttcctctttg tctatgttgt ttacagataa
      601 gcatttttca actcagcttg gtggagagga actagtggaa atcttttaac cattacgccc
      661 agcattaata aaagtatatc aaaaaagtac tcctgatgat ttttaagatt ggtttgagaa
      721 ataatgtgaa aacactattt ttaatgttaa ttatagatag agatgaacgc gtcatatggt
      781 taaaagaatg aaaaagtcaa agatacactg ctatatttca ctaaggatat agcagtgtat
      841 ctttcatgtc gtttcactgg cagtttttta taaaaggagg tgtgcatttc agttgctttt
      901 ctttggtagt tagttgaatc gtttaagaat taacggatgt ggcagacagg tgctctgtcc
      961 aaaaaccagt aaaatctttg atggttccat ctttatttag ctgtgcctgt tcaaatactg
     1021 ttttgttttt accgaatgga tcaattgcaa taattgttcc gatccccgca cgtttggcag
     1081 caagtaatcc tgagtaagca tcttcgataa caagacaatc agccggagct aagctcagtt
     1141 tgtctgccgc tttaagaaaa atatccggtt gcggtttccc agggaaagag ccgtcgtcat
     1201 agacaatctt gtcccagtca aaccagcggg ctaaatcgaa aatatcaaaa taaaaagcaa
     1261 cattctcttt gacagtagcc gtggcaatcg ttaatggaat ctgttggtca gtcagctggt
     1321 caagagtcgc ttcaagccct tttgtcaact gaagctcgtt ttcattttgc aagcaaagca
     1381 tacgataata gctttctttt tcttctgaca atacggcgat ctcagaggcg gtcaaatgct
     1441 ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc atgaatattt tttaaaatat
     1501 cctcgtctga taaaccatct tttgtatgat catgtatcat atgcagccaa gcagcttcat
     1561 gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa tataatccct ttcatagtta
     1621 cgctcctata gtggtatttt cggttgaatc agcaatttgc agatcgacat attgtgcata
     1681 gtgctcttga acctcccgcg agatctggcg atccgtaatg agcagatcga aatcatgcaa
     1741 attataaaac acataaaaat catctcgatt gaatttttta ttatcaacga gtagatattt
     1801 cttacgggaa ttatttagag cgatctgctg tgcttctcct tcttcggtac tgtaagtaga
     1861 gatcgcttcg ttgtgaacgc catttgaact gataaatgct tttgtaaact ttagtcttct
     1921 taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta tttttccgga aatcaccgcc
     1981 aatcagtaga atttctggtc catctgcttg agcaagaata tcaaaaactg gataattatt
     2041 tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc aatagttcga ttgttgtacc
     2101 tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag gctgctgtgg cagcaatttc
     2161 ttttttttct tgaatctgca gagtggcttt ttcagtgtga gaaagttctt gatctaaagt
     2221 gtaggaaaga ctttgagccc ctccatggac acgcagcagc ttgcccgatt tttctaattc
     2281 atctaaatct ctgcgaacgg tcatatcaga aacatctaat tgattgatga tttcattaac
     2341 ggtaaggatt ccttttttat tgaccatttc tacgatagta agcaatcttt cttttttcaa
     2401 cattgtcttc acctctaatt taaggataat ctatcatata aacggacaac agtcaatcta
     2461 attcgaacaa aagtaaacat ttagggtggt ttttatgttt gttacaatag attttattgc
     2521 tgttttctgg tttttatgat tatttgtgtt tgtttttgtt gacaaatgtt ttggatgcgt
     2581 ttacaatgca atcagttaga aagagcgaaa actgattata gatttgagga gagatgtaca
     2641 tgcttaaatt accagaagat tttatttttg gcggagccac agcagcctat caggttgaag
     2701 gagccactaa ggaaggtggc aaaggcgctg ttgcttggga tgacttttta gaggaacaag
     2761 ggcgattcag tcccgatccg gcaagtgatt tttatcatca atatgcaaag gacattgaat
     2821 tgtgtgaacg ttttggtgta aatggtctaa gattatccat tgcttggagc cgcatttttc
     2881 ctgatggagc tgggaaacca aatccagaag gaatcgcttt ttatcatcgt gtgttcgaag
     2941 aatgtaagaa aagaaacgta acgccgtttg tgacgttgca tcattttgat acccctaaac
     3001 gtttgtttga ccatggagat tttttgaatc gagaaacaat agaagcattt gtttcctatg
     3061 ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg gagtaccttt aatgaaatct
     3121 atccagttgc aaccaatcaa tatttgttag gtgttttccc accaggaatc aaatatgatt
     3181 ttacaaagat cgtcgcctgt cttcataata tgatggttgc tcatgcccgt gtagtgaact
     3241 atttcaaaga aaacgaactt ccgggtgaaa ttggtgtagt tcactcatta gagacgaaat
     3301 atgcggcaac agacgcgccg gaagataagc atgctgcctt tttagacgat gcattgtcga
     3361 tccgtttctt attagatgct acctatttag gctattattc aactgaaacc ttgactgctc
     3421 ttgatgagat atgtgaagca aatcaggcca gctatcattt tcctgaagaa gatttcgttg
     3481 aattgaaaaa agcatccacg cgtaatgact acttagggat caatcactac caatgccatt
     3541 ttgtcaaagc ttacgatgga gaaaatgcaa tccatcataa cggtacgggt gaaaaaggga
     3601 cctctgttta taaagtgaag ggaatcggag aacgtattta taaagaagga attcctcgaa
     3661 ctgattggga ctggctgatt tatccagaag gtctatatga cttgttgctg cgaattaaaa
     3721 gcgattaccc acattacaac aaaatctata tcactgagaa cgggatgggg tataaagatc
     3781 agtttgaaga tggcattatt atggatcagc cgagaatcga ctatttgaga gtctatttgg
     3841 aatccttaag caaggcgata acagccgggg taaatgtgaa ggggtatttt ttgtggtcgc
     3901 ttatggatct tttctcatgg acaaatgggt ataacaaaag gtatggcttg ttttatgttg
     3961 attttgaaac ccagaagcgt tacccaaaag aatcagctta ctggtacaag ttggtcagtg
     4021 aaaccaaaac gattatttaa taagtgataa tggaggaaga aaagatgaat aaagaggaat
     4081 tacaaatgct tggttttgag atcgttgcat attcaggcga cgctcgcagt acattattga
     4141 aattattgaa agaagtaaga caaggaaact ttgaacatgt tgattcggcg ttgaaagaag
     4201 ccgacgaaaa tttaaccctt gcacataatt cgcaaacaaa aattttagcg gaagaagcat
     4261 ctgggaaaga gatggagatg ggattcattt ttatccatgg gcaggatcat ttaatgacca
     4321 ctttattgct gagagatctg attcaagact ttattgtact gtacagacag aatcaaggat
     4381 aggagaagaa cgctatggat ggaatcatta aacaaatcga aaaagggaaa ccattttttg
     4441 aaaaaatctc acgtaatagt tatttaggag ccgttcgtga cggattttta acagcgatgc
     4501 cagctatttt attttcaagc attttcattt tgattgctgc ggttcctgag atttttggtt
     4561 accaatggcc tgaatctgtc tccacatggc tatggaaagt ctataattat tcgatgggtg
     4621 ttgtagggtt actcgtttca gcaaccactg ctcgctgctt ggctgattcg atgaatcgtc
     4681 gcttaccgaa aaacaaagta atcaacacca cctctgtgat gctggcctct gtggtaagtt
     4741 tcctgttgtt aagcgtctct ccgattgaag gtggattttc aacggaatat atggggacaa
     4801 agggactact ggcttccttt gtcgctgcat ttttaacagt taatttatat aagttttgtg
     4861 tgctgaaaga tattaccatt aaaatgccta aagaagttcc tggaacgatt tcgcaaacct
     4921 tccgagatat ttttcctttc tctttttcag tatttgcggc cgttttaatt gatttaggca
     4981 ttcgacaaat cttccaaatc tcatttgcag aagcaatcat tacggtgctg cagccgctat
     5041 ttacagcagc tgacgggtac ttaggaatcg ctattatctg gggagcgatg gcgctgtttt
     5101 ggtttgtcgg tgttcatgga ccgtcgatcg tggagccggc aattgcggca attatctatg
     5161 ccaatgtgga aaccaatctg caattgttca attccggtga acaagcttct aaagtactga
     5221 cggtgggttt agggaatttt gtaggaacta tgggaggaac tggggcaaca ttggttgttc
     5281 cttttatctt cctactcttt gctaagtcta agcagttgaa ggcagttggg aaagcttcct
     5341 ttattccagt aagtttcgct gtcaacgaac cattgctatt tgcggcaccc atgattttga
     5401 acccttattt ttttattcca tttttgttaa cacctgttgt aaacgtttgc ctgttcaagt
     5461 tttttgtcga tgtattgggc atgaacagct ttatgtacgt tttgccttgg gcaactccag
     5521 caccgatcgg tttggttcta ggaacgggca tgggtatctt agcttttgtt ctagcagcgg
     5581 ttctaatcgt cgttgacttc ctgatctatc taccattttg caaagcatac gatgctgtct
     5641 tagtagagaa agaagcacaa aaagcagagg tgatcgcaac cgaggaaggt gcttctgccg
     5701 atgtacgtga ggaagtacct gtggcagcga gtcaagttat cagcgatgca aataaagaaa
     5761 tcaatgtgtt ggtactttgt gctggcgcag ggacaagtgc gatgttagct aatgctttaa
     5821 aaaaaggtgc atctgaatcg aaagcaccga tcagtgcaat ggcgggtgct tatggttctc
     5881 attacgaaat gctgcaggat tttgatatgg tggtcttagc accgcaagtc aattcttact
     5941 atgatgatat caaagaagac accgatcgct taggaatcaa gctggttgcg acaaaaggag
     6001 cagaatatat cggattaacg agagatcctg aaaaagctgt tcagtttatt ctgaaccaat
     6061 ttcaataaag gaggagccat aatgaaaatg ggaatttggt tgcgtaaagc tgttttattt
     6121 gcagctgtgt taggggcttt gggggctgtt tcaaaaactg ttgaagcatc tgccagcgaa
     6181 tttattcgcg gagcagatat ttcgatcctg tcagatatgg aaaaatctgg tgcaaaatat
     6241 tatgaagacg atatttcaaa agatgcctta gagattttaa aaaataatgg tgtcaactat
     6301 gttcgactgc gtttgtggca ggatccttat aatagtaatg gagaaagtta tggagcaggg
     6361 acaaatgatc tggaaacgac gattgacctt gcaaaacgag caaaaaatct gggacttaag
     6421 gtgctgctgg attttcacta cagtgatttt tgggttgatc ctgggaaaca gaatcttcct
     6481 aaagcatggc aaggtctgac ctttgaagaa atgaatacgg cattgtacga ctatacaaag
     6541 aacgttctgt cggaaatgaa gcagctggac gtttatcctg atatggttca gatcggcaat
     6601 gaactgaata gcggaatgct ctggccttat ggcaagtctt ggggagaagg cgggggagaa
     6661 tttgatcgat tggctgcttt tttgaagtct ggtatccaag ctgttcgaga cactcaacct
     6721 aaaacaactc cagtaatgct ccacttagct gatggtggcg atacaggagc atttacttgg
     6781 tggtttgatg aaatcacgag tcgaggtgtg tcatttgatt taattggagt gtcgtattat
     6841 ccgtattggc acggttcaat gaatgaactt caaaccaata tgaatgcgat cagccaacgg
     6901 tatggtaaag aagtgatcgt agttgaaaca gcttatgggc atacaacagc gaatgccgat
     6961 actatgccaa atgcttttgg tgaagccgaa gcagctgcgg ggggatatga accaacaccg
     7021 acaggacaag cagagtattt gctggatctt gccgatcgga ttcaggcagt accgaataat
     7081 cgcggggcgg gcttcttcta ttgggaacct ctttggtaca acggaaatgt gagttgggca
     7141 acacaagcag gtatgaatta tctcagtgtt caatccacta tgggaaacga atgggacaac
     7201 caagcgatgt ttgattttgc tggaaacgcc ttgccaagct tgcgtgcgtt taaacaagca
     7261 ggggcgcaaa taaatctagt taaaaatgca agtttcgaaa gcgatggacc aacgaccagt
     7321 ccttccaatt ggcaaacctg gtttcaacaa ggaaccgcta agggtacgat caaaacggaa
     7381 gcgggcactt tttttgggga ctacaaacta actttttggc aggatcaagc atacgaggca
     7441 tcagcttatc aaaatgttag tggcataagc aaaggaactt acacattgag tgcctatgta
     7501 atgtctgggg gagagtttga tactaatcag ttatatatca aaaattatgg tggagcggaa
     7561 ctaaatactg cggttgttaa aacaagtgaa tggacaaaaa ttacaatcaa agacattcca
     7621 attacaacag gtgtttgtga aattggggtt tatagcaagg cttcaggaaa tgcttggacg
     7681 aacgttgatt ttgtttcatt ggtgaaacaa tagactgcgg atcgtttcga ttcaagatcg
     7741 gaggttccct tcagccacat cgttagcgta gaaggcagga atcgaacctg cacctcgcct
     7801 ttgccaaaaa taaccagaaa cgatttccag tcatttttac caaaacgagt aaagcaccag
     7861 acttcatcta cactttgtat tatgctaaag agagttcaac gatttcgtaa gaagcaatga
     7921 aaaaattgca aaaagttgtg cagataacat aaagtttgtt taagaaaggg taaccttaga
     7981 attccgtcgt gaatatgatt tgagcactta gatggataga aaaaagttct cggaaaacag
     8041 ttcgtttttc gagaacttct ttttatgata agaatgaaac aaattaactt atattatagt
     8101 ctcttttggt agggaaccat cttattttta atgaaaatca agcattttag ctattgaaga
     8161 atgttgattt atagggattc cccctccata ttatgttggc tctttattcc cactccactg
     8221 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     8281 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     8341 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     8401 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     8461 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     8521 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     8581 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     8641 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     8701 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     8761 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     8821 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     8881 cacctttcaa cttgtctaag aagcgatctc ttgcattgac tttgatgatg ttcaagccga
     8941 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     9001 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     9061 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     9121 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     9181 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     9241 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     9301 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     9361 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     9421 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     9481 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     9541 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     9601 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     9661 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     9721 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     9781 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     9841 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     9901 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     9961 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
    10021 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
    10081 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
    10141 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
    10201 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
    10261 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
    10321 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
    10381 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
    10441 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
    10501 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
    10561 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
    10621 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
    10681 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
    10741 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
    10801 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
    10861 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
    10921 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
    10981 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
    11041 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
    11101 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
    11161 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
    11221 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
    11281 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
    11341 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
    11401 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
    11461 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
    11521 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
    11581 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
    11641 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
    11701 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
    11761 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
    11821 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
    11881 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
    11941 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
    12001 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
    12061 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
    12121 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
    12181 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
    12241 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
    12301 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
    12361 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    12421 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    12481 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    12541 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    12601 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    12661 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    12721 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    12781 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    12841 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    12901 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    12961 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    13021 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    13081 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    13141 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    13201 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    13261 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    13321 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    13381 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    13441 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    13501 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    13561 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    13621 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    13681 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    13741 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    13801 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    13861 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    13921 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    13981 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    14041 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    14101 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    14161 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    14221 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    14281 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    14341 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    14401 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    14461 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    14521 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    14581 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    14641 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    14701 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    14761 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    14821 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    14881 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    14941 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    15001 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    15061 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    15121 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    15181 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    15241 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    15301 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    15361 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    15421 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    15481 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    15541 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    15601 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    15661 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    15721 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    15781 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    15841 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    15901 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    15961 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    16021 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    16081 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    16141 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    16201 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    16261 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    16321 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    16381 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    16441 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    16501 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    16561 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    16621 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    16681 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    16741 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    16801 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    16861 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    16921 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    16981 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    17041 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    17101 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    17161 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    17221 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    17281 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    17341 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    17401 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    17461 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    17521 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    17581 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    17641 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    17701 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    17761 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    17821 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    17881 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    17941 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    18001 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    18061 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    18121 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    18181 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    18241 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    18301 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    18361 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    18421 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    18481 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    18541 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    18601 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    18661 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    18721 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    18781 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    18841 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    18901 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    18961 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    19021 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    19081 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    19141 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    19201 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    19261 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    19321 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    19381 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    19441 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    19501 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    19561 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    19621 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    19681 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    19741 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    19801 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    19861 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    19921 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    19981 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    20041 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    20101 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    20161 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    20221 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    20281 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    20341 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    20401 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    20461 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    20521 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    20581 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    20641 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    20701 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    20761 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    20821 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    20881 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    20941 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    21001 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    21061 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    21121 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    21181 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    21241 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    21301 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    21361 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    21421 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    21481 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    21541 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    21601 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    21661 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    21721 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    21781 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    21841 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    21901 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    21961 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    22021 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    22081 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    22141 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    22201 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    22261 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    22321 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    22381 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    22441 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    22501 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    22561 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    22621 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    22681 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    22741 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    22801 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    22861 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    22921 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    22981 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    23041 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    23101 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    23161 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    23221 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    23281 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    23341 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    23401 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
    23461 cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt
    23521 tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc cttttttata gtgtttttta
    23581 taggtagtat tttttagcaa ctgttaagtc atcatttaat tcataaacaa gtggttgacc
    23641 tgttggaatt tcaagatcca tgatgtcttc gtcagagata ccttcgatat gtttagctag
    23701 ggcgcgtaat gagttaccat gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg
    23761 agcaatttca tcttgccaga atggcaaagc gcgttctaat gtcactttca agttttctcc
    23821 accaggaata tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg cagaaccttc
    23881 gtcagtttct tccattaatg gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg
    23941 ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg
    24001 acgttcgttt aagcgccatg atttgatttg tggtacccaa agttgatctg aatattctaa
    24061 gatcaagtta catgttttga ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc
    24121 gattccagct tctttgattt tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc
    24181 tagatttaca tcagcccaac cagtaaattg gttcaatgca ttccattcac taagaccgtg
    24241 acgagaaaaa actaattttg gcatgtttag ctctcctttt aagataggtt tatatttctg
    24301 aaattttcag ttctgtttta ttgtacaatt ttttggaaga gatttccatc gaaaaattag
    24361 ataagattct atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact
    24421 gttcgttttt aggtattaga tgcataattg ctcgatttct ttttcaggta aattgatata
    24481 tttataacat gttccaactg aaacaccgca ttttgaagaa ataaattgga tcgtttcttt
    24541 tttttcataa tagagacgtc ggattttttt gatcgttttt gcatcgattt ttggtcttcc
    24601 gccgattttt ccttttttac gagcttcatc taatccaact aatgtacgtt ctttgatcaa
    24661 tgcgcactcc atgtttgcta ggttttccat taattggaaa tagatttttc ccatttcgcg
    24721 cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg cgatctttga ataattgcgt
    24781 gagttctgtt aattgccgcg tagatttgcc taatcgatga agttggcaaa tgactagtgc
    24841 atcgccttgt tgcatgtcag cgatcactga ctccaaatga acagcagtag aatcattttg
    24901 ttccacttca tatgtctcat ggaaaatttc ttgacaacca aagtcagata attgatcaac
    24961 ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca taaccaataa ttttcatggt
    25021 gattcctttc tagcagtgat tttctaactg gatataacag taaaaatgta gcataaagta
    25081 cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt tttttattgg atgatcaagc
    25141 agtttttctt tatttacgac ggattgcgtc aagaatctca acgccttttt ctgagccgac
    25201 aactacttta gaaaccatgt ttaggaaaag cccatgttct actaccccaa caagttcatc
    25261 taaataggta gctaagcttt ctggctgttc gatgacttct agatgcagat caatgatgta
    25321 atgaccacca tctgtggtta agacttctcc gttttcgtct gtacgcaaga cgggttggaa
    25381 tcttttttct tcaaaaatat gaagtagctg ttgggagcca taagggatca cttcaactgg
    25441 gagtgggaac ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc
    25501 ttttgagtac gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc
    25561 ttgaaaatca gcactgattt catcagcacc gtctattgtc aaatcgacta cggggacttc
    25621 atcaatactc tttaacggta ttcctaattt ttccgcttgt tcttttgtcg catttgaagt
    25681 agtaactcct gtgatacgta aaccttcttc acgcatacgg cgaccaatct cttctaccat
    25741 ataataggcg gttgaaccgg tcccgagacc tacgatcatc ccgtccttta cgaatttcgc
    25801 ggcttcaatg ccagctaatt gttttaaatt catctttgcg tccacctttt tttagtttga
    25861 gcatcgtgct ttcctcacta ttttagcgtg atttacggta aaaagatacc attctttttt
    25921 attccaataa agctttttca aaaaaaattg catctcttgt tgacatgttt tttcattcgt
    25981 gatattctaa caaaggtgct ttatctacag gtttctgtga agaaacgtgc tgactgttta
    26041 tcaaagcata tttgataagg atgcagacat tgcatttatt ttttatcaca aaaaaagaac
    26101 cacctggatg tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca
    26161 attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct
    26221 ttgaacaaag gatataacag ctttaagaaa actcaaacta acgtaaactc acctcaaaaa
    26281 cgtggggtag caactcgtgt gggtacaatg acacctaaaa aaccgaactc agctttacgt
    26341 aaatatgcac gtgttcgttt gtcaaactta attgaagtta ctgcttatat cccagggatt
    26401 ggtcataact tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt aaaagactta
    26461 ccaggggtac gttaccatat cgttcgcggt gctttagata ctgctggtgt taacgaccgt
    26521 aaacaaagcc gttctaaata cggaactaaa aaacctaaag cttaatttta attattaaac
    26581 aaatgaaact tgcttctact atatagaagt aattactatg aacaatattt cggaaggagg
    26641 agttacggat gccacgtaaa ggtcctgttg caaaacgtga tgttttacca gatcctattt
    26701 ataactcaaa attagtaact cgcttgatca accgtgtaat ggttgacgga aaacgcggga
    26761 ttgcagctaa cattatctat aatgcctttg acttgatcaa agaatctaca ggtaacgatc
    26821 cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac
    26881 gtcgtgttgg gggttcaaac taccaagtac cagttgaagt tcgccctgaa cgccgcgtta
    26941 ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac acaatggaag
    27001 aacgcttagc aaaagaaatc atggatgctg caaacaacac aggtgcttct gttaaaaaac
    27061 gtgaagacac tcataaaatg gctgatgcca accgtgcgtt tgctcactat cgctggtaag
    27121 attctctctg tttggtcggc ttttataagt cgacctcaga tagtgtcctt atttttagca
    27181 actagatagt aaagagagga gaaacaagga gaatgaccag agaattttct ttacaaaaca
    27241 ctcgtaatat cggtatcatg gcccacatcg atgctggtaa aacgactgct actgaacgta
    27301 tcttgtacta tactggtaaa atccataaaa ttggtgaaac tcatgaagga gcttcacaaa
    27361 tggactggat ggaacaagaa caagaacgtg gtattacgat cacttctgct gctactacag
    27421 cacaatggaa gggtcaccga atcaacatca tcgacacacc aggacacgta gacttcacag
    27481 ttgaagttga acgttcattg cgtgtacttg atggtgctgt aactgttctt gatgcacaat
    27541 caggtgttga acctcaaact gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc
    27601 gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga cttcttatat tcagtatcaa
    27661 ctttacatga ccgtttgcaa gcaaacgctc acccaatcca attaccaatt ggtgcggaag
    27721 acgacttcac aggaattatc gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc
    27781 ttggaacaga gatcgaagaa acagaaattc ctgaagaata ccgcgaatta gctgaagaat
    27841 ggcgcgaaaa attggttgaa gctgtagctg aaactgatga agaacttact ttgaaatact
    27901 tagaaggtga agaaatcact gaagctgagt taaaagaagg tatccgccgc gcaacagtta
    27961 acgtagaatt ctacccagta ctatgtggtt cagcatttaa gaacaaaggg gttcaattac
    28021 tattggacgc tgttcttgat tacttgccat caccgttaga tatcccagct atcaaaggga
    28081 ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga cgacagcgct cctttctcag
    28141 ctttagcatt taaagttatg actgacccat tcgtaggtcg tttaactttc ttccgtgtgt
    28201 actctggtgt tttacaatca ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac
    28261 gtgttggtcg tatcctacag atgcatgcca actctcgttc agaaatctct gaagtttacg
    28321 ctggagatat cgctgctgct gttggtttga aagacacaac tactggggac acactatgtg
    28381 atgaaaagag tctagttatt cttgaatcca tggaattccc agaaccagtt atccaagtcg
    28441 ctattgaacc taaatcaaaa gctgaccaag ataaaatggg tgtggcttta caaaaactat
    28501 ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg
    28561 ctggtatggg tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag
    28621 ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga aactttccgt gctggcactc
    28681 aagccgaagg taagtttgta cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga
    28741 ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg
    28801 gggttgttcc tcgtgaatac attccagctg ttgaaactgg tttgaaagat gctatggaaa
    28861 atggtgtctt agctggctat ccattagtag atatcaaagc taaactttac gatggttcat
    28921 accatgatgt cgattcaaat gaaactgcct tccgtgttgc tgcttctatg gcattacgtg
    28981 cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat catggctgta gaagttgtta
    29041 tccctgaaga ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg
    29101 aaggtatgga agctcgcgct ggcggccaac aagttgttcg tgcgatggtt ccacttgctg
    29161 aaatgtttgg ttatgctaca actcttcgtt ctgctactca aggtcgtggt acatttacaa
    29221 tgacatttga ccactacgaa gatgtaccaa aatcagtaca agaagaaatc attaagaaaa
    29281 acggcggaaa agctgaataa tttcttgaaa ccggatagtc ctgcaaagag attatccaaa
    29341 gagactatat ttttaatata gtttcgtgta aaataattat cgatgatatg ggcatgtggt
    29401 gtaccttgtg tacatcatat acctatcaga caaataaaaa atatattgta tttttaggag
    29461 gacattttaa aatggcaaaa gaaaaatttg accgttctaa accacacgtt aacattggta
    29521 ctatcggaca cgttgaccat ggtaaaacta cattaacagc tgcaatcaca actgtactat
    29581 ctaagaaaaa cggcggccaa gctatggctt acgatcaaat cgatggtgct ccagaagaac
    29641 gcgaacgcgg aatcacaatc tcaacagctc acgttgagta tgaaacagat acacgccact
    29701 acgctcacgt tgactgccca ggacacgcgg attacgttaa aaacatgatc actggtgctg
    29761 ctcaaatgga cggagctatc ttggtagttt ctgctgctga cggcccaatg cctcaaactc
    29821 gtgaacacat cctattgtct cgtcaagttg gtgttcctta catcgttgta ttcttgaaca
    29881 aagtagacat ggttgatgac gaagaattac tagaattagt tgaaatggaa gttcgtgacc
    29941 tattaacaga atacgaattc cctggtgacg atgttcctgt agttgctgga tcagctttga
    30001 aagctctaga aggcgacgct tcatacgaag aaaaaattct tgaattaatg gctgcagttg
    30061 acgaatacat cccaactcca gaacgtgaca acgacaaacc attcatgatg ccagttgaag
    30121 acgtgttctc aattactgga cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag
    30181 ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga agaaacttca aaaacaacag
    30241 ttactggtgt tgaaatgttc cgtaaattgt tagactacgc tgaagctgga gacaacattg
    30301 gtgctttact acgtggtgtt gcacgtgaag acatccaacg tggacaagtt ttagctaaac
    30361 caggtacaat cacacctcat acaaaattct ctgcagaagt atacgtgttg acaaaagaag
    30421 aaggtggacg tcatactcca ttcttcacta actaccgtcc acaattctac ttccgtacaa
    30481 ctgacgtaac aggtgttgtt gaattaccag aaggaactga aatggttatg cctggcgaca
    30541 acgtaacaat ggaagttgaa ttaatccacc caatcgctat cgaaaacggt actaaattct
    30601 caatccgtga aggcggacgt acagttggtg ccggtgtcgt tacagaaatc aaagcttaat
    30661 tcacttaagt tttaaagtaa agcaacattt attcggacgt aatcgctcgg aacgaaggtc
    30721 ttaggatctt tgttccgagc ttttttattt attgcaggat caggaaaaaa gacaggagta
    30781 ttataaatag aagaaacacg aaataaaaga gtcagtattt tctaaatgaa actttatgaa
    30841 aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac
    30901 aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag
    30961 aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc
    31021 gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa
    31081 attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc
    31141 gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa
    31201 cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc
    31261 gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat
    31321 gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa
    31381 aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg
    31441 acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca
    31501 aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt
    31561 taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca
    31621 aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa
    31681 gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt
    31741 actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca
    31801 atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac
    31861 cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa
    31921 aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt
    31981 cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa ataattgtag
    32041 gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa
    32101 aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt
    32161 tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac
    32221 cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca
    32281 cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca
    32341 ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta
    32401 ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca
    32461 ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg
    32521 gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt
    32581 tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta
    32641 gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg
    32701 taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca
    32761 cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt
    32821 ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg
    32881 gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt
    32941 caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt
    33001 aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat
    33061 gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc
    33121 attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg
    33181 cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc
    33241 aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta
    33301 tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact
    33361 tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc
    33421 agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg
    33481 ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt
    33541 gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg
    33601 taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa
    33661 acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga
    33721 aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg
    33781 ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa
    33841 taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag
    33901 tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg
    33961 ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt
    34021 tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga
    34081 agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc
    34141 tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac
    34201 aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt
    34261 tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca
    34321 caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag
    34381 aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag
    34441 gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac
    34501 gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa
    34561 aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat
    34621 gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca
    34681 actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta
    34741 aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc
    34801 gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg
    34861 gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta
    34921 gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct
    34981 ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt
    35041 tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac
    35101 gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt
    35161 ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac
    35221 ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg
    35281 gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa
    35341 accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag
    35401 tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg
    35461 gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt
    35521 ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca
    35581 aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag
    35641 gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa
    35701 gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt
    35761 attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag
    35821 taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat
    35881 caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag
    35941 aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat
    36001 gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca
    36061 gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac
    36121 gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa
    36181 gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa
    36241 gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt
    36301 aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt
    36361 cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct
    36421 gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca
    36481 cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa
    36541 tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa
    36601 gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg
    36661 aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct
    36721 caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg
    36781 atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa
    36841 aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga
    36901 gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg
    36961 atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc
    37021 aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa
    37081 ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga
    37141 ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg
    37201 tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt
    37261 gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg
    37321 atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt
    37381 gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca
    37441 ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac
    37501 aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca
    37561 cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa
    37621 ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag
    37681 gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg
    37741 caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg
    37801 ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac
    37861 aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact
    37921 taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag
    37981 tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag
    38041 aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa
    38101 tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa
    38161 ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg
    38221 actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact
    38281 cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac
    38341 aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg
    38401 taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta
    38461 gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt
    38521 aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct
    38581 ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa
    38641 ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg
    38701 catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt
    38761 ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt
    38821 gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa
    38881 tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta
    38941 cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa
    39001 agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga
    39061 acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca
    39121 tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa
    39181 ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt
    39241 gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct
    39301 ttagttgttg tcggtgacaa aaacggacac gtaggatttg gtacaggtaa agctcaagaa
    39361 gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct
    39421 atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta
    39481 atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta
    39541 gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac
    39601 gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa
    39661 cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat
    39721 taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta
    39781 aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta
    39841 aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa
    39901 tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct
    39961 gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa
    40021 actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga
    40081 tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg
    40141 aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga agacggagct
    40201 gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc
    40261 aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct
    40321 aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta
    40381 gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat
    40441 ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt
    40501 cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg
    40561 cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg
    40621 tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg
    40681 aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta
    40741 tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata
    40801 atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg
    40861 ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca
    40921 tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact
    40981 ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag
    41041 tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc
    41101 cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag
    41161 taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg
    41221 ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg
    41281 agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct
    41341 tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa
    41401 agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa
    41461 gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc
    41521 caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct
    41581 tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga
    41641 aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc ctcaacacac
    41701 gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc
    41761 gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca
    41821 acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag
    41881 tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag
    41941 cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta
    42001 gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc
    42061 atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc
    42121 agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt
    42181 gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa
    42241 aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa
    42301 ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa
    42361 aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg
    42421 tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg
    42481 atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat
    42541 gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg
    42601 tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca
    42661 agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga
    42721 caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt
    42781 taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta
    42841 aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc
    42901 ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc
    42961 gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct
    43021 atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg
    43081 ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat
    43141 taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa
    43201 tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga
    43261 aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat
    43321 tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg
    43381 tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat aacactatca
    43441 tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt
    43501 tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa
    43561 aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg
    43621 gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg
    43681 acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag
    43741 tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa
    43801 ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa
    43861 aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg
    43921 aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa
    43981 atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa
    44041 atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt
    44101 gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat
    44161 gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat
    44221 gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa
    44281 gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac
    44341 tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag
    44401 atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg
    44461 actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg
    44521 gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac
    44581 ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt
    44641 acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa
    44701 gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt
    44761 ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt
    44821 aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact
    44881 gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc
    44941 ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg
    45001 cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt
    45061 gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga
    45121 cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc
    45181 actttataga tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc
    45241 tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt
    45301 tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa
    45361 gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga
    45421 agaaggagta atcatggagc caattattga attagaaaag ataaattata aataccagcc
    45481 agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat
    45541 cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt
    45601 gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat
    45661 ttggacgatc cgacagatgg ttggaatggt ttttcaaaat ccagacaatc agtttgttgg
    45721 ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga
    45781 aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg
    45841 cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc
    45901 cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg
    45961 tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc
    46021 tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg
    46081 cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga
    46141 cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt
    46201 tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt
    46261 tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt
    46321 gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc
    46381 gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc
    46441 catattggag aacgagatat tcagccggat acggataata agaatttaaa gcccatccga
    46501 aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca
    46561 aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta
    46621 gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt
    46681 gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg
    46741 gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg
    46801 atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat
    46861 ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc
    46921 gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa
    46981 ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt
    47041 gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga
    47101 aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca
    47161 tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt
    47221 agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt
    47281 gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct
    47341 cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg
    47401 tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt
    47461 gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga
    47521 tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc
    47581 attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat
    47641 catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat
    47701 gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga
    47761 tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg
    47821 tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt
    47881 gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct
    47941 gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca
    48001 gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga
    48061 aaaatcatta ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca
    48121 aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc
    48181 gtactgttca agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca
    48241 cggtctttgg ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt
    48301 ttgattatcc agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac
    48361 cagaggatat tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt
    48421 tggtcaaaga aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc
    48481 gacgccatta tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc
    48541 ttcctgattt attagggact cacgatttca cctctttttg tgcatcagga agctcgatag
    48601 aggataaaat acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat
    48661 tgttgtttac atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa
    48721 cgcttttgaa aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga
    48781 aaaaagatcg taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag
    48841 tgaaatatga ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt
    48901 acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct
    48961 ggaacaccgt ttaaggaaga ggtcgtgaaa tcagcgtttt gacctgagta ttagagaaac
    49021 aaacgggaaa atagctcctc atattttgcc gtttgttgag ctaataccga aggtcagctg
    49081 attgaacacc gtttaagaaa gaggtcgtga gcctgtcatt taactctgag tcacaaagag
    49141 caatttcaaa aaacagcttc tcatctttga tgaatatcgt gacttgtgga cgaggcgttg
    49201 ataagtgaat catctttttg ttacctattt tttctgtaac caaaaaacga tggaacgttg
    49261 ttttgattgc ccttggtgcg gacagtctga acaggttcca tcgtttcttt attttctacg
    49321 tcttcttctg tgtgtgtttc gtggttgttc ttctattgct ggatcaatcg tgccataaaa
    49381 ggggcgattg cggtcaaggt accagacata aagaataaac aatgcaatac ataggacaaa
    49441 aaggattgca cctatcgtaa atccttcagg caagtaactg aaagtgacag tatgtttacc
    49501 tgctggaact tttatcatca agaatgcgtc tttgaaattg ctgaccttga ctggtttccc
    49561 atcaatttta gctttccatc ctttgtcata aggaatagtt gtcaccaaca tggaatcttc
    49621 agcggtattt acagtcccag aagcttttct gccagtggct tccatttcga cacctttttc
    49681 tttgatctga ttcattgcgg cttgataggc tacaacatct agaccaacga ctttaggctc
    49741 catgaaactt atttctttcg tcccataaaa acttgcagta aatgtcactg ttgtctcttg
    49801 aggataatag ccaagatcat agtattgacc agaaatgttg atttgcgttt ttcgcgatgt
    49861 accattgaca gtgatcgtcg cactagaact ttctaattgc gcaaaattgg ttgggaacaa
    49921 acttaagtaa gcttgtgtat ttgcaggcac atggacttcc caagtgagct cttttggctg
    49981 gttatctgct gtttctgagt aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa
    50041 ctgattcttc aaagtaggtg tatagaatgt aaaatactgt tcatttagac cagacaatgt
    50101 attgatcaag tttgtctgac tggtcaaatt atctgtcact ggctgctcaa catcattaac
    50161 ggacaacggt gcagtaaatg ctagtggcaa tgcattgaca ttctgataca gtgtatagtc
    50221 gccgctttga ccagcttggc ggaagccata tttactgaac gtgctttcgt ttttagaaat
    50281 gttatatttg attcctgtaa acccatccat cagcaaagta ttattgttat aacggatatt
    50341 caaatttgtc cctcttgaac ggaaacctaa tttatccagg taagaggaag aattacgatt
    50401 tcttatagaa gaaaacaaac tgataccact gtaaccatag ttgatcgaat cgtttgcaga
    50461 tactggatcc agattttcca agcggtaaaa cgagtcggaa tctttttttg tttcatttac
    50521 aagagctttc aagtcaggat aaggatcagt atataaactg cgggaagcat aattccaatc
    50581 atcaagaatg ccatggatca tactgttcgt attgaccagt gcttcagcag acatgatcaa
    50641 taataacaat atactcagat gattgaatga aagtttttta tgctgataaa atcccacagc
    50701 aaaagcataa atccctaaga aaaccactgt caatacaaag gaagtcatcg tcacataggt
    50761 gtagcttcct tcaggtttga tgccccatgc caccgcaaag agagcagcta cgaccaaaat
    50821 gattcctaac aacagcccac gatcgtcttg ttctatcttt tcccatcctt ttcctgcaag
    50881 caggagcact aagaaagaga acagataggc ataacggaat aaaaacatat tcggtgcatg
    50941 cataccatgc cagaacaaat tcaatggaac aagataaaaa cttgcgataa gtatggcgaa
    51001 aagactgccg tacaaaaatt tatttttcaa cggtactttc cgactggcaa aataaaacag
    51061 acagaggact aatggtagca gacctgcgta aacgaacgga atcgatccgt attttgtcgt
    51121 gtcataaaca ccaatcatat ttttcatgat tagatccaga taagaagtcg cttctgtttt
    51181 caacgtagtg atctgactca gtgcctcgcc atttgaccga agatccagca ctgctggcaa
    51241 tacaatgatc attgaagcgc cgcctgccag taaagaagtg ataccataag gaacaatcgt
    51301 ttttttatat tctgaccagt ttgtcaatag acgggcgata aaatataaga aagaaaatac
    51361 gccaatcata aaacccatat aaaaactgga taaaaacaag agtaaataac tgataaataa
    51421 aagtttcggc cgtttaagat ccataacacg atgaatcccc aaaatcacta atggaagata
    51481 aaaaaatgca tccagccaca tgatgatctc cgaatgtgca gtgataaatg agatcaatgc
    51541 gtaactgacg ctaagtgcaa catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa
    51601 ccaaaaagcc aatccggcac agccgacctt aagcaaagtc agaacataca gtgcatctgg
    51661 catcatctgg tttggaaaaa agaacaccag tggcgtgaag atcccgccta gatagtaaga
    51721 aattaaagat aaataattca atcctagaga agcattccat gtataaaaaa tactttgctt
    51781 tccaagaaga acatttcgga agctggcatg aaaattagaa aactgtgaaa aggcatcact
    51841 tgccaaaata cttctgctgc ttccaggata gattccgata cttagataaa caagtgctaa
    51901 aatcaaaaac ggaatcagga aactagctgc tgcgtagcgt ccgttttctc taaagaatga
    51961 ctttaaattt tttttcatat ttattttcca ccaaatttct atgaagttgt attcagttt
//
LOCUS       DAJSVD010000006        50390 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSVD010000006 DAJSVD010000000
VERSION     DAJSVD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.006"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..360
                     /locus_tag="ON498_000283"
     CDS             <1..360
                     /locus_tag="ON498_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCR3938586.1"
                     /translation="IYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNV
                     SKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTV
                     SNDKDGVSQAFERILTI"
     gene            641..1861
                     /locus_tag="ON498_000284"
     CDS             641..1861
                     /locus_tag="ON498_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR3938587.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1858..4578
                     /locus_tag="ON498_000285"
     CDS             1858..4578
                     /locus_tag="ON498_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3938588.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4562..5506
                     /locus_tag="ON498_000286"
     CDS             4562..5506
                     /locus_tag="ON498_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3938589.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5575..6585)
                     /locus_tag="ON498_000287"
     CDS             complement(5575..6585)
                     /locus_tag="ON498_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3938590.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6765..7109)
                     /locus_tag="ON498_000288"
     CDS             complement(6765..7109)
                     /locus_tag="ON498_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3938591.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7102..7536)
                     /locus_tag="ON498_000289"
     CDS             complement(7102..7536)
                     /locus_tag="ON498_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR3938592.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7674..8411
                     /locus_tag="ON498_000290"
     CDS             7674..8411
                     /locus_tag="ON498_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3938593.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8404..9615
                     /locus_tag="ON498_000291"
     CDS             8404..9615
                     /locus_tag="ON498_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938594.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9819..10601
                     /locus_tag="ON498_000292"
     CDS             9819..10601
                     /locus_tag="ON498_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR3938595.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10671..11333
                     /gene="trmB"
                     /locus_tag="ON498_000293"
     CDS             10671..11333
                     /gene="trmB"
                     /locus_tag="ON498_000293"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3938596.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11431..13185)
                     /gene="ade"
                     /locus_tag="ON498_000294"
     CDS             complement(11431..13185)
                     /gene="ade"
                     /locus_tag="ON498_000294"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3938597.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13317..14246
                     /gene="tadA"
                     /locus_tag="ON498_000295"
     CDS             13317..14246
                     /gene="tadA"
                     /locus_tag="ON498_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR3938598.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14203..15252
                     /locus_tag="ON498_000296"
     CDS             14203..15252
                     /locus_tag="ON498_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR3938599.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15249..15527
                     /locus_tag="ON498_000297"
     CDS             15249..15527
                     /locus_tag="ON498_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3938600.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15502..15972
                     /locus_tag="ON498_000298"
     CDS             15502..15972
                     /locus_tag="ON498_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR3938601.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15989..16291
                     /locus_tag="ON498_000299"
     CDS             15989..16291
                     /locus_tag="ON498_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938602.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16308..16679
                     /locus_tag="ON498_000300"
     CDS             16308..16679
                     /locus_tag="ON498_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR3938603.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16651..17010
                     /locus_tag="ON498_000301"
     CDS             16651..17010
                     /locus_tag="ON498_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938604.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17182..18189
                     /locus_tag="ON498_000302"
     CDS             17182..18189
                     /locus_tag="ON498_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3938605.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18214..19398
                     /locus_tag="ON498_000303"
     CDS             18214..19398
                     /locus_tag="ON498_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3938606.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19835..21418)
                     /locus_tag="ON498_000304"
     CDS             complement(19835..21418)
                     /locus_tag="ON498_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3938607.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21996..22139)
                     /locus_tag="ON498_000305"
     CDS             complement(21996..22139)
                     /locus_tag="ON498_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR3938608.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22361..22840)
                     /locus_tag="ON498_000306"
     CDS             complement(22361..22840)
                     /locus_tag="ON498_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3938609.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22913..23197)
                     /locus_tag="ON498_000307"
     CDS             complement(22913..23197)
                     /locus_tag="ON498_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938610.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23275..23469)
                     /gene="ssrS"
                     /locus_tag="ON498_000308"
     ncRNA           complement(23275..23469)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON498_000308"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23541..24827)
                     /locus_tag="ON498_000309"
     CDS             complement(23541..24827)
                     /locus_tag="ON498_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR3938611.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24965..25636
                     /locus_tag="ON498_000310"
     CDS             24965..25636
                     /locus_tag="ON498_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR3938612.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25602..26090
                     /locus_tag="ON498_000311"
     CDS             25602..26090
                     /locus_tag="ON498_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR3938613.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26103..27050
                     /gene="prmA"
                     /locus_tag="ON498_000312"
     CDS             26103..27050
                     /gene="prmA"
                     /locus_tag="ON498_000312"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3938614.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27051..27815
                     /locus_tag="ON498_000313"
     CDS             27051..27815
                     /locus_tag="ON498_000313"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3938615.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28048..30261
                     /locus_tag="ON498_000314"
     CDS             28048..30261
                     /locus_tag="ON498_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3938616.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30272..30718
                     /locus_tag="ON498_000315"
     CDS             30272..30718
                     /locus_tag="ON498_000315"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3938617.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30889..32148
                     /locus_tag="ON498_000316"
     CDS             30889..32148
                     /locus_tag="ON498_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR3938618.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32237..33184)
                     /locus_tag="ON498_000317"
     CDS             complement(32237..33184)
                     /locus_tag="ON498_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3938619.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33578..34870
                     /locus_tag="ON498_000318"
     CDS             33578..34870
                     /locus_tag="ON498_000318"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3938620.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34885..36654
                     /gene="aspS"
                     /locus_tag="ON498_000319"
     CDS             34885..36654
                     /gene="aspS"
                     /locus_tag="ON498_000319"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3938621.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36763..37638
                     /locus_tag="ON498_000320"
     CDS             36763..37638
                     /locus_tag="ON498_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3938622.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37904..39787
                     /locus_tag="ON498_000321"
     CDS             37904..39787
                     /locus_tag="ON498_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3938623.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39862..40146)
                     /locus_tag="ON498_000322"
     CDS             complement(39862..40146)
                     /locus_tag="ON498_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3938624.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40136..40870)
                     /locus_tag="ON498_000323"
     CDS             complement(40136..40870)
                     /locus_tag="ON498_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR3938625.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40999..41622
                     /locus_tag="ON498_000324"
     CDS             40999..41622
                     /locus_tag="ON498_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR3938626.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41814..42935
                     /locus_tag="ON498_000325"
     CDS             41814..42935
                     /locus_tag="ON498_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR3938627.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42942..46070
                     /locus_tag="ON498_000326"
     CDS             42942..46070
                     /locus_tag="ON498_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR3938628.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46089..49283
                     /locus_tag="ON498_000327"
     CDS             46089..49283
                     /locus_tag="ON498_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR3938629.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49429..50136)
                     /locus_tag="ON498_000328"
     CDS             complement(49429..50136)
                     /locus_tag="ON498_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3938630.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16446 a         8855 c        10502 g        14587 t
ORIGIN      
        1 atttacaaat tttttatttt tattcctaat cgtcaattga aagctaatat atttaaccat
       61 ttgaaaaaga tgctaaatgt gtctgttact gaatcggcac ctgtaaatat tgaaatcgta
      121 cctcaaaatg tatctaaagg tgtggtgttt gatcgtttgg aatcaatcta caatttaaag
      181 aaacctttga gaatcgcgat cggagatagt ttaaatgact tatctatgtt tgaaagtgca
      241 gatattagtt ttgcaatggg aaatagtcat caagaaatca tgaaaaaagc tgattatgtc
      301 actgtttcaa atgataaaga cggagtatct caagcatttg aaagaattct taccatataa
      361 actaattaag gtagtataga acatagaatt ctaaatacgg aaaaatggag cataaattcc
      421 agtacaaata gtacttcttc agtgtcaaac gaagcgaata taacatagat atttaggatt
      481 gtgacaaaaa tcatgtcaca atcttttttt cgaataaact tattttttga agtcggccat
      541 ttatcgtaat ttctttgcat aatacgaaca aattttcggt tattgatgaa aaaaaacatt
      601 gaaaatggta tgataaaaga aaaaaaggag gaaattttta atggtgaaat ttatccatgc
      661 ggcagattta catatggatc ggtcatttga aggactgaca actcttgata aaactgtaca
      721 ggaaaaacta ttgaagacga atctgaccgt tttatcaaat atcattgaac aggcaataat
      781 aaataacgtt gattttgttt tacttgcagg agataatttt catcaaaatc gtccttcatt
      841 aaaaatacag aaacattttt cagaacagat gaaacggtta gaaaagaacc atatacctgt
      901 atttataata ttcggaaatc atgatttcta tcaaaaagag cggtattggt tctcctttcc
      961 taaaaatgtc catttattta catccgaaac ggtagagaca aaaaaaataa cgataaaaag
     1021 tggagaaact gtctctctca gtggatttag ttatcgtcaa ccgtggattc aaaaagacaa
     1081 agtgatggaa tttccttcta gagaactcac agattaccat attgggttgt accacgggga
     1141 acctggcgtc tctcaaaaag gaaactatgc tcctttccaa ccctcaaaga tgcaagaaaa
     1201 gggatacgat tattgggctt tagggcatat ccatgtgcca acggtcttaa atgaaaagca
     1261 aacaattgtt tatccaggtg cgcctcaagg tcatactaaa aaggaacaag cttctacatc
     1321 tattctacta gttgaattaa gtaaaggaag ttgccaaata catccgatta aagtagcaga
     1381 ggtctattgg aagacaaagg aaatatcatt gaggaaagca agaacgacaa aagaggtcat
     1441 tgttcacatt cgtcagcagc tttcaaaagt agaggatgga tttagtttaa ttgagataaa
     1501 attaaaagat tatgaccatc tcaatacaga tgttttggaa cggatacaat caggagagct
     1561 attagactat ttgttagagg aattctcaga taggataaat gatttcttta tttggcgtat
     1621 ttctctcata gagaatactt tttatacgaa gaccccactt gctgcttctg ctaaattgat
     1681 ggaacagtta tttcaatact atcagacacc tcaagatttc caacaaattt taaatgaagt
     1741 atatagtcat caagaagcgg ctagaatatt gagcgaactg ccggaatatc aagaagaaac
     1801 acttgaaaaa gccaagcagc tgcttaatca agatttttta tttgaggagg atcaggaatg
     1861 aagattttaa ggatcgagat tgctgctttt ggaaaatggc gtcaaaaaag ttttgacttt
     1921 tattcaggca atcagctgat ctatggggga aacgaagcag gaaaatcaac gatttatcaa
     1981 tttattcaag caatattatt tggtttccct tcaaaaggcc gtaaaaaaaa ggattataca
     2041 ccaaaagacg gctcagccta tggcggcaaa atatggctta aacatcctgt atatggtgaa
     2101 tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat cgaaggtgtg gctgggggat
     2161 caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac ctttgacgaa agagttattc
     2221 cagcaggtat ttactttcca gcaagagcag ttgatggaac tagatcattt gagagaaaaa
     2281 gagctacatg atgcgctgat ttctttaggc atcacgggca gttctcaagt attccaaaag
     2341 cggcaagaat attatcagaa agcacaacaa ttattcaaga aaaaaggcca aaaattacta
     2401 atcaatcaaa aattaaatga atggaaagag ctgcaagaaa aaattcatca aaaacaagaa
     2461 caggaagctt attttggcaa gttgatccaa caagaacagg aatacgagtc gcaacagcag
     2521 cagttattgg cacaattaaa agaggcgaac aaacaatttt cattagccag acagcaagaa
     2581 cttaattttt ctttatatga agaatggcaa tctctcaaaa aaaacagcac tacaggtctt
     2641 cctgatgagc aacttctttt agatctggag tctttctcca aacggtatca gcaaacaaat
     2701 gagcgtttgg aagaattaga agcaacactg gaaaagcata gcgggatgga tcagcagtca
     2761 gccagatatt atttttattt agagcaggag caggaactaa aagatctcca gcaacttcaa
     2821 tttacagcgg aacaattaaa cgaagaatgg caaagattac aaattgagac tagagaaaca
     2881 aattcagaat tgaagatttt ggaacaaagg tggcattggc agaaaaatcc gttaccggaa
     2941 gaatttttat atgaagaaga atggaagcat cttctggctg aagaaagaag gattgctgat
     3001 ctagaaagcc agatttctgt gcgtctccaa atagctaaag aacagcaaga gatgattgaa
     3061 gcagaagtga atgacttgga agagacctat ccagcattat tacaaaatca gtcagagata
     3121 gcaccagtct cttcatctaa accgtatatg ctgatggctg gcggagcaat aatcattgca
     3181 ggtatatttg taccaatgcc cttgaaagtg atccttttac taattggtgc aggcatgatt
     3241 ggctatttct tctacaaaag gcaaccaaaa attaaaacta aaaatgattt ctctgagata
     3301 aaaggaatgt ggcaagaaaa actaggacaa cttgatctag ccttagctca attagctgaa
     3361 gcagaagaaa aacaaaagag aactggagaa gaaaaacaga ttcttcagca agaagtatat
     3421 cgttttgcgg atcatcggca cttaggaaag atggatacgc tcaaactcat gaaagaatat
     3481 ggacaggaag tcatagatta tcagaaatta tatggcgtat ttgagcggaa acataaaaga
     3541 gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa aatttgagtt tgttaaagaa
     3601 tggttaccga tacaagataa gacaatttca gaaaaaatgg tattgctttc ccgttttata
     3661 caagagatgg aacagatgaa atttgccaga agctatcagc aaaatacttt attgaagcaa
     3721 gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac aagaatacag cgaacaattg
     3781 actcaagcag gcttatctta tccttcggaa gtacctgtct ttttacaaaa agccaaacaa
     3841 gccgaacagc agatgaaaag atacaatgaa ttatcggaaa tgcttactcc actatttcct
     3901 gaagagctga caaaagagaa attatctgta agactgcgcc aactgaaaga aaagcaatcc
     3961 caactccagc aagaagaaaa agaagcaagt gaaaatcggc aacgactcca acttcaagta
     4021 gaagagttgc aaagagacgg cacattggac gaactttatc aacaagctag ccggcaaaaa
     4081 gcaaaattac aggaattact ggaggaatgg ggagcacttg aaatcgccgg gacattctta
     4141 ggtgatcttt caacagagat gagtgaaaga caattacccc agttgttaag agcagcaggt
     4201 gattatttag gtattttaac aaaaggaaat tatcagaaga tattactgaa aaatgataca
     4261 ctgactttaa cagacggata tacagaattt cccatctatg agttatctac aggtacgaaa
     4321 gatcagctga ttatggcgat gagatttgct tacctctcat tagaagctaa ccgttcgata
     4381 tgcccgatca tcattgatga tggctggctt cattatgatc atcagcgaaa gtatcaatta
     4441 gctgaacttt tagctaaatt tggagaaaag tatcaaataa tttgtttttc ttctgataaa
     4501 gaaatggtaa gctattacca tgagatgaac caaatcgtaa aacaattaga aggagcaaat
     4561 aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt gaacaatttg tcctgatcaa
     4621 aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt attgctttta ctttccaaga
     4681 tacttcaggt actatcgatg gtaaatactg ggatgcttcg gaggaggaaa tcaataaatt
     4741 tacagccggc acagtcgttt ttctaaacgg taaaagagag gtttatcaag gaaaccctca
     4801 agtaaagatt cttcatatgc gcttaacaag ggtagatgag ccaggagagc ctagtttata
     4861 catggaaaga gcccctttga agaaagaaga aatggaagaa gaaatcaatc aattgttgtt
     4921 tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat ctacttaata aatatcaaaa
     4981 acaatttttt gagtttccgg cagcaaaaag aaaccatcat gcatttgcca gtggattagc
     5041 ttatcatact ttgacaatgc ttcacttagg gaaatcaatc gccgatgaat atcctgagct
     5101 gaatcgagca ttgttgtatg ctggtatcat tttgcatgat ttaggaaagg tgattgaact
     5161 ttccggtcct atgtcgacag aatatacgct tgcaggaaat ttgatcggtc atctggtatt
     5221 gattgacgaa gaaataacta aagcctgtct tgcgttgaaa atcagcgaag ctgacgaaga
     5281 tgttcttgta ttacgtcata tggttttagc acaccatggc ttattagaat atggctcacc
     5341 tgtacgtcca agaataatgg aagcagaaat attacatcaa atcgacaata tcgatgcttc
     5401 gatgcagatg atgttgactt cgattcgtca aacagaacca ggaaaataca ccgatcggat
     5461 tttcggtatg gacaaccgaa gtttctatgt accaaaagat ttatagcaaa aatttatttt
     5521 tttcattcaa aacaaaaaaa gcgagacata agtcttcgct ttttttgttt tcttttattt
     5581 tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg attttgtgtc
     5641 agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg aagttgttgt
     5701 gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg catcttttaa
     5761 ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt cagtagcgat
     5821 atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt ttttcaccat
     5881 tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtaa taacatcgga
     5941 gatctcaccg tctttcaatt tgaaagctgc ttcttttact tctgcaggaa tagttgttgt
     6001 cgttgagtcg aatttaactt taccgccatc ttcttttgtc tctgtatcag ttgatttgtc
     6061 tttggctaat ttagagaaat catctccatc atccgctgat tttttcacat cttttgcttc
     6121 atcttcactt gataatttga tgatttgtgc ttctacttct ggatggaaag atttccaagc
     6181 agttttcaaa tcgtcatctg taatatctac gtgagctttc aagccagctt caaaagctaa
     6241 attgttacga atatattctt tataagtatc tttcgtatat ccagcagctt ctaattgtga
     6301 ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat tctgcatcca cttgcttatc
     6361 ggttacttta tcgccatatt tgttattgaa tactttataa atgatcatcc gttgtactaa
     6421 ggattggttc gatgattcaa gttttgcttc atcatagaaa tcagatacag tgatcgttcc
     6481 gcctttcatc gttgcgatat ctttattagt atcgcctgaa caagctgcta gagttaaaac
     6541 agccaacgca ctagtagctg ccaaaataat tgattttttt ttcattaaaa tttgcactcc
     6601 tgtaattagt tattattttt atatattaac tataccatat cccatttcct ttaaaaacgt
     6661 tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca ttcctaggaa atacgcaaac
     6721 ttttctcact tatatagtta aatctgttga gaaaataatc attattatat ttgttcagga
     6781 tactctggca gatcagcttt tagtttttct acttgttcat tgatttgtgt gatacgtggt
     6841 tcggcttgga atttaaaatc ttcgatatct tttttgattg cttcttggaa agcaggaatc
     6901 acagattcag ctgtttcttt tgtttcgatc aatgcgcttt taaaatgatt caggctgtta
     6961 tttaaatcat ttgtcagagt tgttgcttct tccaattctt cgatcagttt ctttcttgtt
     7021 tcattcccgc tcctgggcgc caataacaga cctccgacag tgccagcagc agctccaaat
     7081 aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc tgctttacga ttgtttcttg
     7141 aatagctttt agttcttccg atccataact atcttgatga ttgacaaagt gaatagaaaa
     7201 atcatcctct ttgctgtagc gagggactaa atggatatgt gaatgaaaaa cagattgata
     7261 ggctagttca cggttgttat tcaagatatt aagacctttc atttctggaa aagctttttc
     7321 caatgcttga gcaatcttag gcacacgcga aaaaacctct cctgcaagtt cgggatcata
     7381 ttcaaaaata tctgctacat gacgtttagg cacaactaat gtgtgtcctt tcgttgcttg
     7441 agagatgtct aaaaaagcat agaccttgtc gtcttcatat attttatagc ttgggatttc
     7501 ttgattgatg attttgcaaa aaatacagtt ttccatataa aacatccttt ctataaagaa
     7561 taaatacttc agcttaactt taccataagt taaagctatt gtaaggaaaa aaacaaaata
     7621 ccgtcatttt cttcgtggta taataaataa aaataaatgg cggaggaaaa agaatgagtt
     7681 taattatcga tcatgtcaca ggggggtacg gacatctccc tgtgttaaaa gatatcagtt
     7741 tcgatgtaca gcctggagag atagtagggt tgattggact gaacggagca gggaaaagta
     7801 cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa aggaacgata gaaatcaatc
     7861 atctatcctt aaagaaaaat acggaagaat atcgcaaaca aattgggtat attccagaga
     7921 ctccttcttt atatgaagaa ttgacattga gagaacatat cgaagtcact gcgatggcct
     7981 acgatatacc tatggatata gcaatgaaaa gagcagaaaa gttgttggag acttttcggt
     8041 tatctaatcg tcttgactgg ttcccagcta atttttcaaa gggaatgaaa cagaaagtga
     8101 tgatcttatg cgcctttttg atcgagcctt ctttatatat catcgacgaa ccatttttag
     8161 gcttggatcc actagctatc aatgcattgc ttgaattgat ggtagaaatg aaaaaagaag
     8221 gagccggtat tttgatgtct acgcatattt tagctactgc agaaaaatat tgtgatcgtt
     8281 ttgtggtctt acataacgga gagatacgag cagacggttc tcttgagatg ttgagaaaag
     8341 aatttcagct gccggattct tctttggacg aaatctatat tgctttaaca aaggagcagg
     8401 cacatgagta gtttttttca aaaacgttta gcaagacatc aaaagaaaat gatgaaatat
     8461 atgcgttatg tattgaacga tcattttgtt ttagtttgtc ttttcttgat tggcggtgtg
     8521 ggattatatt attctaattg gttgaaaacc ctgcagccac cgtttgcaat tggagggctc
     8581 ataatctctg tattttggat gctctgttta ttcgtgggta agatggctac ttttgcagag
     8641 gcagcagata ttgttttttt acttcctaaa gaaaaagaaa tgcaaaccta tttggaacga
     8701 gcttttcgct attcttgcat ttttccgttg atctttttga tattggcatg cggattggcc
     8761 atgccattag tggtggttag tacggagcaa tccttttcga tgtttttcgt ctatctgatc
     8821 atattatgga gtttgaaatt cagtcatctg caaatcaaga gagcagaatt gtttcgacta
     8881 gatcaacaag ttattcgtta ttggaaatta ggctggtttt tttcttctct gatctttttg
     8941 tttctatcat tttattggct aatatgggca ggaatagcag gtgcattagt acaggcaagt
     9001 ctatattact ggtttttgtg gaagcaacaa gaaaaaggat tggattggga gaaaatggtg
     9061 catgtagaag aacataggtt gcatcagatc tatcaattta tcaatttatt cacagatgtt
     9121 cctgaaatta cggcaaagat caaaagaaga cgctatttgg atcctatatt gactcatatt
     9181 aaaagagaat caaaaaacac ttatctttac ttgtttgcaa gaagattggc aagaggttct
     9241 gattttagtg gtctttattt ccgattagta attataggtg ggttattgat tgccggagta
     9301 gtcgactttt attttagtct gattatcggt ttcttatttt tatatttgat cggtttccaa
     9361 ctgttacctt tagcgaacca gttccgctat atggttctcg ttcagcttta tccaatccat
     9421 aagaatcaga agaagcaggc gattcagtat ctactcacat gggtattaat tatcgcgtcg
     9481 gttatttttg gtttgattgc tgcagtcgta ttagatgggc agcagcgatg ggttccgcta
     9541 gcggcttatt tgattgttac tatgctcttt gttaaactat atgtaccaac tcgtttgaag
     9601 aagatgtcag actgaaaaca aagctgtgta gggtaaaaat gagaatgaaa aatagagcgt
     9661 tgacagtaag aatgctgttc gttctatttt ttttgagaaa agctggtttt ttgaatggca
     9721 attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg ttcaggcgac tagaataata
     9781 agtactgcaa aacaaagagt aagaggacgg gagaatgcat gatggagttt cagttagacc
     9841 aagagtggcg gttacgtccc atcaaaggcg acacaggaaa aacatacata ggcatgcgtg
     9901 atgaggacaa agtctttatt aaacgaaaca cgactccgat gttggctgct ctttcaaggg
     9961 aaggaattac accaaaactt gtttggacaa aacgaacggg gaatggcgat actttgacag
    10021 cacaagagtg gctggaaggt cgcttgctga ctcctaatga gattggtcaa agaaacgacg
    10081 tgattgatgt tttgtaccat ttacatcatt caaattctct taaaaccatg ctgacccgta
    10141 tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa atatgaaaaa gaattaccgg
    10201 aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg ttttcttcgt gagcagatcc
    10261 cgtcaataga taaggaaaac ttatcagtag tacacggaga tgtaaaccat cgtaattggt
    10321 tagtatgtaa aaattattta tatctagtcg attgggattc aatcatgttt gcagaccctg
    10381 cggtggatat tgcaacgatt ttaggaaatt atgtgccact gtctagctgg aatcagtggc
    10441 tgatcagtta tggcatacgc ccgactgacg aaatgcttga aaaatttcat tggtatgcgg
    10501 ccatgaattt gctccaagaa atcaccagat attgcaaacg tgaagatcat cgacggatga
    10561 acgaagaaat tttacaatta aaacgaattt tcagtggctg aggtttggcc caaactctgc
    10621 ggagatttgg ccagaccttt ttaattagag gaaaggaatg gagataaata atgcgtgtcc
    10681 gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc gaattttgta attagtgatc
    10741 caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa cgatcatcca atccacattg
    10801 agattggtat ggggaaaggc cagttcatca ctggaatggc aaaagcccat cctgaaatca
    10861 attatattgg agtagaaatg caggtcagtg ttgtttcgat tgcactagac aagttgatcg
    10921 aacaaccgct tcctaacctg aaacttctac atgtggatgg atcagcgttg acagagtatt
    10981 tcgctgacag cgaagtagat caaatctatc tgaatttttc cgatccatgg ccaaaaaaac
    11041 gtcatgaaaa acggcgattg acatataaga cttttcttgc tgtagatgaa cagattcttc
    11101 gtccaaatgg agaaatccat ttcaaaacag ataatcaagg attgtttgag tattctctgg
    11161 caagtttttc tcaatatggc atgatcctca aacaggtatg gttagattta catcaaagtc
    11221 aatttgaagg caatattatg acggagtatg aagaaaaatt ttcttcaaaa ggtcaacgta
    11281 tttatcgagt agaagctcaa tttcaagata agaccaaaaa gaaaagtctg tgaaactaac
    11341 tttctgcttt cataaaaaca ttaatatcca taaataaatc tcagaaaatg aacattacag
    11401 atgtaatgta ttttctggga tttatttgat ttaaaatgta tttatttcat cggtttttcc
    11461 acaaggtgga atcaattgga agcgagaaaa tgaaaataat cctttgtcgg tgatcttcaa
    11521 ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa aaaggatcaa aggttttagc
    11581 tgctcctagt tgatgtgctt tagttattaa ttcattcaac tgttcataga cgatcgaaaa
    11641 aggttcctgt gtcatgattc cgcctatagg aagagaaaca caagctattt cctgctgttt
    11701 gttgacaacg gtcatcccgc cacctttttg aatcaaaaga ttagcagcat acagcatatc
    11761 ctcgtcgttt gtcccaacga caactagatt atgcgagtca tgggcaatcg ttgtcgctat
    11821 cgctccttgt ttcagctgaa atcctttcac tatgcctaat ccaatgtttc ccgtttgatg
    11881 gtgacgctcg atcaccgcaa tctttaattg gtctttttcg attgaaggaa tgaatcgttc
    11941 gccttgtcgg tccacttttt ctaccaaatc tttagtcact agactattcg ggataatttc
    12001 gatcacatgg gcttgtgaag aagaaagaag cagctctaac gaatgtggat ttaatggttg
    12061 agcgtttagt tttggcagac aacacgtata tgaatttgaa tgatcagcaa ataaatggtc
    12121 ttggagttgc ccagattgaa caactaatgt tcccttatag aaaacttcat gaattggcag
    12181 cttggtaaga tcgtctgtta agaagaaatc agcttgatac ccaggggcaa tcgcacctag
    12241 tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc atctgaatgg cttgtaatgg
    12301 atcgattcca agtgcaatag cttgacggac gatatgatcg attgatcctt cttttactaa
    12361 atcattgatc agtttatcat cggtgacaaa gaagcatctt cttgaatttt caggtgtgat
    12421 cgccggtagt agcgatgcca gatcttttgc aacggtccct tctcgaacca ttaaatacat
    12481 accaagatcc aaacgttctt tagcttcttt tgcagttgta gcttcatgat ccgtccgaat
    12541 acctgctgca ggataaacat ttaactcttc catgccgata ccagctgcat gcccgtcaat
    12601 ttttttctta tgctggtgca tcaattggat cttttctagg atgtctgttt cattatttgc
    12661 tactgcttga tagttcatga cttcagctaa gcctaaaact tcgggttttt gtaaaaaagg
    12721 ggctaatttt tttgcattta aatttgcacc attggtctca aaaggggtta caggtacaca
    12781 agaaggtagc ataaaaaaga tattcatgag tgtttgtctt gcatcttcta tcataaactc
    12841 aagacctgta ctcccagcaa tgtttcctat ttcatgggga tctgtcacta cagtagtaac
    12901 tccatgaagc aaggatactt ttgaaaattc gcttggtgat aaaagtgagc tttcgatatg
    12961 cacatggcca tcgatgaatc ctggcactag atattgccca ttagcattat gtacgctttt
    13021 tccttcgtat tcaccgattc ctacgatcat tccttcgcag attgctacat ctttttccat
    13081 gatttctttc gtaaagacgt tgacgatctt agcatgcttg atcactaggt cagcaggctt
    13141 ttttttactt gctacttcga tatatttcct taatgttgtt tccaatggag tgcctcccaa
    13201 gataaatttt tataagtata acataaattc ggcttttgcc aatatttttt tgtaaattca
    13261 ttttatttga aacaaaaaga tcaaaaagac gtatgttttt tgtggagggg agaataatgg
    13321 aaataaaacg attatcggat caaatgataa gtgaaagttt tgccaaacat gcccaagacg
    13381 tatatatatt gccagcttca gaagtatttt acatctattt tcgctatgga aaaaaacgaa
    13441 cattctggaa gaaagtctct tcggcagatg gacagcaact gatctcaaga tttaagtatt
    13501 tgggacagat ggatatcgga gaaaaaagaa aagctcagtt aggtgcattt acttattctt
    13561 tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg agattacctt caacgagaga
    13621 gtttagtgat tcggctatta tacggtactt ttgaaacacc ccatcgttgc tttagttctc
    13681 gagattttgc aactatttta cagaaaaccg tcagacgtgg attatacctt ttcagcggac
    13741 cagtcggatc aggcaagact tctctaatgt atcggttggc tttggaagaa aaattgcaag
    13801 tgattactat cgaagatcct gtagagatag aagaaccgtt ttttctgcag ctacagatca
    13861 atccgaaaat agcacagact tatgatacac ttttgaaact cgcattacgg catcgtccag
    13921 acttgctaat cattggcgag ataagagatg aatcaactgc acatgctgcg gtaagagcag
    13981 cactaacagg gcaccgggtt tttgcaaccg tacatgcacg caatcttgaa ggtacattgg
    14041 tcagaatcca tgaactgaca aagaaaaaag aagagctatc tgaatgttta gctggtgtca
    14101 tttatcaaga acttttggaa gatgccaatc accatccttc tgttttatgg agttatcaat
    14161 ttatggagga agaatcagta aaaaaatcat gggaggaaag ttatgaagaa gctcaggaaa
    14221 atatttggca gtcagaacta ttctaaagga ttaacaagaa gccagcaaaa ccagtttgca
    14281 caaaccatgg gagaactgct tttatctggt tttagtctgc aagaatccct ttatttatta
    14341 ctcacgatcc gttcgtttcc acctgttatt ttggcaaata tccaacatca gctaaaggaa
    14401 gggcaagcgt ttagtgaggt actttcgcaa ataggatatg gagcagagca actggtacag
    14461 atcgagttag ctgaacgtca tgggaatctg ccgacaactt tgttggaaat tgccaaacag
    14521 tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc tgcttgcata tccgatgcta
    14581 ttgctcacct ttttgctagg tatcttgttt agtatgcggt tgttcgtttt gcctcagctt
    14641 attggttcag gaatggtcat gcaagagcat tggggtattc agctgataca agtctttcat
    14701 tggtatgttc tactattcat cctagcggtt actctattgg ctttactagt aaggtatcgt
    14761 ctgatgaagt tgtctcctgt tgatcagagt gaatggataa gcagacaatt attgattgga
    14821 ccgatatatt ccttgtatca atcggcttat ttgtcactag agattggaaa gctttttcat
    14881 gaaggtttcg aattaaggca aatcatagtc tgcttaaagg aaacgaaaca agaaagtctg
    14941 attcagcatc ttgcctctaa gttgattcaa ggattggagg caggtatgtc attggctgag
    15001 cagttccaga attatcgttt tctatcagaa gaattcagta aaattgtttt acagggagag
    15061 gcaaagggaa gtttaggtaa agaactgatt ttttacagcg aatggacaag aaaacagctt
    15121 ttcaaacgtc ttcatcactg gatgcatttt attcagccaa tcattttttt gggcgtagcg
    15181 gctttgattt tattagttta tgccgctgtt ttattacctg tttacggaag catagaggag
    15241 gttttgccat gaaaaagaga aaaatatctt attatagcgg ttttacattg atcgaaatgc
    15301 taatcgtctt actgatcatc agtgtattag tgttgttgtt cgtccctaat ttgtcgcgtt
    15361 ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat ccagttggta gacacacaaa
    15421 aagagctcta tgcgcttcaa aataatggaa gagttcctac tgtggaggaa ttgttgaatg
    15481 aaggctatat caaacgagaa catgcagaaa tctatcagcg gccttgaagg taaggtttta
    15541 gcatcaggat atacgttgtt tgaatcactc ttactgattt tattacttac aacgatcctt
    15601 tcttttccag ttctcgcatt ttctgcatgg aagaatgaat tggcagtcta tcaatttttc
    15661 ggccaatttg aaaaacgaat ctattctacg caaaaaatcg ctattgtgaa tcagatccaa
    15721 acaggctttt attggaatag tgaagagaat caaatcattt ttcatgttcc gaatccagat
    15781 aaaataaatt gggaagtatt agatattcct gatgagatta ccttgaaacg ccatgcttct
    15841 attacttttg ctgctagaac cgggaatgaa agttcactaa aagcttatca attttattgg
    15901 gaagaaaaag agcggacgat tacgtatcaa ttccagatag ggagtggtcg ctatacaaaa
    15961 agaattgaat aaaacaggat ttgttttgtt ggagtccctt acagcgctta tgatcgcttc
    16021 tgtctgtgtc tacttagtga gctttggcca gctgtttttg cttcaggcag aaaagaaaag
    16081 tggggaggaa ctgctattta ttcgaatgat ttatgaagat atcaagacgc agcggatgta
    16141 tcacaaaaga ccagagcgaa gggaagaaat gatcccatac gatatccaaa ttgtcgataa
    16201 taatggagta atcgaaaaag tgtttgtcaa tatggagaaa gaagcgatca gtattgaaaa
    16261 aacaagtaat tccagcattt acgctgattg aatgtctaat tggactgatg atattgagca
    16321 gtctattgct cacattcaat cttttgattc agcaaaccat ccaagtaagt gaatttttgc
    16381 agcgtaaaga tcaaaaagag tggctaatat ttttagcaca attagaagaa gaactcaaga
    16441 gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta cacgatcgag gataaacagt
    16501 atatcatcga aagatatcag gcaatgatac gtaaaagaag aacttccggc ggacatcaac
    16561 ctatgttaac aagtatcact gagcttcaat tattggaaaa agaacacaca atttcttttt
    16621 atgtacactt tgaaaatgga gaagatggat atgccatatg gacaaaaaac gatcagtaga
    16681 aaatgttata agggctctat tttattttct gtcttgcttt tatttctttt atgtacgttt
    16741 ttattgatgg cagcattaga aagctaccgt ttgtcaatgg atcttaatat acgaatcaaa
    16801 aattattata tagcaaaaat aatgaagcag atggctttat cggaacagtc tacgcttgcc
    16861 gaaagttcgg aaggagtttt ttactacaca actggatctg taacatatca gtgggtacaa
    16921 cagtccctat tcttagaggt ggaagtctca ccgtttatct ttcggttcac agaagaagta
    16981 aaaaatgata cagatactag tacagaatga tggaatgttc gtgaaaacgc cctcttttct
    17041 gttataacac tgtttttaat aaaaaaatgt ttgccgtttc acaaataaaa aacagtgaaa
    17101 acgattgaaa ttttgtcaat tgttcgatag aattaaacag gtgaaaaaat tgttataata
    17161 tatctagaaa tgaggtgcac catgttacca gaaaaaatgg aacaagggtt taatcaaaac
    17221 ctggaagcta ttcagctttt acagaatgca ttgggaactt cctttttaga agcctatgtc
    17281 gaaaatgcag aaaatttaat agatgattat caagtacgtg tggtggatgg agtgccgaca
    17341 aaagagacaa cgcaacggat cacagctctt tatgaagagt tgaagaaact ttctttcgag
    17401 ccagaagaat ggcgcagact gtctcaatta cttttattga agggcagcca gacagaacat
    17461 ttgcaggcga accatcaact cactcctgat agtatcggtt ttttatttgt ttttcttatt
    17521 gaacaactat acacaaataa aaaagcaccg gttaagattc tggatattgc ggcaggaatg
    17581 ggcaacttgt tattaacagt tcttttaaac ctatcgaatg cggggtacca aacagaagga
    17641 attggtgtgg atattgatga tacattgttg gctgtcgcag cttctacaag tgatttgaca
    17701 caagcaaatg ttcaatactt tcatcaagac gggttgcagg aattattaat agatcctgtc
    17761 gattttgcaa tcagcgattt gccaatcggt tattatccga atgacgaaaa agcaaaagag
    17821 tttttaacaa gtaccgaaga aggccatagt tatgcacatc atttattgct agagcaatca
    17881 atgaaatatg tgaagccgga tggttttggt ttgtttttga tgccgagtgg atttttagaa
    17941 acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag aaggttatct tcaaggaatg
    18001 attcaattgc cagatgaatt gttccgtaat aaacaatcgc aaaaaagtat tttgatctta
    18061 cagaaaaaag gaccacaagc acaacaagta aaagaagtct tgcttgtgaa acttgcttca
    18121 ttaaaagaac cagagaaggt gacggaattt ttcaacgagt ttaaaaactg gaaatcatct
    18181 gctctgtaat cattcaacga gaaggagagt catatgtcta aaacaattgc aatcaatgct
    18241 gggagttcaa gtttgaaatg gcaattatat caaatgccag aagaaaccgt agttgcgaaa
    18301 gggatcgtcg aacgtatcgg tttacaagat tctattttta ccatcaagta cggcgataat
    18361 caaaaattcg aacaaatcat ggatatcgaa aaccacgaag tagcagtaaa aatgctgtta
    18421 gatcagttga ttgaactgca aattttaagc tcttatgatg aaatcaccgg agtagggcac
    18481 cgtgttgttc acggaggaga aacttatggg gattcagtag tgatcgatga gaaagtaatg
    18541 gaccgcattg ctgaattagc agaatttgct cctttgcata atcctgcaaa cttaatgggg
    18601 atcaaagcgt ttaaaaagat tttacctgac attttgagtg tggctgtatt cgatacatct
    18661 ttccactcaa caatgccaaa gcaaaattat ttatatagct taccggtgtc ttattatgaa
    18721 gatttcaaag taagaaaata tggcttccat ggcacaagtc atcgttatgt gtcggaacgt
    18781 gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa tcatcacttg ccacttagga
    18841 aatggtgtat cgatcacagc agtcgatgga ggtaaatcca tggatacttc tatgggattc
    18901 acaccattag ctggggtgac aatgggtaca cgttcaggtg atatcgatcc agctgttctc
    18961 ccatatttaa tggaaaaatt agagattgat attgatgaaa tgatcaatgt tttgaacaag
    19021 aaatctggtc tgctaggcct aacaggattg tctagtgata tgcgtgatct tgaaaagaac
    19081 tatgaaaaag aacatattcg tttagcttat gacattttcg tggaccgcat ccgaaaatac
    19141 atcggcggtt atgtgacaac gatgaatggt gttgatgcaa ttgtctttac tgctggtatc
    19201 ggcgagaatg acggacatgt acgtagtgaa atcatcaaag gtatgtcttg gtttggttgt
    19261 gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag tagatatctc tacaaaagaa
    19321 tcaaaagtaa gagtattagt gatcccaaca gatgaggaac taatgattgc ccgtgatgtg
    19381 gagcgtctgc gcggctaagc caaagcattc gttaggataa aagatgaaac gcatgcaaga
    19441 gggaattctt gcatgtgttt tttttgcgga gaagaccgca aaatggtaat ttaacttcaa
    19501 tttcccttct tttttacagg atcttcttag ggaattttgc ttggaaaagt ggtcaaaagg
    19561 gaaaagactg gcattttatt tattgatttt caggaaaatc ccaatatttt attttcattc
    19621 atctttataa gggacattac atatgtaaaa agaaaaaaca tttttagatc agcctcgtgg
    19681 gctgtcgact ttattcttcg ctttatgttt agctagaaaa ccgatccgaa acattcattg
    19741 atcgaataat aaaaacttga ttttccacct gtactttgcc tgaatcgtga aagcacaggt
    19801 atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc aatcagttgg ccgagcggct
    19861 ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc tgcaaaaagt
    19921 agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt tcttcaattt
    19981 cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg acgttgattt
    20041 tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat aaatctttga
    20101 tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt cgagcgactt
    20161 cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga tcggcaaact
    20221 gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg gtgccaccgt
    20281 aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca ccattgtcgc
    20341 cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg aatagtactt
    20401 gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca gattttccac
    20461 cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg agtgtcagtt
    20521 tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg tctccttcat
    20581 gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct ttttgctgca
    20641 gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga aaacggctgt
    20701 agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt gtggtgactt
    20761 gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta ttctgtaaat
    20821 agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca tcgagtaaaa
    20881 gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt tcgcctccgc
    20941 taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc aagatcgctg
    21001 caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc agctcaccgt
    21061 aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct tcgtagcgac
    21121 tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag tggttgacgg
    21181 tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct tttttgcgac
    21241 tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag gttgatttgc
    21301 cagttccgtt gataccgact aaggcaattt tttcaccagc ttcaatttga caggataaat
    21361 cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt atgatcatga
    21421 tctctctcct tgagaccact caaaaaggcc atggttatcc ctggcctgtg ttatttaaac
    21481 tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga attcaactgt
    21541 tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc taaatgcgat
    21601 atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca tagtattttc
    21661 cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt tcaactagcg
    21721 ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt tttatctgac
    21781 tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc gttcaggcga
    21841 aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc atttgcttaa
    21901 taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg ttcaggagta
    21961 tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga tttaccgaat
    22021 gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt ggcgataaaa
    22081 tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga aacttccaca
    22141 ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca gggcgactaa
    22201 tcgccctggt gttcactttt tttattttac ttgaactcat tgactgtttt ccctttattt
    22261 taaacattat aaagaccgat gagtgcttgc cgcgtctttt gtttttatca cggttttttg
    22321 tgcaaaaaaa cctgccgata gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt
    22381 tagctggcaa ttggttttct aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc
    22441 taataacata ttcagaaaca gaaccaataa ataaacgttc tacagcattc aatcctgtgg
    22501 ctcctaacat aatcaaatct acttgattat cttctgggat ttctctggca ataatttgtt
    22561 ttggtgagcc atattctacg acagaggtta cattattcaa gccagctttc ttcgcattgc
    22621 tttcataatc tgccaatgtt tgctttgcca tttcagtagc ttgttctgca agcattccat
    22681 caaaggatga aacggtttgg aaagaacgtg tgtcaatgac atgtgctaat agaagttctc
    22741 cgttatttcg gatagctaca ttgaccgcct ttttaaaagc taattcagct tcatcagaac
    22801 catctacggc aaccatgatt tttttgtatt gttgtaacat aagcattcac tcctcaagtc
    22861 aatcgtattg tactctttta ttgtagaaca ctttagggaa aaagagaatt atttactgct
    22921 ttggttcagc aatttttttt gcaaatcgga tactgaaggt ggcagaaaca ataattacga
    22981 tcagtaaaaa gaccaaggca ataaagcgtt gattgaaaaa agctgttaaa atacccatta
    23041 cgcctaaaac agcatatgca gcaccgtaaa attgtaggaa tccttgattt ttatcatttt
    23101 tgttgatcaa tacaaaaaag atatcctgtt tattaaataa ataatagcta acgaataata
    23161 atactaaagt ggcgataatc attaaaatcc taatcatgaa caaatcctcc aaaataagca
    23221 acaaatagaa aaagcaattt gccaagagca aattgcttta ttctttaaaa gatggtaatc
    23281 tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc
    23341 catggtcgta gcttcgaact accaaatgat aaggcatgtt accagcagtg acacaagacg
    23401 ggtcccaaga tatcaataaa aatgttcggt caacacacaa agaggacaat tcgaacatca
    23461 cagatttccc acctctatag tagcatatga atttaaaaag agcaatgaga gagctcgcag
    23521 aaacagaacc gttttcttgt ctagtttttt ggaggatgct ttttccactc ttttatacgg
    23581 taatactgtt gacccagagc ctgttcatat ttccctgtat cttttggctg ataatactga
    23641 gcattcttca gtttatctgg taaatattgc tgattgaccc aagcctgatc aaaatgatga
    23701 ggatactgat aacccacgcc acggttgagt gatttcgcac ctttgtaatg gctatctctc
    23761 aaatgatcag gaacatctcc agctttgccc tctcgaatat cagcaagtgc tgcatctaat
    23821 gccatgtagg cagaattgga ttttggcgac aagcaaagat cgacgacgac gtctgctagt
    23881 gggatacgtg cctcaggcaa tcccaatttt tcagcagcta atacagcgtt cactgtacga
    23941 gccgcagcag caggattacc taaacctata tcttcataac cgataaccat cagacgccgg
    24001 caaatacttg ccagatcacc tgcctcaact aaacgggcga gataatgaag tgcagcatcc
    24061 acatcacttc cacgaatcga tttttgaaaa gccgaaataa cgtcataatg agcatctcca
    24121 tttttgtcat gggtaagagc ttttctttgg acacattctt caatgatcga cagtgtaaga
    24181 tgaatccttc cttctttatc tgaaggagtc gatagtgtag ccagttccag cccattcaat
    24241 gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac
    24301 tggattgctt gttgtccaag gccgcgttct ttatccctta aagcatgttc gactgccaac
    24361 tggatgtctt gttctgttaa tggtttgact tcaaagatct gtgtacggct gcggatcgct
    24421 ggattgatgg taatataagg attctcagta gtcgctccga tcaagatgat tcggccactt
    24481 tctaaatgag gaagcagaaa atcctgtttt gttttatcta atcggtgtac ttcatccaat
    24541 aagaggatca ctgtaccact catttttgct tcttccgcta caacttgtag atcctttttt
    24601 gaatctgtag cggcgtttag catgcgaaat gcataattag tagagccggc gatcgcactt
    24661 gcgatactcg tttttccagt accaggaggt ccatatagaa tcatggaaga gagcatgcga
    24721 gcatcgacca tgcggcgtat gatttttcct tccccgacta gatgctgctg accaacgact
    24781 tcatcgatcg ttcgaggtct catgcgataa gccaatggct gttgcatagg attttcctcc
    24841 ttcttgtttt tacgtccctt aattatatca tatttagcaa gatgcctaga aagaacagcc
    24901 ttctgttgtt ttgttttcat agcttatcca actaatattg tgtatgataa agtaggtgag
    24961 aaaaatgacg gataaacaaa agatagatga atttttttgg aataaaacga cgccagaagt
    25021 ggcacgggat ttattgggga tgtatttgga gcatgacaca ccagaaggaa gattagctgg
    25081 atatattgta gacgcggaag catacctagg tccagaagac gaggccgccc atagctatgg
    25141 tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta
    25201 cacgatgcat actcatcgga ttttgaacat cattactcag ccagaaggga ttcctcaagg
    25261 agtaatgata cgtgcaatcg aacccgccgc aatgattgat caaatgagta aaaatcgtgg
    25321 cggaaaaact ggtccagata tcagcaacgg tccgggaaaa ttagtggaag cattagcaat
    25381 tcctcaagaa ctttatggtc agtctatagc agatagttcg ttacgcttag tttttgagaa
    25441 aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt
    25501 atggacagaa aagccgttgc gttttgttgt gtcgggaaat ccttatatct ctttacaaag
    25561 aaaaaaccaa atcgagaaag attggggttg gaggaaagaa aatgaaaaag aaggatctat
    25621 taacatattt agatgaaata attgaaaaac aagtgacaga ctatgatgta gctttagatt
    25681 gggatacaaa aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa aataaagcac
    25741 aatttgagat tgatgatgca gaagggatcg tttcgcaaga accaattatc gaatttgaag
    25801 acggcgtact tctttttaat cctcagaaat ctgtttttga tgaacgcaat tatctagcag
    25861 taatccctta tgaaggaaaa aaagggttgg caaaatcagt agctgattct ttagtggaat
    25921 atctaaatga agtattagct caagggcaaa gcgatttgct tgattttttg gatgaagacg
    25981 atgacgaagc tgtattcgag cttcactggg ataatcaaca actagcagaa ttagtagaag
    26041 taaaacagca aaatgaagca gatacttatt taccgtaccc aagttattaa ggagtgacag
    26101 atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta
    26161 atattttgat ggaggcaggg gcgagtggtg tcgcgattga agattcttta gatgttgaac
    26221 attttcagtc ggatcaatat ggtgagattt tagataaaaa tgattttaca tcgctgaaag
    26281 aaggcgcact agtcatggct tattttccgg aaacgacttt cttaccggaa atcttgcctt
    26341 ttatcaaaga tcgtatcaca caattacctg agtttggtct agctattgga aaaaataaag
    26401 taacagttag tgaagtagag gaaagcaact gggcaacagc ctggaagaaa tactatcatc
    26461 cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg ggaaaactac cagacagaag
    26521 atcctctaga aaaaattatc acactagatc ctggtatggc atttggcaca ggcactcatc
    26581 ccacgacttc cttgacattg acagcactag aaacagtctt gcgtggtggt gaaacattgc
    26641 tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag
    26701 atgtctatgc ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa aatatggata
    26761 tgaatgaaat agccaaagat gtccatgtat cagctaacga tcttttgaca ggaatcgaga
    26821 ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat catcttattg atgatcccag
    26881 atgcttggcg tttactgaag caaacaggca cgctgatcgt ttcaggaatc attgaagaga
    26941 agaagcaaat ggttctggat gccatgttag aacaagggtt tatcgtggat caaattttcc
    27001 agcaaaaaga ctggtatgca attattttga aaaaaccaga ggtggaatag atgcagcggt
    27061 attttttaaa agaatcatat gaatcaaaag actattttgt tttagaaggg gaaaactatc
    27121 accacattgt acgggttatg aggatggaac ccgaggatga agtttttttg gcattcaatg
    27181 atcaaatagc cattgttgca gaaatcacgg agattactga agaggctgtt tatctgaaag
    27241 aaattaaaaa agaagaaaat caaaaggaac tgccaagttc gattaccata gcgagtggct
    27301 atcctaaagg ggaaaagctg gattggatcg ttcagaaatc cactgaatta ggagcgcaag
    27361 cttttcttgg tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga
    27421 aacgacaaca acggcttgaa aagattgctc aagaggcagc ggaacaatcc catcggcaga
    27481 tcgtgccatc agtagagtta ttggaaacga aacaagattt atttgagcgg ctagatgatt
    27541 atgacgtggt cttggttgct tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag
    27601 ccaaaattct tagtgaaaca aaaccagata gtcgaatctt agctgtcttt ggaccagaag
    27661 gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca caaagggata ctttgtggat
    27721 taggaccaag aatccttcgt acagaaacag cacctcttta tttattaagt gctgctagct
    27781 atcaatggga attaatgaat ccttctttca gctaacgttc ttcattaata aaagagtatc
    27841 gacagaaaga attgaaaaaa tagtcaacaa tttgtataat agaaacaatt atttatacaa
    27901 tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt
    27961 ataactgcac ggattttggg acaattatca aacagatatt tgtcccgatt cccgttttac
    28021 cgaaaaaaga aagggggatc gattcctgtg acaaaagaag aaattttaac gggacccggt
    28081 gtcataaagc tcgttagtca atacatgggt cccgaacatg tagcatttgt tgagaaagca
    28141 tgcgaatacg cgactgctgc acatgacgga cagtttagaa aatcaggcga accctacatt
    28201 attcatccta tccaagtcgc aggtatatta gcagatttaa aaatggatcc ccatacagtg
    28261 gctacaggct tcttacatga tgttgttgaa gatacagaaa tcactttaga agatctgaga
    28321 gaagaatttg gcgatgacgt tgctatgtta gtggacggcg taaccaaatt agggaaaata
    28381 aaatataaat cccacgaaga gcagctagca gaaaaccacc gaaagatgct gctagcaatg
    28441 gctcaagatt tacgagttat catggtcaaa ttagctgata gactacataa catgcgtacg
    28501 ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc aggaaacttt agaaatctat
    28561 gcgcctcttg cacatcgtct agggatcagc cggatcaaat gggaattaga agatacagca
    28621 cttcgttatc taaatccaaa acagtattac cgtatcgtcc acttgatgca gacgaagaga
    28681 gaagaacgtg aaaaatacgt aagtggtact gttgaagata ttcgaatagc gacagaagag
    28741 ctggggattt ttgcagaaat ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa
    28801 atgaaagatc agaaaaagca gttcaacgaa atttatgact tgctagctat tcgagtgatc
    28861 gtagattcaa tcaaggattg ttatgctgta ttaggagcaa tccacacaaa atggaaacca
    28921 atgcccggca gatttaagga ttatattgcc atgccaaaag ctaatatgta ccaatctttg
    28981 catactaccg tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa
    29041 atgcatgaaa tcgctgaatt cggggttgct gcacactggg cctataaaga aggaaaaaat
    29101 gaaaaagtag aaccagatgg tatgacgaaa caattaagct ggttccatga gatactcgaa
    29161 cttcaagacg aaagctatga tgcttctgaa tttatggaag gcgtaaaagg agatatcttt
    29221 agtgataaag tctacgtctt cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc
    29281 ggaccattgg actttgcata cagtatccat acagatatcg gtaacaaaac cactggtgca
    29341 aaagtaaatg gcaaaatggt gcagcttgat tacaaattga aaaacggaga tatcattgag
    29401 atcatgactt ctccaaattc atttggccca agtcgcgact ggttgaaatt agttgctact
    29461 agcaaagcaa gaaataagat caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg
    29521 atcaaaggcc acgaatccgt ggtcaaatgt attacagatc taggatttac gcctaaagat
    29581 attttgacga agaacaaact gcaagaagca ctcgatcgtt ttaattatca aacagaagat
    29641 gatctctatg cagctgtagg gtatggagaa gttagcccct tgacgatggc caatcgtctg
    29701 actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc
    29761 atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc
    29821 attcaagggg tagaaaactt actaattcgt atcagtcgct gctgtaatcc gattcctggt
    29881 gatgatatcg ttggttatat cactaaaggc agagggatat ccattcatcg tcgagattgt
    29941 ccgaatgttc agcctgacaa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa
    30001 gatacatcga atacacgaaa agagtatgat gcagatttgg aaatttacgg ctataatcgt
    30061 tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc
    30121 gttgaagcac ggacgaataa agataaaatg gcgacgatcc atttgacggt tggtatccag
    30181 aatttatccc acctaaagag tatcgtggat aaaatcaaag cagtacctga tgtctacagt
    30241 gtacgccgga cgaatggata ggaggacaga tatgagagca gttgttcaac gggtaagtaa
    30301 agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc tacttgtttt
    30361 attaggcatt catgaaaccg atacgcaaaa agatgtggac tatctggtaa aaaaaattgc
    30421 acaaatgcgt atttttgaag atgaacaagg aaaaatgaat attagtgtag aagatgtggg
    30481 aggacaaatt ttaagtatct ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg
    30541 accaagcttt gtggaagccg cacgaccaga aacagctatt cctttatacg aatcattcaa
    30601 tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga caattcggag cagatatgtc
    30661 ggtttctctc gtcaatgatg gacctgtaac gatcattatt gattctaaaa acaaataaag
    30721 taatctggtt ttcactagta aaaactatga aacacgtcgc ttaagttaga ctgataaact
    30781 ctaacttaag tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa
    30841 agttaagctt aaattataca agttattttt tataggaggt tgctgaagat gaaagaagta
    30901 ccagtagtag atatcaaaat cacagattta aaaaaattgt atcaaaaagg atacaacatg
    30961 ttggaggagt tacgccatga agcggacgct cccgtcgtca aagcaaagat tttcaataaa
    31021 gaagctatca cgatctacgg atcatcagca gcaaaagtat tttatgatcc aaggaatttc
    31081 aaacgaaaag gtgctatgcc aaaattagta ttgaagacac tttttggtca aggtggagta
    31141 cagactttgg atggagctgc tcatcaccat cggaaaaata tttttatgga tctaatgaca
    31201 ccggaaagaa tggaagatta tcatcgtatc ttagataaga atctgacaca agcacttgaa
    31261 gcacagcatg gtcaatttga actatttgat ttaagtaaga tggttttctt tacatctatt
    31321 tgcgagtggg caggtatcaa tttatcagcg atttctaaag atgaggtcga aaaattagca
    31381 gaatatcaaa tctctatgat cagcggaacg tttacttctc cgattgatca cataaaaggt
    31441 gtcgagaatc gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa
    31501 aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc cacagattta
    31561 gatggtcagc ttttacctct ggaagtggca gctgttgaat tattgaacat catccgtcca
    31621 acggttgctc taactgtgtg ggcggcattg atgggacatg cacttttcag tagaccagat
    31681 ttgtatcagc aattaaaaaa tgacttttcg acgttacaag atccgtttat tcaagaaatg
    31741 agacgctatt atcctttctt cccaatgcta cctgcgattt cgctgaaaga agtagaagta
    31801 gatggttatc gtataccaga aggcagttgg gttattttag atttatatgg aacagaccat
    31861 gatgaacgaa cagttgaagc gccagattcc tttatgatca aacggtatgt agggaaagca
    31921 aaagatattt cctataaaga agaatatgag atgattgcac aaggcggggg aaattttcga
    31981 caaatgcatc gctgtgcagg agaatggatc actcttcata gtctacgtgt gttttccgat
    32041 cagctagtaa ataagtttga gttctctgta cctgaacaag attggacgat tccattcaat
    32101 cagtttccga cttatccgaa tagccgagcg ctactttaca agaattagat tagcataaac
    32161 ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct tttttcatat tactataaat
    32221 tgataaggtc aattgatcaa aatccacgaa atgcttcatc aatggtttga taatcttcat
    32281 tggataaatg caaatctaaa gcttggacat tgtcagatac ttgctctgga cgcttagctc
    32341 caggtataac tacagacact cgtggattct tcatgtacca agctaaaaca agttgcgcag
    32401 gagtggcttg gtatctttta gcaatggggc gaagtacatc gacagcggtt aaaatttctc
    32461 tgaaacgttc tccttggaaa tctggatttc tttttcgagg atctccttca ccaaattgtt
    32521 tttcctcacc aagttcatat tttcctgtca gcaaaccgga agcaagagga aaatagggaa
    32581 caaaactgat tttgtttttt tcaagataag ggaataattc tttttcagcc tgccgatgga
    32641 tcaaactgta tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt
    32701 gttctaacgt gaagtttgac acaccgactg ctcggatttt tcctgcttct tttaattcgt
    32761 gcaatgtagc aactgattca ttttttggtg tgctttcatc cggaaagtgg atataaaaaa
    32821 tgtcgatgta atcagtttgc agacgtttta gtgcatcttc caccgattgt ttgagaaact
    32881 caggagaatt atcaaatgtt cgccctttat ttggtacatg agcagctttt gtagcgatga
    32941 cgacacgtga gcgatcatat tctttttcct tcagtacctc gccaatcagt tcttcggaac
    33001 ggccgtttcc gtaagcataa gcggtatcga tcaaattgat cccgctattc aaagctgtac
    33061 gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt cctccaacgg
    33121 catttgctcc taatccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg
    33181 ccatagctga ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa
    33241 acgtttttgt gaggggaaat agtcagatat ctcttgactt tgagactggt ttttagtagg
    33301 ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact ttacaagaga
    33361 ggatcatcga ggctgaaaat gatctaaagt ttcattagcc taaagacact ctggagatgg
    33421 acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag aaatctttca
    33481 aaattaggtg gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa caagggtgat
    33541 ttttttattt acatagctaa gaaagggaga gaaaactatg agtttccaaa gaccaaaagg
    33601 aacaaatgat attttgccag gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg
    33661 tttattattc agagattatc agtataatga aatgcgtaca ccgatttttg aacactatga
    33721 agtgatatcc cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga tgtatgattt
    33781 ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac caatcgttcg
    33841 ttcttttgtt gagcataaat tgttcggccc agagtatgcg aaaccgttca aaacttttta
    33901 tatgggacca atgtttcgct acgaacgccc tcaaaaagga cggttacgtc aattccatca
    33961 aatcggagta gaagcatttg gatcagaaaa ccctgcaact gatgtggaga gcatggtgat
    34021 ggcacttgat ttcttcaaac agttggggat cacgcaaatc cggctagtca tcaattcatt
    34081 aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg atcgactatc ttttaccttt
    34141 tgaatcagag ctgagtgaag actctaaacg ccgccttcat gaaaatccat tacgcgtatt
    34201 ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa gctccatcta tcttagatta
    34261 tctaagcgag cctgctcaaa aacattttga taccgttgtt tccatgctgg atgcattggc
    34321 tgttccttat gaaatcgaca gtaatatggt tcgcggacta gattattaca cacatacgat
    34381 ttttgaaatc atgagtgacg caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg
    34441 acgttatgac aatctagtgg aagaactagg tggtccttca acacctggct tcggatttgc
    34501 aatgggtatc gagcgattac tcttgactat ggaggcagag gaagtagtta tccctgcatt
    34561 taatgagttg gatgcttacg tggtcgcact cggagacgaa acaaatatcg aagcattgaa
    34621 agtggtccaa gcgattcgaa actttggttt ttcagctgac cgcgacttca tgaaccgtaa
    34681 agcaaaagcg caatttaaga cagcagataa agcgaatgcc aaattagtat tggtgatcgg
    34741 tgaagatgag cttgctagtc aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa
    34801 aagttttcca ttagaagaga tatatgaaca ttttgatgaa gtctatgatg aaatgacttt
    34861 attcgagtag gaggaaaaaa ataaatggca caaagaacga tctattgcgg attagtttcc
    34921 gaacaacaat tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt
    34981 gggggagtaa tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat
    35041 cctgaaatca acaaagaagc atgggaaatc gcggataaat gtcgcagtga atacgtgatc
    35101 gaagtgactg gggaagttag aagacgcgga gaaaatgcag taaatcctaa aatggccact
    35161 ggtgaattcg aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aaccccgcca
    35221 tttcaaattg aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg
    35281 gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg
    35341 attcgtcatt atttagatga taatgacttt ctcgatatcg aaacacctta cctagggaaa
    35401 tctacaccag aaggcgcaag agactaccta gttccttcac gtgtccatgc tggacatttc
    35461 tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc tggattcgac
    35521 cgttattatc aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct
    35581 gaatttacgc aagtagatat tgaaacaacg ttcttaacag cagaagaaat ccaaacttac
    35641 acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca
    35701 tttcctcgta tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca
    35761 cgttttgata tggaattgat cgatctaagc gatacagtca aagaagtaga attcaaagtc
    35821 ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca
    35881 gatcgctact cacgaaaaga catggatcag ttaggtcaat atgtaggaca attcggagct
    35941 aaggggcttg catggctgaa agtagaagaa gacggattga aaggtccaat tgctaaattc
    36001 atgggagaag caacagaagc aatcattaaa gcaacagatg caaaaccagg tgacttgctg
    36061 atgtttggcg cagataaaag tgaaatcgtc gctgctgcat taggtgctat ccggacacgt
    36121 ttaggaaaag aattaggact aattgatgaa tcgaaattca atttcttatg ggtaactgat
    36181 tggccacaat ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc
    36241 acgatgccaa aagaagaaga tatcccgtta ttggctgaag accctgcaaa agtctatgca
    36301 gaagcttatg atatcgtctt gaacggttat gaattaggcg gaggttctat ccgtattcat
    36361 actcgtgagc ttcaagaaaa agtactagaa actctaggct ttacaaaaga acaaatggaa
    36421 gaacaatttg gtttcttgtt aacagcttta gattacggat tccctccaca tggcggtatt
    36481 gcattaggat tggaccgttt tgcaatgtta ttagccggag aagataatat tcgtgaagtc
    36541 attgcctttc ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc aagtatggtt
    36601 tcgcctttac aattgttcga attgaatatt gatgtaacga atatagaaga atagccgata
    36661 aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca accttgattt aattgaaaaa
    36721 cactttataa tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa
    36781 aattgggctt atcatgatta cacgaccaag ctttcggtat ctatcgttta tgcgttgatc
    36841 gcatcagtcg cattgaattt cttttatcag ccaggacata tttattcaag tggtattact
    36901 ggtctagctc agattttgac gactttgtca aaatcactga taggattcga agtaccggta
    36961 tcaacaactt tgtatttact gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg
    37021 aagaagttta caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt
    37081 ttaccggaaa cagtcctatc taccgatcca atcatctgtg cgttgtttgg gggggctgtg
    37141 atgggactag gtattgggct agcgttgaga aacggcctat catcgggcgg tctagatatc
    37201 gtgagtatca cgattcgtaa aaaaacagga aagtcgattg gttctatttc gatctacttc
    37261 aatgcattga tcgtttttgt tgccggaatt ctatttggct ggcaatatat gttttacagt
    37321 acgttgtcta ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaag
    37381 atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt
    37441 atgcgtagag ggatcacgat catcaatgaa gcagaaggtg cttataagca tgataagcag
    37501 acagtattga tgacgatcgt tacacgatac gaactgcctc tacttagaga agcaatgaaa
    37561 gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg tccagatact tggtcgtttc
    37621 tatgaggatg gcatttagat cgagtttgac aaaaattcat gaaatactgc tgtagacaga
    37681 cgttcgtttg tctgcagttt ttttctacca tagtagaatc agcaaagaaa aactgctttt
    37741 ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa tttatgggat
    37801 gatagaaaaa aatagtagag aaaggacacg attctacttg ctattttcac ttaatacccg
    37861 tatattacga atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag
    37921 gttaaaaaac agccgctaca tcactgggtt tgatgggctc cgttcattag ctgtggtggg
    37981 tgttatcttc tatcaccttc tgccaactag tatgaaaggc ggctatttgg gcgttccgat
    38041 ttttttcgtc gtatctggtt atttgatcac ggatctgctg cggcaggaat gggaacaaaa
    38101 tggaaaaatc aatatttggc aattttatgt cagaagaatg aagagactct atccggcact
    38161 tgtttttatg cttgtgacag cttcggctta tatcactttg tttcagcgag ggctgttaaa
    38221 caatcttcga ggagttgtgg tcagcagctt actgtatgtc aacaactggt ggcaaatcaa
    38281 taacggttta tcttatttcg atcgatttgc taatgagtca ccgtttacgc atatctggtc
    38341 tttagcagta gaaggtcaaa actatttggt ttggccgatc ttgtttgtat tgttgatggt
    38401 atttgttcgt aaaagaaaat ggatctttta tactgtgtta ggtacatcct tcctctccgc
    38461 catcttgatg atgatcttgt atacaccagg aggcgatcca acccgtgttt attacggtac
    38521 cgacactcgt ctgttttcta tatggttggg aagtgcgctt gcgtttgttt ggccaagtac
    38581 gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat
    38641 ctcgcttttg gcactgattc tttgcttctt ctttttagat gatcattaca atttcatcta
    38701 ttacggagga atgctcctag tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca
    38761 ccccggagca agtttgaatc gatggctgac aaatccagta tttacttgga ttggcaaacg
    38821 aagctatggc atttatctgt atcagtttcc agttatgatt ttttatgaag caaaggtgac
    38881 tgatattgcc gatcatgttt tgttgcatac tttagtggaa atcacattga ttctaatcat
    38941 cagtgaactt tcttatcgat atgtggagcg tccgctagca cgttttgatt atggtcgtac
    39001 attccaagtg atgaaaggat ggttcagcaa accgatcatc agtaagagca aaccatgggt
    39061 tgttccaggc acattacttg tagcaattgc actcgttggt ttcgttacgg caccgaaaaa
    39121 cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta aaaaattagc
    39181 agaagaaacc agaaacaatt cagaaaacac cactagttcg gttgaccaag cgatccttga
    39241 taagtacgag ttgtccaagg cacaagggaa taaggcacaa aaattagaac taacggcatt
    39301 tggtgattca gttatgttgg atgctgcaac ggatctacaa gaactttatc caaaagtagt
    39361 ggtagatgga gatgtcggac gacaattata tgccagtgta ccgtacattg aaaaattaaa
    39421 ggaacaagat ttgttgaaag acactgttct gattggacta ggaacgaatg gctcattctc
    39481 ggaagctcag tttgatagtg tgatggatgc aatcggtgat cgaaaagtat actgggtcaa
    39541 tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac
    39601 gaaaaaatac gacaatatga cgctgattga ttggtacagc tacagtaatg accatagtga
    39661 ctggttttat gatgatcaag tccatccaaa tcctgaaggg atgacacatt acattcactt
    39721 agtcagcgaa gccttgctag gaggaagttc aagtacgaac agcagtgaga caaacgtgac
    39781 agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga agttttttta
    39841 tatttaccgt gtttttaaca ttcatataga ttcaggaagc aaaacggttg agtgtgtctg
    39901 taaataacgt tctttttgtt ttcgggtgag ttttttgaat gcagcttctg cttttgttgc
    39961 ttcgcttctt gaagaaaata cttctgcatg gatcatctta acaggtcttc ttttttgaag
    40021 acgagtatat ttagcaccag taccattgtt atgttcctct aatcgtctag aaagatcggt
    40081 tgtatatcca ccataaaatg tctgatcttt acagaataat acgtaaaaat aatgttcatt
    40141 tgccatagag catctcacga atctctgcag tgtattctcc atccatattg taagtgatca
    40201 atggtggagc gactttgaat ccgtctaatt tgccttcttt gattccttca actaacaaaa
    40261 tatttgcctc ttttccaggt tttgggtaaa caaattggat ccttttaggt gcaattcgat
    40321 agtcttccat gacctgaatg atttctaaga accgatcagg acgatggacc atagtaaagc
    40381 gtccatttgt ttttagtagc ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa
    40441 tctcatgacg ggcgattgcc aggtgtttat tagggttcgt tttatttgtt ggcagccctt
    40501 tgaaataagg tggattacag acgactaagt cacaagaatt atgtcgaacg acagataagc
    40561 tgtctttcag atcgatggtg tggacagtca tttgttcatt taaatggtta agcgcgatac
    40621 tacgtcttgc catgtctgct agacgaggtt gaagttcgat agcgtcgata gccgcctggg
    40681 ttcgtttact taaaaaaagt ccaactgcgc catttcctgc acaaaggtca acaatcttcc
    40741 cttttttagg tacacgggaa aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca
    40801 ctttggaact ttggataatc ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt
    40861 cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg tctgtattat actattttta
    40921 ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt atgcatacta tgaccataag
    40981 aagggaaagg aagagactat gttttatcga tttatgcgcg gggtagttcg agtactatta
    41041 gccttagtta atggaaatgc ccgctatgag aataaagaag ttttaccgca agatgagaat
    41101 tacatactag ttgctcccca tcgtacttgg tgggacccat tatatctggc ggtagctgca
    41161 gcacccaagg aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga
    41221 tttatattaa ctcgatccaa tgcttttcca gttaaaagag aaaatccagg accaagcgtc
    41281 attaaaacac ctgtaaagat tttgaaatcg acaaatttga gtttgatcat gtttcccagt
    41341 gggacaagac atgctacaga acttaaaggc ggaatggcat tgatttccag aatgtctaaa
    41401 gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac ttggggatct gttcaagcgt
    41461 cagagagtga cagtgcgttt cggcgaacca atcgacttat cagatatcaa gaaaacggat
    41521 aaagaaggta tggcggagat cgaacgacga acacaagcag cttttgacaa attggacaaa
    41581 gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt
    41641 gggcggtctt ttgaatctac tgtcctgttc agttcagact ttttcaatga aaacgaggga
    41701 gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta aaatagaaga caggtgtcat
    41761 gtaatggatc aaagtgttat aataattaga aggacatttt ttgaggtgat tgaatgcgtt
    41821 ttttacatac agccgattgg catatcggaa aaaaactaca aggttatgac ttgattgaag
    41881 accagcgtca tgtactatct tctatactag atatcgcgaa agaagaaaaa gtagaggcaa
    41941 tcgtgattgc cggagactta tatgatagga gtgtcccagc tgttgatgcc gtagaattat
    42001 acaatgaatt gatggtcgat tggaatttaa aggaaaagat cccgattttt gctatatcag
    42061 gaaatcacga tagcagtacg agactttcag cgggtacgcc atggttcagt tattcgaact
    42121 attttttgcg aacacagcta gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat
    42181 ttttctttct tccttacttt gaaccaattg cagcgagatt atattttgaa gatgacaata
    42241 tccgaacgat caagcaagcg gtagccaagg tgatagataa aatgcaggag ctatttgatt
    42301 cagaaaaaaa acaagtattg attagccatt tttttgtaat gggaagtgta aaatctgatt
    42361 cggaaacgaa aatcgaagta ggcgggttgg attcgatccc tggtgaacta cttacaccgt
    42421 ttgattatgt agcactagga catttgcata atcaagctgc tttgagacag gataatgctc
    42481 gctatagcgg ttccccattg aaattctctt tatctgagat caaccaaaaa aaaggcgtat
    42541 ggatcgttga cctgacaaca ggcctggagt tgaattttag ggagatcgaa cccctgcatg
    42601 atattgaaga aataacagcg agtttcaaag aattgctgga tcctgccttt tatcagtccg
    42661 tcaatcggga gaattacatc tatatctatc tcactgatag aacgatcatc ccgaatatga
    42721 tgaaccagct tcggcaaata tatcctcgta ttttggggat tgaacgggca aacggtcgag
    42781 cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa aaattagcag
    42841 aagcattttt tagtgaaatg acggaagagc agctaactgc aaaccaagaa aaatggctcg
    42901 gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg aaaaaattac
    42961 aacttaaaaa ttttggtcct tttatcaatg agaccgtaga tttttcacgt ttgacagaag
    43021 caccgctttt tttgatcagc gggaagacag gagctggcaa aacgacgatt tttgatggga
    43081 tcaccttcgc actttttggt gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt
    43141 ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga
    43201 tgagctatga aatcgaacga aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa
    43261 caaaaaaaca ggcagccaaa gtaacgttga ccatttttga tgggaaagga aaagaaataa
    43321 aacaaatcac aaaacgtaca gaagcagatc agttgatcag agaactgatg aatctcgatg
    43381 cgaaacagtt ttctcaaatc gttttactac cccaaggaga attccgtaat tttttggttt
    43441 catcaagcag tgataaagag actgttttac gtcatttatt cggtactcaa tttttccaac
    43501 agttcaatga gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg gatcatttag
    43561 agcaagagct gcatcttctg caaaaaaggt ttgttttagt caaagaagag gaaccagcag
    43621 cagggtttga agccgtttta gcccaatgga caaaccatca ggcaatactc gaaaaacaaa
    43681 tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt
    43741 cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca
    43801 agcaaaagga attagctgct caaaaagacg aaatagagga aaagaaacga tggattgggt
    43861 attatgaatt tacgcaacga ttagtcgaac caatccagcg cctacaagaa ataaaagttg
    43921 aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg atgactacgc
    43981 agaattatga agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca
    44041 aagaagctgc tcaaaaactg caaaacaaac agatgatggt gccaattgtc gaatccttac
    44101 gtagtaaaaa acaagacgca gaacaattaa caatttcatt ggataaagaa gaattgaatg
    44161 ccgagaagct gtatcaaaat ctcgctatct atcaggaaag aactcgaatt cttcaagaaa
    44221 atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc
    44281 tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa agacactggg tcgctcgcac
    44341 agataagaga agaactcaaa aaagcaactg aagaaataac aaaaaatagt cagctattga
    44401 aagaacagac agaacaagca gaacagctga aaagccaatg ggcaaagcag caaattgctc
    44461 gtcttcagct ttttttgcgt ccaggggagc catgtcttgt ttgtggatca acggaacacc
    44521 ccatacaaca attaaaacac gaacagccaa ataaggaaga tatcttaaaa gtagaacaac
    44581 aactggcaga agctgagtct gctgtagaaa gaactcagca aatcattgcc aaaagtgaag
    44641 cacatgaaaa taatcttcaa caacaattat cagatttaga acaaaaaatc atggcaacga
    44701 atatacaatt ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag gcatttcctg
    44761 agataacaga acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct ttagatagac
    44821 gaaaaagcaa gatggccaat gcaagaatag agctggaaga aataagacaa caagcagaaa
    44881 agctcgaaca acaattagct gtctacaaac agcagcagac agaaaaaaat aatcagctcc
    44941 agcaactgca aggagaaatc aaaagtttag aacaacaatt gcaagatacg gatagtaact
    45001 gtttagaaga ggagatacga cgacttgaaa aacagttaga agaagactcg aatcaactag
    45061 ctgttgagca gaaagcagga gtgcaattgt tgcaagatct tcatacgctt cggacacagc
    45121 ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa
    45181 gaatagctaa aaagttagaa agccagtcgt atttttcaac aattgaagaa atctgtgcat
    45241 ttgcacaaca gcaagcacaa tataaagaaa accaacgtac agttacttct tatcatgaag
    45301 agcaattagt gacagctgac cggctgcgtc aattagaaaa tatccagcta actgagacat
    45361 tcccagatac agaagagatc agccaacagt tagaagaaat aagacgtatg atcgaaataa
    45421 agcaagaaaa gatatacggt ttacaagaac aaaaaattag taatcaaaaa atctacgatg
    45481 aatgtctttc gatttatcac acaagccaga cgaaattaga tgagcttacg caattacagc
    45541 agctttcgca aacattcaac ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc
    45601 ttcaggttta tcttcaagaa gtgttacaag tagcaaatga tcatctccaa cgtttaacga
    45661 aaaatcgtta tcaatttgag ctagcggata ccattggcag ttatcgaggg aaaaccggat
    45721 tagaaatcaa tatctatgat gatcaggcag gtatgtctcg aagtgcgcat accctgtctg
    45781 gcggtgagag ttttattgca gcactttctt tagcattagc tcttgccgat gtgatccaga
    45841 cacaagctgg tggtgtagca atcgatgcat tgtttattga tgaaggattt gggtcattag
    45901 atgaagaagc tttggaaatg gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa
    45961 tgatcggcat cattagtcat gtaagagaat tgaaagaacg agtattacaa caaataagaa
    46021 ttgatacaga aggtagcgga caaagcaaaa tacgctatca acttggataa aaagggagat
    46081 tggtaactat gaaatggagt attccagaaa aagtcattgc acgtggacgt caatacttgc
    46141 aagaaggccg agtgttatcg gttgtccctg accaagaaga aaacgtgtgg catgcagaag
    46201 tcttgggcag tgaattgtat atggttgatt tggatggtac agcaaaagag atagattact
    46261 gccaatgtcc ctattgggag gaacataaat attgtaagca tacagttgct gtggaacttt
    46321 atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg
    46381 taaaagcaac aagtgaggga gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa
    46441 acaatcctca agcggtgcaa cctttgatta ttgaatacca agtagaaacg attgagacta
    46501 atcagtatca cccagagctt tctattttgg cactttactt gcgtatcggt tcattggcat
    46561 caccgaaaaa aacatatatc gtaaaaaaca tctatgattt tttacaagct tacacagaaa
    46621 aagaaactta cactgttaat aaacagtacc agtttcgtct agataaacgg gcgtttcaaa
    46681 aagaagatca aaaaatactc acgcatcttg caggaagtgc tcagacgcat caattattag
    46741 gtacgaacgg cttacaagtc aaaggaaaac tagataaaag atatctgctt ctcccagtag
    46801 aacaagcccg cttcctgtta gagcagatga atcagactac acgtttgcat ctgcagataa
    46861 atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa caaaccttta tcattcaaag
    46921 tggaaaaatt tggagaagat tatttgctgc aagcattgaa tgattttgat tttttcttta
    46981 tgcattatca gtggggagtt atccaaggga cattttattc tctgacgaaa tatcaacaaa
    47041 cggtttatca aacttggaaa caattgattc gccgattgga gaaaccagaa gtagtgttca
    47101 agaaaaaaga attaccgtct ttgtttaaag agattatccc tctgctgtca gagatcggcg
    47161 acgtagctat tgcccctgca gtagaagagg aagtagcaga tatccccgtc gaatttattt
    47221 tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc tatggtgaag
    47281 tcatctattc gactgacccg aagcatgagg tctcgacaga acaggctttt caggtgttac
    47341 gtgacacagt agaagagaaa cgagtacttg atctattcag aatgtatcgc taccagaaaa
    47401 atgaaactgg ttatgaacgt ccattgccaa gcggggaaga actatatgcc tttttccgca
    47461 cagaactatc tgtcttccgt cagtttggtg aagtcagact tggaagaaaa ttgcgagagt
    47521 tgtatttaga tgcgcataaa catcaaccga aaatcgaagt aaccgagacc ggttcatggc
    47581 tcgatattcg attcgatgtg acagggatag aggagcaaga aatcgatcat gtcctgcaaa
    47641 gcttgctgag aaatgatgct ttttatacat tagaaaatgg tcaggttctt tcttttgatt
    47701 cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg atacgtacag
    47761 aagaaggaac gatccatgta cctaaaaatc aaggattgat cattcaaaat caattggaaa
    47821 aaagcaatgc tacttttagt gaatcgttcc aaacgatggt acaagatctc attcatccag
    47881 aaagatacca ggcacagctt cccaaaggat tgaatgcaac gatgagagat tatcaaaagc
    47941 aaggttttcg atggttgaag atgttaggtc attatcagtt tggcggtatt ttagccgatg
    48001 aaatgggact tgggaaaaca ctacagacaa ttgccttttt actttcggaa aaagaagaac
    48061 ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt aatttacaac tggcaggcag
    48121 aggtgagaaa attcgcccct tctttaagta tccaagtaat caatgggaat aaaaaagaac
    48181 gtgaagaatt actggctaaa gacacagata tccgagtgac ttcttatgcc agcttaagac
    48241 aggatctagc taattatcag tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa
    48301 tggtcaaaaa tagcagtacg aaaactgcac aagcattgag agaattagct gtacctcagc
    48361 ggtttgcact gagtgggaca ccaatcgaga ataatttaga agaattgtgg tcattgtttg
    48421 caacgattat gccaggattt ttcccgacta aaacaaaatt cagggaactt gccgcagaag
    48481 aaattgcgca aatgattcga ccgtttattt tacggcgaga taaacaaact gtgttgaaag
    48541 acttgccgga aaaaacagag atgaatctat attctgcact tacagaagaa caaaaaactg
    48601 tttatttagc ttacttgcgt caaatgcgtg aagaaatcac atcaatggat tctgaagcat
    48661 tcaagaaaaa ccggatcggg atacttgctg gtctgacaag actgcgacaa atttgctgcg
    48721 atcctcgtct gtttattgaa gattaccaag gcggttccgg caagctcgaa caagtaaaag
    48781 atctactggt agctgccaaa gagaacaaac gaagagtttt gctgttttct caatttacag
    48841 ggatgctgac tatcttgcaa gaagagttag ccgagctagg gatctcgaca ttttatttaa
    48901 gaggcagtac gaaaccacaa gatcggttaa gtatggtaga cgcatttaat gctggcgaaa
    48961 aagatgtttt tctgatttcc ttaaaggctg ggggaactgg attgaatctc acgggagcag
    49021 atacggtcat cttgtatgac ctttggtgga atcctgccat tgaagaacaa gctgctggac
    49081 gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa
    49141 cgattgagga acgcatggat tcactccagc aagaaaaacg cgaactgttc caaaaagtga
    49201 tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga agacatccgc atgattttaa
    49261 gtgtaggaga aatcgaggag taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc
    49321 ctttctaaag aagcgattgt ttatttgatt taaaaaatag caaggaggct gggattccat
    49381 tttatcccag cttccttgct atcatttttt tatttctatt tttaaaggtt atacaaagat
    49441 tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc aaattggagt
    49501 ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct tgccagttag cactttttgc
    49561 acctagaaca tcattgtcgt aattatcgcc aacgtataag gtctcttctg gaagcatatg
    49621 aaattctttc tcagccagct gaaaaatctc tttttctggt ttttggaaac ctgttgcttg
    49681 agaaatgagc atattgcggg aaggaatcca attgttcaat tgaagttgct tcagtttttt
    49741 tgtctgatgg tcagtcggcc catttgtgat aattcctaat gggacattct tttccttcaa
    49801 gaaatcgagg gtttttttga cctcctcatg caagcagata ttatcaagtt cttcctcgta
    49861 aattttttga aataagaggc catcttcttc cgtaatatga gggtagtcta aatcttttag
    49921 tgattggctg atccgatgtg ctcgcatata ttctagtgtc caatctcctg ccattacttt
    49981 tgggaagttt tcatcactat gatggcggaa ccggatatat agtggatgca tatctgcatc
    50041 tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct gatcatacat
    50101 cgtatcatca acatcaaaca caactgattt aatcaaaatt ttacacgcct ttcggttttc
    50161 attgattttt tcacaaatcc ttgactattg taacagaaaa aattgtctaa aaaagctgat
    50221 aaataaaggt ttttcttact atatttctat ttccttgaca gaataaggtt tttatatttc
    50281 agtttttttc actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa attaatcatt
    50341 atggtagaag tcgattgatt tattttaatg caatctctgt ggaattttcg
//
LOCUS       DAJSVD010000007        50004 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSVD010000007 DAJSVD010000000
VERSION     DAJSVD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.007"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..734
                     /locus_tag="ON498_000329"
     CDS             <1..734
                     /locus_tag="ON498_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3938631.1"
                     /translation="EFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAV
                     NDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKK
                     FTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPV
                     HADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTK
                     IYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(822..1214)
                     /gene="rpsI"
                     /locus_tag="ON498_000330"
     CDS             complement(822..1214)
                     /gene="rpsI"
                     /locus_tag="ON498_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3938632.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1230..1673)
                     /gene="rplM"
                     /locus_tag="ON498_000331"
     CDS             complement(1230..1673)
                     /gene="rplM"
                     /locus_tag="ON498_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3938633.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2192..2659)
                     /locus_tag="ON498_000332"
     CDS             complement(2192..2659)
                     /locus_tag="ON498_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR3938634.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2779..4317)
                     /locus_tag="ON498_000333"
     CDS             complement(2779..4317)
                     /locus_tag="ON498_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3938635.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4397..5083
                     /locus_tag="ON498_000334"
     CDS             4397..5083
                     /locus_tag="ON498_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3938636.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5284..5811)
                     /gene="msrA"
                     /locus_tag="ON498_000335"
     CDS             complement(5284..5811)
                     /gene="msrA"
                     /locus_tag="ON498_000335"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3938637.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5895..6572)
                     /gene="pgmB"
                     /locus_tag="ON498_000336"
     CDS             complement(5895..6572)
                     /gene="pgmB"
                     /locus_tag="ON498_000336"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3938638.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6569..7699)
                     /locus_tag="ON498_000337"
     CDS             complement(6569..7699)
                     /locus_tag="ON498_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3938639.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7699..9882)
                     /locus_tag="ON498_000338"
     CDS             complement(7699..9882)
                     /locus_tag="ON498_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3938640.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9879..10904)
                     /locus_tag="ON498_000339"
     CDS             complement(9879..10904)
                     /locus_tag="ON498_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3938641.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10949..11776)
                     /locus_tag="ON498_000340"
     CDS             complement(10949..11776)
                     /locus_tag="ON498_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3938642.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11773..12840)
                     /locus_tag="ON498_000341"
     CDS             complement(11773..12840)
                     /locus_tag="ON498_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR3938643.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12866..13702)
                     /locus_tag="ON498_000342"
     CDS             complement(12866..13702)
                     /locus_tag="ON498_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3938644.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13699..14568)
                     /locus_tag="ON498_000343"
     CDS             complement(13699..14568)
                     /locus_tag="ON498_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3938645.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14638..15921)
                     /locus_tag="ON498_000344"
     CDS             complement(14638..15921)
                     /locus_tag="ON498_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3938646.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15935..17620)
                     /locus_tag="ON498_000345"
     CDS             complement(15935..17620)
                     /locus_tag="ON498_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR3938647.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17894..18919
                     /locus_tag="ON498_000346"
     CDS             17894..18919
                     /locus_tag="ON498_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3938648.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19514..23188)
                     /gene="rpoC"
                     /locus_tag="ON498_000347"
     CDS             complement(19514..23188)
                     /gene="rpoC"
                     /locus_tag="ON498_000347"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3938649.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23246..26863)
                     /gene="rpoB"
                     /locus_tag="ON498_000348"
     CDS             complement(23246..26863)
                     /gene="rpoB"
                     /locus_tag="ON498_000348"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3938650.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27591..28559
                     /locus_tag="ON498_000349"
     CDS             27591..28559
                     /locus_tag="ON498_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3938651.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28577..29482
                     /locus_tag="ON498_000350"
     CDS             28577..29482
                     /locus_tag="ON498_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938652.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29479..30311
                     /locus_tag="ON498_000351"
                     /pseudo
     CDS             29479..30311
                     /locus_tag="ON498_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            30447..31436
                     /locus_tag="ON498_000352"
     CDS             30447..31436
                     /locus_tag="ON498_000352"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR3938653.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31753..32277)
                     /locus_tag="ON498_000353"
     CDS             complement(31753..32277)
                     /locus_tag="ON498_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR3938654.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32564..34261
                     /gene="scm"
                     /locus_tag="ON498_000354"
     CDS             32564..34261
                     /gene="scm"
                     /locus_tag="ON498_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3938655.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(34416..35402)
                     /locus_tag="ON498_000355"
     CDS             complement(34416..35402)
                     /locus_tag="ON498_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3938656.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(35425..35991)
                     /locus_tag="ON498_000356"
     CDS             complement(35425..35991)
                     /locus_tag="ON498_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR3938657.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(36005..36421)
                     /locus_tag="ON498_000357"
     CDS             complement(36005..36421)
                     /locus_tag="ON498_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3938658.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(36878..38812)
                     /locus_tag="ON498_000358"
     CDS             complement(36878..38812)
                     /locus_tag="ON498_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3938659.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            39093..40049
                     /locus_tag="ON498_000359"
     CDS             39093..40049
                     /locus_tag="ON498_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR3938660.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            40139..41242
                     /locus_tag="ON498_000360"
     CDS             40139..41242
                     /locus_tag="ON498_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3938661.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            41255..42208
                     /gene="menA"
                     /locus_tag="ON498_000361"
     CDS             41255..42208
                     /gene="menA"
                     /locus_tag="ON498_000361"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3938662.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(43022..44368)
                     /gene="gor"
                     /locus_tag="ON498_000362"
     CDS             complement(43022..44368)
                     /gene="gor"
                     /locus_tag="ON498_000362"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3938663.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(44502..45755)
                     /locus_tag="ON498_000363"
     CDS             complement(44502..45755)
                     /locus_tag="ON498_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3938664.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(45761..46684)
                     /locus_tag="ON498_000364"
     CDS             complement(45761..46684)
                     /locus_tag="ON498_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3938665.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(46905..49397)
                     /locus_tag="ON498_000365"
     CDS             complement(46905..49397)
                     /locus_tag="ON498_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3938666.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(49404..49871)
                     /locus_tag="ON498_000366"
     CDS             complement(49404..49871)
                     /locus_tag="ON498_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR3938667.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        14330 a        10631 c         9231 g        15812 t
ORIGIN      
        1 tcgaatttaa tcgaactact aaaattattc aaagtatcac tgcacctaaa aaaaatgctt
       61 tagaagaaaa aagaatacaa gaaggtaagc aagcactatc atcaaaagaa cttactaatt
      121 ggatagaagc agtaaatgat gattttaata atcatttact tacttttcat gattacacac
      181 tttttatgat taccctgtat cttggtgata gaaaaagtga gacttacgct ttgcaatgga
      241 aatatattga ttttgaaaaa caaacggtcc gtcttaaaca tactttagat aaatatcaaa
      301 gaaaaaagtt tacaaaaggt cgaaaagata ccgtcattca agtaccagaa gttgtaatga
      361 cattgctaag tgaatggaaa tcggtacaag ctgatcaatt gttgaaatta aaaattaagc
      421 aaacgttaga tcagtatctc tttacttata ctaaaccatc tggagaagta aattgtcctg
      481 ttcatgccga ttacctaaac tatcgaatca attcaattaa aagaaggcat cctgacttag
      541 ttcatttatc tccacacaaa ctccgtcata cttatgcaac tatagctaga caaggtggag
      601 cagatatgaa tcaaatttca aatgctttaa cacactcaga tatttctaca acaaagatat
      661 atgtgaacac accagatata gttgataagg ctgtttttga agcttttcag agaggattga
      721 ataagtgtga ttgatatgtg ttcttaaatg tgttcttaaa aacaaaagaa gcactttcca
      781 aataattgaa aagtgccaga aacgttgata tataaaggat attaacgttt tgagaattgt
      841 gaagctttac gtgctttctt aagacctggt tttttacgtt cgaccatacg tgcgtcacga
      901 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
      961 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
     1021 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
     1081 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
     1141 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
     1201 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
     1261 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
     1321 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
     1381 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
     1441 tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg
     1501 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
     1561 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
     1621 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
     1681 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
     1741 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
     1801 tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt
     1861 tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt
     1921 ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca
     1981 taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggcttacaac
     2041 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
     2101 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
     2161 cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc gctttgccca
     2221 aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg ataaagatat
     2281 cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg taaagatctg
     2341 ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta cgatcccagc
     2401 ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta gaataaggtg
     2461 aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc aaatcatcca
     2521 tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg taccaatgtg
     2581 tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt aatacttctt
     2641 tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt aaaataatag
     2701 tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt cagttcgctt
     2761 ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca gttgagcaaa
     2821 ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat agattttctg
     2881 atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta tcaattcctg
     2941 tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca cagctcctaa
     3001 tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat cagccatcat
     3061 ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg tgagctgatt
     3121 ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt ctagaatcgc
     3181 cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc caaacgcatc
     3241 tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca gaccattggc
     3301 acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt ctcctttttc
     3361 tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa agatgccagc
     3421 accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat aacaaaacgt
     3481 ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc cgctcgttcc
     3541 tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag aaagcggccc
     3601 gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg ctgctgtttt
     3661 tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt gctctattcg
     3721 aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat caaaatatcc
     3781 agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat aaaagatata
     3841 gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc tgttttgcag
     3901 ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct gccgataaaa
     3961 ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc ggttatctgc
     4021 ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca ggccatgcat
     4081 cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt taattacttc
     4141 ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg ccatcccttg
     4201 tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg cttcatctgt
     4261 gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa cgttcatttt
     4321 ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat cttggttttg
     4381 aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg gatcattttt
     4441 atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta gatctcattg
     4501 tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct cgatcatcgt
     4561 ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt tgttggcaga
     4621 agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa gaaatttttc
     4681 gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat tttacttcat
     4741 ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt ggttgcaggc
     4801 cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg tgtgccttcg
     4861 ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt ggagtggtgg
     4921 cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg gaatatccgt
     4981 tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt tcattccttt
     5041 tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg ttgtgacaaa
     5101 cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg acttggagct
     5161 aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc ctcagtatta
     5221 gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag ctttttcggc
     5281 ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag aaccttctcg
     5341 ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat cttctgcagg
     5401 ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc gcccgctgtc
     5461 ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat tatagtagat
     5521 cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag tcggatcggt
     5581 ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat catattcgat
     5641 ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag ttggatttgg
     5701 tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct cttcaaatgg
     5761 tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca tataaaatcc
     5821 cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc ttgtgacaga
     5881 aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa cgaacgtact
     5941 tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt tatgttttca
     6001 cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc ttctgaatct
     6061 tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt gagaaaaact
     6121 tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc aaaataactt
     6181 gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc caatccgatc
     6241 ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa aacggatgtt
     6301 tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc aaaggctgat
     6361 ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg ctctagtgac
     6421 tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg aatggacagc
     6481 tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac aagaacgcca
     6541 tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa tgaacgactt
     6601 gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct tgctgtaaag
     6661 cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg ccaacatctg
     6721 ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga acgccctctg
     6781 gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca tctagacgtc
     6841 cttcgcctac gattacgagg tctgcttgct gtacccgttt atcgaactcc agtaaatcca
     6901 atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt cctgcaccca
     6961 ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat ctcatataaa
     7021 aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct ttttgtggtc
     7081 cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca gtaatgacag
     7141 tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta gcaattttac
     7201 ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa gcataaccta
     7261 gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg ccgatgatga
     7321 tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct cgcgtggata
     7381 acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt tctgccattt
     7441 caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg ccaaatgcat
     7501 tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat gaaaccgttc
     7561 cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa acttcttgaa
     7621 aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg aaagaatctg
     7681 gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt tttccagctg
     7741 gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt ttattactat
     7801 ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag gaaaagaaat
     7861 tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg ttactgtaat
     7921 acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag catgaattcc
     7981 gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt ggaataaatg
     8041 atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg ctttactcaa
     8101 cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa cgattggttg
     8161 agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct tgctgacttg
     8221 gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc caggacgttc
     8281 tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt gcggaatcag
     8341 ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac attctttcaa
     8401 aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat gcgccagata
     8461 gttcgtatat gcattattgt cgatatattc tgtgtattca tctggaccaa tgactctgtg
     8521 tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg ctgtttcatg
     8581 aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt ggacatactc
     8641 ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg aagcaacttc
     8701 ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt cctgaccagt
     8761 gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg ctttttgccg
     8821 tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt caggaaactg
     8881 atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc ctttataacc
     8941 ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg gagtcataat
     9001 ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat cgccttggat
     9061 cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct ctaaaatttc
     9121 tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt ctgtttgctg
     9181 aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt ttctgatctt
     9241 ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg ctttattgaa
     9301 tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg cactgatcaa
     9361 atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa aattggtttg
     9421 ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg gcttgatgac
     9481 cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag taatgacttc
     9541 cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc ccatttctaa
     9601 atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat gttcaccgtc
     9661 gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg attctgctcc
     9721 taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc gatagtcttc
     9781 ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac tcgcatactt
     9841 attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat aacttctcct
     9901 gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc ttgccagcca
     9961 tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc catacttagt
    10021 tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc atctacatct
    10081 tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa agccacattc
    10141 agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat cttgccgttt
    10201 tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg atcccatttc
    10261 ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc ccgaacgctt
    10321 tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat gccataatcg
    10381 atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc tggaacacca
    10441 gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc cgagaacatc aagatgattc
    10501 ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa aatctgcccg
    10561 tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc tggtggtttc
    10621 tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa gtgaaagcga
    10681 ttgggtgtac agacactcac gatatcggga gaaatctcat tcagcatttg tgtaactgat
    10741 gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg gctgactgga
    10801 tcaaccacag cggccaattc taagacatct gatctttttt gataataagc agcatgtact
    10861 ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt ctccctcctt
    10921 tgcttattta cttattgtta ttctttctct attctttctc taaatcattt aaatgacgcg
    10981 ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc acttcatttt
    11041 ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga agtagcgttt
    11101 gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga ttatctgcta
    11161 aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata tcatcttctt
    11221 caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga ttagagtcaa
    11281 tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg ttcaaaggct
    11341 ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga agtgattcgc
    11401 tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt aatcgataag
    11461 tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg cttagtgcac
    11521 gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata aagtcaccga
    11581 tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct ttgattgcct
    11641 gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat tcttcaaatc
    11701 cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat gcttgatctt
    11761 gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga tactttcttc
    11821 tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg gcacaactgg
    11881 atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac atgtgtcctc
    11941 gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg ctcgagcaaa
    12001 gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag ctggaaaagg
    12061 tttagcaaag gcaacataag aaatgatccc gccatagccg atccctctta aagaagcttg
    12121 taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt tatctgtcaa
    12181 ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat cggcaccgta
    12241 tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg tagtagcacc
    12301 tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga agattgctac
    12361 agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt gagcaggatc
    12421 gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt tataattatc
    12481 aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta actctccttt
    12541 ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca tatttcctaa
    12601 ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca agaataggcg
    12661 ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt cgacaatttc
    12721 tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct tttcgatctc
    12781 acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta attttttcat
    12841 cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact aaatcacctt
    12901 taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc accaatgaag
    12961 cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc aaaccgattg
    13021 gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa tcattccacg
    13081 aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat ggtataatga
    13141 tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct tcaatatcag
    13201 tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt gtttgaacga
    13261 ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa atgattttaa
    13321 ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag ctagcattga
    13381 ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg atcccaatca
    13441 ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa tacatgatga
    13501 atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat tctttcggaa
    13561 aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc atcagcatga
    13621 tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa aataagattt
    13681 tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca agacgaccgc
    13741 tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat cattgaacgc
    13801 aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca gtagatagac
    13861 atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg ctagtactgg
    13921 tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac cgtccatttc
    13981 agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg aaataaacgc
    14041 atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag caactggctg
    14101 gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca gatcattgac
    14161 gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa tagatggcgt
    14221 aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact tgcggttaag
    14281 caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga atgtgtaagc
    14341 aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa gataattgct
    14401 taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa aactgatctg
    14461 aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc ttggtgctaa
    14521 aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg aaacctcccc
    14581 ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa cttcttgtta
    14641 tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc cgacagttac
    14701 ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt tcgtgaaatt
    14761 cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa tctcagatgg
    14821 cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt cgactacttg
    14881 tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca tgtttttcgg
    14941 ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt ctagaccaga
    15001 agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg ttgggatagc
    15061 aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt aagttgaata
    15121 gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca catcattaga
    15181 gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc tcatcttttc
    15241 cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg acaaagcaaa
    15301 ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa ttccatattg
    15361 tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc aattttctgg
    15421 ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt gaacccagcc
    15481 gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt ttttggctcc
    15541 gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg tctgatcaat
    15601 cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta cttctggtaa
    15661 tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt ctaccggcac
    15721 ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt cattgatcac
    15781 acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct gttgtgattc
    15841 gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta gcagacaggt
    15901 agtaacaata atttttttca tttttttcca cctcttattt tattattttc agccacatat
    15961 attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt tgtccagaaa
    16021 acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg gaaaaattat
    16081 ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa gaatctgctg
    16141 agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt cgaatagata
    16201 gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc aactctttct
    16261 caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct gttttttcta
    16321 cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc ggcacgcctg
    16381 gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat tccgtcgtat
    16441 ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga ctggtcgttc
    16501 catctgggtt tttcttgtag tttactcggg taccctcttt cttcagctgt tccactaatt
    16561 caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata ccgtcatggg
    16621 aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt tttaaccatt
    16681 ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt ggtagcgaaa
    16741 actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata ttatctttgt
    16801 gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca gcgattgcac
    16861 ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt cctagttctt
    16921 tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct aagtaaaaca
    16981 aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag tctacttgat
    17041 cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg aacgttgtaa
    17101 gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt ggcaaatcaa
    17161 taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa gtactttcgg
    17221 cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc atttcttcta
    17281 tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg gagaaaccat
    17341 catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta tctgccaaat
    17401 acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca aaaaattgat
    17461 ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct gcctggcctt
    17521 tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc tcagacaaat
    17581 ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc tttctttttt
    17641 gggagcgttc caacgatttg atcatattat ggaaaacgct tactgtcaat agcatttttt
    17701 taaataattt tgatatacac aaaaatacaa ataagaaagc ttatgtatca ataattaata
    17761 gcttcttaaa aaaatatggt ctctcatgat catgcaagct attcctaaat tttcaaaatc
    17821 atagacaaaa aagaatgtct attgtactat ggaattattc taaaacgctc ccaaaaagga
    17881 ggtgcatata aaattgaaac ctactattta tgacattgcg aaaaaagcta atgtctcaaa
    17941 atcaactgtc tccagagtat taaataacca gccaaatatc tctgataaag caaaagcaag
    18001 tgtacttcaa gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa gaggactgtc
    18061 aggaaatggt tttgacgcga ttctagcatt atctcatcgt acttctcata cgacctatgg
    18121 caatcctttt ttttcagatg ttattcaatc catttctgcc gtttctgaac aaaataactt
    18181 cgacttgatt ctacaaacgg caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa
    18241 gatccaagaa aaaatgatcc gaggaattat tattttaagc tctacgatgg atgaaacact
    18301 tctttttgaa ttggatcaat acaatattcc tattgtggta atcggaaaag tcggaaacga
    18361 ctacacgaac atctactcag tggacacgga taatttccag gatagttacg atttggtgga
    18421 aacactgatc aagaatggac accgtcatat cggctgtctc catgctccga cagaagttta
    18481 tgtatccaat gatcgtctca atggttaccg tcaatgctta tttgatcacc acctagatat
    18541 ccgtaatgaa tggattatag acggcggctt tcgcttagaa gatagcatgg cagcaatgaa
    18601 tgatttaatg aagctacgag aaagaccaac tgcgattttt tctaccgatg cattaaaatc
    18661 attgagtatt tacaaagtgg cagaacaatc tggaaagcag atacctgatg atctttcgat
    18721 cgcaggattc agtgatccct tattgtcctc cctattttcg ccacagttga ctcggataga
    18781 cattccgaca aatcagttag gaatcaaagc cacagaactt ttattccaac taatccacca
    18841 ccaataccct gaagaaaaaa gaatcattct ttcaaccgag attactctca cagattcgat
    18901 ccaacaacaa gaaatgtaga acagaattat aaaagaacag aaacactgag gataagtcag
    18961 ttactctgtt cttttttcaa tcactcatcc ctcgttaccc cctcccggta ttttagcgaa
    19021 tttttcttat tttccatctg ttttagatag ctgtggataa ctataatgtt tgtgttaaac
    19081 tattttttcc acagggaaga aaaaactatt tttaaccacg ttgtgtataa gtttttatag
    19141 ttcgtttttt taaacacatt tacccttatt tcaatccatg ttatttgggg atacttatct
    19201 ctaaaacata aatagaataa cagacatgtc tactggtttt gttttattcg aggaattatt
    19261 caataatagc tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa tttattttgc
    19321 attcaaaaaa tgatctatct ttacttttgt tgattgactt ctcttggaag atgatcgttc
    19381 cgatacctta attggatacg aaccgatgtt gttaagatcc tttcccactt ttcttctagt
    19441 tagacaaatt attttagaca aaccaaaaaa gaggttgtga caataatttg tcacaacctc
    19501 ttttttaaaa tatctatttt gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt
    19561 caatatctga aatactgtag acattttcgc ttgctacacc gacttctttt ggttccatgt
    19621 cacgataacg ttgcatacca gtaccagctg ggatgatctt acctataata acattttctt
    19681 tcaaaccaag aagaggatct ttcttaccgc ggatcgcagc atccgtcaag acacgagttg
    19741 tttcttggaa tgatgcagca gataagaaac tatttgtttc taatgatgct tttgtgatac
    19801 ctagtaagac aggtcgagaa gttgcaggaa cgcctcctga gactagcgta ttgtagtttt
    19861 gatctttaaa gtctgcgatg tctaataatg tacctggtaa gatatctgta tcacctggat
    19921 ccattacacg aactttacgc agcatttgac gaaccattac ttcgatatgc ttgtcgccga
    19981 tttctacccc ttgcatacgg taaacacgtt gtacttcacg aagcaagtag ttttcaacag
    20041 ataatacatc gcgaacttgc aacaattgtt ttggatcgat tgatccttct gtcaatggtg
    20101 caccacggtg gatcatatcg ccttctgcta ctttcatacg tgctgtataa ggaacagtat
    20161 acgtacgtgt atctgttttt cctttgattg ttacttcttt ggtacgtgtt gcaggatctt
    20221 ctgagatatc gatgacatcc cctgttactt ctgtgatcac tgcttgccct ttcggattac
    20281 gtgcttcaaa gatttcttgg acacgaggaa gaccttgagt gatatcgtct ccggcaaccc
    20341 cacctgtatg gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt gattgagcgg
    20401 cgatcgtacc aactgcttct ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt
    20461 aacaatgttt gcagacacca tgttttgtat tacatgtgaa gacagaacgg attgtcactg
    20521 tttcgacacc tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat tggtttcctg
    20581 caatgatcaa ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa cgtcccacta
    20641 aacgatcttc taatgattcg atgatttcat ttccctcgcg gatggcttgg atttcaagac
    20701 ctcggtcagt accgcaatct tcttcacgga tgattacatc ttgggccacg tcaaccagac
    20761 gacgagtcaa gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc cctttacgag
    20821 ccccgtgagt tgagataaac atttccaaga cggatagtcc ttcacggaag ttcgaaatga
    20881 ttggcaattc catgattcga ccattaggag cggccatcaa accacgcatt ccggcaagct
    20941 gtgtaaagtt ggaaatgtta ccacgagctc cagaatcaga catcatgaag attggatttt
    21001 tcgcttccaa accttccatc agtttttgtt ggatgctgtc ttttgcagca ttccaaacag
    21061 caatgacacg ttcataacgt tcgtcatctg tgatcagacc acgacggaat tgttttgtaa
    21121 tagtttcaac ttgtttatgg gcattttcaa tgatttcttg tttttcattc agtacactga
    21181 tgtcagcgat ccctactgtg ataccggcat gtgtcgaatg tttgtaccct aagtctttca
    21241 tacggtcaag catcttagaa gtttctgtta ctttgaatcg tttgaagact tctgcgatga
    21301 tgtttcctaa gtttttcttc ttgaacggta atactaattc ttgctctttg atatgtgcag
    21361 gaatatcagt accagcttca acaaagtatt tgtccggtgt ttggacggtc aagttgaagt
    21421 ctgttggttc attcagataa gggaattcag gaggcatgat ctcgttgaag atgatcttac
    21481 caactgttgt gatcatcgta cgttcttttt gccattcagt aaatggtttt tcacctaatg
    21541 agttagggtt cacaccgata cgtgaatgta aatgaacata tccgttgcgc catgcgataa
    21601 cggcttcgtt ttgatctctg aagaccattc cttcgccttc gcgtccttct tcttccattg
    21661 tcaagtagta gttacctaga accatatctt gggaaggagt aacaactggt ttaccatctt
    21721 tagggttcaa gatgttttga gcagctaaca tcaacatacg tgcttctgct tgtgcttctt
    21781 cattcaatgg aacgtggaca gccatttggt ctccatcgaa atcggcatta taagcttcac
    21841 atacaagtgg atgcaaacgg attgcgcgtc cttgtactaa aactggttcg aatgcttgga
    21901 taccaagtcg gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt
    21961 cttctaaaac atcccatact tcgtcttctt gacgttcgat tttacgtttc gcatttttga
    22021 tgtttgaagc tagttcgcgt tgaacaagct cacgcatcac gaatggtttg aataattcaa
    22081 tggccatttc ttttggaagt ccacattggt acatcttcaa gaaaggtcca acaacgataa
    22141 cggaacgacc tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt
    22201 tccctttcaa catatgagaa agagatttca atgggcggtt acctggtcca gtaactggac
    22261 gtccgcgacg accgttatcg atcaatgcat ctactgcttc ttgcagcata cgtttttcat
    22321 tttgaacgat gattccaggt gcattcaagt ctaacaaacg tttcaaacgg ttattacggt
    22381 tgattacacg acggtaaaga tcattcaagt cacttgttgc aaaacgtcca ccttctaatt
    22441 gaaccattgg gcgtaaatct ggtggaataa cagggataac gtccataacc atccagcttg
    22501 gttgatttcc tgaagcgcgg aatgcttcta aaatatccaa acggcggatt gcacgtgtac
    22561 gtttttgtcc ttgtgcagat ttcaattctt ctttcaattc ggctgcttct gcatctagat
    22621 caacattatc aagtaattgt ttaatcgctt ctgcacccat agcagcttgg aattcttgtc
    22681 catattgttc acgtttttcg cgatattcgc gttctgtcaa caattgtttt ttctctaaat
    22741 tcgtatttcc tggttcgatg actacatatg aagcaaaata aatgatttct tccaaagcgc
    22801 gtgggctcat gtctaataca agacccatac gtgaaggaat ccctttaaag taccagatat
    22861 gtgaaacagg agctgctaat tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag
    22921 taacttccac tccgcatcga tcacaaacga ttcctttata acggatacgt ttgtattttc
    22981 cgcaggcaca ttcccagtct tttgtaggac caaaaatccg ctcacagaac aacccttcgc
    23041 gttcaggttt taaggtacga tagttaatcg tctcaggttt ttttacttca ccgtaagacc
    23101 agcttctgat tttttcggga gaagccaaac ctatttgcat actttcgaat ttatttacat
    23161 cgatcaaaag gggttccctc catttcattt aatggcaaac cgcaagacgg ctgatcttcg
    23221 accgccttgc agctcataga ccggattagt ctaaattatc ttctgcggtt tcaaagtttt
    23281 ggatgacatc gtctttttca tcttccactt gttcagcagc tttcttttct aattctttgg
    23341 cagtttgctg ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat
    23401 cgtccatgtc gcgaagttcg atctctgttt cttcgatatc caagacacgc atgtccagac
    23461 ctagtgattg caattctttc accaatacgc ggaatgattc cggtacacct ggttttggaa
    23521 ttggttcacc cttgacgatt gcttcgtatg ttttcacacg tccgactacg tcatctgatt
    23581 tgtatgtcaa gatttcttgt aaagtataag ccgcaccgta agcttccagt gcccatactt
    23641 ccatttcacc aaaacgttgt ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa
    23701 ccaatgagta aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt
    23761 tgatcatgta catcacaccg acagacacac gtccatcaaa tggttcacct gtacgtccgt
    23821 cgtacagaac agttttcgca tcgctggcca taccggcttc acgaactgtt tcccatacgt
    23881 cttcatcact tgcaccatca aatactggcg ttgctacgtg gatacctaac tgacgtgcag
    23941 ccatacccaa gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc acccctaaag
    24001 gattcagcat gatatcgatt ggtgttccat caggtaggaa tggcatatct tcttccggca
    24061 taatacggga tacaacccct ttatttccgt gacgaccggc cattttatcc ccttcatgga
    24121 ttttacgttt ttgaacgata taaacacgaa ctaacatgtt tacgcctggt gacaattcat
    24181 ctccagcttc acgagtaaag attttcacat cgtgaacgat accgccgccg ccatgtggta
    24241 cacgtaatga tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca tgcaatagac
    24301 gttcttctgc agataattct gttacccctt ttggcgtgac tttccctaca agcaaatcgc
    24361 catcttggac ttctgcacca atacggatga tacccatttc gtctaaatct tttaatgcgt
    24421 cttccccgac attagggatt tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg
    24481 cttctgattc atattcttca atatggatag aagtgtatac gtcatctttt accaaacgac
    24541 ggctcatgat gatcgcatct tcatagttgt acccttccca agtcatgaaa ccaactaaaa
    24601 cgttttggcc tagagccatt tcgccttgtt ccatagatgg tccgtctgcc agagtatccc
    24661 ctttttcgac tttctcaccc aaatgaacga ttggacgttg gttgtagctt gttccggagt
    24721 ttgaacgacg gaattttgtt acatcgtatt tatctaatgc accgttgtcg cggcgtacgc
    24781 ggatttcaga agcatcaacg tattcaacga caccgtcatg tttacataac aaagcagctc
    24841 ctgagtcatg cgctgatttg tattccatac cagtaccaac ccatggtgct tgaggattga
    24901 tcaacggcac tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt
    24961 tttccaagaa aggaatacat gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca
    25021 tgtagtctac tttatcaata gatacttcta agttttcact ttgagcacga gccataacaa
    25081 cgtcttgagc aaatgttcca tcttcattca atgggctgtt tgcttgggct acgatgtaat
    25141 ggtcttcgat atcagctgtc aagtagtcga tttggtcagt aacacgacct gtttcacggt
    25201 ctacacgacg ataaggtgtt tcgataaatc cgaatttatt tactttcgca taactggaca
    25261 aactattgat caacccgatg tttgggcctt caggagtttc gatcggacac atacggccat
    25321 agtgggagta gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc aatccgccag
    25381 gtcctaaggc tgaaagacga cgtttgtatg tcaattcgcc aagagggttt gtttgatcca
    25441 tgaactgtga caactgagaa gaaccaaaga attctttgat acttgccaca actggacgga
    25501 tattgatcaa ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa
    25561 ccacacgttc catacgtgct agcccgatac ggaattggtt ttgtaaaagt tcaccgactg
    25621 aacggatacg acgatttcct aagtggtcga tatcatcgac attgccgata ccttccatca
    25681 agttcaagaa atagctcatt gaagcaatga tgtcggctgg acgaaccgtt tttacagctg
    25741 cttctggata gccgtttccg atcacgttta cttcacgttc agggtctctt ggtgaaaata
    25801 ctttgatgac ttggacagtc attggatctg tgaccacacc gtcttctgat ggatagtaag
    25861 tcactgaatt taggccatta tcaatgaaag gagctagtgt ttccatgact tggtgagtca
    25921 gaactgttcc tttttcaacg atgatctcgc ctgtttctgg atcaactaat gtttcagcta
    25981 atgtcagatt caatagacgt gttttcaaat ctaatttttt gtttactttg taacgaccaa
    26041 cgtttgctaa gtcgtaacgt tttggatcga agaaacgagc attcagcaag ttacgtgagc
    26101 tgtctgctgt ttttggttcg cctggacgca aacgttcgta aacgtctttc aaaccttctt
    26161 ctgttcttga gtcgcttgcg tttttatgca agtctttttc gactgtattg cgtaatgttt
    26221 cgctgtcgcc gaagatttca aagatcgtat catcggaacc aaatcctaac gcacgaacta
    26281 atacggttaa aggaatctta cgtgtacggt cgatacgtac ataagagatg tcttttgcat
    26341 cagtttccat ttctagccaa gctccacggt tagggataac agtagaaccg aatccttctt
    26401 ttccgttttt gtcgactttt ccatggaaat agacacctgg tgaacggact aactgagaaa
    26461 cgattactcg ctctgccccg ttgatgatga atgttccttg ttctgtcatt aatgggaaat
    26521 caccgaagaa tacttcttgt gctttgattt cgccagtctc gcggtttgtc aaacgcaatg
    26581 ttacatgtaa tggtgcagag tagtttgcat catgcgcacg agcttctgct actgtatact
    26641 ttggttcttt caattcataa tctacaaatt ccaaagatag attgccattg aagtcatcga
    26701 ttggcaaaat gtcttcaaac atttctctaa gtccctcatc taagaaccat tggtaagagt
    26761 ctgtttgaat ttcgatcaaa tttggtaatt ccaacacttc actgattctt gcgaaacttc
    26821 tacgttcgcg atgttttccg tattttacta cgtgtccagc caagctcttc acccctctaa
    26881 aatgtcattc aaaaagacta cccgatcaat tgttacaata atatttcaaa tattattttt
    26941 aggtaacaac agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc
    27001 tcttgaaacc ttacttcgtg agcttctact tttgtttcct tatattaaag ccgaaacgga
    27061 caatatttcg tttctgcctt ttcttcatca gatagttttt gcaacttcat atactacact
    27121 tattttctat aaaagtcaac acttcctggc gtttcttctg ctttttcgca acattttctt
    27181 aatgttttca attttatgtt ttcaattatc tgacaattct taattgacag acctttttat
    27241 ctcggttata ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac caacatcaac
    27301 caaatcgcac caacacaact gaataacttt tactttagct actttagata ggtttgtatt
    27361 catcatgatg gatacaaacg ttaaacgaca gtttgtatct atgatggcta aagaactttg
    27421 tattgcagca aaaagccacg tagacaaaat gctaagtggc tgagcagtga aaatacaaag
    27481 taaaagtatc aagcattcta ctttttcaat ttattttccg acttttcaat ccatttggac
    27541 attttgtctg aatggatttt ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa
    27601 atataatcat cggattaggc gctgccctgc tactagcttt aggaggttgc gcacaaaaag
    27661 acaattccgc agaggcatca gatggacaaa aaacaattgg cgtattgcaa cttgtacaac
    27721 ataactcgtt agatgctgcc tataaaggat tcaaagaagg attagcagaa gaagggtata
    27781 aagaaggcga taatctaaca atcgattatc aaaatgccca aaacaatcaa gacaacctga
    27841 aaagtatgag tgagaaactc gtcaaagaag atcctgattt gctacttgga atcgccactc
    27901 cagcagcaca atctttatta aatgaaacaa ccactatccc gatgactgtc acagcggtca
    27961 cagacttgga acaagcccat ttagtcacat ccaatgaaaa acctggcggc aatgtgactg
    28021 gcacgacaga tatggtgcca atcgaaaaac aaatcggctt actgttaaat atcgtgccag
    28081 acgccaaaac aatcggcatc atgtataaca acggtgaagc aaactctaag atccaagctg
    28141 atttggcaga aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt
    28201 ccacaaatga tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat ggaatctata
    28261 ttcctactga taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa
    28321 caaaaacacc tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt ctagcaacag
    28381 taggaatcga ctacgaaagc ttgggtaaac aaacagggat catggcagca aaaatcttag
    28441 acggcaagaa tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca
    28501 ataaagacat ggccaaagca ttaggcatca atccagacag catcaaagaa gctgaataaa
    28561 acacaggagg attctcatga caaatttgct ctctcttttc ttatcatctg catcgcaagg
    28621 ggtcttatgg gccctacttg cgattggtgt atacgtcact ttccgtatct tggacattgc
    28681 cgacttgact gctgaaggaa gctttccttt aggtgcaggg atcgcggcag taagtattac
    28741 aaatggctat catccattga cagcttgtct tctaggcttt ttagggggtg cggcagcagg
    28801 gcttgtttcc ggactgcttc acacaaaatt gaaaattccc gcccttcttg cagggatcat
    28861 caccatgact ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt
    28921 attgggtcag ccaacgatct ttcattgggt cgagtcattc ggcataacaa aagacaacgc
    28981 tgtactgatc gtcggtctgc tttttgcttt gatcgttgta tgtttacttg ttttgttctt
    29041 acgtacagaa acaggacttg ctattcgtgc aactggcgat aatctggcaa tgagcgaagc
    29101 aaacgggatc aacacagata atatgaagat catcggttat atgatttcta atggatgcat
    29161 tgctctttct ggctccttgc ttgcgcaaaa caacggcttt gctgatttaa attctggtat
    29221 tggaacgatc gtaattggac ttgcttctat tatcatcgca gaagttttgt tacgtaacca
    29281 gccactattt ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc gttttatttt
    29341 agcgttagtc ttcgaactga atgttgaacc aagtgattcg aaacttgctt ctgctttcgt
    29401 gctagtgatc tgtctttctt tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa
    29461 gaaaggcggt agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca tcataccttt
    29521 gaaaaaggaa cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc cttagatccc
    29581 ggcgacttcg tgacgatcat cggtggaaat ggggctggta aatctacttt gctgaacagc
    29641 atcgccggta cattttctgt cgatcagggt caaattttat tgaatggaaa aaatatcacg
    29701 aaaaaatctg ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta
    29761 gggacagccg ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa
    29821 aaacgcggct tcttccgtgg tgtcaaacca caagaccgaa catttttcaa agaccagctg
    29881 gttcggctaa atttggggtt ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga
    29941 ggacaacgtc aagctattac gttattgatg gccacattac agcgtccaga actgatctta
    30001 ttagatgaac acactgcagc gcttgaccct gctacttcca aaacggtgat ggaattgaca
    30061 gaaaagctga tcactgagca gaatctcact gcttttatgg tcactcatga catggaagac
    30121 gcgatccgct atgggagtcg actgatccga tccgctatgg gagtcgactg atcatgctgc
    30181 atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc acagtgcccc
    30241 aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat caagtacttt
    30301 tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc ttcttttttc
    30361 tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa aactattttg
    30421 ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg acaacgaaag
    30481 acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa aagttggcac
    30541 agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg gaaaaggcag
    30601 gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac gtactagatg
    30661 caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta caaacgatgt
    30721 tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga gattctgttt
    30781 cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga cggccttttt
    30841 tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac aaacggtttg
    30901 atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc attgaatctg
    30961 taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat ggaaaaaaag
    31021 tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc agtcatatca
    31081 tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct gatctccaag
    31141 aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat cagctaatca
    31201 gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg ttggacttct
    31261 ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa ggaacgactt
    31321 atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa acaaatgaac
    31381 agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg caataattat
    31441 cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa ttctcttcaa
    31501 aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa ggtattctaa
    31561 aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa acaagagagt
    31621 catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc ttatagatag
    31681 accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt aagttctttt
    31741 ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca cctgaccaga
    31801 gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga ctggtggtgt
    31861 ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta agtaagaaat
    31921 agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat tagcttggat
    31981 ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta tcgtcatcgg
    32041 aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta aatcagcaat
    32101 ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt tgatctgcgg
    32161 ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt caaacaaacg
    32221 ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat aatccattta
    32281 ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac ctacttatat
    32341 gttactacac acccttattt tttacacttt aacagtcacc atcatcaata gtttggagat
    32401 tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata tgaacgtttc
    32461 attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac taggagaaaa
    32521 agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa aaagggcaag
    32581 tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg ccgtttcagc
    32641 ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg gtaactatgg
    32701 cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa ctgtgaatgc
    32761 acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt ttggtacaga
    32821 gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg attctttcgc
    32881 taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg ttggcagtaa
    32941 cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc ttgatcactt
    33001 aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt tcgagcagga
    33061 atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag ctaaaacata
    33121 tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg atttatcaga
    33181 taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata cgccacttca
    33241 aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt cttctgcatt
    33301 gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag aagatttttc
    33361 ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa tcagtgctcc
    33421 ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa accaagacgg
    33481 aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg atcctaacga
    33541 aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta cagatacatc
    33601 taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata ctacctctac
    33661 cacaagtgat agcacggata catcagcttc aagcgacagt acagatacta cctctaccac
    33721 aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct ctaccacaag
    33781 tgatagcacg gacacgtcag cttcaagcga cagtacggat acatctacca caacaagtga
    33841 tagcacggac acgtcagctt caagtgacag tacagatact acctctacca caagtgatag
    33901 ctcagattca tctaccacta caagtgatag cacggacacg tcagcttcaa gtgacagtac
    33961 agatacatcc tctaccacaa gtgatagctc agattcatca acttcaagcg atagtacaga
    34021 cacgtcgtct accacaagcg atacacgtca tgcaagtgtc tccttctcaa aaacaagttc
    34081 ggataacagg acgaacttta acttaactaa taaaacaagc aatgacaatg gaaacaaatc
    34141 aaaaagagca cttcctaaaa ctggaagtca atcgaataat tggataacac tcgcaggcgt
    34201 tatattactt gtaatcggtt taagaatcac ttttatgagt tataagaaat cgaaaaggta
    34261 aaaaaatatt caagataaaa aagagcaacg tcgctaacgg acgttgctct ttatttttgc
    34321 tttttatcat aaatacaaga actacaattc aatttgtcaa aaaacacgta gatttccttc
    34381 tgagaaattt atttacgtat cgaacctttc cctattcagt ctttgcgatc caaaatcagt
    34441 tgggtaaggg aaaagagatt ctcttttgtt cgaagagagg tggcaggtaa tttctttatt
    34501 tccttcaaag ccttttctgt gtaagaggcc gctaacttcc tagcttcttc cacgcctcct
    34561 gtatgttcaa cgattttgcg tacggcgtcg acatcttctt ctgtcattgc cgctttcttc
    34621 tctaaataag gtagtagctt tggtttgttt ttttgcaaag agtagatcaa cggtaaagaa
    34681 tagacccctt gacgcatgtc ttctagtaca ggtttgccga tttcctggct ctcttttgta
    34741 taatctaaaa tatcatcgat aatctgaaag gccatcccga tagaaagtcc gatatctcct
    34801 gctttttttg cgaagcgctc gcttgttcca ctttcaaaag cgccaacaga acaacttaat
    34861 tggaacagtt cagctgtctt acctgaaata ttttccaaat attcttttac agaaagttca
    34921 taattatagc gatcatccat ctgccctaat tcgccattca atactttttc catactgcga
    34981 gaattcaatt ggagactttt caaggaagtg gagtaatcag acaatagttt aaaacagcag
    35041 acaaataaat agtctcctgc atagacagaa acgacattac caaactgact acggatatta
    35101 ggctgaccac gtcgcagatc cgcttcatca acgatatcat catggatcag tgtggccgta
    35161 tggagcattt cgatagaagc tgccaaagcg atggctttgt ctcgatcttg ctctggtcca
    35221 aattgtgaga acaatagctg ataggcagga cgcaacagct ttccgccggc atggatcgtt
    35281 tctttgattg cttgttcgac aggtttgttt ttcaggcgga cagtttcttc catccgtttt
    35341 agtgtttctg tcaattcttt acttaaagca ggatagtttt cccacattgg atgtagcttc
    35401 ataaaaaaac tccttgtgtg acgattattt tttggtaaac attgggctcc atttttgttt
    35461 ggttccctgc tcataatctt tttggaatcg ttgaagtgtc tgcacatgtc gtaaaagata
    35521 attcgctgcg atcccgattg caatccctga taaaatccct aaaactgaaa ggactggcaa
    35581 gtacaacatg actgtccatg attgtgcaat gaaagaagcc atcaaaagct gtccgacatt
    35641 atgcatgaaa ccgccggccg cactaattcc gatgatactg acacgttttg gtcctaattg
    35701 cttgaccaat aacattccaa aataactaag caaagcacca ctcatactat aaaggaatgt
    35761 ggaaatcgtt ccgcctagca aagtagtcag gaatagtcgt agccaaagta gtaagaagct
    35821 gtctcgtttt ttcatggtaa aaatggcaac gatcgtgatt agattggcta agcctagttt
    35881 tgcaccaggc gcaaaagaaa aaggataagg gatcatattt tccaataaac caatgatcac
    35941 tccttgtgca acaagtagcg aaatataaat cattttttgt aatttactca tggtgtgtga
    36001 aggtctaata aatcagactg tcttcgtcac ctccatctgt tgaacggact tcgatcacta
    36061 atttatgtgg caaacagacg atggtttcgc catttttcgt tgcccagcct cgacgtacgc
    36121 agatctggtc atcgcaatct gcttctttta tccgtactcg atcgccatct atctcgatca
    36181 agttataatc tccgtgttca tctttgtaag tatatgtgtt gctctcttct ccagccatca
    36241 aagggaatgt tttgatttct gtgccatcca cacgcagtac cgcttctttt tctgctgttg
    36301 cggtgttttg ctgaagacca aaaatcatga ctggtaaaaa agaaagaaca gtcaaaagca
    36361 aaataataaa aacatcccaa ggtttgagca gacttttttc agcaaattct ttaaggctca
    36421 tttttgttct cctttttcca gattaactca cccaatttta gcataaaaac aagaggttgt
    36481 gacacctgtg tcttgctttg agtaataaga acgaagaata gaaaacagct ttccatgttt
    36541 tctaattctt tgggcttatt accgagaagc agcaggtgga acaccgttta taacaagagg
    36601 ttgtgacaga agtgggcaac ttcaagaaat aagaaggaat ttacgaaaat tacttttcaa
    36661 gtttttgtga aatttcggct tatttttcga agaagctact tctggaacac cgtttataac
    36721 aagaggttgt aacagaggtt gtgacacctg tgtcttgctc atggaggctt atctcgggca
    36781 tcttattcct ttaattttac ataaaaagga ggctgtgacg gtttgtctca gcccctttct
    36841 attgaaatct tgcattcaaa taacgttctt tatctattta tttcttgccg tactgggaac
    36901 ttggtctacc gtaccaccaa ttacctagtg tacgttgtag ccatggaatt acccagcgat
    36961 ccaacccaat cgcacgtccg gaaccgttca tcaatgcgaa agcaacaaaa atgaaccaaa
    37021 tgtttaccca gtaaaacatg cctgataagc agaacgtgat caccaataca atcgttgtcg
    37081 cactgcatag ccatgtgaat aatccagcaa tcaatgctaa tgccaaggca atttcaaaga
    37141 ataccatgaa tttttggaag aacattgcta catcattgtt tggcatgatc acttgcatga
    37201 tctttgtgaa ccagtctggt gacttctcaa tcacagccat tggttcttca ccgtatgcat
    37261 aactcaaacc aaaatgtgca gcttgagtcg ctgcttctcc ggcacttgcc cctgctccgc
    37321 tggcagctgt ggttgcatca ccagcagcag aagcaccact ggcaacatct gttacattgt
    37381 attcccatgt ccaagggaac acactgtttc cagtgaacca tgatccttca ccaaaccaag
    37441 tacttggttt gaacacttca ccattcccaa tgattttttt ggaagcttca acgaaccaaa
    37501 ctaaaccata gaaaatacga agtggcacac tccataagac attgccgtaa cgagaagaat
    37561 gtcctcgagt cacgttgcga tcatctttga tatggaaaaa ctcatgcatt aaatattgga
    37621 acatataata accagaacga atatcaaaga agtatttcaa attcacgata tgtttcatta
    37681 cgatagctaa aaatccgctt aaatggatct tatcaaatag gttagctacc ccccatttgg
    37741 caccaataga aaccataaat ccttgatagt ttcctttaaa tttatgcttt tctccgcctt
    37801 tgatcgaagc cacgatattc gctgcggctg tgtgtcctgt ttgttcagca gcttgaacga
    37861 tctgcggtgt cggtgttcct gggaattctt cataaaagac taaatcacca atgatataga
    37921 tgtttttatc ttcgtagcct tttgcctgca tatattcatt ggcgatcaac cgctgtccgc
    37981 gtgctgattc taatccaaag tcagcagcgt cagaagttcc tttgacacca gctgtccaaa
    38041 tcagtgtgtg agtcggaatg ttgcgtccgt ctttcaattt gatatgatca gcagccactt
    38101 ctacgattgg tgcattcaat aataattcca cgttgtgctt acgcaaatag cgttcagctt
    38161 ttgcagcatc atttcgatcc aacatattca agatcgtcgg cattgcctct acgaccatca
    38221 gtgtgatttc gtttggatct aatttataat ctttggctag gcgatttttc cagtcgatca
    38281 gttccccaat catttcaata ccggtaaatc cagaaccaca gacaacgaaa gtcagcatgg
    38341 ctttacgcag ttcatcatct ggttccaatg cagcttttgc tactgtcttc tcaatatgtt
    38401 cccgaatcct tacagaatct tcaaatgacc aaagggtgaa tccgtgtgct ttgacaccag
    38461 gtgtaccaaa gtcattcggc tctccgccca ttccaaggat cagctgatca aaaggatagg
    38521 aacctaattt ggtcttgacg atttttcgtt ctttatcgat accgattaca gtgtctgtga
    38581 caagttgtac attcttttga cgagcaaaca aatgctgcag atcgtattgg attgcttctg
    38641 gttctacacg tccgccagct acttcatgca actcggtcat catagtatgg tacgaatgac
    38701 gatcgatcag agtaattgct acatcttctt ttttcagttt ctttgccaag tattttgtag
    38761 ctgaaacacc ggcatatcct gctcctacaa cgacaatatt ttgctttgcc attgatcatc
    38821 cgctccttat agactatcta tataatgaat atatgataat tcttttagtt tgatacttat
    38881 tacacaatct ccatatatta tatagatgtt ctgttctttt gtctaactcc ttttggattt
    38941 ttttcaaatt ttaaaataaa gcgttttttc acgaaatttg ctgaaagaaa acgtttgaaa
    39001 tattacatgg gttatttatc aaaaatagtt gacttatagg ctaaaaagat tagaatgtat
    39061 aatgaatcac aaagaaaaat tgtggactag aaatggaatg gtggggtatt aaaatgaaga
    39121 aaaaacgttt tgtaactgga tttgccgtat tagcattttc agctctagtg ttaggcgctt
    39181 gcggcgcaga tgacaacgga agctcaaact cttcttctga atcatctaca gcacaatctt
    39241 cgactgcaaa aagcacaaca gaatcatctg caaaagttgt agctggcgga gatttgcaag
    39301 acggcacata taaattagaa gaaaaaaatt attcaaatgg ttaccgtgca gtctttgaaa
    39361 tggttgtaaa agacggcaaa atcactgaat caaaatatga taacgtcaat gaaaacggcg
    39421 aatcaaaaac aaaagatgca gaatacaaca aaaacatgga agcaaaatct ggcacaaacc
    39481 cagaaaaata cattcctgag ttgaatgacc aattcttaaa agcacaaagc gcaagcggtg
    39541 tggaagtcgt aactggagct actcactcat ctgaaagctt ccaaaactac gcacaacaat
    39601 tgatccaagc tgctcaagct ggtaacacag atacaatcga aatcgacaat ggtgctgatt
    39661 tgaaagacgg cacttacaag cttgaagaaa agaactactc taacggctac cgtgtgcaat
    39721 tcgaaatgac agttgctggc ggcaaagtaa ctgaatcaaa ctttgattat atcgacaaag
    39781 acggtaaatc aaaacaagat gatacagaat ataatgaaaa tatgaaagca aaaagcggta
    39841 cagaaccaaa aacttacatc cctactctga acgacgaatt cgtcaaagca atgggcgaag
    39901 aagatggctc acctgctaat gtagaagttg ttacaggtgc tacacacagc tcacattcat
    39961 tcatcatgta cgcacaacaa ttagtgaatg cagctgaaaa aggcgacact caaacaatcg
    40021 aagtagataa tatcgtaaca gaaaaataat cacataatca tactaataaa agttggaggg
    40081 tgggacgata gccagattag gcagtcgtct cacctttcaa aatgaagaga ggaaaaagat
    40141 gcggaaaaaa ctactatttt ccttaatcac actgggcatc tcttttatgg tgctaactgg
    40201 ctgcagcaac tcagcaaata aaacaaccac tgaaagttca gcagctacca agatattgaa
    40261 agatccatat tctgatgaac agttcttgtt agggacttac gttcgtatcc gtgtgtatga
    40321 tgaaggcaaa gaatcagcat tgaaacctgc cttcgatcga gtaaaagaac ttggtgataa
    40381 aatcacgatc aatcaaaaag gttctgagat cgatgaagtg aatgagcaag ctgggatcaa
    40441 accagtaaaa gtttctgatg acgtctacac gttagtcaag cgggcctacg aatatagcca
    40501 agattctcaa ggcggattcg atatggcgat tggtgcaatc acacaattat ggcgaatcgg
    40561 ttttgatgac gccagaaaac cttcccaaga agaaatcgat caggcattga aattagtgga
    40621 ttatcataag atcgaactaa atgacaaaga aaaaactgtt tatctaaaag aaaaaggaat
    40681 gatcatcgat ttaggtgcaa tcgccaaagg ttacattacg gacgaagtag tgaaagtctt
    40741 gaaaaaacaa ggagtgacca ctgccatcgt tgacttgggc ggtaatgtct atgtcttagg
    40801 ccacagccca agaggcgaaa accaagactg gacagtcggc atccaagatc cgaacatggc
    40861 tcgcggatct gttttaggtt ctattaaaga acggaacaaa acattagtta cctctggaat
    40921 ttacgagcgt tatttagaag tagatggcaa aaaataccat catttgtttg atccaaaaac
    40981 gggttatccg ttcgacaatg atatcgcaag tgtcacgatc ataacagata aatcaattga
    41041 tggtgacggg ttgtctacag cagtcttttc aatgggtgtg aaaaaaggac tggaatacgt
    41101 cgaaagcgaa ttgaataacg ggacggaagc gatcttcgtg acaaaagacg ataaagtata
    41161 tgtgaccgat ccaatcaaag acaccttcaa attatcggag gactcccatt acacgatggg
    41221 tgaccgttct gaactaaaat aaggagtacg aaccatgaca ctaaaaatct ttttagaagt
    41281 agttgaaata cgaacaaagg tggctagtgt atttcctttt gcggtagggg tattattctc
    41341 tatcgctttt tttcatgaga tacattggct aaacactttt ttatttttta ttggaatgct
    41401 agtttttgat ttagcgacta ccgctatcaa taattacatg gattatgaaa aggctcattc
    41461 tgaagtatat aagtatgaag aaaatgtcat tggccgcaga aatctatctc ctgtattgat
    41521 tcgaaacatg attttagcta tgattgcatt tgtttttgtt atcggcgtta ttctaacgat
    41581 caatacgggt tggcttttac ttttgatggg atttatctgt tgttttattg gagtattcta
    41641 tacattcgga ccgattcctt tatctcgaat gcctttaggg gaaattttca gtggggtgac
    41701 tatgggcctt ggaatttttg ccatggtcgt ttatgtcaat acattcggtt cagatgtcta
    41761 tgatctgaca gtcaactttt catctggaca atttgctttg gtaggaaatc tttgggctat
    41821 agcagcaatc gtattagctt ccttaccctt ggtgtttacg attgccaata tcatgttggc
    41881 taataatttg cgggatcttg accgtgatat tgaaaatcat cgctatacac tagtttttta
    41941 tatcggacgt cctgcgggaa cagtcttatt ccagctattg atgtatgctt gctatttcgt
    42001 gattttaatg gggttgttga gcggtatcta tgaatggccg atcttggtca ccttcttgac
    42061 cttgccaatt gtgaagaagc atttggatca attcaagcaa acgttacccc agcctatcag
    42121 tttcagctat tccatcaaaa atatggtctt attcaatgcc agttatgtat tggggctatt
    42181 actaacaatc attatgcaag ccatttaaca gcatgaagag gttgtgaaaa aactgccctg
    42241 catcaagtaa taagaacagc caaataaaaa tagctcctta tatttttcgt ttggttgggc
    42301 ttattaccgc agatgcaagc ttttgaacac cgtttatcag gaggttgtga aaaagtcgct
    42361 tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt
    42421 ggcttaatac cgagaaactg gcttttgaac accgtttatc agaggttgta aaatcagtgt
    42481 tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    42541 gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt gactaactat
    42601 gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca gcgaacttga
    42661 cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg tttgcttctt
    42721 ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata catttctatt
    42781 tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat ggattcattt
    42841 actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat ttcgttttga
    42901 gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa aatcagtata
    42961 ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg ttaatattaa
    43021 attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact gtattgtcaa
    43081 agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc atctcatcga
    43141 caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca cagatcaatt
    43201 tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta aagcgtgaac
    43261 gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct gtcaaaccaa
    43321 ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag tcaagataag
    43381 aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg attgcaacag
    43441 gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca tccgttgtat
    43501 tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt gtattttcca
    43561 gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa tcaccagtta
    43621 aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta atttctttag
    43681 caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc aaattgtccg
    43741 aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac atgacttcac
    43801 tacctaaacc acaaaataca cctgaaagtt cagccgcaat gtatccagca cctaaaacga
    43861 ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt gcgtattctc
    43921 caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt ttaggagcac
    43981 gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg taacctttta
    44041 tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga aaatcaatat
    44101 atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct gcttggatac
    44161 cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc cacataactt
    44221 ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct tcaatcagca
    44281 aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca gagccgccac
    44341 caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag atcaatcaaa
    44401 acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc ttgaattttg
    44461 tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg ttttcggatc
    44521 atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat tggatttggt
    44581 gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg actgaaatga
    44641 ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt tctctgacgt
    44701 atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa tttatatact
    44761 gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt gtagtaaaaa
    44821 cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc ttgcttgcag
    44881 acatagtttt caggatcttc catataactg tctacagccg ctttatagac atttgacacc
    44941 gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc tacgccgttt
    45001 tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat cgagaattct
    45061 tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg catatcgaac
    45121 aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc ccgcatcgac
    45181 atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc atggataaat
    45241 gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat tgaaacttca
    45301 cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt ctcaaaatta
    45361 gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt cgcacaaatt
    45421 tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg caaactgcgg
    45481 aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc agcaacgtat
    45541 accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc ataagtaaaa
    45601 ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc atctgctcca
    45661 taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat ttctggtcgt
    45721 ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata gcagaaggat
    45781 caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta ctgatttcgc
    45841 tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt ttttttgctt
    45901 cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat ccgtccaatt
    45961 tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga ttcagatcat
    46021 tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt gtctcttctt
    46081 ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct tgtttcgtat
    46141 agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta gcaccataca
    46201 caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct tcaaaaggaa
    46261 cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg atttgacgtg
    46321 cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca ggattttttt
    46381 tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt atttctttta
    46441 aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc ccatttacag
    46501 caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc tgttcttctc
    46561 gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat agtgtatctg
    46621 cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt atgagttcga
    46681 tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa ctttccaata
    46741 cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa aataaagttc
    46801 gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac aacctcttaa
    46861 tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag ggaacctact
    46921 gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc acctactgtt
    46981 acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg atcttcaatt
    47041 tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa tccagctttc
    47101 ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc tttcaatcgt
    47161 ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt actcaatgaa
    47221 tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc tttctttaac
    47281 tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg gacattgaat
    47341 ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat aatcatgatt
    47401 gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc atccaatact
    47461 tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa gagaatcact
    47521 gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata ccctacgtat
    47581 cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc tgacatatca
    47641 acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt ggccaattct
    47701 gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg atcaggatct
    47761 ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc ctcatcttga
    47821 ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct ttcttttttc
    47881 tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac atcttcttct
    47941 gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac tttttgcagc
    48001 tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa gtcttgtttt
    48061 tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat cacagttgat
    48121 tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc gatcaaatca
    48181 attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt gactgctgct
    48241 cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt acgtaatcct
    48301 tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg agcaaaacgt
    48361 cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt cgttgctccg
    48421 atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc atcaattgct
    48481 ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa gattacttga
    48541 ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc accgcgatat
    48601 ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc ttgcatgtct
    48661 tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc tgttttgcct
    48721 acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag aatctggata
    48781 agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc ttttgctaat
    48841 ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt ttgccgttta
    48901 tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc accagctagc
    48961 cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa gttgatcatg
    49021 attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg ttctgttcca
    49081 actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac ttgtttcatt
    49141 cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc ataaccaatc
    49201 agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg taatactttt
    49261 ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga actaactgat
    49321 tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg ggctctttca
    49381 gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg ttccagaaat
    49441 gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa tgtgtttttt
    49501 gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc ttcataaagt
    49561 ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa ctgatttatc
    49621 tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat ataaccacca
    49681 ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt attaatcaca
    49741 tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct tcgaatctcg
    49801 atcatttcac tattttccag aattttcttt atatatgatt caattaaatc cgaggtattc
    49861 tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata acgaatttga
    49921 taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca acaaaaaaaa
    49981 atacggaata tactattccg tatc
//
LOCUS       DAJSVD010000008        49880 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSVD010000008 DAJSVD010000000
VERSION     DAJSVD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.008"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            62..1120
                     /locus_tag="ON498_000367"
     CDS             62..1120
                     /locus_tag="ON498_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR3938668.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1124..2158
                     /locus_tag="ON498_000368"
     CDS             1124..2158
                     /locus_tag="ON498_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR3938669.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2260..2976)
                     /locus_tag="ON498_000369"
     CDS             complement(2260..2976)
                     /locus_tag="ON498_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3938670.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2945..3730)
                     /locus_tag="ON498_000370"
     CDS             complement(2945..3730)
                     /locus_tag="ON498_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR3938671.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3721..4470)
                     /locus_tag="ON498_000371"
     CDS             complement(3721..4470)
                     /locus_tag="ON498_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3938672.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4768..5292
                     /locus_tag="ON498_000372"
     CDS             4768..5292
                     /locus_tag="ON498_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3938673.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5308..6807
                     /locus_tag="ON498_000373"
     CDS             5308..6807
                     /locus_tag="ON498_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3938674.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6895..7497)
                     /locus_tag="ON498_000374"
     CDS             complement(6895..7497)
                     /locus_tag="ON498_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3938675.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7601..8557
                     /locus_tag="ON498_000375"
     CDS             7601..8557
                     /locus_tag="ON498_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR3938676.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8862..9818)
                     /locus_tag="ON498_000376"
     CDS             complement(8862..9818)
                     /locus_tag="ON498_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3938677.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9992..10552
                     /locus_tag="ON498_000377"
     CDS             9992..10552
                     /locus_tag="ON498_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938678.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10545..11369
                     /locus_tag="ON498_000378"
     CDS             10545..11369
                     /locus_tag="ON498_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR3938679.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11379..11996
                     /locus_tag="ON498_000379"
     CDS             11379..11996
                     /locus_tag="ON498_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3938680.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12014..12697
                     /locus_tag="ON498_000380"
     CDS             12014..12697
                     /locus_tag="ON498_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3938681.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13380..14663
                     /locus_tag="ON498_000381"
     CDS             13380..14663
                     /locus_tag="ON498_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3938682.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14679..15566
                     /locus_tag="ON498_000382"
     CDS             14679..15566
                     /locus_tag="ON498_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3938683.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15583..16419
                     /locus_tag="ON498_000383"
     CDS             15583..16419
                     /locus_tag="ON498_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3938684.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16422..17045
                     /locus_tag="ON498_000384"
     CDS             16422..17045
                     /locus_tag="ON498_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938685.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17042..18334
                     /locus_tag="ON498_000385"
     CDS             17042..18334
                     /locus_tag="ON498_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3938686.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18693..18790
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18906..19487
                     /locus_tag="ON498_000386"
     CDS             18906..19487
                     /locus_tag="ON498_000386"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3938687.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19490..20797
                     /locus_tag="ON498_000387"
     CDS             19490..20797
                     /locus_tag="ON498_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR3938688.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21213..21701
                     /gene="purE"
                     /locus_tag="ON498_000388"
     CDS             21213..21701
                     /gene="purE"
                     /locus_tag="ON498_000388"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3938689.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21694..22818
                     /gene="purK"
                     /locus_tag="ON498_000389"
     CDS             21694..22818
                     /gene="purK"
                     /locus_tag="ON498_000389"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3938690.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22831..24126
                     /gene="purB"
                     /locus_tag="ON498_000390"
     CDS             22831..24126
                     /gene="purB"
                     /locus_tag="ON498_000390"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3938691.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24306..24683)
                     /locus_tag="ON498_000391"
     CDS             complement(24306..24683)
                     /locus_tag="ON498_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938692.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24939..25667
                     /locus_tag="ON498_000392"
     CDS             24939..25667
                     /locus_tag="ON498_000392"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3938693.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25667..25921
                     /gene="purS"
                     /locus_tag="ON498_000393"
     CDS             25667..25921
                     /gene="purS"
                     /locus_tag="ON498_000393"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR3938694.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25923..26594
                     /gene="purQ"
                     /locus_tag="ON498_000394"
     CDS             25923..26594
                     /gene="purQ"
                     /locus_tag="ON498_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR3938695.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26595..28817
                     /gene="purL"
                     /locus_tag="ON498_000395"
     CDS             26595..28817
                     /gene="purL"
                     /locus_tag="ON498_000395"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR3938696.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28802..30241
                     /locus_tag="ON498_000396"
     CDS             28802..30241
                     /locus_tag="ON498_000396"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3938697.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30264..31316
                     /locus_tag="ON498_000397"
     CDS             30264..31316
                     /locus_tag="ON498_000397"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3938698.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31313..31891
                     /gene="purN"
                     /locus_tag="ON498_000398"
     CDS             31313..31891
                     /gene="purN"
                     /locus_tag="ON498_000398"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3938699.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31888..33423
                     /gene="purH"
                     /locus_tag="ON498_000399"
     CDS             31888..33423
                     /gene="purH"
                     /locus_tag="ON498_000399"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3938700.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33439..34713
                     /gene="purD"
                     /locus_tag="ON498_000400"
     CDS             33439..34713
                     /gene="purD"
                     /locus_tag="ON498_000400"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3938701.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34835..35749
                     /locus_tag="ON498_000401"
     CDS             34835..35749
                     /locus_tag="ON498_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3938702.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35882..37447)
                     /locus_tag="ON498_000402"
     CDS             complement(35882..37447)
                     /locus_tag="ON498_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3938703.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37440..38891)
                     /locus_tag="ON498_000403"
     CDS             complement(37440..38891)
                     /locus_tag="ON498_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3938704.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38905..39921)
                     /locus_tag="ON498_000404"
     CDS             complement(38905..39921)
                     /locus_tag="ON498_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3938705.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40141..41229
                     /locus_tag="ON498_000405"
     CDS             40141..41229
                     /locus_tag="ON498_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR3938706.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41283..42809
                     /locus_tag="ON498_000406"
     CDS             41283..42809
                     /locus_tag="ON498_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3938707.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42811..44016
                     /locus_tag="ON498_000407"
     CDS             42811..44016
                     /locus_tag="ON498_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3938708.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44273..45250
                     /gene="guaC"
                     /locus_tag="ON498_000408"
     CDS             44273..45250
                     /gene="guaC"
                     /locus_tag="ON498_000408"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3938709.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45514..46938
                     /locus_tag="ON498_000409"
     CDS             45514..46938
                     /locus_tag="ON498_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3938710.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47399..48508
                     /gene="pdhA"
                     /locus_tag="ON498_000410"
     CDS             47399..48508
                     /gene="pdhA"
                     /locus_tag="ON498_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR3938711.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48511..49488
                     /locus_tag="ON498_000411"
     CDS             48511..49488
                     /locus_tag="ON498_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR3938712.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49513..49761
                     /locus_tag="ON498_000412"
                     /pseudo
     CDS             49513..49761
                     /locus_tag="ON498_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
BASE COUNT        15908 a         8596 c        10999 g        14377 t
ORIGIN      
        1 gtattagcaa aataaaagct ttttttattg tgaataaatg aacataatta ggaggagaaa
       61 gatgaactta tttgacattt ttcgatcgac tttgacagat atgtcgattg tcagtgcaat
      121 cacttcaaca gtttttatta tattattagg gtttgtttgt agaaaaaagg ggattttttc
      181 tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca gtggctttgc cagcactggc
      241 atttcgagct ttcatgcaag atatcaatac agaattactg atgcaaggaa tcaatgtctt
      301 gatttgggga ttagttattt atattattct tattctagtt tcaaaacctt ttttcttaag
      361 ctataaaggg gataagcaaa caacgttgcg tgtcctgacg atctttggtt ctacgacctt
      421 tttcggaacg cctatcgtca gtgcgattta tggtcctgtc ggcgtgatgt attcctcaat
      481 tttcaatatt ggctaccgaa tctttttgta ttcttatgga tacataaaaa tgagtggctt
      541 gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat ccaatcatta tcgctacttt
      601 tgccggcctt ttcatttggc tgttccaagg gtatctgcca caagtgtcag ttacaggtgc
      661 agaaggagag ataagccaag tagcgttttt gcgaattgac cagactgcac cgtggctatt
      721 tcagccgatg gattatttag ctggtttaag ttcaccattg gcgtggctag caatcggcgc
      781 aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa aaaacttcat ggtattacag
      841 tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg attctagcag ttttgacggc
      901 aacgaatatt ctaccagtca attttgaagc actggcaaca atcgttatca tgatggctac
      961 accagctgca acagtggcag cagcctatgc tatcagtttt gatagggagg cacttttagc
     1021 ctccaatgca tccttactgt caactatttt tgctgtggta ctgacacctg tatggatcgt
     1081 tgtattacaa atgattcatg gcataggttt attttcataa gaaatggagg aagaaagtat
     1141 gagaatcgca tgttacggtg ttcgtccgaa cgagatagat ttttttgaaa aattgaatat
     1201 ttaccattat gatctcagtc tttatgaaga actgttgaca catgacaata tcgtgtcagc
     1261 aaaggaccat gatgctgtat tgttgagggg aaattgtata gcagatgaga ccaatcttgc
     1321 aaaaatgcag gaatatggga ttcgttatgt ttttacacgg actgtgggag tcaatcatat
     1381 cgatctacaa gcggcagctg attttggtat gacagtagcc cgtgtaccat cgtattcgcc
     1441 aaatgcgata gcagagttat ctttgacttt tgccatgatg cttttgagaa atactgcgta
     1501 tacgacgatc cgaacatctt tcaaagattt tcgagtggat gagcaaatgt ttagtcgtga
     1561 aatcagaaat tgcacagtag ggatcatagg aacaggcagg atagggttga cggaagctgt
     1621 tttgtttaag ggactaggag ccaaagtgat cggctacgat attcatcaga cagaagctgc
     1681 taaaaaagta ttgacgttcc actcgcttga agagttatta gccaaaagcg atattgtcag
     1741 tattcatgta cctcatatcc caggtgaaaa cgatcaaatg atcaatgaag gatttttagc
     1801 tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt ggagagcttc aagataatga
     1861 agctatttta agagctttga aaagcaatca tttagctggt tttgcaaccg atgtttttgc
     1921 aaatgaaaca gaggtttttt tccgatcctt caagccatgg gagacgattc cagaccctgc
     1981 tatccagcaa ttagtagaaa tgtaccctag agttcttgta accccgcatg taggttcaaa
     2041 tacagatgag gcattatcaa atatgatttc gacaagtttc gagaactttc gtgaaataat
     2101 agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa gcccgtcaat taagataaga
     2161 aaacatggat attcgtcaat aaaatgttta aaaatagtcc aagtaaacag ttgttacttc
     2221 tgtatacttg gactattttg tatgggataa ttcgatgact caacagtaat ctgtgagctt
     2281 tttccgccca ttctttgtca attcataaca ggttagcggc cggccgattt ccaaatattt
     2341 gattctttcg ttcaatatat cttgttctac taaaaaattc aaatatttct tgacggatat
     2401 acgtgacagt gagctttttt gagccagttc ttttgttgaa aaaatagtat cttcttttag
     2461 aataaatgca cttatttttt ttaaagtcag ttttgacaga cctttaggca aatcgatgaa
     2521 tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact tgatcgatca atttctgatc
     2581 ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga tacttttcga tcgccagctg
     2641 aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag gcaccgtagc ggatcacatt
     2701 ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc actgggatat catttccttg
     2761 ctgtctaagc caagataaaa aatggatacc actttcgcca ggcatgtata catcaagcag
     2821 cagcagatct gcgttttttt gtctgaaata tttttttcct tcttctacat ttctgaaact
     2881 ttcgattacg ttcatattag ggatgctttc cacaaaagta cgattcaatt ctgcaaccat
     2941 tggatcatct tcgattatca ggacatttgt catcattttg atcttccttt tttggtaatt
     3001 ctagatagac gattgtaccg ccatttgttt gtttggaaaa ttctaaaaat ccaccgtggc
     3061 gtttgacgat tgaatggatg agattcaatc caaacccacg attgattccc ttcgtcgaaa
     3121 aacctagcgt aaatattttt tctgagattt cttctgatat cccgacaccg tcatcgcaga
     3181 cggtcacgat gaatacttca gcttctgtgt catagtttaa atgaatccgg acctgtccgt
     3241 ttctttcatg tttttgtagc acggcttctt ttgcattatc aagtaagttc cctaacactt
     3301 gcaaaagtga atggatggag tcatccatct ccattttagg gataaaagaa tcgctttcta
     3361 acagcatttc aacagattgt tctttggctt cgtttatttt gcctaataaa aaaccggcaa
     3421 ttgcaggtac tttgatcaga cttgagactt ggccaatctc ttttttataa ttttgattta
     3481 gctgctggat aaaatgggtg acttcttcat acttcttcaa atcaatcaaa ccagaaatga
     3541 catgaagttt attcatgaat tcatgggttt gagctctcat ggagtttata tattgtttcg
     3601 tccccccaag ctcctccatt aatttcatta cttctgatcg atttcgtaaa gttgcgactg
     3661 ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct agaaatagtt tctatcccat
     3721 ttaaatacat ggattgagtc cgaacggtcg tttgatgctc aatcgtctct ttaaacaaaa
     3781 cgtcatatag attatcactg atttttttcc tacttcgaaa tccaatagct gctctgtttg
     3841 gtagaaaatg ttttttgcag gtgtattgat caagaggata gttttatcag aagagatggc
     3901 taagattcct tcatctactt cattttcgat aatcgttttt tcacttaact gttctgctat
     3961 ttcttgtggt tcaagtccaa gcagtacttt tttcattttt tgggcaagaa aaacggcgcc
     4021 tatgatgcct acgctaagtc caataatcaa tgaaaaaaca atcatttttt gtccatcttt
     4081 gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa actgcaccaa taatctcttg
     4141 atcataataa atagggacga aatagcggaa gccgaaccct aacattcctt ctttttcaga
     4201 gaaaaattct tgtcctccaa tcactttttt cacatcttca tgacttgaga aaggaagacc
     4261 gatgtaactt gggtctgcgt gagaatatcg aatgccttca gggtccatga caacgataaa
     4321 atctaaattc gatgcaatca tgatttcatt tgttattcct tgaatcgcat gtgtcgggtc
     4381 accagtctgc agcttttttt ggatgagttt atcattggca acgattttag caatgctaga
     4441 gattttatct gacacactag atcggttcat cgaaagaata aaattcctga tcaatatggc
     4501 agaaacaatc aaagaaacga atgtggagaa acaaacgaca aggatgatcg tgttctggag
     4561 ctttaatggt tttttcttaa aaaaaggaaa cataggctac ctcgaaagaa tttattgtga
     4621 aaattttaac atgaaaacaa aaatttaaca aattgtcaac gataaacaca accttcctat
     4681 aaaaaacacc actgagtcgg taaaagaagc tatacagaat tttcaaacat tggtatgata
     4741 gacgtagaaa tggaaggagg cgtgtatatg atagggggaa tcgcacttga gacgatttat
     4801 tggtatacat tgctgatttg tgcaggattg gcagtcctat tgattatttt tggagatgta
     4861 ttcgattttg atggcccgct tgatccgatg cttattatcc cttggttaac tttcacaagc
     4921 ttatttggtt ttctaggtga gcgtttaaca gatcaatcat ctttattttt atttattgct
     4981 agtggaggca tatcgatagt gttggtattt ttgcttaatt tctatatatt gatgccgatg
     5041 aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc tggaaggaca agtagctgtt
     5101 gtgatcacac cgatccctgt acgaggtatg ggagaaattc agttgaaaag tgttaccggt
     5161 tccattagtc gtccagcttg tttttacagc ccccaagaac agacagctga gcggggaacg
     5221 aaagtattga tcattgagat caaggaaaga atctgttatg tcatgcctta tgaaagcatg
     5281 ataaaagctt aaaggagcgt gtgttaaatg gaaataccag ttgtttttat cgcgatcgca
     5341 atcgcagctt tgattttact gatgttgctg atcgtctttg tagcgaagta ccagacagca
     5401 aaaccggatg aagcgttgat catcagtgga agttatttag gaagcaaaaa tgtgcacatt
     5461 gatgaaagca ataacaaaat caaaattgtg cgtggaggtg gcgcattcgt cttgccagtt
     5521 tttcaacgtt cgaatcggat cagtctatta tcgagtaaat tggacgtctc cacccctgaa
     5581 gtgtatacgg aacaaggcgt accggtgatg tgtgatggta cttcgatcat caaaatcggt
     5641 tcatctgtag aagagattgc tacagcagca gaacagtttt taggcaagac aagagaagaa
     5701 ctggaaaatg aagcaagaga agtattagaa ggacacttgc gctcgattct tggttctatg
     5761 actgtagaag aaatctatca aaatcgcgat aagttcagtc aaagcgtaca agaagtcgct
     5821 tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt tcactatcaa agaggtcaga
     5881 gacaaaaacg gttatcttga ctctttaggg aaaccaagaa tcgcccaagt caaaagagat
     5941 gcagatatag cagaagcgga agctctaaaa gaaacgcgta tcaaaaaagc tcaagcagaa
     6001 aaagaatcac aagcagctga attacagcga caaacagaga tcgcagaatc attaaaagaa
     6061 aaagaactga aattagcaac ctacaaacaa gaacaagatg ttgctaaagc aaaagcagac
     6121 caagcctaca atttagaaag tgcccgagca caacaacaag taatcgaaca ggaaatgcag
     6181 attaaaatcg tagaacgtca aaaacaaatc gaactggaag aaaaagaaat cacacgtcgt
     6241 gaaaagcaat atgattcgga agtgaagaaa aaagcagatg ccgatcggta cgctaaagaa
     6301 caagaagcac aagctcaaaa agtcaaagaa gtgactgaag ccgaagcaga acgattccgt
     6361 gtggaagcac tggcagaagc ggaagccaat aagacacgtt tagctggaca agcagaagcc
     6421 gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa aacaaaagat cgccaatgcc
     6481 ttcaaggaat atggcgaagc agctgtcttg agtatggtta tcgatatgct accgcaattg
     6541 atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa tctctgttgt cgatacagga
     6601 agcagttcag gagaaaccag tggagcgaac cgagtgacaa attatgccac aaatttgctt
     6661 tcttctactc aagaaacact aaaagcgaca acaggacttg acttgaagga aatcgtagag
     6721 aatttgtcga cacgtgggac tactagccaa gtaaatatgt atgaagcgcc aaaagaggca
     6781 gaacaaaact cgatgaagga agaataaagt agtacacttc accgttcaat caaaaatgga
     6841 ggaagacttt ttaaagttct tctctccatt tttttatttt attgcaaaag aaaattagtc
     6901 ctcgctattt ttcttttgtt tttggatctt agcttctatc tcttgtaaat ttgttcttag
     6961 caatcgttct ttcttttgga gttcgaccaa atcttctgat agttcttcct tcttttctgt
     7021 ttcaggagga cgaagaaaga aattgatacc gattgcaatg aatgtcccta taaaagtatc
     7081 tagtacacgt tggatagcat acaaagcgga ttcgccttga gggatgctca acgcaatcag
     7141 caacaacgta gctattgcgg aaatgatacc ggaattattg ttaataccat ctgacacgac
     7201 gatcacaata ataactagaa taggaagcaa gatcaattct accaaaaaat cattgtgaaa
     7261 atagcttttg acaaaaaaat aaatgatagc taatgtacca cctaatgtat tgccgatgat
     7321 cctagacttt ccaaaagaaa cggttgtagt caagtcctga cgcaaagaaa aaacagcaga
     7381 taatgcagca atcatcggga ttcctcgttg agtcaaatga aaaaacaaga tacataaaaa
     7441 aacagctaag gcacttttga tcgtacgcat acctagtcga aaacggccaa attgcataga
     7501 gattctcctt tttggtcttt ttactatcat acctaaactt ttgtctgata gctagttttt
     7561 cattattcaa gtataattaa aataaaaaag ggggaagtaa atgcgtatag gaatgcgaac
     7621 agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca gcgggtagct tatcgctgtt
     7681 gtattggcca gctgcaggga tcattgcatt attaagtgta ggaaatacca agcgctctac
     7741 attgcgtacg ggcatgaatc gaatcgcagc ctttatatta gctacggcaa cagcactgtt
     7801 cagttttttt gctgttgggt atactatttt tggcttcagt ctttttttat tcctgttcat
     7861 cccgcttgcc tctcgcttca aattaaccga agggatagta gtaaattcgg ttttggtcac
     7921 tcattattta gtggaggaaa acatgtcttg gcagctgatc ggcaatgaag cgggattaat
     7981 ggcgattgga ttagtatttg ctttgcttgc aaatgtttac atgccagatc gtacgaagca
     8041 gctgaaggaa aaccaaatac agatcgaaaa agaatttcga aatattttga gacaaatggc
     8101 agaatttctt ttatcagaaa ataaagaaga tgtgcagatc aaatgcgaac atttactgac
     8161 attcattcgc gaaagccaag aagatgccag agaacatcaa gaaaactact ggctcagaca
     8221 gccgctatac tacgaaactt acttctctat gagaagagca caggccaatg tgattaaaga
     8281 catgctggaa aatctagaga gaatccagca accagcttat tatggaaaac atatctatgg
     8341 tttattgatt tatactgcag aaacattttc agagtcaaat gatggcagac aaattttaac
     8401 acgtattgaa gaagtttatg tattgtatcg gcaaatgcct ctgccgacta gtcgccctga
     8461 attcgaggat cgagcagaat tgttccagtt tttgcagtct tttaaaagtt ttattgaaat
     8521 caaggcagaa ttctcccaac aaaaaatcca aaaatagagg ttgtgaattc agctaatatt
     8581 caggtcaaaa agctgatttc acaacctctt ctttaaacgg tgttctcctg tcaactcctc
     8641 ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg
     8701 actcggagct gaacggtagg ctcacaacct ctttcttaaa cggtgttcaa aagctttcct
     8761 ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
     8821 tattacttga tgcagaacag ctttttcaca accttttaaa attacatatt tttccataga
     8881 acttgcattt gatccaagat ttcgatgaca tcttttgtca agaagagaga gcgattgggc
     8941 aagtcacctt caatcatgtg taccatattc tttatttcgt aattcattgc ttcagatgta
     9001 ttcccgcttt cgatgaattc ctttgtacca tctgtaaata agatctccgc acgatcagca
     9061 cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg caacgatacc gacttttggc
     9121 atttttgcct gaaaagtcaa gctgacagca gcaagttcat tttctttgtt acgcaagata
     9181 gtgacggatt gttcgtccac gcctgtttca aaaggaacca ttgtagaggc aactacttca
     9241 ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat acgtaccgat atccaacaga
     9301 gctcctccag ctaattctgg attgaagaac cggtttttag ggtctggttc tttgtaactg
     9361 ccgaaaggtg cttggatcat ttttaaagcc cccagttttc ctgtatccat tattgagcgt
     9421 aactgctgat aaagtggcat gttgaagatt gtcatcgctt ctgctaagat caaattcttt
     9481 tcttctgcta agcgcatagc gtcagccagt tcttttttat tcatcgtgat ggctttttca
     9541 catagaacgt gtttgcctgc ttccaatgct gccaagatat ggtcgatatg ctgtctattt
     9601 ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta acatttcttg atagcttccg
     9661 taagcttttg gaatattgta acggtgagcg aagttttccg ctttttctga agttctggaa
     9721 gcaactgctg ttagttcgct cgtctcttga ttgaagcttt cggcaaactc atgtgcgatg
     9781 gtaccaagac caacgattcc ccattgatat tttttcatct gaaaacgctc cttcatagtt
     9841 ggattgagtt tcttgtctgt ctattattat aacaaaaatc acgaagaata aagaggagag
     9901 gacttgaaac attaaaaaaa atgaaagcag cgatttaaga ggtgcttttt gatcaaaact
     9961 taagtagaat agttaaaagg aggggataaa cgtgcatcaa gtatatattg atcttattat
    10021 gagtgtatta ttagaacagt ttgaaactga aaaaaatttt ttagatgaat acttgctgat
    10081 cagtccagaa caatggcaaa aatggaaaga tggcgagaaa aatctgccat ctgaagcgat
    10141 gcagaaagta aaaaatcttt tcactgatta tgaatggatg ctaacgcaaa agattttgcg
    10201 tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt gcagagtaca agcaaatcaa
    10261 aacacgcatc gcgcaaaaat ggcttagcag caaagcagca aaagtcgagc tgatcccttt
    10321 gaaaaataat cagcaggctg gtagatacct tgacttgaaa gtaagtattc aatatgatga
    10381 atggggctat gatgatattt tgaattttcg tctgccagca gatgttcaaa aacagatcga
    10441 aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat ctggaagaaa cttacgttca
    10501 tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt tcaagtgaat aaatttttag
    10561 gatttggagc agttgtgatt atgatcgcag caatgggcta tgttattttt agcgatgatg
    10621 attcgcatgt tcaagccggc aatcaagcag tcgaacgaac aagcaccaaa acaactgcta
    10681 gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa ggacaaagaa acaaaagatc
    10741 gtacgtccaa acaattgcca gatgttaaaa cagacgattg gaagctagtt ttggttggtc
    10801 cagatcacaa attagaaaaa gaaattgatg agtctactca attggcatca ttaagtaacg
    10861 gatacttgat cgacaaacgc attgctccag aatatgaagc atttgccaag gctgctgaag
    10921 cagctggatt tccattagtg atggtctcag cttaccggtc tgtatcatcc caacaacaag
    10981 ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg tgtatcagaa gaggaggcga
    11041 caaagattac taaagaaacg attacggagc caggatacag cgaacaccac acaggtctgg
    11101 cagtcgatgt cgtcgatcaa aactggtaca acagttatcc ttctactgta ttggatgcaa
    11161 gttacggaga tcagcctgga gctaaatgga tcgcagataa tgctgcgcag tatggattta
    11221 tcgttcgtta tccaaaagga cacgaagata taactaagat cacatatgaa ccttggcact
    11281 tacgttatgt cggtaaagaa aacgctgagt atatcaccaa acaccaattg acgctagaag
    11341 aatatctgaa tgaattaaat gaaaagtagg aattgatcat ggcgaaaaaa aagtggaaaa
    11401 agcaaaagcg caagaatatc cgttggccag cagtggttgc cggtgtagca gtgatactct
    11461 tagcctttgt tttttccttg aagaatctgt cttctccttt tactgataat cagcaagaaa
    11521 atgaagcgca atcccatcag caatttattg aacggcttgt tccgcatgcc caagaactac
    11581 aagatggtta tggagtgctt cctagtatca ttttaggaca agctatatta gaatcaaatt
    11641 ggggaaaaag taccttggct agcaaataca ataatctttt cgggatcaag gcttatgggg
    11701 atcagaagaa agtaagcctg gaaacaaagg aatttgttaa cgaagaatgg ataactatcc
    11761 aaggagactt caaagtgtat gattcatggg aacagtccat ggatgaccat acacaactgt
    11821 tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa agtgattacg gccaccaact
    11881 atcaggaagc cgcacaagca ttgcaagatg caggttatgc gacagacccc ggttatgcgc
    11941 aaaaaatcat ccaggtcatc gagacgtatc aattgaatca gtatgaccgg agatagcaaa
    12001 ggagaagtgg ctgatggcag aacaatttac gccagaaatc atgacggaat tgagaaaaga
    12061 tatcgtaaga gtaccggaaa tcattttaga agcaagcggg atcaagatat ttggaaaatt
    12121 gatccgttcg atcatcttta ctacggatat tgcgatcata cgtaatacaa atgcgaatgc
    12181 agtaattgct gtgtatcctt ttacacctca tccagcgatt actaaaagca ttattgaggc
    12241 agctgacatt ccagttttct ccggtgtagg tggtggtttg acacaaggtg ctcgttcttc
    12301 ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg gtcgttttga acggtcctac
    12361 gcctttagag acagtaaaag aagtttgtcg agtagttgat attccagtta tcagtacggt
    12421 aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct ggtatttcga ttatcaatat
    12481 cagtgcaggt aaagaaactg cagaagcagt acgccatttt cgtaaaaaat atcctgaact
    12541 tcctatcatt gcgacaggtg gtccaacgga agaaagtatt gctgagacca ttcaagcagg
    12601 agcaaatgcc atcacatata cgccaccaag tagcggagag ctttttagtc gtaaaatgga
    12661 aaaatatcgg gaaactgaga aaaagaatca tgaataacag gtgagaatca aaagacgagg
    12721 aagagcagat agtggctctt cctcgtcttt tgtactccag tactatttgt tcagaattca
    12781 aaaaaaagag aaattttttt gtttttttac ctgtaatgta cataagaaga gttaaaacct
    12841 tttgttatta ttagcattct aacaaaaaaa tgtcttgatt tattgaagcg ctttctatag
    12901 aatgaaaaac gaataaaaac taaaaaaggc acccgacaat ttagtagcag ctaaattgtc
    12961 ggggcttgaa aagtcagcgg gaactgacca atcaagttgc catagtcaat gcttgtgcat
    13021 cgacgtgtat attgtaccta atttcataca gaattactag ttatttttct gttttttagg
    13081 gaagagcgca cacgaagaag catgcttatt tagctatgtc aaggatattg ggcagttgtc
    13141 tgatatcctt ttttctttgc gtaattatcc tcccatcatt gaagcaagaa gtgtttttag
    13201 cgatgaaaga agaatcagca ctatctaatt tttcttgaaa gtattgagtt gttcatccta
    13261 ggcagaacga taaggcaagt cttggttttt attccccttg aaaaagatcg tttaataaat
    13321 atgttctgta taaaaataca gtatattgaa aaataacaat ggaagtagag gtgcagagaa
    13381 tgaagaaaag aatgagaatc atcttagctt taagtgttat gttattcttc ttgacggctt
    13441 gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc ttggtggggt tcgcaagtcc
    13501 gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga aaaaaatccc catgtgacga
    13561 tcgattatga attttatgat tatgaaggat attacacaaa attaggtaca ctagcagcat
    13621 cagatgatat ctgggacatt ttccaactga acaatcaatt tcctcaatac atcaatcaga
    13681 ttgaaccatt agatgaatat gtggagcaag ggataattga tatttctaat gcagatgaat
    13741 cctatttagc aacgacgact tacgacgggc aattagttgg actgtctaac ggggtgaatt
    13801 cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt aaatatccct gaaccagctt
    13861 caaattggac atgggatgaa tttgaacaaa tcagctatca aatcactgaa gaattaggaa
    13921 tctatgctat ttcaagattc gaagatttta tagctggctg tattattcaa ataccacaag
    13981 ttgagaaagg attgaatttt tataatcggg aagatctatc taacagcttg ggatttgaaa
    14041 atccagacta tctaacagat tttttccagc tgcgtaaaaa tctggtagac aaaggggccc
    14101 atccagaacc aggtggtgga gctgcaagta cgacggatct gtcttttcag gcggttaggg
    14161 atagagaagc ggcaatggtg tttctatcaa gtaaccagtt gacagaaata ttgtcagggg
    14221 ctcctgaaga tatggagatt cagttgatta atccacctcg cagaaaagca gatggagaat
    14281 ctggtatccc tttacgttct tcgcaaatgc tgagtatggc aaaaagctca gcccataaag
    14341 aagaagctgc taagtttatt gatttctttc aaaacagtga agaagccaat gaaattttaa
    14401 aaggagaacg tggtgtacca atcatgtcga atattcgaga acagctcgct cgtgcagatg
    14461 atgcaacttt gcaagcgaca tttgattatt tagaaatgat ttccgatatg gacaacggcg
    14521 aagtgaatgt ctttgatagt gctttgaatc cggaaattca agaccaatac aatcttttag
    14581 ttgaaaaagt catttatgaa gaagtaacag cagaagaagc agcagagagt ctttatgagt
    14641 ttgcgtcaaa cattctagga tagcagcagg aggaaggcat gaaaaaagaa aaactaaaac
    14701 ggttcttgga tcaagacaat gtggtcggtt acgtatttgc ttctccattc atcctaggat
    14761 ttctattgat aacagtgata ccgatgatct tgtcacttta ttattcgttc accgattatc
    14821 gtttgggaaa tccagtctca tggatcggct tggataatta tataaagatg ctgagtgatg
    14881 cccgtttttt gaactccatc cgagtcactt ttctctatgt atttgcatct gtacctgtga
    14941 aactgatctt tggtctattg attgcttatc ttttaacgag gaatatcaaa ggaacgactt
    15001 tctttcgttc tttgtattat gtgcctagtt tgatcggtgg cagtatttct atcgctctag
    15061 tttggcggga gttgttttct aaaaacgggt tagtgaatat tttcctggaa cgattaggcg
    15121 cacctgcgct ttcctggttt agcgatcaaa aactggtcat gatccctctt gttttgatgt
    15181 ctgcttggca attcggttcg gcaatgatca tctttgctgc aggtatcaaa gagatcccag
    15241 gctcgtatta tgaagcggca gagatcgatg gagcgaacaa atggcagacg ttttacaaga
    15301 tcacgttacc tcttctttcg ccgatcattt tgtataactt ggttatgcag acaatcgcgg
    15361 cgtttatgac ttttacgcag gcatttatca ttacccaagg tggtccgaat gatgccacga
    15421 acttttatgc tctttatgtg tataaccaag ctttttcttt taattctatg gggtatgcta
    15481 gtgctatttc gtggatcatg ctgttgatta tgggagctgt cacctttctg attttcaaat
    15541 catcaaaaaa atgggtcgtt gtgtgaatga gaggaggaag aaatgaataa tacagtaacg
    15601 aaatggtttc aatacggttt tattttgcta ttaggtctag tcatgattta tccggtctta
    15661 tggatgatcg caggttcttt taaaacggat caagagatta tgagtggttc gctgaatttg
    15721 attccagaga cttttcaatg ggaaaattac gcaaaaggat gggcggggtt tgctggaatt
    15781 tcatttttta ctttctttag gaattcgttt gtcatctctg cagtttcgac gatagggaca
    15841 gttctgagct cgacttgtat tgcttacgca ttatcgagaa tcgatttcaa aggaaaaaga
    15901 ttctggttcg tcgtcatgtt agtaacgatg atgattcctg cacaagtgat tttgattccg
    15961 cagtttatta tttacaatcg gctgaatttg gttgggacgt atgtgccgct gattttacca
    16021 catttttttg gacaagcatt tttcatttac cagatcatgc aattcatggt cagcatcccc
    16081 aaagagttag atgaatcagc gattattgac ggatgcagca agtatagtgt ctttaccaaa
    16141 attatttttc ctttgctgaa accttcgatc atcacgacga tcatcatcca gttttattgg
    16201 aaatgggacg acttcatggg accattgatt tatttaaaca agccgagatc ttacacggta
    16261 tcgattgcca tcaaattatt tgcagacgct ggatctagta cgacttacgc gagtatgttt
    16321 gcgatgtcta cgttgtcgct gctaccggtt tttctgattt tcttattctt caataaatat
    16381 cttgtccagg ggatcagtac aagcggtctg aagggctgaa tatgaaaaag ccaatagaag
    16441 agatgtttct ttacaagctc aactactatg tctgtcggat ttttttaacc agtcttatca
    16501 gtaatgctct tttcgcttta gttctaggat gtttgttcac tttaggatta gtgctgtctg
    16561 ctcgtgttgt ctatctgatt tattttttga tgatgtatcc ttcttattat ctatctgtcc
    16621 attacttggc agaaaaaaag gtatgttgga ggaaaggaaa aatcaaacgg atgtgtttca
    16681 tcattacgtt gtttcagctg tttgtcatta tttttgcagt tgattggtat ttattagtgg
    16741 gaaagattgg agcttggtat ctgattcctt ttctattgtg tctgttgctt tttgtatccc
    16801 atagtttttt tagtacgcta attgatatct gcaaagaaga taaagcagat agtcttgttt
    16861 atttgatcaa aaggaatatc tggctgttgt caaaaaacgt caaggggttt ctgtggtatt
    16921 tatgcctttt gtatggactg ttctggtgta tacagcatga ttttttactc actgcattac
    16981 tgtatccagg gctgccattt gtcacagtat tttgcttaaa agaaaaggag aatgacttta
    17041 aatgaagaaa aaatatgtac aagtcggact tggtgggaga gcgcgttttt tttatgaggc
    17101 aattgcctct acgtatcatc aaacttgtga actagtcggt ttttgtgatg tatctcaaac
    17161 aaggatggat tatgcaaatc ggatacttgt gaacaagtat ggacagaaaa aagtaaagac
    17221 atgtatgcca tcacaatttg agcaactact ggatgacgta cggccggctg aagtcattat
    17281 tacgtcagta gatcgaacgc atcatgatta tatcattcgt gcaatgaaaa aaggatatga
    17341 tgttatttgc gaaaaacctc tgactatcga tgaggaaaaa gctcaagaaa taataacggt
    17401 ccaaaaacag acgggaagaa gcctgcgagt cacttttaat tatcgttatg caccgtatca
    17461 cacgaagata aaggaactga tagaaagtga tgtgattggt gatgtgtttt ctatccactt
    17521 cgaatggctg ttaaatacgc aacacggggc agattatttc agaagatggc atcgaaataa
    17581 gcacaatagc ggcgggcttc ttgttcataa atctacccat cattttgatc tagtgaattt
    17641 ttggttgaac agccgtcccc aaaaggtatt tgcattcggt ggattaaact tttatgggat
    17701 gcataatgcg gagaaacgcg gagtagaaat attttacagt cgatgctatg gaagcgaact
    17761 agctaaagct gatccatttg ctatagacct gtcagccaat gaggaattgc gagaaatgta
    17821 tttagaagca gaaaaagaca gtggatatat tcgtgaccgc agtgtatttt ccgatgatat
    17881 ctctattgaa gatacactag gcgtaacggt caaatatgaa actggtgcga ttttgacgta
    17941 ttcgctaaac gcgtatatgc cgtgggaagg attcaatgtt tctttcaatg gcagcaaagg
    18001 acggatcgaa atgaaagtga tcgaaaagtc ttacatcaat gcaggtggag ataaaaagga
    18061 tgaaggagca gccgttcttc agtccatcca tgtctatccg atgttcggtg agccatatga
    18121 ggtaatcgtc gaacaagaag cgggcggaca tggcggagga gatccgcgtt tattagatga
    18181 tctatttggt gaagaaaaag aagaagatcc gtggaatcgg gcagcaactc acgtagatgg
    18241 tattttgtcg attttgacag gaatcgcagc aaatcattct attgcttcag gaaaagcagt
    18301 agtgattgac gagcttgtca gcttaaaaga ataagaatag atttacggaa attacttccc
    18361 gtagtttata aaatcttgcc ttattgccga taggctgtct tttgaacact atttattcgg
    18421 aaaaaggggc tatgacatgc tttttgtcat gccttctttt tatgtgggct gcttttttag
    18481 ataggagaaa ataaaaataa ctatttaaac ccgaacaaaa aggaataatg cattaaaaat
    18541 cattagattt tacaaaaaaa taagtaaacc acgttgtcag tttcagaaag tagtgttaga
    18601 ctgttttcaa tatttgaaac aacatttttt attcaaatat ggcaaactgc cttcacgtgc
    18661 aaaacagttg caagaaaatg aatttacact attattagtt ttacatacct acttatatat
    18721 cgtcataata tggatgacag tttctagcca gtaccgtaaa tgctggacta taagtaaaag
    18781 attggctatt tcacccttct tttcttatga taaagtggag ggtgtttttc tttaacagaa
    18841 acatagtagg tttttgcaca aaagcggtta tttcggtatt aataaatgat aaaggagcaa
    18901 ataaagtgaa agaattagtt gaacgtattc aaaaagacgg acgtatatta ggcgagggcg
    18961 tattaaaagt cgacagcttc gtcactcacc aagtagaccc tgtattgatg gaacaaatgg
    19021 gaaaacggtt tgcagaagta tttaaagaac aaggaatcac gaaagttgtg accattgaag
    19081 cttctgggat cgcaccagct ttatttgctg caagagaatt agatgttcca atgatcttcg
    19141 ctagaaaagc aaaaagtttg acgatggatg aagaattatt gacagcttct gtttattctt
    19201 ttacgaaaca agtgacaagt acgatttcta tttctcgtaa atttttatct tcagaagaca
    19261 aagtattgat tatcgacgat ttcttggcaa acggacaagc ggcaaaagga ttgatcgaat
    19321 tatgccagca agctggagcg caagtagaag gaatcgggat cgtcatcgaa aagtctttcc
    19381 aagatggacg tcagttactt gaagaaatgg gattgagagt cgtttctctt gcgcgtattg
    19441 cttccttgac gaatggacaa gttgagtttc ttgaggagga cgcataatcg tggaaaaaga
    19501 aacacaaaat ggaaaagctg cggttttagg gctgcagcat ctgctagcga tgtatgcagg
    19561 agcagtagcg gtgcctcttt tgatcggtac aggactaggc tttaatcaag aacagatgac
    19621 ttatttgatt tcaattgata tctttatgtg tggattagcg acattattgc aattgacagt
    19681 taaccgattt ttcggaattg gcttgcctgt cgttttagga tgtgcgatcc aagcagtagc
    19741 tccactgatc ttgatcggaa ccaatgaagg agtaggagct atctacggat ctattatcgc
    19801 ttctgggatc tttgttgtgt tagtttctgg tgtcttttct aaaattaaaa aactattccc
    19861 tcctatagta acaggaacgg ttatcaccgt tattggtttg actttgattc ctgtagcaat
    19921 cgagaaaatg ggtggaggta atgcaacagc agctgatttt ggtgacaaaa caaatctgct
    19981 acttgcattt gtgacgattc tcctaattgt aggcgtgcaa atgctggcaa aagggttcat
    20041 tcgttcaatc gcggtattga ttggtttagt cggaggaagc atcttagctg ctttcatggg
    20101 aatgattgat ctgagcgcga ttggagatgc gccgttgttc catgttccac aaccctttta
    20161 ttttggtaaa ccaactttcg acgtatggtc gattttacta atgatcatca tttccatggt
    20221 cagcatggta gaatcaacag gtgtatattt tgcacttggt gacattactg ggaaaaatgt
    20281 tggagaagaa gaattgaaaa agggatatcg tgcagaagga cttgccgtta tcttaggcgg
    20341 tattttcaat acattccctt atactggctt ttctcaaaat gtcggattgg ttcaactttc
    20401 gggaatcaaa acaagaaaac ccatctattt ctctgcattc ttcttgattg ttttaggatt
    20461 attccctaaa atcggtgcgt tagcacaaat catccctgag ccggtacttg gtggaggaat
    20521 gttagtcatg ttcgggatgg tggcagtcca aggaatccgt atgctgttgg aagttgattt
    20581 cagcaatgat aaaaatctat tgattgttgc tgtttctgtt ggatttgggc tgggtttcaa
    20641 tgttacgccg acattattca gtcaaatgcc gcaaacattg caaatgttca ctggaaatgg
    20701 catcgtaatg agtagcatta cggctatcat attgaatttg atctttaatg gattgaaaaa
    20761 agatgaagaa atattggaag aagccttcaa gaagtaatag agcaaaagaa aggttgtgaa
    20821 atcagcgttt tgacctgatt gttagagaaa caaacggaaa aatagttcct cgtattttgc
    20881 cgtttgtttg agctaatacc gagggtcagc tgattgaaca ccgttgaagg aagaggtcgt
    20941 gagcctgccg ttcatctccg agtcacaagg aacaatttca aaaaaaagct tctcatgttt
    21001 gttgaatatt gttccttgtg accgaggagt tggcaggaga acaccgttta tccataaaaa
    21061 ggaatgtgac ctaacttttg tcacattcct tttttttatc tttaataaac acgaacaata
    21121 aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt gattgttaaa
    21181 atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct gtgattatgg
    21241 ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat gaattcggtg
    21301 tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg tttgaatatg
    21361 cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc
    21421 atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat
    21481 cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct ggaggagtac
    21541 ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta cttgcgatac
    21601 agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt
    21661 tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt gcctggttca
    21721 acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa
    21781 atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc acaagttgca
    21841 gattggcata ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct tgccagaaga
    21901 acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta
    21961 cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga ccgcctgtta
    22021 gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac gatcgtcagt
    22081 ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt gaaaaccact
    22141 cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga tctagctcct
    22201 gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc gtttgaaaaa
    22261 gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt tccggtcgtg
    22321 gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc gattgatcag
    22381 gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt gagcctttca
    22441 ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt gaatgaatta
    22501 gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat gagtcagttc
    22561 gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg tttgataagc
    22621 aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg cttgattgag
    22681 aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag
    22741 atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga aatcaaacaa
    22801 acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg ccctgaaatg
    22861 gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga gattttagca
    22921 gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag
    22981 catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc
    23041 gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg ggtccattat
    23101 ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa gcaagcaaat
    23161 gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa
    23221 cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc aactactttt
    23281 ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg atttgaacat
    23341 gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc
    23401 ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca agaaatttct
    23461 actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc gcttattgct
    23521 acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga aacacgtgaa
    23581 gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat
    23641 ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca catggtcact
    23701 gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc agcagaacga
    23761 atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt tggcaatatc
    23821 gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc aacatttggt
    23881 ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac aagagaagaa
    23941 gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt gcagttccgc
    24001 ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga aatagacgat
    24061 gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg agtaggtttg
    24121 gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa
    24181 agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct tatcaataat
    24241 aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata caaaaaatag
    24301 aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg gagatgttgc
    24361 agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat ctggatacga
    24421 taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc gcctttgtgt
    24481 tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt tgctcggcgg
    24541 atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc tttgatcgac
    24601 agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa aaaaagctga
    24661 ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat ccaaattttt
    24721 agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat ggtataagat
    24781 tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg tacatatttt
    24841 tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat
    24901 gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa gaacttttgt
    24961 atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt tggatagaat
    25021 atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag
    25081 aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga ggaataaaaa
    25141 atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta caaattatcc
    25201 ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt ctaggtgttc
    25261 ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat gaccagttgg
    25321 atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaag ccggaagaaa
    25381 tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta
    25441 aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat ggacaaattt
    25501 tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt acgaatgaac
    25561 atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc tatcaagaag
    25621 tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa
    25681 agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag taaaaaaagc
    25741 agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat actttgagat
    25801 acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt gtgacaagct
    25861 gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg aggaagtata
    25921 acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat atgttatggg
    25981 cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag tcctctttag
    26041 aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat ttgagatgcg
    26101 gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct gaagaaggga
    26161 aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga cttttgccag
    26221 gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca ttgaaagtca
    26281 accccaagac ggcctttact tcagagtatg aagccgacga aataatctat cttccagttg
    26341 ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta aaaaaaaata
    26401 atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac attgcaggga
    26461 ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt gcagtagaag
    26521 acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa aacttcggaa
    26581 aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc aagaaaaaaa
    26641 gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa gaagatatct
    26701 taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg agcgaacatt
    26761 gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga cctcatgtgt
    26821 tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta gcagttgtct
    26881 tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga gctgcgacag
    26941 gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt gcgttgctag
    27001 attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta gaagaagtag
    27061 ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc ggcgaaattg
    27121 catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg
    27181 aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc atgtatgtag
    27241 gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa gaatttaatc
    27301 aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg gaaaagctgc
    27361 tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata ggtatacagg
    27421 atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag
    27481 gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca ccttatgaaa
    27541 tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga gaggaagaaa
    27601 aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt aaagtaacag
    27661 acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg ccagtcgatg
    27721 cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct cgaatcgtag
    27781 aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa gtattgcttg
    27841 atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact tatgattcac
    27901 aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg agggttagag
    27961 ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg tatttggatc
    28021 caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt gctagcggtg
    28081 gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa ccagaagttt
    28141 tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc
    28201 ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca atctatccaa
    28261 caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact actcaggaat
    28321 tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat tttgatggca
    28381 gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac tttgatctat
    28441 ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga ttgattgaaa
    28501 gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca gctttcaaac
    28561 atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt gcagaaactc
    28621 aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg
    28681 gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa atttcagcaa
    28741 ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg
    28801 tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg tttggggtca
    28861 tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc gaggacaaga
    28921 aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag atttggggct
    28981 gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag aagctgcaat
    29041 cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc agccttttct
    29101 attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga tcaatgctcg
    29161 ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact ccgatacgga
    29221 gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata aattaaaaag
    29281 tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa cggcaatgat
    29341 tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga aaaacggagc
    29401 atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt ttataagaga
    29461 tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg attgctttac
    29521 gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca gaccagattc
    29581 aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga atttagcgaa
    29641 agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt tgtcagccgc
    29701 aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa aaaatcaata
    29761 cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa
    29821 attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg atgattcaat
    29881 tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag gggcaaaaga
    29941 agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg ggattgatat
    30001 tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat
    30061 cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga tcggtctgga
    30121 aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt atccgacgcc
    30181 gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat cgttctttta
    30241 aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca aaagctggcg
    30301 tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg
    30361 aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat ttatcggtct
    30421 ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg aaattagcag
    30481 tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca
    30541 atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta gcaacgggga
    30601 aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag
    30661 gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg
    30721 attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta ttgacaggag
    30781 aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt cattcaaatg
    30841 ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta tcatcaaaac
    30901 tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa atctatgttt
    30961 cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat atcactggtg
    31021 gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca caaatcaaac
    31081 ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt gaaattcctg
    31141 agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat
    31201 cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg
    31261 ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa cgatgagaat
    31321 cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt atctatcgaa
    31381 aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag catatgtttt
    31441 gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag aatttgattc
    31501 aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga tcgatttgat
    31561 cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt atgataaaag
    31621 aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg gcatacgtga
    31681 tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg accaaggtgt
    31741 agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg acacgttcga
    31801 ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag tgattagtga
    31861 aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt atcagataaa
    31921 actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat catttcaaca
    31981 ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat cgaagaagtg
    32041 acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa aatccatggt
    32101 ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac ggaaaatatc
    32161 caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat tatgaaacca
    32221 gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag tatgctccgt
    32281 agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga ctatgaagaa
    32341 gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca aaaactagcg
    32401 gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata cttaacggat
    32461 tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa acaaacgctt
    32521 cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct tctggaaaaa
    32581 tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa taatatccga
    32641 gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt agtcgccttg
    32701 aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc gtgggaagca
    32761 gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta
    32821 gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat tgccccttct
    32881 tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt actgacagta
    32941 gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt aggtggactt
    33001 cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa
    33061 cgtaagccga ctccagcaga agcaaaagca ttagtgtttg cttggagagc tgtcaaacat
    33121 gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat cggagccggg
    33181 cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa
    33241 ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga atatgcagcc
    33301 aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca agattctatt
    33361 gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca ttttagacat
    33421 tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg tgaacatgca
    33481 ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc gccaggaaat
    33541 ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga tcattcagcg
    33601 ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc tgaagttcct
    33661 ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt cggtccgaca
    33721 aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat ggatacttat
    33781 caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc agcgtatatc
    33841 aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta
    33901 gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat
    33961 cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg agaagaattc
    34021 tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc gcaagatcat
    34081 aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc ttattcacca
    34141 gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg
    34201 gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc gggtttgatt
    34261 gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg
    34321 caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga tttattagag
    34381 aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt tgtggttgca
    34441 gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt tctggaagaa
    34501 gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt tggagcagga
    34561 ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca gaaaaaaatt
    34621 tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga tattggagaa
    34681 aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt ataaaaaata
    34741 caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga gtgtggtaca
    34801 cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat taggtacttc
    34861 tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc
    34921 agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc atgcagatat
    34981 ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa
    35041 acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt
    35101 ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga aaatggatta
    35161 tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg aagtagctga
    35221 agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa
    35281 accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct
    35341 gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca atatgacaga
    35401 tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc atgatatgac
    35461 gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta tcgggaacga
    35521 aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc aaacgacacc
    35581 aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag tgatcgccgg
    35641 tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta atatgtctcg
    35701 ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa gattgtcata
    35761 agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg ttttcagctt
    35821 cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg gatttcgttt
    35881 attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga gaaatccctt
    35941 gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa
    36001 tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg attctttgtt
    36061 ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta ctgaaatgag
    36121 agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga tcggtaaaat
    36181 tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc cttgaaggct
    36241 gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg taaagcgagt
    36301 cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt tcttgcggga
    36361 ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat aaacgatata
    36421 atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt
    36481 cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg
    36541 gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta agggaactgg
    36601 cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg attctttctg
    36661 cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat tcatggacca
    36721 acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa tcaactatta
    36781 aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc tccctaaata
    36841 aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg acttggtttg
    36901 ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat tcagcaagtt
    36961 tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa tgattttttt
    37021 tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag
    37081 ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta agcagataat
    37141 catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat ccactaataa
    37201 atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca
    37261 tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata attgaagaca
    37321 gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa gaaccaagga
    37381 cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt
    37441 tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag tgtgacagtt
    37501 gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc gtaataaaga
    37561 aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga atcgatccct
    37621 ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat cccgttgtct
    37681 ataactttga attggatcat ctcatcaatt ttgcatgtgg taatacgaat aaatcctttg
    37741 tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg caagatcatt
    37801 tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc aagcatatgg
    37861 ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc tttttcaaga
    37921 ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc
    37981 atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc atccaaactg
    38041 ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact ttgtgctaaa
    38101 tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc cattttattc
    38161 atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg aaagccaagg
    38221 ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc ttggaccttt
    38281 ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag aactccaagt
    38341 tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt gcttgctaag
    38401 tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt gacagaatct
    38461 acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat attattgatc
    38521 agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc taacgtacta
    38581 atttctttaa gtgtatttgt attatttttg ctctctcgaa tatcattcct aagttcttct
    38641 aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa gtcccacata
    38701 ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg gatatttgcg
    38761 atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag tagcaaaggg
    38821 ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa
    38881 ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag ctatattctg
    38941 ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc ctgtttgaat
    39001 atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag gtttacactc
    39061 gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt gtccatcaat
    39121 agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg cagctaaagc
    39181 accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata aatcatttgt
    39241 ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac cgacaattcg
    39301 gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta gggttggttt
    39361 acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg agttctctga
    39421 accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt gattgctgac
    39481 aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag
    39541 ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc ctgctcgctt
    39601 tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc
    39661 cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat tcatgtagcc
    39721 atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg attcagtatg
    39781 ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg
    39841 tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa caagggctaa
    39901 aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt actatcttat
    39961 cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa
    40021 aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc
    40081 aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg gggataaaaa
    40141 atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc aagcattcta
    40201 ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt aggaatcgct
    40261 atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt ctcggaatta
    40321 gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga aaatcaagta
    40381 gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc ttctatcgat
    40441 ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc
    40501 tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt tgataatttt
    40561 ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa agaaggaaaa
    40621 ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc actaatcaat
    40681 tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt ggtcgtgcca
    40741 tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc tacggcgcaa
    40801 gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga tgctgttttg
    40861 tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt ggggtatggg
    40921 agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc tggcgttaaa
    40981 tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat cttagcaaaa
    41041 aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt caatgatact
    41101 gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac agtatctgtc
    41161 gataaagata attatcaagc agagctgatc gacaccgatt attataaaga atccgatctg
    41221 aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag aggataagaa
    41281 ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct ggaatccgag
    41341 cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg tgcggagaaa
    41401 acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca tatggctcct
    41461 atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg aaagatagtg
    41521 aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat ctttcaataa
    41581 aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat tgggaactga
    41641 cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac ccgaatcttc
    41701 tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa gcattatcaa
    41761 aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg
    41821 cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc atcattattt
    41881 cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta cgggatggac
    41941 agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt cgcggaatgg
    42001 tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga gaagtttatt
    42061 ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc ataaacgacc
    42121 acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg atgggagcgg
    42181 gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag atatccggac
    42241 gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc attgcaaacg
    42301 gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg atggatattc
    42361 gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta ttagataaag
    42421 aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca
    42481 tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt
    42541 ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg atagatgttg
    42601 gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc aaagctgtct
    42661 gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc tatacgatga
    42721 acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa gtcttgatga
    42781 aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct tacggataaa
    42841 aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg ctacagcatg
    42901 gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg tattttctgg
    42961 cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt actagctgca
    43021 gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc agtgatggcg
    43081 tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca
    43141 gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt ttgggtagct
    43201 tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt tcgtggactg
    43261 acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg
    43321 tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt gacattgatt
    43381 ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg
    43441 aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc ggtatttaca
    43501 gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta tccgattatt
    43561 ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa aacagtagca
    43621 ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa
    43681 acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc tttagctgga
    43741 ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc gatggaactg
    43801 gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat tggaacaatc
    43861 atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat gtcgatttta
    43921 ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt agccgttgtg
    43981 ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa
    44041 agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca ttcattgttt
    44101 cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat tctctttttg
    44161 ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa agccttgatt
    44221 aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg taatgaaagt
    44281 atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata gccgttctga
    44341 atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag tgcctgccaa
    44401 catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg gttatttcta
    44461 tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg
    44521 tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct ttatagagaa
    44581 gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc atggacatgc
    44641 taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat
    44701 tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc
    44761 aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga caggctttgg
    44821 gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc gcaagccgat
    44881 catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc gttttggtgc
    44941 gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa
    45001 agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat tccaaaaagg
    45061 cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga
    45121 tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg gaggaagaga
    45181 tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt ttaatggaga
    45241 cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc tgcattaagt
    45301 aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg aattattatc
    45361 gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa gatttttgtc
    45421 acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca
    45481 aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa
    45541 ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa cggaaggatc
    45601 aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg tgtgaatcgt
    45661 tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac gagcaaacgt
    45721 ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc aagaatcgat
    45781 tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca aaaaataaga
    45841 gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga gatgccagat
    45901 gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt agcggaagaa
    45961 aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg gtatttggaa
    46021 agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg cggtcagcaa
    46081 gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc tgtagagtca
    46141 ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt atttgggata
    46201 ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca taaaaataaa
    46261 ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt gatgagtcaa
    46321 aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat tattgaagac
    46381 gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat acgaacgatt
    46441 gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa
    46501 atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc gcaagaaatg
    46561 atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt taatgaagca
    46621 attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg actgataagc
    46681 gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg ttactatgcc
    46741 tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt agtagatatg
    46801 ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat cagaatcaat
    46861 tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa aattctagaa
    46921 aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac agcttggatt
    46981 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
    47041 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
    47101 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
    47161 aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac taatgctgtt
    47221 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
    47281 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
    47341 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
    47401 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
    47461 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
    47521 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
    47581 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
    47641 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
    47701 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
    47761 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
    47821 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
    47881 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
    47941 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
    48001 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
    48061 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
    48121 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
    48181 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
    48241 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
    48301 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
    48361 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
    48421 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
    48481 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
    48541 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
    48601 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
    48661 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
    48721 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
    48781 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
    48841 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
    48901 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
    48961 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
    49021 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
    49081 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
    49141 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
    49201 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
    49261 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
    49321 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
    49381 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
    49441 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
    49501 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
    49561 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
    49621 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
    49681 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
    49741 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
    49801 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
    49861 ggagtcttcc aatttaagtt
//
LOCUS       DAJSVD010000009        47605 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSVD010000009 DAJSVD010000000
VERSION     DAJSVD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.009"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(188..922)
                     /locus_tag="ON498_000413"
     CDS             complement(188..922)
                     /locus_tag="ON498_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3938713.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1249..1659
                     /locus_tag="ON498_000414"
     CDS             1249..1659
                     /locus_tag="ON498_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3938714.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1652..2353
                     /locus_tag="ON498_000415"
     CDS             1652..2353
                     /locus_tag="ON498_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR3938715.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2353..3939
                     /locus_tag="ON498_000416"
     CDS             2353..3939
                     /locus_tag="ON498_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3938716.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3958..4179
                     /locus_tag="ON498_000417"
     CDS             3958..4179
                     /locus_tag="ON498_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR3938717.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4176..4937
                     /locus_tag="ON498_000418"
     CDS             4176..4937
                     /locus_tag="ON498_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR3938718.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5317..5826
                     /locus_tag="ON498_000419"
     CDS             5317..5826
                     /locus_tag="ON498_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3938719.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5901..6440)
                     /locus_tag="ON498_000420"
     CDS             complement(5901..6440)
                     /locus_tag="ON498_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR3938720.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6580..7191)
                     /gene="rpsD"
                     /locus_tag="ON498_000421"
     CDS             complement(6580..7191)
                     /gene="rpsD"
                     /locus_tag="ON498_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3938721.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7453..8304
                     /locus_tag="ON498_000422"
     CDS             7453..8304
                     /locus_tag="ON498_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3938722.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8332..8967
                     /locus_tag="ON498_000423"
     CDS             8332..8967
                     /locus_tag="ON498_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR3938723.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8987..9760
                     /locus_tag="ON498_000424"
     CDS             8987..9760
                     /locus_tag="ON498_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3938724.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10168..10965
                     /locus_tag="ON498_000425"
     CDS             10168..10965
                     /locus_tag="ON498_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR3938725.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10943..11356
                     /locus_tag="ON498_000426"
     CDS             10943..11356
                     /locus_tag="ON498_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3938726.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11340..13985
                     /gene="mutS"
                     /locus_tag="ON498_000427"
     CDS             11340..13985
                     /gene="mutS"
                     /locus_tag="ON498_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3938727.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            14003..16111
                     /gene="mutL"
                     /locus_tag="ON498_000428"
     CDS             14003..16111
                     /gene="mutL"
                     /locus_tag="ON498_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3938728.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPESDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16133..16693
                     /gene="maf"
                     /locus_tag="ON498_000429"
     CDS             16133..16693
                     /gene="maf"
                     /locus_tag="ON498_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR3938729.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16778..18766)
                     /gene="treP"
                     /locus_tag="ON498_000430"
     CDS             complement(16778..18766)
                     /gene="treP"
                     /locus_tag="ON498_000430"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR3938730.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18989..19702
                     /gene="treR"
                     /locus_tag="ON498_000431"
     CDS             18989..19702
                     /gene="treR"
                     /locus_tag="ON498_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR3938731.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19779..20225
                     /gene="msrB"
                     /locus_tag="ON498_000432"
     CDS             19779..20225
                     /gene="msrB"
                     /locus_tag="ON498_000432"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3938732.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20395..20997
                     /gene="ruvA"
                     /locus_tag="ON498_000433"
     CDS             20395..20997
                     /gene="ruvA"
                     /locus_tag="ON498_000433"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3938733.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            21010..22011
                     /gene="ruvB"
                     /locus_tag="ON498_000434"
     CDS             21010..22011
                     /gene="ruvB"
                     /locus_tag="ON498_000434"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3938734.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            22040..22618
                     /locus_tag="ON498_000435"
     CDS             22040..22618
                     /locus_tag="ON498_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3938735.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22670..23152
                     /locus_tag="ON498_000436"
     CDS             22670..23152
                     /locus_tag="ON498_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR3938736.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23269..23643
                     /locus_tag="ON498_000437"
     CDS             23269..23643
                     /locus_tag="ON498_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938737.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24321..25673)
                     /gene="brnQ"
                     /locus_tag="ON498_000438"
     CDS             complement(24321..25673)
                     /gene="brnQ"
                     /locus_tag="ON498_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3938738.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25820..26410
                     /locus_tag="ON498_000439"
     CDS             25820..26410
                     /locus_tag="ON498_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3938739.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26538..27887)
                     /locus_tag="ON498_000440"
     CDS             complement(26538..27887)
                     /locus_tag="ON498_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3938740.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(28055..28795)
                     /locus_tag="ON498_000441"
     CDS             complement(28055..28795)
                     /locus_tag="ON498_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3938741.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28808..30400)
                     /locus_tag="ON498_000442"
     CDS             complement(28808..30400)
                     /locus_tag="ON498_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3938742.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30968..31264)
                     /locus_tag="ON498_000443"
     CDS             complement(30968..31264)
                     /locus_tag="ON498_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938743.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31440..32165
                     /locus_tag="ON498_000444"
     CDS             31440..32165
                     /locus_tag="ON498_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3938744.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32158..33000
                     /locus_tag="ON498_000445"
     CDS             32158..33000
                     /locus_tag="ON498_000445"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR3938745.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            33003..33524
                     /locus_tag="ON498_000446"
     CDS             33003..33524
                     /locus_tag="ON498_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR3938746.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33777..34340)
                     /locus_tag="ON498_000447"
     CDS             complement(33777..34340)
                     /locus_tag="ON498_000447"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3938747.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34591..34860
                     /gene="rpsO"
                     /locus_tag="ON498_000448"
     CDS             34591..34860
                     /gene="rpsO"
                     /locus_tag="ON498_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3938748.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            35048..37174
                     /gene="pnp"
                     /locus_tag="ON498_000449"
     CDS             35048..37174
                     /gene="pnp"
                     /locus_tag="ON498_000449"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3938749.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37684..37950
                     /locus_tag="ON498_000450"
     CDS             37684..37950
                     /locus_tag="ON498_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3938750.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            38127..40085
                     /locus_tag="ON498_000451"
     CDS             38127..40085
                     /locus_tag="ON498_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR3938751.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40439..41740
                     /locus_tag="ON498_000452"
     CDS             40439..41740
                     /locus_tag="ON498_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3938752.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41870..43240)
                     /locus_tag="ON498_000453"
     CDS             complement(41870..43240)
                     /locus_tag="ON498_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR3938753.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43422..43745)
                     /locus_tag="ON498_000454"
     CDS             complement(43422..43745)
                     /locus_tag="ON498_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3938754.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(44070..44678)
                     /locus_tag="ON498_000455"
     CDS             complement(44070..44678)
                     /locus_tag="ON498_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR3938755.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            45008..45724
                     /locus_tag="ON498_000456"
     CDS             45008..45724
                     /locus_tag="ON498_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR3938756.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45737..46432
                     /locus_tag="ON498_000457"
     CDS             45737..46432
                     /locus_tag="ON498_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR3938757.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46516..47145
                     /gene="udk"
                     /locus_tag="ON498_000458"
     CDS             46516..47145
                     /gene="udk"
                     /locus_tag="ON498_000458"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3938758.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15427 a         8809 c         9905 g        13464 t
ORIGIN      
        1 acaagctcgt ttggtgtaag atgggtataa gtcatttatg ttcactctcc ttgtatgctt
       61 tagcgggtat tacaatttga gtgtaacata aatggctttt ttatttgtct cgcttaatta
      121 tacaaacggc gaaataaaaa aacttggtac gcaatcgctg taccaagttt ttttatttag
      181 cttttgatta agccataaat aataatttat ataagaatac tgctacgaca ccagcacaga
      241 taggtgctac aactggtacc catgcatatc cccattgaga agaaccttta tgtttcaatg
      301 gaagaagtgc gtgtacaaga cgtggagcaa agtcgcgggc tgggtttagt ccaggacctg
      361 taggaccacc aagtgaagca actaatgtcc atacaaggaa accaagtgct aagtgagctg
      421 taccaagatt atcttggaag aatggtgctt ttgtaatacc tagggcacca aagaataaaa
      481 cgaatgaacc aaagaattca ttgacgaaac cattgaattt agaaccaacg ctgttgattg
      541 ttgagaatgt acctaacaca tggtttacgt cttctgtttg gtcgtagtaa ggtttgtaac
      601 aagctactac gatcaattgt ccaatcgctg caccaatcaa ttgtgcaatg atataaggaa
      661 caacttcagc ccaagggaat agaccattag atgctaaagc aattgtaaat gctgggttga
      721 tgtggttacc gctgattcca ccaaaaatca ttgctggcat cataaccccg aatccatacc
      781 cgacagcaat cagcatccag tcactgccat atccttttgt accttttaaa tctacgtttg
      841 ctacagcacc gttacctaga acgattagta atgctgtacc aatcatttcc gccgcaagac
      901 gggtcattag tgagtaatcc ataaatacct ccaaaagtta taaaaatgca aaaaaaaaag
      961 aaacatcaac acgaaaagca tcggtgcagt gtttcgtgtc ggaaggattt ctgcagtctt
     1021 aacaaatgtg aataaaaaaa tgaattccat tacttataag aaaccagacc aacagtaaaa
     1081 ctccaactct ttttaaaagt tctccacaaa tcaccaagct taactataaa tataccaaaa
     1141 aaaaaacgtt ttttttctca aaaattgtat cttagaaaca gcatgttttg aaactttcag
     1201 aaaaggtttg ttagtaaaat gaaagctata cgtaaggagt gttgtttgat gaaatttttt
     1261 caagagtttt tcattattat tttcttttct gtattaggag aaggcatacg gatttttgct
     1321 catttaccta ttcctggaag tatcttaggt attctttttt tatttttagc atttgaaatg
     1381 catcttgttg atcctgaaaa aatcggagca actggtgatt ttttattgaa taatctagcc
     1441 attttatttg ttcctgctgg tgtaggttta ttggaatatt ttgatgatat tgcttcgata
     1501 tggcctgttt tactgggagc agtcgtcgtg tgttccatcg tcacgatggt tgcagcggga
     1561 aaaacggcag aaggtgtaga agctttgctg ggctatgtcc gtaagaaaaa agcagaaagg
     1621 gtatctgtaa acgaaatgga ggagaatcaa attgactgag ttttttgtga atccattatt
     1681 tggcttaagt ttgactttag gagtgtacgt attatctgac aaatttttgc ggaaatataa
     1741 actgcctgtt ttgaatcctc tcgtattatc gattgttgtt attatcttat ttttgcattt
     1801 ttctgggatc agctataaag actataatat cgggggcaat tttttgacta tgctgattac
     1861 tcctgcgaca gtggcacttg ccatccctct ttataaaaac ttccgtttac tgaaagagaa
     1921 tttttatcct gtaatggctg cgattttagc aggaatagtc gcaaattgct tggttagtgt
     1981 agggttcggt tacttcttct ctcttcataa agagatggtg atctcattac tacctaaatc
     2041 tgtaacgacg gctatttctg tcgatttatc tcatacgatg ggcggggtga atgccgtgac
     2101 acttgctatt gttgtctcta caggtatttt tggttcgctg atcgccactc atattttccg
     2161 cctattcaag atcgaaagtc cagttgctcg cggtgtggcg ttaggaagta cttctcatgc
     2221 tattggtaca gcaaaagcaa tcgagattgg tgaaatagaa ggaatcattt ccggacttgc
     2281 gatatgcgtc aacggaatat tgaccgtttt attgctaccg ttattctttc agccttttgc
     2341 tggcttgttt tgatgcgaaa accactattt tatgctacaa taaccaaaac gttcgagcat
     2401 aaaaaaggag ggaaagacgt gtatttgtct tccgagtatc ttaaacaatt tgataaagat
     2461 ctatattata agattctcct cctagaaagt tttgaggatc aagcttggca taccgctgca
     2521 caattagcgc aagttgttca gctggatgcc cgcagcgtgt cgaaatacct gaacgagctg
     2581 tctaaaaact atcagcagtt tagcggtaag acacaccctt tgtttacaaa aaatcaccgt
     2641 tctggttata acttttatga tacgctcgat tcaattgaac atgagcgttt tttgatctat
     2701 ctcgtgcaaa gtacgctgaa attccagttg ctgcacgaca ttttttttga agaattccat
     2761 acaatgtacc aatttgcgca aaagcattac attagtgaat ctactgctca tcgaaaaatc
     2821 aatgaatgga aacagcaact gcaaacttac ggaattcggc tgcaacgagg tacatatatc
     2881 gctcaaggcg aagaagaaat cattcgactt tacttgcata tgacattctg gcagttgttt
     2941 cgagggaaaa tctggccatt tgaaacgatt tctcaaatgg acgtaaagaa tatggcagaa
     3001 catatcatgg ctttttttaa tgttcgtctg aacgagatca aaaaaagacg attggaatat
     3061 atgctaggtg cctttttttt aagaaaatca cagaagcatt atgttgtatt gaatgagaaa
     3121 aaaagacggc tgatttctga caatctgttg ttccagcgat tttgccaggt catggagccg
     3181 gtatttccta attattttca agtagaagac gagttaggcg cattatttct tgtactgatg
     3241 actcgtgaag aatattatag tgatcctaag atacggaaga aaatttttga ttttcatcaa
     3301 caagcaaaaa caccgccctt tacagccctt tcagaagcaa aagccgctct ttctctttat
     3361 caggaagagc aggggctgcc agcagaaaat ctaaccttcg aggcagaaaa ctatttattc
     3421 agcagtcatt ttttcgccta tctttttcct aatgcgaaag aaacgattga tggcaatagt
     3481 agtgatttta ttaatcattt agttattgaa aacaaggaat tgaaacaatg gctcgtacat
     3541 ttttttgaaa gtagacacaa acatcccaat catctggcat ttaaaaatca tgcgtttttg
     3601 atggggcggt atctgactgt gtttaaaaca ttaggagcct ttactccaca gcttccaaag
     3661 atcacgatcc ttttaatgac agatttccca ttatttgaag agcaactgtt agaggaggga
     3721 ttacggacat ttttccgtaa tgagtatcag ctgatttttc tgccaacaga ttatcggggg
     3781 agagaagttg atcttctgat ttcgacaagt aaagtccacc gcaaaccatg ggcggatctt
     3841 gactatttta ttgtgactga agaactaaaa ttaattgatt acatccaact atctcaaaaa
     3901 tttgaaatga ttcaaaaaca aaaactatta aaacaataac aaagaaggag aaaaaagatg
     3961 acatttgaag aaattttacc agaattgaaa aaaggtgcaa agattattcg taaaggatgg
     4021 agtggatttg aactttatgt tgttctcgta tccgatgatg aatttgatgg ctgtccggtg
     4081 acgccgtact tattgatcaa aacaagtgac gaaggttttt ccagcttcgc tcctacagtt
     4141 tgtgatattt tagcagatga ttgggcgatc gtagaatgaa aaaccagtat ccagatctag
     4201 ctaaacaggt tgtatttatc acaggagctg cttctggaat tggtgctgcg caagcccgag
     4261 cgtttcttga acaagatgct tttgtatttg gattagatca aaagttcggt aaaatggaag
     4321 aaatttcttt gcgatttcct gaaacttttg cctacgcact aggtgatgtt cgacaaatga
     4381 aagaactcca acaagcggta tcgaaatgcc aaaatcattt tggtgaagtt acgattttac
     4441 tgaatacagc ggggatattg gatgcctaca aacccttgat ggaaactgac gaatcgctgt
     4501 gggatctgat ttatgagacg aatgtgaaaa gcatgtatca gctgacaaaa atcatccttc
     4561 ctgatatgat ccgccaaaaa agcggaacga tcatcaatat ggcttcgatt gctggcttga
     4621 ttgctggtgg aggtgggatc gcttatacaa gcgcgaaaca tgcgattgtc ggatttacca
     4681 aacaactcgc tctggattac gcaggacagg gtatccgagt aaaaggaatt gctccaggag
     4741 ccatccaaac accaatgaat gcggctgatt ttgaagggaa aggtgagatg gcagagtggg
     4801 tggcaaatga aacaccagtc aagcgatggg cacagccgga agaagtagct gagctgacgt
     4861 tatttcttgc ctctcctcaa gcaagctaca tacaaggaac gatcatccca atcgacggcg
     4921 gatggttgtt aaaataacct tgtgtttttt tcaagcttct gtatgataaa actaaagaga
     4981 gaaacaacgg ttgatgataa aaaaagtcga tgactgttcc cgacattcga tgccgaacag
     5041 ccagttcaaa aaattctttt taataatcgt taatattcga aactaagcgg cttttcacga
     5101 cctcttgata taaacgatat tcaaaagctt gcaactgcgg taacaagtcc agccaaatga
     5161 aaaatataag gagctatttt tatttgacca ttcttattac ttgttgcagg gcagcttttt
     5221 cactaccttc tgcgacactt cagttggaat tttcttccca tcctttggtc gcggtcatta
     5281 ccaagataaa attatcttgc aaaggagaaa tagaacatgg aaaaaaataa tacccgttca
     5341 gccaaacgct ggacaacgac agatgtgact cggatggcgc tagtcactgg tttatatgtg
     5401 gcagtgacgc tggttttgtc tgtctttagc tttggagtga ttcagattcg tctctcggag
     5461 atgttcaact atctgtcctt gtataacaaa agatacatcg tcgcggtaac agcgggagta
     5521 gctttggcta atattgcttc tcctcttgga ctgatagatg ttgcagtagg aagcgtcagc
     5581 acgctgcttg tattgcttat caactataaa atcactagtc gcatcaaaaa catgaaaatc
     5641 aaaatggccg tgacagcatg cgtatttgct ttttccatgt ttacagtagc tggtcagcta
     5701 acgattttat atcaactacc ttttttcttg aattggtggg tgattgcttt aggagaattg
     5761 gcatcaatga ttgtcggtgg gataattatt tattggatcg gtaaaaaagt ggatttgtca
     5821 aaataaagat ctgtttaatt taaaataggg cagcatggaa gagactgaat gttgagtctt
     5881 cttccatgct gcttttttga ttaatttgta tgaagaaatc gatttagccg ttttttcatc
     5941 aagataccga tataagctgc tccagttgcc ttgattgctt ccggcagtaa aaacggcagt
     6001 agtccactgg caaatgcagt tgtaaaagta agatcaccac ttattttcag ccagatcgtt
     6061 ccgcagatca atgcagcaag agagcccatt agattggcaa agattgcata taagtaatga
     6121 gcatccgttc tttcgatcat ccaaccagtt atccacgttt gtaagagaaa tccaatcaaa
     6181 tatccgccag ttggcccaaa taaaacagct atacctccag tcatccctga aaatacagga
     6241 agaccaatca agccaagtaa aagataaact ccaacagata tcaatcctag ttttcttccg
     6301 agaaaagttg ctgttaatcc aatagcaaat gtttgccctg tcaaaggaac caaaccaaaa
     6361 ggaatggtca tctgagaaag gacagaaata atcgcggcaa attccgcagc aagtatcaat
     6421 tctttcaatg ttcggttcat aacgccctct tttcgttaac ttaatatttt tatttggtta
     6481 acgaaattgt aaaacaaagt gattactttg taaatagaaa atataaaaaa agaccagcta
     6541 tccaatagat agcggtcttt ttcaagaaag cttttgaaat tataagcttt ggttgtagta
     6601 ttcaacgaca agagcttcgt cgatttctgg atatagttcg tcacgttctg gtaaacgagt
     6661 caatgaacct tccaattttt cagcgtcaaa gcttacgaat gctgggcgtc caacagttgc
     6721 ttctacagct tcttttactg ttacgatgtt ttgtgatttt tcacgaactg agatcacttg
     6781 acccacttct acgtggtatg aagggatatc aacgcgtttg ccatcaacta aaatgtgacc
     6841 atggttaact aattgacgtg cttgacgacg agtcgttgca agtcctaaac ggtaaacaac
     6901 attgtctaaa cgttgttcta gtaagatcat gaagttaaca ccatgtttac cttctttgat
     6961 tttgctagct tttacgaaca agttacggaa ttgacgttcg ttcattccgt acatgtgacg
     7021 aagtttttgt ttttcagtta attgcatgcc gtattctgat tgtttgccac ggctattagg
     7081 tccatgttga cctggtgcgt aagggcgacg tgctaattct tttcctgtgc ctgacaatga
     7141 gatgcctaag cgacgagaaa ttttccatga tggtcctgta taacgagaca ttaaaatttc
     7201 ctccaatgaa tataagtttt tggagtaaaa taatccgttg aaaaattcat aatcatatag
     7261 tttgttcttc aatcttcacc tttgcagccg cggttacaca atcgaacccg agacggggca
     7321 acaaactagg gatgagttat tatttttctg ctgcattatt ttacacaaat tacagtatat
     7381 gatagatcat ccacgaatgt caagataatt agtacataaa aacgctttac aaacctaggc
     7441 tgtctttata aaatgagagg gtatgtgcaa atggaaaaaa gcgaaggaag tttaaggaga
     7501 aaatcaatga atccggtatc accattattt gagaaaatcg atgcttcaat tgaaaagaag
     7561 gcgaatcttt ttgaaaacag cttttcacgt tacgctgttc gagcggttct cgcctgtttg
     7621 tttttgactt taggaacggc tgtggcattt gcgatcgcga tgaaggggga agatatcgcg
     7681 catggtctag gaaagatttt atacgcattt atgttcagct ggggtctggt catgattcta
     7741 tacatgaacg ccgaattagg aacatcgaat atgctttata tgaccgtggg tgtttttcgg
     7801 cagagacttg ctttaagtaa ggcagccaag attctatttg cctgtatttt tttcaatatg
     7861 attggcggta tattatgtgg atttctaatt tctatgacta cgccatttca agatttaacg
     7921 ttggacaact ttatgttaga atcaatagca gtaaaactag caaaaccttc agcaacgatt
     7981 ttggtggaag ggatgtttgc gaatatcgtt gtcaataccg ctgtactgat cagcatgcgg
     8041 atgaaagagg atgtcggaaa agtgatcaca gtcgtattca tcattttcat ttttgctttt
     8101 ttaggatatg aacacgtgat agcgaatttt cctgccttta cgcttactta ctttgcttct
     8161 cacggacaga tggatggaat gacagtcagc aatgtgttac ataatttgtt ttttgctttg
     8221 gcaggaaatt atattggtgg cggtttagta atgggactgg gatatgcttg gctgaatcag
     8281 tcaaaatcat cctatgtgga ctaaaaagtc gttcattaaa ggggaagtgc catggaagtt
     8341 ttagtttttc caaaaaatga ggaaaaagcg ttacaaatgg aaaagtacat gaaaaatctt
     8401 ttttcctttg ctggagtacc taagccggaa cgcgctcttt tacaacaacc gttactggat
     8461 gagagcaaaa atttatctat ttctgaagtt ttagcttttg ttcattatta ttatgaacaa
     8521 atcgaaagag agtatcagta tgtagcgatc gatttagtcc agaaaaacat caaacggctt
     8581 acatttgaag acttagtcgt attgttgcca ctagttcaga aaaaagaatg gtgggacagc
     8641 atcgactctt ggagaaaagt atacagcaca tggtgtaaag cctacccaga gcagcgtgaa
     8701 gaagtctttc aattgtttga aaaaaaggaa gatttttggc tacgtcgaat agcaatcacg
     8761 ttgcaattag gttataaaca gcaaacagac acaggactat tgatccgagc aatagacgaa
     8821 gatcgtcaaa caaaggaatt ctttatccaa aaagcaattg gttgggcatt acgcgattat
     8881 agtaagaccg atgccgattg ggtaaaaaga cagctgacaa cagatttgag taagttagct
     8941 gttcgagagg caagtaagta tttataagaa agcgagacga caagagatga gacgcaaatt
     9001 atttgctttt gatattgatg gaacgttgtt agattcaaac aaacaagctt tagatagcac
     9061 aagagaagca ttggaaaaat tacgcaaagc cggtcatctg gtcacgattg ctacagggcg
     9121 gagccgattt catgcgcaag agatcattcg cgatcttgat tttacgaact acattttatg
     9181 taatggagcg gccggttttt tagaccatga acaagtatat aagaatttac tagatcgcga
     9241 ccagttggat gcgttcgttc aagaagcgca tcatgagcag atcgatacag cttttgtcag
     9301 cttagacaat atcaagcgtt cttcttccaa tgatgtaacg gtgatggaag aagcgatgca
     9361 ttctttcggt gctgtcttgc ctgaattaga tcagtttttt gcagaggaac aagaaatata
     9421 ccaagcgtta gcatttttcg atcaaacata tgatgggaag ttctcttcat atgataaaat
     9481 tcgatttgtc cgctggcatg agaacagtgt agatgtcgta cctaaaaatg gttctaaagc
     9541 agcgacaatt ctgcacttag cagaacaagt aggcatccct catgaagaca tcatcagctt
     9601 tggcgatgga caaaacgacc gtgaaatgtt gcgcatgtca ggaattggga tagctatggg
     9661 gaatgctgtg ccagaagtac aagcagaagc taagatggtc actgatacaa acgatcagga
     9721 tggaatttgg aaggcattga aagagttaag cgtgatttaa aagaggttgt gaaaaagctg
     9781 ttctgcatca agtaataagc ccagccaaac aaaaatagct cctcctattt ttcgtttttc
     9841 tgggcttatt accgcagatg caagctttcg aacaccgttt aaggaagagg tcgtgacaga
     9901 agtggtcagt ttcaagaaat aagaagggat ttacgaaaat tgcttctcaa atttttgtaa
     9961 aattacggct tatttctgaa gaagtagatg aaacaacgtt tattcgggaa atgggactgt
    10021 gacaagatgt ttgtcatagt cccatttttt tattttattt ttcaaaacta agaaatacca
    10081 ggaaagagtt ccttttgttt gaccttttct ttcttattga aaacaactat aatatagagt
    10141 gatgttaaaa gagaagagga atttagtgtg cgggtattgt ttattggtga tgtagtaggt
    10201 tcaatgggac gagagatgat tactgaatat cttcctagat tgaagaagaa gtaccgtccg
    10261 caagtaacga ttgtaaatgg tgagaatgcc gcttctggac gtggaatcac agaaaaaatc
    10321 tataaaaaat ttttgcaaga cggcgtagat gtcgtgacga tggggaatca tacgtgggac
    10381 aaccgcggaa tctttgagtt tgtaaatgaa gctaaaaaga tggttcgtcc ggcaaatttt
    10441 ccggaaggga cgcctggaca agggatggtc tttgtcaaag tgaaccaagt cgaactggca
    10501 gtgatcaaca tgcaggctcg ttcttttatg gtggacttgg atgatccttt tcgtaaaatg
    10561 aatgagctgg tagaagaagc cagaaaacgt acgccgatca tttttgtaga ttttcatgga
    10621 gaaacaacaa gtgaaaaaca agcaatgggc tggtttttag atgggaaagt ttcagcagtg
    10681 gtcggtacac atacacacgt tcagacaaat gatgcgcgaa ttttgccaag aggaacagcg
    10741 tatctgacag acgttgggat gacaggtccc tatgatggta ttttgggtat gcgcagagaa
    10801 cctgtaatcg aaaagtttct gactgcatta ccaaaacgtt ttgaagttgt ggaacaaggc
    10861 cgatcgattt taagcggctg tatcttggaa ttggatgata cgacaggtca tgcaaaagaa
    10921 atccagctga ttcagatcaa cgatgatcga ccgttcatgg agtagaaaga gaggcagttt
    10981 tttgcagcag gagccggaaa ttcaaaaaga agtaaggaaa ctgacaaaac ttttaagaga
    11041 gaatgaaacg atcattcgct acaaagaact tgaagaaaag atccagcaaa accagtattt
    11101 agctgagttg agagaaaaaa tcaaacaagc gcaaaaagat gctgttcatt ttgcccacta
    11161 tgacaaacca gcagcagaaa aagaagcaat caaacaagcg gatcaattca tgcaagaatt
    11221 tgatcagcat ccgcttgttg tagcttatag aaaacagctg ttggaagcag atgatctgct
    11281 tcatcattta acaactatga tacaagaaga aatcaatgga caaatagagg aggaaaaaca
    11341 tgcctcaaaa aactaaaaat actcctatga tggaacaata tctaagcatc aaggcccaat
    11401 accaagatgc ttttttgttc tatcgtttag gagactttta tgaactattt tacgaagatg
    11461 cgatcaaagc gtcacagctt ttggaattaa cgttgacgag cagaaatcga aatgcagaag
    11521 aacctattcc tatgtgcggt gtacctcacc atgcagcaca aggatatatc gatgccttga
    11581 ttgaaaaagg ctataaagta gcgatttgcg agcaggtaga agatccaaag acgactaaag
    11641 ggatggtcaa gcgcgaagtg atccagctag tcactcctgg tacagtcatg gacagcaaag
    11701 gattgtcggc aaaagacaac aattttttaa cagctgtagt tagtcaagga aatgaatttg
    11761 gctttgctta tgccgattta agtacgggag aattgaagac ggctcggttg catgatgaag
    11821 aagctgtttt aaatgaagcc tctgcattgc agacaaaaga actcgtatta ggcagtgaga
    11881 tcactgatac ccttcgtgaa cagctaagcg ggcgtttagg gctagtcttt tctcaacaaa
    11941 atgagatgga agaaaacgcg gaatttagct ttttgacaag cgaactagtc gatccgctgg
    12001 aaaaagaagc aaccggcaaa ctattgactt atttatcggt gacacaaaag cggagtttag
    12061 ctcatatcca aaaagcagta gaataccaac cagatcattt tttgaaaatg gattattatt
    12121 caaaattcaa cctagaatta agccaatcta ttcggactgg gaaaaagcac ggcactcttc
    12181 tatggttgtt agatgaaaca aaaacagcaa tgggtggccg tttactaaaa caatggctag
    12241 accgtccgct tatccaggca aaacaaatta aagcaagaca agaaatggtc gcttctttgc
    12301 tagacagcta ttttgaacgt atcgatttgc agtcagcatt aacgaaagtc tatgatctgg
    12361 aacgcctagc aggtcgagta gctttcggta atgtcaatgg gcgagacttg atccagctga
    12421 aaacttcttt agaacaagtg ccaatgatca gagggttgat tgaaggaatc gatcaaggtc
    12481 aatggcaaag tctgctttca gaaatgcagc cgatggacca tctcgtccaa ttgatcgacc
    12541 aagctatcca agatgatccg ccgttacaaa taacggaagg aaacatcatc aaagatggat
    12601 tcaatgagca gctcgatatg taccgttctg ctatgagaaa cggaaagaaa tggttggcag
    12661 aacttgaagc aaaagagcgt caagaaacag gaatcaagaa cttaaaaatc ggatttaacc
    12721 gcgttttcgg ttattatata gaaattacaa aagcaaatct tggaaatgca gaactggaga
    12781 agtatgaacg aaaacaaact ttagcaaatg cagagcgttt catcacacca gaactaaaag
    12841 aattggagac acaaatcctc gaagcagaag aaaaatctgt tgatttagaa tatcagttgt
    12901 ttttagctgt gagagaagaa gtcaaaaagg ctatccagcc gctacaagta cttgcaaaag
    12961 caatcagtgc agtagatgtg ctacaaagtt ttgcaacgat cagcgaacgc tatcagtatg
    13021 ttcggccaga acttgtcagt gacaagcatc agcttctgat caaagacgga cgacatcctg
    13081 tagtagaaaa agtattagga catcaggaat atatccctaa cagcgtagaa atggcagaag
    13141 acgaaatgat cttgctgatt acaggaccga atatgtctgg taaaagtacg tatatgcgac
    13201 agttggcatt aactgttctg atggcgcaaa tgggctgttt cgttccggca aaacaagcag
    13261 ttttacctat ttttgatcgg atttttaccc gaattggtgc aagtgacgac ttaattgctg
    13321 gacaaagtac ttttatggta gagatgatgg aagccaatca agccctgcgt tatgctacgc
    13381 caaacagttt gattttgttt gacgaactag ggcgaggaac agcaacctat gacggaatgg
    13441 cgttggccca agcaatcatc gaatatatcc atcgtcatgt acaagcaaaa acactctttt
    13501 ctactcatta tcatgaactg acggtacttg agaaagaact tccgcagctt aaaaatgtcc
    13561 atgttggagc cgttgaaaaa gacggagaag ttgtcttctt gcataaaatg atggatggac
    13621 ctgcggataa aagctacggc atacatgtgg caaagatcgc aggattgcct tccaatctac
    13681 tagaacgagc agcagatatc ctgcatacgc tagaaaatgg cgataacgga caccatactg
    13741 ttccagctga agtagaagag aaaactaaaa cagccaaaca ggagcaaata aaaccacctg
    13801 cagaaaaagt aaatcaagac catatacaag accgtgtaaa agaagaaaac caacagcttt
    13861 ctttattcag tgaattatcc gaaaatgaaa cagaagtcat tgaatcactg aagcaaataa
    13921 atttactaga aatgacgcca atggatgcac taaacacatt atatgaatta caaaaacaac
    13981 tgtaggaaga aggcggatat cgatggccaa aatccaagaa ttatctgaac gtttagctaa
    14041 tcagatcgct gcgggagaag tagtggagcg accagcttcc gttgtgaaag aattagtgga
    14101 aaatgcaatt gacgccggca gtactcagat tgatatctta gttgaagaag cgggactgcg
    14161 gacgatccaa gtgattgata acggagaagg cattttggca gacgacgtag agaatgcctt
    14221 caagcggcat gcgacaagta agatccataa tcgcgacgat ctttttcgca tacgaacgct
    14281 gggctttcga ggggaagctt tgccaagtat tgcctctgtt tctgaaatga ctctggaaac
    14341 ggcgaatcag gaagaaaaac aaggcaccta tctgtcgctg aaaggcggag aagttattga
    14401 gcatcgacca gcaccactgc gtcaaggaac gaagattacc gtcagcaatc tgtttttcaa
    14461 tacgccagcc cgcttgaaat acgtgaaaac actgcacaca gaattagcga acatcgggga
    14521 catcgtcaat cgactggcat tgagtcatcc atcgattgct tttcgtttag tacatgatgg
    14581 aaacaaaatg ctggcaactg ctggaaatgg cgatctgaaa caaacaattg cgggaatcta
    14641 cggattatct accgcaaaaa aaatgctgaa aatcgaagga aaagatctag attttgagtt
    14701 atttggctat gtttcattgc ctgaggtgac tcgtgccagt aggaattact tatcgacgat
    14761 catcaatgga cgttttatca agaacttttc cttgaataaa gcaatcgttg caggttatgg
    14821 gtcaaaatta atggtcggtc gtttccctat tgctgtattg gaaatcaaaa tggacccgct
    14881 tttagtagat gtaaatgttc atccgacgaa gcaggaagtc cggctgtcga aggaaaaaga
    14941 actaacagag ctaattagta acgccattca aaaagcattg tctcaagaaa atctgattcc
    15001 aagtgcagcg gataatctac ggtttaagaa aaaagtacct gatcagccta aaacagaaca
    15061 aatcgaaatc gatttgtcct acgcttttga agaagaaaaa cctaaaaagc caagtagttt
    15121 gaattttgat cgagagacag gtaccttttt cgtagaagaa ctaaagaaca catttgttga
    15181 cagaattgtt gataagtctg tggaaaatgt tgaaaactat tctggttttt ctgaagaaaa
    15241 caccacttat ccagaaaaaa cagcgaaaac ggtggataag tttggtgaaa atgttgataa
    15301 ctttgtagaa aaaacagtgg aaaaatctga aaagaaagaa tttcccattg tggataacat
    15361 ggagaaaaca aatgaagaac aagcagataa atccgtagaa gaggatgaaa agatttacga
    15421 ggaagaattg acactccatc ctgaatctga tttcactcaa ggaaacgaca aaacattaga
    15481 acaagccatc aagaaattag aaacggaaaa accagtacaa cgttttcctc agttggaata
    15541 ttttggacag atgcacggaa cgtatctttt tgcccaaagt agcgatgggt tgtacatcat
    15601 tgatcagcat gctgcccaag agcgcattaa gtatgaatat ttccgggaaa aaattgggga
    15661 agtatctgat gatctgcaag aattgcttgt tccatttgtg ttagactatc caaacagtga
    15721 tgctttgaaa ttaaaagagc aaaaacactt gctagaagat gcagggattt atttagaaga
    15781 atttggtcaa aacagcttta ttgtacgggc ccatcctact tggtatccca atggagaaga
    15841 agaatcgatt attcgggaga tgatcgatat gctgctgaca actggaagtg tgagtgtaaa
    15901 aaaattccgt gaagcaacag caatcatgat gagttgcaaa cgatcgatta aagccaacca
    15961 ttatttgaat gaagcacaag cacgtgtgct gctgaaggat ctggcacgat gcgaaaatcc
    16021 tttcaattgt ccccatggcc gtccagtatt gattcacttt accaattcgg atatggaaag
    16081 gatgttcaaa cgaatccaag acccgcatta agtcaaagag aagagggaaa acatgaagat
    16141 tgtattagca tcccaatcac caagaagaaa agagttattg gctcatatcg tttcagaatt
    16201 cgagattgaa cctgctgata tcgatgaaac gccatttcca gacgaacagc ccgtcgatta
    16261 tgttcgtaga atggctgaag aaaaagcacg aactgtctgg gaacaagatg aaaaagaaga
    16321 ccatttagtg attgccagtg acacgactgt tgttctcaat caagagatca tgggtaaacc
    16381 agaagatctg gctgaggctg aattcatgct gaaaaaacta agcggggaaa cacatactgt
    16441 atatacagct gttgttttaa aaacaaagga acgagaagaa cggatactag cagaagcaca
    16501 tgtcactttt tatcctttga cagacgaaga gatcaaacgc tacttggaga cgggggatta
    16561 tgcggataag gcaggggcat acggcatcca aaatgctgcg agtgtattcg tcaaagaaat
    16621 ttctggagat tattacagta tcgtaggatt tccgattggt gctgtcaatc aggcactgaa
    16681 aacattcatt taaacaaaaa agcggactat ctgattttcg attgaaaatc catgatagcc
    16741 cgtttttttt attgaaaaga ttacttatct actctgacta agattttact tcagctttac
    16801 cgactgcttc gcccgggcga acaagtcctg attggtcaag agaaagtcct gcaacttttt
    16861 cattattggt caaagcaaca ataatgtcag ttttcttgcc agcttgttct atttctggca
    16921 aatccatttc agcaatcagg gtctctggtg tgacaaggta tccttctttc acgaatacat
    16981 tgaaaggaac accctttagt tcaacagtat ctaatcccat gtgaacaagt acttccagcc
    17041 cctcattaga aagaatacca atggcatgtt ttgtttcaaa gacactcgtc actttaccgt
    17101 ttactggtgc atagactttg ccgtttgacg gttcgacagc ataaccgtca cccatcattt
    17161 tttgtgagaa aactgggtca tttacttcag ttatttcctt gatggtccct gcagcgactg
    17221 cgaataatgt ttcttttgtt gcaactgccg taccagttgc tgaaacaggt tcgaaagact
    17281 gtggcgttgc tgttgcgcca tttgctgttt gaagttgtac atcagcagca ttttcaggaa
    17341 cagcaggatc aatcttattc aaaattccct gacgtctaaa gatgatcgtc aagccaaatg
    17401 gaatgatgac agcaatgatc attgcgatga tgaaaggtac ccatgtttct gcttggatag
    17461 ctagtattcc tggcaaaccg cctactccga tcgcgttagc tcgaacaccc atcaagttag
    17521 caaacatgcc agctaatcca gagccgacca ttgcagcgac gaaaggatag acgtatttca
    17581 aattgattcc gaacatagca ggttcagtta cgcctaagta acaagaaatc attgctggaa
    17641 tcgagatttg ttcttctttt ttattcccac gatgtaagaa gatgatggct aagactgcag
    17701 agccttgggc gatatttgat aaagcaatca tcggccataa attcgttgat ccgaaatctg
    17761 caatcagctg catatcgatg gcattggtca tatggtggag accagtaatc actaaaggag
    17821 catacaggaa tccgaatacg gcactaaaca accagctgat agaagacgtc aatccagtat
    17881 ttacgatatt tgagatccag cttccgactg tccagccgat aggacctaaa ataacgtgtg
    17941 cagctaaaac agttggtaac aatgaaaaca atggaacaaa aatcatcgag atcgactgag
    18001 ggatatattt gcggaagaag atttctagat acgctaacat aaaccctgcc agcatggctg
    18061 gaataacttg cgcttgataa ccaatcatct ggacttgagc aaaaccaaaa tcccaaaatg
    18121 gaatatctgc tgcggctgtg gaagctacac tataagcatt taataattgc ggagaaacta
    18181 acgtaattcc taaaacgatt cctaatattt gtgtagtacc catctttttt gcaatactcc
    18241 aagtgatacc tactggaaga aaatggaaaa ttgcttcacc gggtaaccac aaaaaggcat
    18301 tgacaccatt ccaaaacttc gagtgttcaa cgattgttcc accaagactt tcaaactgga
    18361 taccttcaag tacatttctg aatccaagga tcaatccacc gacaatgatt gcaggaatca
    18421 gtggtgtaaa gatttctgct aaaacggcaa tagcacgttg aacgggatga aggttttgtt
    18481 ttgcagctgc ttttccttgt tctttggaaa ccccttcaac tccagatact gcaacgaagt
    18541 cgttataaaa agtagaaaca tcgtttccta tgatgacttg gaactgtcct gcattcgtga
    18601 acatcccttt tacgctaggt atatcttcga tcgctttttc gtccgctttt cctggatcat
    18661 tcaggacaaa acgcatacga gtcgcacaat gactaactgc agcaatattt tcttttccgc
    18721 cgatatcatg taacagcttc tcggcatctg cttgatattt tgccatatca ataaacccct
    18781 ttctcttaac ttgtctatgc aagttgtttc ttcatttact atactttttt tcggagagaa
    18841 ttgcaagcgt tttattaaaa agtacgagag aaaatgatat cgttttaaaa agcttaaaat
    18901 aagcttttct taaaaatagg aacattgaaa tacaaaaaga gctgtagtat agtgaagaaa
    18961 tgatggaaga acgagtgagg gaaaaaacat gaataagttc aatgaaattt ttttagattt
    19021 ggaacagaaa attttagcga aagaatatcc tccgcattcg cttttaccca gtgagaacca
    19081 attgatcaaa atttatggtg tttcacgaga aacaattaga aaagcgctga atcttttgac
    19141 tacggaaggt tatattcaaa aaaaacaagg aaaaggttcg atcgtactgg atcggaatcg
    19201 cttcgacttt cctatttctg gattgactag ctataaagag ctacaggaga cccaacgtat
    19261 tccaagtgaa acgatcatcc atacgttaga agaaacagaa gtgacaaaag cattgaacga
    19321 gatcactggc tgggagatcg gtgcgcctgt ttggcatttg atacgcgaaa gaaaaatcaa
    19381 tggcgaagta gttatattag acaccgatta tctattaaag gaaatcgttc ctattttaac
    19441 tccagcacaa gcacaaggat cgatctatga atattttgaa aatgagcttt ctttgacgat
    19501 tgcttacgcg caaaaggaga tcacagtaga agaagtaacg gaaatcatga aaacagcgat
    19561 ggatcttcac gatgacactt atgcagtagt agtcagaagc ctcgttcatc tggaagatac
    19621 ccgctgtttc gagtattctg agtcggttca ccgattagat aaattccgtt tcgttgagtt
    19681 tgcccgacgc cgaaaagtgt aaatagttca aaagaaagca aattgttggc atctgtccac
    19741 tttggcgcta tgataggatc acaaaggggg catttttaat ggataaacca ataagaccag
    19801 aaaaagaaga actgaaagaa aagttatcac ccatggctta tgcagtcaca caagaaaatg
    19861 caacagaacg accatttagc gggaaatatg atgattttta cgaaaagggg atctatgtag
    19921 atatcgtcag cggtgaaccg ttattcagct ctgcagaaaa atatgatgca ggttgtggct
    19981 ggccatcatt cagcaaacca ataacgaaaa gacaagtgcg cgaaaaagcc gatttttctc
    20041 atggcatgca tcgaatagaa gttcgaagca aagaagcaga ttctcatctc ggacatgtgt
    20101 ttaccgatgg accagtagat caaggtggtc tacgttactg catcaattct gcctcactga
    20161 aattcattcc atatgaaaag atggatgaat taggttatgg ggaattcaaa tcacttgtag
    20221 aataatggaa atacacaaag agcggtacaa tacggggatt tactcgatcg tccgctcttt
    20281 ttttattcaa gcgattgcta tttgaaagaa acgctcccta gactcctttt ttcacaaggt
    20341 gaaatgtgct acaataaagg gaacgtatgt gtgcaaaaga aagaggacta gaatatgtat
    20401 gaatatctga caggtgtggt tacctttatc aacccctatt acatagtagt agaaaccaat
    20461 ggtatcggct accagatcgc actaggaaat ccttaccgct acagtagcaa actaaatcaa
    20521 aaaataaaaa tttatgtcca tcaggtaata cgtgaagatg cgcatcttct gtatggattt
    20581 gattcgctag aagaaaagca actgtttttg cgattagtaa gtgtttcagg aatcggtccg
    20641 aaaagtgccc ttgcaatcat ggcaagcgaa gaccactccg gtttgattca agcagtagaa
    20701 tccagtgatg tcacttattt gacgaaattt ccaggagtag ggaaaaagac agcacaacaa
    20761 atgatcttgg atctaaaagg aaaattcggt gagctgagta tcgatacacc ttttagtcta
    20821 tttgatgaag caaacacaaa agacgctaca gctctatctg aagcaatgga agctctctcg
    20881 gctcttggtt acagtgatcg agaaatcaaa cgtgtagaaa aacaattaaa agaagtagat
    20941 aatcagacaa cggatgagta tctacgccaa gcactcaagc taatgatgaa aaaataagga
    21001 ggagaccaaa tgacagatga atacttgtta tcaccagaat ctggcgaaaa tgaactgagt
    21061 ttggaaaaat ctcttcgacc acagctgctt tctcaatata tcggacagag aaaagtcaaa
    21121 caagaacttc agatttacat cgaagctgcc agaaatcggg aagaaccttt agatcatgtt
    21181 ctgttgtacg ggcctcctgg attaggaaaa acgacgatgg ccatggtcat tgctaatgag
    21241 atgaccgtaa atattcgtac gacgagtggc cctgctatcg aacgtccggg tgacttagta
    21301 gcaatcttga atgaactgga agcaggggat gtcctgttta tcgatgagat ccatcgatta
    21361 cctagagtgg tagaagagat gctttattca gctatggaag atttctatgt agatatcatg
    21421 gtaggacaag ggccaaccgc acatccggtt catttccctc ttcctccctt tactttgatt
    21481 ggcgcgacga cacgggcagg tatgctttct gcacctttac gagatcgatt tggcatcatt
    21541 tctcatatgg aatattatga cgaacaggat ctgcaggaga tcgttttacg ttcttcggat
    21601 atttttcaaa cagagatcat tgaagaaggg gcattggaaa ttgctcgccg atcacgagga
    21661 acgcctagaa tcgctaatcg cttgctcaaa cgggttcgtg attttgcgca agtccaagga
    21721 gacgggaaaa tcgatcgtcc tatagcggat caggcactta ctttgctaca ggtcgatcag
    21781 gctggattgg attatgttga ccagaaattg ttgcgaacaa tgatcgagtt atatggcggc
    21841 ggaccagtag ggttaagtac gctttcagta aacataggag aagagcgtga gacggtagaa
    21901 gatatgtatg aaccgtatct gatccaaaaa ggatttttaa agcgtacacc tagagggcga
    21961 attgctactg cttatgccta tgaacatttt ggttacgact atcatatatg atttttgctt
    22021 ttagattgga ggaaaatgaa tgagtgatac aacacgtacg aaacaattga tcaaaaacgt
    22081 attgattgat ttgtgccaaa cgcaatcttt tcgaaaaatc agcgtccaaa aaatcgcaaa
    22141 cgaagcaaaa atcaatcgac aaacatttta ttatcatttt acagataaat acgatttgct
    22201 tcgttgggtc tattgtgagg atgcattgca ttatttagat ccacaatgtc tttcacttga
    22261 taattgggaa gagcaagcat tgaaaatgct gaaagcaatt gctgagaaga aagaatttta
    22321 tgcaaatact gtgctttctg acccagaaat tttacaaaaa gagttttctc aactgatcca
    22381 aaaatcattt atcaagattt ttgaacaaat ggatgaagaa agacaacttt ctgcaagaga
    22441 caaactattt tatgctcgtt ttttttctta cggttgcagt ggtgtattag tcaattggat
    22501 cacgaatgaa tatgaagaaa caccgatgga aatcgctatg cagcttttcc gtttagccaa
    22561 ggatacggaa ttatattctt atcgtcttta tgcaaaagaa gagttggaag aacagtaata
    22621 gaaaaatttt ttgttggaaa agcaataatc gctaaaagga gatggggaaa tgacaaaaat
    22681 tttatttgtt tgtttgggaa atatttgccg ttcaccgatg gcagaaggct tgctgagaaa
    22741 acgaatagca ttagaaggaa gggaagcaga attttttgtg gattctgctg ctacaagcac
    22801 gtatgaagtt ggaaagacac cgcaccctgg aacgaaaaaa atattaaacc aagaaaaagt
    22861 agatatgacg aatatggttg ctcggcaaat cacaccccac gattttgaaa cttttgattg
    22921 gatcattggc atggatcaag aaaatgtaga agaactgaaa cgaagagcac cacgctcagc
    22981 gcaggggaaa atccatttgt ttttaagtag cattcctgga aaagaaaaag aaaatgttcc
    23041 cgacccatac tataccaata actttgatga gacatatcac ttgatcacag aaggcctgaa
    23101 tcattggtgg aatatttgga ttgatgaaaa taaaataaca aacaatttgt aaagttaacg
    23161 ctttcttttt aaggcgatgt tagagcattt ccttgttttt ttgtaaaatg ttaggtaaaa
    23221 taaaattaat taagtaacac tttcttagct aaaggaggag tgcattcgat gaacgaagag
    23281 acagtatcgc caatgaagaa gcttacgaag ttagttactc atacttcgta tcaacttttt
    23341 gattcttttg taaatgacac tgaagaacat gctaagaatg tacgattgca aggaaagttt
    23401 attcttgagc tgcaaaaagc tgctactgaa catagtctag ttatcttaca agtaagagca
    23461 aatcgtttga gtagcaagta tgaaacgatc tcaggctggg tggcgacaaa aacggtcgaa
    23521 aaagaccgag tgatgatccg cctacaaaac gaagagcagc agatccggat ggttcctgta
    23581 gaacaaattg aaaaaataac aatcctttca ccagcagggg aacaggaacg gttatcaaaa
    23641 taatttatac cagatagaaa agcacgtatg agcttgtacc atcaatcatt actaatcaaa
    23701 aataaccgaa tgctttaagc tgatatcagt aaaacggaac gttttaggat gagtatcaca
    23761 gcatgacagc attgggttga ttagttgaat cgattggatg gtacaagttt ttttgataaa
    23821 aaaaggtcct gctagccggg gagctagcag gataaggagt taaaaatgaa aaagtgttgt
    23881 tagggttgtt tgttggtatg cttatataat agtcactagt tgtgaatatt ttgtgacgaa
    23941 agttttcgaa agttgttaag tttttttgcg aaaactattt ttatatcata tatatgtcta
    24001 aaatctatat tataaaagaa atgttgtttg atcagcttct cttcggtatt agttcaaaac
    24061 aaaacggcaa agtatatgga gctactttgc cgttttgttt ctctaatact caggtcagtt
    24121 tatagacgct caataaacgg tgttcaaaag ccagtttctc ggtattaagt caaatttatg
    24181 caaaaatgtg agaagccatt ttcaaaaaat atttcttaat actcgaaact gagcggcttt
    24241 ttcacgacct ctcaataaac agtgttcaag agacagccta tcggcaataa ggctgaacgt
    24301 ctctttcaca acctctttta tcaatctgct acttcttctt tcttagcgaa actccaaatc
    24361 agtccgataa caaaaccgat aactgctggt acgatccacc ccatccccaa tttgaaaaac
    24421 ggcaagtagc tttccgcaac atataaaaga ttttgaacaa taggtgtttg tttgatagtt
    24481 tctgggcaag cgttcaaccc atcaaaaatc gaagcaatca atgtaaaata agtagtcatt
    24541 cgataaacag ccgtacgctg cttaaacaaa ggaccaacta acactagtaa aatcagtgtc
    24601 attgctaatg gatagatgaa catcaaaaca ggcaacgaaa attgaatgat attcgtcaag
    24661 cctacgtttg ccgctaaaca aggaaggata ctagcaagtg caacaaagaa tgcgtaattt
    24721 ctttgcggaa acaaatctgt gaatgtttct gcaaaagctg taatcaaacc aataccggtc
    24781 ttcaagcaag ctaatataac gataaatgct aataagatac tgccataagt acctaaataa
    24841 tgatcagcaa tctgagccag cgtgatcccg ccattttcac ttagagcaaa gctacctaag
    24901 ctcatagcac ctaaataagc gagcaaagta taaatgattc ccatcaatat aatgctgatc
    24961 gcaccagatt tgatcgtgtc tttagcgatt tcagcaggtt ttgttacacc catgttttgg
    25021 attgttgtga caatgatgat cccaaatgct aaagcagcaa gcgcatctaa tgtgttatat
    25081 ccttgagtaa accctgtgaa aaaagcatgc tcttgataat ttggctgaat aggcgcttga
    25141 tttacagaac ctagtggatt caaaaaagca aggagaagca aagcacctaa taaaaccaaa
    25201 aatgcaggat tcaggaactt tccaacataa tctaatatct ttgtcggttt tcttgaaaac
    25261 caccaagcca gcacaaaaaa tacaatactg aaaattgcta gatatagcgt ctgtctgcta
    25321 ggatcgataa atggtgctaa accaatctcg aaagatgtgg ttgcaaggcg aggtaaagca
    25381 aaaaacggtc cgataactaa gtaaagcaag atggtaaaaa ccatcgcata tgtttgattt
    25441 atccgttttg ctaattcata tacacctgag cttttcgata caccaatggc aatcacccct
    25501 aagaagggaa gaccgatacc ggtaaccaag aaacctagat tcgcccaaaa ggtagcagcg
    25561 cctgcttctt gacccaaatg aacgggaaag ataagatttc cagcaccaaa aaatagaccg
    25621 aaaagcatgg agcctataaa aagatagtct ttaaatgata atttttttgt catacataac
    25681 tcctttacgg tttttttaaa agtaacaaaa tttcgaaaaa tatgcaacaa ttttcagaaa
    25741 atttcctata taaaacgacg gttatttaat aacgtaagtg ataaaataaa agcgctgaat
    25801 catctgagga ggaatcatcg tgacaaagtc gagattagaa gcatttactg atggagtggt
    25861 agccattgtt ttgagagttc ttgtattaga gatcaagatc cctaatacat ctagctttca
    25921 ttctttatgg gtcatccgaa atacattatt agcttatacc atcagcttta tatttgttgc
    25981 ggtgatctgg gtgaaccatc atcgtatatt tcagatggca gaacgaatta attaccgagt
    26041 ggtctggtcg aatatctttt tgctcttttg gctgacttta tgtccttctg ttactagttg
    26101 ggtcgggcgt aaccctgaag ctttttggcc tgagatggct tatgtcttag tatacacgat
    26161 gtggagtttt tcttttggta tcttgatgag gcaaatcatg aaagcaaata aaccagattc
    26221 ccatgtcgtg agagttttgt caaaggaccg tcgaagtctt atctccatga tgattaactt
    26281 atgcttgatt gccggtgtct tttttgtacc gttgatcggg ttattcggac gtttttttgt
    26341 atctggtatt tggatttttt cctataaaaa agcagatgcc tattatcagc ggttgtttcc
    26401 ggggaaatag aaaagaggct gggacagaag cttttaactc caagaaataa gccgaaattt
    26461 cacaaaaatt taagaagcaa ttttcgtaaa ttccttctta tttctcgaag ctgaccactt
    26521 ctgtcacaac tactttctta cttcttacgt tcatatttca tatgatagat tagtaataga
    26581 agcaagaacc acacaggtgt tagtaataaa gcgattctcg tatcttttcc taggaacaag
    26641 acgacacaca caaaggccat gtaaagaaat accagataat tactaaatgg gtacaacggc
    26701 attttaaatg aatgagcttt tttgccgagg atacttttac gatatttcat gtgcgtgtag
    26761 acaagcatac tccagataaa caagaaacaa gtagtggcaa tactgcttac tagtgtgaat
    26821 acttcactag gcatcacgta attcaagata acagaaatga aaatgacgat agtagaaaat
    26881 gtcaaagcat tccctggcac atgatgtgtt gttaactttt tgaatttttt aggggcactg
    26941 ccttcttgcg ctaaagaacg aagcatacgt cctgtactat atattgcact gttgcaggca
    27001 gaagctgctg cacttaatac gactaaattg atcaaggaag cagcaatcgt gatcccaatt
    27061 tccgaaaata cttctacaaa tggactgtta tctggattca agctgttcca tggatagatt
    27121 gccataatga caaaaagaga tcctaagtaa aataagatga tcctgatagg aatattattg
    27181 atagcttccg gcaacacttt ttcaggattt tctgtttctc cagcgactaa tccaactaat
    27241 tctatcccag caaaagcaaa ggttaccatt tggaaagcta agataaatcc ttttgcacca
    27301 gttggaaaga atccgccatg agaccataga ttcgtgatac tggctgtgcc aatatctgtc
    27361 gtatagtggg tgaggatcat ataagctcct acgaggataa aggcaatgat cgctacaact
    27421 ttgatcaatg caaaccaaaa ctctaactca ccaaacaatg agacagccac catattcaag
    27481 caaagaagaa gacctaatgc aatcagttca ggaagccact gaggaacacc aggcgcccag
    27541 tagcgtacat acattccgat ggctgttaaa tcggccattg caatcgatac ccagcagaac
    27601 caatacgtcc agccagtgat gaaagccgct tttttgccaa gatattctgc aacaaaatcc
    27661 agaaaagaat ggttattggt gtttgataac agaagttcgc ctaacgcacg cataatgaaa
    27721 aaacagatgc atccagtaat aagataggcg agcaatatcg aagggccagc tagctggata
    27781 gactttccgg aacctaaaaa caaccccgtt ccaatcgctc cgccaataga aatcagttgg
    27841 acatgtctat ttttcagtcc acgctgcagt tcttgctctt cattcatttt taaatcatcc
    27901 taactaaaaa agtttaatta cttccatatt aaaagagttt tctgaaaaac acaactaaga
    27961 ttccaaaatt ttcagaaatt taccttttat gagtaattca taaaaaagaa ctgcggtaaa
    28021 caccgcagct ctttgtccca atcagctatt gatcctaagc atttaaaata cgtgaaagga
    28081 attcttttgt acgttcttct ttcggatgga caaagatatc ttctggtgta ccttcttctg
    28141 caatcacacc tttatccata aaaatgacac ggtcagatac atcttttgca aattccatct
    28201 catgcgtcac gataagcatt gtcaagccag tatgtgctaa gtctttgatc gtattaagca
    28261 cttcgccaac catttcaggg tctaacgccg aagtaggttc gtcgaatagc aatacatctg
    28321 gattcattga taatgcccga gcaattgcga cacgctgttt ctgtccacca gataattgtg
    28381 ctggtttggc ttcgatataa cgatccatac caactttttc cagattttcg attgctactt
    28441 ttttggcagt ttctttatct cgtttcagga cagttgtttg accagacata cagttttcca
    28501 agacattcat attgttgaac aggttgaagg attggaaaac catacctaga ttcgttcgat
    28561 atttaggtaa attatattta ggagctaata cattttctcc attataaatg atctcgccgc
    28621 ctgttggttt ttccagtaaa ttgatacagc gcaagaaagt agatttacca gaaccggaag
    28681 aaccgatgat cgtcacaact tcacctttat tgactgttac gctgatatct ttcaatactt
    28741 cattttcacc gaagcttttt cttagatgtt tgatttcaat aattttttca ttcatgattc
    28801 ttcctcctta ttcctgcaaa tttgaattgt cgatttcttc aagcttggta taagctgaag
    28861 gaccatcgat tttcttttct accgcccgca ggattctggt gatcgtaaag gtcatgacta
    28921 agtagatgat cccgacgatc gtaaatgttt ggaagaattg gaaattcgca ccggaagcag
    28981 agtttccttg gaagaacaaa tcagctacgc cgattacact taaaactgct gtatctttga
    29041 tattgataac aaactcattt cctgttgctg gcaagatatt ccgtaaaact tgtggaataa
    29101 caacttttct catcgtttgg ccatgggtca taccaatggc ttgcgcagct tcgaactgcc
    29161 cttcatctac tgcaaaaatc cccccacgaa cgatctctgt catgtaagct ccagtattga
    29221 gagagacaat aaacaaagcg gcaatcgttc ggttcaatga aatgccaaaa gcgagtgcca
    29281 gaccgtagaa gatgaccatt gcttgtacca tcatcggtgt tccgcggaat acttcgacat
    29341 agatagaaag aagtgcattt ccgatttttt ggaagaagcg agtcagtgca tttcctgatt
    29401 caggtaccga acggaagaca ccaatcagta atccaagtgt agtaccgaca actgtcccga
    29461 tcaatgcgat gaacaaggta agtccagcac ctcgtaagaa catattgccg tattgtttcc
    29521 aaatcgtagt gaaatcagaa atgatgccag gtttttcttc actatcagtt gcagcaggct
    29581 gatctttgac tgcttggtcc ataattttgg ttcgttcatc gttagagatg ccagaaagaa
    29641 tctggtttac tttttggata tccgggtcgc ctttacgcat accaacagaa atctgaacat
    29701 cttccgggtt tgtctggaag ccatctgcac cgctgaattc aagcatttcc aagtctttgt
    29761 tcacactagt tgccgttaca ccttccggac gttcgctgac ataaccatcg atcattccag
    29821 aagctaaagc tgttctcatt gctgagaaat tatccattgc ttgttgtttc tgcacatctg
    29881 gtatctgatc gatcacagta tagtggaatg tatttaactg agcagtaatc tttgctccag
    29941 aaagatctgc taaacttttt gcttttgcat atttgctatc tttttgaact actactacta
    30001 attgtgattc gtaataagga tcggtaaaat caatttcttt tcgtcgttca gcagtagggc
    30061 tcattccggc gatgatcgcg tcgatcttgc ctgattgaag ggcaggagcc agaccatccc
    30121 atttcgtttg gacgatgact aattttttcc ctaaaccatc tgctactttt ttagcgattt
    30181 gaacatcata accgccggcg taactatttt ggccttgaat tggcaaggca ttattctcat
    30241 ctgtttgttg agaccagttg aagggtgcat acccagcctc catacctacg cgaaactcat
    30301 cgctcggtgt tttttcatct gcatttgcca gagatcccac actgaatagt gagaagacaa
    30361 aagcaaatag tacggcaact gaaaccatat tttttttcat ttttttctcc ctttctgatt
    30421 atatgataca cgagcggtct agatgaaatg cggtaaagct ttatctgaaa aaataaaaag
    30481 cgaccgattt atagatataa aaaggccgct aaaattccat aaggtgataa acctctcaca
    30541 aaacatagcg caacttagca tcactgctaa gacagtccgc aagctcttaa cttgcgtccc
    30601 aacatacact ccaagcaaaa aagtgcacat ttcggcgatc ctccctattc ctgcttctcc
    30661 atgtttgctt cactccacag ggttaatgat tctcgcgacc tctacctcat tgattgaggc
    30721 atctcgtatt cagttattta tacaaaaaaa gtttacgata ctcgagaggc tttgtcaatc
    30781 tataactgct gttttttgaa aaaatactga taaatataga aatctttact tttaccgaaa
    30841 aaacaatcaa aatgtgaatc atctatccat tgcaaacgat agtataatta tacacaaaaa
    30901 tgtatgtaat acaataaaaa aagaactaga acagaaaatt tcctgtgtcc agttccatct
    30961 cgatcattta agctgccgct ttctttggct cacgtcctag gatcgcttgc aaaagaacaa
    31021 cgacgccata aatggctaat attagataaa tcaaacttga gtattgggtg attccccagc
    31081 cccatgttcg atatacggct aatgtaatgg ctaaaacaat tgcggcaatc acgttgaata
    31141 aagcaaaagc ccaaagattt ccattttctt tgcgtacaag ataaaaaaca gagaaataaa
    31201 cgcttaacgc taaccaggcg atacaagcca gaataacgcc ccaataaata aagaatggta
    31261 acatatcgat tcacctcact tttatacagc agtattgtaa cacgttttca ttcttttgtc
    31321 agtgaacatt gtgataaatt gttcaaatgc ccataacact tttttattcc catttttctg
    31381 acttttcaac aataaatttg ttcgagtatg ggctataatg gaaagtgagg tgacaaaaca
    31441 tgaaacaatt aacagtagac gaagcagcaa aagagatatt agcttatcag caaattacat
    31501 ttttgaccgg tgcaggggta tccacacctt ctggagtgcc agactatcga tctttgtcag
    31561 gaatctacca cgggatggag cagccggaat acttgctgag tcatcaagca atggtgaatg
    31621 aaccggataa attctatcaa tttgtcaaaa agatttatca tcctgaagca aaacccaata
    31681 tcattcattt ggaaatggca catctagcga aagaaaaaaa tgtttgggtt gtttcccaaa
    31741 acatcgacgg tttgcacgca aaggctaaaa gcccgcaact cgtcaatttt catggaagtt
    31801 tgtatcaatg tcattgtcga aaatgtcaac aaacggtaga ctggaaagaa tatcttcaca
    31861 gcgacaaaca tcaagcatgc ggaggacaaa tacgcccaga catcgtgtta tatggtgaag
    31921 ggtttcaaga tggagtcatg gagcaggcgg cttatgccgt cagccaagca gagctgatcg
    31981 tcattgttgg aacgagtttt caagttcatc cgttttgtga tctggtacag tttcgccagc
    32041 cgtcagccaa aatcttagta atcaatcaaa cgcctgttta tttagcggag ccttatacgt
    32101 ttattcaaga agacggagcg gttgttttca aaaaaataca agaattagga gcagattatg
    32161 actgaattca aaaccgaaaa agaaaaaatg attgcaggcg agctttattt tgccaatgat
    32221 cctgagctgg ttgccgaccg tatgtttgca agaagtcaaa gccaaatcat caaccaagca
    32281 gaatcagcgg aactgcgatc ccaattgttg aaagagactt ttggccgtac agggaaaaaa
    32341 atctatatgg agccagttat caattttgat tacggatata atatatttgt tggggagaat
    32401 ttttatgcaa atttcaattg tacattttta gatgtcagca cgattgaaat cggtgataac
    32461 tgcatgttcg ctccgaatgt ccagctttac acggcgacgc atccactaca tccagtgaaa
    32521 cgtaacagcg gtctggagta cgcaaaaccc attaaaatcg gtaataatgt ttggttaggc
    32581 ggaggagtta tcgtcacgcc gggtgtcact ctaggggaca acgtagtagt cggcgcaggt
    32641 tcggttgtga ccaaatcttt tcccgacaat gtagtgattg caggaaatcc tgctcgtatc
    32701 atcaagacag tagaggaaga actaacagaa gagtctcttg aaacattacg tcataagatc
    32761 gatatgatcg atcgagagtt gattgcttta ttagaaaaaa gaatggatac ggtaactaaa
    32821 ataggacaag tgaaaaaaga agaaaaccaa gcagtttatg atgcaaaaag agagcagcaa
    32881 gtattggata aagttgttag tctgctgaaa aataagggat ataaggaaac aataacagat
    32941 acgtatgttg atttgatgaa acactcgcgc gaataccaaa acaaaatgaa ggaggaataa
    33001 gtgtgatcga aggagagaca cggcgaaaac aaatcatgca aaccttggca aatacagaaa
    33061 aaccagtgag tgctagcaag tttgcgaaaa cttttggagt cagccgtcag atcattgtag
    33121 gcgatattgc tttactacga gctgcaggta aaagtatcgt ggcaacagca agaggttata
    33181 tcctcgaaac agaagaaacc aatactgggc acatcagtaa aatcgctgtc cagcatggaa
    33241 aagatcaaac agaagaagaa ctgagactaa tcgttgaaaa tggtggtgaa atcattgatg
    33301 taattgtgga acatcccctt tacggtgagt tagcaggaac tttgcacatc aaaacgccac
    33361 aagatatcga ttcctttatg agacgttatc aaaaaagtaa tgctactttg ttatccgaat
    33421 taacaggcgg catccatctg catacgatcc gctacccaga aaagcatatg ctgaaaaaca
    33481 tcaaacaaaa actagctgaa gcaggaatcc tctacgaagg ataacatcgt agctgctact
    33541 ttttatccta gaaactattc tatcatctct gagataaaca cccatatgga agaaataaga
    33601 aaaaataaga aaagatgcgt atccaataca cagaattttt tggaattgcg atttctagat
    33661 gtctatctaa atttcgatta gtggagaaac ataaaaaagg gaagtgcagc ttttgagcac
    33721 tccccttttt tataaagctt tcttgtagca tagtatcgaa ggaacctgcc aattctctac
    33781 tcgatcacca atacgccttc ttttagtgca aatggatttt gatcgttgat gtggtcatag
    33841 aacatcacac cgttgatatg gtcgatttca tgttgaacga cgatcgattc ataattcttc
    33901 aaacgaatct tgtgtttttc accattcata tcataataag acacagtgat tttggcatgg
    33961 cgaacaacat atcctggtac ttctcgatca acagataaac agccttcacc ttcccctaaa
    34021 caagcatctt gaacagaatg gctcaaaatt ttaggattat acataacagt acttagagaa
    34081 ggtccatctg cctctgggtc aggacttggt acgtgaacag caatgattct tttagaaata
    34141 tctaattgag gagcagctaa gccaacaccg cctcgtaagt gtagttcttc agctttgatt
    34201 ggatcttggc tgttttttaa gaactcaagc atctcttttc ctaaagagat atcttcttcg
    34261 cttaatggaa gagaaacttc ttttgcaact tcgcgtaagg tcggatttcc ttcacgaatg
    34321 atatcatcca ttgtaatcat ggaatatgta cccctttctt aaaagactgt gtttcaagtc
    34381 tattttcgca ttatagttta ccacaaaaat ggaaaagtag tagtaagatt gtttgaaaac
    34441 taccttttct taatgattaa ctacttgatt ttcaacccgg tttcaggtaa agtatagctg
    34501 tgtgctctag gcacatgacc tgccacttgg ttaagcgaat caccaacgtt ttactcagtc
    34561 gcaggggaat aatcgaaaga ggtgaaagaa atggcaattt caaaagaacg caagaatgaa
    34621 atcatcaacg aatacgctcg ccatgaaggg gatactggtt ctccagaagt acaaatcgct
    34681 gtattaactg aagaaatcaa ccacttgaat gaacatgcac gcgtgcacaa aaaagaccat
    34741 cattcatacc gcggcctaat gaaaaaagtt ggtcaccgcc gtaacttgtt agcttactta
    34801 cgtaaaactg acgttcaacg ttaccgcgaa ttgatcaaac gtttaggctt gcgtcgttaa
    34861 ttttagtcaa aaagagtggg attcacatga atctcacttt ttttactcgt atatcaatta
    34921 agtaaaattc cgtgatcaag ctagatggac tcctactaac caaatgaaag ctgctcaaaa
    34981 gacggctttc ggcagttttc atttgtttag tagcgtcttg ctttcacgag aaggaaaagg
    35041 agaagttatg tcagaaaaac aagtattcaa aacgacatgg ggcggccgtc ccttacaaat
    35101 cgaagtcggt cagctagcaa aacaagcaaa cggagccgtt ttagtccgtt atggagatac
    35161 agtagtctta agtgcagctg tggcctctaa agaagcaaaa gatacagact ttttcccatt
    35221 aacgatcaat tatgaagaaa aaatgtatgc agcagggaaa atccctggag gtttcatcaa
    35281 acgagaaggc cgtccaagta cggaagcaac attgactgcc cgtttgatcg accgtccaat
    35341 tcgcccgatg tttgcagaag gcttccgtaa tgaagtccaa gtaacgaaca tcgtgatgag
    35401 tgtagaaaca gactgctcgc cagctatggc agcaatgcta ggttcttctt tagcactatc
    35461 aatctcagat atcccatttg acggcccaat cgcaggcgta gaagttggtc gtgtaaatgg
    35521 cgagtacgtc ttgaatccaa cagtagaaca agcagaacaa acagatatcg aattgactgt
    35581 tgccggaacg aaacaagcca tcaatatggt tgaaagcggc gcgaaagaag tttcagaaga
    35641 agacatgctt ggtgcattgc tgtttggttt tgacgctatc aaagagttag ttgctttcca
    35701 agaagaaatc gttcaagcag tagggaaaga aaaaatggaa gtgactttac tgcaagttga
    35761 tgaagtattg aaaaaagaaa tctttgatgc ttcttacgct acaatgaaag cagcagtaat
    35821 gactgaagaa aaacttgctc gcgaagataa tatcgagcag gtgaagatcg atatccgcga
    35881 agcttatgct gagaaatttg caggtcatga agatgaagat cacttgttga aagaagttaa
    35941 acaaatcaca gaagatcttg aaaaagatgt cgtacgtgaa ttgatcacca tcgataagat
    36001 ccgtcctgac gggcgtaaat tggatgaaat ccgtccattg tcatcagagg tcagcttatt
    36061 gccacgtgta catggatcag gtttgttcac acgaggacaa acacaagctt tatcagcttg
    36121 tacactcgca cctctaggag agcaccaaat cattgatggt ttaggggtgg aagtcagcaa
    36181 acgattcatc caccactaca atttccctca attttctgtt ggttctacag gacgtgcagg
    36241 ctctccaggg cgtcgggaaa tcggacatgg cgcattaggt gaacgtgcgt tagcacaagt
    36301 aatcccttca gaagaagaat ttccttatac gatccgttta gtagcagaag tattggaatc
    36361 aaatggttct tcttcccaag caagtatctg tgctggcaca ttagcattga tggatgcagg
    36421 tgtaccaatc aaagcgccag tcgccggtat tgcgatggga cttgtttctg atggtgaaaa
    36481 ctatacgatc ctaactgata tccaagggct ggaagatcat cttggcgata tggactttaa
    36541 agttgccggt acaaaagatg gtatcactgc tttacagatg gatatcaaga ttcaaggaat
    36601 cacagaacaa atcctaactg aagcattaac tcaagcaaaa caagcacgta tggaaatctt
    36661 agaagaattg acttcaacga ttgctgctcc acgtgaagaa ttaagccaat atgcaccaaa
    36721 aatcgaaatg atccagatcg aacctgctaa aatcaaagat gttatcggta aaggcggcga
    36781 tacgatcaac ggaatcatcg atgaaactgg tgtgaaaatc gatatcgatc aagatggaaa
    36841 agtaagtatt gcttcagctg atgcagaaat gatcaaaaaa gctatcaaga tcatcgaaga
    36901 tttgaccaaa gaagtcaaag ttggtgaagt ttatctaggg aaagttgtcc gtatcgaaaa
    36961 attcggtgca ttcgtcaact tgatcaaagg aaaagatgga cttgtccata tttcccaatt
    37021 agcaaacgat cgtgtcaata aagtcgaaga tgttgttaaa ctaggtgatg agatccttgt
    37081 gaaagtaact gaaatcgata aacaaggacg cgtaaatctg tcaagaaaag caatgttgaa
    37141 cgaagatggt tcaacgaaaa atgaagaaaa ataatttaat tcataggaat aatgtacttg
    37201 tgatcgtgaa ggtgacaggg ggacaataat tattcgaaaa aaggggactg tgacaaaagc
    37261 cgtgtcacag tccttttttt tcgaataaac ggtgttcaaa agcttgcatc tgcggtaata
    37321 agtccagcca agttaaaaac attaggagct gtttttattt ggctgttctt attacttgat
    37381 gcagaacagc ttttttacaa cctctccaat aaacgatgtt caatcagctg tccctcgata
    37441 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gtttgtttct ctaatactca
    37501 ggtcaaaccg ctgatttcac aacctctgat tcacggtgtt ccagaagcag cttctacgga
    37561 aataagctga acacttttgt tacaacttct ttttttctgg agtttcttgg cgcatatttt
    37621 tggagtcctt ctgaataaag cgtaaaataa aagtgagaaa tatggataaa ggagggaaag
    37681 gagatgctga attttttatg gtcgttattt atcggcggta tattaggttt gatagctggc
    37741 gcaattttag gaaaggaagt cccgggaggc gtatctggaa atatcattat cggtttttta
    37801 ggtagttggc tcggcgaatt tttactagga ccactgggac cggaactcgg cggtttttat
    37861 ttgataccag cattattggg tgcaattttt tgccttgctc tttattcatt tgtggcaaga
    37921 cgtatcagag aacgtccaaa gaaagaataa ttttattcaa ataagttgac acttcagtgt
    37981 gttacttgga taattagaag caatttagga aagcgggtgt catggataag aatatcatct
    38041 atcattcaag cttacctaaa ggatatccat ataacaagaa atgagggaaa aacgtggcaa
    38101 caacgcacaa taccaaaaag atatcaatga ctggcctgct tgtagcgatg ggagtcgtct
    38161 acggagacat cgggacaagt ccgctttacg ttatgaaatc gattgttgaa gataatggcg
    38221 gattgtcagg aatcaatcct gattttgtta tcggatcagt ctcattgatt ttttggacat
    38281 tgactctact gacaacaatc aaatatgtcg tgattgcatt gaatgcagat aaccatggtg
    38341 aaggtggaat tttctcactc tacacacttg tccgaaaagg tgggaaatat ttgatcattc
    38401 ctgcaatgat cggaggagct gccttactag cagatggggt attgacccct gctgtgacgg
    38461 taacgactgc gattgaaggc ttgcgaggaa ttccgcagtt ttttgaccgg ttcggtaata
    38521 accagaacgt gatcgtcatt atcactttag ttattatttt tgttttattc atggtccaac
    38581 gctttggtac agatattgtc ggaaaagcat tcggaccaat catgttcgct tggtttactt
    38641 ttttaggcgt aatgggatta atgaacttca gtcaagactg gacagttatc cgtgcactga
    38701 atccttatta tgctatccag ctgcttttta gtgcagataa caaattaggc ttgttcattc
    38761 ttggaaatat tttcctggct acgacaggtg cagaggcgct ttattccgat cttggacatg
    38821 tcggcaaacg aaatattcgt ctgagctggc cttacgtcaa aatttgtttg gttatcaatt
    38881 attttgggca ggctgcgtgg ttgttgaatg tttatcaaaa ccctgaagca cagcaaatca
    38941 agaacctgaa tccattcttc caaatgatgc ctcaaagttg gatcgtgatc ggcgttgctt
    39001 ttgcaacagt cgctgcagtc attgcttcgc aagcattgat tacaggttcg tttacgctag
    39061 tttcagaagc aatcaagttg aaactgctgc ctcggttaaa gatcatgtat cctgggaaca
    39121 gtatcgggca aatgtatatc ccagcagtca atctgatttt atggatcgcc tgttctctag
    39181 tggttgtcac attccgaacg tctcatcata tggaagctgc ttacggtcta tcgatcactg
    39241 tgacgatgtt gatgacaaca gcactgcttt acttttatct tatccaaaat gggcattctc
    39301 gttggcttgc atatttagtt acctttttct tcggtgcgat agaagtcgtt ttctttatct
    39361 caagtgttgt caaattcttc cgaggcggtt ttgtagccgt tctgattggt ttgatcatct
    39421 tagtcgtgat gtttatttgg gaacaaggga acattattcg cgaatcgtca accgaggatg
    39481 tgtctttgag agattatatc ccgcagctta gagaattacg tgatgatact tctttaccaa
    39541 tctatcagac aaatgtggtc ttcttggttc ctgatatggc tgaagataaa gttggccgac
    39601 agttcatcta ttcgatttta gataaacggc ctaaaagagc acgagtctat tggtttgttc
    39661 atgtggaagt gacggatgag ccatatacga aagaatatca agtagatatg atggacactg
    39721 attttattgt ccaagtgaat ctgttcttag gtttcagggt ccaacaggaa atcaacgtat
    39781 atgttcgcca agtcatccat gatttgatga aacaaggccg tttgccaaaa cagccacaaa
    39841 gatactctct gacaccaggg cgagaagttg gcgatttcca atttgtcctg attgatgaag
    39901 tagtttccaa tgtgacaact ctaggaaaat ggcaacgtca aatcatgcag gcaaaactgg
    39961 caatcaaaaa aatcgctacc tcgccagaaa catggtttgg gctagagtac agcgaagtaa
    40021 aacatgaaac tgttccgctt atcattggaa atactcggaa aacatggttg aaagagcggt
    40081 tgtgaaagta gcgctttgac ctgaactaat gtcgaatgtc agctacttga acaccgttta
    40141 tcagaggtca tgaaaaagct gtactgcaac aagcagtaag aacgagcaga taaaaacagc
    40201 ttctcatgtt tttcatctgt ttgggcttaa tgccgtagat gcaagctttt gaataccgtt
    40261 tttatcagag gtcatgaaaa agctgtttag caacaagtaa cgcaatgatt ttgaagtgat
    40321 aaggagatgt ggctgaaaaa tgccgcatct ccttttttat tcctaaatat gaatacgcat
    40381 tattttcaat ctttttgtag aaaaagggta caatagaaaa agagaaaggc gggagaatat
    40441 ggttatcaat ttcattattt tatttttgat gattgcaatt acttcatttt ttgtagcaag
    40501 tgaatttgct ttagtgaaaa tcagacgatc aagattggaa caactagaaa aggaaaatgt
    40561 aaagaacgcc aagctagctc ttcatgttac acaccattta gataactatt tatcagctag
    40621 tcaattagga attacgctaa ccggtttgat catcgggtgg gtcggtgaag gatctgtcgc
    40681 ggctttacta gaaccagtga tcggtaaact accttttagt tcggctatta gcagtacaat
    40741 ctctgtggcg ctgggattta ttttagttac gtatatcgat gtggttattg gcgaactgct
    40801 tcctaagagc tatagtatcg tcaatacgga gaaggtcgtc ttgttcgtag ttaaaccact
    40861 tcattatttt tataaagtaa tgtttccttt tatttgggta ttgaatcatt cagctgcagg
    40921 gctagggaag ctattaggtg tgcgtctcgt atctgaagga gaagagacat tgtctcaaga
    40981 agagctgaca ttagtcgcat tgaattctta tgaaaaagga gagttgacca aagaagaata
    41041 ttattatttg gaaaacgtgt ttgaatttga tgataccttg gcaaaagata tacaagtaga
    41101 ccgtacgtcc atgcaagtat tcgaagcttc agaaaccgtc aaagaagcga taaaagcttc
    41161 gattgaacaa ggacatactc gctatccggt tatcttagaa tcaaaagaca atgttttagg
    41221 ttatgtcacc ttgccagatt tgatcaagca atcttataaa gatgaccagc tgacagtaga
    41281 acagttgatt gaagagccga tcgtaacaac tgaaacgatc cctataaaaa aattattgac
    41341 gatcatgcga aaaaagggta agcatattgc tatcttaaaa gatgaatacg gagggacaag
    41401 cggcttagtg acgattgaag atattttaga agaaatcgtc ggggaaatcc gagatgagac
    41461 agatttggac gaagcattga tagcggaaca gtcagatggc tcttatatca tctccggcaa
    41521 actaacatta gatgatttcc agcgttattt tcatgtagag attccagagt ttgaagaaac
    41581 gaattttact acacttgcgg gctttgcttc tagccgatat aaagaaataa aagcaggaac
    41641 aatcattgag attgcatcat tccgtttcac ggtattagaa taccagcatg cacatatcga
    41701 ttatttcaaa gtagagtcca cggaaagaaa aacagaataa aagaaaaaag gctgtgacat
    41761 tttttgtcac agcctttttg ttatctaaaa aacctgtttt cggtaaacga agaagatatt
    41821 ttcaataaaa gttttcattt ttagccgcaa cgatttcttt taatcaaggc tatcttgctt
    41881 ctgcatgttc taatgtgaaa tgttcctctt cacccatgtg tttcaattgt ttcatcgaac
    41941 gcatataaga gtaaagcaac acggcaacag aaccgatcca agctaaagga gaagctgctg
    42001 ctgctcctgg gtatccccac attcctgtaa gaacgatagc tgcaaaagaa cgcataagca
    42061 attccatcat accggcgatc gtagggatct tgctttgtcc aagcccttga agtgtgtagc
    42121 gtaaaatgaa caggactgct aagatccagt aaagagaccc attgatattg aagtaggttt
    42181 gtgctaattc aaatacacgt gtttctgatg ggttaacaaa caattgaaca aagaaatgac
    42241 cgaagaaaat gacgatagca cctgctaaga gactaaatcc gacactcatc agtaaagttt
    42301 gacgtacccc ttgcaagatt cggccatatt ctttcgcacc atagttttgt gcagtgaaag
    42361 ttgccattgc aataccaaat gacatcatcg gctgatttgc aaattggtcg atacgaccag
    42421 cagcggcttg agcagctacg acatctgtcc ctagactatt tagtgctgct tgtaacacga
    42481 tagcaccaac agcgatgatc gaagattgaa acgccattgg caaagcagca tttaggtgta
    42541 cagcaaactc ttctttttca aatttgaaat cttttttgcg taactgtaac aaaggcattt
    42601 ttttcttgat atacactaga cacagtacgc tggaagaaac ttgtgcgatt actgtagcaa
    42661 tagctgcacc ttccacaccc atgccaaaat agataataaa gatcaaatca agtacgacgt
    42721 tgatgatgac cgcaatgatc agaaagaata gtggtgtgcg gctgtcacca agtgcgcgaa
    42781 tgacattaga aagcaaattg aaactgactg atgcaaagat ccctaataaa ataattgaaa
    42841 taaatgtttg tgcttgatca agtatttcag gaggagtttg catcaattga agcatcggac
    42901 gaataaacag gagacttagg acagttaaaa tcacagtgac gacaagacta atgacaacac
    42961 tagcagcaaa acttttcttc agaccccgat aatcttttgc accgtaacgt tgtgcggtaa
    43021 taatggctaa accagctgtt aaaccttggg caaagccaat gatcaaaaat gtcaggcttc
    43081 cagttgcacc aaccgctgcc agcgcatttt tacctagcgt ctgacccacg atgatcatat
    43141 cgatcatatt gtagaactgc tgaaaaacat ttccgatcag taaaggaaca gtgaacatca
    43201 aaatcagttt agcggggctt ccttttgtta aatcgcgcat cttttaaact ccttatatct
    43261 ttaaaattta gttagtagta tatagcgttt atcctgagaa taccaattag aaatcaatga
    43321 taatttatga aactccataa aaagaagcca agaattgagt caaagaatca acaacttggc
    43381 ctggtctgta aaataaggga catccacgtt tatctatcac cttaatattg tctaatctca
    43441 cttggtaaaa ggatcgtttc tctcgtaccg tcatcatagg cgtatacttg agcaggaaat
    43501 tggttcgaat aggcaaacgg caaatcaata tccatttcca cattgcttcc tgcttgagaa
    43561 aaatgcatcg tcacttttcc acgattttcg atcaaatcaa aggttaatat attttctaat
    43621 ggaatgactc ccttaagatc taggtcaata ataaaccata tactgtcaat caattcatct
    43681 ggcaaacttg atacgacacc aactgaagcg taacggttgc ggctgctatc aaaactttca
    43741 aacatctgtt taacctcctt cgaatcaacg aatgttttat ttttataatt aatcctagga
    43801 taaactagaa atacttactt caacgagggt tcattctaat aaagaatcag gaaaaatgca
    43861 acaataattg aatacttttt ttcaaagtat aaatttttat aaaggtacga taaaagtatg
    43921 cgaggaaaag cttggtaaat taggattttt cgtttgtaaa gccatttaca aaagtggaag
    43981 ccccatatga aaataaacga ttagaaataa aaaaggcgtg ttaaaagctg ctgacccgcc
    44041 gcttctaaca ccacctgatt gtttcagtat tattcttctg ttgaaccgat cacttctggt
    44101 tcgccacctt cacctgtgct ttcttcttct tcactttgag cgactacagc agcgatttgc
    44161 tcttcactgt cggtcacgaa tttgtaatcg tcgttttttg gaagatcggc tactgtaata
    44221 gaatcaccta attccaagtt tgtcacgtca acttcaattc gttcaggcaa tttgtctggt
    44281 gtagcagata cgactactgt atacaagttt tgagctaaca ctccaccaga tttagcaccc
    44341 actgattcgc caacaagaac gacttctgtt tcgacttctg tttcttccga catgtttaca
    44401 gatagaaatt ctgcatgttc gaaatgattg gtaaaagtgt tcgtttggat cttgtgaacc
    44461 aatgtattga ctttttgtcc atcgatgtcc atcgtgatga ctgtgttcgc tccatgttca
    44521 cgcaacaatt tgcttagtgt caaaccatcg aatgagatag gggtactttc tactttatac
    44581 ccataaacaa cagctggaac tttcccttcg tgacgaagtt tgtttcttaa tgaacgggga
    44641 cgtacttctc ttttacttac ttctaatgat actgacataa ccaaaattcc tccttaaaat
    44701 gtgactgtct gctttggtaa cggacagatt ttatttaagt gaattctaaa ggctaatctc
    44761 gtgcacatca tcgaaggcag ggcgcttaaa aaagcgcaaa aggagagatg cgcgcatctc
    44821 cccttagaaa gtcaggattc aacttttctc caccaggtca ttacgctgtg tatgagaaat
    44881 ccaattcact ttactattta accgtaacgt gaactgttaa ttaagtcaag gaataactat
    44941 gatgatgtat ttgcaagcaa gaatattcaa ggtataatta attagtagta ataagaaagg
    45001 aaacagcatg cgtttagata aattaataga agaaaaatta gaaacgacac gcaaggaaat
    45061 gaagcggctg tttgctttga agcaagtgat gatagatggc acagtcgaat ttcgacaaaa
    45121 tcgaaatgta gatagtttac tgcatcaaat cgaagtagca ggtgaacact tagaaacaaa
    45181 tgagtcctat tatcttctca ataaacctga aaaagctgtt acagctgtat cagatccaga
    45241 aaagacgaca gtaatcgatc tgattgcaag tgccgatcgt ttccaaccat tatatccagt
    45301 gggaagacta gatagagata caacaggact attgctgatc acttcgaatg gacaattggg
    45361 atatgaaatg ctacttccag aaaaaaaggt ttcaaagact tatcaagtag ttgtgaacga
    45421 aagggtgaca ccggcagacg taacagcatt tgaaaatgga atcgtattcg ctgatgggta
    45481 tcaatgtaag ccggctgatt taaaaatact caaagcagac aagcaccaaa gtacggtttt
    45541 attgacgatc caagaaggaa aattccatca agtcaaaaag atgttcttag cccaaggaaa
    45601 gaaagtgacc gcattgaaac ggctaagcat gggaccattg gaattagatg aacagctagg
    45661 agtaggcgag tatcgatcgt tgacattagc agagatgaaa aagttaagta tttattttaa
    45721 ataggaggtc aaaaagatga aatataaaac tttattgttt gatgtagatg atacattgtt
    45781 agattttcaa ttaactgaag ccaatgcttt aagtgctttg tttgcagaac aaggaatcac
    45841 attgacacca gaaatcgaaa ccagctataa ggctttgaat catcatttat ggcaggagtt
    45901 tgaaaaaggc agactatcac gagaagaaat aataggcagt cgttttgggt tacttttcca
    45961 gcaatttggg aaaacagtag atagtgatca aatggaaaaa cgctaccgcc actacttgaa
    46021 ccaaggtcat gatcttgttc caggaagtct agaaatcctg aaaaaggtcc atccgcatgc
    46081 tgatctttat attgtgacga acggtgtgtc gcacacgcaa catcggcgat taacagatgc
    46141 aggaatgctt gattacttca aggatatctt tgtctcagaa gctgtcggag cgcaaaagcc
    46201 gatgaaagaa ttttttgatc atgtcttttc ccacattcct aattttgata aaaagcaaac
    46261 tgtgatcatt ggtgattctt tgacttctga tatcaaagga ggaaatatgg cagggatcga
    46321 tacgatttgg ttcaatagaa ataaattacc agaaatcgaa gaaatccagc ctacttatcg
    46381 aatcgattca ttagaggaac tttattcatt attggaaatt gaaaaggatt aattttcaga
    46441 aaaatatagc attttcgtgt tttttcgcta taatagatta gaatgaaaaa acggatacga
    46501 aagaggtttt agtcaatgac aaaaagcaag ccaattatca ttggtgtgac gggtggatcc
    46561 ggaagcggaa agacaagtgt cagccgtgcg attttcaaca acttcccgga ccattccatc
    46621 atgatgcttg aacaagattc ttattacaag gatcagagcc atttgagttt tgaagaacga
    46681 ttgaacacaa attacgatca cccatttgcc tttgataatg atctgctgat tcagcatgta
    46741 ggcgacttac tgaactataa agccattgaa aaacccgtgt atgactatgt agcgcataca
    46801 agaagtcagg caacgatcat tcaggaacca aaagaagtga tcattctaga aggaatcctg
    46861 attttagaag acgaacgtct gcgtgacttg atggatatca aagtatacgt agatacagat
    46921 gatgacattc gtatcattcg tcgtatcaaa cgggatatgg aagaacgggg acgcacattg
    46981 gactccgtga tcgaacaata tttgacagtt gttaaaccga tgtatcatca atttatcgaa
    47041 ccaacgaagc gctatgcgga tatcattgtt ccagaaggcg gagaaaacca cgttgctatt
    47101 gatttgatta caacaaaagt agctagtttc ttaaaccata aatagtgcgc agcgaaacag
    47161 ttgaacggac tgcgaccgtt tgaaaagcaa ggattctgct ttttgaacag tcaccagcca
    47221 actgtttttt tatttataac aagaggttgt gaaaacagca tcttgaccta agtattaaaa
    47281 agaagaaacg gaaaatagct gtttatattt taccgttttt tgagctaata ccgaaggtca
    47341 gctgattgag caccgtgaag tagaggttgt aaaaaagctg tctttgcatc aagcgataag
    47401 agcatccaaa taaaacagct cctcacgttt ttcgttgggt tgggcttatt atcgtagatg
    47461 caagcttttg aacaccgttt aacgagaggt tgtaacagaa gtggtcagat gcaataaata
    47521 agaagaaact tacgaaaatt atttctcgaa tttgtgtgag atttcgactt atttttttga
    47581 agaagctctt ttgtcgcacc cccta
//
LOCUS       DAJSVD010000010        46138 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSVD010000010 DAJSVD010000000
VERSION     DAJSVD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.010"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(65..376)
                     /gene="rhaM"
                     /locus_tag="ON498_000459"
     CDS             complement(65..376)
                     /gene="rhaM"
                     /locus_tag="ON498_000459"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR3938759.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(378..1223)
                     /gene="rhaD"
                     /locus_tag="ON498_000460"
     CDS             complement(378..1223)
                     /gene="rhaD"
                     /locus_tag="ON498_000460"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR3938760.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(1237..2493)
                     /gene="rhaA"
                     /locus_tag="ON498_000461"
     CDS             complement(1237..2493)
                     /gene="rhaA"
                     /locus_tag="ON498_000461"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR3938761.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(2490..3950)
                     /gene="rhaB"
                     /locus_tag="ON498_000462"
     CDS             complement(2490..3950)
                     /gene="rhaB"
                     /locus_tag="ON498_000462"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR3938762.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            4103..4972
                     /locus_tag="ON498_000463"
     CDS             4103..4972
                     /locus_tag="ON498_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR3938763.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(5098..5997)
                     /locus_tag="ON498_000464"
     CDS             complement(5098..5997)
                     /locus_tag="ON498_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3938764.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(6162..6986)
                     /locus_tag="ON498_000465"
     CDS             complement(6162..6986)
                     /locus_tag="ON498_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3938765.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(6997..8292)
                     /locus_tag="ON498_000466"
     CDS             complement(6997..8292)
                     /locus_tag="ON498_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR3938766.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWAPGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(8308..8787)
                     /locus_tag="ON498_000467"
     CDS             complement(8308..8787)
                     /locus_tag="ON498_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3938767.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(8793..9821)
                     /locus_tag="ON498_000468"
     CDS             complement(8793..9821)
                     /locus_tag="ON498_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3938768.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(9838..10944)
                     /locus_tag="ON498_000469"
     CDS             complement(9838..10944)
                     /locus_tag="ON498_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3938769.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(11173..13770)
                     /gene="adhE"
                     /locus_tag="ON498_000470"
                     /gene_synonym="adhC"
     CDS             complement(11173..13770)
                     /gene="adhE"
                     /locus_tag="ON498_000470"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3938770.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(14075..14455)
                     /gene="yajC"
                     /locus_tag="ON498_000471"
     CDS             complement(14075..14455)
                     /gene="yajC"
                     /locus_tag="ON498_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3938771.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(14552..15697)
                     /gene="tgt"
                     /locus_tag="ON498_000472"
     CDS             complement(14552..15697)
                     /gene="tgt"
                     /locus_tag="ON498_000472"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3938772.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            15915..16613
                     /locus_tag="ON498_000473"
     CDS             15915..16613
                     /locus_tag="ON498_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3938773.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(16781..19420)
                     /locus_tag="ON498_000474"
     CDS             complement(16781..19420)
                     /locus_tag="ON498_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3938774.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(20039..21070)
                     /gene="queA"
                     /locus_tag="ON498_000475"
     CDS             complement(20039..21070)
                     /gene="queA"
                     /locus_tag="ON498_000475"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3938775.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            21263..22438
                     /locus_tag="ON498_000476"
     CDS             21263..22438
                     /locus_tag="ON498_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3938776.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            22442..23083
                     /locus_tag="ON498_000477"
     CDS             22442..23083
                     /locus_tag="ON498_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938777.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            23080..23997
                     /locus_tag="ON498_000478"
     CDS             23080..23997
                     /locus_tag="ON498_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3938778.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            23994..24665
                     /locus_tag="ON498_000479"
     CDS             23994..24665
                     /locus_tag="ON498_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938779.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(25206..25694)
                     /locus_tag="ON498_000480"
     CDS             complement(25206..25694)
                     /locus_tag="ON498_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR3938780.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(25895..26161)
                     /locus_tag="ON498_000481"
     CDS             complement(25895..26161)
                     /locus_tag="ON498_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3938781.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(26161..26424)
                     /locus_tag="ON498_000482"
     CDS             complement(26161..26424)
                     /locus_tag="ON498_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3938782.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            26900..27085
                     /locus_tag="ON498_000483"
     CDS             26900..27085
                     /locus_tag="ON498_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3938783.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(27227..29050)
                     /locus_tag="ON498_000484"
     CDS             complement(27227..29050)
                     /locus_tag="ON498_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3938784.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(29648..32353)
                     /locus_tag="ON498_000485"
     CDS             complement(29648..32353)
                     /locus_tag="ON498_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR3938785.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(32659..33606)
                     /locus_tag="ON498_000486"
     CDS             complement(32659..33606)
                     /locus_tag="ON498_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR3938786.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            33796..34065
                     /gene="rpsN"
                     /locus_tag="ON498_000487"
     CDS             33796..34065
                     /gene="rpsN"
                     /locus_tag="ON498_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3938787.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(34241..34603)
                     /locus_tag="ON498_000488"
     CDS             complement(34241..34603)
                     /locus_tag="ON498_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3938788.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(34614..35735)
                     /locus_tag="ON498_000489"
     CDS             complement(34614..35735)
                     /locus_tag="ON498_000489"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3938789.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(35751..36101)
                     /locus_tag="ON498_000490"
     CDS             complement(35751..36101)
                     /locus_tag="ON498_000490"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3938790.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(36231..37742)
                     /locus_tag="ON498_000491"
     CDS             complement(36231..37742)
                     /locus_tag="ON498_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3938791.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(38064..39416)
                     /locus_tag="ON498_000492"
     CDS             complement(38064..39416)
                     /locus_tag="ON498_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3938792.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(39569..40645)
                     /locus_tag="ON498_000493"
     CDS             complement(39569..40645)
                     /locus_tag="ON498_000493"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3938793.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            40841..42586
                     /locus_tag="ON498_000494"
     CDS             40841..42586
                     /locus_tag="ON498_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR3938794.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(42866..43303)
                     /locus_tag="ON498_000495"
     CDS             complement(42866..43303)
                     /locus_tag="ON498_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3938795.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(43287..44660)
                     /locus_tag="ON498_000496"
     CDS             complement(43287..44660)
                     /locus_tag="ON498_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938796.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(44982..45470)
                     /locus_tag="ON498_000497"
     CDS             complement(44982..45470)
                     /locus_tag="ON498_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938797.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(45492..46094)
                     /locus_tag="ON498_000498"
     CDS             complement(45492..46094)
                     /locus_tag="ON498_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3938798.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        13145 a        10007 c         8334 g        14652 t
ORIGIN      
        1 ttgtattatt tttcacaata aaaacgaaag aaccccaagc aaccggttgc ccagttgctt
       61 gttgctacag ttcaaagact ggcatcaatt cccaactgac tggagaggca tctggatttg
      121 tctccatgat atcttccatg tacttccacc atttttgatt gatagatgta tttgccattt
      181 tttcccataa cttttcatct tcgatcacta agtaaccaaa taacaaactt gtactcggat
      241 caagatagat cgaatagtcg atgaccccgt gttcacgaag tgctgtacgc atttctggcc
      301 aaagttcatc atgtcttttt ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa
      361 aagctttttt caccatgtca gacacctgct ttcgtatgaa cttcctccaa ataacctgct
      421 tttggagtga caccaaacgc atctgctaaa tgccacaggt tttcgtccgt gattgtttgc
      481 aagattggtc cttgcgcttt gacgtacgta tagacttctg ctgccttttc tgctgtctcg
      541 atcaaaccaa agacttcatc catgtctttt cctgacccgt agattccatg ttgtggccat
      601 aaaaccaatc gagtttcttt cattttttca gctgttgctt ccccaatctc atttgttccc
      661 ggaaccatcc acggaatgat ggaaattcct tctggaaaaa caactaagca ttccgtacac
      721 atttgccata gcgtacgact aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct
      781 aatagatgac tagcatggca atgcataacg atccgattat ctgggtctac ttctaaacgg
      841 gcgatatgac tcatgaaatg acttggcaac tcgcttgtag gaaggcattc gttttccagc
      901 ccccacaaaa gttccagtgt tttgccatct tcagcaactc gaacgattcc aagattctcg
      961 gcaggttcgc tcgctacatt tttaaaatat cttcctgaac cagttacaat aaaatagcat
     1021 cctgctaaac ggctggcatc aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc
     1081 aagaacggtg tgatctcttc ttcttttagt aaataagaaa tattcccgcc atttctttca
     1141 tcccatccca agcgataaag attcgacgtc gtttcgatca tttctttcac ataaggcgct
     1201 tgtaaaatat ctattttttt caatatagcc acactcctat tttcttaatt ccaacacgtc
     1261 tttttgatat tcctggactt cttccagcca atccaaacca actggaactt gatttttgta
     1321 acagaaataa ttccatacat cggcatacgg gaaatctttc aattcttccg tataagccat
     1381 tcttgaagta aagtcaaaag ccagctccat ttctttcaat cgctcaactg gttccagcat
     1441 agctttcagc agtgcttttt gtgtatttct cgtaccgatg acccaagcag cgatacggtt
     1501 gatcgttgca tcaaagaaat ccagaccgat atttgttttt tccaacaatt cgttacggac
     1561 taattcttta gcgatttcct gcaattcatc atccatcaaa accacatggt ctgaatccca
     1621 acgcactgga cgggaaacat gcaacatgat tcctttgctg aataaagcta atgaagataa
     1681 tttattggaa atcacttctg ttggatgaaa atgtccagca tctagacaaa caagtttgtc
     1741 acgagtcaac ccgtaaccca tataaaattc atgggaaccg actgtataag cttcagcgcc
     1801 taaaccaaat agtttgcttt ctactgcatc ttgtacatac cgttcatcta actcttctgt
     1861 aaaaatttca tcgagtgatt ccattaaacg ttcccgtggg gtaaaacgat caatcggatt
     1921 atctttcatc ccatcaggta cccaaaagtt attgatactt gtttgcccag tttctttccc
     1981 aaagtactcc gcgattttgc ggcttctttt cccgtgttcg atccagtatt cacgaacttc
     2041 tttatctgga tgagccaatg taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg
     2101 attgaagtca agaccgattt cttctttttt tgcccactct acccattttt caaaatgttt
     2161 cggctcaatt tcatttagat cgacagattc ttctgtatcc aaatagatag catgaaggtt
     2221 caatttgtgt ttgcccggta tcaacgaata agccttctcc agatcttgtc taagctcttc
     2281 aggtgttcgt gcggctcctg gaaaattccc tgtcgccatg attccaccgg tcaattcgcc
     2341 atccggtgtc aagaaacctt tgacatcatc tccttgccag caatgcatag aaattttgat
     2401 gtcctgtatc ttttctagta cagcatctgt atctacaccg atagttgcat atttttcttt
     2461 tgcttgttcg taatttcttt caatcgtgtt catacgatta ctcctttgat tttattcacg
     2521 taccattcca tcgtgttttg cttattttca tctgggtaaa aagtttcaca tggaaacgac
     2581 tgtttgatta catttcttgc ttgttctata ttcgaaaact gacctgtact gagcatttgc
     2641 atggcaacat tcccgattgc tgtggcttca ctaggtcctg cttctacaat gattccggca
     2701 taatctgctg tcaattgatt cagtagacga ttattcgatc cgccccctac gatgtgcagc
     2761 ttttccaatt tcgttccagt taagtgttct actttgatca gctcttgtgc gtaacaaaga
     2821 gcaagattat catacacaca acgagcgata gctcccgatg agacagggac tggctgctct
     2881 gtttcaaaac agtattgctg aatctcttct atcatatttg ggggattcaa aaatcgaggg
     2941 tcattgattt ccatcacttg ctgcagcggt ttttcttttg cagcaagctc tgccaattcc
     3001 gcataactgt actcgtactc ttgcattcgg gcaacttcct ggatcaacca catccccatg
     3061 atgtttttca aaaaacgcac cgtgttatgg gcaccccatt cattcgtata attttcagaa
     3121 aaagcattgc gagaaacatt ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca
     3181 ctgctgatat aaccccaacg ttctccttga cctggcgttc ctaatacagc tgacgccgta
     3241 tcatgacttg ctactgtgat aaaggtcaca tctggcaaat catattttga aaattgttct
     3301 ttttgcagat ctcctagtat cgttcccggc tctgtcaatg gtggaaataa ttccttcgag
     3361 acaccgattt tttcaagaag ttctatttcc cattcttttg tccctgcatt cagcagctgc
     3421 atagtagatg cattagtttt ctccgtcacc atgttaccag taaagacata ccctaaataa
     3481 tcaggtatca gtaacaattt atgtgctttt gccaaatttt ctttttcttc agcaaacaat
     3541 tggaacaagg tattgaaagg ctggatctga atccctgtct gctcatacaa cttatctaga
     3601 gaatagattt cagaaaactg ttctactgct ccattggtac gcttgtcccg ataagcaatc
     3661 ggatcattta tccgatcccc ttgttcatca atcagacagt aatcgactgc ccatgtatcg
     3721 atccccgcaa aaaggcgatc aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc
     3781 tctttgataa gataatcgat cttccagcga tcgtgaccgt ctttgttccg aaagccgttt
     3841 ttgaaccggt gtacttcttc caagatcaaa gaaccttttg catccctcgt acttttcatt
     3901 agtcgtccgc ttgatgcacc gatatcgatg gcaacaaatg ttggattcat ttctccacct
     3961 cttttacttt attgttgatt agcactttat taataaagcg ttatcattta ttgacaatgc
     4021 tatcataata cagaatctta tattttgctt cacactattt tggtatataa ttgcattatc
     4081 ttgacaagtt gggagtgttt acatggatta ctgggaaaaa gccaaccact catggtcgga
     4141 agactcaaaa agagtgatct tgacgcctac acaaagaagc aggaatttat ttttttatat
     4201 ccaagaaatc ggttacttca aagcaccaat cccttactat actgaacgag cacatttacc
     4261 ttcttacttg ataaagttta ctttgtctgg caaaggttcg cttacatata atgataatca
     4321 gcaacagcta aaagctggag acttattttt tattgactgc caagaatatc agcgttatca
     4381 aacgatcagc gaagaaccat gggaaatgga ttggatccat ttgaatggtc agaacgtcca
     4441 atcattttat caggagttca caaaagacgg ccataacacc ttccattcaa caggagatcc
     4501 atcagaaaac aagatccacc atttgatcac agaattgatc cacatgcaac aaggcacgaa
     4561 tgcccgtacc gatttccagt cctctgtagc tatccacgag ttgctgaatg aactgatttt
     4621 gcaaaagtat cagctagatt tcgaagaaga ggatattccc gcctacgttt acaaactgaa
     4681 gcaatactta gacgaacatt tcaaagaaac gattacactt gatcaattag aacaacaatt
     4741 cttattgaat aaatatcagc tgagcaaaga tttttccaaa tttatcggtt ctccaccaat
     4801 cgattatttg atcagcaaaa aactttctta cgctaaagac cttcttcggt atacagaatt
     4861 tacggttcga atgatttccg aagaaatagg gattgagaat tttgcttact ttagccgcct
     4921 gttcaaagca aaaacaggac tatcgccgag agactttcgt aaattcagct gattcatata
     4981 atatgtacgt cattttttca aaatattttg aaatatatag ataaaaataa aataaccgca
     5041 gtttctgctt ctatgtaaac aacagcagct actgcggtta ttttttagtg atcattctca
     5101 ttgttcatct tcaaaatgtt cataatgaac caactgtttc tgtgtatcac gtttcaaaaa
     5161 ttctttaggc gttatgccga actctctctt gaatgctttc agaaaatgcg gataagaaga
     5221 aaaaccactt ttactatata cagcactaga gctgcggtat tctcgcaaca agtgcttggc
     5281 atagatcagc tttttcttca ttacatactg atgaaacgta aatccagtgt actttttaaa
     5341 ttctcgtgtg atatggtatt tgctgacaaa aaactgcttt tccatttcct ctaaagaaag
     5401 cggatgagat aaattttttg agatatattg taacatttgt tccacacgct cattaggaac
     5461 tacagcattc tgttcttgcg cactctcttc tttttgtaca acttgattga gaaaaatcaa
     5521 gtattctacc agagcttgtt catattttat gtcttcaccg taaatttttt tatcaggagg
     5581 atggtcgata aatgataatt tttgcttcaa tacttcctgt tctatttgca atacacgact
     5641 cctcttctct cccattggtt gaaaacaagc cgatagatta gaccatggcg tggaacgact
     5701 ttctagaaaa gaaggggtga caaagatata gacacgctcg aatacatctg aagattggcg
     5761 gacgatacga tgcaaatcat tggaatggat gagcagcaca cttcctgctt tgatcgtaaa
     5821 ttgtcgtccg ttcaaataaa aagtcgcttc tccctttaat gtagcatgga tctcatagaa
     5881 atcatgatga tgatagatcg ttttattttg gctgacgaca tctttgacat gatagatttc
     5941 atactgatca gaaaccatcg tattgacttg ttcaccgctg attttctttt ggagcatgtg
     6001 ccgcacctcc ttttgttcac ttatgcacaa ttttattata ccacgttttt tgtaatcaaa
     6061 aagagctgct tttatatact cttataaaaa agaagagacg tggcatcttc tagccccgcc
     6121 ccctccattc taaatattag ttaaccaacg tcttctttac attattcaag aagacttctt
     6181 ccataacgaa tcccatcatc ttttgtttct tcaagaacca catacaggta cggacgattc
     6241 ttcatgatga tttctttgat ttttgtgtac tcgatcacac cgtctcccaa atttaccggc
     6301 acgatttttt cctgttcgac acgaaaatct ttcaaatgaa ccgcacatat cttttcacca
     6361 aaacgttcaa aagcttcttc taccaattgg acttgttctt gataatcact acttgtgatc
     6421 aaattggtcg gatctaaaat gattcctaga tagtccgaat ctactgcttg gatcagctgc
     6481 tctacccttg ctaaagaata caccggatga ttcaaacctg gttctatccc caccatcatt
     6541 tggtattttt ctccagcttt taccaagcga cggataacgg acacagcttc agcaaatgct
     6601 tcatctgtga aattttcttc cgtatattgg atttccggca aaacacatcc agtttccgaa
     6661 gcaaccatcg acgcaccaaa ggaagaagca tagcgaagat actgctcgaa ctttgtcagc
     6721 aactcttgtc gtatcgttaa atctggatga atcatattga tatagcaact taaaattcct
     6781 acggtcactt cttgcttttc taaaactcgt ttgacataat ttcccatccc agaattgatc
     6841 tcatttgcgc cagaaggtat ttcaggaaac gaaagaccta aggcgagctg cagtgtatga
     6901 atcccttgtt cttttgtttt ttctgcaaga tcttctatcg tttggacttc ggaaagatca
     6961 tgtcctctaa ttgtaatgtt caacataact attctcctaa ctcagcatat tgggtagcca
     7021 catgacgatt tgcgggaagt aagtaatcaa aaacaagacg actaagataa ccccataaaa
     7081 aggaagaagc ggtttcagca ttgcctctgc ttttacgcct cctacacttg caccgacata
     7141 tagtcctgta cctactggcg gcgtgatgga gcctacacac agattcatga ttgaaaacaa
     7201 cccgtaatgt acaggatcaa cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa
     7261 aatcaagata gcaggcgcca catccataaa catacccacc agtaataaca caagattgac
     7321 taataacaga ataataaatt tattatccgt tacgcccata atcaacgaac taattgcctg
     7381 aggaataccg gtaaacgcca tggcgaaaga catcatggaa gaagtagcca ataagaacat
     7441 gatcgtccct gacattgcta ctgattgaaa caacatatta ggtatatctt ttagttttac
     7501 cgttttatag taaaacatag caatcaacag ggagtaaatg acagctatcg ctgaagcttc
     7561 tactgctgta aatatccccg tcaagatacc accgataatg atgacaatca atagaaggct
     7621 aggaatcgcc tcttttatta tttttccagc atgagccatc tgtccttttt cgacaacagg
     7681 gtatttattc tttttagcaa cgatgaatgc tactagcatg attcctggcg cccacaaagc
     7741 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
     7801 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
     7861 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
     7921 gccaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
     7981 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
     8041 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
     8101 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
     8161 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
     8221 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
     8281 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
     8341 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
     8401 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
     8461 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
     8521 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
     8581 tacggctaaa tgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
     8641 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
     8701 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     8761 taataccttg tttaaaatca ttttcatcac agctacgctc cttgctcttg gatcttttga
     8821 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     8881 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     8941 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     9001 gcttcttcaa tggctttttc ctgctcatca gttagctgtt ctttgatggc atcattcatg
     9061 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc cacaccacca
     9121 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     9181 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     9241 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     9301 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     9361 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     9421 atcaccttaa agaaatgttc ttcatcattg aatagataag gaactgaaaa aatcgaataa
     9481 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     9541 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     9601 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     9661 actggctggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacatctgca
     9721 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     9781 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     9841 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     9901 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     9961 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
    10021 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
    10081 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
    10141 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
    10201 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
    10261 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
    10321 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
    10381 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
    10441 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
    10501 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
    10561 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
    10621 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
    10681 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
    10741 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
    10801 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
    10861 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
    10921 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
    10981 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
    11041 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
    11101 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
    11161 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
    11221 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
    11281 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
    11341 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
    11401 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
    11461 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
    11521 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
    11581 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
    11641 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
    11701 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
    11761 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
    11821 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
    11881 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
    11941 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
    12001 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
    12061 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
    12121 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
    12181 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
    12241 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
    12301 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
    12361 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
    12421 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
    12481 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
    12541 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
    12601 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
    12661 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
    12721 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
    12781 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
    12841 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
    12901 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
    12961 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
    13021 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
    13081 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
    13141 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
    13201 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
    13261 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
    13321 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
    13381 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
    13441 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
    13501 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
    13561 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
    13621 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
    13681 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
    13741 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
    13801 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
    13861 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
    13921 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
    13981 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
    14041 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
    14101 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
    14161 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
    14221 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
    14281 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
    14341 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
    14401 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
    14461 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
    14521 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
    14581 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
    14641 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
    14701 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
    14761 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
    14821 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
    14881 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
    14941 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
    15001 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
    15061 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
    15121 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
    15181 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
    15241 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
    15301 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
    15361 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
    15421 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
    15481 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
    15541 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
    15601 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
    15661 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
    15721 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
    15781 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
    15841 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
    15901 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
    15961 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
    16021 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
    16081 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
    16141 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
    16201 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
    16261 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
    16321 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
    16381 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
    16441 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
    16501 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
    16561 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
    16621 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
    16681 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
    16741 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
    16801 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
    16861 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
    16921 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
    16981 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
    17041 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
    17101 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
    17161 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
    17221 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
    17281 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
    17341 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
    17401 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
    17461 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
    17521 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
    17581 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
    17641 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
    17701 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    17761 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    17821 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    17881 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    17941 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    18001 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    18061 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    18121 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    18181 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    18241 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    18301 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    18361 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    18421 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    18481 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    18541 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    18601 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    18661 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    18721 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    18781 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    18841 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    18901 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    18961 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    19021 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    19081 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    19141 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    19201 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    19261 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    19321 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    19381 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    19441 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    19501 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    19561 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    19621 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    19681 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    19741 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    19801 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    19861 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    19921 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    19981 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    20041 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    20101 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    20161 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    20221 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    20281 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    20341 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    20401 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    20461 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    20521 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    20581 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    20641 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    20701 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    20761 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    20821 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    20881 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    20941 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    21001 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    21061 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    21121 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    21181 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    21241 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    21301 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    21361 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    21421 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    21481 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    21541 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    21601 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    21661 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    21721 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    21781 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    21841 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    21901 tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga
    21961 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    22021 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    22081 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa
    22141 aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga
    22201 cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca
    22261 acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt
    22321 aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga
    22381 tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc
    22441 tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct
    22501 ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt
    22561 actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac
    22621 aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc
    22681 tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg
    22741 gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt
    22801 ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg
    22861 tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg
    22921 tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg
    22981 tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca
    23041 ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg
    23101 accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc
    23161 aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta
    23221 gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac
    23281 aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca
    23341 gcggctcgct acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat
    23401 ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg
    23461 ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa
    23521 aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt
    23581 gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat
    23641 ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa
    23701 gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat
    23761 ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta
    23821 gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat
    23881 accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa
    23941 ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc
    24001 aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct
    24061 atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag
    24121 cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg
    24181 tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa
    24241 tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct
    24301 taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg
    24361 tcggcaaagg catggggatg actgctagac aacgactatt catggtagag ttaccactct
    24421 ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg
    24481 cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg
    24541 caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca
    24601 tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac
    24661 gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt
    24721 ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag
    24781 agttgctttt cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt
    24841 tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca
    24901 ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt
    24961 tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc
    25021 atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc
    25081 ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa
    25141 catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac
    25201 gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga
    25261 agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa
    25321 aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc
    25381 acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc
    25441 cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac
    25501 catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt
    25561 ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt
    25621 tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg
    25681 attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct
    25741 aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca
    25801 ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt
    25861 tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat
    25921 aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca
    25981 gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca
    26041 tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt
    26101 gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca
    26161 tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg
    26221 gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct
    26281 ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat
    26341 ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct
    26401 aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta
    26461 cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct
    26521 tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta
    26581 ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag
    26641 caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc
    26701 gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta
    26761 acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct
    26821 ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt
    26881 tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga
    26941 ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc
    27001 tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat
    27061 caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc
    27121 atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag
    27181 cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat
    27241 ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca
    27301 aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca
    27361 acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga
    27421 taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga
    27481 aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa
    27541 catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta
    27601 cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg
    27661 catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa
    27721 aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga
    27781 caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt
    27841 tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag
    27901 caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga
    27961 aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta
    28021 gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg
    28081 caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag
    28141 caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca
    28201 aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca
    28261 cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc
    28321 ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag
    28381 aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg
    28441 gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga
    28501 tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt
    28561 ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga
    28621 cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag
    28681 atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat
    28741 tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga
    28801 tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg
    28861 gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg
    28921 taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg
    28981 ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta
    29041 agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa
    29101 tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag
    29161 aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca
    29221 aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag gatagaactt
    29281 gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag
    29341 gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg
    29401 ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa
    29461 gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc
    29521 ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat
    29581 gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc
    29641 tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt
    29701 cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca
    29761 tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca
    29821 agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga
    29881 cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga
    29941 tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta
    30001 aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta
    30061 agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca
    30121 gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat
    30181 agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag
    30241 tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta
    30301 gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga
    30361 tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga
    30421 taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg
    30481 tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta
    30541 gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg
    30601 gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat
    30661 ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt
    30721 gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg
    30781 aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga
    30841 tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa
    30901 tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct
    30961 tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta
    31021 gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta tttccagcac
    31081 ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata
    31141 ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct
    31201 taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt
    31261 gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca
    31321 gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt
    31381 tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat
    31441 cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt
    31501 gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt
    31561 gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta
    31621 ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta
    31681 gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat
    31741 ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt
    31801 aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg
    31861 tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga
    31921 ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca
    31981 gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga
    32041 ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat
    32101 gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct
    32161 tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg
    32221 ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg
    32281 ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt
    32341 tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat
    32401 ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca
    32461 attgtctcat tgtaatctct tacatattga gaaataatta aggattacag cctttacttt
    32521 atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac
    32581 gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt
    32641 tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg
    32701 tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga
    32761 aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc
    32821 atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa
    32881 atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat
    32941 gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc
    33001 aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt
    33061 taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag
    33121 aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt
    33181 cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat
    33241 tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa
    33301 cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg
    33361 aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac
    33421 ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata
    33481 aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg
    33541 atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct
    33601 tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa
    33661 aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta
    33721 acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat
    33781 tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa
    33841 acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc
    33901 ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat
    33961 ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta
    34021 gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt
    34081 aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct
    34141 tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc
    34201 cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca
    34261 gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat
    34321 ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca
    34381 ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa
    34441 gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt
    34501 tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga
    34561 ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct
    34621 tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct
    34681 aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc
    34741 acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca
    34801 cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt
    34861 ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata
    34921 tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca
    34981 gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga
    35041 ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc atcatgccat
    35101 aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc
    35161 acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta
    35221 gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct
    35281 gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt
    35341 agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc
    35401 aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg
    35461 tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg
    35521 actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca
    35581 gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt
    35641 ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc
    35701 actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc
    35761 aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct
    35821 tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct
    35881 tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg
    35941 taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt
    36001 tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc
    36061 ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat
    36121 aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa
    36181 ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta
    36241 tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg
    36301 ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca
    36361 cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc
    36421 attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct
    36481 gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct
    36541 gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact
    36601 gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc
    36661 cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa
    36721 gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt
    36781 tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca
    36841 cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat
    36901 acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt
    36961 gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt
    37021 aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt
    37081 gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca
    37141 tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct
    37201 gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc
    37261 aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca
    37321 agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata
    37381 tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct
    37441 tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct
    37501 aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt
    37561 tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata
    37621 acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca
    37681 aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc
    37741 aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat
    37801 ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc
    37861 ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa
    37921 acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg
    37981 gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc
    38041 gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat
    38101 aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg
    38161 atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat
    38221 ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg
    38281 aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct
    38341 aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa
    38401 gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct
    38461 gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc
    38521 ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa
    38581 gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca
    38641 aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata
    38701 ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct
    38761 gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta
    38821 gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct
    38881 gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc
    38941 atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca
    39001 atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac
    39061 gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg
    39121 cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca
    39181 ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat
    39241 gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga
    39301 acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc
    39361 agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag
    39421 atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact
    39481 ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca
    39541 atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct
    39601 gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc
    39661 ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta
    39721 attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc
    39781 ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt
    39841 ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat
    39901 catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc
    39961 ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat
    40021 catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa
    40081 tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt
    40141 cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat
    40201 aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg
    40261 cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc
    40321 cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt
    40381 cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac
    40441 ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac
    40501 ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat
    40561 aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc
    40621 cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga
    40681 tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg
    40741 agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt
    40801 tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg
    40861 cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt
    40921 acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat
    40981 caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac
    41041 attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat
    41101 cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt
    41161 tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt
    41221 cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc aactatttga
    41281 acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt
    41341 actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa
    41401 tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta
    41461 tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa
    41521 tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca
    41581 gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga
    41641 tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga
    41701 agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt
    41761 tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat
    41821 cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt
    41881 tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca
    41941 gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca
    42001 gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac
    42061 gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt
    42121 tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc
    42181 ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca
    42241 aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca
    42301 ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca
    42361 agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt
    42421 agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga
    42481 ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt
    42541 ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac
    42601 agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt
    42661 gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt
    42721 gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt
    42781 atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc
    42841 tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag
    42901 acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta
    42961 actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt
    43021 ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat
    43081 cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat
    43141 cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat
    43201 ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat
    43261 cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc
    43321 cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc
    43381 aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat
    43441 agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac
    43501 tggtggatca cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt
    43561 ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc
    43621 cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa
    43681 gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc
    43741 catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc
    43801 gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa
    43861 ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc
    43921 ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata
    43981 gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag
    44041 ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct
    44101 ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa
    44161 agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga
    44221 tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa
    44281 ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc
    44341 agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca
    44401 agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga
    44461 ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta
    44521 aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga
    44581 ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat
    44641 cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct
    44701 tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa
    44761 aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag
    44821 acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat
    44881 agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc
    44941 tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc
    45001 attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc
    45061 tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa
    45121 aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa
    45181 gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca
    45241 tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag
    45301 gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg
    45361 taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg
    45421 actgccatca gctcatccag ttcattttct gttactttta ttttttccat acacccacgt
    45481 ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta
    45541 tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat
    45601 aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag
    45661 gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc
    45721 tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc
    45781 aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca
    45841 ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc
    45901 acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata
    45961 aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac
    46021 acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat
    46081 ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact gcctcctt
//
LOCUS       DAJSVD010000011        45319 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSVD010000011 DAJSVD010000000
VERSION     DAJSVD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.011"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            150..1340
                     /locus_tag="ON498_000499"
     CDS             150..1340
                     /locus_tag="ON498_000499"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3938799.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1442..2395
                     /locus_tag="ON498_000500"
     CDS             1442..2395
                     /locus_tag="ON498_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3938800.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2464..3813
                     /locus_tag="ON498_000501"
     CDS             2464..3813
                     /locus_tag="ON498_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3938801.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(3996..4319)
                     /locus_tag="ON498_000502"
     CDS             complement(3996..4319)
                     /locus_tag="ON498_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938802.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4448..5611
                     /locus_tag="ON498_000503"
     CDS             4448..5611
                     /locus_tag="ON498_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3938803.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            5681..7030
                     /locus_tag="ON498_000504"
     CDS             5681..7030
                     /locus_tag="ON498_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3938804.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7075..7506)
                     /locus_tag="ON498_000505"
     CDS             complement(7075..7506)
                     /locus_tag="ON498_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938805.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            7894..10536
                     /gene="alaS"
                     /locus_tag="ON498_000506"
     CDS             7894..10536
                     /gene="alaS"
                     /locus_tag="ON498_000506"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3938806.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            10736..11332
                     /locus_tag="ON498_000507"
     CDS             10736..11332
                     /locus_tag="ON498_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3938807.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11454..12602
                     /locus_tag="ON498_000508"
     CDS             11454..12602
                     /locus_tag="ON498_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3938808.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12640..13053)
                     /locus_tag="ON498_000509"
     CDS             complement(12640..13053)
                     /locus_tag="ON498_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR3938809.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13132..13563
                     /locus_tag="ON498_000510"
     CDS             13132..13563
                     /locus_tag="ON498_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR3938810.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(13662..13895)
                     /locus_tag="ON498_000511"
     CDS             complement(13662..13895)
                     /locus_tag="ON498_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR3938811.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14078..15748
                     /locus_tag="ON498_000512"
     CDS             14078..15748
                     /locus_tag="ON498_000512"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR3938812.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            15895..16281
                     /locus_tag="ON498_000513"
     CDS             15895..16281
                     /locus_tag="ON498_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938813.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16268..16966
                     /locus_tag="ON498_000514"
     CDS             16268..16966
                     /locus_tag="ON498_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3938814.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            16971..17435
                     /locus_tag="ON498_000515"
     CDS             16971..17435
                     /locus_tag="ON498_000515"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3938815.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17432..18343
                     /locus_tag="ON498_000516"
     CDS             17432..18343
                     /locus_tag="ON498_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3938816.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18578..19117
                     /gene="pyrR"
                     /locus_tag="ON498_000517"
     CDS             18578..19117
                     /gene="pyrR"
                     /locus_tag="ON498_000517"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR3938817.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19131..20414
                     /locus_tag="ON498_000518"
     CDS             19131..20414
                     /locus_tag="ON498_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3938818.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20427..21353
                     /locus_tag="ON498_000519"
     CDS             20427..21353
                     /locus_tag="ON498_000519"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR3938819.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21372..22652
                     /locus_tag="ON498_000520"
     CDS             21372..22652
                     /locus_tag="ON498_000520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3938820.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            22657..23733
                     /gene="carA"
                     /locus_tag="ON498_000521"
     CDS             22657..23733
                     /gene="carA"
                     /locus_tag="ON498_000521"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3938821.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            23738..26923
                     /gene="carB"
                     /locus_tag="ON498_000522"
     CDS             23738..26923
                     /gene="carB"
                     /locus_tag="ON498_000522"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3938822.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            26977..27753
                     /locus_tag="ON498_000523"
     CDS             26977..27753
                     /locus_tag="ON498_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR3938823.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            27750..28682
                     /locus_tag="ON498_000524"
     CDS             27750..28682
                     /locus_tag="ON498_000524"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR3938824.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            28679..29395
                     /gene="pyrF"
                     /locus_tag="ON498_000525"
     CDS             28679..29395
                     /gene="pyrF"
                     /locus_tag="ON498_000525"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR3938825.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29402..30031
                     /locus_tag="ON498_000526"
     CDS             29402..30031
                     /locus_tag="ON498_000526"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR3938826.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30019..30333
                     /locus_tag="ON498_000527"
     CDS             30019..30333
                     /locus_tag="ON498_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938827.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30371..31012
                     /locus_tag="ON498_000528"
     CDS             30371..31012
                     /locus_tag="ON498_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3938828.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31055..31747
                     /locus_tag="ON498_000529"
     CDS             31055..31747
                     /locus_tag="ON498_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR3938829.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            31744..32646
                     /locus_tag="ON498_000530"
     CDS             31744..32646
                     /locus_tag="ON498_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3938830.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(32804..34510)
                     /gene="efbA"
                     /locus_tag="ON498_000531"
     CDS             complement(32804..34510)
                     /gene="efbA"
                     /locus_tag="ON498_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR3938831.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            34700..35323
                     /locus_tag="ON498_000532"
     CDS             34700..35323
                     /locus_tag="ON498_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938832.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            35773..36822
                     /locus_tag="ON498_000533"
     CDS             35773..36822
                     /locus_tag="ON498_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3938833.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            36819..37700
                     /locus_tag="ON498_000534"
     CDS             36819..37700
                     /locus_tag="ON498_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938834.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLFLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            37703..38473
                     /locus_tag="ON498_000535"
     CDS             37703..38473
                     /locus_tag="ON498_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3938835.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            38752..39606
                     /gene="aroE"
                     /locus_tag="ON498_000536"
     CDS             38752..39606
                     /gene="aroE"
                     /locus_tag="ON498_000536"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3938836.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            39784..40947
                     /locus_tag="ON498_000537"
     CDS             39784..40947
                     /locus_tag="ON498_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3938837.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41064..41474
                     /locus_tag="ON498_000538"
     CDS             41064..41474
                     /locus_tag="ON498_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3938838.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            41648..43876
                     /gene="topB"
                     /locus_tag="ON498_000539"
     CDS             41648..43876
                     /gene="topB"
                     /locus_tag="ON498_000539"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3938839.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44047..44331
                     /locus_tag="ON498_000540"
     CDS             44047..44331
                     /locus_tag="ON498_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938840.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            44491..44727
                     /locus_tag="ON498_000541"
     CDS             44491..44727
                     /locus_tag="ON498_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3938841.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            44724..45123
                     /locus_tag="ON498_000542"
                     /pseudo
     CDS             44724..45123
                     /locus_tag="ON498_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        14861 a         7860 c         9846 g        12752 t
ORIGIN      
        1 aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat
       61 caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg
      121 caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag
      181 agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa
      241 gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca
      301 attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa
      361 cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc
      421 aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg
      481 gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat
      541 cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca
      601 tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa
      661 gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca
      721 ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg
      781 atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat
      841 taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg
      901 gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc
      961 tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga
     1021 tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag
     1081 aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt
     1141 tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg
     1201 ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga
     1261 ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa
     1321 aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat
     1381 gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat
     1441 catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg
     1501 gctacgtcaa gaactaggca gtggaaatgt cattgcgaca gatatacgca tgcctgaagt
     1561 cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag
     1621 catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt
     1681 tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt
     1741 ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg
     1801 tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta
     1861 tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt
     1921 agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg
     1981 tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag
     2041 tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat
     2101 tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc
     2161 ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt
     2221 tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc
     2281 actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt
     2341 gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa
     2401 gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa
     2461 gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa
     2521 cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg
     2581 gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt
     2641 tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca
     2701 gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca
     2761 gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc
     2821 ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt
     2881 gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa
     2941 gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc
     3001 gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca
     3061 ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca
     3121 tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat
     3181 ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat
     3241 gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt
     3301 gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg
     3361 atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt
     3421 atggcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact
     3481 aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc
     3541 ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg
     3601 gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa
     3661 gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca
     3721 agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt
     3781 atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact
     3841 gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa
     3901 aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta
     3961 aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact
     4021 attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga
     4081 agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta
     4141 tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa
     4201 ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc
     4261 gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc
     4321 tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc
     4381 gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag
     4441 gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt
     4501 agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct
     4561 agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt
     4621 ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga
     4681 gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga
     4741 acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt
     4801 tttattactc ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa
     4861 aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact
     4921 ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac
     4981 attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag
     5041 aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt
     5101 ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt
     5161 ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga
     5221 agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa
     5281 aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat
     5341 gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct
     5401 tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc
     5461 tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag
     5521 aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg
     5581 ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata
     5641 atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt
     5701 ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga
     5761 ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca
     5821 aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga
     5881 aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta
     5941 tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt
     6001 cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt
     6061 agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac
     6121 cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga
     6181 agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac
     6241 tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga
     6301 aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg
     6361 taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat
     6421 cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg
     6481 cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg
     6541 acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat
     6601 cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt
     6661 tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata
     6721 ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc
     6781 aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg
     6841 agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa
     6901 aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa
     6961 agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc
     7021 acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc
     7081 tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt
     7141 atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt
     7201 ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc
     7261 ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt
     7321 cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg
     7381 ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa
     7441 aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt
     7501 tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc
     7561 gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca
     7621 gtgatcaata gaatgcgtat actaaataag tacgaaaagg atatgatttg ttttttaaga
     7681 ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt
     7741 ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt
     7801 acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt
     7861 tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt
     7921 cgacaaatgt ttcttgattt cttccaatct aaagaacata cgattgaacc tagtgcgtct
     7981 ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa
     8041 aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct
     8101 attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag
     8161 atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg
     8221 gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt
     8281 tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat
     8341 cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat
     8401 acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa
     8461 aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa
     8521 ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg
     8581 atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac
     8641 ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa
     8701 aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt
     8761 gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg
     8821 cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt
     8881 ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa
     8941 gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa
     9001 ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta
     9061 gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa
     9121 gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat
     9181 gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct
     9241 gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca
     9301 gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt
     9361 gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg
     9421 gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa
     9481 aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga
     9541 caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca
     9601 gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct
     9661 ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact
     9721 tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca
     9781 gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt
     9841 ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg
     9901 tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa
     9961 tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa
    10021 ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa
    10081 ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag
    10141 gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat
    10201 ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat
    10261 cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta
    10321 gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa
    10381 gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt
    10441 ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca
    10501 cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac
    10561 aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct
    10621 catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag
    10681 ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa
    10741 tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt
    10801 acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt taacaattat
    10861 ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg
    10921 cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga
    10981 tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt
    11041 agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc
    11101 ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt
    11161 attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac
    11221 cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac
    11281 accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc
    11341 ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc
    11401 gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta
    11461 agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag
    11521 cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc
    11581 ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt
    11641 catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc
    11701 cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt
    11761 atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct
    11821 ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag
    11881 gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa
    11941 cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat
    12001 gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt
    12061 atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc
    12121 cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc
    12181 atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag
    12241 tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact
    12301 ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg
    12361 aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc
    12421 ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg
    12481 tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag
    12541 aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt
    12601 aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt
    12661 taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg
    12721 caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta
    12781 gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct
    12841 gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt
    12901 ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag
    12961 ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc
    13021 ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa
    13081 ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa
    13141 aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc
    13201 attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat
    13261 tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta
    13321 aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc
    13381 ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag
    13441 aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt
    13501 gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc
    13561 taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac
    13621 gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta
    13681 tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg
    13741 atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc
    13801 gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt
    13861 ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct
    13921 gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta
    13981 attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat
    14041 attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc
    14101 acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga
    14161 cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc
    14221 aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg
    14281 cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa
    14341 atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc
    14401 tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg
    14461 tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat
    14521 tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga
    14581 tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt
    14641 tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct
    14701 tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt
    14761 tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct
    14821 gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca
    14881 cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc
    14941 attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga
    15001 gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt
    15061 cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga
    15121 gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga
    15181 acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga
    15241 actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt
    15301 atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga
    15361 tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac
    15421 agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca
    15481 aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa
    15541 aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat
    15601 cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga
    15661 tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa
    15721 tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt
    15781 attttgacta cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc
    15841 attctttttc tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa
    15901 acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc
    15961 aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg
    16021 ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa
    16081 gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta
    16141 tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca
    16201 gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag
    16261 gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat
    16321 gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc
    16381 tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt
    16441 aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga
    16501 ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct
    16561 gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt
    16621 aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt
    16681 agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc
    16741 ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa
    16801 ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca
    16861 agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg
    16921 gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta
    16981 tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca
    17041 ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc
    17101 atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta
    17161 tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag
    17221 gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta
    17281 tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc
    17341 cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata
    17401 ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca
    17461 tgaatctggt aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca
    17521 aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa
    17581 ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga
    17641 tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat
    17701 aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt
    17761 caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg
    17821 tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca
    17881 ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc
    17941 tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc
    18001 gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt
    18061 tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag
    18121 aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt
    18181 atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg
    18241 ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt
    18301 tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag
    18361 taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct
    18421 aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag
    18481 tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc
    18541 aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga
    18601 tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca
    18661 tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag
    18721 aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat
    18781 cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga
    18841 catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg
    18901 acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc
    18961 attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg
    19021 gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg
    19081 acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga
    19141 aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac
    19201 attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc
    19261 ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg
    19321 tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca
    19381 ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag
    19441 tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat
    19501 ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg
    19561 gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt
    19621 atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag
    19681 gttttctcgg attgattccg attttactag gtattattag cggatacatc gtagccttgc
    19741 tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact
    19801 ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg
    19861 cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac
    19921 taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat
    19981 tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca
    20041 ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat
    20101 ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac
    20161 cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga
    20221 ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta
    20281 tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa
    20341 tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa
    20401 gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc
    20461 atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc
    20521 aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc
    20581 tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag
    20641 ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat
    20701 acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg
    20761 atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg
    20821 gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg
    20881 aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc
    20941 gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag
    21001 gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg
    21061 aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg
    21121 gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg
    21181 cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag
    21241 aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg
    21301 gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg
    21361 aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga
    21421 cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct
    21481 cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag
    21541 tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg
    21601 ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc
    21661 ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag
    21721 ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg
    21781 atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc
    21841 aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag
    21901 tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg
    21961 cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag
    22021 atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag
    22081 aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt
    22141 caccccatca tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa
    22201 tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg
    22261 gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa
    22321 gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata
    22381 ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta
    22441 aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg
    22501 tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag
    22561 gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca
    22621 acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa
    22681 gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc
    22741 gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt
    22801 caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat
    22861 gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca
    22921 aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata
    22981 tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct
    23041 agtatagttg atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt
    23101 ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca
    23161 ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt
    23221 aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg
    23281 ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa
    23341 gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt
    23401 caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga
    23461 ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat
    23521 catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta
    23581 gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg
    23641 cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt
    23701 atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa
    23761 gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta
    23821 cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa
    23881 ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc
    23941 gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc
    24001 aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt
    24061 agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga
    24121 tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat
    24181 cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt
    24241 tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt
    24301 acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg
    24361 aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc
    24421 aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat
    24481 cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc
    24541 tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga
    24601 tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc
    24661 gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg
    24721 attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc
    24781 tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg
    24841 agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa
    24901 tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga
    24961 acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca
    25021 agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt
    25081 agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat
    25141 cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa
    25201 cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca
    25261 gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga
    25321 attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg
    25381 atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt
    25441 tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc
    25501 aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt
    25561 atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat
    25621 aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc
    25681 tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga
    25741 tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac
    25801 aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc
    25861 aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga
    25921 ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt
    25981 gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg
    26041 gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc
    26101 acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc
    26161 attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt
    26221 ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac
    26281 aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact
    26341 tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt
    26401 ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg
    26461 agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc
    26521 tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga
    26581 agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa
    26641 acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc
    26701 tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt
    26761 tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag
    26821 agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt
    26881 tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa
    26941 gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc
    27001 gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta
    27061 aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta
    27121 ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt
    27181 tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta
    27241 gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa
    27301 gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta
    27361 gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat
    27421 tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac
    27481 ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt
    27541 tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa
    27601 aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc
    27661 gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga
    27721 ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc
    27781 ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag
    27841 aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc
    27901 caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga
    27961 atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat
    28021 tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact
    28081 atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca
    28141 tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt
    28201 ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc
    28261 caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt
    28321 ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt
    28381 tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga
    28441 ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag
    28501 tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga
    28561 atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg
    28621 aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat
    28681 gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga
    28741 agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt
    28801 gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt
    28861 tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc
    28921 aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc
    28981 ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc
    29041 tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt
    29101 ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg
    29161 tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt
    29221 ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt
    29281 cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac
    29341 tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa
    29401 aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa
    29461 tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg
    29521 aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat
    29581 caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca
    29641 tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa
    29701 agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt
    29761 gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag
    29821 agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa
    29881 agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat
    29941 tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg
    30001 gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg atgaagatag agaaatccga
    30061 tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc
    30121 atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa
    30181 ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt
    30241 atatatagat tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga
    30301 aatgttgcga atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga
    30361 ggtaactttt atggggttga atgaatttat tttaggattg aacaatttag agacgatcac
    30421 aagggcacct ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt
    30481 agcatctatt gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg
    30541 gaaactgctc tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat
    30601 caaaacgccg gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga
    30661 aaagatgacg gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg
    30721 ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga
    30781 taaggttgac cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt
    30841 ttataaggag atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc
    30901 tgttcagtat ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa
    30961 agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata
    31021 tgagatgcag ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg
    31081 agacaaaggt ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt
    31141 tccattgcag tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac
    31201 gctttccttt cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca
    31261 tttagtacct tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac
    31321 agatattcat ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga
    31381 atttcatttt gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca
    31441 gctaatggta caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga
    31501 aaaacatgag cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca
    31561 ttatattggc tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga
    31621 tcatgtagga aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc
    31681 gaacaaccga ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga
    31741 gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga
    31801 gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta
    31861 gaaaccgagt tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa
    31921 gaagggcaag acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa
    31981 actcactatc taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat
    32041 ttcttaggtc ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt
    32101 cggatcatgg agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag
    32161 atcggcatta tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa
    32221 ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga
    32281 catccattag ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt
    32341 cgttttacac aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct
    32401 gatcaagaaa cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa
    32461 actgatgctt acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg
    32521 ttaatccctt tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt
    32581 ccgctcagtg aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag
    32641 agctaagaaa agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt
    32701 tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg
    32761 ttgtgggaaa gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca
    32821 tagctgtaat tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa
    32881 cataaccagg cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg
    32941 gcacttgggc agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa
    33001 ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg
    33061 catgaagcca atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat
    33121 tgtttttacc aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg
    33181 gtttcttacg tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt
    33241 cttctttaat tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt
    33301 caagatattg gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt
    33361 tcttcagctt ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg
    33421 gatctagtgc tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt
    33481 ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat
    33541 tctcagcgtt ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc
    33601 gttttagttc attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt
    33661 tctctgcttt ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta
    33721 cagcagatgc ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt
    33781 ccgtttcgta aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata
    33841 caaccatctt ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga
    33901 aaccgattcc ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc
    33961 gctgaaagat tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag
    34021 caacatattc tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt
    34081 tgatcgcatc cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc
    34141 ccattaattc aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa
    34201 agtggaaatg gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat
    34261 ccaaatattt tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga
    34321 tttgggtaat ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt
    34381 tgccgcgtga acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga
    34441 tccggccaga tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat
    34501 caaaggacat ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa
    34561 gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt
    34621 acataaaaaa cagtgtttct atcgttgaaa tccaaatttt taggtataat aaaaaatagg
    34681 agaaaaagta gaaaagaagg tgtttgttat ttgggaagta gttaaatcga gagtacaaga
    34741 aatgaatgaa catttctcag tgtcattcgc agaaaatcca agtaagtatt ttcgacgtct
    34801 tgtttttctg ctcatcttat cattgctgtt aacagttgca atgtttttat tgccggaaaa
    34861 aatccgagaa gagcaagtaa atagtctcgt ttattcgtcc ataccaacca gcaaattgaa
    34921 accttttgat tacagcaaag ctgatcagaa aatcaaacaa agccgggcga tatcggtgat
    34981 gttcagcaaa ccgaacggga aaacctatca ggacatgctt gatgttttta atgatcctga
    35041 gcttatggaa gaattgaatc gaccgatctt ttattatccg atcgtttatg atgtacatga
    35101 actggaacaa aaatataaca tacgaacgga tgaagtgaca tttattttct ttgatggagg
    35161 aaaagaagca aatcggataa ctgcgggtaa gggaggaata actgattttg ataaggagtt
    35221 gatccctgaa ctgaatcgac ttccgttagc caatatcaaa caattagaag aagaattgga
    35281 tagaacggga gtaacttccc aaaattccga gattgctaat tgagacattg gcgcgatgaa
    35341 acggtgggtc tatatagcgc tatttttaat tatcagaaca agtgatgttt gatcttcaaa
    35401 ataacaaata aaaaattaaa aaatatagtt gacgaaattc aaaaattcga ttagtcttaa
    35461 atcaattctt aaggaaaggg tgacctgtaa tgaacttatc aaaacgtatt tcagcagaaa
    35521 acaaaaaaaa ttggcgcagc tggcgtagat aagttgtatt aatggtgaga ctttcccata
    35581 gatacaactt tttagagtgc atttctaaag cttgtatcta tgctagacat gaacattgac
    35641 tgccgcatag attagaatct atgcggtttt ttgtgtaaag acaatgaccg ctttggccat
    35701 tgtctttttt tgtagactgg atacttgcct gattataata aataaaaaga ttgactttgc
    35761 aggaggaaaa aaatgaaaaa caaacgatta ataacagttg tagtaattat tttagtttat
    35821 ttagcaggtg catttattta tggaaaagcc tcaggcgaaa aagaaagtac ttcgacgaac
    35881 cagacaaaaa aaacaacagt tgttggggta ttgcaatacg tgagccatcc ggcattagat
    35941 gagatttatc gaggaatcaa agatggtctg aaagaagaag gattaaaaga aggagaaaat
    36001 cttgagatca aatttcaaaa tggacaagcc gatcagagca agctagccac aatgagtcaa
    36061 cagctagtac aatctgatcc agatgccttg attggaatcg caacacctgc cgctcaatca
    36121 ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg ctgtaacgga tcctgtgggg
    36181 gctggattag tcgcttcttt agagaaacct ggtggtaatg taacaggtgt ttctgatcag
    36241 ccgcctgtta cttcacaaat caagcttgct tctgatttgt tgccaaatgc aaaaaaagtc
    36301 ggaatcttgt atgcttcttc agaggataat tcaaaatacc aagtagcaca agcagaagaa
    36361 gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg tgccttccac taatgagatt
    36421 gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta tctatgttcc attagataat
    36481 accattgcca atgcgatgca aacagtggta aaagaagcaa acaaagcaaa tatccccgtc
    36541 attccatctg tggatacaat ggtggaacaa ggtggactag caacaatcgg gatcaatcaa
    36601 tatcaattag ggcttcaaag cggtaagatg gcggcagaac ttgcgagtgg aaaagaaaaa
    36661 ccggaaacga cgcctgttta tacatttgat caaggggata cagtgatcaa tcaatctcaa
    36721 gctgatcgct taggaatcat tgttccccaa tcgatgaagg aaaaagccaa aatcattaca
    36781 gatgactcgc agcaagaaac atctaaggag gatgacaaat gatcgtatca gctatttcac
    36841 aaggcatgct ttgggcaata ttaggattag ggattttcat gacttatcgg atcttagact
    36901 ttcctgatat gacgacagaa gggtccttcc cattaggtgc tgctgtctgt gtgacagcta
    36961 ttactcatgg ggtagcgccg attgtagcta cattattagg cgtaggtgct ggcatgatag
    37021 caggactcgt tacggggtta ctttatacaa aaggaaagat accggttatt ttagcaggga
    37081 ttttagtgat gtcaggattg aactcagtta ttttgtttgt gatgcgaaca ccaaatcttt
    37141 ttttattgaa tcatccgatt ctacaagaca gcttccatcg ctttgggtta cctgattact
    37201 ttgacacagt ccttcttggc ttcatctctt tgggcatctt gattgctgta ctgctattct
    37261 tcttcaatac agatttgggt caaggctata ttgcgacagg agacaacgaa acgatggctc
    37321 ggtcgttagg gataaaaacc gatcgtatga aaattttagg tttaactttg tcgaacggtg
    37381 tcattgcatt atccggtgcg ctgattgccc agaacgacgg atatgcagat gtaaataaag
    37441 ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat cggtgaagtg atcttccaag
    37501 aattgacgct agcagaaaga ttgattgcta ttgttgtcgg cagtatcatt tatcagctat
    37561 tgattttagt agtaatcaaa ctagggtttg acacgactta tctgaagttg ttctccgcta
    37621 tcatcttagc tatttgtctg atgatcccgc aattgaagaa agcactgaac ttaaaaacgg
    37681 gttttgagaa ggaggcatag taatgactga tatattgaca atcaaaaatg gcagaaaagt
    37741 cgtaaacaat ggaatgaatg aagagaaagt actattggat gaaattaacc ttacaatcag
    37801 agaaggggac ttcatcactg tccttggcgg gaatggtgct ggaaaaagca cactgttcaa
    37861 tacgatcgct ggtacgcttc aactcagtca aggatcgatc tcttttaaag atcagctgat
    37921 cacaaaagat tcagaagaaa aaagagcacg attcctttct cgagttttcc aagaccccaa
    37981 gatgggaacg gctcctcgga tgacagtagc agaaaatttg ctacttgcac aaaaaagagg
    38041 tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa aaagaagatt ttttcagact
    38101 atgtcaagaa gtcggtaatg gcctagagca acacttggat actccagcgg gtgaattatc
    38161 tggtggtcaa cgccaagcat taagcttgct gatggcgacg atcacagctc ctgagttact
    38221 attattagat gaacatactg cagctttaga tcctaaaaca gctaaagcat taatgcgatt
    38281 aacaaatcag cggatcaatg aacaaaaatt gacttgtctc atgatcacgc atcgaatgga
    38341 agacgccttg aaatacggaa atcgtctgat cgtactccaa aaagggaaaa tcgttcaaga
    38401 tatttctgaa aaagaaaagc aacatttgac aatgggcgac cttttgcagt tctttgaaga
    38461 agtagtggat tgatttcgac agaaaaattc ctttcagtag aaggacttga aaactcattg
    38521 acatcttcct ttgattcatc ctatacttaa aacaataaac aattaaacat atttgggtgt
    38581 tttgtaggat cacgtagtca atgattttaa caattaaata gaatattctt tgctctgaca
    38641 agtaaacgat gagaaaagaa tttgctactt tgccacctag acgtttcgta ctttttcgta
    38701 tcaaacgcta agtggctttt tttattgaag taagaacaaa ggaggaataa gatgatttct
    38761 ggacaaacca aattagctgg gctctttgct gatccggctt ctcacagttt atccccattg
    38821 atgcacaata ctgcttttga agcaaacaac atcgatgcgg tgtatttagc tttccaagta
    38881 aatccaatga atttgaagca agcagtcgaa agtattcgaa cttttgatat gttaggtgta
    38941 aatctttcga tgccgaataa aacagcggtt attccttatc tggatgaggt aagccaggaa
    39001 gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa agggtcgttt gattggctat
    39061 aatactgatg gtgcgggctt catgcaatcc gtgcaagaag ctgggatttc gatcaaaaag
    39121 aaaaaaatga ctctactggg agctggaggt gcagcaaagg cagtcactgt tcaagcagcg
    39181 ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca accaaacatt cgctcaagta
    39241 aaagaaacat tcgaacaact cagctatcga acttcttgta agataaaagt tcgtgacttt
    39301 gccgatacgc atcaattaag aaaagacttg aaagaaagct gtcttctagt caatgcaaca
    39361 gatatcggga tgggaaagaa aatcaatcag tcaccgctct tagatataag catgatgcat
    39421 gagaatctag cggtttttga tttgatttat gcacctagag aaacacgttt attaaaagaa
    39481 gcaaaaaaag taggagcaaa aggatacaat ggacttggca tgttgatcta ccaaggagca
    39541 atcgcgtttg aattatggac aaaggaaaag atgccgatcc atgtgattga aaagctgttt
    39601 gattaaaaaa ggataataga taaagatctt ttattctcgc ataaacggtc tcaagaaaag
    39661 taatccttta tgtatcgaag catttatttc gttatttttt attaaattat caaaaaatga
    39721 taggcttttg tcgattggtt atcgtatcct ttatttatca gtaagaaacg aggaggaacc
    39781 acaatgagtc ggatggatcg ctataaggac atccataaga aggcgaaacc tctgaagcgg
    39841 gatacgggga gtatttttgc tagaagagaa cgaaaacgaa cgcgtatgga aaatgaggaa
    39901 gatgctaccc gtatttatga aagagagcag cattcatttg aacatttgaa ccaatacgag
    39961 caacagatat acagagatgc accaagagca gaagaacaac aaaaaaataa aaaatttttc
    40021 aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa agcgatcgtg gaaaaaaatc
    40081 attctatcga ttttgcttgt tttgatcctt tattcaggta tctcattttt tattggtaaa
    40141 tatgtagctg aacatgacac aagcttagag gcagcgactt cagaaacctt tcatgggcaa
    40201 aaagcgacga atggtgcgca taatattttg atccttggaa gtgatacacg cggagaagat
    40261 gccgggcgag ccgatacgat catggtctta cagttggatg gtccttccca taagccgaaa
    40321 ctgatttctt ttatgcgtga cagcttcgtt acgatcccag gggtaggaca aaataagatc
    40381 aattcagctt atgcatacgg aggagccgat ctagtgcgtc aaactttagt agaaaatttt
    40441 ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat cgtttgaaaa agtgatcgat
    40501 gccttattca tgaacggagt gaagatcgat gcagaaaaag atctgaatct ggatggtgta
    40561 gatattaaaa aagggggcca gaagatggat ggacatgttc ttttgcagta cgcacgtttc
    40621 cgaatggatg aacaaggaga ctttggccgt gtcagacgcc aacagcaagt aatgaacgct
    40681 attttcagcc aattgaagaa tccattgaat ctgatccgtg caccttatgc agctggtaaa
    40741 gctttaggat atacatccac agatgtcact gcttggttct tgacgaaaaa cctcttttcg
    40801 attgcacgag gagctggtgg cattgatcga ataagtgtgc cagtagacgg ttcgtggaat
    40861 tatgggcaaa gtgattatgc tggcagtatt cttgaaattg ataatcaaat gaatcaatct
    40921 gctattacag attttttgaa taaatgatca taaaaaagat caagaagaag ctgcgcaagg
    40981 cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa cgctttctta tggtagattt
    41041 aaccaagaga ggagctgttt acattggata tctatctgaa cttcaaaaat gagtcgcgtg
    41101 aagccatcgc tttttatgaa aaagtttttc agactgaagc atcaggtatc accacatacg
    41161 gtgaaatgcc tggaatagaa caaatgaact tgccagaaga agcaaaaaat ctaatcacga
    41221 atgcacggct gaacatttat gatacattag ttatgtttgc agatacgcca gaaggaattg
    41281 gtcctgaatt agttgtggga aataatgttt ctttagtcat ccagacaaga aatgaagaag
    41341 aaacgaagcg cctatttgaa agcttagctg agggtggtaa gattgagatg ccactggatg
    41401 ctgttccgtg ggctcagttg tatgggatgg taactgatcg atttggcatc aactggcaga
    41461 ttaaccaaga gtgacagcta aagatgaata ttttctggaa acatcaagca gcaaacggat
    41521 catcgtttgt ttgcttgatg tttttgtgtc tatttcgact atacggtatc ttttttttat
    41581 tgccagatgc gaaaatatgt tccataatag agaagtccgc aaaaaattaa gtaacggagg
    41641 tcagaagatg aagacggtga tactagctga aaaaccttca caagcaaaag cctatgccga
    41701 aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa atcagcgatc cattgtttgc
    41761 tggtgaagta accatcactt atggttttgg acatttggta gatatggttg ctccaggagc
    41821 ttatgaagag cgttgggcaa aatggtcttt agaaaatttg cctattttcc cagaaacctt
    41881 ccgttacgaa gttccaatgg acaaacgtgc acaattttcg atcgtaaaac gagaattgca
    41941 gtcagccgat accatcatta ttgcaacaga tggtgatcga gaaggtgaag ccatcgcgtg
    42001 gtcgattatt cgacaagcaa acgcattttc taaggaaaag aactatttac gtttatggat
    42061 caattctctt gaaaaagaag caatctatga aggttttaag aatctccaac ctggaaagaa
    42121 ctattttcca aaatataaag aagcgcaagc aaggcagaac gctgattggc tgattggaat
    42181 gaatggaagt ccattatata gcttgctact tcaacgaaaa gggattgatg gcagtttttc
    42241 tcttggtcgt gtacaaacac cgacactcta tatgatttat cagctgcaag aagaaatcaa
    42301 acacttcaaa aaagaaacgt actttgaagg agaaggttta attacgacaa acaaagggca
    42361 gttttctgga aaaatcgtac caaaaaaagc atttaaaacc caagaagagt taacgaagga
    42421 aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga cggattcttg aagttacgaa
    42481 aaaggaaaag aggttgcata gtccacgttt attttcgcta tctagtttgc agacaaagat
    42541 gaatcagcta atgaaagcaa gtgccaaagc tacacttgat gctgctcaag gattgtacga
    42601 aagcaagttt ctaagctatc caagaacgga ttccttttac attacagaaa atgagcatca
    42661 gtatctggtt aaacatctgc aaaattataa aaaattttta ggaatagaag aagtacagac
    42721 aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa aaagttcaag aacatcatgc
    42781 aattattccg acaaagacga ttccgactcc tgcacgcttt gcgaagcttt ccaaattaca
    42841 gcaagcaatc tatttgcaag ttttgcgcac tactgtggct atgtttgctg cagattatat
    42901 ctacgaagag acgaccgtaa tgacgggcgt acagcaatta aagctaaaaa gcatcggaaa
    42961 gatcccatta cagcaaggtt ggcaggaaat cttacgcatc aagtcaaaaa agaaagaagc
    43021 tcagactctt cctcctatcg tagaaaatga agaagcagaa gtagaactga aaagtatgga
    43081 gaaagaaaca caacctccaa aaccctatac cgaaggaacg ttgatcacag caatgaaaac
    43141 tgcgggaaaa acgctggata atgatgaagc gcaagaaatt ctcaaagaag tagaaggaat
    43201 agggacagaa gctacacgag ccaatatcat tgaaaatctt aagcagaagc attacatcga
    43261 agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc actctttgta aggcagtagc
    43321 caatgaacca ttactgacga gcgcagaaat gactgcgcgc tgggaagggt acttgagaaa
    43381 aattggtaaa caagagggga caccatctgt ttttttagag aatatcaaaa aattcatcct
    43441 acatttattg gcagaagttc ctaatcaaat cgacaaggta gacttttcag atgagattag
    43501 cgggacacgt cagataaaag cgatggagaa gaaaaacgat caattaggaa gatgtccaaa
    43561 atgtaaaaag ggaatagtca tgctttatcc taaaatcgct acatgcttga atccggaatg
    43621 cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg actaaaacga atctcagaga
    43681 tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt tttactggta aaaaagggaa
    43741 attcgatgca gttttagtat taaaagaaga tatgacaatc ggtttttctt ttccatggat
    43801 cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa acatcaagaa aaaataatga
    43861 aaacgaagta gagtaaatga aaacaatgaa atagcggaat atttattccg aaaagtgaga
    43921 atataaatac caataaaact aaaaaatatc agtgaatcaa gcaaaaacgt tggggtaggc
    43981 tgattttata tagatagcaa ggagtttttt tatttgaaaa aagtgcattc atttttaaat
    44041 tatcttatgg gagcaatcac gattgtggct gtgtaccaag catttctaaa gtatcctgat
    44101 tacgggacga agttgttgtc attgacgcac aatcaaacag catattcacc aacgttgatt
    44161 ttatcgactt tgactgcagg agctaaaagc gctacttatt tttttcataa aacgacaagc
    44221 atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg tggtagcggc tatgatcatt
    44281 atttattttc taccagttat tacacaagta tttcatcaat attttggtta agaatacgag
    44341 agtttgtgac aaaagtcatg tcacaaactt ttttctttgt cattttttcg taagaaaaag
    44401 caaaataaaa gtatatacat tgattaaaag tatatacttg gatataatgt acttactata
    44461 gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa tgaaaaagta taaaattaga
    44521 aaatctggca atagcgatgt aacaactatc ccaccagaag taaaagaatt catgggtgtg
    44581 cagacaggtg acgcaatcag ttatgttttt caatctgatg gttctgtacg aatgatcaaa
    44641 gcacaagaag aacctgatat tgattcatta gttgattcga tcatgaatca atatgaagac
    44701 gcgttaaaag atttggttga tttatgaggt atctttcgac taaagaaatt atcaaaatca
    44761 acgcgaaagt tattttaccg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    44821 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct
    44881 gaaatctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    44941 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    45001 ccagttgctt tttctcgtca agaagctgtt gattttatca taatgattac cactagttca
    45061 ttggatttcg attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa
    45121 tgaacgaatt aaaaacttta tttctcaatt agatcagcat gtcattaagg aatatgctga
    45181 tttttttatg tttaatcata caaaatagtt ttttcataaa aatatggaaa atatggatta
    45241 attttgaaat aattgattta aaaggaatac aaaagagtga atagtaggaa atagtggttt
    45301 agaactagtg ggctgattt
//
LOCUS       DAJSVD010000012        42819 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSVD010000012 DAJSVD010000000
VERSION     DAJSVD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.012"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            117..971
                     /locus_tag="ON498_000543"
     CDS             117..971
                     /locus_tag="ON498_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR3938842.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="ON498_000544"
     CDS             972..2309
                     /locus_tag="ON498_000544"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3938843.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="ON498_000545"
     CDS             2314..2538
                     /locus_tag="ON498_000545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3938844.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="ON498_000546"
     CDS             2540..3430
                     /locus_tag="ON498_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3938845.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="ON498_000547"
     CDS             3439..4257
                     /locus_tag="ON498_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR3938846.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="ON498_000548"
     CDS             4364..4813
                     /locus_tag="ON498_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3938847.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="ON498_000549"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="ON498_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3938848.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="ON498_000550"
     CDS             complement(6568..6684)
                     /locus_tag="ON498_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR3938849.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="ON498_000551"
     CDS             complement(6757..7713)
                     /locus_tag="ON498_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3938850.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="ON498_000552"
     CDS             7884..8252
                     /locus_tag="ON498_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR3938851.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="ON498_000553"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="ON498_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3938852.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="ON498_000554"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="ON498_000554"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3938853.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="ON498_000555"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="ON498_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3938854.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="ON498_000556"
     CDS             10240..12432
                     /locus_tag="ON498_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3938855.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="ON498_000557"
     CDS             12458..13420
                     /locus_tag="ON498_000557"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3938856.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="ON498_000558"
     CDS             13434..14795
                     /locus_tag="ON498_000558"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3938857.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="ON498_000559"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="ON498_000559"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3938858.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="ON498_000560"
     CDS             15924..17144
                     /locus_tag="ON498_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR3938859.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="ON498_000561"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="ON498_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3938860.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="ON498_000562"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="ON498_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3938861.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="ON498_000563"
     CDS             19906..20583
                     /locus_tag="ON498_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR3938862.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="ON498_000564"
     CDS             20597..21196
                     /locus_tag="ON498_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR3938863.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="ON498_000565"
     CDS             21211..21498
                     /locus_tag="ON498_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR3938864.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="ON498_000566"
     CDS             21547..22329
                     /locus_tag="ON498_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR3938865.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="ON498_000567"
     CDS             22350..23057
                     /locus_tag="ON498_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR3938866.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="ON498_000568"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="ON498_000568"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3938867.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="ON498_000569"
     CDS             complement(26166..27692)
                     /locus_tag="ON498_000569"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3938868.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="ON498_000570"
     CDS             complement(27707..28354)
                     /locus_tag="ON498_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR3938869.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="ON498_000571"
     CDS             complement(28538..28867)
                     /locus_tag="ON498_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR3938870.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="ON498_000572"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="ON498_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3938871.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="ON498_000573"
     CDS             29788..30801
                     /locus_tag="ON498_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3938872.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="ON498_000574"
     CDS             30801..32078
                     /locus_tag="ON498_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3938873.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="ON498_000575"
     CDS             32141..34843
                     /locus_tag="ON498_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3938874.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="ON498_000576"
     CDS             34995..35312
                     /locus_tag="ON498_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3938875.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="ON498_000577"
     CDS             35342..35662
                     /locus_tag="ON498_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3938876.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35770..37230
                     /locus_tag="ON498_000578"
     CDS             35770..37230
                     /locus_tag="ON498_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3938877.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37298..37519
                     /locus_tag="ON498_000579"
     CDS             37298..37519
                     /locus_tag="ON498_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938878.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37550..37732
                     /locus_tag="ON498_000580"
     CDS             37550..37732
                     /locus_tag="ON498_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR3938879.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37732..38145
                     /locus_tag="ON498_000581"
     CDS             37732..38145
                     /locus_tag="ON498_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR3938880.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38268..39449
                     /locus_tag="ON498_000582"
     CDS             38268..39449
                     /locus_tag="ON498_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938881.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(39520..39684)
                     /locus_tag="ON498_000583"
     CDS             complement(39520..39684)
                     /locus_tag="ON498_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938882.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            39838..39911
                     /locus_tag="ON498_000584"
     tRNA            39838..39911
                     /locus_tag="ON498_000584"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39872..39874,aa:Arg,seq:cct)
     gene            complement(39980..41119)
                     /locus_tag="ON498_000585"
     CDS             complement(39980..41119)
                     /locus_tag="ON498_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3938883.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41418..42053)
                     /locus_tag="ON498_000586"
     CDS             complement(41418..42053)
                     /locus_tag="ON498_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938884.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42166..42801)
                     /locus_tag="ON498_000587"
     CDS             complement(42166..42801)
                     /locus_tag="ON498_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCR3938885.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        14194 a         7291 c         9010 g        12324 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgtttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt
    35701 gaacggattt tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg
    35761 aagtaaaaaa tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca
    35821 aaaatcggag cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg cacactgccc
    35881 gcaacaatgg cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa
    35941 cagtttaaca gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg
    36001 aatgtcatca tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg
    36061 atctttgcct tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct
    36121 ggggggatcg ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca
    36181 gcagcattag atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt
    36241 ggtgcaacga ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat
    36301 catttgaatg gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt
    36361 ttttgtaaat tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca
    36421 gtatctaaag catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt
    36481 atcaattata tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa
    36541 tatattgcag aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca
    36601 gtatttgttc aagtattctg gttctttggt attcatggac caaatgtatt ggcaccagtg
    36661 ctagagggaa tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat
    36721 aacggaaaaa ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg
    36781 tgggtccgcg gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc
    36841 ttactgatag ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta
    36901 tccattgggc ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta
    36961 ttgaatgcaa tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga
    37021 tggacagcta cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta
    37081 ccgccgttct tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca
    37141 ttagtatgta tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa
    37201 atggaccctg ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt
    37261 ttcgttcctc ataatgaatt ttctaggagg aactgaaatg attacaattg ccagtgcaat
    37321 gcaacaagat gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac
    37381 atttaaagaa aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt
    37441 tgcggctaaa aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt
    37501 tcaatcaaca gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa
    37561 cggtttattt ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc
    37621 gcaaaggtat gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta
    37681 tttcttcatg aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc
    37741 acgaaaaaat taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt
    37801 ttcgatgtac gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag
    37861 tacgtcaaac aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa
    37921 aaccagtctt ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa
    37981 atcaaaccat tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat
    38041 ggcatgcttc aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg
    38101 agacgtttca aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg
    38161 tactatttag ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat
    38221 ctttcctgcg aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag
    38281 taagagagct aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta
    38341 atggggcaga tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga
    38401 attacggatt ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc
    38461 cagtgaattt ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc
    38521 cagaagcaag gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc
    38581 gtgatcgtaa agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct
    38641 atggctatga acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt
    38701 ttatcaaaaa aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa
    38761 tcagaagaat ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact
    38821 ggtggtatac gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg
    38881 ataatgagga agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg
    38941 cggagtgggg atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc
    39001 acaatggtgc ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt
    39061 atctattaga aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg
    39121 tagatatttc tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc
    39181 tacaaataga agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg
    39241 aaaatggaaa aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa
    39301 gtatccaaca tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc
    39361 aagaaagaat acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc
    39421 aacagccaat tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca
    39481 ccaccctttt gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt
    39541 cgtaaatttg aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat
    39601 tgcttctttc gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac
    39661 tttttctttt ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc
    39721 tagtttatca tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt
    39781 ttagcagata agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt
    39841 cctgtagctc agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc
    39901 agtcaggaac gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt
    39961 gtgccaaaaa tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga
    40021 ttttttcttt atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag
    40081 tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa
    40141 gtaaaataga tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta
    40201 attcttttaa ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt
    40261 ttaagttgaa aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt
    40321 tttgattgat tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc
    40381 tatttgaagt ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat
    40441 taatagtgat gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag
    40501 caaatcgcat tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag
    40561 aaaggttttc ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat
    40621 cttcttttgg cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg
    40681 gaattaatcc ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa
    40741 taacggtaga ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc
    40801 tatctaaatc agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt
    40861 cgtattttct ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct
    40921 caaagtaatc tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat
    40981 attctaattc gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta
    41041 tcttcgtgta ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac
    41101 gtttgactaa tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata
    41161 aagaagccca ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa
    41221 ggatgtgcta tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga
    41281 ggtgtttatt atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt
    41341 atttgttgat tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt
    41401 catattagaa agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat
    41461 attttgtagc ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt
    41521 tctaacagac caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag
    41581 ccagacatgc tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac
    41641 aattgaatct caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc
    41701 catgaagcgc tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt
    41761 ttattttgtg cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt
    41821 tggtccttgt tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat
    41881 gattcgttta aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac
    41941 ccaaaataaa gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca
    42001 actattaatt caagatattc ttttttggtt agtttttcat tgctattatc catagttacc
    42061 tcctattttg tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg
    42121 ttttattatt gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac
    42181 tttactatta atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact
    42241 aatttctcct gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc
    42301 cttcgcaaaa attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc
    42361 atcgtactct ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc
    42421 ttcaaaagta tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc
    42481 tggagtaata gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa
    42541 aaattcttca aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc
    42601 agatgatgag ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa
    42661 acctacggta gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt
    42721 ttttttgata aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt
    42781 aaaaccaaat ataccgtcca ttaatagatc ctctttctc
//
LOCUS       DAJSVD010000013        38484 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSVD010000013 DAJSVD010000000
VERSION     DAJSVD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.013"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(148..2301)
                     /locus_tag="ON498_000588"
     CDS             complement(148..2301)
                     /locus_tag="ON498_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR3938886.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTSAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            2599..3771
                     /locus_tag="ON498_000589"
     CDS             2599..3771
                     /locus_tag="ON498_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR3938887.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            complement(3927..4775)
                     /locus_tag="ON498_000590"
     CDS             complement(3927..4775)
                     /locus_tag="ON498_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3938888.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(4999..6939)
                     /gene="thrS"
                     /locus_tag="ON498_000591"
     CDS             complement(4999..6939)
                     /gene="thrS"
                     /locus_tag="ON498_000591"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3938889.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(7276..7455)
                     /locus_tag="ON498_000592"
     CDS             complement(7276..7455)
                     /locus_tag="ON498_000592"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR3938890.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(7502..9325)
                     /gene="cadA"
                     /locus_tag="ON498_000593"
     CDS             complement(7502..9325)
                     /gene="cadA"
                     /locus_tag="ON498_000593"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3938891.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            9603..11882
                     /locus_tag="ON498_000594"
     CDS             9603..11882
                     /locus_tag="ON498_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3938892.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(11975..12622)
                     /locus_tag="ON498_000595"
     CDS             complement(11975..12622)
                     /locus_tag="ON498_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938893.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(12801..13520)
                     /locus_tag="ON498_000596"
     CDS             complement(12801..13520)
                     /locus_tag="ON498_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3938894.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(13651..14802)
                     /locus_tag="ON498_000597"
     CDS             complement(13651..14802)
                     /locus_tag="ON498_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR3938895.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(15019..15759)
                     /locus_tag="ON498_000598"
     CDS             complement(15019..15759)
                     /locus_tag="ON498_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3938896.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(15763..16086)
                     /gene="rsfS"
                     /locus_tag="ON498_000599"
     CDS             complement(15763..16086)
                     /gene="rsfS"
                     /locus_tag="ON498_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3938897.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(16143..16736)
                     /gene="yqeK"
                     /locus_tag="ON498_000600"
     CDS             complement(16143..16736)
                     /gene="yqeK"
                     /locus_tag="ON498_000600"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR3938898.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(16720..17370)
                     /locus_tag="ON498_000601"
     CDS             complement(16720..17370)
                     /locus_tag="ON498_000601"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3938899.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(17380..17691)
                     /gene="yhbY"
                     /locus_tag="ON498_000602"
     CDS             complement(17380..17691)
                     /gene="yhbY"
                     /locus_tag="ON498_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3938900.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(17707..18816)
                     /gene="yqeH"
                     /locus_tag="ON498_000603"
     CDS             complement(17707..18816)
                     /gene="yqeH"
                     /locus_tag="ON498_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR3938901.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(18813..19340)
                     /locus_tag="ON498_000604"
     CDS             complement(18813..19340)
                     /locus_tag="ON498_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR3938902.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(19520..20305)
                     /locus_tag="ON498_000605"
     CDS             complement(19520..20305)
                     /locus_tag="ON498_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3938903.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(20298..21167)
                     /locus_tag="ON498_000606"
     CDS             complement(20298..21167)
                     /locus_tag="ON498_000606"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3938904.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(21168..22547)
                     /locus_tag="ON498_000607"
     CDS             complement(21168..22547)
                     /locus_tag="ON498_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3938905.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(22555..22974)
                     /gene="fabZ"
                     /locus_tag="ON498_000608"
     CDS             complement(22555..22974)
                     /gene="fabZ"
                     /locus_tag="ON498_000608"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3938906.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(22999..23475)
                     /gene="accB"
                     /locus_tag="ON498_000609"
     CDS             complement(22999..23475)
                     /gene="accB"
                     /locus_tag="ON498_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3938907.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(23478..24713)
                     /gene="fabF"
                     /locus_tag="ON498_000610"
     CDS             complement(23478..24713)
                     /gene="fabF"
                     /locus_tag="ON498_000610"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3938908.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLMALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(24732..25469)
                     /gene="fabG"
                     /locus_tag="ON498_000611"
     CDS             complement(24732..25469)
                     /gene="fabG"
                     /locus_tag="ON498_000611"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR3938909.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(25481..26398)
                     /gene="fabD"
                     /locus_tag="ON498_000612"
     CDS             complement(25481..26398)
                     /gene="fabD"
                     /locus_tag="ON498_000612"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3938910.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(26505..26729)
                     /locus_tag="ON498_000613"
     CDS             complement(26505..26729)
                     /locus_tag="ON498_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3938911.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(26782..27744)
                     /locus_tag="ON498_000614"
     CDS             complement(26782..27744)
                     /locus_tag="ON498_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR3938912.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(27754..28194)
                     /locus_tag="ON498_000615"
     CDS             complement(27754..28194)
                     /locus_tag="ON498_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3938913.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            28500..29465
                     /locus_tag="ON498_000616"
     CDS             28500..29465
                     /locus_tag="ON498_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3938914.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(29522..29752)
                     /locus_tag="ON498_000617"
     CDS             complement(29522..29752)
                     /locus_tag="ON498_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR3938915.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(29838..30725)
                     /locus_tag="ON498_000618"
     CDS             complement(29838..30725)
                     /locus_tag="ON498_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3938916.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(30814..32037)
                     /locus_tag="ON498_000619"
     CDS             complement(30814..32037)
                     /locus_tag="ON498_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3938917.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(32054..33100)
                     /locus_tag="ON498_000620"
     CDS             complement(32054..33100)
                     /locus_tag="ON498_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3938918.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(33264..34991)
                     /gene="ptsP"
                     /locus_tag="ON498_000621"
     CDS             complement(33264..34991)
                     /gene="ptsP"
                     /locus_tag="ON498_000621"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3938919.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(34991..35257)
                     /locus_tag="ON498_000622"
     CDS             complement(34991..35257)
                     /locus_tag="ON498_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR3938920.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            35736..37970
                     /locus_tag="ON498_000623"
     CDS             35736..37970
                     /locus_tag="ON498_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3938921.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            38118..38438
                     /locus_tag="ON498_000624"
     CDS             38118..38438
                     /locus_tag="ON498_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3938922.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10895 a         8289 c         6754 g        12546 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgatgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatga taaagtccat ataattgtgt
     2521 aaaagataaa aggccatgta acagcccttt tacggtacaa tgtttttaac cacaaaaaca
     2581 tacccaggag gacgttacat gacccaagta cattttacac tgaacaacga agaggttcaa
     2641 agtattattg aacattcggt aaaagatgat gtttctaaaa atattttaac cactgttttc
     2701 aaccaattga tggaaaatca acgaacagaa tatattcaag ccgatgacta tgaacgttca
     2761 gaaagtcgtc agagtcaaag aaatggctat tatgagcgag actttacgac tcgtgtgggt
     2821 acactcgaat taaaagtgcc tagaacacgt gatggtgaat tttcaccgac ggtgtttgag
     2881 cgttatcagc gaaatgaaaa ggcactgctc gcttcaatgc ttgagatgta tgtttcgggc
     2941 gtttcgacac gtaaagtttc aaagattgtt gaagagctat gtggaaaatc tgtatcgaaa
     3001 tcttttgttt ctagcctgac tgagcagtta gacccgatgg tcaacgaatg gcagaaccgt
     3061 tcactctcag gtacgaatta tccttatctg atgactgatg ttctctacat aaaagtccgt
     3121 gaggaccatc gagtgctttc taaaagctgc catattgcga tcgggataac agaaggtggc
     3181 gaccgtgaaa tcattggctt catgattcaa aatgaagaaa gtgatgacac atggtccatc
     3241 ttctttgaat acttaaaaga acgcggccta aaggggacag aactcatcat ttctgatgcc
     3301 cataaaggcc tagtgtctgc gattcgtaag tcatttacca acgcaagttg gcagagatgc
     3361 caggtccatt ttttaagaaa catcttcagt tccattccaa aaaagaattc aaaaccgttt
     3421 agagaagcag taaaagcgat ctttaagttt acggatattg aactcgctcg aacagctaag
     3481 aatgccttag tcggtgaata tatcgaccag cctaaatata caaaagcctg cgaaatattg
     3541 gataatggct tcgaagatgc ctttcaatac acggttatcg gaaatagtca taatcggcta
     3601 aaaagcacca accttcttga acgactgaac caggaagtcc gcagaagaga aaagattatt
     3661 cggatttttc ccaaccgaac gtctgccaat cgattaattg gagctgtcct tatggacctt
     3721 catgacgaat ggctcagttc tacaagaaaa tatattaagt ttgatcaatg agagaccggt
     3781 aaaacattgt atagtatttt acacaggatt atggacttga ctaaaattat gttttcttaa
     3841 gtcgtgaaac actttgatgg atcatttatt tgtggaaaac tacgttttgt tattctaaaa
     3901 aacttatata aaaaatagta aggtttttat ttcgaatcca taaaatattc ttggattgct
     3961 tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt tagccatata gaaataaaaa
     4021 atgatatcga tcgtagcaaa ttgggccaaa agtgaattag ttgccgcact tcgattcgtg
     4081 atttcttttg gtctagatgt ttgtacgagt acatcggcta tcttgctaag cgggttattt
     4141 ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt tcgccagctc ggctaatgcg
     4201 actacatctg cacttctacc tgagtttgaa acaagccaaa gcactttctt tttctcattg
     4261 ctgacaagtt gcgggagcaa gacgtggata tctttttcaa aaatgattgg cttaccgaca
     4321 cgagaccatt tttgcagaat atcttctact actaaagaag aggcgccaac cccatataaa
     4381 aaaacagtct ctgccgattc caaacactct acgacttttt ggatcgtttc ttcttctaga
     4441 atatctactg tttcttttat cgctacttgt gcattgttac caagtttgtt tttgatagag
     4501 taaaaggact catttgcttc tatatcacta aaaccttcat cgattccagt ctgtagttct
     4561 gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac cgattttttt ggaaaaacgt
     4621 acaactgtcg gcgcactcaa cccggaagct tctgctaatt cattagctgt cattgtaggg
     4681 accttctcaa tattaccgat aatgtagttg gccaactttt tttctgctga agaataatct
     4741 tccatcatac tttcaatacg actaattacg ctcataagtt cctccaccct tttaatgata
     4801 gtctatgtaa gtataccata tcagaccctt attcacggtc gtatactgga taaaaaacaa
     4861 aaaaacaatc cgcccaacag aatatcttat cccatcaatc ccacagattc tattgagcat
     4921 ctgtgtcttt gaggaatacc tgttatctat tgttcggatt gctttctatc tactactttt
     4981 ttacaggaaa tttacctatt agttttctct gctgtaattt ttgacttctg cttccatagc
     5041 atcaataaag atattcaaat cttctacagc tgtttcttta cttccataac gacgtacgtt
     5101 cactgttgca tcttccaatt ccttgtctcc aacaactaat tggtaaggga tcttttgtgt
     5161 ttgagaggca cgtatcttat agcccatttt ttcattacga tcatcaactt ctacacgcaa
     5221 ccccttcatt tgcaaacgtt ctttgatttc ataagcataa tcgccatgtg catcaacaga
     5281 aacagggatg attgttgctt gtataggtgc caaccatgtt ggaaaagcgc ctttgtatac
     5341 ttctgttaaa taagcgacaa aacgttccat tgtagaaaca attccgcggt ggataactac
     5401 tggacgatgc gtattttccc cgtcttctcc aacatatgtc aaatcaaagc gctctggcaa
     5461 taagaagtct aattgtatag ttgataatgt ttcttctgta ccaagagctg ttttgacttg
     5521 aacatctagt tttggtccgt aaaaggctgc ctcgccttct gcttcaaagt aatctagttc
     5581 taattcatcc atagcggctt tcagcattgc ttgggctttt tcccacattg cgtcatcatc
     5641 aaaatattta tctgtattgt ttggatcgcg atagctcaag cggaaacgat aatctgtaat
     5701 attgaagtca gcatatactg ccaccatcaa ttcaagcgta cgtttaaatt catctttgat
     5761 ttgatcagga cgaacaaatg tatgaccatc attcaacgtc atttctcgta cacgttgtaa
     5821 acctgacaaa gcacctgatt tttcatagcg atgcatcatt cctaattcag cgatacgaat
     5881 tggtaattca cgataagaat ggatcgtgtt tttgtagacc atcatgtgat gagggcagtt
     5941 cattgggcga agaacaagca tttcgccatc tcccatatcc attggcggga acatgtcttc
     6001 ttgataatga tcccagtgtc cagatgtttt gtacaactca acatctccca ttattggtgt
     6061 atatacatgt tggtaaccta gactaacttc tttgtctacg atataacgtt cgatcgtgcg
     6121 tcggattgtt gccccttttg gaagccagaa tggcaaacca gaacctactt ctggggaaac
     6181 catgaacaag tcaagttctt ttcctagttt acgatggtct ctttcttttg cttcttcacg
     6241 gagacggata tattctttca aagctttctt gtcaaaaaag gctgtcccgt atacacgttg
     6301 catcatttga ttgtttgagt ttccacgcca ataagcacct gccactgata ataatttaaa
     6361 tacttgtata cggccagtgg atggaacatg tggtccacga cacaaatcga tgaattcgcc
     6421 ttgttgataa gcagtaatca cttcttcttc tggcaattca ctaatcagtt ctactttgta
     6481 cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa agaacacggc gttcgatcgg
     6541 cagattttct ttgacgattt tcatcatttc tgcttcgatt gccggcaaat cttctgcagt
     6601 tacttggttt tgtccattat ctgtatcgta gtaaaaccct gaatcgatag ctggtccaac
     6661 gccaaagtgg atatttggat agaggcgacg catggcttgg gccatcaaat gagctgctga
     6721 atgacggact aagccaagtg catcttcatg gtctggtgtg atgatctcaa tacttgcatc
     6781 ttcctcgatt ggacgagtca aatcaacgag ttttccgttc actttaccag ctaaagcttt
     6841 ttttgctaag ctttttgaaa tgctttcagc aattgccaat gttgttgttc cagactcaaa
     6901 ttctttgact gcaccatctg gaaaagtaat ttttatcata atagtttcct ccttgaaatg
     6961 ttttgattga tcagatcatc aagctgaaaa acaaaaaaat cctagcatct cttgaaaaga
     7021 gatactagga cgaaaagttc gtggttccac ctaatgttca aacgcaaatg cgttcctcaa
     7081 gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt tcgaaagtgg tcatcctttg
     7141 gttttctcct ggaaaagttt cagcctagct tttcctctct tatcgaagac tctcaaattc
     7201 agttggcttt ctcatcaatc aattatgtgt ttatttaacc actaataaat agaaaaatca
     7261 agtgggaaac taaacttatt ttttccattc cccatttaca ccgtatgttc cttttttcat
     7321 ctcattgatg atgacatgga tgtgttcttt tggtgcctgc gtgtttttat gaacagcttc
     7381 cgtgatatct ttcatcatat tttctaattg ttccggactg cgaccttcta ctaattcgac
     7441 atgtacgaat ggcataatgc tctctcctct ttctgatcgt tcttttttca actcgattct
     7501 atcatactat gtttgtcttg ggtactgaat caggaaaaat ggctggatct ttttttgctc
     7561 tcaaggcact caaaatagag accgtatcca cgacttcttg caaaacagct ccgaataatg
     7621 cggggattag tcccgtactt gcaattaaca tcaaaaaaat acaaataaag atgccaatca
     7681 gtacagactg tttcgctatt ctcatcgtat ctcttgaaat cactactgct tttgctacac
     7741 gcaagagatc atctttcaag atgaccgcat ctgcactttc actagcagct gtagaaccat
     7801 gagcccccat tgcaataccg atatctgcag cagcaagtgc tggtgcgtca ttcacgccgt
     7861 ctcctaccat cgcaacaggt ctttgatctt tagggatact ttctaaaatg gtcagtttat
     7921 cttgaggcaa gctacgggca tgtacagaat ctaattggac tttttctgca atcgtttgag
     7981 caatggcaga tccatccccg gtgatcatca tcaaattttt cacatcaagt tttcttagct
     8041 ccttgattgt ctttgctgct tctgggcgaa tctgatccga aaataggatc gttcctgcaa
     8101 aagatcgatc aattgttaca tagattgctg tattggacgt ttctatttct tcagctgaaa
     8161 caaaagaggc acgtccaatc tttacttcat gattattgac tactgcttgg attccttgac
     8221 cggtcacttc tttcagttga ctaacaggat acagttggct gtttttcacg ttgttcacta
     8281 acgaacgagc taagatatgg ctagattcct gttctgcact agctgcatac agaagtagct
     8341 cttctttttt tattccattg actggctgaa tcgcctctac ttccaatatc cctttggtaa
     8401 gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc gagcttttca atcgttgttc
     8461 cagtttttac aacgatcccg tttttacttg aacggctcat tcctgcaact aaagcaatgg
     8521 gcgcagctag aatcagtgga caaggtgatg ccactaccag tacctcagca aatcgtaaag
     8581 ggtcttttga gaaccaccaa gaaataccac cgatcaaata agcaatcaac gtgaaaggga
     8641 ctgcataacg atcagctaaa cggacaaatc gggcaggctt ttcttttgat tcttcgacta
     8701 gtttaataat catttgatac tggctgtctg cggcacgttt ttcagcgatc atttggatcg
     8761 atccgtctcc atttaaagac cctgacataa tctgatctcc aggtttcttg gtttccgggc
     8821 gggactcacc tgtcaaagaa gattcatcga ccgaagattc tcctttggtc actcgaccat
     8881 ctgcgggtac aagttcgcca ggcttgacta gtaactggtc atcaatattt atttcatcga
     8941 ttactatatc ctgcaatttt ccttctttga atcgatgggc aattcttggg gtgttatcta
     9001 acaaagactg cagttcttta cccgctcgtt tattcgcgta atcctctaga ctatctccgc
     9061 ccgtaagcat gatcaatatc atcaaactag cccagtattc tttgacggct aatgttgcaa
     9121 ggattgcggt aatcgctaat acatctacgc catatcttcc tgaacgtaac gtcttgatca
     9181 tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa tgcgacaatc gcaaatgcaa
     9241 gggccggctg gtgaaaaaca aattcactta ataaagctat cccaccaata agcaaagtaa
     9301 gtaaaaattt tttgaaatgt gtcattgttt tttctcctct tttatgtgac ttagagaggt
     9361 tagtaataaa tttccagctg cctctttcat cttaagcata gtccttgact agcacttttt
     9421 aaaacaatag gccttaattg aaaacaacta tcactatcag cgtttccaaa attcatcagt
     9481 tctacaaaca aaaatattca gcaaggatta ctttttcgat tcatcagtta aaaagtacct
     9541 tttttcgaaa aactatgcta tacttttaat tcgttattta cggcggaagg agcaaacatg
     9601 cgatgaatga gaaacaacta gaacaaacgc acgtggatga aacgatcgaa ctaatccaga
     9661 tcgaacaaaa tatgctggat acaaaacaac aagaattgac tcaggaaatg aatcattcga
     9721 caaaagacac agctaatcac aagattcgag ctggttccaa tgaagctttt tatgaatctg
     9781 ctgttgaata tcgccaacat gagcaggaat tgcttttgaa ataccatact cttgaagcac
     9841 agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag tccttatttt gcccgtatcg
     9901 actttaaaga agaccgtgaa acagaaacat tatatctagg tatcgcttct ttaagagatc
     9961 tggcagaaga aacaatcgtt attgactggc gcgcaccaat tgccaatctt tattatgaag
    10021 gtgagattgg tcccgcattt tatgaaactg aaacagatag aatagatgtc gatctgcagt
    10081 tgaaacgaca atttaaaatc caagaaggac gtattttatc tatggtagat acgtcggaag
    10141 taatcaatga tgactttttg ctagagatat tggatgaatc atcaagcaac catatgaaaa
    10201 acatcgtctc tacgatacaa aaagcacaaa atgcaatcat tcgggacaca acaagtaaag
    10261 taatgctgat tgaaggaatc gccggaagtg gaaagacatc cgctttgctt caacgtattg
    10321 cttttctgtt gtatcataat cgcaagtggc tcgaggctga aaacgttctt ctattttcac
    10381 ccaaccattt gttttctgat tacatctcga ctgtattgcc ttcattaggt gaaagcggcg
    10441 tgcctacaca gacattcaaa aattatttgg tgcagctgct gccagagttt tctttactag
    10501 atgaacagca acaagaaagt ggttttttgc taggggaaaa agatccgata caagtgatga
    10561 aaagcggatt gaccttagtc gatcagattg atcgctatat ccaaggcatc acaaactacg
    10621 gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat cctatcaaag gaatcgattc
    10681 gccaatggta taaagaaaca aatgaacaat tacctttaca tcatagactt tctctattac
    10741 aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg
    10801 tcaaagatct ggcggaagaa cagctccaag aactatacgc aacagatcca aatcttgaat
    10861 atacagaaca aaaggagcgt tcattacgta aaaaactggc caaccaaatc gtacgtaaaa
    10921 gattccgtcg aatccatcga gggatcatga attatcaatt tgtcaatata acgaaacaat
    10981 atctccactt tctacaaact gtgccacttt ctcttctaac acgacataaa atcagcgaga
    11041 ccaggtggca ggaacatttg acaactgttc gtacacactt gcgtcaaaga gaattgcgtc
    11101 aagaggatgc tgtactattc tttttactga tgcgccgaac atatccagtg acggttacac
    11161 acaaagctcg ctatatcttt attgatgaaa tgcaggattt cgcaccatca caagtggcat
    11221 tgctgcaagc cttgttccca aaagcaaatt tgactttctg tggagatcta aatcaaaatg
    11281 tctttggaaa tgaaacgatc gttggatcat tggaagacct tttcgtggac cgtgaagtca
    11341 ctcgtttcca attaacgaca agctatcgct ccacaaaaga aatcactgat tttgctaatc
    11401 attttattgt gaaagaacat caagtagaaa caacagcaag aaaaggagac cttcctttgc
    11461 tcatccaagg aacaacagat gatatcaaat tgaattggtt agcagatacc attcgaacga
    11521 ctcagggaaa agcccggtat tggcgtacag caatcatagc taaaacacaa gaagaatgcg
    11581 aagctcttta tgctcaactg ccagacgagt tgaaacaaca agtccagctg attttaaatg
    11641 agagtgactt tatgaagcgt tccattattt tacttccagc ctttttagca aaaggtctgg
    11701 agtttgaccg agttttcttg tgggatgtag atgcagaaca tttttcttct cagcaagaca
    11761 aattgatcct ttacaccatg tgtacacgtg cgatgcatga attgaccctt tttacctcca
    11821 ataatgaaag tcctttgtta aacaatatgg atatttcatc ttatgagaaa aaagtactgt
    11881 aagtaaaaag acaactgcta tcattaatgt tagcagttgt ctttttattt acaataataa
    11941 ttgcagaaaa agtaattcaa tcatttaagg tcatttattc tatatataaa tccaaatctg
    12001 tttcaggata ttttaatttg acaagcgaaa taatcatttt cagttcttcc atgttttctg
    12061 gatattctgg gtcaaatacc ttgctatgac ggccaatccc gccattgtcg atcaatcggt
    12121 aaattcgatt ccattcaatg aattggtcaa ttgattggaa attgctgatt ggatagccgc
    12181 cacaatccaa aatctctgat acacgaaatg ctaggtatgc actatcgatc actttatttt
    12241 ttttcgtgtc tatcttctca tttctttcga taagaatttt ttcaggacga tgtttcaagc
    12301 tccactctaa tagtagaaag aaagcctcca tccgatactc ttcttcaaat aaccgaaagg
    12361 ctgaatcaga acgacgaaaa agcagcggaa tcagataatt ttgtctataa atcagcattg
    12421 gtgcgcggtc caccatacac tctaaataac ggttcagata ccctgtaccg acatagtttc
    12481 ctaatcgata gccttcaacg ccgaatcttg cgtatttttg cgctaatttt gaacaatcag
    12541 ttacgttcac agctaactct tctactttgc ggtaaccgct taagatacgt tcaaaaggtt
    12601 ctaaagtgat ttttgcttcc atattcttcc ccgtccatct tccacatact tcatatcatt
    12661 tcacatgata cttatgctct catcatacca caggttgaat tagaaaagaa aataaatcac
    12721 acaaaaaagc aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac
    12781 ttttcgttaa aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca
    12841 tcttctagat cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat
    12901 agtgttacct cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct
    12961 ttttctttta atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt
    13021 tgtccttctt cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg
    13081 atctcgtcag cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa
    13141 accgctccgg aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct
    13201 gctgttcgat tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca
    13261 aatccttcat agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt
    13321 gctcggtcaa taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt
    13381 agtttttggt ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc
    13441 ccaaattttg cataaatccg gctattatta gcatctttag cagttttctt tgctacgata
    13501 tttgcccatt tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca
    13561 aaaacgtcct gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt
    13621 ccttcattct tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt
    13681 ctgttccgga atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc
    13741 ttcttgtgcc tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa
    13801 gaatcttctt ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac
    13861 aggaatctct tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt
    13921 ccatgtatat ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc
    13981 agctgctgtt ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc
    14041 cagcaaagta gaagataaca accgataacg caaatacggc caataagatt cccaagtctg
    14101 cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt
    14161 tccttcttct aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat
    14221 tttttcatga tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata
    14281 cttggcattt tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag
    14341 cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt
    14401 ttgatcagac agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg
    14461 tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca
    14521 gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc
    14581 ccattcgacg ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg
    14641 atgccattta tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc
    14701 aatgacgacc tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt
    14761 gtgaaaagga ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag
    14821 ttatcgataa aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg
    14881 aactatataa actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga
    14941 gcaatgccta tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa
    15001 ctcgagcagg caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc
    15061 tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc
    15121 cagcattctt aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg
    15181 aataaatcga ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct
    15241 gtgtttctcg cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg
    15301 atagctatat tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa
    15361 tcggccattt tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct
    15421 atttttcata tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc
    15481 tgataaatcg agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg
    15541 ctggctggca atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc
    15601 ttttgcaaag gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt
    15661 gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat
    15721 cacttcatcg taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact
    15781 aagatcgact agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg
    15841 cgcatggaaa acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc
    15901 ttttccttca atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc
    15961 tgtaattgcg ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa
    16021 caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc
    16081 ttcaatcgca atttttaatt gttctttact atctatgatg atttcctcct aatatgagca
    16141 gcttattttt ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt
    16201 tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga
    16261 tcagcccatg caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata
    16321 tagtctgcta cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc
    16381 acccggattg catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa
    16441 gcgccaacaa gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg
    16501 tctcttacga ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc
    16561 aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct
    16621 gtttcttcta ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt
    16681 tgcatcaggt tttctctaga ataagcagta tattcattac tatagtccat attcatacaa
    16741 cccttcattt tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc
    16801 ttctttgatc ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca
    16861 aattacagga tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac
    16921 catagaagtc agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc
    16981 gataataaag taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata
    17041 ggtgtaactt ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc
    17101 ttccaccgct aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc
    17161 aacatgaggt ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc
    17221 agcttgttca gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat
    17281 ccctacttgc tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg
    17341 aacaaatgtc ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt
    17401 tgatagcctt tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat
    17461 gatttggacg atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat
    17521 ctcatctgta ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc
    17581 aatttgaacg atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag
    17641 atgatgcgct ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct
    17701 tcttccttaa atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc
    17761 aattactact ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt
    17821 ttctttcaca gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg
    17881 aggaggctgt aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt
    17941 cgttcgatga atatttacat catttgacac ataagcaata aatgaagcac gattgccgct
    18001 gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata
    18061 aactttaggt ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta
    18121 gtgcgccatt tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc
    18181 taaaggaatc tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc
    18241 ttgtactccc gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt
    18301 tactccgacg atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc
    18361 cgtcatctca tgagatttct tagcactagt taataataca tctactggac gaattcctgc
    18421 ttcgtaagca cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa
    18481 tatatccact ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc
    18541 tgggattagt gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt
    18601 ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt
    18661 ctcattataa tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc
    18721 tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac
    18781 ggcaccacag ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata
    18841 tcagggtgtt ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt
    18901 gtcttccaac tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga
    18961 ttggctccac gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct
    19021 aaatccaatt gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca
    19081 acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa
    19141 acaacaacaa cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct
    19201 gttccatctg gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg
    19261 atgcctagat tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt
    19321 ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag
    19381 tactcctcaa ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc
    19441 gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa
    19501 atttagaaac aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt
    19561 tatgctgatc taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt
    19621 gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa
    19681 cgattcctaa ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg
    19741 cgcgactacc gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa
    19801 tcgtttcttc cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc
    19861 caccttcacc aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat
    19921 ttctggcaat agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg
    19981 ctgtattgat caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta
    20041 aagcttttcg atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca
    20101 gatcccgtcc tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc
    20161 caccaacaat cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat
    20221 cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa
    20281 cgacatcatg tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga
    20341 agcgtatgga ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga
    20401 gataataaga attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg
    20461 atgacgcgtc ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct
    20521 tccattgcaa aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa
    20581 aaaagacctt cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga
    20641 gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga
    20701 ctttcctttg ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata
    20761 cttcccatga taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt
    20821 gctttaccag tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt
    20881 tccgtatatt cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca
    20941 aaactttttt catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat
    21001 ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa
    21061 tttggacact ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct
    21121 ctattaggat tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct
    21181 atctccgttt ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa
    21241 tcacctgcta tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg
    21301 attccttctg tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc
    21361 gtatttccat ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat
    21421 cccgggtaaa ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt
    21481 tggatcttcc ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat
    21541 tcgattgcgt gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct
    21601 gctatctcca cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt
    21661 tccacttgga ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga
    21721 aactcgatcg ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct
    21781 cctagtgctt ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt
    21841 tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga
    21901 tcacctaaga tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata
    21961 tcatcatttc caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc
    22021 tcttcttttg attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc
    22081 atcactggat aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca
    22141 ccagtacttc ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc
    22201 ttatttccca ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct
    22261 tcacacatgg aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct
    22321 tcggcttttg tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg
    22381 gcttttgccg ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca
    22441 tctgcttctg aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata
    22501 cgtacagcga tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc
    22561 aatcataaag gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt
    22621 gcctattccg gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc
    22681 cggtgtaact ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc
    22741 cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat
    22801 caatacccct ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat
    22861 cgttacattt ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat
    22921 caaaagcatt ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt
    22981 ccctctttcc tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac
    23041 aactgactcg ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc
    23101 attcatcagt ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc
    23161 tacttttttg aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg
    23221 cgaaacaacc tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc
    23281 agttgttgct tgttcaggta tgacattgac tggctccgaa actggcaacg tttcgtttgt
    23341 ttgaaccata ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc
    23401 ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat
    23461 ttcattgata tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg
    23521 ccaaacccta atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg
    23581 acaactgtca agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat
    23641 tgatgttcta atgccatcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa
    23701 tgtccagtca tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg
    23761 atttggattg cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg
    23821 atatatccga cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta
    23881 gatgcaccag aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg
    23941 tatccaacga tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca
    24001 agaactagaa cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga
    24061 atcgaaccac gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca
    24121 atccccatct cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca
    24181 tgtttgatgt tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg
    24241 atagctgtac aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg
    24301 gcaatcgcca ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga
    24361 ataacttggt tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac
    24421 cggtcttgat tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg
    24481 tagattccat agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta
    24541 tcgtaaggga aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat
    24601 ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg
    24661 tcatttccaa taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg
    24721 ctctcctttt cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg
    24781 atatatggat tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa
    24841 cgctgcatcg gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc
    24901 atatcagttg tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct
    24961 tctcgtgcca cagattttgt taatcctact acgccagctt tactagctgc ataatttgct
    25021 tgaccaatat ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc
    25081 tgcttcatca tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata
    25141 tccagacatt ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt
    25201 ccggcattgt ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt
    25261 tgtcccgctt tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat
    25321 gcttcgattt cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt
    25381 gctccagctt ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg
    25441 ataaatacat tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt
    25501 catcaaatgt ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca
    25561 taaatccgct taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt
    25621 ttaacttatg gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc
    25681 ccgcgattgt ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag
    25741 gttcagagaa ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa
    25801 tagctgtatg aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt
    25861 ttaatagtaa aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag
    25921 gtgtattata atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct
    25981 cgatcgtttc aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggcag
    26041 cttctgtcat ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg
    26101 cattactagc tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga
    26161 tgattccatg ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct
    26221 gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt
    26281 agccgagaat ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat
    26341 cttttcccat accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt
    26401 ttttctccaa tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat
    26461 tcctatttca ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt
    26521 tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg
    26581 tcaaattcat cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag
    26641 tcttcttgga tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg
    26701 atgatttctt gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt
    26761 ttatttttat aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc
    26821 gccatagcct gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac
    26881 tgcttcatcc aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt
    26941 ggtcaggaac ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag
    27001 gtttgcctga tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt
    27061 gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata
    27121 cgaaccttct gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa
    27181 tgagctactt ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt
    27241 cgcttctaag agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga
    27301 ccaatcgatt agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca
    27361 gcggatcatt ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat
    27421 atcaaaagcg atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg
    27481 tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag
    27541 atttttctgt tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt
    27601 tgttacacat cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc
    27661 catcatcttg gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc
    27721 agtaatcatt ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg
    27781 caagtttttc agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat
    27841 gttcttgacc atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt
    27901 cgttagtccc aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc
    27961 tttttttact aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt
    28021 ggctacttct gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc
    28081 tgtgattgac agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat
    28141 atcattgaag acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca
    28201 ccgtccacta attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt
    28261 ttcaaaatgc aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac
    28321 tcatttaaaa caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat
    28381 ctcacatatt ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac
    28441 ttgtccatta tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta
    28501 tgttaaaaga aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc
    28561 gagatggtaa ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct
    28621 attttcatca gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag
    28681 attttgattt cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga
    28741 tccaacatta ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg
    28801 tacatattac caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc
    28861 cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa
    28921 tgccttggtt gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg
    28981 aaatgacttt tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa
    29041 agctactgac ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa
    29101 aagccggtat gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg
    29161 aacaaaagca gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg
    29221 agagtcagaa aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct
    29281 ctggtgacgt ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac
    29341 gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat
    29401 tagaaaaaga acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa
    29461 attagttgct tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa
    29521 tttattggat taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt
    29581 gctgatgagt atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg
    29641 tacacttctc tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact
    29701 cgaccatagc ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc
    29761 ctccttttct tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct
    29821 ctaaaaaaac tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt
    29881 ttgatcaaag cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt
    29941 ttcaatgctt gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca
    30001 atctttaaat ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa
    30061 atacgaggag aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca
    30121 atcactttct ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct
    30181 gtcatcgtac ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca
    30241 gaaccatgca atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa
    30301 acggcttctt ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca
    30361 ccggataccg gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt
    30421 tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca
    30481 gaaaaaatcc ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt
    30541 gtaaagagaa tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct
    30601 tctgttacta aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct
    30661 gcttctagta gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt
    30721 ttcatttttt cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga
    30781 aacaaggatt ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat
    30841 tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac
    30901 aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata
    30961 aagtttagct tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag
    31021 tgctggtgca aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa
    31081 tcggttcaaa taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt
    31141 ataagtaagc ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata
    31201 ataaagtgct acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg
    31261 cagttctgag atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa
    31321 tctggcgtta ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt
    31381 tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag
    31441 tgactccctt aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat
    31501 gtcgatacca gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc
    31561 aataacacgt tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa
    31621 gtattttaca tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc
    31681 atacatcgta tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc
    31741 aagtatccct gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc
    31801 aaccaaataa gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa
    31861 tggtacactt ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt
    31921 tgtaaaaata tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga
    31981 tgtagcaact ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt
    32041 tttcgcctcc aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt
    32101 cattcagtac actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct
    32161 cttttgctgt ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt
    32221 ggatcagtcg gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta
    32281 accagccatt atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt
    32341 ctaaaacaac gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg
    32401 aatacgcacc ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat
    32461 gatcttcttt tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa
    32521 accaaataaa tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt
    32581 tacgttcgaa aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat
    32641 attctctgaa cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat
    32701 tggaaatagc ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag
    32761 gtgttggcgt gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga
    32821 taggagaaag ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg
    32881 catgatagac aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga
    32941 ttccgtatgt attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga
    33001 taccgacttc cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg
    33061 caagcatctt ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta
    33121 tatagtataa catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct
    33181 tactgacaaa aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc
    33241 cacaccagct tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct
    33301 tccatcgtat cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc
    33361 aagcgtttca ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct
    33421 agacccatac ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata
    33481 cctgcccatt ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt
    33541 gatgggttat atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt
    33601 gtgtattgaa tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg
    33661 tctgctaaga ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat
    33721 acttccttgc cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg
    33781 cggaattctt tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat
    33841 gcacgaagca atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg
    33901 atacgcaatg cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt
    33961 agttctttat ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct
    34021 tctaatacag ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc
    34081 atatacagga attcagtacg gtacagacca actgcttctc caccattatt atggacacca
    34141 actagatctt ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg
    34201 acagtttctg cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct
    34261 gctttttcaa attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc
    34321 ccgttgacag ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg
    34381 attgctggaa tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg
    34441 tctgttacaa acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag
    34501 tcatgtgcaa caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct
    34561 aataagtgcg ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata
    34621 taagcattat cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc
    34681 gctgattcag catttacgct gttgtcttta atattttgct tgatttgacc taccatttct
    34741 ggatctgaaa gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt
    34801 tgcgctgctt tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc
    34861 aaacgagctt cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt
    34921 tgaactagca ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc
    34981 atttcaacca ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg
    35041 catcagcttc gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta
    35101 aagacataac gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta
    35161 catcagagtt aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc
    35221 cagtttctgc tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga
    35281 gtgttgtttt tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca
    35341 ccctttcatc tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt
    35401 tcagataaaa gtattcaaat tattccggat tctttttcat ccttttcttc acattttata
    35461 acaaacgtgt ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt
    35521 tcctttgacc ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag
    35581 tataataaga gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa
    35641 atctcttaaa aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg
    35701 atgtacccat ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga
    35761 atgaggcaac gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact
    35821 gtcagagctg ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc
    35881 aaaatgatcc atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa
    35941 tgcaacagca aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact
    36001 tcaatggtgg tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca
    36061 atatcacaga acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa
    36121 tcaaacgagt cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag
    36181 aaccaggtgt tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg
    36241 atgtaccgca aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc
    36301 aaggtactgg tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc
    36361 gtcaagcaga aaatgtgatc ctattcatag atgaagttca cgaaatagtc ggtgctggtt
    36421 ctgctggtga tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag
    36481 aattgcaaat ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg
    36541 ctttagaacg tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca
    36601 gtattttgaa aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg
    36661 aggccatcga agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg
    36721 ataaagccat cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg
    36781 ttgatcctaa aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt
    36841 ctcgagaaga agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa
    36901 aaatgaaaga acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg
    36961 aaaaaatcgt tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga
    37021 cacaactgaa aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg
    37081 tagatcgagt agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc
    37141 caatcggctc cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac
    37201 aacttgccta cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat
    37261 atatggaaaa acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg
    37321 aagaagctgg tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag
    37381 atgaagtaga aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg
    37441 gtcgattgac agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga
    37501 caagtaatgc cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag
    37561 gtgttactcg ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc
    37621 gttttgacgg gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca
    37681 cactaatgct agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc
    37741 ctacgaatgt caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc
    37801 ctcttcgacg cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc
    37861 acccttctat ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt
    37921 ctaaaccaga acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt
    37981 atagcgtgag acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa
    38041 aatactgttc attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca
    38101 taaaagaggt gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat
    38161 taaaccagct tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca
    38221 ctattgtcta cactgaagcc cctcaacata atgaaattct gctgatcaat gacaaacgta
    38281 aaatccagcc gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg
    38341 ccgtttacca cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga
    38401 ttccaaaaca tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt
    38461 aaagagcgtg ggacaaaagt aaag
//
LOCUS       DAJSVD010000014        38026 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSVD010000014 DAJSVD010000000
VERSION     DAJSVD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.014"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            44..3322
                     /locus_tag="ON498_000625"
     CDS             44..3322
                     /locus_tag="ON498_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR3938923.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3863..5770)
                     /locus_tag="ON498_000626"
     CDS             complement(3863..5770)
                     /locus_tag="ON498_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR3938924.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5900..6775
                     /locus_tag="ON498_000627"
     CDS             5900..6775
                     /locus_tag="ON498_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR3938925.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6855..7829
                     /locus_tag="ON498_000628"
     CDS             6855..7829
                     /locus_tag="ON498_000628"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3938926.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8009..9154
                     /locus_tag="ON498_000629"
     CDS             8009..9154
                     /locus_tag="ON498_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3938927.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9291..9635
                     /locus_tag="ON498_000630"
     CDS             9291..9635
                     /locus_tag="ON498_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR3938928.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9906..11192
                     /gene="efmA"
                     /locus_tag="ON498_000631"
     CDS             9906..11192
                     /gene="efmA"
                     /locus_tag="ON498_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR3938929.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11330..11929)
                     /locus_tag="ON498_000632"
     CDS             complement(11330..11929)
                     /locus_tag="ON498_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3938930.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12333..13454
                     /gene="mnmA"
                     /locus_tag="ON498_000633"
     CDS             12333..13454
                     /gene="mnmA"
                     /locus_tag="ON498_000633"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3938931.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13700..15121
                     /gene="arcD"
                     /locus_tag="ON498_000634"
     CDS             13700..15121
                     /gene="arcD"
                     /locus_tag="ON498_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR3938932.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15135..16253
                     /locus_tag="ON498_000635"
     CDS             15135..16253
                     /locus_tag="ON498_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR3938933.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16313..17605)
                     /locus_tag="ON498_000636"
     CDS             complement(16313..17605)
                     /locus_tag="ON498_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3938934.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17737..18315
                     /locus_tag="ON498_000637"
     CDS             17737..18315
                     /locus_tag="ON498_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3938935.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18444..18821
                     /locus_tag="ON498_000638"
     CDS             18444..18821
                     /locus_tag="ON498_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR3938936.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18754..20826
                     /locus_tag="ON498_000639"
     CDS             18754..20826
                     /locus_tag="ON498_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3938937.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21037..21984)
                     /locus_tag="ON498_000640"
     CDS             complement(21037..21984)
                     /locus_tag="ON498_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3938938.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22230..22694
                     /locus_tag="ON498_000641"
     CDS             22230..22694
                     /locus_tag="ON498_000641"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3938939.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22756..23799)
                     /locus_tag="ON498_000642"
     CDS             complement(22756..23799)
                     /locus_tag="ON498_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR3938940.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24176..25141)
                     /locus_tag="ON498_000643"
     CDS             complement(24176..25141)
                     /locus_tag="ON498_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR3938941.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25375..26220
                     /locus_tag="ON498_000644"
     CDS             25375..26220
                     /locus_tag="ON498_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3938942.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26411..27598
                     /locus_tag="ON498_000645"
     CDS             26411..27598
                     /locus_tag="ON498_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3938943.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27604..27960
                     /locus_tag="ON498_000646"
     CDS             27604..27960
                     /locus_tag="ON498_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR3938944.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27962..28300
                     /locus_tag="ON498_000647"
     CDS             27962..28300
                     /locus_tag="ON498_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR3938945.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28376..28632
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28695..29729
                     /locus_tag="ON498_000648"
     CDS             28695..29729
                     /locus_tag="ON498_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3938946.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29722..30408
                     /locus_tag="ON498_000649"
     CDS             29722..30408
                     /locus_tag="ON498_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3938947.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30432..31280
                     /locus_tag="ON498_000650"
     CDS             30432..31280
                     /locus_tag="ON498_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3938948.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31477..32250
                     /gene="sufC"
                     /locus_tag="ON498_000651"
     CDS             31477..32250
                     /gene="sufC"
                     /locus_tag="ON498_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3938949.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32263..33549
                     /gene="sufD"
                     /locus_tag="ON498_000652"
     CDS             32263..33549
                     /gene="sufD"
                     /locus_tag="ON498_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3938950.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33546..34781
                     /locus_tag="ON498_000653"
     CDS             33546..34781
                     /locus_tag="ON498_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3938951.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34768..35238
                     /locus_tag="ON498_000654"
     CDS             34768..35238
                     /locus_tag="ON498_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR3938952.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35243..36634
                     /gene="sufB"
                     /locus_tag="ON498_000655"
     CDS             35243..36634
                     /gene="sufB"
                     /locus_tag="ON498_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3938953.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36732..37874)
                     /locus_tag="ON498_000656"
     CDS             complement(36732..37874)
                     /locus_tag="ON498_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3938954.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(37925..>38026)
                     /locus_tag="ON498_000657"
     CDS             complement(37925..>38026)
                     /locus_tag="ON498_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3938955.1"
                     /translation="FISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT        12358 a         6997 c         8080 g        10591 t
ORIGIN      
        1 actttaagta aacgctttct aatttgttac gggaggaata cacttgttaa aaaaacgtat
       61 aaaatacagt gcgctaatga tgaatctcct tatgctcagt accccggttt taatttctgt
      121 cgttgaagtt gctgctaacg aacaacagtc tgtgaacgaa gaaaatgcac acgtgaacat
      181 tgaagggatg tcaacaatgg aggaaatacc aagaactagt gaagatatgg atacggaaga
      241 aggaaatgat cctatgatga aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga
      301 agcaaatgaa gagtggaaag gggttgtttt cggagaaagc acaagtgcta gtaggtatgc
      361 cattgaacca ttagaagacg gcgtgcggct ctcttccacg aataacggcg gaaaattcca
      421 gtcaagcgga tcagatggaa tgacctatta ttatcaagca atccccaaag acatcaactt
      481 taggatgcgg gcgacgatcg aaatagaatc ttggacctat acgaatgccc aagaaggatt
      541 tgctttaatg gtaagagacg cagtaccaaa aaatcatttg tttgggcagg cattttattc
      601 aaattcattt gccatactag gcagtcggat cgaatatgtc tgggactcgg atagacaaga
      661 agtggtcaac acgagcggca gcaaatatac gatgcgactt ggattgggga cacgggcaat
      721 cacaggaata tcatctgaag atccgcaagc tgcgccagct cctgggagtg tctcagtgga
      781 aacaacacct ttagagactt cagcagggtt cttggaaaaa gaaacaggtt cttataatat
      841 attcggaaac ggtcatggga atctgtttcc ggccacgaac tcgattacta aactggacgt
      901 agagatgaaa aaaacgaata ctggtttaga ggcatattat tacgatcctg aaacaggaga
      961 agaaatggct tctgacatca tgtatgactg ggaaaaatta tacgcttcag atgaatcggt
     1021 tatttatgca ggatttgctg cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt
     1081 tgcttactca gatcctgcga cagatcctcc tgggcaagaa agacctactc gcttgattga
     1141 tccgatttac actgtgacct ctagtaatca ttcaggaaac caagatcata ctatgtcctt
     1201 tagagcaaat gctgacggat tattgacaat taaaaataaa gcaacaggag aggtgcttaa
     1261 gaacgcaaaa aacttggcta tcactgcgaa tcgggaattt gattatcaga caaaactgat
     1321 ggaaggaacg aacgaattta ctttcagttt tacgccagat aaaaactatc cgccagaaga
     1381 atacgtagag atgactagtt atgaaacaaa agagattctt catacagtgg actaccgtaa
     1441 acctaaatat tcgacagttt atgtaacacc agaagggtca gatgcaggaa acggaagcag
     1501 acaaaatcca ttatctctga cacaagcatt gagatacgca tatgctgggc agacaatcgt
     1561 catggcgcct ggaacttatt ccttcgaaag aggattgacg attccaaaag gcgttaacgg
     1621 gacaagagaa aacccgattc agttgattgc agagaaaaat ccagaaaaca agcgtcctgt
     1681 acttgatttt aaaggaactg gaaatgggat gacactgttc agtcactact ggggtttacg
     1741 agggtttgac attacaaata gtgcagctat gcaaaaagga ttacagattt ctggaaatca
     1801 taatttgatc gaagagattc atgcttatcg gaatggaaat acaggaatcc aaatcagcgg
     1861 tagctcaaat gatcctttcg aagcatggcc cgcgcataac ttagtcaaga actgtacatc
     1921 ttttgaaaat gccgatccag gatttgaaga tgccgatgga tttgcggcca aactgacaat
     1981 aggagaagga aatgtatttg acggttgtat cgcgtatcat aatgcagatg acggttggga
     2041 cctttttgct aaaaatgaaa caggttcgat cggaaaagta atcatcaaaa actcagttgc
     2101 ttatcgaaac ggctgggtac caggaattga gggagaaggt aacggtaatg gattcaaaat
     2161 gggcggttct tctttaacgg gccctcatga attgatcaat agtttgtctt tcgaaaatct
     2221 tgcgaaagga atcgattcca atagcggaac agatattatc gtcaatagca gtacaagttt
     2281 taataacggt tcatataatg tggctcttta tacctcttca gcaggaaaca cagactacca
     2341 tgcttctggt atcttatctt tccgaacaga gaatcttgag atgagagagc agatccgccc
     2401 attgaatcaa aacgaagatc aaatctacca ctcgttgaac tactattgga atgaacgaga
     2461 gaaacaatca gaaaatactc ttggacatac catatcaaaa gattggatcg agtctctaaa
     2521 tacatgttcg aaagaaggtc agcagcctgt tacgcgctat gaaaatggaa gtatccatgt
     2581 tcatggactg atgcagatta aaccggggaa tcgtcaaaca gaaaaggaaa gagtggatgg
     2641 tcttccatta tctgtaggtg ctatttttga cggagaaacc tcaccgtctt cggaatatcc
     2701 agagtatgca atcgtacaag aaccaaatca gacaaaacca aatgaacaaa ataatccgat
     2761 tattatacaa gaagcaaatg gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa
     2821 gccttttaca gtttttgtgt caactttcac accaaatcaa ggaacgcctg tctttgaaga
     2881 tatgcgttat ttcccagaaa agatccaaat cgttgaaaca cagtcaagtg ctcaattttc
     2941 taatcatacc gctatttttt cagcaccttc aaaaggagaa tatacgctag aagtcactta
     3001 tcgttcggaa atatttgatg gggaaaaatg gtctgctcat acagaatctg ttacccagca
     3061 aaaagcgatc actgtgcgag aaactgccga tgtgcccccg ggaaatgaca aaccagaaag
     3121 agaaaatcca ggaaatgaat caccaaatgg aaaaccaaat ggaaaacctt ctcatccgga
     3181 taggacagca aaagaaagtg aaaaaaatgg cactcaagga ctcccaaaaa cgggagaatt
     3241 taataacccg ttgctagcat tagctggagg aattttgtta atcggtgtag ttgtttatgt
     3301 catgaaacag cgaaaaaaat aagtggaatt tctcctttta tgatgtaaaa caattgactg
     3361 aggaaagtaa aaatagaagg aaatgagacc aatagatgtc tcacttcctt ctatcttttt
     3421 atagatgagg ttctccttgc tgcttttgat tggctgttct tattacttga tgcaggacag
     3481 ctttttcaca atctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     3541 caaacggcaa aatatgagga actatttttc cgtttttttc tctaatactc aggtcaggac
     3601 gctgatttca caacctctcg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca
     3661 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttactac ttgaaactaa
     3721 gcagcttttt cacaacctct aatacatggt attccagaag taacttcttc gaaaaataag
     3781 ccgaaatgtc acaaaaattt gagaagcgat tttcccaaat tccttcttat ttcttgaagc
     3841 tgaccacttc tggcacaacc tcttaccaga ttcgcactct tttttctggc gctagataca
     3901 tagcgtcttg ttccgtgata ccaaatgttt catagaattc gtcaaggttt tttggttgca
     3961 cgtttgctcg gagtttagca ggggcatgga catcgatttg cagtaaaagt tgttggtatt
     4021 ctttttttgc ttttgtacgc caaatcgttg cccaattgac aaagaattcc tcgatagaaa
     4081 gatctgattc cgtttttgct gcttccaatg cacagcttag accgcctgca tcagcaatat
     4141 tttccgaaac ggttaatttt ccgtttactt ttccgtcagc aaaaggcaga ccatcgaatt
     4201 cttcgatcat tgcttgtgct ttgttttgaa attgtgtaag atcttcttct gtccaccagt
     4261 tgttcaagtt gccatattcg tcaaataaag cgccgttatt atcaaatgca tgggaaatct
     4321 catgagcgat aacagctccg attccaccat aattagcaga gcttgattgt tctagactgt
     4381 aaaatggtgc ttgaaggatc gctgccggaa agacgatcac attccgaaat ggatgataat
     4441 aagcattgac cgtgtcagca ctcatttccc attctgaacg atccactggt ttgttccatt
     4501 tgctgaaacg atcttttaat gtgagtttgc tgaaaacaag agtatttgaa agcaaagtac
     4561 caccctgatc tgcaggtact gttttgaact ggtcgaataa tgctgggatc ttatcggggt
     4621 agcctacttg gatacctaag ttatttaatt ttatgacggc tttttctttt gttgcatccc
     4681 caagccagtc attttcttct agacgttttt tataaacggc gatcattttt ttcaccattt
     4741 gttctacgtc ttttttagca gcttcgccaa aatatttttt tccgtagtaa tcgccaacta
     4801 cttgatcgaa ttgtccagag gcaaggtaat acgctgcttt tttctgaggc attgcttcgt
     4861 ctgttccaga caatacgcga gaatagattc cgctgacttg gcggaattct tctgatagat
     4921 aaccactaag agaacgtact gttttaatga tcatccagct tttgaataac ggaaaatgag
     4981 cttctgtcag gatgtcattc aaatgatcga agtacactgg atcagttacg atcactttat
     5041 ctggagttgt cccaatcaaa cccgtgacaa gttttttcaa gtccagttta tctgtgtatt
     5101 taataaatgt atcaaaagat tgcggattat acattttgct gtaatctgcg ttttcttctg
     5161 cactttttac atgaggagca atcaatttat caaattgcac tgcttgttcc gtgattgctt
     5221 ctgcctgact tttttctttg ccggcaagct ctaataactg aacagccata tcaaagaaaa
     5281 cgcctattag ttgtgcaccg ttgggatggt ctgatgcata atacgtctta tctggaagga
     5341 ataaagaagg cggataagca aagaaggcgt tcgtttgcgc attcttcata tctgcatcta
     5401 catccagatt aaatggcaat ggtaatgtat ccaacagcca ttctggaaat tgcttattca
     5461 attcgtcgaa agactgcaat gattcaactt tttcgattat aggttgaagc ggttgagcat
     5521 gcaactcatt tcttcggtca taatcgtttg ccagttgata atatgcaagg aaatgtttca
     5581 ttttttctga agacactttt tctggatggg caagcagatc gtctgtttct cccatcaaaa
     5641 gttggtcgat cccgtcaact aaatcttgga aaccgcctgt agctggttta tctgcaggga
     5701 tttttgctgt tttttgccat tcttcattga ctgcttcata gaaatcttgt ttcaatcgtt
     5761 ctttgttcat tcatcgatcg ctcctttttt gtttcgtgtg cccattatct tacaaaatga
     5821 aggaattaac aaatagaaca taaaaaattg aaaattcgaa caatcaaact gatttgtttt
     5881 tttcaatcct tcacttataa tgaaaaaaag gaggcgatcc caactgaatc aagtagtgga
     5941 taaaccttta tatttcaaag tagataaaaa aattaaaaaa ttagccagca ctcagcagct
     6001 gcagtcaaga aaaagtaaat tgcttttttt agctctggtt tttgaagatc aaagttatgt
     6061 gattatcgat caatcaggac atccggtaga atattctcca gcaaaatata cttatcaaga
     6121 gggtctttct tgtaaaaaat ggaagctgat caatgaagag ccaatcgagt tatctagatg
     6181 gatcaacaga aaagaagata ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact
     6241 cgcaaattgc tgggtgggat tgccagaaga acgttttttg aggtataaga aatgggctac
     6301 cccctcaggc tatctttgtg gtacttacgc ggcagcagtt ctccttgcct attatcaaga
     6361 ctatcgtaag gggtggatgc ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt
     6421 ggttttgacg aaggcattgc gtagccagat ccaaccacta ggacttccta cgattccttt
     6481 tcaggttagt acagggattt ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg
     6541 agcaactctt ctaggttcat ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt
     6601 gatgattggt attttgaaag tacttggcag tacatatggc aatcattggg tgacagcgta
     6661 cgcttatttt gaaactgaaa caggagaacg ttattacaaa gtacacgaca actggggcga
     6721 ttatcataaa gtcattcctg ccagttggag taacggaaca gtttcacttc cttaagaatt
     6781 tgctgaaatt aacagaaaat cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt
     6841 ttggggagga ctacatgagt aataagtatc aggatgacac gttgaagatt tttagtttga
     6901 atggcaaccg tccattagct gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa
     6961 gcgtcgtgaa gcaatttagt gatggcgaga tttccatcaa tatcgaagaa agtattcgag
     7021 gggatcatgt gtatatcgtt caatcaacga atgcacctgt caatgattac tatatggaac
     7081 tactgatcat gatcgatgca atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc
     7141 cttactatgg gtatgctagg caagaccgca cggcgaaacc tcatgaaccg atcactgcga
     7201 aattgatcgc taacttgatc gaagaagcag gggcaactcg agtactgact ttagatctgc
     7261 acacggttca agtacaagga ttctttgata ttcctgtaga taatttattt acgatgccgt
     7321 tgtttgctca ttattaccgt caattaggtc tgacgggaga tgacatcgta gttgtttcgc
     7381 caaaaaacag cggtgttcag cgtgcacgta gtctttcaga atatttgaac agcacattag
     7441 ccatcgtaga tcatgcagat gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc
     7501 aaggaaagac atgtatcatg gtagatgata tcttgaacac aggagcaacg cttgcacgag
     7561 cagcaaatgt attaaaagaa aacggcgcaa aagaagtata tgcctgtgcg tcacatggat
     7621 tattgtccga tcatgcgaaa agtattttag acaatgcacc aatcaaagat atttgtatca
     7681 ctgattctgt tttcacagaa gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt
     7741 cagaattaat gggtgaagcg gtcaaacgca tccatgaaaa tacaccgatg agtccgttat
     7801 tccgtctgga agaaaaagaa ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag
     7861 aagtgagcag cttcaaggag ctgcgacaag acttttgtca cagctccttt tttctatgcc
     7921 catgaaacgt cctctagaca aaaatgagct cttctttggt ttgttcgcta ttttatgcta
     7981 gaatgtgcct agcaataatt tggaagggat gaatgaagtg aaaactattt atctggatca
     8041 tgcggcaact acccccatgc atccacaagt aattgaagcg atgacggcaa tcatgcaaga
     8101 cacgtttgga aatccttcaa gcatacatgg atttggtcgc cacgcgcatg aaaaattgga
     8161 aacagcaaga caaatcattg cagacagtct gcaagcaaaa cctcacgaga tcatctttaa
     8221 cagtggggga acagaagggg ataatacagc tatcttagaa acagcgttct cgcgtcaaaa
     8281 agaaggcaga catctaatca ctacagctat cgaacatcct gctgttctta ataccatgaa
     8341 atatttagag acaaaggggt ttgacgttac atatctgcct gtagatgaaa aagggaatct
     8401 gtcgatcgaa gaggtcaaaa aagcgttacg agaagatacg atcttggtct ctgtgatgta
     8461 cgggaacaat gaaatcggaa atttgatgcc aatcaaagaa atcggtgaac ttttagcgga
     8521 tcatccagct tatttccata cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc
     8581 agaagaatta ggaatcgatt tattaagtat ctctgctcat aaaatcaatg ggccaaaagg
     8641 cgtcggtttc ttatataaac gagacggagt aaatatccct gcattgcttt taggcgggga
     8701 gcaggaagaa aagcggcgtg caggtacaga aaatttagca gggatctggg gtatggcaaa
     8761 agcagttgaa ctgctgtcac ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt
     8821 ccagcaattg attcttgata cattagaagc aaacgaaatc ccttatcaag taaatggtga
     8881 tttaacgaat aaactccctc atgtgttgaa tcttcgctta attggcgaag caaatgattt
     8941 gctactgatg aagcttgatc ttaatggggt cgctatttct actggttcag cttgtacagc
     9001 aggaaatatc gagccttcac acgtattaga agcaatgtat ggagaagata caccgatact
     9061 aaaagaatcc atccgtatca gctttggttt gggaaatacc gaagacgaca tccagtattt
     9121 ccttgatcaa ttagttcgtg ctgtcaaaaa ataactagtg aagtcattct tatatgaaca
     9181 gatgtctgag agaacgcctt cattttttag ggaacttaac tgaaaaacct agtaaaaatc
     9241 aggataaact tttataataa agagaaacta attttatcga ggtgaaaatc atggcatttg
     9301 aaacaacagc aacggtactg ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa
     9361 gatatacatt gcgcgataat ggatttacag aaacaaacgg aggcaattat caattgatcc
     9421 gaccattaga tgccacgccg caaagcaaag aaggattcaa attaaaaatc actgtatcaa
     9481 aagatattca aacattgaaa atgtcgatca ctacagcaaa tggattgaaa gcagtcaata
     9541 ttttcaaaga tccaaaacag aaaatgagcc aagataaatt ttattttctg atggatggga
     9601 tgattagccg tgggttattc gaaaaagtcg aatgatctgt ggatataaaa agacaactag
     9661 ttttgaaacg gcctattgtt agatgactaa ctttgtaagg acgacgcaaa ttagttgtct
     9721 tttttatttt taaatttaac acatgttgtt atttttaata ttttgctgat ttattgtttc
     9781 taagtaaaga aatttaaatt gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa
     9841 aagaatcccg tataatgatt gttgtgcaaa cgctatacaa aatgaaaaag aaaagaggga
     9901 tgtttatgga aaatgaacag tcggtcgttt taacgaattg gaagagaaat tatttgttct
     9961 ttttatcggg acagtttttg tcaggaatca ctagtatggt cgtccaatat gcgatcatct
    10021 ggtatttgac aagagaaacc ggttcggcga caattttgag ttttgctact ttactaggaa
    10081 tgattcccat ggttttacta agcccatttg taggaccgct agtggatcgc tgggataaaa
    10141 aagctctttt gattgtcacg gatattattg tagcgatttt cgcgcttatt ttagcagtag
    10201 tcggaacgat ttcagaatcc ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt
    10261 cggtcgcaca aacattccaa atgccaacga tccagtcgat tatgccgaca atcgttccat
    10321 cttctcacat aacaaggacg aatggacagt taggaatggt ccaatcagcg aatttcatca
    10381 ttgcgccagc tctaggtgcg gctttgtttt cagtcgttcc tgtcaattac cttattttat
    10441 tagatgtatt gggggcagtt tttggtgtcg gcttattgat ttttgtgaag atcccaaaag
    10501 tttctcctga aatattggaa gttccgctta ctatttttaa agatgcaaaa tttgggctgc
    10561 agcaattgat ggataataaa gggttatggt acataacgat caatggtgca tttgtgatgc
    10621 tgttgttcat gccagcaatc agtctgtatc cattgatgac gctagattac tttggtggtt
    10681 ccgttggaca ggcgggagca gtggaagtag tctacgcagt cggaatgctc ttaggcggtg
    10741 cactgatcag ttttatagga acatggaaag accgaatgaa acctattatt atagcctaca
    10801 tcattatggg cctgacgatc ggtgcaagcg gattggttcc aaacgatagt caagggttct
    10861 tgtactttct tatcttgaat gcaggagcag gttgtgcaac gccatatttc aatacactgc
    10921 tgatggcaat gatccagcag agttatgaat cgaatgtttt aggtcgtgtc ttagggaact
    10981 tcaactcttt gatgaacctg gcaggtccaa ttggattatt atttgcagga ccattagctg
    11041 atcgtttagg ggtagaaaaa atgttcttgt tttccggaat cgggatttta ttatgcggaa
    11101 tcgttttatt tttaacgtca gctgcccgaa attatgataa agaattacag aaaaaactgg
    11161 taaaagaaca ccatgagcaa aaagacgaat agtatggaat ggagttgtga cagaagtctt
    11221 gttacaactc catttctgat cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa
    11281 tatgagtaat tatctttatt tgtttaatac ttgatatagg gcagtttttt cacgacctat
    11341 cgtacttcct ctttgcttca tagatttctt tgatttgttg ctcagataac ggagtgccaa
    11401 atggtagttt ttcctttaag agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt
    11461 tctcgatagg tacgctgtaa tgatggatat gtccatgaag attgcggata gtgtcgttag
    11521 gcccgagcaa catcggataa tgagtcaagt aatattgttg atggtcgaat ttaacgatgg
    11581 cacctacatc ataaaaataa aatttgggac ttccgtcagt aagcctagga tcattttttt
    11641 ctaaataacg gaaaaaagcg cgactgtcat ggttcccttt gatcaagtgg attgttcctt
    11701 gcagttgagt gaccagttcc aaaatttctg catttgcttt ttcatattgc ggatgcatag
    11761 caaggtcgcc tagatgataa acatggtcgg tttcttttat tactgcattc cagctagtga
    11821 tcaactgttg gttcatttca tccacatttg caaagagccg cggagcaaag tcgttttttc
    11881 ctaataagcg ttcatggaaa aagtgcatat cactgataaa gtaattcatg tatcttttgc
    11941 ctcccttttt tcttagagta aacaaaatca cttatctgag caactcataa catctaaaaa
    12001 gaaaaggttg taaatgagac gtgcagcttt aagcgataag aagcaatttt gaaaaatagt
    12061 ttttcacaat ttataaaatt attccttatt gccgaaaggc tggccctttt tatgagtatg
    12121 ccaatttttt atatcgaatg ctcaaaagat gagtgatttt ttggttgact atcatgtttg
    12181 tgtactgaat aggtccagta gaacagtatt tttacgactt gataattaag ggatgaaatt
    12241 ttcagaaata tgacgtataa tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg
    12301 tttcagaatc taattgaaat gatggtgata tcatgacaga caacagcaaa acacgcgttg
    12361 tcgtagggat gagcggcggt gtcgactcat ctgtaacagc acttttgtta aaggaacaag
    12421 gatacgacgt gatcggaata ttcatgaaaa attgggatga cacggacgaa aacggtgtat
    12481 gcacggccac ggaagattat aaggatgtag cgaaagtagc tgctcaaatc gggattcctt
    12541 attactctgt gaattttgaa aaagaatatt gggatcgtgt atttgaatat ttcctagccg
    12601 aataccgcgc aggacgtaca cctaacccag acgtgatgtg caacaaagaa atcaaattca
    12661 aagcattctt ggattatgca atggatcttg gcgccgatta tgtagctact ggacattatg
    12721 cgcaagtcac tcgcgatgaa aacggcgtgg tccacatgct tcgtggagta gacaataata
    12781 aagatcaaac ttacttcctt agccaattat cacaagaaca gctttcaaaa acgatgttcc
    12841 ctctaggcgg catggaaaaa tcagaagtac gggcaattgc tgaacgcgca ggccttgcga
    12901 ctgctaagaa aaaagattca acgggaatct gctttattgg tgaaaagaac ttcaaacaat
    12961 ttttgagcaa ctatctgcca gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca
    13021 aaggacaaca tgctggcttg atgtattata cgattggtca acgtcaagga ctagggatcg
    13081 gtggcggagg agattcgcaa gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca
    13141 cattatatgt ggggcaaggg ttccatcatc ctgcattgta tgccacaagc ttggatgcaa
    13201 gtgaaatcca ctttacgacg aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat
    13261 tccgttatcg tcagcaagac gttcctgtga cagttcgctt attggatgat aatcgtgcag
    13321 aagtcgtatt cgacgaacct gtacgtgcaa tcactcctgg acaagcagtt gtattttatg
    13381 atggaatgga atgtctgggt ggtggtttga tcgaccacgc atatcaagaa acaaaagttt
    13441 tacaatacgt ttaagcgata ttaaatgaat caaaacagct atggctaaca aaagaaacac
    13501 cttttctttt ggatagcata gctgtttttt tgcaataaaa ttaattttta ttcattttat
    13561 acttgaaata cttgtgtaaa aagttcataa tatccaataa tatgaataaa gttgcttttt
    13621 gttaagcaag agcagttgca aaatagaagg gaatcttttg caagtgattt gttcaatttt
    13681 ataagtgagg gtgatggaaa tggaaaagaa atcgacgggt attagcaaat ggggactggt
    13741 tgctctggtt gtcagttcgt caatcggaag cggtgtgttt gggatcacaa gtgatctggc
    13801 tagttctgca gctccagggc cagcgatcct ttcatggata atcgttggga ttggtatttt
    13861 agctttggta ttatcgctga atcatttagg agaaaagcgt ccagatctag atggtggtat
    13921 tttcggttac gcggaagctt catttggtaa attaggtggc ttcatcagtg gttggggtta
    13981 ctggttgtct gcgtggctgg gaaatgttgc ttttgcaacg atgctgatga gcgccatggg
    14041 agaatttttc ccgatattca aaggtggaca aaatgttcct tctattttat ttgcaagtat
    14101 ttttatttgg ggtctgactt tattagtaaa caatggagtt gaaggagcga gcgtcatcaa
    14161 tacaatcgtg acgatctgta aattagtgcc attattccta tttttaatat ttgtatttat
    14221 cgcatttaaa gtgaatatat tcactgctga tttttggggt aacgtatctg ataatctagt
    14281 gaatggaaca gggcagcaag gaacgatctg gttgcagatt aaaggttgtt tgatggttat
    14341 gatgtgggtc tttgtaggga tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg
    14401 ttcagaagcc cagcaagcat cgatcattgg tctgctttgt ttattgttta tctatatcct
    14461 tgcttcgatc ttgccatacg gcgtcttatc tcaagaagag ttagcaacta tttcacaacc
    14521 tgctatggca aatattttaa aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat
    14581 cggcttgatt atttcgatca ttggttcttg gttgtcttgg acgatgctcc cagcagaaac
    14641 aacgatgttg atggctcgag acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa
    14701 aaatgcccca acttattcgt tagttgttac tgcaatcttg actaatctat tcttattgac
    14761 tttcttagtt actgattatg cctatcaatt tgcctattct ttatgtacag cggcaatttt
    14821 gatttgttat ctacttgtcg ggatctatca agtccagttt tctttaaaag aaggaatcaa
    14881 aaaacaagta gtgatcggtg ggattgctgt agtttttgaa ttgatgggca tcattttagc
    14941 aggattttct tatgtccttt tatgtagtat tgcctatctt cctggttttt gcttctactg
    15001 gatcgcttgt cgtgaaacca aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct
    15061 gatttgtgtg gccgcactgc tttcaatcgg gttgcttgct gtaggctgga tcaagatata
    15121 agggggaaag aaacatgaaa atcgcaaatt tcggtgtaga agaatggcta aataaatggg
    15181 aaaagaaagc ggtatatgat attgcacaaa gctcgatcga agcattgaca ttagacgaac
    15241 tcgttggctt agatgggact agcgtaagta aatttttcga aaaacgcaaa aacgaaccat
    15301 tggactatgg ctggatcgaa ggatcagatg ttttcaaaca agaagtcgct tctctttatc
    15361 aaaccgtcga tccggaaaac atcttacaaa cgaatggagc gactggtgct aatcttttag
    15421 ctctatacgc gttagtggaa gcaggagacc atgttgtttc gatgcttcct acttatcagc
    15481 aattatatga catccccaaa tctttaggtg ccgctgttga ctttgttcat ttgaaggaag
    15541 aagaagattg gcagtttgat ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa
    15601 tcatctgttt gaatagtgca aacaatccta caggcacatt gctagatcgg aaaactttag
    15661 aaaaaatcgc agaaattgct cgtacagtag atgcatatgt attgatagat gaagtatatg
    15721 cgcctttaac ggataaaggt gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag
    15781 caaccaattc cctatccaaa acctactcca ttccaggtat tcgtattggc tggacagcta
    15841 ctggacctga gttggcagaa gtttttcgaa agtatcgaga ctacacgatg atttgcggcg
    15901 gagtcctgtc agatgatctt gctgtacatg cattgaagaa taaagaaaaa atattggcgc
    15961 gaaacaaaaa gatcattaca gagaaccttg tgattttaaa acaatgggta gccaatgaac
    16021 cgaaagttga acttgtcgct cccaattacg tctctacttc tttcattaaa ttagccatcg
    16081 aagaagatga ccagacattt tgtatcaatt tattagaaga aacaggagta ctattagtac
    16141 caggaagtgc ctttgattta ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa
    16201 ccttggaaaa gggattattc ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa
    16261 cactgcaagg acagtctgtg acaactgttc aagcagtgtt tttcatttac gcttattgtc
    16321 tatgagaagg agagtgccct gaagctatgg agactcctga agctttttct tctgcagttt
    16381 cttgctgatg gatctcttgt gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa
    16441 gagaagtcga aagaaagacg ccgcgataac caaatgcact ggaaacactg gagccgatca
    16501 taggccctac tacgttacca gttgcttgga aagattggtt ataactgaag atccgtccag
    16561 cagcttgatg aggcgaatat tttgtgatca atgcttggac agcagggagc agacaagcat
    16621 cagagatacc aatcaaaaaa cgtaaggcag ctaattgcca aacattcgtg acgaatgcca
    16681 ttgggatata aaccagaatc gcaaaaccca aaccaatcgc aagtattctt tcactcccaa
    16741 tcttgtcacc taagcgacca aatcttggag cggctatcaa agtggcaatc ccaggtatcg
    16801 aagcaatgat tccgctgatc aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt
    16861 acagactgat gattggacta atggaattgt tggatgcttg gatgatcatc gttgtgacga
    16921 acataccgat gacgacgtgt ggatatttca agtctttgaa aatctgtttg gcagaaacca
    16981 tgttttcttt tttcactggg acaaattttt catggacgaa aaacagactc aacaagaaga
    17041 cgaaaaataa gatgatccct gtaatgaaaa aggtagggcg atagccaaac gctgaagcag
    17101 agatcccgcc aagaagcggt ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc
    17161 caagaacttg accacttttt tcttttggcg tcccagttgc gacaagggca gtggcattac
    17221 tgatataacc agaaaaaatc ccttgaagca gacgcaatgc gactaattga tacacactag
    17281 tcacgcaacc catcaaagaa atgacgattg ccattcctaa tgaagcacgc aacagcatca
    17341 attttcttcc tttttggtcg gctaattttc cccaccaagg agagataagc gtagtcacca
    17401 agaaagtaga tgaaaaagtc aatccgctcc aaaagttcaa ttgtgacgta ctataattac
    17461 ctaacgtatc tatgtaaagt gacataaaag gcatgactaa actaaatccg ataccagcca
    17521 taaatgttcc aaaccataag acaaacaagt tttgttccca agtttctctt ttcctaaata
    17581 atttttcctt tattcttgca gccaattaga ctccttcttt ctgaaaattt tcatctacag
    17641 ctctttatta taaactacaa tatgtagttt atcaatgaaa gtctcttttt tctctttctt
    17701 tttcgtggta aactgaaaga gtgaaggatt tattttatga gaggaaacat ggaaatgaaa
    17761 aaaagaaatc tgtctcaaga gaaaatcatc gactgctttc gagaactagc agaagagatg
    17821 gatgttcagc aaataacctt tcagcattta gccaaacgct tggatatcaa ggctccctcg
    17881 ctgtataacc atttcaaaaa tattcgtgat gtcaaaacag cgctaacagc gaaacttctg
    17941 caggaactta atgaccagct gcgacgggct cttgtgggaa aaagtggtca ggaagcactg
    18001 cagatctatg cagaaactta tcgatccttt gctttttcaa atcaagcagt gtatgagtta
    18061 ctgatcagtg taccgcatac caatgaagct gttttattag atggaatcca tgaaacgaat
    18121 cagatcattt tgcagatatt tgaggctttt caattaacga aagaagaaaa agttcatcga
    18181 agcagggaac ttcgtagtat gatccacggt tatctttcct tacgtttttt aggatacttt
    18241 acaaaagaac caacaatcga tccagaagac agctattttt ggatgatcaa taactttatc
    18301 gctactttgc cttaaacaat cagattcatt tgtaacaatt ttaagatgtt cattaaatga
    18361 cccttttata agagaaaatt cttgcaactt tcttgaaaag tgactaagat gagtctagaa
    18421 aagctgaaag aaggagcacg agtatgtatc aagtaattac catgttcggg gacaatgagc
    18481 cttggtggtt ttttgaagac tgggaagaag atattgaaga agaagaaaca ttcgagtctt
    18541 tcgaagaagc acgacaagcg tacgaaaaac aatggaacga aatccatcaa aattatgaat
    18601 atatcaatgc aaaaagtaac tttctcagcg ccttttggaa tgacggagat gagcgctggt
    18661 gtgaagagtg tgacgatgat ttacagcaat acaagggact tgcgttgttg aaaaatcatc
    18721 agcccatcac agtagaaagc agaaaggaat tttatgaaac aactaattct agcggaaaaa
    18781 ccaagcgttg cgaaagacct aagcaaggtg cttggtgcta atcaaaagca caaaaattat
    18841 tatgaaggac ccaaggtgat cgttacttgg gcccttggtc atttattagg attgaaaatg
    18901 ccggaagatc tgaataagga atggcagagc tggcaaatgg acacgttgcc aatgatccct
    18961 aaaaaactag gaatcaaacc acttcctaaa acaggacatc aattaaaagc aatcaagcag
    19021 ctggctcagc gaaaagatgt ctcagaagca gtcattgcga cagatgccgg aagagaaggg
    19081 gaacttgttg ctcgctggat cttagagtat gtgcatttca ataaaccagt gaagcggtta
    19141 tggatctctt ctcaaacaga taaagcaatc aaaactggat tcaagcaact aagacctgcg
    19201 aaagaatatg atgcattgta tgaatctgct cttgcccggg caaaagcgga ctggctggta
    19261 ggattgaatg tgaccagagc cttaactgtg aagtacgcgg ataatttatc agcaggccgt
    19321 gtgcagacac cgacactagc attagtgaga gaccaagaaa agaaaatcga gcagttccgt
    19381 ccacaaacgt atttcacgat cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa
    19441 aaaaatcaat ttgctttaaa aacacaagaa gaagcacaaa aacttgttca attgatcagt
    19501 acaagcaaag gaactgtcac agaagtccaa gaaaaattaa aaacggaatc tgcgccattg
    19561 ccttatgatt taacagagat ccaaagagaa gccaatcaac gctatggcta ttctgcaaag
    19621 aaaacattaa gtcttgtaca aagtttgtat gagacccata agatcgttac ttatccgcgt
    19681 acagacagca aatatctgac aaatgacatg aaaagtacga tgaaagaacg attgcaagca
    19741 gtcagcgact ttgctcctga ggtcaagcag tatttgaaaa acggagccat cgtccaacag
    19801 acaaaagtat tccaagacag taaagtgacc gatcaccatg cacttttgcc aactgaaaac
    19861 agagcacgtt atgaaaaatt aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct
    19921 cgttttttag gactgtttgc tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa
    19981 tttggtaaag aacgattcat ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc
    20041 gaaactgaaa ctgagacgga aacagtcaaa tgggaaaaag gaatgagaat caatcccgat
    20101 ttcgccatca aaaaagaact atctgcaccg cctaaaccat tgtcagaagc aagcttgctt
    20161 ggtcaaatgg aaaaatacag tctgggaacc ccagctacgc gtgcagagat tatcgagaaa
    20221 ctaatcaaat ctgaactgat ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt
    20281 aagcaattat tagtattggt aaatccctct ttagtaacac cagatttgac agaaagctgg
    20341 gaaaaagacc ttgaagctat tgctgcagga aagaaacaag caggaacctt cttgaatgga
    20401 atcgaaaaag aaacaaaacg cttggtgaat gagatcaaat caagcaaaca agaatatcaa
    20461 gatttctcga ttacccaaaa gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac
    20521 acacgtgacg gcaaaatcta tgtctgcacc aattcagatt gcaactaccg cagaagaaaa
    20581 gatccaaaag tctctaatca tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa
    20641 ggtaaaaacg gtgcttattt ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat
    20701 aaaaaagagc agaagaagaa aatgaccaaa catgaagaac gacgcttgct gaaaaaatat
    20761 gcacaagcag aagaaccaga agaaagccca ttagcagcag cactaaaagc agcaatgaag
    20821 aagtaataag atatgaaaga ggttgtgata gaagtggtca gcttcaagaa ataagaagga
    20881 atttacgaaa attgcttctc aaatttttgt gaaatttcgg cttatttccg aagaagctac
    20941 ttctggaaca ccatttattc ggaaaaaagg actgtgacaa gacttttgtc acagtccttt
    21001 ttcatttaaa aaaatgttta cttcatttga cgtatattat tggtgaggct tcaattcatt
    21061 tggcttcact ttttctgttt ctcttatgat ataaacagga cgctgtttcg tttctaagaa
    21121 gattttccca atatatttcc cgatgattcc aagacataac aattggattc caccaatgaa
    21181 caggaagatc gagaccatgg aaggccatcc tgatgtagga tcaccaaaca acaatgcccg
    21241 caaaataatg aacaaaattg caataccaga accgacacaa gacaaagcgc cgataaatga
    21301 agcaatcgtt aacggtgcat ccgagaagtt cacgatacca tcaatcgagt agttgaataa
    21361 tttccagaag gaccaagatg tttcgccagc cacacgttcg cggttttcat aagaaatata
    21421 ttctgtttta aatccgaccc agctaaataa tccttttgaa aatcggttgt attctgttaa
    21481 ttctaggacg gcatccacca tttgacgagt catcaagcgg aaatcacgag cgccatcgac
    21541 catttctgta tcgctgattt tattgatcaa ctggtagaat tttttcgcaa agaaactgcg
    21601 tatcgcaggc tcgccttcac gagtcgaacg ccgggtgccg acacaatcga tatcagaagt
    21661 agtgatccgg ttgatcatct caggaagcag ttcgggcgga tcttgcagat caacatccat
    21721 cacggtcact aaatctcctg tagaagcttg caatccagcg tacagagccg cttctttccc
    21781 aaaatttctg gaaaatgaga caaagcggac ataaggataa gctgaggcta gcttcttgat
    21841 tgctgctaac gtaccgtctt ttgatccgtc gtcaataaac aaatattcga actcatgggc
    21901 acctttgaat ttttcaacct catcaaaaaa aagaggaaca gttttttctt cattaaaaca
    21961 tgggacaaca attgatactt tcatcgtttc tatcctttca atcgtatgtt tatatatggt
    22021 ttacattcaa aatttaataa accttaaata acacctttaa aatttgattt tacgtaaaag
    22081 aacaggtatt ctttttgtaa actaacgtat ttagtttacc atgtttcctc ggtttatcaa
    22141 acttttaaga acaattttta ttagttggca agtagttttg gatagagaaa aaggaggaag
    22201 ttccagtcta ttgactagga gcaaacaaaa tgaaaataca cacacctatc ggctttttat
    22261 ggttagatgc agatgaatcc ggattgactg ctgtatcttt tcagccaatt tcagaaagcg
    22321 aatcagcgaa tcagcgaatt ttaacgcgca cacaagaaca gtttgaagaa tattttgcgg
    22381 gaaaaagaaa agaatttgat gttcctttat ctataaagag aggtacgcct tttcagcaaa
    22441 atgtctggaa agctttgtcg accattcctt atggagaaat ccgaacgtat aaagaagtag
    22501 ccgttgctgt tgatagtccg aaagctgtgc gagcgattgg gcaggcaaat cgaaccaatc
    22561 cattgccaat cgttattcct tgtcatcgag tgatcgggca aaatggaaaa ctgacaggat
    22621 atatgggaaa cgcaatagat ggggttgaaa tcaaacgcca attgttgttg ttagaaggat
    22681 atctgcaaaa ttaaaaacag ttgtgattcg gacattgttc catgcgagaa tcacaactgt
    22741 tttttcatgt tcattctaaa ctttgatcag ccaaataaat agtaaagcca tcactgtttg
    22801 gagaagcccg acagctaatc cataagataa gaagcgtgta ccttctttca tgaattttgc
    22861 aaagtctaaa cgcagaccaa ttgccgctaa agcagtgatc tcaaaccagc tgctgataaa
    22921 atgtgccgat tggtcaataa ctacaggtaa agaaaacaag ctgttgacca aacaaaagat
    22981 cacaaaccct aatacatacc aaggaagcgg taattttcgt tttttctgcg tatcagttgg
    23041 cgtttcaaac agagctggtt gtttcttgtg catgaatttt tcaaacagat agacaacaac
    23101 aacgagcatc ataatacgta gaatcttaaa tagcatggcg aattcgacaa cagaattgtc
    23161 aagtaagctt gccccagcga ctacttgacc aacagattgg agtgtaccac ccaaaagtgc
    23221 acttttttca atgagatttc caccaaacaa ctgtaatcca agaaaaggga gagtcaacat
    23281 catcacagta cccagtagat tgactagtgt gatgatctgt cctttttctt cctctttcgc
    23341 atggatggaa ggggcgattg aagcaatagc agaagaacca caaacagcgt ttcctccagc
    23401 catcatcaaa gaagaagtat cgctgaattg cagtttttta ccaatcaagt aagcaccgat
    23461 gatagtgact gacatttgca gtaacacaaa aacaattcct tttacaccaa tttgagcaat
    23521 cgtttgagct gtgacagttg cacctagtaa aaaaacagag aattctaata gtttgctttc
    23581 agcaaatttt gtgccgattg ctagaacagg ctgtttaaaa aaagtatttc ctaataaaat
    23641 acccaataaa atagcaatag tcgctccacc taactgtggc agataaatag caagaagcct
    23701 gctgaaggca gcgatggcaa aagaaagcag taaaccgggt aagatttcgc ggaccgctga
    23761 atcctttaca gattttattg gatgtgacaa tgatttcatt tgatcggttc ctttcgtcaa
    23821 taaatactaa aagcatactc gaacatagta gcactaaacc agtattttga caaaagaaca
    23881 agaggtcgtg acaaaagtct tgtacgacct cttgtttcga ataaacggtg ttccagaagt
    23941 agcttctacg gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc
    24001 cttcttattt cttgaagctg accacttctt ttacgacctc ttggtaaacg gtgttcaaaa
    24061 gcttgcatct gcggtaataa gcccaaccag tacaaaaaca tgaacagctg tttttgtttg
    24121 gccgttctta ttacttgatg cagaacagct ttttcacaac ctattgaatc gattattaag
    24181 cgattttttt agaaacaaat tttccatgtt tgctatgttc tttaattaag aaacgtacgt
    24241 aatgagcaag aacgtagaag acaactacac ttgcaaacag acattccgca acttttaaga
    24301 aatccaatac gctactatag atacctaaac tgttgaagta atgaacagta gtaaatatag
    24361 ctgttgcaga gataacaagc gggaatgtaa aggctgcata gcttggataa aaacgaagct
    24421 tgaacaaacg tggaagcata gacagcacaa cgaaatacaa tacttgagat aaagcgaaca
    24481 atgctacgac aagataaaaa ttaggttcag caaagttttt aagataacct gttaggcaaa
    24541 gagaaccagg tgctgcaacg atcgtaatca gcggacgtgt cggatgagcc atatttctta
    24601 cgaaaaagac acgtttgatg atgatcggca ataagaacgc atagcagatc aatgaaatcc
    24661 aaaagaaaat ttttccaata agtgggaaga agttcccggc agttacagtg atgaccccaa
    24721 atccaacgta aatgataaac cagcttggat aaattgcttc aattgaaaga tcagctttga
    24781 taacatggtg ataattgaaa taaaagacaa ggatcacttg gaagataaca gcgcacagcc
    24841 aaatgaattt gacaattggt gcaatacttt catatgaaac aaaataagta caaataacca
    24901 tgagtgacat cgtaaaagtt ggtgaaacgg aggcgatgat tggatcatgc aacgtttgtt
    24961 tcgtgtgttc gaataagata aacaatttaa caaaaatgaa tatcataaga atcatagaga
    25021 taccgccagt tagattgcct aaagcaacat ggtgatagtc tttaaacaga tttccaaggg
    25081 aagctaaccc aaggatcaac ccgcagatcg ggatcggaat aattttcaaa aagttcttca
    25141 tatataatat tctccttttt atttaataga ttttagtacc aagatgaacg aatacattgt
    25201 aaaatgaata ggaatgtatg attacgagca agcagacagc tgattgtctt catctaagtt
    25261 actagttgga tcagcaactg ttttgttgta catttctatt atagatggtt ttttgtatat
    25321 ttaaatcaaa tagtttttat agtaattatc ataaaaagtt atggaagtgg tgccatgttt
    25381 aagttattaa cgacttttcg agcagtatat gaaacaagaa atttttccag agcagcagaa
    25441 gtactgtttt tatctcaacc agcagtctcg aatcaaatca aacagttgga gcgggaattg
    25501 aatatcgatc tttttcaacg taacggaaga aaagagatgc ttccaactaa gcaagccgac
    25561 ttattatatg aacggacact ggttttgtta gaagaatggg aagaaactca gcaaaaattg
    25621 ctcaatgaac aaaatgaaga agaagtatgt cggatcgttg cttcccacac ctttgctgtc
    25681 tatcttttgc ctcagttgat gaaacatctg atcaaaaggt ttcctaaagt gcattttgag
    25741 atcgaaatca cgaattctca tgaatcatta gaacaagtag cgaaacatga atccgatttt
    25801 ggttttatcg aaaagccatt agagacttcc ggcgtacaaa gatactcttt gatggaagac
    25861 cagctagtca tcgctgggga cccaaaaagc aaattgtggc ttgtaagaga agcaaactct
    25921 ggtgtttttc attacacgca gcgttatttt gaagagatga atatccaaga taagaagatg
    25981 atcgtgaaaa gtaacgatgt aattgttgcg ttgctgaagg aaggaatcgg tcgctcgatt
    26041 ttgtctaagc gctctgttcc agaaaatgtg ccaatgactc cgttgaattt ccatcgatat
    26101 ttttatttca tccaacgaac caatctggtt tctgatcagc tgctagagat tgccgaagag
    26161 atccttcgat acaatgaaag cacagaaact tctcacagcg aggagaatct cagctggtag
    26221 ataattgcat gtatcaaaaa aagttctatg atacactaaa gataagcagt aggcttttgt
    26281 actgcatttg ctcaagcaac tgcacaaacg gaaagttaat ttttcccaaa tgtctatgga
    26341 aagactttcc attatctaac tggaccgcta taataatcag cagtatggtt tttaagaaaa
    26401 gggatgaaac atggaaaaaa aattaaaagc ttcgaacgat aatcgtattg attatggcgt
    26461 gattttacca gtctttctgt tatgtttgat tggtctgctc tcattatatg tagcacttag
    26521 ccatgatccg aacaatccag cagtcttgaa aggcgtaggg atgcaagttg tctggtattt
    26581 agttggagcg gcagcaatcg ttgtcatcat gcatttgaat tccaaatgga tctggaaact
    26641 gactccttac ctttatggtt tggggttagt ggtaatggga ctcttgctca aattctatga
    26701 tcagaattta gctgcttcaa cagggtctaa aaactggttt cgattcggtg cgtttacttt
    26761 ccagccagca gagttgatga agattgcgta catcttgatg atggcactga tcgtaactaa
    26821 acataatacg caagtaaaag aacggacgat gaaatcggac ttttggctga ttgggaagct
    26881 cttgatcgtg acaataccgg ttttagcatt gatcatggcc caagatgact ttggaacgat
    26941 gcttgtcttt cttgcgatct ttggcgggat tttcctgatg tctggaatct cttggcgcat
    27001 catcgcacct gtagtaatct tggcagtagt cgttggagca ggaacgatct tcttggttac
    27061 gactgaaggc ggaagagact tgctttataa agttggattc aaatcttatc agtttgcacg
    27121 tattgattca tggctggacc ctttccatga tacgtcagga atgagttatc agccggccca
    27181 aggacttttg gcaattggta ccggtggctt gtttggtaaa ggattcaacg taagcaatat
    27241 ctatgtgcct gttcgtgagt ccgatatgat ttttaccgtt atcggtgaaa acttcggctt
    27301 tatcggtggt gcattcgtca ttttcttgta ttttatttta atctatcgta tgattcgggt
    27361 gtgtttcgat acgaataatg aattttacgc ctatatcgca agcggtttga tcatgatgct
    27421 gctgttccat gtctttgaga atattggagc aaatatcgga ttacttcctt taacagggat
    27481 cccattgcct ttcattagtc agggaggatc ctcgatttta gggaacatga tcggtatcgg
    27541 gttgatatta tcgatgagat accaaaatga agcacctgca agatcttcaa ggaggtaaga
    27601 aaattgtaca ccttttactg gtacccgaaa tgttcaacat gcaaaaaagc caaagcgtgg
    27661 ttagaccagc atggcgttag ttatcaaacc gttgatatga tcaaagaagt accgaaggcc
    27721 tcaacgattg agacttggct agaaacgagt caagtaccaa tccgtcgcct gttcaacaca
    27781 agcgggatga aatatcggga acttggactg aaagatcaag taggcgagtt tacggcttca
    27841 aaagcgagcc aagtcttatc gacagatggt atgctgatca agcgaccact gatcttacga
    27901 gaggaccagc ttgttgcaat tggattcaat gaaaaaattt atgaaggaga actgatataa
    27961 aatggcaggc aagcatttga agaaaaagga caacctttgg atcttattta atggaaaaga
    28021 atactgtgtt ggtttgacaa atgaagcgca agaagaactt ggagacatca cgtttgccaa
    28081 tctgccaaaa gcaggacaat cgctaaaaaa agatgaacct ctgatcgaag ttgaagcgga
    28141 aaaagcggtc aatgaatttg ccagcccgct aacgggtaca gtttcttctg ttaatgataa
    28201 aatcggtgaa gatatcaata tcttgaatga ttcagatgaa atgaatgcgt ggatcttaag
    28261 tttcaaagat gttgatccaa aagaatttga cgaattataa aatcagaaaa tcacttgaaa
    28321 aaagttgaca ctgattcttc cagttgctat aatcataagc aatcctatat gaaacgagaa
    28381 agctttgaaa agaagagtac atgtcacgga cgtttgaaga gagccctgtt tgctgaaaat
    28441 gggtaacgaa aatgagatgg aagatggtct tagagcagag taagcgagcg taagcaaact
    28501 tactacggga gtgcccgtta cagcgcgaca gtatacggaa agaagctaag tccaagtact
    28561 ggaagaggct gttattgcga agtaacggta aatcaaggtg gtaacacgga agttcaagct
    28621 ttcgtccttt tgcttaaggc aagagggcga aggctttttt gtttgaaaaa ttataagaga
    28681 aagatgtgaa tggcatgtca ttaatccaac taacgaatgt caaaaagaca ttcaatggga
    28741 aaaacggaaa catccaagcc gtcaatgacg tttcactaaa cgttgaaaaa ggggatattt
    28801 atggaatcgt cggctattct ggcgcaggaa aaagtacatt agtccgtctc ctgaatggac
    28861 ttgagcttcc cacgagcggc gaagtgatcg taaacgatca agatattaca aaattaaaaa
    28921 acaaagaatt acgtacattc cgaaagaaaa tcggtatgat cttccagcat ttcaacctgt
    28981 tatggtcaag aactgtactt gaaaatatcc agctgccgct ggaattagct ggcgtaccaa
    29041 agagcaaacg taaagaacgg gcagaagaac tgttgcgttt agtcgggtta gaaggacgcg
    29101 gacaagcata tccttcacaa ctttccggcg gacagaaaca acgcgtcggt atcgcacgag
    29161 cattggcaaa tgatcctgag atcctgcttt gtgatgaggc tactagcgca ttggacccgc
    29221 aaacaacaga agaagtatta gacttattgt tagcgatcaa taagaaattg aatttgacca
    29281 tcgtcttgat cacacatgaa atgaatgtga tccgcaaaat ctgcaacaaa gtcgctgtga
    29341 tggagttggg gaaagtcgta gaagaaggag acgtattgac tgtcttcaga caaccgaaaa
    29401 aagaagtaac caaacgcttt gtacaacaag acatcgaacc agaagctgat tcgacagaac
    29461 tattagctga actcatcaaa gaaaatccaa gcggccgttt ggcaactttg acctttagcg
    29521 aaagaaatgc caacgagcca gtcatttccc aagcgattcg acgctataac atcgatgtca
    29581 acgttgtgta tggaaagatc aaacaagtga aagaaggttc ctttggttca ttgactgtct
    29641 tgatgactgg tgaaaccaaa gaattagatc aagcagaagt gttcatcaaa gaacaaggag
    29701 taggaattga ggtgatccat cgtggataaa agcttttcag aaacatatct agattttaaa
    29761 caaatcaacc cggaaacgat gaatcaggct atccttgata cactatatat gacattaatc
    29821 tcaatggtct tagtgacgat catcggatta gttttgggat tgatccttta ctctattgga
    29881 cgcaaaaaca aaccgtatgc tcgaatcatt tatggaatcg tatcgatcat cagtaatatt
    29941 ttccggtcaa cgccatttat gatcctgatg gttttgatca ttccatttac taaagcaatc
    30001 gtaggcacta tgctaggtgc gcaagcagcc attccagctc ttgttttatc cgcagcacca
    30061 ttttatgccc gtctagtaga gattgctttt cgtgaagtca gccctggtgt actggaagca
    30121 gcagatgcaa tgggagcaag ttactgggaa atcatctgga aggtcttgat acctgaaagt
    30181 attcccgcat tgatttctgg attaacagtt acaacgatct cgatgatcgg atttactgcg
    30241 atggccggcg cgatcggcgc aggtggactt ggaggtcttg cttggcagga aggctaccag
    30301 cgtggtaatc taacagtgac attcgttgcg actttaatta ttttactaat cgtatttatc
    30361 gttcaaggaa tcggtgattt cttaactaag aaaacagaca aaagataata ggatataggg
    30421 ggatgtagga tatgaaaaag aaaatttttg gatttgcagc agcgcttttg ctgactgtag
    30481 gattagcagc atgcggtaac aacagcgaca gcaaagattc agcaaataaa gcagacgaca
    30541 cgacattgaa agtcggcgct tcaccgacac cgcacgcaga gattttggaa catgtgaaac
    30601 cattattgaa agatgaagga atcgatcttg aaatcgtcaa attcgatgac tatgtattac
    30661 cgaaccaatc tttggaagaa ggcgacattg atgccaatta cttccagcat attccttatt
    30721 tgaacaaaga aatcaaagaa aaaggatatg actttgtcaa tgccggtgct gtccacatcg
    30781 aaccaatggg cttgtactct aaaaaagtca aagatgtttc cgaattaaaa gatggtgcaa
    30841 caatcattac ttcaaattct gaatcagact ggggacgcat catcacaatt ctacaagatg
    30901 ctggcttagt caaagtcaaa gatggagtag atctggaaac agcaacattc gatgatattg
    30961 aagaaaaccc taagaacttg aagttcaacc atacgatcga cccatcactt ttagcttcta
    31021 cgtaccaaaa tgatgaaggt gacctagttg caatcaacgc aaacttcgct tacggcgcag
    31081 ggttgaatcc attagaagat gccgtattgc ttgaaaagga caactctcct tacgtgaata
    31141 ttattgcgac tcgtaaaggt gatgaagaca gtgacaaaat caaaaaactg attgaagtgc
    31201 ttcaccgcaa agaagtccaa gaatggatca cagacaaatg gggcggctct gttaaaccag
    31261 taaaggctga tgcaaaataa ggtatggaac aaaaaagatg agaaataagt ctgtaatggc
    31321 ttatttctca tcttttttat acctattcta atttagaaat caaaattgtt taataataaa
    31381 taaaagatat ttgattttag taaggaaggt cattgcgctt cattctcaat tagtctaaaa
    31441 tgagtgagaa cgaaagagaa tttgggggta taatgcatgt ctgtcttaga aataaaaaat
    31501 ctacatgtgt cgatcgagga aaaagaaatc cttaaaggcg taaacctaac aatgaaaacc
    31561 ggagaaatcc atgccatcat gggacctaac ggcacaggaa aatctacctt atctgccgct
    31621 atcatgggca atccaaacta tgaagtaaca gaaggcgaaa ttttgtttga cgggaaaaat
    31681 gttttagact tggaagtaga tgaacgtgcg cgacttggct tatttttagc tatgcaatat
    31741 ccaagtgaga taccaggtat cacgaatgcg gaattcatgc gtgcagcgat caatgcgaaa
    31801 cgtgatgaaa acgacaaaat ttctgttatg gcatttttga aaaagttaga tgaaaaaatg
    31861 gctttactaa atatgccaga agaaatggct gaacgttatc tgaacgaagg tttttctggc
    31921 ggagaaaaga aacggaatga gatcctgcaa ttgttgatgt tagaacctac atttgcaatt
    31981 ttagacgaaa tcgattcagg tctggatatc gatgctttga aagtcgttgc taaaggagta
    32041 aatgaaatgc gcggagacaa tttcggtgca ttgatcatca cccactatca acgattattg
    32101 aactatatca cacctgatgt cgttcacatc atgatggaag gccgagtagt catgacaggg
    32161 aatgctgacc tagctaaacg tctggaagca gaaggctacg ctggtatcag taaagaatta
    32221 ggaatcgact ataaagaaga agaagcgtaa gggggagcaa gaatgaaaga aaaaaactgg
    32281 ctcgattatc ttgacgcggt aaatgatttt tctttaagta aaggcgaacc tgattggatg
    32341 agaaccttcc gtcaagacgc actagcgaaa gcagatgaac tgccattgcc gcatatcgat
    32401 cgggtgaaat tccatcgctg gtcgttattt gatgtgaaag aaacacaaac catcagtgaa
    32461 acaggcacga ttccggcatt tgatgcaatg aaagacaatc ctgtgcttgt ccaacaaggt
    32521 tcatggacga tttttgaaca actgcctgtg gaattagcag aaaaaggagt catttttacc
    32581 gacttgttca ctgcaatgat cgaatatcca gaactagtcc aagaatatta tatgaaaaaa
    32641 gcagtaaaca tgaacgaaga ccaattgact gcactccatg tagcttttat gaacagcggt
    32701 attttcctgt atgtgcctaa aaatgtcgtg atcgatgaac cactcgagtc cttattcatt
    32761 caagatggcg catccgatga gcacttcttc aagcatgtgt tgatcgtggc agatgaacat
    32821 agtgaatttt cttatctgga acgtttccag acaaccaaag aacaagtagc aaaatcatca
    32881 ggtaatatca tcgtggaagt catcgctaaa gctggttcaa aaatcaaata ttcagccgta
    32941 gatcaattag gagaaaatat tacttcttat atgaatcgcc gtgggcacat tttacgagat
    33001 gcttctgtcg actgggcaat tggtgtcatg aatgacggtc atgtgatcgc tgactttgac
    33061 tcagatttag caggagaagg tgcacacgca gaagtcaaaa tcgtggcaat cagctcgggc
    33121 agacaaatcc aaggaatcga tacacgagta accaataaag cccctcatac gatcgggcat
    33181 attttgcaac acggagtgat ccgagaaaaa ggaacattga cctttaatgg tatcggccac
    33241 atcttgaaag gggcaaaagg tgcagatgcc caacaagaaa gtcgtgtctt gatgctttct
    33301 gataaagcac gtggtgatgc gaatcctatt cttttgatcg atgaaaacga agtaactgct
    33361 ggacatgctg ccagtgtagg acgagttgat ccagaagaaa tgtactatct gatgagtaga
    33421 ggattgcata aagaagaagc agagcgctta gtgattcgag gattcctcgg ttctgtctta
    33481 acagcaatcc ctgtagaaca agttcggaaa gaattagtcg aagtaatcga agggaagttg
    33541 aatggatgat cgatgctaaa aagctacgca atgattttcc tatcttattt caagacgtca
    33601 atgatgaacc tttagtttat ctggataatg cagcgacgac ccaaaagcca aaacaagtac
    33661 tccaggcatt agcgtactat tacgaacacg ataacgccaa tgttcatcgc ggtgtccata
    33721 cgttagcaga acgcgcgacc catgattatg aacaagcaag agaaaaagta cgggcgttca
    33781 tccatgcaaa agaaacagca gaagtgctgt ttactcgtgg tacgacgaca agcttgaatt
    33841 gggtagccag aaccttcggc gaccgctata tcaaagcagg agacgagata gtgctctctt
    33901 acatggaaca tcattctaat gttatcccgt ggcagcagtt ggcaaaacga acaggtgctg
    33961 tcttgaaata tatcgaacta acagaagaag gcttcctgga tatggaggat gcagcgaaaa
    34021 agattacgga caaaaccaaa atcgtctcca ttgctcatgt atccaatgtc ttaggtgtct
    34081 tcaatcctgt tcaagaatta gctgaacttg tccatgaaaa cggcggggtc atcgtagtag
    34141 atggtgctca atctgccccg cacatggcca ttgatgtcca agcactagac tgtgatttct
    34201 ttgctttcag cggacacaaa atgtgcggac caacaggtat tggtgtttta tatggtaaac
    34261 gaaaatggtt agaagaaatg gaacctgttg aatttggtgg cgaaatgatc gattttgtcg
    34321 aattatacga cagtacatgg aaagaactac cttggaaatt cgaagcaggg acaccaaata
    34381 tcgccggcgc tatcgcatta ggccatgcaa tcgattattt gcaaaaactc ggtatggaca
    34441 atatccatcg atatgaagaa gaactagccg cttatgtgtt gccaaaatta caagcaattg
    34501 atggattgac tgtttatggt ccacaagacc cgaattttcg gacaggagtc gttgcattca
    34561 atttggatgg gttacatccc catgatgtcg cgacagcttt agacatggaa ggggtagccg
    34621 tcagagcagg tcatcattgt gcgcaaccgc tattgaagta ccttgaagta tcagcgactg
    34681 ctcgcgcgag cttttatttc tacaacacaa cgcaagacgc cgaccgttta gttgaagcaa
    34741 tcatggcaac aaaggagttt ttccaacatg ggactatcta aattagacaa tttataccgt
    34801 caagtgatct tggatcattc ttcccatccg catcatcgtg gaagcttgac tgactcgaca
    34861 agcaagatcg aattgaacaa ccccacatgt ggagatgtga tcgaattgca agtcgatgta
    34921 aaagacggaa tcatcaaaaa tatcgccttt gacggcagtg gctgctccat cagtacggca
    34981 agtgcctcga tgatgacaga cgccgtcatc ggaaaatcaa tagaagaagc agaacattta
    35041 actgtagact ttctacagct tgtccaagga aaagaagtca aagatcccga ccaattaggc
    35101 gatgctgcta tgcttggtgg tgtagctaaa tttcccgctc ggatcaaatg tgcgacactt
    35161 gcctggaaag cactaggaaa agcaatggaa aacgatggta ccgcagcttt ccatcattac
    35221 cacaaggaag aggagtgaga acatgggcgt accagaatta gaagaatata aattcgggtt
    35281 tcacgatgat gtcgaaccgg ttttcagtac aggagaaggc ttgacagaag aagtgatccg
    35341 tgagatgtca agaatcaaag gcgaaccaga atggatgctt gaattccgtc tgaaatctct
    35401 agaaacattt aacaaaatgc caatgcaaac atggggcccc gacctttcag acatcgattt
    35461 cgatgccatc aaatattacc aaaaaccaag tgacaaaccc gctcgtgact gggaagacgt
    35521 accagaaaaa atcaaagata catttgaaaa gattggtata ccagaagctg aacgagctta
    35581 tctagccggt gcttccgcac aatatgaatc agaagtcgtg taccataata tgaaagatga
    35641 attcgaaaag ttaggaatcg tctttactga cactgactct gctttgaaag aataccctga
    35701 attattcaaa gagtactttt caaaacttgt accgccaacc gacaacaaat tagcagcgct
    35761 gaattcagcc gtatggtcag gtgggacctt tatttacgtg ccaaaaggcg tcaaagtcga
    35821 tgttccatta caaacgtact tccggatcaa tgcggaaaac acggggcaat tcgaacgtac
    35881 gctgatcatt gttgacgaag gagcaagcat ccactatgtc gaaggttgta cggcacctac
    35941 ttattcaagt aacagcctgc atgccgccat cgtagaaatc tttacaagaa aagatgcgta
    36001 ttgccgttac acaacgatcc aaaactggtc agacaatgtg tacaacttag taaccaaacg
    36061 agcaaaagca tacgaaggcg caaccgttga atggatcgac ggaaacctag gtgcaaaaac
    36121 aacgatgaaa tacccaagtg tttacttaga tggaaaaggt gcacgaggca ccatgctctc
    36181 catcgccttt gcaggagcaa accaaatcca agatacgggt gcaaaaatga tccacaatgc
    36241 accaaataca tctagctcta tcgtctctaa atcaatttca aaagacggcg gagaagtaaa
    36301 ctatcgagga caagtaacct ttgccaaaaa cagtgcgggt tcgatctctc atatcgaatg
    36361 tgacacgatc atcatggacg aatggtcgaa atccgatacg atcccattca atgagatcca
    36421 caacagccaa gtctctttag aacacgaagc caaagtctca aaaatctcag aagaacaact
    36481 ctactatctg atgagccgag gcctgaccga atctgaagca actgaaatga tcgtcatggg
    36541 ctttgttgaa ccattcacca aagaacttcc aatggaatat gcagtcgaac tcaatcgatt
    36601 gatcagttat gagatggaag ggtctgtagg ttaataacta tttcattgct cgaaacgttg
    36661 atattaaagc gtttcgagcg tttttttatt tgtattttaa tggttggcta cgtttttggc
    36721 tacgtttgga attattcgcc cataaatttt gcaaatcggt caccagtttt cttaacagat
    36781 tgtggtgtga catgagcata gatattcatg gtagttttaa tatctttatg acctaatcgt
    36841 tcctgtactt cttttatcga cgcaccactt tcaaacaata agctacagtg ggtatgccta
    36901 aatccatgtg gagtaatttg aggctccatc ggtgtctttt tgtaaatcca gtctaaccaa
    36961 tcgtttacta cttgggggta gtacaacttg tttgtgtttg tagtgaaaag aaactgtttt
    37021 ggtgaattcg tgttatagcc atataagatc attattttca tttgttcttg ttgccaaata
    37081 cgcagttgtt taagtgtaaa atcatcaagc tgaatcgttc ttgatgaatt tttagtttta
    37141 ggtacttgta gaactacttg attaaactcg tcttgagcga tagttttacc aattgttatt
    37201 gttttattga aatagtctat atcttcccat tgaagcgcca atgcttcgct tttacgcatt
    37261 cctgtataag ctaataaacg gaaaaaagtg tacagtttaa ctgaattatt ttctttagta
    37321 ttctgtaaaa acagttgtaa ttgatctttc gtgtaaaact tcaagggttg ttcttcttca
    37381 atttttcttg gaagcaatgt tttagacata ggattatcct tacagacttc cattgctact
    37441 ccaaatttaa atatttgagc agttgttttt ctcaagaaac catattgttt taatgttttg
    37501 tgccattcat tcacctgttt ttggcaatag ctaacactta ttttatccaa tcgtaattcc
    37561 ccaaaagcag gtaatatatg gtttttgcaa aaacgttgag caacggcaac agaagacggt
    37621 tttactcttg ttctgtagtt atcaatccat aattcataca actcttgaaa agttgtaaca
    37681 ttgattttaa actcttgttt ttctttaaag tctacaagta gtctattaag tgctgtttgc
    37741 gcctcagctt ttgatttgaa tcttctacgc tttgtacgta tatcttttcc agtcttggga
    37801 tctttcccta aataagtttc aaatccccaa tatttttcgc cattttgtaa tttataacta
    37861 aaaaatgttg ccatttgatt ctctccttac ttcattattg caggtagaga agttatttga
    37921 agaatcaatt atttttaaat ttatcgtgat tatcagttaa aaattttgcg attatactat
    37981 ttttctcata tgggaaattg tattttttac taagatctga aataaa
//
LOCUS       DAJSVD010000015        36604 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSVD010000015 DAJSVD010000000
VERSION     DAJSVD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.015"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..283)
                     /locus_tag="ON498_000658"
     CDS             complement(<1..283)
                     /locus_tag="ON498_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938956.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVL"
     gene            complement(458..2593)
                     /locus_tag="ON498_000659"
     CDS             complement(458..2593)
                     /locus_tag="ON498_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3938957.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2676..4331)
                     /locus_tag="ON498_000660"
     CDS             complement(2676..4331)
                     /locus_tag="ON498_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3938958.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4333..4647)
                     /locus_tag="ON498_000661"
     CDS             complement(4333..4647)
                     /locus_tag="ON498_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3938959.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4817..5077
                     /locus_tag="ON498_000662"
     CDS             4817..5077
                     /locus_tag="ON498_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR3938960.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5091..5405
                     /locus_tag="ON498_000663"
     CDS             5091..5405
                     /locus_tag="ON498_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR3938961.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6135..6689)
                     /locus_tag="ON498_000664"
     CDS             complement(6135..6689)
                     /locus_tag="ON498_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3938962.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6815..8744)
                     /locus_tag="ON498_000665"
                     /pseudo
     CDS             complement(6815..8744)
                     /locus_tag="ON498_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(9050..9766)
                     /locus_tag="ON498_000666"
     CDS             complement(9050..9766)
                     /locus_tag="ON498_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3938963.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9767..11590)
                     /gene="glpO"
                     /locus_tag="ON498_000667"
     CDS             complement(9767..11590)
                     /gene="glpO"
                     /locus_tag="ON498_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR3938964.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11604..13100)
                     /gene="glpK"
                     /locus_tag="ON498_000668"
     CDS             complement(11604..13100)
                     /gene="glpK"
                     /locus_tag="ON498_000668"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3938965.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13236..14696)
                     /locus_tag="ON498_000669"
     CDS             complement(13236..14696)
                     /locus_tag="ON498_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3938966.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14686..15981)
                     /locus_tag="ON498_000670"
     CDS             complement(14686..15981)
                     /locus_tag="ON498_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3938967.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16313..16990)
                     /locus_tag="ON498_000671"
     CDS             complement(16313..16990)
                     /locus_tag="ON498_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR3938968.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(17020..18111)
                     /locus_tag="ON498_000672"
     CDS             complement(17020..18111)
                     /locus_tag="ON498_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR3938969.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(18212..18646)
                     /locus_tag="ON498_000673"
     CDS             complement(18212..18646)
                     /locus_tag="ON498_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3938970.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19230..20687)
                     /gene="ascB"
                     /locus_tag="ON498_000674"
     CDS             complement(19230..20687)
                     /gene="ascB"
                     /locus_tag="ON498_000674"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3938971.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20684..22579)
                     /locus_tag="ON498_000675"
     CDS             complement(20684..22579)
                     /locus_tag="ON498_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3938972.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22637..24412)
                     /locus_tag="ON498_000676"
     CDS             complement(22637..24412)
                     /locus_tag="ON498_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3938973.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24462..25958)
                     /locus_tag="ON498_000677"
     CDS             complement(24462..25958)
                     /locus_tag="ON498_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3938974.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26152..27774)
                     /locus_tag="ON498_000678"
     CDS             complement(26152..27774)
                     /locus_tag="ON498_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3938975.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28102..29415)
                     /locus_tag="ON498_000679"
     CDS             complement(28102..29415)
                     /locus_tag="ON498_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3938976.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29660..29884)
                     /locus_tag="ON498_000680"
     CDS             complement(29660..29884)
                     /locus_tag="ON498_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938977.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29904..30326)
                     /locus_tag="ON498_000681"
     CDS             complement(29904..30326)
                     /locus_tag="ON498_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3938978.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30468..31178)
                     /locus_tag="ON498_000682"
     CDS             complement(30468..31178)
                     /locus_tag="ON498_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938979.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31196..31693)
                     /locus_tag="ON498_000683"
     CDS             complement(31196..31693)
                     /locus_tag="ON498_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3938980.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31730..32182)
                     /locus_tag="ON498_000684"
     CDS             complement(31730..32182)
                     /locus_tag="ON498_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3938981.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32317..32922)
                     /locus_tag="ON498_000685"
     CDS             complement(32317..32922)
                     /locus_tag="ON498_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3938982.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33372..33992)
                     /locus_tag="ON498_000686"
     CDS             complement(33372..33992)
                     /locus_tag="ON498_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3938983.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33994..34863)
                     /locus_tag="ON498_000687"
     CDS             complement(33994..34863)
                     /locus_tag="ON498_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR3938984.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34886..35293)
                     /locus_tag="ON498_000688"
     CDS             complement(34886..35293)
                     /locus_tag="ON498_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3938985.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35312..36601)
                     /locus_tag="ON498_000689"
     CDS             complement(35312..36601)
                     /locus_tag="ON498_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3938986.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        10405 a         7852 c         6105 g        12242 t
ORIGIN      
        1 tcaatacaaa tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga
       61 aatacagcat tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg
      121 gtctcgttaa tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa
      181 gagccactaa cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt
      241 tcaccaaaat tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt
      301 ttttgttacg acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta
      361 aatttttcct aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc
      421 tttttatcag ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga
      481 caatgctttt cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat
      541 ctccatcgtt ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac
      601 tcaaaggaat gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta
      661 gcgtttgggt ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa
      721 ccaaaatctc agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg
      781 gttcttttcc ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg
      841 ttttgaaaag ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat
      901 ggatcggcag atggtagtcg tcgaaacaac gcataatatc agcaaaagta gtggaagaat
      961 taaagattgc ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga
     1021 tcaatagacc taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa
     1081 atgctggtac atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt
     1141 catcaacagt acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc
     1201 tttcaaacaa ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc
     1261 gatatttttt ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag
     1321 aaatcaattt accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct
     1381 gcacagcagg actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct
     1441 ttttcgttgc ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt
     1501 tttcgatcgt ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat
     1561 tcggatgatc taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac
     1621 gcaaacgttc tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt
     1681 caccttctat ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg
     1741 gatttctttc acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag
     1801 gtgatagaat cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg
     1861 caatacgctg catgatagca gaggttttac cacttcctgc tacgccattt accaagatat
     1921 tttgactttt cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag
     1981 ccttcatttc attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag
     2041 ctacagacgt atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt
     2101 ctttaataac cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt
     2161 tattgtaaaa aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat
     2221 catctgtgaa atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt
     2281 ctacaacgac ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat
     2341 tttctgcgtt tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca
     2401 tcgaaaaagt atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca
     2461 tttctttcaa cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt
     2521 tttttttatc aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt
     2581 gtgtgttgac catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct
     2641 cttttaagag tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct
     2701 tcaggtcgga cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca
     2761 gactgcttca agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata
     2821 tattcttgtt ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt
     2881 ttggcttcct tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt
     2941 tttccttttg ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct
     3001 gcaatgttca ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc
     3061 ggtgcatacg tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct
     3121 gttgctaaaa tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa
     3181 atctcgccag tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa
     3241 tagcttgcat gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg
     3301 gctgttcttt cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct
     3361 gttccaatac cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta
     3421 ccttgacgat tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt
     3481 tttacaacga tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg
     3541 tctactagga tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta
     3601 tcaggacgaa caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc
     3661 agtcgcaatc gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct
     3721 actccttgat gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga
     3781 agaatatgtg gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct
     3841 gccatttcca atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct
     3901 aatgcatgca tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct
     3961 tctgttatcc ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca
     4021 tcgtactgtt caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga
     4081 aagatggctg tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat
     4141 tctggcgttt ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc
     4201 aagccgcaat ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct
     4261 tccattaatc ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata
     4321 acgattttca tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg
     4381 tacattcact acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat
     4441 ccctgattga caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact
     4501 ttgtaatgga acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct
     4561 aacatcaagt aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga
     4621 ttttattgat tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc
     4681 tcatggggta tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt
     4741 tttcactact ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa
     4801 tagaggagaa tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac
     4861 agagggacaa attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt
     4921 catcactcaa ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc
     4981 agataatctg aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa
     5041 actagaacaa gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg
     5101 ttttccatat tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga
     5161 taaaagcttc tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt
     5221 atctagactc tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg
     5281 ccaatgctat gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg
     5341 taccagcagg tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc
     5401 cttagtcagt agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc
     5461 gaagaagcta cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa
     5521 gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta
     5581 ccgcagatgc aagcttttga acaccgttta aggaagaggt tgtaaaatca gcggtttgac
     5641 ctaagtatta gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta
     5701 ataccgaagg tcagctgatt gaacaccgtt taaggaagag attgtgaaaa agtcgcttag
     5761 tttcgagtat taagaaaaat tttgtgaaaa tggatttcta catttttgca taaatttggc
     5821 ttaataccga gaaactggct tttgaatacc gtttaaggaa gaggttgtaa aatcagcggt
     5881 ttgacctgag tattagagaa acaaacggaa aaatagctgc tcatattttg ccgttttttg
     5941 agctaatacc gaaggtcagc tgattgaaca ccgtttacca agaggttgtg agcctaccat
     6001 ttaactccga atcacaagaa ataatttcaa aaacaacttc tcctgttttt ggttcggttg
     6061 gcttattatc gagaagtagc tactgggagt ctatttatca aaaaaaggaa tgcgacatag
     6121 gtcacacccc ttttttattt tctttgaaac atctttcggt actcgacagc atacaagaca
     6181 aaagattgta aaagaccgta agtgatgacg gcaaaaatga ctctcaccca cagcgttggt
     6241 aattctggga aaagcagcaa aaggatcatg ccaacataga tgacagcagt cgctactttg
     6301 ccataccact tcgctccact taatttgata tttttagata gcccgattaa tccagctaca
     6361 aacataaagc cttcttttat caagaataga ccaaacaaca gccaaagcca cggtcgatac
     6421 catgccatag acaaaatcag aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc
     6481 ttccctaagt ctgttacctg attatatctc cgagcaatga ccccgtctag ataatcagac
     6541 aaacctgaga caacaatgac cgctcctgct atataaaatg actgtctcag cacaaaataa
     6601 agataaatcg gcagcaaaaa aatacggaat atcgataaaa gattcggaat cgtttgtatt
     6661 tctttacgcc agtctgtgct taatttcatt ttatcgccct acctcctcag cactcttttc
     6721 tttttatttt agcttacacg gggaataaaa ccaatgattt cactgttttt atttttcttt
     6781 acagacactg atattatgag ctaatttgag ataatcactt gctgttttca tcgtttactc
     6841 ttctttcttc ctgtcgtttg cgcatttctt ttactagtac tttatgagct ttttttgatc
     6901 ctcgctctag gtcacgctgc cgtttacgtg ttttataatc gataaacaga ctgtctaaat
     6961 cgtaacctgt cgggtacaga tcttctgctg aacgcaccag acgaacacgc tgacgaggca
     7021 cgtggattat ttttttgtct aaatatacat caatcgtatc ttcacctata tcttcataaa
     7081 ctaacgcttc tttttggtat tcattgacaa atatccgatc accttttgca aagagctgac
     7141 gttctttttt attctccttc ctatttgtaa ctgctgtctt aggaagaaat tcttttttct
     7201 gtgtccgata ggctttttcc gccaagtaac gctctgcttg atggatcaat ttcatcgaca
     7261 tatccatttt atgagcaatc cacaaagctt ggctttctcc tgtttcttcc aacccgcaac
     7321 tgatatttag gacgaagtgc ttcccgatcg aaagccatag cagccggcac aaaatcttca
     7381 tgatctccgg cgaatttctt gatttctcca taatgggtcg tggcaatgat caatgcccct
     7441 tgctcataca tagattccat gattgcgatc gctagtgcag ccccttcatt tggctctgtt
     7501 ccactgccaa tctcatctaa aagtaccaac gtattccttt ttatcttttt caaaatagca
     7561 gcgatgtttt gcatatgacc agaaaatgta ctcaacgcat tttctaaatt ttggtggtca
     7621 ccgacatcga cgaaaatctc atcaaagaca gcaagctctg tcccttcttt tgccggaact
     7681 tgcataccga acatcgccat caaagtcaat aaaccgactg tcttcaacac gactgttttg
     7741 cctccggcat ttgctccggt gataatcaag cctcgatagt ctttaccgat ttcaacatcc
     7801 aatggtaccg catgctcagg taaaaatgga tgtctgcctt gcttgataat gatatgttcc
     7861 gatttattga ttctaggtgt gcttccattg atttctcggc taaattttgc ccgagcaaag
     7921 atgatatcta acaccgtgat cgtttcgatc aataaatcga tcacttcttc gttttctgcg
     7981 atagcaccag tcaatgccgc caggacttga tattcttcac tgatttcttc agcttttaac
     8041 aactgatagt gttcattcaa tttagaaatc acagttggtt caataaaaac cgtcgttctt
     8101 ttatttgatt catcgataat cgttccatct attttatttt tatagctggc tttgatagga
     8161 atcgtatagt attcgccttt ttgaacaatc attgcttctt ggatcatttc tttgtttttc
     8221 ggatgacgca agaatttcag taattttgac tggatttctt tttcgattag ttgtaattgt
     8281 ttgcgtactt tacgcaaatt acgtgacgcg tcgtcactga ctttattatt ttttattttc
     8341 tggtcaatca actcttctac atttatcaaa tcaggaagat gtttgctata agcaaacaac
     8401 aagggtgtct ggtattgatt cttatcaaaa aactttgtga tcatccgact gcttctaaga
     8461 aaatccgcat attcgatcaa atcggttggc tgcagaatca accctttttt gacttgttct
     8521 gtcagtgcat cgatcctcgg caaccccata aatggaacat gctgactgct ttctaagatc
     8581 aagcgtgctt ctcttgtttc ttcttgacga tccaagacag tttgtaggtt tgtcgagacc
     8641 gtcatatcgc tgattcgttt tttgctatag tttccaattg ctcttgcttg gatttccttt
     8701 ttgatccgat caaattgtaa ttgactaatc atttcgttgt ccatattctc tccttttctt
     8761 gaagagatac ctgtcccaaa ataaataagg gcctagagat aaataaaagg atagaacaca
     8821 gcttaaaaaa gtcatgtcct atccttatta gaatacaaag atattgtttt cctgacaggt
     8881 accgtttcat aagcacacca aataaacttt gtttattggc tattcgactt actgattaga
     8941 taccagctac actcacaaaa cataccccat tcttctaggt cttatttatt ttccacttaa
     9001 ctatagcaag acacattttg gttgtcaaac tttagtatgt tttatagatt tacaccatta
     9061 atgaatagac acctaccgcg ataattgctc cgatcattgg acctgcgatt ggtacccatg
     9121 aatatgccca atctgaagtt cctttattag ctattggcaa taccgcatgt gctaatcgag
     9181 gaccaaagtc ccgagcaggg ttgattgcat atcctgttgg ccctcctaat gaaagaccga
     9241 tggctaagat caatccacct acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc
     9301 caaaagctag caacccaaga actaatacaa atgtaccaat taattctgtg atcacatttg
     9361 ctggataatt ccgtatagca ggacctgttg caaacgttcc taaaatcgcg ctttcatctt
     9421 tcgttgcatt ccaatgtggc aaataagaaa gccaaacaag aatggcccct gcaaaagctc
     9481 ctaaaacttg cgcaatgatg aaaggaacga caagattcca actaattgcc cctgtcatcg
     9541 ccatagctag agagactgca ggattcaaat gagctgggcc catgaatcca gaaatataga
     9601 cagccattgt tacagcgagt ccccaaccaa atgcgatgac aatccagcca gaagcctgtg
     9661 ctttactttt gtttaaattc acagccgcac acacaccatc tccaagcaaa actaggatca
     9721 gtgtcccaat aaactccccc atgatttgag tcatatcact gttcatttat ttctccccct
     9781 ttagttcccg cagatctgat tctgcaatca atgcttctaa acgtttagtc tgttgcgcct
     9841 tctcctcttc tgtccattca aaatatccag caattgcatc gatcacaggt tgcttgatct
     9901 catcaagctg atctcgttca aacaacagat gatttgtacg acgaatgaga taatcgcctg
     9961 gggccaaaac catttcttct tctaaaccat aacgtaaccg agctgactca gcgagactca
    10021 aaccaggata aggtgccatt tcttttgcta attcaaagat acgtcgggca ttagtcccgt
    10081 aaaaatcagc gatataagta gcatcttctt cctctaaacc ggctgctact ccttcttttg
    10141 ctaattctgt cactgtttct tctaatttag ttggatcgaa gtttccacct gaaatctgat
    10201 attttttaga gtcgatttct ttcgtctcta ttccgtattc ttcttttaat aattggcgta
    10261 tcaatcgcaa agctccttct gccatcttac ggtaatcggt gattttccca ccagataaag
    10321 tcagcaaacc atctggttct ctttctaaag aactacctct ggaaatcgta gaaggtgctt
    10381 tttcatcacg gctgttttcc aaatggttca acacgtcttc tacttcagca cgagaaactt
    10441 tgttttcctt atattcactt actgtatcga caactttatt gaagctcttg tctgaaatcg
    10501 atccattatc tccaccattg taatcagaac cagaattacc aatcaacagt ggacgaagtc
    10561 ctgcccagct tgcttcgata tcattcaatg tgatatttgc ttctggatag cgatgattga
    10621 tcacatccaa tagataatct acatcttctt gtgtgacttt cggatccgta aaatctcctt
    10681 ggtaatccgt atctgtcgta ccaaagtaag tcttgttttc tcttggaata gcaaaaacca
    10741 tccgcccatc ttgctttcct gtatcgaagt atgtgggttg cggtacaggc agttttttcg
    10801 catctacaac taaatggatc cctttagttg gacgcatttt aggagagact ggacgcgtaa
    10861 aattcaagtt tcgtacttta tctacccaag gaccactcgt attgatcact aattttgctt
    10921 tgatttcgat cacttcatct gtcagcaggt cacgagcttt gacgccaacg atttgatcgc
    10981 catcatataa aaagccaacc gctttcattt tacttactag ataagcccca tcttcggcag
    11041 cctttttgat attatcaatc actaaacggg catcattgtt gcggaaatct agatagacac
    11101 ccgcgccttt tagcccttct tttttcaaaa acggttctct ttccaatact tcttctggtg
    11161 tcagcgtata attctcatat ttggttcctg tcacatttgc cagtttgtca taaaggtcca
    11221 ttgctacttt gaccgagaac atattgaagg ttgttgctcc ttcgccctca tagattggca
    11281 aaagcattgg atctggtttt gggatgtgtg gggcaatact ttgtacgact gcacgttcac
    11341 caactgtgtc agctactact tccacatcaa atgtcttcag ataacgaata ccgccatgaa
    11401 caagtttggt cgagcgagag gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg
    11461 ttttgattcc tgatgctgct gactggactg ccacaccggc accagtgatt ccgccaccga
    11521 tgatcaacag atccaattct tcttgctgca ttttttgaat gctatcttgt cttgtcttgt
    11581 ttgaaaacat atggatttcc tccttattca aatgcttggg tagcttttac tgcttttttc
    11641 caaccgttgt aaagtttttc tcggcgctct tcctccattt gaacttcaaa tagcttgcca
    11701 gcttcataaa attcgcggat ctcgtctgta tctttccaat aaccgacagc taatcctgct
    11761 aaaaatgctg cccctagcgc cgttgtttcc aagttttctg ctcgttggat cggtacattc
    11821 aagatatctg tttggaattg catcagatat tcattgtttg ctgcaccgcc atctactttc
    11881 aatacaggaa tcttgattcc agtgtcatct tgcatcgtat cgatgatatc tctcacttga
    11941 taagcaatcg actgtaaagt agctttaatg atatcttctt ttgtcgtacc acgtgtcaag
    12001 ccaaacatag cccctcgtgc tgcctgatcc caataaggag cacctagccc aacgaatgca
    12061 ggaacaacat aaacctcatc ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta
    12121 gctttttgca acatttgaag tccatctctc agccattgaa tcgaagaacc agccacaaaa
    12181 atacttcctt ctaaagcata gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat
    12241 aaattatttt tggataattg cggtttttca ccggtattca tgacaataaa tgaacctgta
    12301 ccataagtat tcttgaccat gcctggttcg aatgccattt gtccgaataa tgcggcttgc
    12361 tggtctcctg ccatccctga aattggtgct tcgcttccat aaaaatgata accttgcgta
    12421 taaccataaa cttctgagtt agaagtgact tttggtaaca tcactcgagg aatatttagt
    12481 aaatctaaaa tctcctgatc ccaatctagc tgatggatat tgaacaacat cgtccgactg
    12541 gcattcgagt aatcagtaac atgtgcttgt ccatcggtca gtttccatac taaccaagaa
    12601 tcgatcgttc caaacatcaa ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga
    12661 tcaagaatcc agcgtatctt cgttgctgag aaataggcat cgatcactaa ccctgttttt
    12721 tcgtggaaaa attcttgata gccttcattt tttaagttat ctgcaattcc agctgactgt
    12781 ctggattgcc aaacaactgc atgatagatt ggacgtcctg tatctttttc ccatattact
    12841 gttgtttcac gctggttcgt tatgccaatc ccagcaatct gatttggttt gatccctgat
    12901 tcgatgaacg ctccggcaat aactgactgt acagaattcc agatttcatt tgcatcatgt
    12961 tccacccaac tttcttgcgg gaaatattgc gtgaactctt tttgagaact gccgatgtgt
    13021 cttcctttct tgtcaaagat aattgctcgt gagctagttg taccttggtc aatcgccatg
    13081 atatacgttg attctgtcat tgtgattccc tccattatat gcaaacgctt tctttaacta
    13141 ctcttatcat actactgttt taaaaaaaga aactgtcaaa attttattct ttttttcttt
    13201 ttttgacagt tttttattat gaagctattt tttaatcaat tcttttcttt tttcagcgtc
    13261 tctattcttc ttttcaatcg aataatgtcg taaggtgacg caaattctga cagataatat
    13321 tcacactgtt ttttgtttct ctggcaaaat gtgatcacca aatcatattc tttaccttct
    13381 tcataacttc caatctcaat accgccgatt cccctcagtt ctctgatcag atattgttga
    13441 aaagcaatac gataaatcgg cagactttct aaatcgattc caatcactac tggttttgca
    13501 atataaaaat cgatcatcaa aagaacattg gcatactgga tcatcaggta actaagctca
    13561 ctatcttcag ctcttttcct cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt
    13621 aactgtgtta aggaatgccc gagcattttc tgctgctgtt ccaaaagccg atcacgctca
    13681 tagacttcga tctttccaac aaagaaaaac cattcattgt tgatctggga aatttggtag
    13741 cctactgcct tttcttcttt gattttcaaa cgacgaggac gataatgcca taacatcgtt
    13801 tcccgaatgt aagtatctaa aacagcggtt ggtaactttt tgctccgtgt caaatcatac
    13861 tgataaaata tttcttcgtc tactatcgaa aaagaaacaa aaaagctgta aaacatcatt
    13921 gtttcgtata cattcagttc tgctgctgtt cgactaagat gcatcacgat gattggatcg
    13981 attgcttgat aaagccggtc cgactgatag agtaatttct tttctttcaa agttgcgtac
    14041 gtactttttt cattcaatag tctttttttc gtaatcccga gccagcaagc aatctgtgct
    14101 tctgctgatg ccgtaaacgt tgtttctaat acacggtcca atcctagaat gaacggtctt
    14161 ttttctggtg tattttgcaa aaaaagcggt cgctgatctt ccgggatata ttggaatagt
    14221 tcataataaa aatatcgtat ctgtagctct tctccgtata attgaccgtt tttgatttgt
    14281 aatccaaatt cctctaagag ctggttcaat tctttgatct ttcgaaaaac agaagactca
    14341 ctgatattaa acgctgtcgc taatcgaacg atggatactt gctgatgttc taataaatag
    14401 acgagcatct taaacttcaa gctttcctgc aataaaaaca atagaatttt ttccaaactt
    14461 acatcttcgt ccaacacaag cagcgcctga tactcatttc gctgtacttc tacttttttt
    14521 tccatcattc ggccaatcaa ttcaatatct tccaaatatt gatcaaacga ggcttttgac
    14581 agttggacat gctctctcaa accattgata gaggctgttc ccccttgcag aacaaggttt
    14641 cgcaaaatca ggatctgtaa ctgctctttt ctttctaata atgcttcaat cttcataggc
    14701 agtattcgcc acctttgacc acaccatatc tggcacacgg tattctggct ggaaaaatag
    14761 ctcatgttct tctaactgtt caaacgtcca tccttcttct actgcaattg tcagttggtc
    14821 aagaaggtgg aacatctctt ttttgctttg gagctgtgct ccaagaatct ttttttcaga
    14881 atcaaataga aatttgagtt taactttgct ttgatttatt gctgtaagct tgtagtctac
    14941 gtatcctgtt tcaatttttt ggggataaaa aatgctctct tcttctgtca aacctacgct
    15001 tcctaagaaa tagccaaacc actgattgcc gactggacga taggttccct gatcctttgt
    15061 ctgtgagcca ctaattgtct tgctgacatt tcgtgctgta cggacagcgt tatttactag
    15121 agaaacatac attttttcat ttgttggacg aaaagttact tggatcgcat cgccaatcgc
    15181 ataaacgtct ttttgagacg tttgcaagta gtcatctacc cagatcgttc catcatcatt
    15241 caatgttaat tgttcttccc acatctgatt gtcgggacga gaatgattag ccagcaaaac
    15301 aagatcatag ttctcctttc ttttttccgt ctctacaata gcggtttcat tttcggtcaa
    15361 agacacaact tgttcttcta aaaaaagtga aagcccttga tttttcattt cctttactaa
    15421 cggttctgtc atttcaggat ctagataacg aaaaagaatt gactttcggc tttcgtaaag
    15481 atggatttgt ttttttcttg atgccaaccc ttcagctatt tcgattcctg cttgtcctgc
    15541 accaataatt gcaattttct ttgcattcat gatcttttgc tgaagagccg ttacgttttg
    15601 tatcttttta actgggtgaa taagcgttga ttctgcagaa atatttcgaa acttttgatt
    15661 tgaaccagtc gctagaatca aacgatcaaa atccaatttt tttcctcctg ctaaaacgac
    15721 ttttttttct ttcaatccta ttactgtctt ttctgtgtaa acatggattc gatagcggtc
    15781 agtcagttct tctttcgtga tccaagaaag ttgtttttca ctggtaacct ttccttttaa
    15841 tatcaaattg aacccgctgg gaatccatcc taacttcgat tgcttttcaa tcaaatatat
    15901 ttcacttgtt ggcatcaatt ggcgaagata aatagccgca tgaatccctg caaacgaccc
    15961 gccgacaatt acaatgttca tctgcattcc tctttcttat aaggtgttct actgctaact
    16021 tctcaatcag aaaacacagt attcataact cgctttcaac cttatgatac atagcgatgc
    16081 agcagaatca accctctcca tcataccgtg ttctaggtct gcttctcaat aatgtactag
    16141 tggacctaat tgaagagtat catttctgaa gagatgtttc aaaaaatgta ctcttataaa
    16201 agaaaaaaac tcgcctttta cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag
    16261 acgagtttta gcgtgtgtaa tgtaatagtt agaatagaca catcatttat ttttaattct
    16321 ttaatgcttc aacaactgct tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa
    16381 gaagccgccc atcaatttga accacttctg aaaaagctgc tccagactga tagtttgctc
    16441 ctttttcttt caaagcatct tctgctaaga atggtacttc attcgttgta ccatttgctt
    16501 cctcttcttc attactgaac ccagtcactg tacgtccagc taaaatagat tttccagact
    16561 catctgtaac tggcaacaat cctacaacac cgtgacaaac agctgagacc agcccttcat
    16621 ttcgatagat ttgtgaagcg actttcccaa gatctattgc ttctggaaaa tcccacattg
    16681 ttccgtggcc accagcataa taaaccgctg cataatcatt tgcatccaca tcatcaggac
    16741 gtaaggtatt tcccaaggcc cgatttctga aatcttcatc ttcataatag gcccaatcga
    16801 tttcatccat attcaaaaca ctttgcggtt ctaaaggtac atatccgcct ttaggactga
    16861 caaaatctgc atcgatccca ttttttacta atacatcata aaaatgagtc agttcactta
    16921 accacaaacc tgtaggacgc tcgatctttt cgtacttttc tacatttgtt aatacgatca
    16981 atactttcat tttcccatcc tctttctata aaaattgcgt tattgataac gtgctttcaa
    17041 gaaattatat aatgggcttg gcaaataaag ctctttatat gctttttcaa aaggtttcgt
    17101 tacaaattca tcctcacggt tttccaataa tttctctgcc cagtcaggat cgacgatcat
    17161 ttgctgtcca attgctgcaa gttctgcata tttcaatacc tgctctacat cttcgcgatt
    17221 gcggatccca ccgacaccaa ctaaagggat ctttccttga atcgtgttat ggaccacttc
    17281 aagaatcgat ttatcgttgt atttatcaga acgagctatt cgatcataca tatttaatga
    17341 aacatgcaga taatctagtt ttgactcacg taatcgttca agaagccaga tcgtgtcttc
    17401 aaaacggata cctggtgttt catattcttc tggtgagaaa cgatacccta cgatgaatgg
    17461 ttttttagca tattgttcaa ccgctgagaa aaccgttgtt aataattctt caataaaacg
    17521 ataacgattt tccaagctac cgccccattg atcctctcgt ctattcgaat gcggagagaa
    17581 aaattgttgg atcaaatacg tatttgctcc atgcaactcg attccatcaa atcctgattc
    17641 aatcgctcta cgtgtggctt tgccaaatgc ttggattgtt tcaacgattt cttcttcact
    17701 cattgctcgt ggtgtttccg catcaggacg ttttgctgca actgcacttg cagatacagg
    17761 ctgttctcca gataaagttg ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat
    17821 cgcttttgcg ccttctcctt ggattgcttt cgctagctca cttaatcgag gaatcatttc
    17881 atcatgagcg atgctgagtt cacctggcca gccttttccg atatcttgga catttgcagc
    17941 accagtaata acagcaccaa gacctttaga acgttgttta taataagtga tctcatcttc
    18001 ggtgatcact ccattgaaaa agctgagttg tgtcgtcata ggcgacagca tcaatcggtt
    18061 cttcaattga actcctgaag gtaaagtaat tgattgatca aattgtgaca ttgtcattcc
    18121 tcccataatt cgctctgatt aacctatcga gaatcatcta tcattaaacc actcgattag
    18181 ttgtacatgc aactaatttg cttaaaaaat tttatttttt ttgtaactta tctgttgcat
    18241 cattcattgt ctgggctaat tctttacttg cttcatttcc caaaactgct ttgtatgact
    18301 gatgaagttc ttcccagcta ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa
    18361 gataaactaa tgacaagcga ccttcgctaa ctgttttgac gtatccttta tattctaatt
    18421 tttcaacaaa acgagcaatc gttgatgggg cggaatagag catttgtgca atatcttttt
    18481 gtggtattcc atctttttct tttaatatca ataaaataaa accataagtt ggtgccagac
    18541 ctgtccttct aaacgcttca tcagctaatt tattcaacat gcgatctagc tttttcacgc
    18601 taaagaaaag acattcatct aacgcttttt caattggctt ttccatttca tcccctccat
    18661 tggcgtacat atcatagtat gatttttatt ttttgtcaaa aaacacggaa taatgtccgc
    18721 ctcctctgtc tagatttgaa aaaaagaagt agccatcttc aaagaatttc ggcttgtttt
    18781 cgacaaaact ccttctggaa caccgtgaat cagaggttgt gaaaaagtcg tttagttttg
    18841 agtatgaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat
    18901 accgaaaaac tggcttttga acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca
    18961 tcaagtaata agaacagcca aacaaaaata gttcctcata ttttgtcgtt ttttgagcta
    19021 atgccgatgg tcagctgatt gtacaccgtt tattgagagg tcgtgagcct gccgttgaac
    19081 tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gttttatgac tatggtgact
    19141 tgtgatcgag gagttggcag gagaatactg tttattcgta aagaaggggc tgtgacaaga
    19201 cttttgtcac agtcccttat ttccttttat tataggattt cgccatttga ttggatgact
    19261 tgtctgtacc aatcaaatga ttttttcttt aaccgtttct ttgtgccatt cccttcgtca
    19321 tcttgatcaa catagatata tccataacgt tttgacattt gtgaagtaga agcgctgacg
    19381 atatcaatgc agccccacga cgtataacca aataaatcta ctccatcttt gatagcttcc
    19441 ttcatctgct caatatgctg cttcaaatag tcaatgcggt aatcgtcttg aacggttcca
    19501 tcttcttcta ctttgtctat agctccgagt ccgttttcag ctatgaacag tggtttttga
    19561 tacctatcat ataattgatt caaagcaatt ctcaaaccct taggatcaat ctgccagccc
    19621 cattcacttg atggaagata cggattttta attcctccag ttattaagtt tccttggacc
    19681 ccttcccaat tttctggatg agcactgact gcactagaca tgtagtagct aaaagaaaca
    19741 aaatcaacag ggtaggctgc taagatttct tcatcaccta cttctatttt taagttgaca
    19801 cctttttctt caaacattcg ttgcgtatac gctggataat atcctcttgc ttgaacatca
    19861 ctaaagaaca aagaagttct ttgctgctca attgtttttt gaatatcatc cggatcacaa
    19921 gaatacggaa caagaagctg atagctgatc atacaaccga cttgtgaacc tttgataatt
    19981 tcatgacaag cttttgtagc taaagcactt gctataaact gatggtgtgc accttggtat
    20041 ttcgcttgtt ccaaatgggg atggttttct tcaattactc ctacgctaac aaaagggtga
    20101 tgttttacac agttgatctc attgaaagtc atccaatatt ttactttatt tttatagcgt
    20161 ttgaatactg tttcagcgaa acgaacgtaa aactgaataa gctttcgatt aggccatcca
    20221 ccatattctg taaccaaata taacggcatt tcataatggg aaagagaaac gactggctca
    20281 atcccatatt ttagacattc gtcaaacact tgatcataaa aaaataatcc cgcttcattg
    20341 ggtgtttctt catcaccatg aggaaaaatt cttgtccatg cgatagacat ccgatagcat
    20401 ttaaaaccca tttcagcaaa taaacggatg tcttctttaa agtgatgata gaaatcattt
    20461 ccgtgtcttt taggataata atacttgctg ttgggatcct gcgccgcttt tacttgctga
    20521 tgcgtcatca tatgccattg atctgtccat ttttctttag gtaaatcgct gtcgaatgta
    20581 tacacatcag acacagataa tcctttttca tctacatccc atgcaccttc acattgattg
    20641 gccgctgtcg ctccgcccca taaaaatcct tctggaaatt ttttcatatc gatcccccta
    20701 aacttgctag ttttcttgtt tgattatttt tcttatgact agtaatgata tttttgaatc
    20761 tactactatt tgtaacaata accattgtgg tcggatcata tccctctttg attaattctt
    20821 gcaaatctac ttccatcaat aaatcgcctt tacgtacttt ttttccatca gatactgcac
    20881 tttgaaagta tttgccattt aaattcactg tatcaatacc gatatggatc agtatctctg
    20941 ctccttcttc actgactacc ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc
    21001 cgtttacaga actgtagatt tttccgtccg ttggaatgat cgcaaaccct tctcctaaag
    21061 atccactagc aaatgttgga tcgtttactt cagataaatc aattatttca cctgctgcag
    21121 gagtacttaa cactatatcc tttgaggatt cttcttcgat ttcatccttg tagccgaaaa
    21181 gaaaagttag catacaggaa ctgatcatcg ctaagaaata actagcaact agccaaacaa
    21241 atccttcacc aatataaatc gggaaagaca aaatacttct tgtagcaact gccaaagcac
    21301 cggctcctgc ggcaccagct actccaccaa atatagctcc acatatacaa gcaatgtaaa
    21361 atggtcgttt atatttaaga gtcacaccat aaacagcagg ttcagtgatt ccaaataatg
    21421 ctgtgataaa tgcagataaa gataaacttt ttactttctt atttttcgtt ttcaaaaata
    21481 ccccaagtac tgctcctgct tgcgcagcta cggcagtcat acatgctgga cctagcgtat
    21541 cacgaccgta gaaagaaaga ttgttggtca ttactggtac aagtccccaa tgtaacccaa
    21601 aaatcactaa cacttgccaa aatccgccga taacagcacc agataaaaca gggctaaagt
    21661 tataaacagc taaatatcca gctgcaatca gctgacttgc ataatctgca attggaccta
    21721 caactaataa aataattggg accatcacga tgatacaaat gaatggtgtc aataaatttc
    21781 gaatactttc gtgcaagctt ttgcgaatct taggctcaat ataagaaaga acccatacag
    21841 ctaaaattgc tggaataacc gtacttgtat accttgctag aatcacgggc atccctagaa
    21901 atcgcaaggt tatgctatca ctatatgcac tgacaatcgt aggataaacg agagctgctg
    21961 caataaccat agcgatgaaa cgatctgtat tgaacttttt tgcagcagta tatgccaaaa
    22021 atataggcaa gaaatagaaa aatgcatcag ctgctgcatt caaaatgata taagttcctg
    22081 attcagtagt taataaattc aaactcgtac ataaggcgag tactcctttg atcaaaccac
    22141 ttcctgccaa gacgcctaaa gtcggggcaa aaacagcggc gatcatgtct ataaaagagt
    22201 ttaaaatgcc ttttttctca gttcctctct ttccactaga tgcatcatct tctagtttca
    22261 tttgtttgga taatacgtca tacacttcat ttactgcatt tcctactacg acttgaaaca
    22321 ttcctccagc ttcgactaca gtgatcacac cttctagttg ctctaccttc tccttatctg
    22381 ccttcttatt atcttttagg gtaaagcgta accttgttgc acaatggtaa acattgctga
    22441 cattttcttc gcctccgaca aatcggacaa tatctttcgc taattgtgaa tagtccattt
    22501 ttacttcccc cttgttaatt aatgccgtag caatccttct tacaagataa gtatagttct
    22561 tagaaaacgg tttctccatt caattagtac cccttctaat agcgaacgaa actgtacaat
    22621 agtgtgtatg gtttacttag ccgagcaaat cactcactgc ttgaaagaaa acaagtatat
    22681 cgtttctctt atagtcaaag gagacaaaaa aaacacgctt gatcttcctt tgctgatgta
    22741 taagtggtat ctcaagctga tactctgtga tttttgtttc tacttggctt ttgataagac
    22801 agcacaacaa agtctcggca tttaacggaa gcataactag gtcagaacta ggagagatta
    22861 gttgctgaat ggaatctatc tttttatgga tttttatttg attttcttct ttgtagtaat
    22921 gatcaagcag ttttgttttc tttttttctt caactatggt cagcttttca cgcaaaatct
    22981 gataaatctc ccaagattca aactcatcta gaacactagg tagataaatc aaatcatccc
    23041 actcaaaaat aagctcttgc tcagtagtca aaattgcatc atattccgtt attttgtttg
    23101 attgattttt gaaaagatac actggttcta tccggcattc tttgatccac atgttcatac
    23161 tttgttgaat ctgttttttt agtgtattca aaatactaat tggctggtta ctaacaatca
    23221 gtatctcccc ttgttttcga aaaggattca acgcttcggc aagatacaaa acaaaataag
    23281 cgctttcagc taaattggga cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat
    23341 aaatttctaa aggataacgg gagatgatct tttgtacaaa atgattcatg gtattatgtc
    23401 cggcatccat cctcgttttc atttgtttga tatgggttag caaaaattct gtttcatgga
    23461 acaataacga aacaggaaga tctaaaaaac gaataatcgt ttgctcaaat gctcttagat
    23521 tcttctctat ttctttgttt ttttcatcat taatcatcaa ataatttaac tctaatagtc
    23581 gattttcaat tgcttgtttt tcttgattgg aaaaagaata gggtagttct gatgataact
    23641 tttttgtgag ggtctctacc atcggaataa ataatgtata cctgtttgtt ttttctaaaa
    23701 gttcttctct tcgaagaatg gtcaaaacga gataccgaga aaatcgtatg aagtcctctc
    23761 ctgaaacaat ataattatat gatataagaa cctcttttaa tgtcttttgg acagtcggca
    23821 tgatattgga aaaaattgta ctgatttgtt gaggaaacct tgcgtgttgg atcaccgttt
    23881 ctgttccaac aagagataag aaaattcttt ttgaattttc ttcagcaatg atttttagtc
    23941 cttttgtcga cgaaacttcc agttctaatt taggattgcg ctcgatccaa cgcaaaatcg
    24001 tttttatttg taatgatacg gctgtttttg aaagaaagaa aatgtctgct atcttttgca
    24061 tactgacata ccctttttga aacaataagt acgtcacaat agttgaagct cggaaatcca
    24121 tgctggaata gattttatgt ttcgtcacct cttgtatgat ttgctgttta gtttcagatt
    24181 caattgattc taaaaaataa cctgaacgtt tatttgactg gataatgatt ttcttctcgt
    24241 agatagcaag catatttttg atttcagttt ggatcgtttt cttgttggta cctaaagctt
    24301 cagctaattc ttcgcttgtt atccaatgat tcttatttct taatactaca agaatttgga
    24361 gttgtcgttt atttaagtcc atttgcttac acctcctctt tgttataaac atacaggaaa
    24421 aacctctcaa atgacaatag tcacttgcag aggtttaaaa atcatttgaa gaaagatcta
    24481 tattcaaggt ttttaccatt agactggata atttgtttat aatattcaaa actatctttt
    24541 ttcaatctcc taaagcttcc gctcccatca ttttgtctat ccacataaat aaagccatat
    24601 cgcttcctca tttcccctgt tcctgccgat acgatatcga ttggtcccca ccataagtaa
    24661 ccaatgattt ggcaaccatc ttttattgcc tcagcaattt cctgaagatg tatttttaga
    24721 tactcacaac gttttggatc gtggattcga ttcaattcat ctggtgcttc atctagtcca
    24781 atgccatttt caactatgaa taatggtaat tcataacggt ctgtcaattc attcatgaca
    24841 taacgaaacc ctagaggatc aattggccaa ctccaaggtt cgggagattt ttccttcaaa
    24901 aagggattat cgaaaccatt tgctccgcca gtatctacgc gcatttctgc ttctttttta
    24961 tagacagcgc tacgataata actaaatgca atataatctg ccgtgttagc ttgcaagcac
    25021 tgttcgtctc catctaacat tactggtgca cagtcttctt ccttccatgt cctgtaaata
    25081 taagctggat aatgccctcg gcacatgaca tcggagaaaa accatgaatt gcgtttaaat
    25141 tgcagtgaac caaatacatt ctctggatca cagtcatatg cataagtcgc taatgcagaa
    25201 aagctacaca taatacccat cttagcattt ggatcgattt tctttaataa ttgagtagca
    25261 cgggcgttcg ctagaaacat atgatgacag gcttgataaa tcactttttt tgttagtgta
    25321 gcaatgggtt caagtttatc tctagcaggg aatgtaacca atccagcagc tgcataaggc
    25381 atacgaaaag cattattgat ctcattaaag gtcatccaat atttcacttt cccttggtag
    25441 cgttcaaaga cggtctgcgc ataaaattca aaaaagtcga ttaattttcg attttgccac
    25501 cccccatact ctcctaccaa tgctaagggg gtttcataat gactaagagt aattactggc
    25561 tcgatatcat atcgatttaa tgtattgatt agtctgtcat aaaatacgag accagcctca
    25621 tttggcttct gttcgtctcc tcttggaaat attcgtgccc atgagatgct tgttcgaaaa
    25681 gcttttaaac ctaactcctt cagtaaacga atatcctctt caaatcgatg ataaaaatca
    25741 atcgcttcat gagaaagata atggagtgat tcgtctaaag caatctccca aatctttttt
    25801 tcttcattcc actgaatact aggatgctgg ttcatgatcc cgataagcgt atctgtcaca
    25861 ttaggtaatt tgccgtcttc caaccatgca ccttcacatt gattagcagc tactgctccg
    25921 ccccataaaa aattttctgg aaatatttcc tgatccataa ctatctctcc tcgctatcaa
    25981 caatttctta cttgaagtat agctcgattc atcaaaacaa gatattctaa ctatttcggc
    26041 agaagagaag aaaaaaattg aatgctactc ttcattttta atatttatat ataacaaata
    26101 cataagacga tccccgaaag aaaacactcg ttaaccatct aagcaaacga tttattttac
    26161 ttccaacaca aatgcttcat aaggtcgtaa atcaactttg ccgtctggta actgtttcag
    26221 aggataattt gacacaataa cccttaaagc ttcacgtgga atagtgaacg tctgcttttc
    26281 actggacata ttacaaataa cgagccattg ctggtcttca tatatgcgag aataggcaaa
    26341 gacatgctca ggcgtctttt ccactaattc atagtctccc cagacgatga tgtcatgttt
    26401 tctacgcaaa tcgatcaatt tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc
    26461 ttcttctgca ttgatttctt tataatttgg attgactcga agccatggaa ttccagtagt
    26521 aaacccagca tgttcacggg cgttccattg cataggcgtc ctagcattgt ctcttccttt
    26581 cgccttgatt gattccaaga tttcctcttt tgtaaatcca gaagaaatcc tttcgttata
    26641 catattgatt gtctcaatat cttttgcttc cgcgatatca tcgattgggc aattcgtcat
    26701 gcctaactct tctccttgat agatataagg ggtgcctttc atcatatgga gcaagatagc
    26761 aaacatcttg gcactttctt ttcgatacac atcgtcattt ccccaacgag aaacaattcg
    26821 cggaagatcg tgattattcc aaaataaact attccatcct tcatcaccta aagatgtttg
    26881 ccatttggat aatacttttt tcagttcaga gatactcaag ggtttcaaat cccatttgtc
    26941 tttgccttct tgctgatcta atcctatgtg ttcgaattgg aagaccatgg ataattcatt
    27001 tcttttagga ttggaataaa gtttagcgat ttcggtagtt gctccccatg tttcgccaac
    27061 agtcatcaga tttttgtcac caaaagttgc ttgattcatt tcctgaagat actcatgcag
    27121 tttaggtcca ttacttgtga tttctttatc cggttctttg ccaatcatat cgatcacgtc
    27181 catacgaaag cccccaatac ctttatcgat ccagaaattc atcatttgat aaatctcttg
    27241 gcgaacctgt ttatttttcc aattcaaatc aggttgacgc ttgctataca agtgcagaaa
    27301 atattgcccc gttttttcat ccaactgcca agcagaacca ctgaatattg agcgcaatct
    27361 attaggctct tttccattga ccggatcacg ccaaatataa tagtcccgat aaggattttc
    27421 tttattttta cgggcctcaa tgaaccacgg atgttcatca gaagtatgat tcaccaccag
    27481 atccatgatg atacggatat ttcttttctt tgcttcttca atcagttctt ccatttcttc
    27541 catcgtaccg aattctgcca tgattgcttg ataattactg atatcatacc cattgtcatc
    27601 attcggagac tcgtatactg gagaaagcca aattgcatca atacctaaat acgccagata
    27661 atccaacctg gaaataatcc cccgcaaatc tccaatacca tcttgatttg aatcttgaaa
    27721 acttcttgga tagatttgat aaacaactga atggtgccac catttttgtt ccatgatttt
    27781 cctcctataa atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta
    27841 aaaattgtaa cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa
    27901 aaaacagctg aggaaattcc ctcaactgtt ttttgaaagg attgatttat gcagggaaga
    27961 atgctgattt cacaacttct ttataaacgg tgttcactca gctgtccctt ggtattagct
    28021 caaaaaacgg caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag
    28081 aacgctgatt tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac
    28141 aaagaacaga ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt
    28201 aattggatag aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat
    28261 gccgatcaag atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg
    28321 caaagattgt gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt
    28381 aacaactgat agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat
    28441 agctgtgata cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc
    28501 aacaggagaa gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc
    28561 tttccctaca cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac
    28621 taaagttcct gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat
    28681 ttgaagagaa ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact
    28741 tggtgctgcg gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat
    28801 cgttgtagct gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc
    28861 agttacgact aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc
    28921 tactaatgta gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat
    28981 aaatagaccg ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat
    29041 catttcccgc agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc
    29101 ggctaatgct gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc
    29161 attgatccca agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac
    29221 acagccgatc gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga
    29281 agcacctaat acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa
    29341 tcctgccagt acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag
    29401 tattttttcc ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa
    29461 atttcgattt attaataaaa caacgtgttt tatcatatac taaaacaaaa cataagtaaa
    29521 caaaaaattc ggattttaag caaaatccga acgttccatc tgattaaatc cctttatctc
    29581 aaagttcatg aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc
    29641 ttcattctag tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt
    29701 ctttgatgaa ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat
    29761 cgaccatttt tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa
    29821 gcttctcact atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat
    29881 ccactgtttt tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa
    29941 ttgccttcag ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct
    30001 ggttcttcca tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc
    30061 ggtggtccta atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat
    30121 tctaattctt ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt
    30181 tcagaaattt ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg
    30241 acataaagca tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt
    30301 ctttcctgtc tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat
    30361 actattcaag aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt
    30421 acaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct
    30481 tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt
    30541 ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat
    30601 tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc
    30661 cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt
    30721 gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct
    30781 aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac
    30841 aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga
    30901 tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg
    30961 gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg
    31021 taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct
    31081 tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat
    31141 acttcccaga aattggtatc cactattact tttttcatct gttcacacct cttgattaga
    31201 ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt
    31261 cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc
    31321 aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta
    31381 gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt
    31441 ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac
    31501 ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt
    31561 ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat
    31621 tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat
    31681 ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc
    31741 ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta
    31801 aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg
    31861 agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga
    31921 gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta
    31981 atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt
    32041 cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta
    32101 aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga
    32161 tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac
    32221 ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact
    32281 agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt
    32341 gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc
    32401 ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc
    32461 accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt
    32521 ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc
    32581 ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc
    32641 aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt
    32701 gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc
    32761 ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg
    32821 gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga
    32881 aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt
    32941 agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata
    33001 taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac
    33061 ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac
    33121 caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca
    33181 gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca
    33241 caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg
    33301 aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag
    33361 ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg
    33421 taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg
    33481 tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct
    33541 ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga
    33601 cgatctgatt cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt
    33661 aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt
    33721 tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa
    33781 tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc
    33841 aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca
    33901 tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga
    33961 ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt taattggact
    34021 ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc
    34081 tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc
    34141 tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt
    34201 gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac
    34261 atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa
    34321 ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa
    34381 tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc
    34441 ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata
    34501 ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata
    34561 catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg
    34621 gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac
    34681 taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga
    34741 acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa
    34801 agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata
    34861 catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa
    34921 tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac
    34981 gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga
    35041 tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca
    35101 attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg
    35161 tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg
    35221 gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa
    35281 aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga
    35341 tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga
    35401 atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat
    35461 gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg
    35521 ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca
    35581 tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga
    35641 cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa
    35701 ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac
    35761 cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga
    35821 tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga
    35881 tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga
    35941 aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa
    36001 gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat
    36061 catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga
    36121 aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat
    36181 agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt
    36241 acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca
    36301 gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag
    36361 aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac
    36421 ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg
    36481 tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca
    36541 atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca
    36601 tgtt
//
LOCUS       DAJSVD010000016        36169 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSVD010000016 DAJSVD010000000
VERSION     DAJSVD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.016"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            258..533
                     /locus_tag="ON498_000690"
     CDS             258..533
                     /locus_tag="ON498_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3938987.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            546..2624
                     /locus_tag="ON498_000691"
     CDS             546..2624
                     /locus_tag="ON498_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR3938988.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2784..4670
                     /locus_tag="ON498_000692"
     CDS             2784..4670
                     /locus_tag="ON498_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3938989.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4682..5629
                     /locus_tag="ON498_000693"
     CDS             4682..5629
                     /locus_tag="ON498_000693"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3938990.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5649..6176
                     /locus_tag="ON498_000694"
     CDS             5649..6176
                     /locus_tag="ON498_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3938991.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6239..6892)
                     /locus_tag="ON498_000695"
     CDS             complement(6239..6892)
                     /locus_tag="ON498_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3938992.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7024..7866
                     /locus_tag="ON498_000696"
     CDS             7024..7866
                     /locus_tag="ON498_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3938993.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8024..8920
                     /locus_tag="ON498_000697"
     CDS             8024..8920
                     /locus_tag="ON498_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3938994.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8923..9636
                     /locus_tag="ON498_000698"
     CDS             8923..9636
                     /locus_tag="ON498_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR3938995.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9656..10174
                     /gene="msrA"
                     /locus_tag="ON498_000699"
     CDS             9656..10174
                     /gene="msrA"
                     /locus_tag="ON498_000699"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3938996.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10171..10398
                     /locus_tag="ON498_000700"
     CDS             10171..10398
                     /locus_tag="ON498_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR3938997.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10550..11101
                     /gene="lepB"
                     /locus_tag="ON498_000701"
     CDS             10550..11101
                     /gene="lepB"
                     /locus_tag="ON498_000701"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3938998.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11184..11684)
                     /locus_tag="ON498_000702"
     CDS             complement(11184..11684)
                     /locus_tag="ON498_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3938999.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11963..12361)
                     /locus_tag="ON498_000703"
     CDS             complement(11963..12361)
                     /locus_tag="ON498_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3939000.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12621..13481
                     /gene="ylqF"
                     /locus_tag="ON498_000704"
     CDS             12621..13481
                     /gene="ylqF"
                     /locus_tag="ON498_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR3939001.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13474..14241
                     /locus_tag="ON498_000705"
     CDS             13474..14241
                     /locus_tag="ON498_000705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3939002.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14297..15154
                     /gene="dprA"
                     /locus_tag="ON498_000706"
     CDS             14297..15154
                     /gene="dprA"
                     /locus_tag="ON498_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3939003.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15276..17354
                     /gene="topA"
                     /locus_tag="ON498_000707"
     CDS             15276..17354
                     /gene="topA"
                     /locus_tag="ON498_000707"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3939004.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17323..18699
                     /gene="trmFO"
                     /locus_tag="ON498_000708"
     CDS             17323..18699
                     /gene="trmFO"
                     /locus_tag="ON498_000708"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR3939005.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18897..19802
                     /gene="xerC"
                     /locus_tag="ON498_000709"
     CDS             18897..19802
                     /gene="xerC"
                     /locus_tag="ON498_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3939006.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19839..20387
                     /gene="hslV"
                     /locus_tag="ON498_000710"
     CDS             19839..20387
                     /gene="hslV"
                     /locus_tag="ON498_000710"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR3939007.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20401..21801
                     /gene="hslU"
                     /locus_tag="ON498_000711"
     CDS             20401..21801
                     /gene="hslU"
                     /locus_tag="ON498_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR3939008.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21819..22610
                     /gene="codY"
                     /locus_tag="ON498_000712"
     CDS             21819..22610
                     /gene="codY"
                     /locus_tag="ON498_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR3939009.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22692..23567
                     /locus_tag="ON498_000713"
     CDS             22692..23567
                     /locus_tag="ON498_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3939010.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23699..24337)
                     /gene="plsY"
                     /locus_tag="ON498_000714"
     CDS             complement(23699..24337)
                     /gene="plsY"
                     /locus_tag="ON498_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3939011.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24464..24898
                     /locus_tag="ON498_000715"
     CDS             24464..24898
                     /locus_tag="ON498_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3939012.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25064..27109
                     /gene="parE"
                     /locus_tag="ON498_000716"
     CDS             25064..27109
                     /gene="parE"
                     /locus_tag="ON498_000716"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3939013.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27121..29571
                     /gene="parC"
                     /locus_tag="ON498_000717"
     CDS             27121..29571
                     /gene="parC"
                     /locus_tag="ON498_000717"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3939014.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29848..32079
                     /gene="pflB"
                     /locus_tag="ON498_000718"
     CDS             29848..32079
                     /gene="pflB"
                     /locus_tag="ON498_000718"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3939015.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32323..33084
                     /gene="pflA"
                     /locus_tag="ON498_000719"
     CDS             32323..33084
                     /gene="pflA"
                     /locus_tag="ON498_000719"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR3939016.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33169..34095
                     /locus_tag="ON498_000720"
     CDS             33169..34095
                     /locus_tag="ON498_000720"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR3939017.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34277..35170
                     /locus_tag="ON498_000721"
     CDS             34277..35170
                     /locus_tag="ON498_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR3939018.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35304..35429
                     /locus_tag="ON498_000722"
     CDS             35304..35429
                     /locus_tag="ON498_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3939019.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35563..>36169)
                     /locus_tag="ON498_000723"
     CDS             complement(35563..>36169)
                     /locus_tag="ON498_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939020.1"
                     /translation="FLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDITE
                     AEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFN
                     HNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALM
                     PLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        12687 a         6203 c         7610 g         9669 t
ORIGIN      
        1 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
       61 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
      121 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
      181 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
      241 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
      301 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
      361 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
      421 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
      481 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
      541 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
      601 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
      661 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
      721 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
      781 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
      841 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
      901 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
      961 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     1021 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     1081 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     1141 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     1201 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     1261 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     1321 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     1381 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     1441 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     1501 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     1561 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     1621 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     1681 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     1741 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     1801 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     1861 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     1921 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     1981 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     2041 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     2101 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     2161 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     2221 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     2281 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     2341 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     2401 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     2461 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     2521 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     2581 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     2641 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     2701 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     2761 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     2821 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     2881 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     2941 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     3001 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     3061 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     3121 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     3181 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     3241 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     3301 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     3361 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     3421 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     3481 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     3541 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     3601 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     3661 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     3721 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     3781 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     3841 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     3901 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     3961 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     4021 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     4081 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     4141 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     4201 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     4261 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     4321 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     4381 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     4441 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     4501 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     4561 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     4621 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     4681 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     4741 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     4801 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     4861 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     4921 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     4981 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     5041 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     5101 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     5161 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     5221 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     5281 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     5341 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     5401 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     5461 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     5521 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     5581 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     5641 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     5701 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     5761 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     5821 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     5881 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     5941 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     6001 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     6061 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     6121 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     6181 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     6241 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     6301 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     6361 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     6421 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     6481 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     6541 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
     6601 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
     6661 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
     6721 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
     6781 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
     6841 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
     6901 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
     6961 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
     7021 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
     7081 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
     7141 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
     7201 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
     7261 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
     7321 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
     7381 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
     7441 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
     7501 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
     7561 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
     7621 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
     7681 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
     7741 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
     7801 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
     7861 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
     7921 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
     7981 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
     8041 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
     8101 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
     8161 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
     8221 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
     8281 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
     8341 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
     8401 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
     8461 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
     8521 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
     8581 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
     8641 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
     8701 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
     8761 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
     8821 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
     8881 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
     8941 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
     9001 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
     9061 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
     9121 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
     9181 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
     9241 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
     9301 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
     9361 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
     9421 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
     9481 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
     9541 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
     9601 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
     9661 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
     9721 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
     9781 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
     9841 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
     9901 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
     9961 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    10021 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    10081 tcaagactat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    10141 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    10201 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    10261 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    10321 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    10381 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    10441 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    10501 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    10561 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    10621 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    10681 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    10741 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    10801 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    10861 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    10921 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    10981 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    11041 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    11101 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    11161 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    11221 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    11281 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    11341 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    11401 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    11461 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    11521 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    11581 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    11641 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    11701 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    11761 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    11821 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    11881 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    11941 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    12001 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    12061 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    12121 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    12181 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    12241 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    12301 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    12361 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    12421 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    12481 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    12541 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    12601 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    12661 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    12721 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    12781 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    12841 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    12901 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    12961 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    13021 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    13081 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    13141 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    13201 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    13261 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    13321 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    13381 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    13441 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    13501 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    13561 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    13621 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    13681 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    13741 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    13801 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    13861 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    13921 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    13981 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    14041 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    14101 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    14161 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    14221 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    14281 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    14341 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    14401 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    14461 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    14521 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    14581 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    14641 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    14701 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    14761 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    14821 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    14881 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    14941 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    15001 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    15061 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    15121 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    15181 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    15241 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    15301 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    15361 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    15421 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    15481 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    15541 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    15601 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    15661 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    15721 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    15781 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    15841 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    15901 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    15961 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    16021 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    16081 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    16141 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    16201 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    16261 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    16321 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    16381 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    16441 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    16501 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    16561 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    16621 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    16681 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    16741 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    16801 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    16861 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    16921 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    16981 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    17041 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    17101 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    17161 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    17221 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    17281 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    17341 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    17401 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    17461 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    17521 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    17581 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    17641 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    17701 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    17761 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    17821 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    17881 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    17941 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    18001 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    18061 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    18121 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    18181 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    18241 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    18301 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    18361 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    18421 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    18481 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    18541 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    18601 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    18661 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    18721 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    18781 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    18841 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    18901 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    18961 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    19021 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    19081 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    19141 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    19201 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    19261 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    19321 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    19381 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    19441 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    19501 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    19561 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    19621 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    19681 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    19741 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    19801 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    19861 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    19921 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    19981 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    20041 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    20101 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    20161 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    20221 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    20281 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    20341 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    20401 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    20461 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    20521 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    20581 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    20641 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    20701 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    20761 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    20821 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    20881 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    20941 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    21001 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    21061 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    21121 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    21181 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    21241 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    21301 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    21361 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    21421 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    21481 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    21541 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    21601 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    21661 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    21721 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    21781 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    21841 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    21901 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    21961 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    22021 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    22081 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    22141 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    22201 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    22261 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    22321 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    22381 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    22441 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    22501 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    22561 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    22621 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    22681 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    22741 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    22801 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    22861 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    22921 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    22981 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    23041 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    23101 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    23161 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    23221 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    23281 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    23341 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    23401 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    23461 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    23521 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    23581 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    23641 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    23701 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    23761 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    23821 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    23881 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    23941 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    24001 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    24061 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    24121 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    24181 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    24241 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    24301 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    24361 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    24421 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    24481 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    24541 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    24601 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    24661 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    24721 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    24781 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    24841 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    24901 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    24961 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    25021 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    25081 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    25141 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    25201 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    25261 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    25321 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    25381 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    25441 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    25501 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    25561 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    25621 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    25681 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    25741 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    25801 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    25861 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    25921 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    25981 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    26041 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    26101 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    26161 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    26221 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    26281 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    26341 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    26401 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    26461 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    26521 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    26581 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    26641 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    26701 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    26761 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    26821 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    26881 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    26941 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    27001 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    27061 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    27121 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    27181 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    27241 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    27301 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca ccctcatggt
    27361 gaccgcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    27421 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    27481 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    27541 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    27601 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    27661 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    27721 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    27781 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    27841 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    27901 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    27961 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    28021 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    28081 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    28141 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    28201 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    28261 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    28321 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    28381 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    28441 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    28501 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    28561 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    28621 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    28681 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    28741 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    28801 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    28861 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    28921 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    28981 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    29041 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    29101 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    29161 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    29221 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    29281 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    29341 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    29401 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    29461 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    29521 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    29581 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    29641 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    29701 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    29761 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    29821 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    29881 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    29941 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    30001 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    30061 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    30121 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    30181 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    30241 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    30301 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    30361 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    30421 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    30481 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    30541 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    30601 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    30661 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    30721 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    30781 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    30841 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    30901 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    30961 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    31021 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    31081 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    31141 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    31201 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    31261 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    31321 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    31381 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    31441 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    31501 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    31561 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    31621 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    31681 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    31741 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    31801 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    31861 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    31921 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    31981 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    32041 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    32101 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    32161 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    32221 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    32281 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    32341 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    32401 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    32461 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    32521 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    32581 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    32641 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    32701 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    32761 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    32821 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    32881 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    32941 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    33001 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    33061 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    33121 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    33181 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    33241 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    33301 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    33361 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    33421 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    33481 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    33541 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    33601 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    33661 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    33721 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    33781 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    33841 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    33901 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    33961 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    34021 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    34081 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    34141 atataaaaag aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa
    34201 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    34261 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    34321 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    34381 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    34441 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    34501 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    34561 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    34621 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    34681 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
    34741 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    34801 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    34861 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    34921 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    34981 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    35041 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    35101 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    35161 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    35221 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    35281 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    35341 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    35401 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    35461 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    35521 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    35581 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    35641 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    35701 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    35761 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    35821 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    35881 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    35941 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    36001 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    36061 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    36121 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaa
//
LOCUS       DAJSVD010000017        35477 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSVD010000017 DAJSVD010000000
VERSION     DAJSVD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.017"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            36..974
                     /locus_tag="ON498_000724"
     CDS             36..974
                     /locus_tag="ON498_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR3939021.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            989..1819
                     /locus_tag="ON498_000725"
     CDS             989..1819
                     /locus_tag="ON498_000725"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3939022.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            1863..2888
                     /locus_tag="ON498_000726"
     CDS             1863..2888
                     /locus_tag="ON498_000726"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3939023.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            2903..3841
                     /gene="galU"
                     /locus_tag="ON498_000727"
     CDS             2903..3841
                     /gene="galU"
                     /locus_tag="ON498_000727"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3939024.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            3967..4407
                     /locus_tag="ON498_000728"
     CDS             3967..4407
                     /locus_tag="ON498_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR3939025.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4410..5012
                     /locus_tag="ON498_000729"
     CDS             4410..5012
                     /locus_tag="ON498_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3939026.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5074..6177)
                     /locus_tag="ON498_000730"
     CDS             complement(5074..6177)
                     /locus_tag="ON498_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3939027.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6387..7388
                     /gene="ccpA"
                     /locus_tag="ON498_000731"
     CDS             6387..7388
                     /gene="ccpA"
                     /locus_tag="ON498_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR3939028.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            7742..8137
                     /locus_tag="ON498_000732"
     CDS             7742..8137
                     /locus_tag="ON498_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939029.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            8134..8388
                     /locus_tag="ON498_000733"
     CDS             8134..8388
                     /locus_tag="ON498_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939030.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            8431..8826
                     /locus_tag="ON498_000734"
     CDS             8431..8826
                     /locus_tag="ON498_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939031.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            8826..9881
                     /locus_tag="ON498_000735"
     CDS             8826..9881
                     /locus_tag="ON498_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCR3939032.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            9878..10084
                     /locus_tag="ON498_000736"
     CDS             9878..10084
                     /locus_tag="ON498_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939033.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            10096..10419
                     /locus_tag="ON498_000737"
     CDS             10096..10419
                     /locus_tag="ON498_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939034.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            10412..10678
                     /locus_tag="ON498_000738"
     CDS             10412..10678
                     /locus_tag="ON498_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939035.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(10841..13186)
                     /locus_tag="ON498_000739"
     CDS             complement(10841..13186)
                     /locus_tag="ON498_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3939036.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            13465..14367
                     /locus_tag="ON498_000740"
     CDS             13465..14367
                     /locus_tag="ON498_000740"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3939037.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            14578..16353
                     /locus_tag="ON498_000741"
     CDS             14578..16353
                     /locus_tag="ON498_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR3939038.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(16663..18249)
                     /locus_tag="ON498_000742"
     CDS             complement(16663..18249)
                     /locus_tag="ON498_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3939039.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            18500..18724
                     /locus_tag="ON498_000743"
     CDS             18500..18724
                     /locus_tag="ON498_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR3939040.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            18866..20617
                     /locus_tag="ON498_000744"
     CDS             18866..20617
                     /locus_tag="ON498_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3939041.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            20617..22401
                     /locus_tag="ON498_000745"
     CDS             20617..22401
                     /locus_tag="ON498_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939042.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            22858..23709
                     /locus_tag="ON498_000746"
     CDS             22858..23709
                     /locus_tag="ON498_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3939043.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            23805..25691
                     /locus_tag="ON498_000747"
     CDS             23805..25691
                     /locus_tag="ON498_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3939044.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(25732..26634)
                     /locus_tag="ON498_000748"
     CDS             complement(25732..26634)
                     /locus_tag="ON498_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3939045.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            26864..28267
                     /locus_tag="ON498_000749"
     CDS             26864..28267
                     /locus_tag="ON498_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3939046.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            28264..29700
                     /locus_tag="ON498_000750"
     CDS             28264..29700
                     /locus_tag="ON498_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3939047.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            29845..30771
                     /gene="trxB"
                     /locus_tag="ON498_000751"
     CDS             29845..30771
                     /gene="trxB"
                     /locus_tag="ON498_000751"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3939048.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            30985..32712
                     /locus_tag="ON498_000752"
     CDS             30985..32712
                     /locus_tag="ON498_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3939049.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            32728..33078
                     /locus_tag="ON498_000753"
     CDS             32728..33078
                     /locus_tag="ON498_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3939050.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(33173..34033)
                     /locus_tag="ON498_000754"
     CDS             complement(33173..34033)
                     /locus_tag="ON498_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR3939051.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            34125..34553
                     /locus_tag="ON498_000755"
     CDS             34125..34553
                     /locus_tag="ON498_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3939052.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            34715..34891
                     /locus_tag="ON498_000756"
     CDS             34715..34891
                     /locus_tag="ON498_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3939053.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            34918..35364
                     /locus_tag="ON498_000757"
     CDS             34918..35364
                     /locus_tag="ON498_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR3939054.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        11591 a         6296 c         7581 g        10009 t
ORIGIN      
        1 gtagagaaca ctctaataga aatgaggtag catgaatgtc agaagttgta aaagttagcc
       61 aattagtaaa aaaattatct ttggaaatcg tcacaggcga cgagaaaagt ttagaccgcg
      121 tcatcgtgac tggggacatc tcacgcccag gactagagtt gaccggttat tttaattatt
      181 attctcataa tcgtttacag ctatttggca gtaaagaaat cagttttgcg gaacggatga
      241 tcccagaaga gcgtttgatc atcatgagaa gattatgtag tgaagatatg cccgcattta
      301 ttatttcaag aggattgccg gctccggaag aaatgatcca agcagccaat gaaaatggga
      361 tagctgtgct gcgttcgcct atctcaacgt ctcggctgct aggtgaatta tctagttatt
      421 tagatgggcg cttagcccct cgaacaagtg tccacggtgt gttagtcgat gtctacggat
      481 taggtgtact aattcaaggg gacagtggga tagggaaaag tgaaacggcg ttggaactga
      541 ttaagcgcgg acatcgattg atcgcagacg atcgagtaga tgtctatcag caagatgaac
      601 tgacggtaat cggagaacct cctaaaatcc tggaacatct gattgagatt cgagggatcg
      661 ggatcataga tgtaatgaat cttttcggtg cgagtgcggt acgaggctct atgcaagtcc
      721 agttagctgt ttatctagaa gcatgggcga aagataagaa atacgaccgt ttgggttctg
      781 aagacacgac cgttgaaatc gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa
      841 caggacgaaa cgttgcaatc atcattgaag tagccgcgat gaatttccgc gcgcgtacaa
      901 tgggctttga cgcaacgaaa acatttgaag aaagattaag tcgcttgatt gaagaaaact
      961 caggcaatga ctagcaagga gtgacaatat gttagcacaa atcaatcgag ttgcttttga
     1021 gcttgtagga attcctatct actggtatgc attgattatc gtatcaggaa tcattattgc
     1081 catgtggctc agcagcagag aagctgttcg ggtaggatta aaagcagatg acgtgacaga
     1141 ttttatgttg tggggattgc cgatttccat tattggtgcc cgtttgtatt atgttttatt
     1201 cgatcttccc caatatatag ctgatccaat ccaaatattc aatatccgtt caggaggatt
     1261 agcgatttac gggggattga ttgcaggtgc aatcacactg tactttttta ccaaatatca
     1321 ttttatttct ttgtggacct ttttagatat cgctgcacca agtgtattac tggcgcaggc
     1381 aatcggacga tggggaaatt tcatgaacca cgaagcgtat ggtccagaca cgacacgaag
     1441 tttcttagaa agtctgcatt taccgcaatt catcattgat aacatgtata tcgaagggat
     1501 ctatcgacaa ccaacctttc tttatgaatc actttggagt ttgattggat ttattctgct
     1561 tttggttttg cggaagaaac cgcatctttt gaaaaaagga gaagtccttt tatgttatct
     1621 aatgtggtat agtttcggac gtttctttat cgaaggatta cgcacagaca gcctttactt
     1681 tttaggtgtg atccgcattt ctcaattatt gtctgctgtt ttacttgtcg gcacattgat
     1741 tctgtttatt tggcgtagaa aaaaaggaaa tttaccggat tatgaccgct cattaggaaa
     1801 aaatcaacaa attatttgat ttttagagcg ttaaaaatga aaaaaatttc aggagtggta
     1861 aaatgaatta tagagtagcg gttttaggac caggctcttg ggggactgct ttagcacaag
     1921 tcctcgctga aaacggacat gacgttcgga tttggggaca tcgtgcttca caagtagaag
     1981 aaatcaacac ccaacacacc aatcaacgtt atttaccaga tcatcatcta cccgattcca
     2041 tccgagcatt caccgatatg gaagatgcag taaaagatgc agatgcggta ctattcgtta
     2101 tcccgacgaa agcgatacgt tctgttgcaa aagaactagt tgccaaattg aatacgaagc
     2161 cagtgatcat ccacgcaagt aagggattag aacagggaac gcataaaaga atctctgaaa
     2221 tcttgttaga ggaaatcccg acagaaaaaa gaaaagctgt tgctgttctt tctgggccaa
     2281 gccatgcgga agaagtagca aagcatgaca ttacgacgat cacagcggca agcacagatg
     2341 caaaagcagc aaaatatgta caagagctgt tcatgaacca ttattttcga atctatacta
     2401 accctgatgt ggttggtgta gagatggggg cagctttgaa aaatattatt gccattggag
     2461 ctggtgctat tcacggctta gggtttggcg ataatgcaaa agcggcaatc atgacgcgcg
     2521 gattagctga gatcagccgt ttaggtgtag caatgggtgc caacccgctg acctttattg
     2581 gcttaagcgg agtaggcgat ctagtcgtta cttgcacaag tgttcattcc agaaactggc
     2641 gcgcaggcaa cttgttagga caaggacagc ctctagaaga ggtgctagcc aatatgggaa
     2701 tggtcgttga aggtgtgaat actactcaag cagcgatgga actagcccaa atgatggaaa
     2761 tcgaaatgcc catcactcaa acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa
     2821 cagccgcaaa agaaatcatg ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa
     2881 ttttttgaag gagcgaatgt atatgaaagt aaaaaaagca gttattccag cagcaggatt
     2941 aggaacaaga tttttaccag caacaaaggc gatggcaaag gaaatgttgc caatcgtgga
     3001 caaaccaacg atccaattca tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt
     3061 gatcgttaca ggaaaagcta aacgaccaat cgaagatcac tttgattcaa atatcgaatt
     3121 agaaacaaac ctaagtgaaa aaggaaaaat cgaattattg aaattagtag aagaaacgac
     3181 agatgtcaac ttgcatttca tccgtcaatc tcatccaaag gggttaggac acgccgttct
     3241 tcaagcaaga gcctttattg gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat
     3301 ggaagacgaa gtaccattga cacagcaatt gatcgatgac tatgaacgta cacacgcttc
     3361 tacgattgcc gttatgcaag ttcctcacga tgaaacttca aaatacggaa ttatcaaccc
     3421 aggggaagta ctggaagatg ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc
     3481 aagcaaagca ccaagtgatt tagctattat tgggcgttac ttattgactc cagaaatctt
     3541 tgatgtatta tcagaacaac aaccaggtgc tggaaatgag atccaactaa ctgacgcaat
     3601 cgatacgttg aataaaacac aacgcgtatt tgcccgtaaa ttcacaggta aacgttatga
     3661 cgttggtgac aaatttggtt ttatgaaaac aagtattgaa tatggtctga cacatccaga
     3721 agtaggggca cctttgcgtg aatacatcat gtctttaggt aaaacattag aagcagaagg
     3781 aagcaagatc gatcataatc caaaaggaat aacagaaatc aaagaagaaa agaaaaaata
     3841 atcatttttt tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt
     3901 ttatttgttt aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt
     3961 ttgatcatgt caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt
     4021 gtcgttttca tcattcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag
     4081 actgtcgcag aagcaaatac gacaattgag atcgtcacaa aagacgtaga tcttttatct
     4141 agacaagtag aaggcttgct ggtcaaaagt aatgagctgc tcttggatat caatggaaaa
     4201 gtcgcaacga tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa
     4261 cttaatcatt ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct
     4321 aaagcgactg ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa
     4381 catactgaaa cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg
     4441 cagtagttgg aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta
     4501 aagaacttcg ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt
     4561 acacagatat ggcaaaagaa aaatcagctg agttaacagg cattgcaaaa gaaaaagcgc
     4621 aagatctttc tcaacaagca ggggatctag caggtaatgt taagcaaaag gtaagtgaat
     4681 cagcagagga agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag
     4741 ctgatttatc taatcgtttc aaaaaaacag cagaagatct gaaaaaacca gcagacgaat
     4801 taggtagtat tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag
     4861 ataaagcagg agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca
     4921 caaaagaaat gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag
     4981 atacaatgac agattcgact accaatcact aagaatactc atttctagaa taaaaaggtg
     5041 agagcggacc agctcccacc ttttttattt tcattactct aaaacaagta attcttttgg
     5101 tgtagtcgtg aatggcacac aaccatcact tgtcacatac acacaatctt cgatacgtac
     5161 ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat
     5221 cactaagtcg tttccttcta caagagaagg gaattcgtgg acggttgtcc caattccgtg
     5281 acctaaacgg tgattgaaat attcgccgta gccatagctg ttgattacgt ttcgtgcgat
     5341 ttgatcaagt tcacctgctg tcacacctgg gcgaactgcc tcttgtgcgg caagctgtgc
     5401 ttcaagagtg atgttgtaga ttttttcttg gaattctgtt ggtttttgat aagcaactgt
     5461 acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata acactagttc
     5521 atttggtgaa accttcgtgc ttcctggtgt accatgaggg cttgcagcat ttgctcctgc
     5581 aaggactaag gtgtcgaaag acatttgtga aacaccttgt ttttttaatt ggtattcgat
     5641 ttctgcgata attgcttgtt cagtcacgcc ttcttttact gttttgaagc caatttcaaa
     5701 ggcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcttcta ccgtcttgat
     5761 cagttgcaga cgctggataa tagaagttag atcagtgtaa gaatctgcac ctgtcaagat
     5821 atcagagata gcatcgaagc gatcgacagt cagagcattc ttttcaatcc ctacattacc
     5881 aggttgccca tagcgtgcgc gaagctcttt tccgatgatc tcccaagggt tttcgctgtc
     5941 taagtagcca cgtacatcgt acgcccagct gctgttttga gcatcttcaa cttccagagc
     6001 aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcgtg
     6061 aggatcactt tcatatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa
     6121 ttctacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg
     6181 gcaacctact ttctaaatta attttgtatt caataattgt accactgatt gattaaaaga
     6241 ccaacgttct taaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg
     6301 caattgttga gattatttga tatcctattt tagaaatgta agacgtattt tcagaaaaca
     6361 tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag
     6421 cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg
     6481 tcaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa
     6541 atgcagtcgc acgtggcttg gcaagtaaga aaacaacaac tgttggggta atcattcctg
     6601 atgtcagcaa tatgttcttt gcctctttag cacgtggtat cgacgatgta gcaacaatgt
     6661 acaaatacaa tatcatctta gcaaactcag atggcaacga tcaaaaagaa gtcaatgtat
     6721 tgaataattt attggctaag caagtggacg gcgtgatctt tatgggtcac catatcacag
     6781 acgaaatccg tggagaattt tcacgttcaa aaacacctgt tgtactagct ggttccattg
     6841 atccagatga acaagtaggt agcgtaaaca ttgattatgt atcagcaaca aaagatgcag
     6901 taaataaatt agcaaaaagc ggcaataaaa aaattgcttt tgtcagcggt gcgctgattg
     6961 atcctatcaa tggtcaaaac cgtctaaaag gctataaaga agcattaagc gaaaacaatc
     7021 taccatatag cgaaggatta atctttgagg cacaatacaa ctttaaagat ggtctgtctc
     7081 tagttgaccg gatccataat agcggtgcaa cagctgtcta tgtttcagac gatgaattag
     7141 ccattggtat cttggatggt ttattagacc gtggcgtaaa agtaccagaa gaattcgaaa
     7201 tcatcacaag caacaattct ttactgacag aagtagctcg tcctcgtttg acaagtatca
     7261 cacaaccatt atacgattta ggagcggtat ccatgcgcct attgacgaaa atgatgaata
     7321 aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcaa
     7381 ctaaataata aaagatcaat catttagagg ttgttccaaa agtggccaac ttctggaaca
     7441 acgtttattc gagaaaaggg gttatgacaa aattttgtca taaccccttt tttttgtgac
     7501 tttaaaaaaa gagaaaatgg cggaaacaaa ccttacgtcc atcgtatacg acacaatctg
     7561 tgcgtagatg ctacattttg ttcactacta tcaaaattac tttaaaatga aaacgtgata
     7621 aaaaagaaag tagaaaagta gaaaagtaac tctaaaaaat ccaaaaaaat ccaggctcga
     7681 ttgcgtactt atcttaaagg agaaacaatc catcatacat aacccgaaag gagacttcta
     7741 tttgcctaag aaaagacgaa agaccatagt catcattttg accattcttg ctataggaat
     7801 cggaggaaaa ttttacatgg acaaaagaga agcacaaaga cagaaagatt tactcgcagt
     7861 agaaaaacaa tccgtcaaag ttctgaaaaa tacctttgca gatatcaggg aagtgaaggt
     7921 agaagaattt aaaaaaaacc ctgttactgg atgttatggt gcacttgtga caatgacaaa
     7981 taatgaaggg aaatctgttt attttgatta ttttttttca aaacaaatga atgaaattag
     8041 cagttatggt gtagaaaatg aagaaataca aaacgaaggg gttactactc ctaaaataag
     8101 agttatttat tctaatggag aggcggagga agtgtgacag taaaagacga agagtataga
     8161 tatataagtg aacatgttta ttggctagat ccaaaacatc ctcaatatag taaaagttac
     8221 aaagaaggcg ccattaaaaa ctttggagaa acaaaattcc aaatcctaaa aatcaaagac
     8281 tcattggatg gcatgcaggc gatggctgca gcgccaatcg tccatagcaa attggagaaa
     8341 aatttcaaaa ataaaaaaat tcctacaaac tttcgcgtac ttaaataatg aaggtatcaa
     8401 tacttacata aaaagaaagg aggcacgttc ttgtctcgga aaataagaaa actcagctta
     8461 gccattttat taatcgtcat attaggaatc ggagggaaag tctatatgga caaaagagaa
     8521 gcacaaagac agaaagattt actcgcagta gaaaaacagt cagtcaaagt tttgaaaaat
     8581 acttttgcgg atatcaagga agtgaagatt gaaaaatctg ctcgaaatga aatgacggga
     8641 gcatatcgta tgtttgtgac aatgataaat aatcacgatg agtctgttta ttttacgttt
     8701 ggattttgga aagaacgaaa tgaattagga gattatggcg tagaaaataa aagtatacaa
     8761 aaagagggga ttacagtaaa taaaatcaga gtaatttttt ctaataataa ggaggaatat
     8821 atttaatgat ggcagacaaa caatacagtg ttattagtga ggctgtttac tggctagatc
     8881 ctaaacacag agactatgat catacttata aagaaaattc tattaaaaaa attgatagtt
     8941 ataattttaa aatcctaaaa atcaaagact cattggacgg catgcaggcg atgggtgtag
     9001 cgccaatcaa gaacggtaaa gtggatcttt ctcaagtagt gattgctttt gcaggaacga
     9061 acttaagtga ttggaaagat ttggaaacgg atgcaaggag tatcatgaat ctctatggga
     9121 atagaccagg agcaagtttt tctgaaaaag caacaacaaa caagatttta tccgttagtg
     9181 gattgaaaac agcggggcaa atcaattcag cgattgcatt tgctaatgaa gtaaaaaagg
     9241 aatatggaga agctgctatt tctactaccg gccattcatt aggtggattc ttagcattat
     9301 atgtagcagc agagaaacaa tggaaaaatg tcggcttaaa cgggccggac ccgtatgatc
     9361 tcttatcacc agaagcaaaa aaatgggtgg aagaaaatcc cggtatgctc accaattacc
     9421 gaaatcacgc cgattttatc ggtaattttg gtggtaatgg cacaggtgct gaagttcgga
     9481 tcagtatgga aacagcattc ttgaatctgc ttgcttccca cagtttaacc tcttggaagt
     9541 ttgatgaaaa tggaaagctg attattcctg aaaatgaaaa taatgttaaa gctcgaaaac
     9601 aacagaaaga aaaccaagcg acccagttgt tttcctatca gttgcaagaa ctgaacaaca
     9661 tacgtaaaaa actacgatta agtggcggcc ggctgacagt taatgaacaa atttacttag
     9721 atgacacaca agctaggtta gtagtggaaa agttatcagc aaaaatgaaa agcgctatgg
     9781 aagaagttat ccgcatcaat caaagaggaa tttttgagct agagaaaaaa tggcatggca
     9841 agaaggcttg cggcaagcac gtgttgtcgc cccttcgttg actgaacagg aaatcataga
     9901 agcattagct agtgttgtcg ccacaaaaga gaacttagtc gattctgcga aagaagtcta
     9961 tctgctgcga atcaataagg caagacgtat gggggaggcc tttgatacat tggtgaagga
    10021 gatccaagac aaaatcaatg aaatcgtgac acgagatcga gaattagcac agcaattcaa
    10081 ttaaaggaga agtagatgaa actagaagaa ttggaaagag attatcgaag tgaacgaaga
    10141 caattagaag ctcaagaaga tgaattgatc cactttaaga atcaaggatt acgattgatg
    10201 gagcaagagg agcagcgact gcatcatgta ttaaagaact tagctttaga taatgagcct
    10261 ctgtttcaag caagacgaag ttattcgaat tatcaggagc attatcaaca agccctacaa
    10321 gtagaacgac gaaaactcta tcagcagcaa gacgaactag aacgttccta tcgtcaaaaa
    10381 atcagacaaa taactatgga gaaatcatct tatgaatgaa tttttaatgc aattactaaa
    10441 agaattagag aaaaatctac atcgatcaaa tgtacgttca gttggtatgg ggatatctca
    10501 gcaaatttgg gcagaaaaac gattgacaga tcaaaaccac caaatgactg cagaagaagc
    10561 tgctcaagaa ttttctaacc gacatgtaca cttgaaagaa tcagtaaaag ggcaatttgc
    10621 agaaaaacta accgaattat tccaaagaca agaaaagaaa ttaaaacagt ttcgttagta
    10681 aagttatata gaaaaatttt gtgaaaatgg cttttcacat ttttacataa atttgtctta
    10741 atatcgtaga tgcaagcttt tgaacaccat ttattcggaa aaagggagct gtgacaagat
    10801 ttttgtcgca gctcccttta attctgatta atctattaga tcaatcatct ttatcgtctg
    10861 attctttggt ctctggattc gctctagcat aagctgaagc ggtgttccag taatctgtat
    10921 agttttcatc agtaccgcca acgccgaatt taaaggcgct tttcttcgga ccattttttg
    10981 cccaaagtga ttccactgtt tttcctggcg tttggataga ttttttattg acagtaactt
    11041 ttccagctgt ttgtcctgtg aacccagata cttttttctt catcacatca gatgataagt
    11101 caaatttctc atcagtgccg aaaatctcag ggtttgcttg gtaagcttga ttgattagat
    11161 tggcaagata attagccgaa cgtatccttg cttggctgtc cattccagta ttgtcatcgt
    11221 gtcctgccca gctactgata gtgatagacg gagtagagac gatcagccat gaatctctgt
    11281 attcgttggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    11341 ttgaaatgac atctttgaat ggtgtagtga ttttttcatt gatcacactg cgcatcatgt
    11401 cattcatgat cgaggccgtt gcttcggaat aaatacggac agaattactt tcatgtttat
    11461 aaatcacatt acctgcatta tctgtgatgg aatcaatgat atagccttct tcgtagacgc
    11521 caccattagc taaggcctgg aatccatttg tctgttgaag cgtcgttaca tctactgtcc
    11581 ctaaaggagc agattcaacg ccccaattat catttgctgg gtagttcatc ttcgccaagt
    11641 agtgctcgta agcatattgt ttcgatccac ccgaattcaa tacgtcttgg tataagtgat
    11701 aagctgggat attgttggac cattccagtg actcacggac ggtttggaag gtgttcgacc
    11761 cttcattggt cgcattgacg attttctcgc cagcattttc tccttcttgc caagtcgttg
    11821 cataatctga tacacgggat tctgatccga caagtcccat atcgattgca ggaccgtaaa
    11881 caagaaccgg tttgatggat gaaccagctt gacgttctgt atcaaaggcg tggttatttt
    11941 gattctggct gtaattccgg ccacccacga atccatagat acgtccagtc ctattgtcca
    12001 tcaatacatt tccagtctcc acttggctac cagcaccatc atctaacata tagccgtaat
    12061 tttctacgcc ggtttgcaat gcagcataga tatctttatc gatcgtcgaa tggatcgtat
    12121 atcctttgtt ttggatcgtt tgctgtgctt tttgataata agcttcactg accgatgaat
    12181 ctgacatatc agcattatct ttttctgcca attgttgtgc aataatcctt gttgcttcgt
    12241 tcatcacggt ataatacaag aattctctgt cagtctgttc cgcgatttgt tgtgggagaa
    12301 agtcttttgt aagatcatag gctttcgctt cttcgtattg ttctttcgta atttgtcctt
    12361 cacggtacat actgaataaa acaaaatctt ttctggcaag accggcagaa agatcttctt
    12421 ttagcgcgcc tgtattcgtg tagggactat acgtgatagg gctttgaggc aaaccagcga
    12481 tataagcagc ttgtggcaga gtcacatctt ttgcatctac accaaagatt cctcttgcag
    12541 cttcctggac tccagcaata ttttgcccct tattattccg accaaatggc gagacattca
    12601 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaacga
    12661 tctcgtttgc tttacgtttg aatgttgttt catcggttag gatctgctgt ttgactaatt
    12721 gctgtgtcaa agtggatcct ccagacgagc cgatgccagt agcttctgaa actaatgctc
    12781 gtaaaacagc tttagggaca taaccttggt gtttatcgaa gtattcatct tctgtactga
    12841 taatcgcggt tttcaataaa ggcgaaatct tatcagatga aatagtcgtc cgcatcagat
    12901 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttccgtaa
    12961 tgtttcctag ttctgtctgc agttcttttt tagtcggcaa ttgtgtgtct tcgacaagat
    13021 aggcaaagta gccagcacct attccgatac ctagagctgc gaacaacaaa agaagaacag
    13081 taataaaaac agtcaatgat tgaaacactc taaaaataac cttaggaaca aaccattttt
    13141 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    13201 ctaatggttt tatttcgcta attatagcaa ataataagac aaaaaaacag acaaggctgt
    13261 ttttgacagg aatttctttt tcgaatgaga taaaacgtga ggaaatgatc ttttttatga
    13321 aagagttctt aagcttttct ttagagaaag tttcagttac tatggagaac agggcaaaga
    13381 aaaacaaacc attttctttg tacatcatct taccaatata taggaattgt tcgaaacaat
    13441 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    13501 aaaatgctgt taggcgcggc agaagaagca gccagttacg gatcttcgac ctttatgatc
    13561 tatacaggcg ctcctcaaaa tactagaaga aaagctatcg aagagatgaa tattgatgcc
    13621 ggaaaagcat ttatggcaga aaacggacta acaaatatcg tcgttcatgc gccctatatc
    13681 attaatttgg gaaatacgat caaaccagaa atgtttccat ttgctgtaca atttctgaga
    13741 gaagagattg ttcgtgcgga agctttaggt gctactcaaa tcacccttca cccaggcgct
    13801 catgtaggag caggggcaga aggtgggatt gctagaatta ttgagggttt gaatgaagtt
    13861 ttgacaaaag aacagacggc acagatcgct ttggagacga tggcagggaa aggtacggaa
    13921 atcggtcgaa catttgagga actagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa
    13981 ttatccgtta cttttgatac atgtcatacg aatgatgccg gatataatat aaaagaagat
    14041 ttctcaggtg tgatggaaga gtttgataag gtcatcggat tagatcggct taaagtactc
    14101 catatcaacg attctaaaaa tccacaaggt tctcataaag atcggcatgc caatatcggt
    14161 tttggaacaa ttggatttga cacattgaac aaaatcgtcc atgatccaat gtttgacaac
    14221 atttcgaaaa tcttagaaac tccatatgtc ggtaaagata aaaagaaagc ttacgcacca
    14281 tatggaaaag aaattgccat gttcaaagcg caagcattcg atcccgaagt gtttcctgag
    14341 ttattagcag aagaagtcac actttaagtt ttctatctga cagcaacaaa aaaggattgg
    14401 gcagtctgcc ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtcgttttc
    14461 gccatgctcc tttcgccccc tctttttaaa ttcagacaaa cagggtatac tgatagaaaa
    14521 gtaaaaaacg aaaagattca caagcaagta gcaaaacgtg attgaaagga cgaaaagatg
    14581 ctgaacactt taaaaccctt gatcaaccaa ctaaaaaaga aacatacata ctttattctt
    14641 ggcatagccg tattatttac aattattcgg ttgtatttat atagtaaagc aatctatgga
    14701 acagatatgt cggcaaccta tgatgatcag ctgctgattc actatgctca aaatattttg
    14761 gatggtaaat ggctagggga gtacacgact acgacacttt caaaagggat ctcctacagc
    14821 ttgtttttag tacttgcaca taaactgcat ctttcttatg cagttttgtt cggcatgttc
    14881 agtattcttg ccagcatatt ggttgcacta gcgtttaaac cgatcgtgaa gaacaagtat
    14941 cttcttttaa gtatctattt ttattttttg ttttctccga ttagttttac acatgagtat
    15001 tccactcgaa cgtatcgtaa tgctctcgtg atcccggctg ttgagatcat tatcgcttgt
    15061 ttactagcta tttattaccg taaatatagt tcccgcagaa aattgctgcc gtggttttta
    15121 gggttagctg tttcttttcc tttcttttgg ttcattcgcg aagattccat ctggcttctt
    15181 ccttttactg tagtcgcgtt aggcatcagt ttcgttcaga tcttgctagg acaagacttt
    15241 tctttcaaag aagcaaagaa gttaaaagaa cgaccatttc tcctttcgaa acaagaatgg
    15301 ctgaaacttt cattgttttt actccctcta gtcggtttct ccatcgtcta tcagtggatc
    15361 gaatataaaa acgagacaac atatggtatt gccaccatca atgaccgttc gagtggtgaa
    15421 tttggacgtt tgatgaaaca gctgatccga atagatgacg aaacgaactt aaataaagaa
    15481 aacagtgaga tatgggtctc gcatgctgca ttaaataaag cattagacgc atctcctaca
    15541 tttcatactg tttctactcg tattgacgaa ttgtatacga ctcatgcgtg gacccaaggc
    15601 ggaaaagtaa aagaattacc aggagatatc attttttggg cattgcgaga tgtggtaagc
    15661 gaatctggtt attaccaaaa caatgcaaaa gaaaccaatg atttttggaa aaaagtcaat
    15721 caagaattag aagaagccta tcaaaaggga actatcaaaa agaaaaaaga attctatctg
    15781 atgggatcag gggatggaaa gacgatgaat gatctgccaa tcgtatttgc ttttctgaaa
    15841 tcaggatatg cctatgcatg gcgatacaaa gatttccatc aaggagggaa ttatagtttc
    15901 gggacgcaag aacaagtcga acaggcccaa gaattattga atcttccact aatggataat
    15961 tggatcgata gcagtgatcc gagaacacct gatttccagt tgacaaaaac agcaaaagtc
    16021 gcgaatgtca tgataaaaat ttaccaagtt ctggccattc ccgtgctgat tgcttcaata
    16081 gcagggatat tacttatgat actaggaacc gtatttgcaa aagctgatcg tgcctattat
    16141 agttcctctt tgattgtagt caccggatta atactgacac aattggtttt tcttttcggt
    16201 gtctcgtggt tttgttctta tgcgccagaa aaaaaggatt attttttaag tgtctacact
    16261 ggcgcaggcg tgccattaac acaattcatc gtagtaacaa gcatgctagt tttcagtaaa
    16321 ttgaatctga aaaaagcttt atcttctaaa taaaaaggga actgtgacaa aagtcttgtc
    16381 gcagttcctt tttccgaata aacggtgttc aaaagcttgc atctgcggta ataagctcaa
    16441 ccaaacgtaa aatatgagga actatttttg tttggctgtt cttattactt gatgcaggac
    16501 agctttttca caacctctga tacacggtgt tccagaagta acttcttcga aaaataagcc
    16561 gaaatttcac aaaaatttga aaagcaattt tcttaaattc cttcttattt cttgaagctg
    16621 cccacttctg tcacaacctc ctttgtgtaa aagaatcagt ttctattcga tttcttctat
    16681 cgtttcttgc aaacgctgtt catatttttt gtttccttta tataaatcac caatcgtcca
    16741 tgccaacagg agaaggacaa gaacaatgat tacataagcg atttgatcag ccaatgctag
    16801 ttgagctgag gtggcttgct cgccgaagaa actttcgatt tgacctggta atcctcgtaa
    16861 gttcaagtaa gtcagactaa tcacagagaa ccagccaagt agttttacaa caaatgaatt
    16921 tttgaatcgg ctccccatct ccgcttggct attggtcatc atcagtaaag gaatcatcga
    16981 gaatggcaat gcaaaagcca gaaaaacttg ggattcattc atcaagttat tcaaagcggt
    17041 atgttcttcg atcgttcctt ttgtacttgt aaacaaaaca caaatcaata caggaatcac
    17101 agaaagcaat cgtgtgacta gtcttcttag ccaaatcggg atacgcatat ggatgaagcc
    17161 ttccatgatg acttgaccgg tcaacgtacc agtaatagta gagttctggc cagaagcaag
    17221 aagtgctaca gcaaataaag tggacaagat accagatttt gcgacagcaa tcaagatacc
    17281 attgctcata gctgatggat cagataaagc ttcatacaga ccaaaaaagg aaggatcttg
    17341 gacagaaccg gttttgaata cagcgacacc catgatcaat agcagtgcat tgactagaaa
    17401 agccattgac aactggatat tagaatccca agtagaaaag cgtaccgctt gtgctacatc
    17461 ttcttcatct tggtgatcga tcttccgagt ttgagaaact gctgaatgaa gatataagtt
    17521 atgcggcatg actgttgcac caataatccc taaagctcca gtcagcggag tttcaccgcc
    17581 gatgaccggt gagtctgaga atgtatgact gttagggata aagccggcaa acaaactgtg
    17641 ccaatcaggg ttagacaagc caacttgata gacgaaaacg aataaaatga cgaaaatcaa
    17701 gcagacaacc aaagcttcga ttttacgaaa cccgactttt gtcagcaata agaggacgaa
    17761 aacatctaac acagtgataa atacggcaat cactaatgga atatcaaaaa gcaggtagag
    17821 ggcgattgct gcaccaatga cttctgcgat gtctgttgcc ataatggcta attcagtcaa
    17881 gatccataaa atgacaccca atgtcttact tgtccgtgct cggattgctt gggccaaatc
    17941 catttgggtc acgattccta attttgctgc catatattgt aaaagcatag caatcagact
    18001 agacatcaaa ataactgaca tcaataaata ttggaaattt tgtccaccag tgatcgaagt
    18061 ggaccagtta cctggatcca tataacccac tgctactaat gcacctggtc cagagtaagc
    18121 aagaagtgtt ttaaaaaaac tttttccctt gggtacatcc actgttccat ttacttcagc
    18181 taaagataaa ccatttgtat gttcaataag tcgatgctta tgaaattgtt tgttggattg
    18241 attattcaag aaaaaaccac ctttcctatt tatctacgat tacattctac acctcagaaa
    18301 gaaaaaatta aagaaaaata ttcggtatgc ctaatttttt ttcgtggcgt gtcttgctgg
    18361 ttaatgaaag aaattttgtt cttctctaaa aaaaccacta ttttttgcat gaaactaaag
    18421 aatacgcctt tcttttttca ataaacttta gtaaaataaa tcattgagag caattcggaa
    18481 atagaaagga gagatttata tggtatcaac aggtattgtc gtacttattg ctattattgc
    18541 tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga aagactattt
    18601 agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt caatggggca
    18661 aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc aagggaaaaa
    18721 ataggttctg acccaaagac tttttgcggg ctgatttttt cagtccgttt tttatttcgt
    18781 aatttgttag actattcaga ctattcagca aaaaagacaa gcacagacaa tcctatgctc
    18841 ttgataaaga caaaggagga gtaaaatgtc gatttttaga aaattaggtt ggttttttcg
    18901 acaggaaaag aaacattatt taattgggat tttctcccta ttcgcagtgg ccttggtaca
    18961 gttagttcct ccaaaggtga ttgggatcat cattgatgag attgccgata aaaatttatc
    19021 aataaagatc atttcctggt ggatcttatt attgatcctt gcagctctct tacaatatct
    19081 ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcggttgg agaaagaact
    19141 tcggcagcag ctattcaaac attttacaga aatggacaat ctcttttacc aaaaatatcg
    19201 gactggagat ttgatggctc atgcaacgaa tgatttgaat gcaatccaaa atgtagcagg
    19261 agctggtatc ttgacctttg cagattcagt gatcacgggc ggaacaacga tcattgcgat
    19321 gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc ctttactagc
    19381 cgttacttca cgagtgctgg ggtcaaaact ccacgatgct tttcgtgatt cgcaagaagc
    19441 tttttctagt atcaatgata agacacaaga aagtattact ggaatcaaag tcattaaaac
    19501 atttggacaa gaaaaagaag atctcgcgga ttttgaagaa aagattgaag atgcaattat
    19561 taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta ccttgattat
    19621 cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg aaggcacgat
    19681 ctcgatcggg cagatggtct catttattag ctatatcggt atgcttgttt ggccgatgtt
    19741 tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg atcgcgtcaa
    19801 tgaacttttg catgaaaaga cgcatatcgt ggaacaactg ggtgcagtgc agaccccggc
    19861 aaaaggcgag ctgaatgtag ctatttcctc ttttaactat ccgaaagaag aatcaccagc
    19921 attagaacaa atcaaatttg tgatccaaga aggagaaaca ttaggtattg ttgggaaaac
    19981 tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg attatcaagg
    20041 aaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt tattaggatc
    20101 aatcggctat gtgcctcaag atcacttctt gttttcgatg accattcgag ataatatccg
    20161 tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt tagcatttat
    20221 caacaatgaa atcaaggaat tgccaaaagg ttatgatacc ttagttggag aacgtggagt
    20281 ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga tcaaaaatcc
    20341 tgaattgctg atcttagatg atgcactttc tgcagtagat gccaaaacag aagaagctat
    20401 cttgcataat ttaaaagaaa taagaaaaga gaaaacaacc atcattgcag ctcaccgttt
    20461 aagcagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa tcattgaacg
    20521 aggaacccat catgagctgc ttgcattagg cggctggtat gaacgaatgt gggagaaaca
    20581 gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta tcaatcggaa
    20641 tggacaaaat cgattccact taaagaacag aagaagatca tcaaacgact gatgaagttc
    20701 gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc tctgtctgtg
    20761 atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct ggctaaacag
    20821 tcggctacga cacaagttat tttgtttttt gctggtatgt acttgtttgg agtcatagtc
    20881 aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcttcatt gaagacctat
    20941 cagtatgttc gggtaagatt atttgaaaag ctgcatacac taggtatgcg ttattttgat
    21001 cagacaccgg caggatcgac cgtttctcga gtgacaaatg acacagaaac gctatttgaa
    21061 ttttggtatg tctttttgat ggtattgact gggatctttg cagtcgtttc ttccttcatt
    21121 gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttctt gccgatttta
    21181 ggaatcgtca tctggtatta ccagaaattc agttctcgta tttaccggaa tatgcgggaa
    21241 aaattaagtc agctgaacac gaaattgaat gaatcgattt ccggcatgcg tattatccag
    21301 cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa cgagtattta
    21361 gacacgcgct atgcgatgat caaaacgaat tcgattctgt taagtccgat catcaatttt
    21421 ctatatgcat tggcgattgc gttggcgttg actttgtttg ggatcgatgc gctgcattct
    21481 cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt ttttaatcca
    21541 atgtctcaaa tgatggactt tttgagcacc tttacagatg gaatggttgc cggtagtcgt
    21601 attttgaaaa tcatggatac agaagaatat gcaccgcaac aaaatcctgg agcagatcaa
    21661 acgattactt caggtaagat cgagtttcgc aatgtaagct tttcttatga tggagagcac
    21721 aatgtattga acaatatctc ttttgtcgct aatccgggag agaccgttgc attagtgggg
    21781 cacaccggaa gcggaaaaag ctcgatcatc aacgtattga tgcggtttta cgaattttat
    21841 gaaggccaga tcttgattga tgatcatgac attcgagaat atccaatgga agagttacgt
    21901 aagaaaatgg gattggtctt gcaagatgcc ttcttgtttt atggagatat cgcaggaaat
    21961 atccggatga tgaatccaac tatcacagac gaacagattc gccatgcagc tgagtttgtt
    22021 caagcaaaca agttcatcga tgaattacca aaaggatatc actcaaaagt gatcgaacac
    22081 ggtgccagct attctagcgg acaacgacag ctgatcactt ttgcgcgaac aatcgtgaca
    22141 gatcctaaaa tactagtatt ggatgaagcg acagcaaata tcgatactga aacagaagga
    22201 ttgatccaag aaggattgac aaaaatgcga caaggacgga caacgattgc gattgcccat
    22261 cggctgtcca cgattcggga tgcagaattg attttagttt tagacaaagg tcatatagta
    22321 gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga tatgtacaaa
    22381 ctgcaaagca gtggtgtgta aaaagtggtt gtgacagggc tccgttcagc tttaggtgat
    22441 aagaaaaaaa ttgtaaaaaa ggctttccag atttttgcat aaatttggct tatcaccgat
    22501 aagctgatac tggaacaccg tatattagaa gttgggacag tagcatagta gcattttgac
    22561 ctaagcgcta gagtgaaaat aaaacagagc ttcccagatt cttcatctgt gagcggtagt
    22621 gtcgtagtca ccagatttaa aaaagattcg acagctagtc agaagtgcct aatagccact
    22681 aagaaagcgt tgacaaaagc gcttgctttc gataatataa agaaaacgat acaacttgga
    22741 tagtgattgc ctgatgcaag ctaaacctga tgagtctccc atggttccat ggacggcttt
    22801 ttgggttttt ctttttgttt tggatacagc cctatatatc atggaaagga gtaaaacatg
    22861 ctgattgaga aagtactgaa taacaacgtc gtaataacca gagatgaaag aaatcaggaa
    22921 atgatcgtga tgggaaaagg tttggcattc aaaaagaaag tgggtgaccc gatttttgac
    22981 tcatgcatcg acaaaaaatt tcagttgaca aatgacggac tttcagaaca gttccaagaa
    23041 ttggcacaag cgattcctct tgagtactta gaaatttctt gtgaaatcat tgaatataca
    23101 aaagaggctc tacaagtcca gttgaatgat tcagtgtata ttgcattgac ggatcatttg
    23161 tatacggcta tcgaacgtca caaaatggga atatttgtac caaatgtatt gctgtatgat
    23221 atcaaacaac tatttcctaa agagtatgcc atcggggaaa aaacagtgaa gaaaatctcg
    23281 gataaatacc acatcaagtt atcagaagat gaagccggat ttatcgcatt gcatttagtc
    23341 aatgcacaag gcattcaaag ccaacaagac aatacctacc tgtttactac aacaatacag
    23401 gatattctaa atatcgtccg ctattactat aagattacat ttgatgaaga ttcagtccat
    23461 ttttttcgat tcgtcacaca cctacgtttt tttctgacac gcgtcctaga tggagaaaaa
    23521 tcagaagtgg gagaagtttc tgaggaattg taccaagtta ttcaaaaaaa atataaggat
    23581 acgtttggtt gcgttgagaa aatagcagac tttttagccg agatattcaa ctaccagatg
    23641 tccacggacg aaaaaatgta tttaacgatc catactgcgc gtttagtgca tgagaatcgt
    23701 tgtaaataaa taaaaaagga ggataaatta acaagaacgc atgaaataag ggtagtgaca
    23761 ttcagttggc taaaccttcc actaataaat tggagggaat agaaatggga aaatatcaag
    23821 aattagcgag caaaattgta gaaaatgtcg gcgggaagga aaatatcaat ggtttgacac
    23881 attgtatcac aaggcttcgt ttcaaattaa aaaacgaaga aaaagcaaat gatgagatct
    23941 tgaaaaatat ggacggtgtt gtgacggtaa tgcgagcggg aggacagtac caagttgtca
    24001 tcgggaatca tgtgccagtc gtgtttgaag aggttataag agcaggaaac cttacttttg
    24061 atgaggctgt ttcaacagaa aaaatgagac cgtttgatat gcttattgat ggaatatcgg
    24121 gctgtttcca gccattcctt gctattctag ctgctggtgg gatgattcgt ggactgacag
    24181 cattacttgt cttcttaggc gcatttgacc gcggttctgg cacatttgtt atgtttgata
    24241 atatcggtga ttctgtattc caatttatgc ctgttattat cggtttaaca gctgcccgta
    24301 aatttaaagt aaatgaattc gtagggatgt tgcttggggc agcattaatg aatcctagct
    24361 tgtcgctgga agctttgagt ggtgcagcag aagcaccact gacgaccata ttttctggca
    24421 ctatttttga agcacctatt tatcaaacag tattcggcat tccctggatt gcgcgtaact
    24481 acgcttcttc tgtcattccc attatcttta ttgtgttgtt agctagccaa attcagaaac
    24541 caattaaaaa gttagtacct gagatgatcg cgaatttctt cgttccattc ttcaccgttc
    24601 ttattacaat gcccttaggt tttttactcg ttggaccagc attcacattc gcaactgata
    24661 ttttaatggc tggatttgaa acacttttag cactttcgcc agttatttat ggagcaatcg
    24721 taggcttctt ctggcaaatt ctaatgatgt ttggtctcca ttgggcaatc gtaccaatgg
    24781 gattgatgca attttctgtg aatggctggc aaaatatcat gacacctgtt gcagtagtgt
    24841 cttttggaca aactgcagca cttactgcac tctactttaa gcttagaaat ccaaaagaca
    24901 aagcaattgc aataccagcc attgtttctg gtattgtggg gattacagag ccagctattt
    24961 atgggtttac attgccacgt aaaaaaatat ttatttttac ctgtgtcggt ggtgcgttag
    25021 ggggcgcgta tagtgggtta atgaatttaa cttcttggaa tcaaggtgga ctaggcatct
    25081 ttacaattcc caactatatt agaccagatg gtaatttaac agatgtgatt aatgtattga
    25141 ttgggatcgc cattgcgatg gtggtatcct ttgcgctgac ttttttcttt tggaaggagg
    25201 aagcagatga aacagatgat agacagaaaa catcaggtaa agaaattgta aaaacaccga
    25261 tacaaggaca aatcgctcca ctgaatgcag caaaggatgc agcttttgca caaggaacat
    25321 taggtcgagg aatcttgatc taccctgaaa aaggagaagt acgtgcccct tttgacggaa
    25381 cagtcatgac cctgttcccg acgaaacatg caatcggaat ggtttctgaa gcaggtctgg
    25441 aattgctgat tcatgtcgga ttggatactg ttcagttaga aggaaaatat tttaaaagct
    25501 tggtgcagca aggagaaaga gtgaaacaag gcgatctgtt gctccgcttc gacaagaaga
    25561 aaatcgaaga ggaaggatac agtttagaaa cacctgtgat cgtcaccaat tacacggatt
    25621 atttggatat tttggaaaat caaggggaga cggtcgggcc agacgatgca cttatcaccg
    25681 cactgacctg aacggttagg tgaaagaatg gactaggctt aattattctt tttagtccac
    25741 ttctttattt tcaaaattga aaggctcttt catgatggct gagctgatag ggcgtttatc
    25801 aaatttttgt cctacagaaa ttagatggac taaattctgt tgataatttt ctgcaaaaac
    25861 acaactatat aacacgtcta gaagataaca aatcgatgta ttgatcgtaa aattaccaat
    25921 tttcgaatac agtcgttcgc gtgtcgtaag tcgcaaagcg gctgtactca ttgaagatag
    25981 cgttgaatca ccgatactgg tcaaagctaa aattggcaca tgattttgtt tcaatatttc
    26041 tgcggtacga aggatcacat tgttttcacc cgtataagaa atcagaatcc cgcacatatc
    26101 actcgtgcaa ttgtacgctt catagaacga ttcaccaaaa gtggtgacaa tcgtgacatc
    26161 cattttgatg cgcttcattt tcagcatgaa atcttgagaa atcaaagtat tcgagttact
    26221 ggcgaaaatc acgatttttt ttgcttgaag cattagttgt ttggcttttt gcagttggtc
    26281 atgatgaatg agagaaagtg tatcttgaat cgtcatctgt tctaaagaag ccattttatt
    26341 tgcgatcgtc ataataccgt ctgtggtgga aaaaggaaga ttcgcatcta cgtcttgaaa
    26401 attcccttca agataggctg tttcagctaa aaaagcatta cgaaaactga cccagccatc
    26461 aaaacccatt ttttttgcta cacggatgag tgtggacggg tggacataac atcgctcagc
    26521 aatctcttgg atggtcattt cgccaagtgc tggagaatcg cttaagagaa actgaatcac
    26581 ttcattttct gaaggagaaa aggagatttc tttgattttt tcacgtaata acatgtagat
    26641 accttcttct tgttttttta cgtaaattat aacttttttg aaaatgattt gtaaacggat
    26701 ttatgttata tagcttaata aagacatttt ttgacaagat atttacaaaa atccagaaga
    26761 aaacactaac ttttgtaaat gttactggaa agaaacgaat ttctattgtt tgttttgttt
    26821 acaaaaaaga taataaaagt gtaaaaagta aaggagtgag aacatggcaa aaggaatcaa
    26881 aatcgctacg attggtggag gcagcagtta cacccccgag ttgatggaag gatttatcaa
    26941 aagatatgag gaattaccga tacgagaaat ctggctcgta gatgtagaag aaggaaaaga
    27001 aaagcttgag atcgtcagtc agatgtctca gcgtatgtgg gatgcttctc cgtatgaagt
    27061 caaaatctat gcaactctgg atcgggaaga agcattgaaa gatgcggatt ttgttacgac
    27121 tcagtttcgt gtcggtcaat tagatgcacg aatcaaagat gagcggatcc ctagttatta
    27181 tgggatgatt gggcaagaaa caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt
    27241 tccagtggtt ttggcaatcg ttgaagacat gaagcgttta tgtccaaatg cttggctgat
    27301 caatttcgcg aacccaagcg ggatggtgac agaagcagtt atcagatatg gcaaatggga
    27361 taaagtgatc ggattatgca atgtgccagt aatggcgatg atgacagaac caacaatgct
    27421 tggcaagaaa aaagaagagc tgatctacaa gtttgccggt ctaaatcact tccattggca
    27481 caaagtagca gataataaag gaaatgaccg cacggcagaa ttgattgatc gtttatacga
    27541 tgaagacaat ggattgccga aaaatatctt tgatatcccg tttttcaaag aacaattaaa
    27601 acaaatgaac atgattcctt gtggctacca ccgctattac taccgtgaag aagaaatgct
    27661 ggaacatgct ttagaagaat atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac
    27721 agaagcagag ctgtttgaat tatacaaaga tccagaattg gatcataaac ctgaacaatt
    27781 gcaaaaacga ggtggtgctt attattcaga tgctgcctgt gaaacgattg cttcgatcta
    27841 tggaaataaa gaaacacaga tcgtggtatc tacaaagaat gatggagctg ttcctgatct
    27901 gccggcagat tgtgtagtag aagtcactgc ctatgttggc gcacaaggcg cacgaaatgt
    27961 agcgtttggt tcccttcctg ctgctgaaag aggctggttg caagtaatga agaatatgga
    28021 attgctgacg atcgaagcag ccgtgactgg tgattatgga acagctttgc aagcgtttac
    28081 gatcaatccg cttatacgat caggaaagac agcaaaacgg ataatggatg agttgtttat
    28141 tgcacatcag cagtatttac cgcaattcaa ggaaacaatc agccggttaa aagcagaagg
    28201 aatcgaagtc caagatgaac tggcaagaga attatcaagc aatacacatg taatggagga
    28261 agtatgatgg gattcagaaa agatttttta tggggcggag ctacagcagc caatcaatgc
    28321 gagggcggtt atgatgaagg cggacgtggg ttagcgaacg tagacgttgt gccaatcgga
    28381 gaagaccgtt ttcctgtcat tactggaaaa aagaaaatgt ttgaatttga tgaaaatcat
    28441 ttttatccag caaaaattgc gatcgatatg tatcatcatt ataaggaaga tattgcttta
    28501 tttgcagaaa tggggttcaa gacttatcgg atgtccattg cttggagtcg tatttttcca
    28561 atgggcgatg aaaaagagcc aaatgaatct gggttgaaat tttatgagga tgtctttaaa
    28621 gaatgtcaaa agtacgggat cgagccgctt gtgacgatta cacattttga ttgtccgatg
    28681 catttgatca aagaatatgg tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat
    28741 ctgtgccgag tcatcttcaa tcgctataaa ggacttgtca aatattggtt gacattcaat
    28801 gagatcaata tgatccttca tgcaccattc atgggcgcag ggctgtattt tgaagaagga
    28861 gaaaatgaag aacaaatcaa atatcaagcg gcacatcatg agttggttgc aagtgccatc
    28921 gcaacgaaga ttgctcatga agtagatccg gagaacaaag taggctgtat gctggcagca
    28981 ggagcatatt atccatacac ttgcaatcct gaagatgttt gggcttctag aaaatcagat
    29041 cgagaaaatt atttcttcat tgatgtccag tcacgaggag aatatcccgc ttatgcattg
    29101 aaagaaatgg aacgaaatgg cattcagatc gaaatggaac ctggggatgc cgcattgctg
    29161 aaagaacata ctgttgattt tatctccttt tcttattatt cttctcgagt ttcgactact
    29221 gacaacgaac tcttggaaca gactgcagga aatatctttg cttctgtcaa aaatccatat
    29281 ctggaagcaa gtgaatgggg ttggcaaatt gatccattag gcttgagaat aacgatgaat
    29341 gatatttatg atcgttatca aaaaccacta tttattgtag aaaacggctt aggtgcagtc
    29401 gatacgcccg atgagaatgg ttatgtggaa gatgattatc gaatcgacta tctggcagca
    29461 catatccaag caatgaaaga tgcagtagaa ttagatggag tcgatctatt aggctatacg
    29521 acttggggat gtattgattt agtatcagct ggtacaggtg agatgaagaa aaggtacgga
    29581 ttcatttatg tcgatcgtga caacgaggga aatggcacat tagcccgttc gaagaagaaa
    29641 tcattcaact ggtataaaaa agtaattgat tcaaatggag aaaatctttc gaatgattga
    29701 tcaatgattt ataaaagtct attgtttttc agcggatttt tcgagtataa taaagctatt
    29761 cgagaaacgc aagcagaaca atagactttt tttcgcattg gctgtaaagt atgcttactt
    29821 gattaagtaa aggagaatct acatatgtat gatgtaatcg ttattggcgc aggtcctgct
    29881 gggatgactg ctgctttata tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa
    29941 ggcgcccctg gcggccaaat gaacaatacg gcagaaatcg aaaactatcc tggtttcgat
    30001 tcaatcatgg gtcctgatct agcttataag atgtatgaag gtgtttcaag atttgggaca
    30061 gaaaatgctt atggaatcgt gcaagatatc aaggatcacg gcagccacaa agaagttatt
    30121 tgtgaagata aaacttacga aggaaaaaca gtcattatcg caacaggctg tatccaccgt
    30181 aaattaggcg tacctggcga ggaagaattt gctggacgtg gcgtttctta ctgtgcagtt
    30241 tgtgatggtg cgtttttccg taacaaacgt ctagtagtga tcggaggagg agactcagca
    30301 gtagaagaag ccatctattt gacacaattt gcttctgaag tagtcatcgt ccaccgtaga
    30361 gatgaattac gtgcacaaaa gatcatccaa gaccgtgcat ttgcgaatga aaaaatttcc
    30421 tttatgtggg atacagtcgt tgacgaaatc gttgggaatg aaatggtagt gacaggtgtg
    30481 aaagcacgta atgtcaaaac cggcaatgag tttgatttgg cagcagacgg agtattcatc
    30541 tatgttggac tggatccatt aactgcacca tttagagaag cagggttgac gaacgaagct
    30601 ggctggattc caacagacaa cgaaatgaag acgaatatac caggcgtttt tgccatcgga
    30661 gatgttcgtg aaaaagattt gcgacaaatc actactgctg ttggtgatgg tgggatcgct
    30721 ggacaacaag tgtataaata cttggaatct ttagaagaaa caactgcata agtttaaaaa
    30781 gagtggaaga acggtttcaa ttgttctttt actctttttt ttcgcgtgaa atctactgaa
    30841 tccgatttat gcgaatactt gcaaccgtgc ttgataaaag aatcctttct tgatttaagc
    30901 gttttttggt cctcatactt ttatatctaa taagtgaatg ctatacttat taagaaccat
    30961 atttcagaaa gaggtgttat tttcatgtct tgggaacaag tgtatgaaca atgggtacat
    31021 gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg atctgggtga agatcctgaa
    31081 aaacttgaag atgcttttta tgcaccgttg gaattcggta ctgcaggcat gcggggaatc
    31141 ttagggcctg ggatcaatcg aatgaacatc tatacggtaa gacaagcaac agaaggactt
    31201 gcacgtttca tggatacaca aggcgaagaa acaaaacgcc gaggtgtcgc tatcgcttat
    31261 gactcacgcc atcaatcacc agaatttgcc atggaagcag ccaaaacatt agcacatcac
    31321 aatatcccat cttatgtatt cgaaagtcta cgtccaacac cagagctttc ttttgcagtc
    31381 cgtcatttga agacgtttgc gggaatcatg gtaactgcat cccacaatcc agcagcttac
    31441 aacggatata aagtctacgg cgaagatggg ggacaaatgc ctcctgcaga cgcagatgca
    31501 ttgacgaaat atgtccgtga agtcagcaat ccgttaaaag ttgaagtatt atctgacgaa
    31561 gaagctgaac atagcggatt gatcacgatc atcggagaag aagtggatgc cgcttatcta
    31621 gaagaaatca aagcagtaac gattgatcat gaattggtgg aaacaatggg gaaagatttg
    31681 aaacttgtgt acacaccact tcacggaaca ggaaaaatgt tgggcgaacg tgcgttgaaa
    31741 caagctggat tcgaacaatt cgtactagtt ccagaacaag ctgtcgcaga cccagatttc
    31801 actacagtca aatcaccaaa tccagaagaa cactctgctt ttgagtatgc gatacgactt
    31861 ggcgagaaag aaggcgctga tttgctgatt gcaacagatc ctgatgcaga ccgtttaggt
    31921 gcagccgttc gtttgccaaa tggctcctat gaagtattga ccggaaacca aatcggagca
    31981 ctgtttgctc aatatatttt ggaagcacac caatcgcgag gcactttacc tgaaaacgca
    32041 gcggtattga aatcgatcgt ttctagcgaa ttacctacag caattgcgga acactatggg
    32101 gctaccatgt tcaatgtatt gacaggattc aaattcattg ctgaaaaaat tcaacaattt
    32161 gaagaagatc attcccatac ttttatgttc ggattcgaag aaagttacgg ttatttagtt
    32221 aaacctttcg ttcgcgacaa agacgcgatc caagcattgc tcttattagc agaagtagca
    32281 gcttactata aaaaacaagg caaaacgttg tatgacggct tgcaaacgat ttttgaaacc
    32341 tatggctact acgcagaaaa aacgatttct gtgacaatga gcggacaaga aggttctgcg
    32401 aaaatctctg ctttgatgga aaaattccgt gaagaagcac caacagaatt tgccggagca
    32461 aaagtcatcc aaacagaaga cttcaaagag ttgacgagaa cagaccgtga aggaaacgta
    32521 gaaaaactaa cgacaccgcc atctgatgta ttgaagtata cgctggaaga aaatggctgg
    32581 atcgctatcc gtccttcagg aactgagccg aaaatcaaat tttacatcgg tgtaaaagga
    32641 aactcttcag aagacgcagc tgaaaaagta gccgcttatg aagcgattat ccaaaagatg
    32701 acagaagaat aaaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct
    32761 tttttcaagt tggaggtgaa tggattgcca gacacgcaaa aaacaaaagt agagaaagtc
    32821 agccaactat tcaaaatgtt gagtgacccc actcgtctca aaattctgct gtatctgaaa
    32881 gacggggaac aaaatgtgac agcaatcacc caagcagtgg aaatggaaca atctgctgta
    32941 tcgcatcaac tgcgtttatt aagagaaaac catgtcgtca agtcccatcg tgagggaaaa
    33001 gcgattttgt atagtcttga tgattctcac gtactggata ttttgaacca gacactcaaa
    33061 catgtggaac atcaataacg ataggtgcaa atagagacaa actggactgt gtcaacgaat
    33121 cttcttcgct gacacagtcc agtttttttt aagaaaaaga caagaaaaac tgttagtctg
    33181 tttcttcggt ggtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa
    33241 taagcgaagc tgtttcttca atagataaag aagcgacatt gattactacg cagcccagtt
    33301 tttcataaag atcatttgca aaatcgagtt cagctcgtat tttttcgata tcagaataag
    33361 cagtatcagg atttaatccg taagagcgca tccgttcttt acggatattg tttaaaatat
    33421 ctgggttatt cgtcaatccc acgatttttt taggatcaat ctcccaaagc tgtttgggaa
    33481 tatgtgcttg tgggatcaaa ggaagattgg caacttttaa atttttgtta gctaaaaaca
    33541 agctcaaagg cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctagaa
    33601 acccacgagg atctttcccg tcatcgtatt ttacagcaaa ttccattgct ttaatccgtt
    33661 tgaaatagtt ctcattcaat aaatgaaggg cgccaggttg ccgaatgggc ttgataccag
    33721 ttaagcgctc gatttctgat atcggcggag tcatcacatc aaaactaaat aattgatgtt
    33781 cttcacaaaa agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa
    33841 gtgctttttc ttctaatgca tcttgcaatg cctgctttaa tgttttttca tctgtcacga
    33901 acgttctgcg gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta
    33961 gttttgtcgc agtttctcca gcagagtcgg agatgacaaa aaaagtaacg atttctgtag
    34021 aatcggaaga catggataaa acctcacttg catttttttt atgtttatta taccatactc
    34081 tagaatgcgt acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat
    34141 tgatcgaaca atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag
    34201 aagcattgtt aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg
    34261 actatatgaa tcaggaattc aaaggggtaa gttatgatac gatttatcgg aatctccatg
    34321 attttgcaga gatcgattta ctggaagaaa ccgatttaaa tggagaaatg aagtttcgtt
    34381 ttcattgctg tcagggggaa atcggccatc accatcattt tatctgtaca gtgtgcggaa
    34441 caacgaaaga aatccatatg tgtccgatga attatttcga agaacagcta ggtggatgca
    34501 cgatcgaagg gcatagattc gagattttcg gaagatgtga aaattgtcaa taagggttta
    34561 aataaaataa catttgataa ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc
    34621 attagatata atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac
    34681 ggttactttt taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa
    34741 cgaatcactt gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt
    34801 acaagaatct cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc
    34861 tgaagcagct agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg
    34921 tcactactaa gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa
    34981 tctttacaag tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt
    35041 cacgacttga cagaagaaga agaattaacg gtgttgtctc gcgaaatgaa acagcgacgt
    35101 gattctttgc atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt
    35161 gaaattgtga tagtcgaaaa atacatgccg gaacaacttt ccgatgaaga aattcgacaa
    35221 cttgttcaag aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt
    35281 atgggtgcaa ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt
    35341 gtgaaagaac tattacaatc ttaattttat catttaaact gcggacagtg attgtccgca
    35401 gttttttgtg ttcgtaaaaa aagaactaaa aaacagctag aagaaagaat cttattcttt
    35461 cttctagctg ttttttt
//
LOCUS       DAJSVD010000018        35204 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSVD010000018 DAJSVD010000000
VERSION     DAJSVD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.018"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(594..1985)
                     /locus_tag="ON498_000758"
     CDS             complement(594..1985)
                     /locus_tag="ON498_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR3939055.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="ON498_000759"
     CDS             complement(2198..2584)
                     /locus_tag="ON498_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR3939056.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="ON498_000760"
     CDS             complement(2640..3263)
                     /locus_tag="ON498_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR3939057.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="ON498_000761"
     CDS             complement(3327..3995)
                     /locus_tag="ON498_000761"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3939058.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="ON498_000762"
     CDS             complement(4007..4447)
                     /locus_tag="ON498_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939059.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5177)
                     /locus_tag="ON498_000763"
     CDS             complement(4506..5177)
                     /locus_tag="ON498_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939060.1"
                     /translation="MTNEIVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(5174..6133)
                     /locus_tag="ON498_000764"
     CDS             complement(5174..6133)
                     /locus_tag="ON498_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3939061.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(6354..7457)
                     /locus_tag="ON498_000765"
     CDS             complement(6354..7457)
                     /locus_tag="ON498_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3939062.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(7679..8149)
                     /locus_tag="ON498_000766"
     CDS             complement(7679..8149)
                     /locus_tag="ON498_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR3939063.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            8406..9725
                     /locus_tag="ON498_000767"
     CDS             8406..9725
                     /locus_tag="ON498_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCR3939064.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            10166..11806
                     /locus_tag="ON498_000768"
     CDS             10166..11806
                     /locus_tag="ON498_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR3939065.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11960..13282
                     /locus_tag="ON498_000769"
     CDS             11960..13282
                     /locus_tag="ON498_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3939066.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13344..13775)
                     /locus_tag="ON498_000770"
     CDS             complement(13344..13775)
                     /locus_tag="ON498_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3939067.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13839..14675)
                     /locus_tag="ON498_000771"
     CDS             complement(13839..14675)
                     /locus_tag="ON498_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3939068.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(14865..15167)
                     /locus_tag="ON498_000772"
     CDS             complement(14865..15167)
                     /locus_tag="ON498_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR3939069.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(15193..15624)
                     /gene="ruvX"
                     /locus_tag="ON498_000773"
     CDS             complement(15193..15624)
                     /gene="ruvX"
                     /locus_tag="ON498_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3939070.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15621..15887)
                     /locus_tag="ON498_000774"
     CDS             complement(15621..15887)
                     /locus_tag="ON498_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR3939071.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16141..16866)
                     /locus_tag="ON498_000775"
     CDS             complement(16141..16866)
                     /locus_tag="ON498_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3939072.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17232..17302)
                     /locus_tag="ON498_000776"
     tRNA            complement(17232..17302)
                     /locus_tag="ON498_000776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17268..17270),aa:Gly,seq:ccc)
     gene            17419..18171
                     /locus_tag="ON498_000777"
     CDS             17419..18171
                     /locus_tag="ON498_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3939073.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18187..18666
                     /locus_tag="ON498_000778"
     CDS             18187..18666
                     /locus_tag="ON498_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3939074.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18917..20497)
                     /locus_tag="ON498_000779"
     CDS             complement(18917..20497)
                     /locus_tag="ON498_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3939075.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20490..21233)
                     /locus_tag="ON498_000780"
     CDS             complement(20490..21233)
                     /locus_tag="ON498_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939076.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21409..22185)
                     /locus_tag="ON498_000781"
     CDS             complement(21409..22185)
                     /locus_tag="ON498_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939077.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22433..23269)
                     /locus_tag="ON498_000782"
     CDS             complement(22433..23269)
                     /locus_tag="ON498_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR3939078.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23280..24209)
                     /locus_tag="ON498_000783"
     CDS             complement(23280..24209)
                     /locus_tag="ON498_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3939079.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24401..24754
                     /locus_tag="ON498_000784"
     CDS             24401..24754
                     /locus_tag="ON498_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3939080.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24828..25484)
                     /locus_tag="ON498_000785"
     CDS             complement(24828..25484)
                     /locus_tag="ON498_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3939081.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25710..27644
                     /locus_tag="ON498_000786"
     CDS             25710..27644
                     /locus_tag="ON498_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3939082.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27730..28953)
                     /locus_tag="ON498_000787"
     CDS             complement(27730..28953)
                     /locus_tag="ON498_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3939083.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28947..30845)
                     /locus_tag="ON498_000788"
     CDS             complement(28947..30845)
                     /locus_tag="ON498_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR3939084.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30839..31957)
                     /locus_tag="ON498_000789"
     CDS             complement(30839..31957)
                     /locus_tag="ON498_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3939085.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32040..32873)
                     /locus_tag="ON498_000790"
     CDS             complement(32040..32873)
                     /locus_tag="ON498_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939086.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32866..33783)
                     /locus_tag="ON498_000791"
     CDS             complement(32866..33783)
                     /locus_tag="ON498_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3939087.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33806..33985)
                     /locus_tag="ON498_000792"
     CDS             complement(33806..33985)
                     /locus_tag="ON498_000792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939088.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(34010..35134)
                     /locus_tag="ON498_000793"
     CDS             complement(34010..35134)
                     /locus_tag="ON498_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3939089.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        10674 a         7117 c         5990 g        11423 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaattttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg ttttttactg atcactttta aaaagctttt tagaatcttt
     4801 tgctgacgtc taaaaataga aaaagaattt tcgtcatcta tttgataacg catttgtttt
     4861 tgcagttctg ctgcagtcag atggatcttc ccatcgtttg tatcgatcat taaaccttct
     4921 ggaaacagtt caaccactgc tcgatcccaa gattcaagag aaagctcgat ataatctttt
     4981 actggtaatt ccaattctac cttaaaaaag tgcaaaacag ccgacagccc ttctgcttga
     5041 tagatttctc ctaatgttag cccctctaga tggaactctg gtgaaaaatg cagcaatgtt
     5101 ttattttcct gcaattgaac atatagaaat gtagatcgct caatcagagt atcttttaaa
     5161 acaatttcgt ttgtcaaatt aagttagaca aatcatcttt tgtgacgtaa ctcaaaaata
     5221 cttccattgg tgtccgatag tgtaatgatt ttctaggaat atgatttcgt ttcgatgcca
     5281 ccgattggat aaaggtttca gatacaccat taaaatccat agctttatac aaaccatctt
     5341 tacgtaataa gccatttgaa tgttcattca atcctcgttg agaaggtgtt cctggatccg
     5401 caaaataaat tgaaatatcg ttggtattac taatcttttt ccaatttgag aattcttttc
     5461 cacaatcaaa tgtgatggat ttaaataagt tttttgggac acaacgaaac cattgattca
     5521 gcctattttc aatgtcttta gcctgtctgc cttcgggttt caaggtaatg atgactttag
     5581 atagccgttc aactaatgtg atgaccgcgc ttttatgttt tccacccacg atagtgtcac
     5641 cttccaggtg accaaattca tgatcaaact gggtatagtc tgtctttctg tctcggattg
     5701 accggcgaaa cccttgtttt cctctttttt ctttatgacc gtttggtttt ctttttccct
     5761 tcattggtaa atcgtgagtg tcaaaggttt tatctttaaa cattcgatac agtgtgcgta
     5821 ccgaacaagc aataggaaac tctgctcgac caataaggac atcaggagcc cacccttgaa
     5881 caaccttttt ttgaatgtat tcctgttgct catcaggtaa aacaatcgga tgtcttccgc
     5941 agttacgttt gtttttctta tattgtttat agtagctcat tgcgcttcca ccagatttaa
     6001 gaaaacgata gaccttataa atagtttgtc gagaacgttg taacagattg gctacttttg
     6061 aaacaggttg tgattgattg aaataagctt ctatcattac gagttcagtt ggtgtaagat
     6121 ggttataggt catttgtact cactccttat aattctttcg tcggaactat tttgagtgta
     6181 gcacaaatga ctttttttag ttgtctagct taattttaca atcggcgaag tatgaaaaaa
     6241 tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt gaactataaa
     6301 aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttgt ttcttaaata
     6361 aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt tccagctaat
     6421 tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac tcctgtactt
     6481 cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc tgcacctgat
     6541 gcgattgctt tgaaaacatg ctgtccgcgt cgtacaccgc tgtcaaacac gattggtaca
     6601 cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgctgctgg accaccatct
     6661 agttgacggc caccatgatt agatacccag attccgccag caccagattc taatgaacga
     6721 tagacatctt cttctgactg gacacctttc acatagactg gcaaatcaga atgagcagca
     6781 ataaattcta catcttttgg actaagcttc tgtttggaag atttgtacac agcatccatc
     6841 gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagtgg gaaggtaaac
     6901 ccatttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt caatacgatt
     6961 gcttttgctc cattacgctt ggccacttca agaatatcca aattgatccc atcatctttg
     7021 ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct tatctcttct
     7081 aatgtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc tacacctttt
     7141 gcagaagctt tctccgcctt cacatgagct aagccatgag cagcaactgg tgccatgatg
     7201 attggtgcag tcagcgtttc tttgtcaaaa tgtgtagttg tatcgggtaa ttctacatct
     7261 cttaatacat gaggaataat cagctgatga ttgaatgccc gttcattttc ttgataagta
     7321 aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat cacttgtgaa
     7381 gcggctattt ctaaatcttc catattgata aaatccacta ttccttcatt tgtacctgct
     7441 tgatacgttt tttccatttc aatgccttcc ctctataaaa aattgaatga ataatatgat
     7501 agcgtttaca cactttatat tactcttccg tattgataca ggcaaataat ctgtttacat
     7561 ttcagtcatt ccagttgtta ctactctgaa agaaatgttt agaacacaaa tagataaaaa
     7621 acgatgggat tttgacaaaa gtcgtgtcaa agtccctttt acgaaatact gtggatgcct
     7681 attgaaataa gtgcgggttg tttctttgta tttcaatcgt ttcttgccat tcttcgttta
     7741 attttggatt accgttttct tccttgaacg cataaggtcc caatccaaaa agctcagtta
     7801 gtatttgatt cacgattcca tatagctcgg tgtgatcgga cacatgatta ttgaagatca
     7861 tctcattatc accacgtttg aatctcacat tcgggctctc atgatgaacc tctatgcagt
     7921 tttccccttt ttttaactga cggatttctg aaaacatctc ataaatatct tctccaataa
     7981 aaccttcagc aagcgccaga ctgtccacaa gaccgcctac tgtcaactca gagattatcg
     8041 ctttgttggc accaagcttt aatgtatagc ttgttgcttc tactacgccg catgcattgc
     8101 aaccaccttc tgatagttcg ctttttctga tcaattgaac ttctttcatt ttctgttcct
     8161 cctcttagtt ttctacttat gtatttttta tcacctgacg taagtgtatt ttatcgtcac
     8221 gatcacgtga tttattcttc agagattcta cctgaataat tcatactttt aattaaaacc
     8281 atgtgaaact gcctcacggc agcttacaat tactttttca tcttcacccg ttaagtgatg
     8341 aaaaaagaac ccctttctgc tatggttaaa gtgactaaac caaccaaaag aaaggagttc
     8401 ttttcatgac tagcttacac aaaaaccaag taaaattcaa ttcaaatatc actatttctc
     8461 atacaggtgg tcaattatcg agtgattcgg gtctcgtcct agtgaaagag ctgatgaaca
     8521 tctttggttt ttctgaactg gctaagcaac acattcacat tgaagacgaa cgagcgtatt
     8581 ttactcatga caacttatcc atacttgagc agttcattat gcaattaatt gctggttact
     8641 cagctgattc tgcagctaat ctcctgagac aagatcctgt gtttaaagct gttttagata
     8701 ggaaagaact cgcttctcaa tcttcacttt cccgattttt agatcgacta tctgaggaga
     8761 atatccatga acttcaagct ttgaaccaag aacttatcga taaagcacgc ctcatccgta
     8821 atgatacgga attaatcatt gatttagatt cgacccattc agatacattt ggtcaccaag
     8881 aacaaacgaa ttataataca cactaccaaa cctatggtta ccatccattg gtagcttttg
     8941 acggattgac tggtgacttc ttaaaagctg aactacgttc aggaaatcaa tatacatcaa
     9001 aaggcgtaaa ggagtttctc acacctttat tagagcacta taatcactct ctaccaaaca
     9061 ctgacatctt ggttcgtgga gacagcgggt tcgctacacc tggtgtgtat gattcgtgtg
     9121 aatcaaaaaa gagtcattat gttattcgac tgaagaataa tcgtagacta ggtcaaatag
     9181 ctgagaaatc agtactttat ggcgataatc aaaagtggga agaacgagaa gttcagtact
     9241 tctcgaccac ttatcaagca caatcctggt cacaaagtcg tcgcgtgtgt atacgctcaa
     9301 cacgtgaagc gggcgaacta ctctttcgac atgaatttat cgtgacgaat ctatcagaaa
     9361 atgtttctcc tgataccatc ttttctatct atgccaaacg tggcacaatg gagaacttca
     9421 ttaaagaagc gaaagctggc ttttactttg acaaaacaga cagtcctcgc tttttggaga
     9481 atcatgtccg aatgatgctc agcttgatag cttacaactt agtcaacttc ttaagaacga
     9541 ttggctttga agaagtccaa aagggaatga ccattcattc tattcgattg aagtttctga
     9601 aagttgctgg gaaattagtc caaacgggta gacgagtcta tctcaaatta tctagttatc
     9661 atgtgtatca gaatgaattc tacagggtct ttgctcgcct gaggcgagcc agtcaatgga
     9721 tctaatccaa caatctaacg atttttttac tgggaagtta tccagggaga agtatgctca
     9781 aaatgcctta cacccaaaat ttattcctca atatttttga acagtgaatg tttgaagtaa
     9841 ctttttagtc aaaatcagtt gatagggata ttagtgatac atatttcaat aaaatgtgcc
     9901 aaagaattaa agctatgaat tattcaggat tctatttatc tatctaacat atcctttatt
     9961 cttctacttt tctttcttta gcatataaga gatacgcata gtaatcaagc aaggattgtt
    10021 gatataaaaa gaacaaatac cgctattttg atttcttttg actctattac tcatataaat
    10081 aaaatttatt atacctgctc tttttgacta tatcctcatc tgactcctat acttcgtttg
    10141 tattaaaact taggaggaat cattcatgct tacagggcta aacttattaa ataatccttt
    10201 tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatacggaa tcactggtat
    10261 gcttccaagt actgtccaaa cattggaaca acaatctgtc caagcttacg gtcaatactt
    10321 aagcaaacag accgatttgg aaaaacgtat cttcttaatg aatctattca atacgaaccg
    10381 gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga
    10441 tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cacaagatgc
    10501 tgtatttctt tcaatcgacg aaccagaaaa tatcaaagca agtttgaaaa acgcagcaga
    10561 tggacgcgac attcgtttga tcgttgtgac agatgcagaa ggaatcttag gtatgggtga
    10621 ctggggtgtg aacggtgttg acatcgctat cggtaaattg atggtctaca ctgccgcggc
    10681 tggtatcaat cctgcacaag tcttgcctgt atcaatcgac gctggtacta ataacgaaga
    10741 attattgaat aatccattat acttagggaa tcgtcatgct cgtgtagaag gcgaaactta
    10801 ctacgaattc attgatcaat tcgttcaatc agctactgaa ttattcccag aattgttatt
    10861 acattgggaa gattttggac gaggaaatgc tgcaacgatc ttagaaaaat acgaagataa
    10921 aatcactact ttcaatgatg atatccaagg cacaggaatc gttgtactag ctggtgtatt
    10981 aggtggattg aatatatcag gtgagcaatt gaaagatcaa acgatcttga catttggtgc
    11041 tggtactgct ggtgtaggta tcgcaaacat ccttttagat gaaatgatcc gtcaaggtgt
    11101 acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacagggtt tattgtttga
    11161 agatacaaaa ggattgactc ctggtcaaat cccattcgct cgtaaacgct cagaattcac
    11221 taatgctgat gaattgacaa acttagaagc agttgtaaaa gcaatccatc caacgatcat
    11281 gatcggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggcggc
    11341 tcatacccca cgtccaatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa
    11401 agcaaaagat ttgattgaat ggacggacgg aaaagcttta gttggaacag gtatccctgc
    11461 tgctgatgtg gaatataacg gtgtaactta ccaaatcggt caagcaaata atgcgttgat
    11521 gtacccaggt ttagggcttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat
    11581 catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc
    11641 tatcttgcct ccagtagcaa aaatcactga attctctcaa acgattgctg aaactgtcgc
    11701 aaaatcagtt gttgcacaaa aattgaatcg tgaagaaatc acggatatca aagaagctgt
    11761 tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatt gttatagtaa
    11821 aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag
    11881 cagccagcca actactgctc ttttgccgct tctctttggc ttttttcata cgatacagaa
    11941 taagaaagga tctgtaaaaa tggcacaaaa aacaacaacc gcaactgcag ttgaggaaaa
    12001 gaaaaaagaa gggttttggt caacaaaaat taccggcgta agtttgcccc tttatttgat
    12061 catggtcctt gtcctgatca ttgcaatggc tttaggaaaa ttaccgacga atatgattgg
    12121 cccaatcgct gttttggtca ttttcggtaa tttatttcat tttatcggaa caaaaatccc
    12181 tatagtcaaa agttatttag gtggtggctc cgtttttgcg atctttgcct ctgccgcgat
    12241 tgctacattt gggatcttac ctgagtatgt agtaaaatcc acagaaaact ttgtcagcaa
    12301 tatgggcttt ttagactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa
    12361 ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt
    12421 cgtctccttt tttgccgtag gactcgtagg tatgcttatc ggaaatggtt ttgcaaattc
    12481 cgttttatat atctcattgc ccgcaatggc tggtggtgtt ggagccggag ctgtaccttt
    12541 atcaacgatt tatgcaaatg ttttaggtac ggacgcttct tctatcattt cccgcctgat
    12601 tcctgcgaca gcaatgacaa atgtcctagc tatcattggt gcagcgttag ttgctcgtgc
    12661 gggacaatca atgccaaaat tcaacggtaa tggtaaatta atggaaaaag gcgaccttgg
    12721 cgatggaaaa cctagaaatg taaaaccttc cattccacaa ttaggtgtcg gtttgatggt
    12781 atcattgact ttcttcatct taggacaaat ctgtaactat tttgttccta aagtccatgc
    12841 ttacgcattc atgatcatta tcgttgttat ctgtaaaatc gcaaacctat taccagaata
    12901 ttacgaagat gcagctatca tgttcaacaa cttgattgtc aaaaacttga cagcagctgt
    12961 tcttgccggt atcggtattg cgttattgaa tctaaatgta ttggcttctg ctttgacatg
    13021 gcaatttgtt gtcttatgtt tgacaagtgt tatcgcgatt tcagtagttt caggatttgt
    13081 cggtcgtttg tttggtttat atccaatcga atcaactatt actgctggct tatgcaacaa
    13141 ctcgatgggt ggtacaggaa atgtagctgt cttatctgca gctaatcgga tggaacttat
    13201 tgcttttgct caaatgggca atcggcttgg tggtgccatc gttttgatca tctcaggttt
    13261 cttgatgcaa ttactttctt agtcaaaaaa aatacctgat catcaagtat cggcttgatg
    13321 accaggtatt ttttgataaa aatttacagg attttcaaaa atcttttttg cattttatcg
    13381 gaatgataac cgatgcgttc ataaaatcgg tgtgcttcta ctctgtattc tgcagaatta
    13441 agtcttattg ctgatattcc tcgtcttttc gcttcttcta cgccagtcat caaggccttt
    13501 ccgattcctc ttttttcagc atgtttagac acagcaagtg ccataatatt gaacatcgga
    13561 tcgctgaaaa tcgattcata ggactccgct tgtacatagc cagctgcttt tttagaagta
    13621 ggatcttcat agaccaagaa aaaatttttt tctggctcct tcaacagttt cttcatttgt
    13681 ctcaccgtaa cggttaatgg tacatcatat cctaattgtt ccgcattgat ttctcgtagt
    13741 gcttctgcat ctcctaaacg catttcccgc aacatccaaa tcaccagcca atttttattt
    13801 ttttaaatga atttttttat cacagtatac tccttttttc acttaaaaaa agacttttca
    13861 gtgattttga cttgcttgaa tccagatcct tctaatgttt gcaagatgac gaaagcattg
    13921 taaagattga tcaaaagaac cacgatgaca atcgcttgaa aaataaaggt cacttttttt
    13981 ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata
    14041 acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc
    14101 aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt
    14161 ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa
    14221 aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa
    14281 tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc
    14341 aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg
    14401 aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca accgacaatc
    14461 tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg
    14521 aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca
    14581 agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc
    14641 agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat
    14701 atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt
    14761 cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaaaaccg ggctctactt
    14821 ttcagagagt ccagcttttc ttacacctta tcgattcttt tgttctattc ttcttccgcc
    14881 atgaatgtat tgaatacttc ttcgatcata tcccattctg cgtctgtttc gatttgttct
    14941 aactcgcctt ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct
    15001 tcatcttctg aaacaccagc tggatataaa agaacatagt tacgtccaaa ttcttcttgt
    15061 ccgtcaactg ttagcaagat ttcgtataat gtttcatttc cttggtcatc caccaatgtg
    15121 atgtgttcgt gtccttcgtg ttcgtgggaa tggttatgtt ctgtcataag attacctctt
    15181 ttctttttat tattaaaata attgcttacc tgagccgtct aaataatttt gtaaaatcat
    15241 cacagctgct aatttatcga tgactttttt tcgtttcgct cttgaagtat ttgcctgctc
    15301 tactagcata cgttctgcct gaacagtggt tagtcgttca tcttgataat caaccggcaa
    15361 ggagaatagt tctttgatct tctctccata tgacatggaa gcttctgccc ttggtccgat
    15421 ggaattgttc atattcttag gtaatccaac tacaaaacgg tctacttcgt actgcgccac
    15481 taattccttc aaccgatcaa aaccaaactc ttcttctgcc tcattgattc gaatgatttc
    15541 tactccttga gctgtccagc caaagggatc gctcacagca acgcctactg tcttagaacc
    15601 gacatccaag cccattactc tcataaattc actccatgat ttgagaggta gtctttgaca
    15661 attacttcca tgatttcatc gcgttcgtga cggcgaatca aatttcgtgc atcacgataa
    15721 cgaggaatgt aggccgggtc tccagaaagc aagtagccga caatctgatt gattggattg
    15781 tagccttttt cttccaaagc ccgataaaca atcgctaatg tttcgctcac ttcttttttg
    15841 cgactatcgt caaaaccaaa acgtacggtt tcatctgtaa aacccatgat tctacacctc
    15901 tttcccgcaa ggtaattcca gcttaccttc tacaccctta ttttactcga agaaagggaa
    15961 aactacaaac aaatattcca aacttctgaa taaattaaga aacacgattt gtctgatttt
    16021 tccttttttc tgtggctttt tcaaaagccc tctttccata aagtatacgc tatatttctt
    16081 tgaaagtatg tttttacaca attttttcat aaagttcata aaaaattcga attgctgcta
    16141 ctaatttttc gttttcgttg cttgccctgt ctcataattg atggtgtagc catcttgaac
    16201 attgtagata aagacattga attcaatttt tttgtcttca atgctttctg cttccaactg
    16261 cacacctctt gctaccagtt catttccctc gaaatgagga gtgacacgat agcgtacatg
    16321 atgatttgtt tccttgatat atgccgcaat atcattttcg tgggcaagca tgtacggagc
    16381 attcaacgaa cgtgtccctg tcattaagtt tttaatgtta ttattttgac cagtcaactg
    16441 gaaaccaatc aaatggctgc ggttgtataa ccattgtccg tcacttaatt tcttttgttt
    16501 ccaacctgtc ggatcaatat acaaaggttc tctcttttca gtaggaaaca agtcttttcc
    16561 caacatcgca ttggcttgtc ctactctgtt caatttgtct atatttgaat agctttccca
    16621 tgtccctttg gaaagactaa gatcctcttt tgtaaactcc ggctgattcc cattgatttc
    16681 tatttcttga tgttttccat catattctgg cacatccgct acttgagaag cttgcggtgt
    16741 ggaatcaaaa atggttgtaa tttgttggaa gatatctgaa tctttttgtg tggtggaaat
    16801 accgagtgcc cctgcaatca cagcagcgac gatccccacg atcgccttgg ttgttttttt
    16861 gctcatttct ttactccttt actaacagtt ccctaaatct taacatatgt tcccctataa
    16921 ataaatactg gaaagaaata gaaacagtaa aaaaataaaa ccagttttta gacggcttaa
    16981 gaatacaaaa aaagagacgg attcatcgtc tctactcaat cacaaaacac tatttacaag
    17041 gtatttatta tcattcaaaa aatttttctt aatactcaaa actaaacggc ttctgtacga
    17101 cctcttggta aacagtgttc aaaagcttgc atctgcggta ataagtccaa ccaagtcaaa
    17161 aacataagaa gcttttttgc ttgactattc ttattacttg atgcagggca gctttttcac
    17221 agcctctttt aagcgggtga cgggaatcga acccgcgaca tcagcttggg aagctgggat
    17281 tttaccacta aactacaccc gcgatacaaa aacagtatac gcctctttca ctccttcctc
    17341 aagttttttt attctttacg cttccgatat gttttgtagt aaaataaaac taatcaatca
    17401 attattggag aagatgctat gacgggaaaa gaattagatc tgttgctgga cacttgttta
    17461 ctcgctggga aaatcatgat ggaaagcaat gcggagatgt atcgggtaga agatacgatg
    17521 agtcgaattg ctttagcttc tggaaatttc cgtttggtca gctatgtcac tcaaaccggt
    17581 ctctttattg gtcttgatcg tacctcgacc atccgaatgg aacaaattac caaccgttcg
    17641 attaatttag aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt tgcttcagag
    17701 ctaacattag aagaacttta ctgcgaactg caagcaatcg accgagaccg ttctttcttt
    17761 cctgtctggt tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat ggtgttattt
    17821 ggcggaaaga tgaccgattt acctgtgact ttagtcattg gaggattggg atacgcagct
    17881 tatctatata gtttgaaatt ttttagaatc aaatttttat cggaattttt atcttcttta
    17941 ttgattggca tagccgctat ctacagcgta cgttttggct taggaagcaa tcaggatcta
    18001 atcattatcg gatgtgtgat gcctttagtt cctggagttc aaattacgaa cgctatccgt
    18061 gatttgctag ctggacatta tgtctcagga gtttctcgcg gaacagaagc gatgatgact
    18121 gctacgatga tcggttttgc gattgctttt attttccagc ttttttatta ggaaaggaac
    18181 acaagtatgg ggacgatatt tattcagttt tcttttagtt ttttagcgac tgcggcattt
    18241 gccgttatta caaatgtacc taggcgttca ttgatttgtt gtggtctttc tggtgctttt
    18301 ggctggatga tctactggat ctgtgttcaa ataggcggat ctgctgcttt tggttcattg
    18361 cttggttctt taggagtagc attcatcagc gatcttttct ctaaacgttt gaaaatgccc
    18421 gtaacgattt tcaatattcc cgggatggtt cctctagtcc ctggtggttt agcttatcaa
    18481 gcggttcgaa atctcgttac tgaatcctat cgagaagcgg ctcattatgc ggtccaagca
    18541 attatgattg ctggagcaat cgctttaggt cttgtattat cagaagtctt taaccataac
    18601 atccgtaatt tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa acggcttttt
    18661 aaataaacga agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct tgagcgatga
    18721 caaaatatct cacaaacatt gcgacaagta accgcaggag caataagaag taattatgag
    18781 tattaaaaaa agttttgtaa aaatagcttc tcacattttt acataaaatt gacttaatac
    18841 tgagaaactg acttttgaac accctctatc tgtaaaaaag gggctgcgac atgatttttg
    18901 tcacggcccc tcttctttat tcaaattttt tccaaaacgt tcgttgataa taaacgatcc
    18961 atagagcgga aagtattaga actaaaccga tcacaggaat attcactaac caaaatggat
    19021 aaaacaaagc caacattccg tataagacga taagaataat actgatgatc aatgaaccca
    19081 tcatagttgc gactaaaccc aaacttcctg aacctctgtt aaataattgg ttaatattgg
    19141 tccagttcag cagcagcaac cgataatctc tagcgaaata acgtagtgac aataagtagc
    19201 agccgaataa agaaccaaat agcaagctag ggataaaaaa caaaggcagc tgaaaagaca
    19261 gtccgcttaa taatgcgatc ccaccagtta aaaaactctg caatatccat ccgaatctga
    19321 acttttcttt taaatattgg ttcatagaga ttggcaaaga acggataaac agaaaatttt
    19381 cttgatccaa agaaataaga ttgctgataa aagaagtctg attaactgtt aagaaagcca
    19441 atgcgatccc taccaggaaa acaacaccga ttaacttatt atctaaatca gttaatttta
    19501 tttcgcctcc tagggcaaaa gcaagaataa aaatgattgg agttaacaag gaagaagaaa
    19561 gaacttgcat aatcaaatta ggttctttta ctaattgagc attgtaacta aataaaagct
    19621 gatgcacatt ttggttcttt ttatgggttc tttttatttt gaactcactg tttgaatcag
    19681 tcagttgctc tgtaagcttt ggtactatca gctgttttac agcaaggaaa aatcccagag
    19741 ccgtgatagc tagtattccc caacccaata aaccttgttg agaaagtggc tgactggcag
    19801 catcatagaa tggcaataat atcgaaatcg gatgccgatc tgttaactca ttttcaatag
    19861 tccccgtttg atgattcatc cacatgattc cagcaaatgc gatcagcatc gagctgccaa
    19921 gcaagatact agaaactgtt tttctatgtt ttttaaacac tgctgttcga gccaaaccaa
    19981 aaacaatcaa actagccaga caaaaaagaa taatcaaaaa tagagaaaac aaaaggattg
    20041 agataatgat agtaagcaaa atccctagat cagctcgcca tccggttaaa ataaacatga
    20101 ctagaagcgg aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc tttgctgaaa
    20161 aaacctgttc ttgtccaaaa ggcagaggca aaaatacttg caggtcattt ccttcaaaaa
    20221 agatattata aatgactgat atactttgag ttaaacctaa cataccaaaa agtgccacgt
    20281 aataagtaaa gaaacctggc aatttaacaa agttcatagc aaacatagca aatccgtaaa
    20341 tcagtaaaaa aatcagccct gaaaaaagat tttgagacaa aagatagata gttaatcgct
    20401 ttcccttttt tcctttctct cttgctttat tcgtgagctg tggattaaca tataaaagat
    20461 taactcgaat taattctaat aattgtcgct tatacatacg gtccatctcc tgtcgcttgc
    20521 cttccagcca ttttcaaata aatcttttct agcgtttcat caggtgagcc attcaataat
    20581 tcactaactg ccccctgata aatcaattca cctttcttca aaatagccag tttatcgcat
    20641 aactgttgtg cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc ttttgcggca
    20701 tgagccttca tcatttcttt caaatcaaat gctgcttgag gatcaagtcc ttgcaaaggt
    20761 tcatccaata cccaaatatc tggatctggc agcaacgtgc cgatcaaaat cgttttttga
    20821 cgcattccat gtgaaaaact tgcgattgtt tcattttgat gactatacat atcaaaaagc
    20881 gctgtcaatt ctgctaaacg cttttctttt ttttgtgtgt ctaattcata ggcagctgaa
    20941 atcaagtccc aatattctcc agccgtcaat tgtaaaaaaa tatctgggga atcaggcaca
    21001 taaccgattt tttgtttgat actcaatcga ttttccgtca atttcattcc atctactaaa
    21061 atcgttcccg atgatggttc aataatacta actaaacttt taatcgtagt tgattttccg
    21121 gcaccattat gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag agacaagtct
    21181 tttaatgctt gttttttccc gtaactttta cttacattga tgaattcaat catttctgta
    21241 ctccttttgt ttgttattac tttttattgt acccttcgtt attggtggag taaatgtcaa
    21301 aaagagtgca taacaaaaaa ggatagaaac gactaggctg ctacttgggt tgttctcttc
    21361 tacaggaaaa cactcttcta acaggtaaga tatgagtctt tgttttcttt aaaatgattc
    21421 attttctatc ttttcttgca taaaattctt tagtttatca atacttctct ttcccatctc
    21481 tagttgttct ccatttgcaa atgttactat ttcgtttact ctgctaatag atgtaatgtt
    21541 ttcagtatta atgatataag cttttaaatc tttacataaa tactcatatt cgagtgattt
    21601 tttatatttt ttaattgtgc cgtttacgag aatctcttca tctttcaata ctaggcctat
    21661 tttattttta taacctggca tgctcattag ataaaggatt tcatcgtaat ggatatagtg
    21721 atcgctatta ccataacgga cagttgtcca cctttttttc gtagcatgtt gaacaggata
    21781 gttttctttt tctttcaata agtaactttg gatgcttttt ttattttttt cataagtgaa
    21841 ttcttttatc aaataaccac taggcgtaat ctttcgatta ataacagtta ttgctttatt
    21901 cggatgagac gttagataaa caattttaca cttttcattc ttttctctaa gcatttcgcc
    21961 tagttcgata ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa tatctgagga
    22021 atagatttca ttatttttaa tgaattcaac aaattgtata acgttcaatc cctgaacgat
    22081 ctcaaatttc caaggactta ataaaattct ggattcttcc aaaatttcta ctagcatttt
    22141 ttcaaaaacc aaactatcat ccaaaataaa tatacgcgtt accataaaaa gtacctccac
    22201 tcgatatttt actatcaaaa tcatttcata caacttttca acatacatgt taggaataaa
    22261 ttgtaattct cttcacaatc ttttccaaca cctttgaaac cgatatcaaa tactttcagg
    22321 atgctgcttc taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa agccagacaa
    22381 ccgacgtcct tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc ttttaaaaag
    22441 aacgatacac atactgattt ttcggagcag agactttctc agcgtttgta acaactacag
    22501 atgggatttc acgcttgaat ggttgataat agtctgattg gatggttcct tctattttat
    22561 accattcatc atttttaacc gttactcctt ccggcatatg cgtcaacaaa ccaaagactc
    22621 ctgaatctgc cacacaatgg ataatgccga atcggaacaa gaagatatct gtattgctat
    22681 cgttcggtgt ttggtaaaca aatccttcat agctgatttt tttgccaatg aattcgctag
    22741 ggtaattata aattagctcc ataacttcta aatagttttc atctgtgatc ttgatagttt
    22801 gcttatcccc atacttttct ttcaactcat tcatttggtc taagtagtct gatttgttga
    22861 aataaatact ggtatccggc tttaagtatt gtgtctgcat ctccggatcc cctactgatt
    22921 ctttacttac tgggaaatta aatcctttgg cttctacgat cgtagtgtcg agactaactg
    22981 ttggaaacaa cgtccccacg attactggca aagctagcag tacataagcg attccacgtt
    23041 gataaggttt tgacaaccca tggtcatgat cgtgctcgtg tgccttttct tctgcttttc
    23101 cgtttttgac ccacaaatac agctgaacta aagccaaaag agcagataaa atcattgaaa
    23161 gaatagctaa gtagcgataa tggacattga tatattgatc gagtttgcca gaaacttgca
    23221 agtacatcat caattcgaaa tacccagata aaatcaaaaa ccgcagcata ttttccctcc
    23281 taaataaata atgtgaatcc aataacgcta agggcaatca gtccgatcag cgataaaata
    23341 aagcgtcctg taaaatatcg tttcatcatc agtagatttt tgatatcgac cattgggcca
    23401 aagaccaaaa aggcagctac aggtgctgtg ccgaacagac ttaacaaaga agatcctata
    23461 aacgcatctg cttctgagca aagagacatc gtaaaggcca ataacagcat gatgataatg
    23521 gctaaaatct ttgttgagcc gagcgttagc ataattctag ttggtaaata agtctgcatc
    23581 gcagctgcga ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa ttcgtcgatt
    23641 gaatgtgtca acacatgccc agtttgatgg atcagctgat tttttggttc attcttctca
    23701 ctttgttctt cttcatggac atgatgatgg tgattttctg ctactttaga cgtgattttt
    23761 tctttcaata cagcgtcttt ttgaaaatag gcaagccaga caccaatcac aagggcaaca
    23821 aaaaagctgc ctatcacccg ccagcttgcc atctgccaag aattgccaaa tgcgataaaa
    23881 gtggaaaaaa tcacgattgg attgatgatc ggagcagtca gcataaaagc aaacgctgtg
    23941 tgaactggta cgcctttttt aacaaattga tgaacgatcg gtacgatgcc gcattcacat
    24001 gaaggaaaga aaaatcctaa gatcgaacca gtaaaaatag agaagatacg attttcagga
    24061 agccatctct tcacacgttc tggggtcaaa aatacttgta aggcgcctga gataagacag
    24121 cctaataata cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt actcatctgc
    24181 aaaaccgaat ttggaataaa agaaaacatg agtttcctcc tttttatttc tagttcattc
    24241 gctttgcaaa tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat ttttacacaa
    24301 ctcttatttt ttaccaaagc acttcaattg actttttctg ttattcccat taaattaagg
    24361 tctcatacat atcacttcta tcataaaagg gagaataaaa atgaaacaaa ccactaactt
    24421 gactgaagtt caagacatac tgcaacagtc atccgttgcc gtcatttatc tttcgatgcc
    24481 taattgcagt gtttgtcacg ctgttcgtcc acgactagag gaacttctca cacattatga
    24541 tattccggca cttcatttgg atgcatttga aacaccagaa gtagctagta aatttgaagt
    24601 gctgactgcg ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc ggcaagcacg
    24661 gttcatcgac tttaaaaaaa tccgttatct actagatgaa ctgacagcag aagatgattc
    24721 cctttcttat gaagaaatct tccgaacaaa ataatcgttg tccaaacaga aaaaagcgat
    24781 tgaacaagtg aaagttttgc cttcatttgt tcaatcgctt tttattttta ttctgctgta
    24841 ttttcggaat gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg caattcattc
    24901 gtcaaatcaa cattttggat caatacatct gacggagcat tcaatcgagc agcggtaaag
    24961 ttcaagattc ctttgattcc tgcttccgtt aactgattga ctacttcttg tgctctttca
    25021 gcgggtatcg ttaaaattgc tacttcgatt tgttggatct tgatttgttc tgacatatct
    25081 tccattggat aaacggggat accgtctacg atgcgcccaa cgatttcctc attcacatca
    25141 aaagcacaac tgatacgtat gctattactt tgatggaatt tgtattttag taaagcactt
    25201 cccaagttcc ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt taatgtcttt
    25261 gcaaaaaagt gcataagatc ttctacatca taaccatacc cacgttttcc taattcgcca
    25321 aaataagaaa aatctcgacg gatcgttgca ctatctactt gaacggcttc acttaattca
    25381 gttgaagaca cttttttctt tccagcatta tttaaaatcc ttaaatagcg ataatacaaa
    25441 ggcagtcttt tagctgtcgc ctttggaata atatgatctt tcacgaaatg acctccacgt
    25501 attgtctaat tacatctttg atttctccat catagcagta tttataggaa aaaatcaatt
    25561 ccattgctca gatattgtga aattttattc tttattcatt tatttatcat ttctgcaaat
    25621 ctcgcagtat aatggaattt atggtagact actaattgaa aaatagtttt ttatttgagg
    25681 agaattcaca aagtagaaaa gaggattcca tgatattatt acaagccaat cagattgctc
    25741 gccattttgg tgcagaagtc ttatttgaaa acatacattt agaaatcagt acaggcgctc
    25801 gtgtcgcttt agttggcaga aatggcgctg gaaaatcgac tttattaaag atcattgctg
    25861 gaattgaagc acctgatgaa ggaaagatcg ctaaaaataa aacagcgact ctcggatacc
    25921 tcgctcagga cacaggactt gattccaatg aaacagtttg gaacgagatg ctgaaagctt
    25981 ttgaacaagt tagacaaatg gaagagcgga tgcgcgagct agagattttc attagcgaag
    26041 gtacgccaga cacacctgct tatagttctt atttaaaaga atacgaccgg ctgcaacacg
    26101 atttttcaga tcgaaacggc tatggttatg aaaatgaaat tcgttccgta ttgcatgggt
    26161 ttaaattcga ttcttcattt tatgatcaga aaatcgatac tttatcaggc ggacagcgca
    26221 cacgtttagc tttagctcgc atgcttttgc aacaccctga cattttgatt ctagacgaac
    26281 caacgaacca tttagatatc gagacacttt cctggcttga gaattatctg caaagctata
    26341 ctggtgcatt attgattgtt tcccatgacc gttatttttt agataaagta gtcaatgaag
    26401 tctatgaatt aagcagaaga aaaatgcgcc attacaaagg taactattcg aaatatctag
    26461 atttgaaagc agaacagtta gcaagtgaat ggaaggctta tgaaaagcag caaacagaaa
    26521 tccataaatt agaagacttc gttgctcgga accttgttcg cgcctctacc acaaaacgag
    26581 cgcaaagccg cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct caaggggatg
    26641 aaaaacaagc aaactttcta ttcagtatag aaaagccttc tggaaatgta gtcttgcaaa
    26701 tcgaagatgc cgctatcgga tatgataatg tcaccttatc tgaaccaatc aatctagata
    26761 tccgtcgtca agaagcgatt gctctagtag gtccgaatgg tatcgggaaa tcgacgttgc
    26821 tgaaatcaat cattcaccag atccctttta tccgtggaaa agaaacgcta gggacaaatg
    26881 tcaccatcgg ctattacgat caaggtcaag ctgatctgca tacaaacaag acgatcctag
    26941 cagaattgtg ggatgagcat cctaccactc cagaaaaaga gatccgaagt gctttaggtg
    27001 ctttcttatt ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt ggcggtgaaa
    27061 aagcacgggt tgctttagcc aagttagcga tgaacaaaga aaattttttg attttggatg
    27121 aaccaacaaa ccatttagat attgacaaca aagaagtatt agaaaatgca ttgatcgatt
    27181 atgacggcac actgcttttt gtttcccatg accgatattt catcaatcgg atcgcgacaa
    27241 aagtcgtcga actctctgaa aatggcagca aagtctatct aggagactat gattattatt
    27301 tagagaaaaa gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat gagccagaat
    27361 ctgctcctgc tcccacgaac aaatctgatt attaccaaag caaagaacag caaaagctta
    27421 tccgtagcct tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg atcgatgaat
    27481 ggatcgatca gttggaacaa gaaatggtcg cgccagaaaa cctggaagat cacgtaaaac
    27541 tcaatgagct gaaccaagat ttagaagctg ctagacaaga tcaagaaaca aaattgacgg
    27601 aatgggaaga actaagcttg gaattagaag aatatgaaaa ttaacgaaaa aaccaaaaca
    27661 gaaaccattc atgctgtttt ggtttttctt tttttatttt atcaaacaaa aattccatat
    27721 ttaccttttt caccaataag aagaccaatt actagtcatt ctcactaaag cttctaaata
    27781 atagtaatca ccccaaatac aaggtgtatt taccccatta tttttgggct tattataaac
    27841 agactgtgtt aaaaagccat cttcttcttc cgttgtagca tatctttctt tcaattgtcg
    27901 cataattttc caaacagcag actggtataa gttcttttct gttttgtcta atagacctga
    27961 ttctattaat tctaataatc cacaaaccat gatcaccata ctagatgtat ctttttcttc
    28021 tctgctagaa tcatcaaaga tcaaatccca ataacaaatg gtatcttctg gcaaccgatt
    28081 cataaaataa ttggctaata ttttgcttaa atcaaaatat ttttgttctt tcaaataacg
    28141 ataacttaat gcaaaaccgt aaatccccca tgcttggcct ctagcccaac aggaattatc
    28201 actataacct tgatgcgtac ttccatatct tggttttcca tctttgacat ccatataaaa
    28261 tgtatgataa gttgaaccat cttctcttac taaataatct gctgcatttt gaatatgagc
    28321 ctgagcagct tttttatatt tttgatttcc agtttcttca ctcgcccaat acaataatgg
    28381 aagattcatg caacaatcca tgatcattct accttgttgt gaaggatcac taggatctcc
    28441 ccaagcttgt ataactcctg ccttctccca aaaacgctga agtaatgttt ctgcagcatc
    28501 aattgaagct tccttcattt ttcgatttcc cgttatttta taatctgcta cggttgataa
    28561 agaatacaaa aaaccaagat cgtgatgatt agtgataatt tgacagtcaa tcctgtggat
    28621 aaaatctata tctgcttttt cagctgtttg ttgaaataaa ggatcacccg tcttctcaaa
    28681 taacaaccaa ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt ctcttaatgg
    28741 ataacgtcct tttataggac actcgcttgg gaattgatac tgactgttac tgatacgttt
    28801 gattaacctc tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt ctttttccat
    28861 taattcttta taacattcat ttatccattt ttctggatgt attatctctt caaacatcac
    28921 tttatcaagc atttccccac cagcctttag cataatttta gtcttttttt attgttttta
    28981 ttttggatag ttagcccggt tgtcccatca aaatcaattt tgggacgatt gttccatact
    29041 tctaaactat aatcccgatc ttgatgagca accactcctg ttccgagcca atgggttcct
    29101 tttccaaatg taccctctaa tccaggaata aaagtgtttt cccatgtcat caagtttgta
    29161 ttgggcaaac ccataatagc tgtggatttt cgtttaatag tatcgtcact taaacagata
    29221 atacctgaaa agccagcttc actcacccta tagttttcct tgtcttcttc tatgattatt
    29281 ccttctttct caggagaaca ttcaatagca aaccctgtct catatagaac atattctgta
    29341 tctaattcta ctttgtgtac gcgaatctgc cagccttcaa aaggaataag ccaagttctg
    29401 atttttgcct gtaaagtagg ttcccatgta gaggcaagta cctctttctt acatacaatg
    29461 tctcgtacat tatttcgaac gatgaactgt ccataggttt tgatgctctc attcttaaaa
    29521 ctggtcgtga tatatgtgtc cgctcgctga atccctagca ttgaatccat tgccatttct
    29581 gctttcaatt gattcgctcg cggtatcgaa aaaccaaatt gactagaata agcaaatttt
    29641 aaatattttt cattggtcaa tgtatggaaa tttggcccta attgtcccgc attcaaaaaa
    29701 aatgtatgtc cattatcatg ttgtatgagc aaattggctt ctgaaagaag tttagttcct
    29761 tcacgaatag gcataggcat ttctttagcc gtccaatact catggtcttc aggtagcgcc
    29821 aacaataaaa aaatcttatt cagccataat ggagataacg tactattata gggttcagtc
    29881 aatgtcaatt gagggtaggt ataaccaata ttcaggatac ctttttcatc aaatatttct
    29941 ttttgcatcc accatcttat attccgattg actaacccct tgatttcacc taatttaaaa
    30001 ggcaaaagat cggcccacaa catagctgac caaaagcttg aaactgcaaa acgataaatc
    30061 atgcttcgac caaaaggcac atttgcacca tcttccgcaa aaaaatgaat atagtctcta
    30121 gaaaaatcaa tagctcggtt cttatatatc tggctaatgg gcttttctgg ttctagtacg
    30181 ctataaatca aaccataata atgaaatgca aaaggattat aataatcttg gcgacctctt
    30241 gaactgtctc gataccagcc attttgaaga taacaatttt ctatattatc aaatgcttct
    30301 ttcagtgctg attctttaac tggataacca aatttttgga taattgttcc gacaattatc
    30361 ttaaaaaatt gccaattacc atctgcacat tcatattcaa aaatttgcga gaaccaatga
    30421 ataatctggc ttttttctcc tgtatctaat tttttccata ataattgttc atgatgatat
    30481 aacgctaata acattgcggg catttctacc atacgctggt ctctttcatg tatttctccc
    30541 caataatcgg gatgggaagg atctgttcca gcttttattc ctgatagaat taatttcaac
    30601 catcgttcat ctaattggtc aattgcgggc cccgctcccc ataaaacccg agagaatcct
    30661 tccattcctg caattttatt tccatatcct accccttgat gtatataatc aatttgtgcg
    30721 ccacttgctc gataaaacgg ctggagtggt tgatttatct ttttaaataa gtcaaagtaa
    30781 tcctgtctgg tccttaatag tggtactgga tgtttcattg tattttctcc tctttttact
    30841 accaaatagc taattccgac tgttctaatc gaagtaagcc ttcaataaaa aaataatcag
    30901 aatagatcag tggcacatta taagtcagtt ctctatggta tgcttctgct ccgcctccaa
    30961 tgatcccatc ttcatccata ttccaatttg catagtgctt ttctgtcttt tggagtatat
    31021 tgatagcagc ttcatgatac atttttgatc gtacagagga aacatatcct gctaattcca
    31081 gaagaccgca tgcagcgcat actccagcac tggtatcaat attcgaatct tttacagcaa
    31141 aatcaatgag aggaatataa ccagtttttt cgattttctg aataaaattt actgctatct
    31201 tttctgctat agctagatag tacggatttt ttgtgtaacg ataacttaac gcgaatccat
    31261 ataaagccca tgcatttcct ctgctccaac aagaattctc attgtatcct tgcccgccaa
    31321 gtaaccgaat atatttccct gattctgcat caaattctgc gatatgcccg gttgatccat
    31381 ctttcctcac tagatactta gctgttgtat ctgcatgctg ccgagcaatt ttagagaacc
    31441 tcggatcacc agtttccctc gaagcccaat acagtaatgg taaattcatc atactatcaa
    31501 taatcatcca accaggttta tggtcattcc atgctactaa atagttccct gaaatattat
    31561 agcgtccagc aagtaccgta gctgcatgta accctagttg tcgactatgt ttgttttgag
    31621 actgttgata atgtgcaata gccgatggta agaataaaaa acctacatca tgatgaacgt
    31681 ctgaaaaatg gtacagggca gattctaata attgttcttg ttgtattgct gcctctttga
    31741 acttttctcg ttttgtcttg tgaaagagct gccataaaat accactccaa aaaccattgg
    31801 tccaccagtc gattcctctg tcctttatta cgtctgtata ttttccgttg taagaaatat
    31861 atggaatttt attccccatt ctgtctaatt ccgcatctat cttttcagaa gctacattca
    31921 aaatttttct tgaccattcc ttcgtctgtt cattcatgga tatcccgcca ttcttttaat
    31981 ttttatgaat gtggagttgc tcctagaacg aactagatgg ttaacattcg ttattttttt
    32041 tacttatttg cagctccaat gtcattctcc ttaaatttag actgcgccca cacataaatc
    32101 aagaccattg ggatcactga agttgctaaa attagcataa ttgcgccata attaattggc
    32161 tcacctgtac tagaaaccgt catatttttg ataattaagg gaaccgtata tttatcttta
    32221 tctgaaagca tgactaatgg aaatagatat tgattccaac ttcccataaa gataagtaaa
    32281 ccactagtaa taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa aatcacccac
    32341 tcattggcac catcgattct ggcagcctcc atcagtgctg taggaaattg actgctatat
    32401 tgtcgcatca taaacactga agttgtactg gcaatcgaag ggatgatcac acctgcataa
    32461 gtattcgata atcctaaacg actcataatg taaaataaag gaatcaatgt ggtaaaaaaa
    32521 ggcaccatcc tagatagcat aactgtattg aacaacagtt tattccccat aaaacgatac
    32581 tttgctaatg cataacccgc cattgtagaa ctggctgtag ctgaaactgt actgattaca
    32641 gcaataacaa tcgaattcca aataatcaag ggataattat atctcgccat gatggcttgc
    32701 cagtttttta atagcatatc cccaaacttg aaattgattc cgccacttaa aatttcttga
    32761 ttactttgcg ttgaagctac aatcatatat aaatatggca tcaaaaatat taaggcacca
    32821 aacacaataa tgacgtacgt aaaaattctg ctttgtatct tttgtttagt catttttatc
    32881 acctccgaat ttaaattgaa tagccgataa tataacagat acaaacacca tcgtccaggc
    32941 aatagcagag gcttttccaa aatcaaccat ttcaaaactt gttttatata aattgactcc
    33001 tacgaccaaa gctgaatttt ctggtccatt ggaagtccca aagaatatat ttgggatatt
    33061 gaaattaccc aagccattga tagtggctaa aactgtggcc attaaaatga ttggctttag
    33121 catggggatc gttaataaaa agaattgccg aaatttcgat gctccatcta tttcagcact
    33181 ttcgtatatc tctttagaaa tcccttgaag accagctaaa aataaaatag tgttatatcc
    33241 tacactaagc caaatcatcg tgatgatcat tgatattcgt atccaaaaag gatttgttaa
    33301 ccactgtaca ccgttaattc ctagcgcatt caaaaatcca ttgaataacc cagtcggatt
    33361 gaaaaaagtc atgaatacgg aagctacagc caccgttgat gtaatggttg gaatataata
    33421 gacagttcta aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa cagcaaagta
    33481 aatagctaat agcatcgtta ttggaattga tcctgccatg ataatcaaaa cattcgtgta
    33541 agatttaata aacaatggat ctcggaagat agctagataa ttgtcaaatc caacaaattc
    33601 aaatgagtag ccaattttca taaaactaaa agcaaacgag ataatgatgg gaatcaaaat
    33661 cgtcacaata aagaaaataa caaaaggtgt gaccaatcca taagcacttt ttttatgaga
    33721 cgtatctgtt gcttttttct tgctgttctt tattgtcttg cttggtaacg aagattgcat
    33781 catcgttaag tcctcctaat attttttaat caccagttaa ttttacgccg tacttatgag
    33841 caaaattttc tgcttctttt agatatgaat tttcaaaatc acctgtttgc caatagttat
    33901 atgcagcggt agtcaaataa gacgtagcat ctgaataagt ggctggtaaa acagtatttg
    33961 atgtattttc attgatttta ttcaatagtt catagtattt ttgtcctcct tagtagtcaa
    34021 actttttctc agctgcttct gtttgatagg ccaatgagtt agatgtagcc acgcccagct
    34081 ccagtgcgtt ggctaagcat tcttcatctg tcagcataaa tgtaagaaat tgttgtgcca
    34141 cttgtggatt tttggcatct ttagggacat aaaaacttga tcctcctgat actgcacctt
    34201 ttcctggatt ttcttttgaa aaaggaatca gtggagccat gcgccatttg tcagatgcat
    34261 caggaaaatt caaagacata ttcgtagcat accagccgcc agcaacaaac attgcagatt
    34321 cttgaaatgc tttctcagca tcttgtgcac cgtagaaatg tacaatatct gagtcgatca
    34381 tttctttgat taactttgca gcatgtttcg cttctttagt attcagacgg atatttcctt
    34441 cttcatcaag caaagattga ttctgctggg ctagcatgtt gataaacaat tcttggtccc
    34501 cagattctgg caaggcaatt ccaaaactac cagttttttc ttttaatgct tttgccacct
    34561 ggatatattc ttcccaagaa ctgatttctt caaaatctac acctatttca gcaaattttt
    34621 ttggttgata ataacaaagt gctggggtaa aatcactagc ccaaggaata atcgtttcat
    34681 tactcgtttg ttgacgcaaa atattcaatt tttgatcata aaactgatcg ccatatttgt
    34741 cataaaatcc atattcatca gcactataaa aagcatcttg aaatttttcc agcacacctg
    34801 ctgctgaagt gtctgccatg gagataatat ccggcatatc ttcgtgtgtt accaattttg
    34861 gggtgatcat ttgtactgtt gctgttcccg aagctacatt cacaattttt aagtcaatat
    34921 ttgctccagt tttgcggtta tagatttttg cagcattttc aacatagttt gtcagtttgc
    34981 cgtttgcgac atatacttct aatgtagtgg ctttttttat cattggatct gcgatgtttg
    35041 ctgttacttc ttcgttagtt tttccacatg cacttaagct gaataatagg gtaatgttta
    35101 gcaaagctat caccaatttg tatctttttt tcatgtctat cccttctttc ttttcaagtt
    35161 cttatataaa acgctttcac ttatgtatat taacggaatt agta
//
LOCUS       DAJSVD010000019        35092 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSVD010000019 DAJSVD010000000
VERSION     DAJSVD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.019"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..486)
                     /locus_tag="ON498_000794"
     CDS             complement(<1..486)
                     /locus_tag="ON498_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939090.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MC"
     gene            complement(507..736)
                     /locus_tag="ON498_000795"
                     /pseudo
     CDS             complement(507..736)
                     /locus_tag="ON498_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(759..1565)
                     /locus_tag="ON498_000796"
     CDS             complement(759..1565)
                     /locus_tag="ON498_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939091.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            complement(1576..2220)
                     /locus_tag="ON498_000797"
     CDS             complement(1576..2220)
                     /locus_tag="ON498_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939092.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            complement(2236..3441)
                     /locus_tag="ON498_000798"
     CDS             complement(2236..3441)
                     /locus_tag="ON498_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3939093.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMS
                     IINTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            complement(3441..3818)
                     /locus_tag="ON498_000799"
                     /pseudo
     CDS             complement(3441..3818)
                     /locus_tag="ON498_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            complement(3949..5826)
                     /gene="ltrA"
                     /locus_tag="ON498_000800"
     CDS             complement(3949..5826)
                     /gene="ltrA"
                     /locus_tag="ON498_000800"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3939094.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(6514..8208)
                     /locus_tag="ON498_000801"
     CDS             complement(6514..8208)
                     /locus_tag="ON498_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3939095.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(8230..8823)
                     /locus_tag="ON498_000802"
     CDS             complement(8230..8823)
                     /locus_tag="ON498_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939096.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(8824..9156)
                     /locus_tag="ON498_000803"
     CDS             complement(8824..9156)
                     /locus_tag="ON498_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939097.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(9234..9776)
                     /locus_tag="ON498_000804"
     CDS             complement(9234..9776)
                     /locus_tag="ON498_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939098.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            complement(9837..12215)
                     /locus_tag="ON498_000805"
     CDS             complement(9837..12215)
                     /locus_tag="ON498_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939099.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            complement(12212..14824)
                     /locus_tag="ON498_000806"
     CDS             complement(12212..14824)
                     /locus_tag="ON498_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3939100.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            complement(14824..16878)
                     /locus_tag="ON498_000807"
     CDS             complement(14824..16878)
                     /locus_tag="ON498_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939101.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            complement(16947..17249)
                     /locus_tag="ON498_000808"
     CDS             complement(16947..17249)
                     /locus_tag="ON498_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939102.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            complement(17267..17509)
                     /locus_tag="ON498_000809"
     CDS             complement(17267..17509)
                     /locus_tag="ON498_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3939103.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(17506..17961)
                     /locus_tag="ON498_000810"
     CDS             complement(17506..17961)
                     /locus_tag="ON498_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939104.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            18149..18340
                     /locus_tag="ON498_000811"
     CDS             18149..18340
                     /locus_tag="ON498_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939105.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            18485..20542
                     /locus_tag="ON498_000812"
     CDS             18485..20542
                     /locus_tag="ON498_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3939106.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(20578..22668)
                     /locus_tag="ON498_000813"
     CDS             complement(20578..22668)
                     /locus_tag="ON498_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3939107.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVISEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(22665..22925)
                     /locus_tag="ON498_000814"
     CDS             complement(22665..22925)
                     /locus_tag="ON498_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939108.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(22938..23699)
                     /locus_tag="ON498_000815"
     CDS             complement(22938..23699)
                     /locus_tag="ON498_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939109.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(23715..24467)
                     /locus_tag="ON498_000816"
     CDS             complement(23715..24467)
                     /locus_tag="ON498_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3939110.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(24480..26456)
                     /locus_tag="ON498_000817"
     CDS             complement(24480..26456)
                     /locus_tag="ON498_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3939111.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            complement(26509..27180)
                     /locus_tag="ON498_000818"
     CDS             complement(26509..27180)
                     /locus_tag="ON498_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3939112.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(27262..28428)
                     /locus_tag="ON498_000819"
     CDS             complement(27262..28428)
                     /locus_tag="ON498_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3939113.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(28434..29123)
                     /locus_tag="ON498_000820"
     CDS             complement(28434..29123)
                     /locus_tag="ON498_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HCR3939114.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(29250..29489)
                     /locus_tag="ON498_000821"
     CDS             complement(29250..29489)
                     /locus_tag="ON498_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939115.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(29556..29795)
                     /locus_tag="ON498_000822"
     CDS             complement(29556..29795)
                     /locus_tag="ON498_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939116.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(29909..30250)
                     /locus_tag="ON498_000823"
     CDS             complement(29909..30250)
                     /locus_tag="ON498_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3939117.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(30237..30566)
                     /locus_tag="ON498_000824"
     CDS             complement(30237..30566)
                     /locus_tag="ON498_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939118.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(31157..31489)
                     /locus_tag="ON498_000825"
     CDS             complement(31157..31489)
                     /locus_tag="ON498_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3939119.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(32326..33366)
                     /locus_tag="ON498_000826"
     CDS             complement(32326..33366)
                     /locus_tag="ON498_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3939120.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKRLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(33715..34041)
                     /locus_tag="ON498_000827"
     CDS             complement(33715..34041)
                     /locus_tag="ON498_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939121.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(34028..34831)
                     /locus_tag="ON498_000828"
     CDS             complement(34028..34831)
                     /locus_tag="ON498_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3939122.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         9934 a         7102 c         5414 g        12642 t
ORIGIN      
        1 acacattaat gtcattactg ccgtgaaaat atttgacgcc gcgatctgtt ttggctcact
       61 taacaagtaa taggccaccc catagaccac caccaaataa ccgattaaag taaccataag
      121 tgaagccata taaataaaaa gttttcctat tggccataat tgcaatgccc aaaattcctt
      181 tcgtttgcta tttcttttca gaggaagcca tgtgtattca atccagtcat tggattttat
      241 ttcgatttct tccttaaatt ctttcttctt ttcttgccaa tgagggggca taatttcgtt
      301 gatctgtata cataaataaa gtaacatgac acacgataac gtactcatta accccttaag
      361 taccagatct tcgtcagaga acaaaagaaa aagcgttccg acgatcacgg acacactgac
      421 gagaaaccca acgataaata ggacgatata gctaatccat aaaaactttt gatacgtttt
      481 tttcacatag aacccacctt aattttttat ttattatcag ctgtaaaata ttttttagca
      541 gctatatata gagcttcaaa tgatctgaat tttaaggtat ctaaactctt tctaccattc
      601 ttcaactcag atattgttgt gttagataat tcacttcttg acatatttct ttagctttta
      661 caactttgag ataggcttta atcaatactt tctgctcagc gaaagtatct ttgccgacca
      721 agttcgctcg cttcatttct tttactccac ctattcttct aaatattttc ttttgtcata
      781 cgatcaattt tttcttgaag tttttgaatg ctctcttctt gatctttcat ttgcgttttc
      841 gatgtttcaa tttcttgctg gtaactatta atcttatttt cagtattttc ctgatcttct
      901 gcgacttgat aaggcaattg atcttttagt ttcttcacta attcttcatt caaatcaatg
      961 gttgcttgga aggattcaat ttgtttttgt gctgcttttt gttctttctt gtatcctttc
     1021 actttgtaat tgatcgcatg ttgttcataa tcttcgtctt tcacgtgatc tttgacctta
     1081 tcttcgtgaa tataaaaaat cagatcttgc ggttcttgca tatccacttt tggatcaatt
     1141 ttttcaggaa taatggttaa acgcatggcg atatattctt ctggtacatg cgttagcttc
     1201 actgcaaaat aatcatcaga caccttaatc actttggctc gtattgcttg ataatcccct
     1261 ttagctgtct ttacttctac gcgataatta agattagcca gttcttttaa caagctttga
     1321 tcttcatcat cgatcgcaaa ttcaaaaact aattgctggt tcgctccttt ttcttttgaa
     1381 accattgtca gctctttcgt gtttaaaaag gcgttcgtgc ttacctcatc tggtatatct
     1441 accttctgat tcgtataaat cggactagca agaatcaaaa agccgagaat cataaagatt
     1501 cccagccatc taagataact ttttcgtttt ttgaaacgtt gtctttctcc ttttttctta
     1561 ctcattttct tcctcctaac gagtgaaata actaaagtct atgactttcc attgaccatc
     1621 tacttgtttt tcagtgacag ttaatccacc ttttattttt tgtggtttga catctttgaa
     1681 ttgcggattg ccgattgtat aatcaacaaa tacgacaaaa ttttgttcct cttttacact
     1741 gcttgtttga gcgtaagttt gcacgtcacc taatgtaaag gagtaacttc cgtaatcatc
     1801 atgttggtga tcatcgtctg taggacttaa tttgtcgatc acttcttggg ttgcgacatc
     1861 ttttagtttt tccgttaatt catttctgct taatgattta tcaatggcca attgaccgaa
     1921 ctcttctgcc gtctttcttt gtgcttcagt tacttcctct tttgcttttg tgccatcttg
     1981 ttgcttcagc tgatcgattt cttggttttt ctcattgatg gtttgattta acttgtcgat
     2041 cttcgcctgt gcctcagagg aatttttttt gagcttctca gacgcgtttt tagccacttc
     2101 ttcaatttta taggtatttg ctgcttgttc ctctttcagc tgccaaaaca acgcaatatt
     2161 gccgattaaa ctaacgctaa ttgcagctgt taagacgatt tttaacgttt tattttccat
     2221 attttttctc ctgatctatt ttttactgct attcaaaaat ggtaacggat cgatccatgt
     2281 accatcatca atgaatgcat gtccttgtgc ttgacgccaa tccattcgag taaatcctaa
     2341 atgcaagtgt gctgtatctc gtatcccaat tacttgacca actttcactt gatcgcctac
     2401 tttcactcga gaatttcctg tactatttgc aaattcttga tacaccatat tcaagccatc
     2461 gtaattaatc acgatcacac acgcacccaa acctgaaata ccaggattgc caacgtacac
     2521 gacttttccg ccatgaaccg catgaatctc acttcctgga tgatcgactg aaccaaagtc
     2581 tagtccatca tggaacccgt taggtcggaa ttctcctcct ggatttttcc caaaaagttg
     2641 tcctccactg aacgaatttt tatctaagct acttcctggg aatggattgc cccagcttcc
     2701 tgtaatcgta ccaccagttc cgccgccttc tctccagtgt ttaatcgcaa actcatcgta
     2761 ctccatcaat ttatttgtat taataataga catacaattt ttataatagg cactgtcttc
     2821 agtaattcct gcagcggcat aaccgcctcg tcctaagatc aataagcttg cgccagggct
     2881 tttatttcct acacaagcat taaaattgga ttgtgtggct accataaagc cgtaatagga
     2941 aaatgcagct tttgagttgg gaaacttcat gtaccagcca ccttcaatgc caccaattcc
     3001 tcgtgcagtt cctttaggaa acagacaacc atcaaaccaa gtaatcccaa aatagttgag
     3061 atcatttctt gcggacgccg atgtaccaaa cgctgattcg taaccgtatt gcgaaaggat
     3121 cacacttggt aataatttat atttcactgc tgcacctacg gcatattgcg caatctcatc
     3181 agaaaaaggg cctcttcctt tgatttcttt ataaatcggt aagccttccg aatattctcc
     3241 tgtaaaagct tcgccaccac tgcttgaatc tgaatccgaa ttatcaatcg agcccgccgc
     3301 aagtattaga aatagggtca cgaacacagt gatcaaaata gtaatcaaac ctgcagaagt
     3361 accaaacaac cacaaaaata agcgccgctt tgcacgacgc ttaacatact gcttgattaa
     3421 actttcattt tggttttcca ttattttcct ccatcaaaca attttaattg acgtttgttc
     3481 ggactaaaat gaacacgaat ggtttctttt gagccaaaat tgatcacagc gtctccttga
     3541 ttttgttgag gaatggattc taattctgag ttcgtcattg atttacccac taatccttct
     3601 atatgacgta aaatagattc atccatacgc ataaagatct tcgtagaaga aaattcaaag
     3661 actttcttca gtttcgagaa gttatcagaa gacatatttt ctggcaccat ttcttgtggg
     3721 gattgtgtag ctaaaacagt cattgcttct actttacgca tttctcgttg aaagtttgtg
     3781 acataattta ccgtttcaat aaaatctgga ttaatcgata ctgataggta agagtttgat
     3841 attatctccg tcttttcccc cagtccacac cgtacgtgcg actttcaccg catacggcgt
     3901 tccctcgtct ataaaagaca ttctgttttc ccgtcctaca aatatttatt aagatatttc
     3961 ttcgttacca acttttaatc tcaactcatt gagtttttct ttttgttttg gatttaattt
     4021 tagatatttt agaagttcct caatcgtttc actttgtgta gcgtgtatta cccgatgaac
     4081 ctcctcagta acaatcacta ggttttcata gctatcactt ttgctttcat gataagggat
     4141 aatatgatga caatgcattt cagataagat aagctcctca cctgttactg cacatcttcc
     4201 cttttgagcg atgaattttg aaattcgatt gtccgcaaat tcaatcgttg ctctcttact
     4261 aagcactgga ttgctacaca accacgatat cttccattct tctacggttt gttgcttctt
     4321 atgaatggct tccctacctt cttcggtgta tttattaata tttctttttt tacacatcgg
     4381 atgtctgtgt tgaatatacc ctactggtaa tattggaaac cctcttatat atcgtatttg
     4441 tcctgaccgt aggtaaggct tgtatccttc gtactttccg tattgtcctt cttttgagat
     4501 tccttgttct cgtagtcgta tttcaaaact tggtctgagt tctcttgcaa tttttcgcat
     4561 tgctacacta caatgagtcg caattttgaa gtaattatga atcccaataa ccatactgtt
     4621 atagactcca atatagataa aggtatcttt gctattagga cttttttgaa ttcgtttgat
     4681 ttgttttttt agtttcactt taatcatgct ttgtgctttt gtacctatat gcgtattaac
     4741 aacctttttg ttacctttgg tcactaattt catcgtaaag cctaaaaatt cactttcttc
     4801 ttttgaaaga tttgtgattt ttgacttttc ttcagatatt ggtaacttta gacgctcctg
     4861 taaccacatt ttagttgcat agaaaacttt tttcgcttca ttataatttc tacagaaaat
     4921 tttaaaatca tctgcgtatc tcactaggta tatttctttt agagttgtac tctttctgag
     4981 cttgtgatag atattacctt tatcccaagc tccatttttc ctaaaatgat gttttagctc
     5041 tttggcatct ttttcctccc actgatttgc tatccaccag tcaaattcgt tgagattgat
     5101 gttcgcaagt aatgggctaa gtattccgcc ttgtggtgtt ccttttgttg ggtatatcaa
     5161 cttcccattt ggaagtacta cgggtgctct taacattttc ctaatgatta agagaagctg
     5221 tttgtctcgt atacccatag accagatttg tctcatgagt ttagtatgat tgacttcgtc
     5281 gaagaaacct ttgatatcta catctaccac atattccatg tgactttgat tcattcgggc
     5341 catacaatct gctattgcgt gttctgctga tctatttggg cggaaaccat agctgtgatt
     5401 attgaatttt gcttcacata ttggttctaa tacttgtaag atgcattgct gcgcgattct
     5461 atcccatatt gatggaatac ccaacggtct taatttacca ttatatttag gaatttctac
     5521 acgccttact ttccgagggt tataatgtgc gaatcgcttc ctaacaattt ctacgaattc
     5581 attacggttg agattttcaa tatctttaat cgttactcca tcttctcctc ttgtcacact
     5641 accgtgattt ctcttgatat tacgatatgc taggacaatg ttgttatcag aaatgataat
     5701 ctgcattaga ttcttgaaat tatctccctt caaacttttg gagtatatct tatcgaagat
     5761 ttccgtcatg ccataatatt ctgcatgccg caacttccta tcttttggac ttgtttcttt
     5821 tgtcatggtt tatcacgctc caattggctc gtgacctttt tagtcatacg agaatcccat
     5881 ggttgaacgg gtttcatgac ttttacagac gaatcgagcc tgttcctcca gttccattac
     5941 agaaccttca tcagtcgtga ctctactttc accatcacta aagtaagtta tttgtcattc
     6001 acatatttct tactaccact tcatcgagta tgcactctcc atgttgatgg cttcccctgt
     6061 tcccttatat ctatccacat gccttaactt agatgctagc ttttgccccc taacttctcc
     6121 tacgcctgta acgcagcatg gtctttcata acaacgactt ttactcaacc acaagttagc
     6181 acaatgagtg attttttgct cactgtctct cccatttcta ggagctatgc ctttcggaca
     6241 gtacattcgc tagttcgtca gtacgtttgt acatactcac catattaagt ttttttggac
     6301 acccgactta tcggatacac cctttacctc tagagaactt tcgtggtatt cactatttct
     6361 tttaccttac ggtatccttc agccgacttc accgagcttc acacaacgtt ggtctgcata
     6421 aactacttgt tgcatgtcgg agtatcagtg gaagcccttt gtggcgttac ccactcattt
     6481 gacttctcgt tataagagtt attacaatag tttttagact acttgcaacc cacagctttt
     6541 cactgcagaa caggtcgcac cattatggca ctcatcaatc atcgccaaga agcgtttgaa
     6601 gtcttgttct tccatttgtt tttctttaat taagcgtcgt tgtcttcgtc cttgaatcaa
     6661 tgcctgattc caaatcaacg tcatggccat gtacatctga cattgttgta cccctcgatc
     6721 taggcctgta atagagtcaa tgtcaaaaaa gaccaactgt tgttggtcaa ggtgtcgaat
     6781 cgtactatga ccatcaaaaa tcatgccata agtctcacat aaggattcca cgatagactc
     6841 catttcttct ttccgattct ttttctcttg ggaagcccct ggtagttcca gttgtcctaa
     6901 aaaggctaaa aattctttga acgtcggata agcttctgga tctttccctg tgacttgcgt
     6961 atcagtattt gaacgcgaat ttgaggaagt aaagccgcgt ggtactgttc catactcttc
     7021 ataaaaacga tttaagtagg tcttaaattc tgctttcatc gttcctttta gctgaggact
     7081 cactaactgt acctgataga ccactttgtc taagtgtgtt tgataggacg ctgcttcatt
     7141 gatcttcaaa tctttgactg atccatcgtc atataaactg gatgcttcat catacgtccg
     7201 tgtggcaaat atctctaaag gattgatcat tccgtcagat ccgtctaaac gcaccataat
     7261 accgttatat tcctttaaga aaggaataaa gtcttttccc ttttcaaagc cccaaataaa
     7321 gtctcctgca gctaaatggg cttccgcgat cattttaaga acggtcgatt taccagctcc
     7381 catcattcct agaagcaagg tattggtaga taaacgtaca ctatttttat acgtggcatt
     7441 ccagacaaat gggccgcctg tcatggtttc cccatattgt gtgcctcctg gatcttttag
     7501 cgtttggtgg tggaacggga atcctccgcc aatgtttaac gatggaagtg attgcccttt
     7561 gatatttcca aaccattcag cttgttcatc gaacatggta aacatcgatt gccaatctcg
     7621 tttggtttta tgcagataag tcaccgcacg ataatccatc ccttttagat tttcgatcag
     7681 ttcctgcgcc cgttcatcca cttcttggat cgtatttccg taaacatgta ctctcacttt
     7741 gacttgttta gaaatttccc cattagaagt caattttcgg gcatattccg ctagttccag
     7801 gtattcttcc gcacttttat ttcggtcagt cgcttttcgt tcattatctg atcgatcatt
     7861 taattcatct aatgcgcggt tgatttgatc aatcactttt tgcttttcat ctgtgctaac
     7921 atctagtgtg gccactgtat gaggattgtt catgagattg gccaaccaaa aaagagtagg
     7981 attttttgtg taatgataaa ctgtcacaca tcgtgaataa ccatttccat aggtcacata
     8041 ttcttcatca aaattcaacc caccttgtgg catcacttgt tttaagaaat ctaaatcata
     8101 accttttgat tttaaaatct ctatttcacg attaccaagt tgttttaatg gatcaatcac
     8161 cttttgatta ctttgcgttg aaaaagctgt ttttatcaca tctagcatga atcatttcct
     8221 ccatcttttt tattcgtcat tcatgttgtt cattcgataa agaatcttaa tcaattcttt
     8281 tttgtagagt ggttttaacc ctaaaatgcc actagatcgc attaactgac gatttctagt
     8341 ttgtaaatcc ttgattgtct ttccttcaat aacgaaaaag aagcttaagt caggacgttc
     8401 ttgttctgcc cgttttaatt cctggatctc ctctactaat ttcttttcat attttcttaa
     8461 tcgctgtaga tttcttccta gtgactgttc catacgattt ttatctaata actgccgctt
     8521 ttgcacgatt tgttccgtta gatccgtctc tgtggtcaaa ctaactaact taattgttgg
     8581 cacatacgta cgcatgacgg tggtcaactg attcattcgt tctagtttct cttgttcatt
     8641 taatccctcc acgtcagtgg aaacgacacg ataataacgc tgaaaatgat cttctctagt
     8701 aattaagact ccctcttctg tacaggattt atatggaaat aagtccaaga tagagcgctc
     8761 ttctggtttt ccaaactgca aaatggattt cttatcaact gaattttgtc ctttgactcg
     8821 catttattca tcctccttat acgtaccatg ataatttcgg ttaggatcta acgcatgata
     8881 gtggttctta tccgtaaaga aaatagattc gataatcgca aaaatttggc gttgaccagg
     8941 gttattatag ggtcgccaaa caatcactaa cgctatcgca agccataatc cataattcaa
     9001 gagtgcaaaa aagagtggta agtggacaag attattgact attttcgccc aaaaaaagca
     9061 gccaccaagc gttaatagat ctaaaaagaa aaaggcgaaa aacttccatt ccccactaaa
     9121 tcttggagga tttttcatat gaatgtattt atccataata taaccatacc ccttatattt
     9181 tgtattttta gaaataaaaa acactgtgat ttctcacagc gtttttctaa atattacaac
     9241 tctaattcat ttcctaaatc acgagaacga ttttttcctc gtcgtaattg ataattcaat
     9301 tgacgatttt cttcttttaa ttcaacaatt ttttgttcca gatttctttt cttttgaatt
     9361 aaatcataat tttgtgcgcg taattcttca ttttccaaat gaactcgttg catttcatcg
     9421 tttaatcgat ttacattctc caatgattct tgtatcgttt cttgcctaaa attaagtgag
     9481 ccaagttgac gatctttttg ttgtctttgc tctttcaatt ctgacgatat ttgctcaaca
     9541 gaatgaaata tttccgttaa ttttactgtg aatgtgggca tagcatcttt caaataagtg
     9601 aatccttctg aaaggtgtac aataatgctt tcggtatgtg ttttttgacc atctaaaata
     9661 gactgatgat ctttttgtaa actatccgta tctttaaatc ctttaatagt caccacaaat
     9721 atcgttaaaa atgctcccag tgcagttata aacgtagcta ttaaaatatc ctccacaatt
     9781 ttctcctttc cattttaaac ttgttttata aagaaaaagg cccttaaata ggccttttat
     9841 ggtttgatat ccttcaatag ttgttctttc aattcattgt tcctgttttt agtttctctt
     9901 gtcacattta tttttgtcgg tgcagtaaat gctacgtttg ctggtctgta tccattatga
     9961 actttattga ttggcttagg tgcagcttct tttgtcacaa tattttgaac ggacgcattc
    10021 tctgtttcac cactttcagc taaattgtaa gaaacagttg tcagatttcc attttcaggc
    10081 aaacgacttg atttcatttg aacagtacct gcaggtgatt gatgaacaaa gctttgatca
    10141 ttctctttga attgattcaa acgactatct ttcattggta aattttgtgt cttaggcaaa
    10201 ccaattttcg ttgatttttc agttccaact tcgatgccat tatcatttgg cagatcgatg
    10261 gaatcctcat ccgtttgtac ttcttgattt cgttttggta aatctgaaat gcgtttttcg
    10321 ttttcagaat taatgttaga tttataacga tattgttctg gatcggtttt tggtaactga
    10381 ccatgttgtc cttcttctga atctttttct ccgatcgaat tttcgttaga atgtaactca
    10441 tcttggtgat ctttggctga aggaatcgct gcaccttctt gattttcttt ttcacttgtt
    10501 gtatttgcct tcactgctgc attgacttgt ccttcaccat acgcatcttt tagtccttct
    10561 ttgactgctg aagctttttc tttttgtttt tctagtgctt cggatggatt cttcacagct
    10621 tcagcaaatt ctttagctct cgtgccgtct aatttctctt ctaaggtgtc tttggctttt
    10681 tctgtaagat cttcaactgt tccttttaac ccacgttcat tgacgtaacc aagaccttct
    10741 gcggctttag aaacaccatt tttcgctttc ccgaacattt cggaaactgt cttcttacta
    10801 ttaggtacag ttgtagcttt atcgtctgat tgttcttccc cgttatcatg ggtaatatct
    10861 tttcgatggc caaatccatt cttacgaata tcatttaaat ggcttaaacc actcttacca
    10921 gcacttgcaa ggtttttagc tccgctaccc acatttttcg caatatttcc taaggcaaac
    10981 atgctaagaa gagaattgct gccgtttttc agactcgtat caacaccgaa aatcttcgta
    11041 accgattgag agcctttgaa tagcgcaata gttagcacaa ttaaagcaac aacatacaag
    11101 aatccattga tctttcctac cgcatgcaaa tcaccgatac ctgaaagcat taatgtatag
    11161 attcgtaatt ctaaaatcgt aaacgccaat aacaagaagg attgagaaat atcttctatc
    11221 gcacttttca tccgttgtcc tgtctcaaag tccgttgacg cggcaattac taaatagaat
    11281 tttttaaata ctagctcaat aatgcaagta acgattgtaa acaaaataaa taaataggct
    11341 accgacaaag aagattcccc agccaaaata atccctggac tactaatgta tcgacgatag
    11401 cctgacgtat acacatattt tgctaaacct tcatcactaa tttgaacagg gatttctttc
    11461 ccattatcgt ctaactctag tcgatactgt aatgccttta accgatcttt ttgtactgta
    11521 ctaatatcac ttgattcaga cattttctta gcttgatcag gtgtaataat cccattgata
    11581 ttcgctgttt tgaaattttt cgctgttaaa gcatttcttt tatctgttgg taaggattct
    11641 actttattcg ggtcagtcgc tagtaatgta ctgacatcaa tcacattatt ttgaataatt
    11701 tgaaaagcag gtgaacccgt tttatctgct ttatttgctg cagtgacgtc tccataaaag
    11761 tttttactga tatttaaccc ttcatcgatt aaagaactac caccaaaaat caagatcatg
    11821 gccataaata aattaaccaa gatatttttg actttcggtg ggtgtttact cataaaaaat
    11881 ttcaagccta aataaattaa tgtgacgaca caaaaaagag ccaatacctt actaatcaaa
    11941 ctttgaaata gttcattaac accagcggcg ttcaataagt cttttaaatc aaagctttga
    12001 taaattgttt tttctaataa agtattgaga ttatataacc ctttgatcaa tccccataaa
    12061 aaagtattac aaatccaact gaatacatct tgatactgca gataattacc ataatcttgt
    12121 aagactttcg ttacggtttt gggatctgca tccccttctc cgaataaacc tgaccaccag
    12181 tcggctatgc cagagaaaat tcctccaatg atcattcttt caccgccttt tcagcttcca
    12241 ataacagccg tttcctaatc atttcagaaa tttcttcatt tttgatcaac ttttcaacat
    12301 attcttgaag acgatccgtt gcttgttctt cgtaaaattg aattaacaat tttttagccg
    12361 tctcattctc aaaagattga acgattttat aaaacatatt ttcttcgttt ttttcttcta
    12421 attgatcatg cagtccatga tagagatttc tagcatattc atctggtagg aaataatcat
    12481 aatcaatttg taaagcctta agatccaagt ccgcatgtgt acaatgtatg tctatatcat
    12541 tgatatccca cgatggattg aaatcttcaa tatattgata agcataagga agattcgttt
    12601 ccccatgatt aaaaatagga tacggccgta ttttatgacc tttcaaatct tgtcgtttta
    12661 aaggagcgag tacaatcgtt tcaccttcta aaaaagtaga aattctttca ggagttaaga
    12721 tacgatctga atcaacattg cgattgatat gagtattcat acctaatgaa ttgatggtac
    12781 tgttctcttg ttcaaccgtc ttattcccca cttttttaga aaattcttcg atggtattat
    12841 catctgtcga cataatataa atatgtgtct gacaattttc tttgataatc gctgcgtttt
    12901 gttctccata cttcgcataa agttgctgat aagattgaat aaacagatcc cataacatat
    12961 tacgacctgc cgtaaccgtc atgattcctt ccatgttgtc catcggtggc atatttccaa
    13021 attcatctaa aataaaatga atcctacggt gacatttatc tcctttagtc ttaacacatt
    13081 gcgtagataa ttctttatac aactgactaa gaaagatgct aacaatgata tggttagatg
    13141 gatcataatc aggcgttaac ataaaaatag ccattggttt atcggtatag tgcatccgaa
    13201 tagactctac taataggtta cttttcttct catctaatgt tagaacggct tctttttcta
    13261 gttctggcaa atgagtttct cctcgttttt ttcgataaac gatttttccc tcttcgtctt
    13321 tcaatacttt ttgagaaaac tcataccaag caacatattg ttttccttga tgattgtatt
    13381 ttaagcaaac atgatctccc tgctctaaat gacaatcaaa gttgatttca caaaaaccac
    13441 gatagccaac tttgactcgt tcctcgtgaa atttctttcc cttatgataa aattccacga
    13501 caatgcgttg atttaatagg ctttcatgta atttgagatc caaatacttc ggaaaaccaa
    13561 tggacttgta atcaatggtc gattgagaag tcatcttcgc aattttcgtt agggtaaatg
    13621 gattcaatcc tgtaattgcc gtagacaaga tattcccttt cgctttatca gatccaattt
    13681 ttgttgcccc atattgtttt tttgctatat ttccaggggg taactgattc atgaactcat
    13741 ctaaggcatt gagctgcttg aattcattct caggatctgg ataataatta aaaccgccaa
    13801 tttctgaaag tagttcaacg agattatttg gtgttaattt ggagtaatcc cctgtttgat
    13861 ccgcttgttc caataacgca aaaataagcg cattgacagc tttctgcgca ttttcgttaa
    13921 agtatttatt gtctgaagca tttgcatcat aataaatcgt attcgtaaaa gtattggtta
    13981 actgtgttgc ggtgtctatg tctcctcgct tccagtattt cacaatgagt gccaatggat
    14041 tatcactcat tgattgcagt ggatcggtga tattaaaggc atacacatca taccctctag
    14101 cttctagttc atctttacta gcaacatata attctgtttt tggatcatta acaaccattg
    14161 aactttgtct ttctgcacgc gacaaattgt caatggttgc taaaacttct atctggcctt
    14221 tccctgagcg ggaagtacca acaatacaag tatttactgt atccgtctga atataccagt
    14281 attttttata atgagtaatt ggtattccgc cataaccagg aaaacttccc tttgattctg
    14341 gtatttgcgg ataaatttgt tggatttcct ttaacgtttt taatcggtta tctccttttt
    14401 gcccatctgc tagcggacga aaccacatgt ccattttcaa tcccataagt acagctggaa
    14461 caacacaaat cgtagaaaca actaaacgtg tgcctaaact tgttgttggg gtcaaaggat
    14521 tccaaaaacc agttgaatca aaatggaata actgcggcat ttcttgtaac tgaaaaatat
    14581 tcactccttg taaacttcct ggaataatct caaatcgatg aaatgcttct ggtattcttg
    14641 taacaacata gtttaaagga agataagcta agccactata aaaaatcttt tgagaaatac
    14701 gtttgtcatg tttttctggt agctcttctc ctgaaggagt atagcgatag aaatgagaaa
    14761 gatacctatt cttttctaaa ttgaatcgtt tgtcttttcg tctcgttgtt tctctaatac
    14821 gcattatttc acctcatttg attctgaact ttcggaagtg tttgagtgta cagttgaatc
    14881 gcttgatcct tctttagcct tactaggatc actctctttg ctattttcca tacttgactc
    14941 tgctgttgac tgattggtac gattcaataa ttcttccaat tgttgttcat actcttttct
    15001 tgcttgttcc tgggcttctt ccttgattga cgccgttgtg gctacagaat ctgcctgtga
    15061 agattgattg atatgattaa aagcaaatac tgttcctcca cctagaagca agcttaaact
    15121 tgcaatacat cccaccatcc atccgttaaa ggaacgctgt ggtgaaggag ttaaatttgc
    15181 ttggttgttc actggcgttt gaacagcagc tactggttga ggttgtgcta gataattcaa
    15241 ctgtgcttgt cgcaatgtat ccatttgttc ttgacgttcc ttacgagcag ctgcttcttt
    15301 ttccttttct tgctctaacc gttgtttttc tatcgccact ttttgttctg caagatgctg
    15361 ctcacgtttt tttagttcta attgttcgcg ctctttttct tcttgtcgtt gtttttcttc
    15421 ttgttctttc aattcttgtt gttgaatttc atactctttt ttccattttg gaatttgtgc
    15481 ttgtaattct tgctggatct gcccgtgtct ttccataaga acttcagtcg ttgtttgata
    15541 aagtttattc agttgttcat ttaattcttt gctaatcttt agcttatctg tacgtaaacc
    15601 tcggtagaac tcttcttttc gttcttttat acgttctttt aacaagtttt ctttttcttt
    15661 cgataaagct tcttgattag ttttagtaaa gttattaatt ttctctatac acaaggaata
    15721 cgcattgtcg cgtttctttt cgtatcgctc tttaattttt tgtacttctt cttcaatcac
    15781 tcgttgttct tctttattta gttcctcttt tgttctctcc caattagatg actgttccgt
    15841 cgcatactca tttatttctt gatctgtttc ttgttgacgt tcttcaaaag tggctaataa
    15901 atcttcttgt gcaagctcat gccaatttgt tgattgaaca gactctaact gttctaaaag
    15961 tgtgccttta ccctgtgaga ttaattcttc tttactctct aaaaatgctt gataggtttg
    16021 gggtaattga tatttcgaat aaagctcatt taaaaataat ttattttttt ctttcatcca
    16081 ttcatgctct ggcggatatt gatccaaaat ataatcaaat acagttgcct tttgtatctc
    16141 tgtttcgttt ttctctactt cttcactgat aagtggttca atatattcct gagaatcccc
    16201 ttcagtccat gttggctctt cattagcttc aactagatca acttctactt cggccgtctc
    16261 ttcatcctcc attgtttctt caaagggatc tacctccaat tgacccatta catcgttctc
    16321 ttcctcaaat atcgatttct gagtcggaat atcagcttgt tcgtcaacat agactgtctc
    16381 tttttctttt gactccttct ttggaaagat tggtcgttcc catggtactt catcatttac
    16441 ttcgacttca ctcacttgac tcacttttct ttggtcgctc tctaactctt cttctaaaac
    16501 ttgtagcagt ttttcgtttc gataagactc ttccacactc atggtttcat ccgtttcctt
    16561 aaacacatca aggcggatca aatccatcaa tgtaggataa aaaccttctg aaatttgata
    16621 atccagtggg ccaaaaacgt attctgtgcc agccttttct atttttcctt ctttatcatg
    16681 taacgtgaga gtcaatttaa cagaagattg attctcttgt tctaacactt gtcctaacac
    16741 atgtagctgt tcttccgttt caatgacaga atatttaatt gttttcggta aaaaggcagt
    16801 acgctttccc actttcttcg ttgctttttt tattaactct ttatcttttt tggtattgcc
    16861 tgaaatcgta aagtacatga gtattctcct ttcataaaaa aggagtaaaa agtagccacg
    16921 tactttttac tccttctatt tattttttat agtccctgtg gggtttgtcc aaacattcca
    16981 aataacgaaa ccacaataga ggcggcagct aatataactc ctacccccat caatacataa
    17041 cctacgtgca tcttcccgtt ttcagctcct cttcggccaa agaagaagaa aatcccccca
    17101 agaacaatgg aggcaacggc agctacagcc gcaaaaatca tcagattgtt ttttaaacta
    17161 gttgctgcac cttgtaatgc tctaaaaata tcaaccgcag ctactacttt cggttgtgtt
    17221 aaaaaattca ttgtaattga ttcaaacata aaattgttcg ctccttttag tgtttaataa
    17281 tttcatcgaa aacaagcttt tcacgttcac gttgatagat atatccttct aagtccttgt
    17341 cttttccccg taataggtca aattgtgctt tttcaaggac ttcttgtttc cactgtgttt
    17401 cagatacacc gcgacgttct aagattttag acgcggcttt ttcaaatggc gttttagctt
    17461 ttgccactgc gattcctcct tcaggataaa taaaaagact agcaatcact cgctagtctt
    17521 ttgtaacttg tatccagatt tagtactgta ttcttttaat atttcacttt tatctcgatg
    17581 ttctttttcg tttgttggaa taaatttaaa cgtttctaaa ccgtctttgt tatagggaac
    17641 tattaaggct gttttaccta tgaataaatc tactccacct gaatcgatta ccttatatct
    17701 ttctaatcct ttatagtctt ttagcttctc aactgttatt tgtacttgtt caggatgcca
    17761 aacactttcg gaatattccc atgttgtatc atctataatt tttaaattat gaacaagtcc
    17821 gcctacttct tctttccaca ctgttcctgg ttttaacacg ccattatctt gactatcttg
    17881 cttatttttt tcactaccac atccacctaa aataacaatc atggcaatca acgaagtaaa
    17941 taccattatt aactttttca ttctattcct tctttcgttg ttttatttta gtatattaaa
    18001 taacgaaagt agcaacaaac ctcattttga tacacgggga acggatactc ataatttccc
    18061 acctttctat agaaactttt cctcttgata ttgtgattta ttacaaaaaa gagctataat
    18121 aaagaaaaaa tcaagaggga gcattgcaat gtacgctgat aaagagaaaa tcgaatggtt
    18181 actatttgaa actggcaaga cagcatatga aattagcaaa cattcttcag tcgcagaatc
    18241 aacaattgcg gatttaataa agaaaaaaag tgcgattgaa aaaatgcgat ttgataatgc
    18301 tgccaaacta accaaatacg ccgaatccat ttcttcatag catataaaca gccatcctta
    18361 cactcttcat agggcggctt ttttatttca aaatttccac ggtataccgt tgactacctc
    18421 ggtataccgt ggtatattat gaatgtaaac aaaatattta atcatttaat agggagttga
    18481 aaacatgaaa ttattaaacc caaaaactgt tatagctatt ggaacatttg ctgccacagt
    18541 tgctcttgct ggatctgctg acgctgcttc aactaacgtc atgtatcgcc tttataatcc
    18601 aaataaccat gaacattttt acacctctag cacaaaagaa cgtgatcacc tagtgaaaat
    18661 taagtggggt aattatgaag gccccgcttg ggaagcaccg actagcggtg gccaccttgt
    18721 ctatcgttta tacaataaag gccttcgtga tcaccattac acagctagtt gggatgaagt
    18781 gaaatggttg accaaaaatt acggttggac ttatgaaggg cctgcctggc gttctgccga
    18841 aaaaaataat aaaccgatct accgcctttt ccttccaggt gttacctctg gttcgcatca
    18901 ctacactgca agttggaaag aagtacaatg gttaaccaaa gactatggct ggaaatacga
    18961 aggtattggt tggtatggtg ctgacacctc taaacccaaa gtgaataaat cagctttaga
    19021 agctctttat acgagcgtta agggaactaa aaaaggtgac tataccgaca atacctggaa
    19081 cgcattccaa accgctttaa acaatgctaa aaaagtgtta aatgactcaa aagcgactca
    19141 aaaacaagtg gatagtgcca aaaacacatt agattctgct tacaagggat taaagaaaaa
    19201 accagctcct actgtgaaca aaacggaatt acaagccttg tacaaccaag taaaatcaac
    19261 tgctaaagga gattttacag aaggatcttg gaataatttt cagactgctt tatccaatgc
    19321 taaaaaagta ctagacgatt ctaaagcttc tcaaacccaa gtaaataatg ctaaaaatag
    19381 tcttgattct gcatttaaag ggttacaaca caaacctaaa ccttcagaat atgcggtaac
    19441 tgtccgtcat atgaatcgac aaactggaac gctgctaaaa gaagaacatg tctcagtaaa
    19501 agctggtaca agttacactg caaaagctag aaccgacttg actaactttg aagcaccaca
    19561 gtatcaagta aatgggaatg aaacacaaac acaaacgatt aacacaaata ccacatttac
    19621 tttttactat gatgaagtct ttctagttga agtgaaagct cgtccaaata aagtctcgga
    19681 tactgatcca aatatccaag aactctataa ttacagtaag agttatcccg tcgtttatgg
    19741 acaaagcatc acaattgaag cacctatgtt cactaatttt gttcttgatc ctagagaaag
    19801 ctctagcaac actgtgactt taaacaacgt tacaagtaat caaggaatca attttgccta
    19861 tactcatcaa tacaacgtca ctgtgaacca tataaacgtt gacacaaacg ctgttttatc
    19921 tactgaaaca caaacggttt acgagggaga tagtttctct acttcttgga aaaatatgac
    19981 cgatcaaaac tacttcttgt gtagaaatga cgattcatct gttaccgttg ataaaaatgg
    20041 acaacgtgta atcaataatg ttgaccaaaa tcgaacgatc actttcaaat acaaaaatat
    20101 ctcactaagt gatttaaaca actatgtgcg tcaaaaagaa cttgcttggt taaatagcta
    20161 ccgtcaacaa aatggcgttg ccccaatgca attcaatgat atcgttcaac aagctgcaga
    20221 tattcgtgca aaagaattac aagtaagctt cagtcattac cgacctggcg gcggtacttt
    20281 ccaagatctc ttagaaagct taggctgcta cggtgctaaa ggagaaaata tcaatagttt
    20341 ctacctttac gttgacgaga tcctttcaga tacaggagca gtaaatgcta tggttatgtg
    20401 gaaagatagc gctgctcata acgcaaactt gctttacaac aatcaaagta tcgttggttt
    20461 atcccatgct tatgaagttt ctgcagacgg atcactttct tcagacaacg tatttattag
    20521 tgccaatccc attttcaaat agaaaaacaa ggctgatttt cagccttgtt tttttgttta
    20581 attgaacttc agaactgcgt agccaaaagt tgctaacaca agtaatagta agctgaccgc
    20641 taaaatcgtt actaaatgtg tagaacctgt tttaggaaca ataacttgtg gcaagttagg
    20701 atcaacccca attgtcacaa catttgtttt attagaaaca ttatctactg ctactacggc
    20761 ttggttatca atattttttt ctgattgtaa tggagtttta gctgtaaata tcagctgaat
    20821 ttctcgtcca tctaaaagat tgttatttaa gaagtcagcg gaagcttccc acttcagcgt
    20881 tcttgaattg ctatcatagg ttagtgtgcc ttcagaagta atatcttttc ctttactctt
    20941 atctattact tgcgcagaac cttctttata tgtcaaacga gcttcaattg gatcttgaat
    21001 actaaattgt ttgtagtgat ctgacgcagc gtctacgcct tttgtgtgcc atttttgtgt
    21061 gactagatat tgtacttcag atcctgcgtt taaaggagct gtcggcgtac ccttcttacc
    21121 gtcatttcgt ccgtttacat cttttccatt ttgttgtact tctttcactg gaattttagg
    21181 gaaataaacg gtaatgatat ttgtatcctt ttctacttta ttaatgatcg acgttccctg
    21241 atttttggct acactatttt gattggccaa ttcattgact tttacattga tcactaattc
    21301 gtaagtttcg ccgttatact tcattcgatt ttttaatgta tcagctttcg ctgcatattt
    21361 tacctggtgt tttgttttgt cataggtcac ttcaccagat acatcgaatt ttttatttgt
    21421 accactttca acgtgtgcat cgacgtaatt tacttctttt ggtaaattat cgattaactc
    21481 aaatcgatcg taaaccgcta gtaaatccac tcctaatcga ttcaccttct gtttgattcg
    21541 atattgaatc gtgtctccta cttgcaacat tttatcgttc acgttatttc catttttatc
    21601 aacccctgtt ttttcaggtt gatcaggaac aacactaaag attgtcgcag aagataaagt
    21661 aaaccaggtt tgaacattag aagaaccaat cacaaacgtc ggatttgctt cagatatttt
    21721 aaaggacaca gtaccttgac caaacttcgg atctcctaat ttatcatgca aattattgaa
    21781 atcattatcc gcttgtgtca gcttagagct tcctcctggg tggccaccta ccacgttcag
    21841 attgttgtag aaagatttaa attctgaaat aaccgtatct tttctgacat accaatctgt
    21901 cgtattcatt ttgtcatagt gggcatattc tcctttaaga tccgtgcttt ttcctcctgc
    21961 agggttcaaa gagttaaaac tgataaatgt atctcctgaa aattgaacgg ggctaccgtc
    22021 ctcatatgtg gctactagct gcacattcat ttgtgttgaa tggtaaacaa ttcctgagaa
    22081 aagattgtct gagatccaaa gtactgttct ttttttagat ttactcattg caccattcgt
    22141 atttttatag ccatcgtctc gaaaatgagt ttttgtatag ttgttatcta gtagattcca
    22201 cggcaacgta tccgagtata agtgaatatt agatagaact agcttgatat tgactttttt
    22261 cccgttatac gtaccgattt ttccatagtc aatattgact ttggtgttgg ttgttacgcc
    22321 ttctaaaaca aggtttgttt caccgtattc tttttggaaa gaaggatagc gtatattttt
    22381 gtttccctcc actaatccta cgtagtcgtc tgtaaaacca cctttgttgg catcaacatg
    22441 atatttgtaa acttttgacg ctccgtttac agtaatattt gtttttgcat taggaacatc
    22501 ttttggaata ataaaattat tctttacgtt ctggattgga tttgacggat cgacaataac
    22561 tttttcagca aagacattgg ctgtttttaa cccaaacaat gtacctaaca tcatcattat
    22621 gggaaaaaca tattttaacc gtcttgtcca cgagtgttta aaattcatcc cttcatcccc
    22681 tcttttttat aaatgactag tgaaattccg acaataacga tcaaattgat cgtagtaaat
    22741 aatggattcc taatgagctt ttccataata tctagcggca tggaagcagc tggtatgttt
    22801 aaaatcctca ttaggtatag ttggtagctt ggcaaaatag atagtttaga aaataataaa
    22861 ccgcagtaca aaaagactgc ggttaaacaa gaaaaattga ataataataa tttgatcagt
    22921 ttcattttat ttcttcctta atctctagaa aacaagtgag taacaaaatc ccaacaattc
    22981 tcaaagaagt ttttctttac gccagtagat acgatttttt ttacttcttc tttaggttgc
    23041 gaagcttctg atttaggata aatccacaca tcctttaaga attcaccatt tttatcagaa
    23101 acaggtgtta atagcaaaat atcctcgcta ttagaaatgt tttcaggact tgattcattg
    23161 acaatataat aagcttgttt ctgatttgcc tgtacttcta ccgagaattc aaagacacct
    23221 gtttgttgat caattgtctt tgtttctcca ttagaaatta gcttcagttg atcactggat
    23281 agttttttca ctcgatcttt taattgggat tcaatttggt cagtagtgag atccttaaca
    23341 tccatctttt gtaaaatgtc cgatacatca taaacagaaa acttcgcgcc atttactcct
    23401 tcttgttttt caagcttttg cccctggttg tctaattgtt cggaagaaat aattttttga
    23461 acgtgaatgg tctgtttgac tatttgatcg tcttcagcaa ttttaggttc ttctgaagaa
    23521 tcaactgttg acgaatcgac ggtttcatcg ctgctttgaa tcgtgctgct atctgacgtt
    23581 tggattgttt gttctgtgct atccgtgctt tcttgtctgt ctgcaaacgt caactgtgtg
    23641 taggcagtga aagaaaaaag agcggctgca caaacaaata gccattttct atttctcatg
    23701 ttggttaccc tcctttattt tttcttttta taaacaaata gcgaaccaat gatcatgact
    23761 gaaagaaaaa cagtacttcc taaaagccat ttccaagata atttttgttt cttattttct
    23821 tcatctttta attctgattt tgtaaaggtt actcgcttcc catacactag gaaacggtgt
    23881 gtgttgatcc ccttaggcgt acatgtcatt agcgttactc gatcttcatt gggatttggt
    23941 tctaagtaag tgacaaagtt tctttgtagt tcgtcttttt gcacttcctc aatccgatcg
    24001 atttgataag cgtgctgctc accatcaact ttgatataaa acttttctcc ctttttaact
    24061 gaaggcaaat catcgaaaag attatcttta taaagtccac tgtgccctgc gattaaagga
    24121 tttttaccgt ttcctcctgt aatatcgcca gttccttccg taatccctac accattctct
    24181 aaaattttgt cggaagttcc gtcataaatt ggtagtttta aagaaatact tggaattgta
    24241 agaataccaa caggatcgcc taactcaatc ttagcaacat ccaaagaaga atccgtttct
    24301 gctgccgttc ctgttcgatc gattgcggca atctgctgat tttgttggta tttcgccacg
    24361 ctcgcttcag caggcatcaa aagtaagaca ccaacagctg aaagtaacca gaaagcaatg
    24421 atccatttac ccatgattcg ctttttctta gggtgtctga taatcattag ctgatccttc
    24481 taggctttct tcttaaagta aacaactgca gaaattgcgc ctgccccaag caacaagcaa
    24541 gagatcaata cgtaagattg gaaaccgttg ctaccagtca ttggtacgga agctttttga
    24601 ttatttttga tcgtgattcg gttttcttct ttgtatgaat ctttgtctac tttgaaagag
    24661 atctcttttg ttaatttttg atagttttca ggagcttttg tttcttccag tgagtaatct
    24721 ccttcagtta aaccagtgat ttcaaaatga ccctctttat ctgattttag gattgctgca
    24781 tttgctacat cgtctaccca aactgcttct ttgacaccat ctttatctgt atcagtaagt
    24841 ccaccgtagt acttaccttc tgcattctta gctgcaaatt ctgcgccttc aagcggtgtt
    24901 gctttgatac ctgttccaaa taggccgcta tcgactttta agaattttgc gccataagtc
    24961 acaacgtcag ctttcgtttt ttcgtggtgt tcttcctgat taaatggatt attgttataa
    25021 acgaaatcca cgctatttgt taatggattt gcgatttcag cactatcatt cacaaacatg
    25081 ttataagtca tttttagatc ttgaccatgt aaattcgcaa ctgtatcact tacttgtcca
    25141 ttatttacga taaaatcgat cacaaaacca ctatcagaat tttcgatttt atagtcagta
    25201 ttttctttta atacgactga accatcatta tttgttactt taatatctgc tttttcagca
    25261 acataagaaa gtcctttacc tgctgtgtcg ccccatttaa atacactgta aggagaagtt
    25321 tcacttttta caccatttac tgtaatccca tctgttccac ctgcgatatc tgtagggata
    25381 tgaattgtac cttgataatt cactgcgtca ccaatttgga aagaatgttc ttctcctgtt
    25441 ccatttgtca ctgttttatc gatcactggt gctttatagt tcacgtagga agcttttaaa
    25501 tcacttgtca cgccgtcatt cgtaatttca aaggttaact tattgtgcgc atcgttgcga
    25561 gcgtctgcgc ctaacaaata ttggtctgct gttggttctt gatctgatcc aactacaact
    25621 tctgaaggaa cttccacaaa gtagtatttt cctaacgtca aatcagttgc agtcagcttt
    25681 ccttgatcat ccgatacaag ctctcctaat tttgttcctt ttccaggttc gcccttgtat
    25741 aactcaaatt ttgccccttt taaaggtgtt tgtttgcttt gagctgtgcc atcgtcacca
    25801 aatttcgtta attcaaaact caacttagaa acaatgttct ttggatacaa atgaatatct
    25861 tttaaaaatc ctgatcctgt gttgtctgtc attggcgcaa tcacaaccat tggttttgct
    25921 ttttgtgtga ctaatccagc agcggattta ctttcataaa ccacatatac attccctttg
    25981 gaattgacat aggctggttg atcagcaaaa gtgatctcac cattttcatc tacagctgaa
    26041 gtaattttat tggttgcatt cttaacgtac tcactgttag atcccttgtc ttctacatct
    26101 ttaacgattt cgtccacttt cgcgttggtt agatcagaat cttcagtcgg taatacttga
    26161 tccgtaatat ttcctaacgt aaactcaaca tcaccataat ctgccttgtt gtatttcgtt
    26221 acacctgtag gatattgatc gtgcgtataa ccatcatttt tgattccgtc aatatcaacg
    26281 ttcaattgcg tactttgatc gtacattaat ttatgaaccg tgatattgac cctttctggt
    26341 ggcgtttttt gcgtgacatc ttctgcataa gctcgatacc cctgaaagca gcctgcaatg
    26401 attcctaaca ttccaacaat cattccagct ttttttacat accgatttat tgccattttt
    26461 tactctcctc atttcatatt tcaacgactc gcgttgatta aattcttgtt attgtagctc
    26521 tccctgtacc acgtatcttt cttttccttg tgacgtataa acacaagtaa acaacgttaa
    26581 taagggtttc tctcctttct ttggtaaaga caagaaagag ttctcttcag gaacgccatc
    26641 tattaattta aagtactcag gtacgataaa tttacgcgtt accttgtaac gatagacgcc
    26701 atcgttacta actaagtcca tcacctcccc taatgacaac ttttgaatat cagaaaataa
    26761 aacacctggc atttccatgt tatgacctgc taggacatag tttcctttac ccatttcggc
    26821 atcttcataa aaataagtag ccgctccaag actcaaagtg tattgatttg cccctttaaa
    26881 aattggcaat gctaaaccaa gtgcaggaat ctgaatttgt gcaaatactt gtttattgac
    26941 gcccatttcg attgcttctt tttgtatgga caacatttgc cccaaagaca tatcattatt
    27001 gacgttcgtt gcatcctcct tataactctt tgcgtgtgca ttgatttgat ctacgggcat
    27061 ctttttttct atattttgct taaacacctc tccttcatgt ttttggtagc taacatagcc
    27121 aaacacactg caaacgacga ttaaagcgac tcctaaaaaa ccaaataacc attttttcat
    27181 ccatctgcct cctttcagac aaaacaaaaa acactttttc acaacgaaaa agtgttttct
    27241 cttattacta ttctatttca tttatctggt ggttttatat tggtgtgtag ccattgctaa
    27301 cattaagaat acaattggca cccaactgat tgtcgttaat gtttcttcca tgaacgattg
    27361 acacatgatc gctaaaaaag cccacaagaa tgttagttgt ccatttgtca gctcacgttt
    27421 tcttacgagc aaccataaga aactaagcaa aagaataatc gatagaacaa aaccaatcaa
    27481 tccatagtga ttgacgagtg cgtgatacgt attatgatta cctagaatct gatttcctct
    27541 atggaaaaag aaaggtttca tccctacaaa tatttgtcct gttttttcgc ctaatgtttc
    27601 atagaatttg cgccaaattt cttctcgtcc tgtaaataag tgaactttgt ctgacattgc
    27661 agcaaaataa gaaatcggta aagctagaac cgaaactgct gcatacatcc aaaaggacaa
    27721 gtgtgtattt cgaaaaacct tcttaggtaa aatttctaag ataatgaaga taacaagcgc
    27781 atataatgct gctttcgatt ggcaaaccca aatcgttgcc ccagcaagaa tcaacgctgg
    27841 taatgatagc aatcgcatgt accattttcc aaatgaacta gcaaatcctg tgataagaag
    27901 ggctaatgtc attaaagaac tacctatcgt atttgtattg atccaaatat ctgataattt
    27961 gttcgatgga ttccaaatac tttgtccttc tggtactggt atctcagtaa atactcgata
    28021 aaccgttacg agtaaaaaga catagaatac tacaagatac aagctacgat caattacagt
    28081 aaatttcgtc cattttacaa tcaaaataat agcaataatt gtcggaacga tcagatcacc
    28141 atagaaagta tctcctcggt agcttctaaa aaaagaaaag aaaaggtaaa aaagaatgcc
    28201 tgctgcaatg aagaatagat cgtttctctt taccttgtac agattcaaaa gaaacaaaca
    28261 gcctacgaaa aacgtcacta actgtgcagc attatcataa ataaataata tcgtaggagg
    28321 atttaacccc accgtccata ttaaatatag aatggatacc aacagtaaca gaaaaatggc
    28381 actcatttta ttgatcaatt tttctacaag ttcttgaatg tgactcactt cccttattta
    28441 ttgatttttt ctacattgac taattcacac tggatcacat cgcacttttt catattattt
    28501 gcttgaaagg ctaacagtgt gagtagtggt tttttacctg cttttggcaa ggataaaaag
    28561 gaattgtttt ccttagattg aactacctgg ctgtaatcag aaatttttcc tcttttggag
    28621 acttcatact gatacttgta atgagcatcg attagatcaa tttcttctcc ttcagataaa
    28681 tcagtcatat cggataataa aacgcttggt gtatctacgt tataaccagc aagaatataa
    28741 tttccatttc ccatttcagt atctagataa taagtagaag ctactcctaa actaagcgtg
    28801 tcgctattcg ctccttgaaa aacagggaga ataaatcctg tagaagaaaa aacaagtttt
    28861 ccagcaattt cacctttgag atccttttct aatgcctgtt tttgattagt aatcagttgt
    28921 ctcttagaaa tatccgttaa actggttaaa ttatcttgat aggttttagc ctgttcctga
    28981 atatggattg tttttgttac accatctact ttttcagcga ttttgtcctt ttgataacct
    29041 tgataagcaa aataactaaa aagaagaaaa acaatgatta cgcccacgta aggattaaat
    29101 aaagttttat tgtttttatt catctatcta ccttctatat atgaattaat ttacctacat
    29161 ctaaccaaga taaaccatgt taaattttac tattcttttt attcaataac aatgccacat
    29221 cataattgat cattttcata tcataatcgt caagaaactt ctaataatag ttggcaattg
    29281 gaacctgttt ttacataagt tcgcctcaac tgttcaaagt ttttcattga acctaacaaa
    29341 caagctttct cttttcctgt tatcttcata tcattgatat attgcatgac agataatctt
    29401 ttaaggtgtt tttgtttcat catttttagc aacttttctt ccatttcttt cgacactttt
    29461 tttctgtttt cgatttcttc ataattcatg agttttccta cctttcttgt tttttgcaaa
    29521 aataaaaaag cctatgatca catacgctct acacttcagt cttgggttat ctttttcttt
    29581 cgtttaatta ctggccgtgc tggtttcaaa tccttgattc cattatgggt tctccaataa
    29641 tcgaattctt ttcgtaatct ttctttttcc atatctgttc ctttagacag ggatttgaat
    29701 acatctttag gtgaataacc acgagctaac agctgtacat accttccttt tgtcaaagta
    29761 tccatatcca tcgcctctaa aactggtcgc gccatctaat tttcctccta aataaattcc
    29821 ttactcaatg cctaaggtaa tactaccttt ttattattcg tcgtgactcc ttctaagcct
    29881 ttatctgtaa aatttattcc tccagttttt attcttcttt atccgctaca aattcaatga
    29941 tttcaacgct gacaatataa tcactcaaat atgaccaagt atcttcaaca gataattcgt
    30001 ctccattatc atccgtaagc gtcattttaa accattcatt accttcatca tcttcaggta
    30061 gcaagccgaa tccaattgga ttatcagtca atttttgatc gtataaatca tcctcgtcta
    30121 atgctaaaac atctaatcct ttacaatttc cgcctcttct aaacgtcaca aatcctttgt
    30181 aatcccaacg ttggaaagaa accttaatcg tatgtgtgcc caatcgtata ttcggatcat
    30241 aatttttcat ttgttgtaac ctcctcataa tcagttagta ctataaaggt atctttgttt
    30301 ttatccaaaa aatctaataa atcttttgta gaagatatct cttcatcaac ttcaccataa
    30361 taaatacctt cgataattgt tgctatatct gaaaaactaa tttttactgg acatttatcg
    30421 tctaccgcat tcaataccga caaaattgct tctttttctg caatctctcg tttgaatttc
    30481 tttggagcta atcgcatgac actcaagtca ttttcaagct tttcaatcca tgcagtttta
    30541 ctcattttcg caaatttttg aatcattcct ctaccacctt tgctatctac taaattagtt
    30601 ttacttcata acgaatatct ttatcgtttt tcgttcactc aaaaatatat aaaataaatc
    30661 gactcgttta tggcgtccga aacgagaaac cctagtagac agcggtaaat gctcgattga
    30721 tccaatgatc aacgtcctcg caagtatcta catgtactgc agcacacgac acaatttctt
    30781 gtgcgtcctc cccattacga gaagacctac tccttattcc atgagtttgc gtcaaagcct
    30841 agatccctta gtagctgctc atgattaatc taggcgctat tctaacttgt ctaacttcta
    30901 aattccctct tgtttactct gtgcatatta tttacacaaa ttcatttcac cactatccaa
    30961 acaagggcat ttactcgatt gaccaatttt ttacgttacc aatcgtaaaa acctaccccg
    31021 tcactccccg aagggaattg ttgatagatc aagtctactc cttatttttc tagtgattct
    31081 aagttccttt agaagtctcg acaatatttt caagcaagcg aaaaacgata aaatacaagt
    31141 tatgagaatg atcaattcag ctgattaaac gccccatccc ttttgcggta caacgcaaga
    31201 ctccattttt tcttcgacca atcagtacac cgttttggtc ataaacatgt ttgattgttg
    31261 gatatagctc ttttaaaggg ataggatctt ttaaaattat ttgtttttgt ttcccaatgt
    31321 aataaatcat ttcttctgag actttgtctt tctggattat atatttctta cactgcacaa
    31381 cggttttact ttgaccagtt gatagtttct tcgtgatctt ttctgtagcg gtacaccgtt
    31441 taccgtagtg acaaatatct tcgagactca aaattcctgt atatgacata aaataatcca
    31501 cccacttact ttaaatattt ttaacaagtt tttcttgtta tttttcaaaa atagagaatt
    31561 gattttcgtt catgacaaac aaaaaagagg gaacgcattc aaacatgcgt tccctctaat
    31621 ttcagcgttc gtcatacggc aaagagcccc acggacattt ttataacatt atgcttaggc
    31681 atagattcat cgtaatatca cgtattttta accttaacac agccaaaaaa agtggtatca
    31741 cttttgaata tctgttatac taaatccgtt acgggtaata ccgtaacact gaaaggtaaa
    31801 cgcattaagg ggtatagact taatgccacc tgtggacgtc gacgaaggtt accagccttc
    31861 cgagtctcac agggcctttc ttttttttgt attttcaatt gtccaaattt atcgtccaaa
    31921 agtgcagaac gaaaatcaat tcatcacaag catagcagac aattaaattt aaaacaacta
    31981 aaaatataca aaaaagtgta attgaatatt agcagcactg cctgaaaaga agtaatatca
    32041 ggcttttcag cttggggaaa taagtgaatt acctctcttc atgaattatt ataactcaaa
    32101 aaagcacata tgcaaacact agttccctat ttttttgagt gaaaaataac taaaatatct
    32161 aacgattatg aatttaaaac ctacgattgt gattgttttc ataaactttt aatctaaaaa
    32221 ctccggaata ctaactattt atttgtaata tttgtaataa aattaaaatc ataaaaaagt
    32281 tctaatttaa aattgatcat ttttatagta attgcttttt atcaactact ctaaccaatt
    32341 ttccgtacta actgttggta attcgttaaa tgtttctctt tgttcgtttg caagctttgc
    32401 cgcaatagct ttttcgacaa accaattttt tacataaacg aaaattagat tctgcaaatt
    32461 gtttactttt tcagtttttt gtttttgata agctttcagt aaagtcatat ataatctttt
    32521 gtcagcatca attccataag attccagttc ttcataaacg acaatagttt gctctaattc
    32581 ttcagctttt ttctttgcat tatgaatagt ttggacggtt tgttgagctt cactgtaact
    32641 tgaagaaaat gtttttatca agtttaatac attttctggt acaaatgtat ttatactgtc
    32701 ctcttgcatg atgtcgacga agttatctag caaaacttga ttttgtaact ggtcagtttc
    32761 agtgtctatt atgtgtctat tagtgtctat agaattagaa tagaatagat tgtctgcgat
    32821 tttcgcaatt acggttgagt ccttgatatg actggatttt gccgtatttg cgattttcgc
    32881 aattacggta gaatcattga tttttcgcgg ttttgccgta tttgcgattt tcgcaattac
    32941 ggtggaatca gtattgtctt cctcttccat gattttaaag acgtcttgat tcgtaacttc
    33001 tggcttgcct aagtacaata aattaggcat agattcaatc ttaccatcac gtttatttac
    33061 tcttcctctc ttctgtttca gaagacctgc tttttctaat tcttttttga tcttagatac
    33121 tttaccttcg cggcattgta acaatttcat taattcttca actgtgaata tgaagaatat
    33181 attatcatca gaatctaccc agttattttt gactgaataa tcaaatcgat ctttaagtag
    33241 catataagca atttttgcat catttgaaag atccatgtat ttttcgttag taaaaaatac
    33301 cttaggcatt tgataatatt tttcacgata aatttctttt gatttgtaat aattaaaatc
    33361 attcatagcc aatcctcttt ctgtaggata gcgttttgtt caaaactatg ttacaatgaa
    33421 ttaaacaaat atatctatcc actatatatt tttctactgc tgataagtcg ccaaactttg
    33481 ttaaagcagt agtctttttt tatctttttt tagtttacct taaaaaataa attaaagcaa
    33541 accgctctat ttcagggttt aagctcatgg taactatatc acgatattac aataacatta
    33601 tatcacgata tcgtaatatt acaatatgaa gataagcgac ttatcagctt tgatactact
    33661 gatttttata aaaaaaatat cacgatatta tgatatcgtg atattgaatg taaattaaat
    33721 tttattttgt ttctttgcta gtttatatcc ttcaatcaag tattcttcag gtatggattt
    33781 taatagcgca tcttctataa cgtctggttt acttttgtct gattcagcaa tagtcgtcca
    33841 atatttgagt gctgacatta gaattggatt tatacgaatg ctatctgctg ttcttacact
    33901 acttccttga tcttttatga ctttacttat atttttcttg gttaattcga tttcttctac
    33961 ttcttttggt ttgtagttct ttttattttt atttcctagt agtctactta ctgcagcttg
    34021 actgcgatta tcttctgcca tcaaaatcac caacttcttt taaatattct tctgtaaatg
    34081 ctttatagtc atcttcagct gttgagggtt tattaaaatt gattagatct gatccagcag
    34141 cttgcgcttc gtcaacggtt attgttctac gaatataagt tttgaaaatt tttgcaccaa
    34201 tgactctaga aatttcggtt gctgcagtcg tcatttcttt agaaatgtta taacgcttat
    34261 tatacttagt aaataggact cctcgtactt caagcttgtc gttcgtatat tcttttaccg
    34321 tttcaatagt ttcacttaac tttattagtc cttttaatga gaaatacgca gcttcaactg
    34381 gaatgataat ttcatcagaa acagtaaatg cgttgactgt aagcattcct aaacttggtg
    34441 gacaatcaat tataatataa tcatagttgt ccttgattgg ttgaagtact cttttaagcc
    34501 tgtgttcact tcctacttga gtaaattccc tctctgcaga agaaagggct atgtcggctg
    34561 gcaaaacatc aattccttta tagtccagaa tagcatctgt aatgtcagcc tcccctttta
    34621 atacttcata aattgtaggt tggtttgttc catctatatc gaaacctaca ttatctgtag
    34681 catttccctg aggatcaatg tccactaaaa gtacattttt accgtaaaca aatttcaaac
    34741 atgctgctaa attaacgcta gtcgtagttt tagaaactcc acctttttga ttggctactg
    34801 acaatattct tgccattaaa atatccccca ttcatcttat attataatat aattatatca
    34861 taatattgtg atattgcaat atcgtatatg cattatattg taatattgtg tgattatttt
    34921 gttttaaaga tatatatcgg ttcggatata aagattattc atatgttatt cgaaagtaaa
    34981 ataatcactt tttattactt taaaattttt tattaatcat aagaaatatg ttttgcgagg
    35041 agagtgtaaa atattttgtg taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DAJSVD010000020        34767 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSVD010000020 DAJSVD010000000
VERSION     DAJSVD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.020"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            160..840
                     /locus_tag="ON498_000829"
     CDS             160..840
                     /locus_tag="ON498_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3939123.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ON498_000830"
     CDS             930..1964
                     /locus_tag="ON498_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3939124.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ON498_000831"
     CDS             1978..2313
                     /locus_tag="ON498_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939125.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ON498_000832"
     CDS             2297..4777
                     /locus_tag="ON498_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939126.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ON498_000833"
     CDS             4833..6392
                     /locus_tag="ON498_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939127.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ON498_000834"
     CDS             6389..7753
                     /locus_tag="ON498_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3939128.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ON498_000835"
     CDS             7924..8994
                     /locus_tag="ON498_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939129.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ON498_000836"
     CDS             9009..9677
                     /locus_tag="ON498_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939130.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ON498_000837"
     CDS             9697..10275
                     /locus_tag="ON498_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3939131.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ON498_000838"
     CDS             10676..11587
                     /locus_tag="ON498_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3939132.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="ON498_000839"
     CDS             complement(11965..12882)
                     /locus_tag="ON498_000839"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR3939133.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ON498_000840"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ON498_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3939134.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ON498_000841"
     CDS             13579..14127
                     /locus_tag="ON498_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR3939135.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ON498_000842"
     CDS             14242..16602
                     /locus_tag="ON498_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3939136.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ON498_000843"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ON498_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3939137.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ON498_000844"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ON498_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3939138.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ON498_000845"
     CDS             19108..20007
                     /locus_tag="ON498_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939139.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ON498_000846"
     CDS             20000..21262
                     /locus_tag="ON498_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939140.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ON498_000847"
     CDS             21423..23321
                     /locus_tag="ON498_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR3939141.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ON498_000848"
     CDS             complement(23651..24370)
                     /locus_tag="ON498_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR3939142.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="ON498_000849"
     CDS             24707..25039
                     /locus_tag="ON498_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939143.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ON498_000850"
     CDS             25026..27029
                     /locus_tag="ON498_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR3939144.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ON498_000851"
     CDS             27048..27518
                     /locus_tag="ON498_000851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR3939145.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ON498_000852"
     CDS             27539..28123
                     /locus_tag="ON498_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR3939146.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ON498_000853"
     CDS             28138..29124
                     /locus_tag="ON498_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR3939147.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ON498_000854"
     CDS             29114..29425
                     /locus_tag="ON498_000854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR3939148.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ON498_000855"
     CDS             29447..31228
                     /locus_tag="ON498_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR3939149.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ON498_000856"
     CDS             31221..32597
                     /locus_tag="ON498_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR3939150.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ON498_000857"
     CDS             32604..33239
                     /locus_tag="ON498_000857"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR3939151.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ON498_000858"
     CDS             33269..34624
                     /locus_tag="ON498_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR3939152.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11490 a         5986 c         7455 g         9836 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaatatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctcttt
//
LOCUS       DAJSVD010000021        34749 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSVD010000021 DAJSVD010000000
VERSION     DAJSVD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.021"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1152
                     /locus_tag="ON498_000859"
     CDS             <1..1152
                     /locus_tag="ON498_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCR3939153.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ON498_000860"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ON498_000860"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3939154.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ON498_000861"
     CDS             2728..3003
                     /locus_tag="ON498_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR3939155.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ON498_000862"
     CDS             3009..3776
                     /locus_tag="ON498_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR3939156.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ON498_000863"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ON498_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR3939157.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ON498_000864"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ON498_000864"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR3939158.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ON498_000865"
     CDS             7920..8222
                     /locus_tag="ON498_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR3939159.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ON498_000866"
     CDS             8225..9388
                     /locus_tag="ON498_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3939160.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ON498_000867"
     CDS             9414..12842
                     /locus_tag="ON498_000867"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR3939161.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ON498_000868"
     CDS             12863..13987
                     /locus_tag="ON498_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939162.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="ON498_000869"
     CDS             13977..14414
                     /locus_tag="ON498_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3939163.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ON498_000870"
     CDS             14398..14736
                     /locus_tag="ON498_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR3939164.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ON498_000871"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ON498_000871"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR3939165.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ON498_000872"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ON498_000872"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3939166.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ON498_000873"
     CDS             15893..16936
                     /locus_tag="ON498_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR3939167.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ON498_000874"
     CDS             17095..17823
                     /locus_tag="ON498_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3939168.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ON498_000875"
     CDS             17939..18184
                     /locus_tag="ON498_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3939169.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ON498_000876"
     CDS             18181..18399
                     /locus_tag="ON498_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3939170.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ON498_000877"
     CDS             18535..19074
                     /locus_tag="ON498_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR3939171.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ON498_000878"
     CDS             19067..21340
                     /locus_tag="ON498_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR3939172.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ON498_000879"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ON498_000879"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3939173.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="ON498_000880"
     CDS             complement(22513..22767)
                     /locus_tag="ON498_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3939174.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ON498_000881"
     CDS             complement(23167..23436)
                     /locus_tag="ON498_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939175.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="ON498_000882"
     CDS             complement(23906..24361)
                     /locus_tag="ON498_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3939176.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ON498_000883"
     CDS             complement(24499..26427)
                     /locus_tag="ON498_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3939177.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ON498_000884"
     CDS             complement(26624..27457)
                     /locus_tag="ON498_000884"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3939178.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ON498_000885"
     CDS             complement(27649..28353)
                     /locus_tag="ON498_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3939179.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ON498_000886"
     CDS             complement(28376..29002)
                     /locus_tag="ON498_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3939180.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ON498_000887"
     CDS             29328..31703
                     /locus_tag="ON498_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR3939181.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ON498_000888"
     CDS             complement(31727..32218)
                     /locus_tag="ON498_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR3939182.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ON498_000889"
     CDS             32405..33325
                     /locus_tag="ON498_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3939183.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ON498_000890"
     CDS             33479..33823
                     /locus_tag="ON498_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR3939184.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ON498_000891"
     CDS             complement(34013..34513)
                     /locus_tag="ON498_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3939185.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT        11489 a         6079 c         7157 g        10024 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatc
//
LOCUS       DAJSVD010000022        33853 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSVD010000022 DAJSVD010000000
VERSION     DAJSVD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.022"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(247..1974)
                     /locus_tag="ON498_000892"
     CDS             complement(247..1974)
                     /locus_tag="ON498_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR3939186.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1967..3160)
                     /locus_tag="ON498_000893"
     CDS             complement(1967..3160)
                     /locus_tag="ON498_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3939187.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3347..3991)
                     /locus_tag="ON498_000894"
     CDS             complement(3347..3991)
                     /locus_tag="ON498_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3939188.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4085..4219)
                     /locus_tag="ON498_000895"
     CDS             complement(4085..4219)
                     /locus_tag="ON498_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR3939189.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="ON498_000896"
     CDS             complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="ON498_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR3939190.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6350..6823)
                     /locus_tag="ON498_000897"
     CDS             complement(6350..6823)
                     /locus_tag="ON498_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR3939191.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7224..7448
                     /gene="nrdH"
                     /locus_tag="ON498_000898"
     CDS             7224..7448
                     /gene="nrdH"
                     /locus_tag="ON498_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3939192.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7607..9766
                     /gene="nrdE"
                     /locus_tag="ON498_000899"
     CDS             7607..9766
                     /gene="nrdE"
                     /locus_tag="ON498_000899"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3939193.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9816..10781
                     /gene="nrdF"
                     /locus_tag="ON498_000900"
     CDS             9816..10781
                     /gene="nrdF"
                     /locus_tag="ON498_000900"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3939194.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10870..12009)
                     /locus_tag="ON498_000901"
     CDS             complement(10870..12009)
                     /locus_tag="ON498_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3939195.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12318..13031)
                     /locus_tag="ON498_000902"
     CDS             complement(12318..13031)
                     /locus_tag="ON498_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3939196.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="ON498_000903"
     CDS             complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="ON498_000903"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3939197.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14220..15752
                     /locus_tag="ON498_000904"
     CDS             14220..15752
                     /locus_tag="ON498_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3939198.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15943..17145)
                     /locus_tag="ON498_000905"
     CDS             complement(15943..17145)
                     /locus_tag="ON498_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939199.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17227..17916)
                     /locus_tag="ON498_000906"
     CDS             complement(17227..17916)
                     /locus_tag="ON498_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3939200.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIHSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18022..18390)
                     /gene="rplL"
                     /locus_tag="ON498_000907"
     CDS             complement(18022..18390)
                     /gene="rplL"
                     /locus_tag="ON498_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3939201.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18443..18946)
                     /locus_tag="ON498_000908"
     CDS             complement(18443..18946)
                     /locus_tag="ON498_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3939202.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19150..19277)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19356..20045)
                     /gene="rplA"
                     /locus_tag="ON498_000909"
     CDS             complement(19356..20045)
                     /gene="rplA"
                     /locus_tag="ON498_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3939203.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20149..20571)
                     /gene="rplK"
                     /locus_tag="ON498_000910"
     CDS             complement(20149..20571)
                     /gene="rplK"
                     /locus_tag="ON498_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3939204.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20914..21582
                     /gene="sdaAB"
                     /locus_tag="ON498_000911"
     CDS             20914..21582
                     /gene="sdaAB"
                     /locus_tag="ON498_000911"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR3939205.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21595..22467
                     /gene="sdaAA"
                     /locus_tag="ON498_000912"
     CDS             21595..22467
                     /gene="sdaAA"
                     /locus_tag="ON498_000912"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR3939206.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22558..23577)
                     /locus_tag="ON498_000913"
     CDS             complement(22558..23577)
                     /locus_tag="ON498_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3939207.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23750..24655)
                     /locus_tag="ON498_000914"
     CDS             complement(23750..24655)
                     /locus_tag="ON498_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3939208.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24714..25259)
                     /gene="nusG"
                     /locus_tag="ON498_000915"
     CDS             complement(24714..25259)
                     /gene="nusG"
                     /locus_tag="ON498_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3939209.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25360..25530)
                     /gene="secE"
                     /locus_tag="ON498_000916"
     CDS             complement(25360..25530)
                     /gene="secE"
                     /locus_tag="ON498_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3939210.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25554..25706)
                     /gene="rpmG"
                     /locus_tag="ON498_000917"
     CDS             complement(25554..25706)
                     /gene="rpmG"
                     /locus_tag="ON498_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3939211.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25868..26512
                     /locus_tag="ON498_000918"
     CDS             25868..26512
                     /locus_tag="ON498_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3939212.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26568..27071)
                     /locus_tag="ON498_000919"
     CDS             complement(26568..27071)
                     /locus_tag="ON498_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3939213.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27084..27130)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27668..28633
                     /locus_tag="ON498_000920"
     CDS             27668..28633
                     /locus_tag="ON498_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3939214.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28732..29904)
                     /locus_tag="ON498_000921"
     CDS             complement(28732..29904)
                     /locus_tag="ON498_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939215.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29891..31036)
                     /locus_tag="ON498_000922"
     CDS             complement(29891..31036)
                     /locus_tag="ON498_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939216.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31050..31974)
                     /locus_tag="ON498_000923"
                     /pseudo
     CDS             complement(31050..31974)
                     /locus_tag="ON498_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32091..33170
                     /locus_tag="ON498_000924"
     CDS             32091..33170
                     /locus_tag="ON498_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3939217.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33202..33795
                     /locus_tag="ON498_000925"
     CDS             33202..33795
                     /locus_tag="ON498_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939218.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT        10307 a         7026 c         6023 g        10497 t
ORIGIN      
        1 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
       61 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttgg
      121 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
      181 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
      241 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
      301 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
      361 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
      421 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
      481 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
      541 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
      601 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
      661 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
      721 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
      781 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
      841 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
      901 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
      961 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
     1021 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
     1081 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
     1141 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
     1201 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
     1261 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
     1321 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
     1381 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
     1441 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
     1501 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
     1561 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
     1621 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
     1681 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
     1741 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
     1801 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
     1861 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
     1921 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
     1981 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
     2041 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
     2101 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
     2161 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
     2221 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
     2281 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
     2341 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
     2401 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
     2461 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
     2521 gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
     2581 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
     2641 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
     2701 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
     2761 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
     2821 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
     2881 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
     2941 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
     3001 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
     3061 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
     3121 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
     3181 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
     3241 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
     3301 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
     3361 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
     3421 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
     3481 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
     3541 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
     3601 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
     3661 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
     3721 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
     3781 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
     3841 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
     3901 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
     3961 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
     4021 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
     4081 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
     4141 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
     4201 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
     4261 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
     4321 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
     4381 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
     4441 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
     4501 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
     4561 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
     4621 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
     4681 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
     4741 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
     4801 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
     4861 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
     4921 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
     4981 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
     5041 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
     5101 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
     5161 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
     5221 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
     5281 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
     5341 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
     5401 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
     5461 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
     5521 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
     5581 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
     5641 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
     5701 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
     5761 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
     5821 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
     5881 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
     5941 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt
     6001 ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc
     6061 gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca
     6121 ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa
     6181 gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct
     6241 tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga
     6301 ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt
     6361 cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc
     6421 gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact atttttacat
     6481 ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta
     6541 cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag
     6601 cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac
     6661 tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg
     6721 caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga
     6781 cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt
     6841 gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca
     6901 aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact
     6961 tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt
     7021 catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt
     7081 tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt
     7141 agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata gataataaga
     7201 atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc
     7261 aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata
     7321 tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg
     7381 tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg
     7441 ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag
     7501 caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat
     7561 ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa
     7621 tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca
     7681 aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc
     7741 aaatacgatg acttttctat ctattatgga aaaaatcaat tacttgatcg atcaagaata
     7801 tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt
     7861 tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca
     7921 atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc
     7981 cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga
     8041 aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg
     8101 tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg
     8161 gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt
     8221 atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt
     8281 cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg
     8341 tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc
     8401 agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat
     8461 cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc
     8521 atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta
     8581 tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga
     8641 aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac
     8701 agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat
     8761 tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac
     8821 ggatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt
     8881 tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat
     8941 cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc
     9001 catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt
     9061 agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa
     9121 tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg
     9181 cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc
     9241 attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt
     9301 aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc
     9361 atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca
     9421 agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc
     9481 gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaatggc
     9541 acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga
     9601 aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag
     9661 acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga
     9721 tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat
     9781 tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa
     9841 taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc
     9901 aattttggct agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt
     9961 ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg
    10021 tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag
    10081 ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt
    10141 ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc
    10201 cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag
    10261 aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc
    10321 ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca
    10381 tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg
    10441 agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc
    10501 tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag
    10561 aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc
    10621 cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag
    10681 gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag
    10741 aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa
    10801 aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct
    10861 tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac
    10921 atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac
    10981 tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat
    11041 caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt
    11101 gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg
    11161 aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac
    11221 atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc
    11281 gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact
    11341 gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg
    11401 aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat
    11461 aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat
    11521 gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga
    11581 taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc
    11641 caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg
    11701 aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat
    11761 tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa
    11821 aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaac
    11881 tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac
    11941 gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg
    12001 tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag
    12061 ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga
    12121 gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc
    12181 cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg
    12241 aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt
    12301 ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg
    12361 tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta
    12421 tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg
    12481 attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg
    12541 ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg
    12601 agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg
    12661 gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat
    12721 tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca
    12781 atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct
    12841 cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa
    12901 actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt
    12961 gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc
    13021 atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa
    13081 aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct
    13141 ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa
    13201 tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca
    13261 acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac
    13321 aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc
    13381 aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt
    13441 acttttcata attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt
    13501 atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt
    13561 tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa
    13621 gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc
    13681 cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa
    13741 ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc
    13801 atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata
    13861 taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc
    13921 ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat
    13981 ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct
    14041 ttttacaata attagtatag accaaattgc atttttttca ttttcttgac aataaatagg
    14101 aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa
    14161 gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta
    14221 tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag
    14281 gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg
    14341 gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc
    14401 attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat
    14461 taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa
    14521 ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg
    14581 ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg
    14641 agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa
    14701 gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc
    14761 tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt
    14821 ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc
    14881 agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc
    14941 taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg
    15001 ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc
    15061 gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc
    15121 cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag
    15181 cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt
    15241 tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta
    15301 ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag
    15361 ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac
    15421 cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac
    15481 tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg
    15541 gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc
    15601 tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac
    15661 agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg
    15721 tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg
    15781 agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg
    15841 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc
    15901 ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag
    15961 atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag
    16021 cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga
    16081 accagccgca cctccaataa agtacataaa cataaataca gtattatttc ggttgcttgc
    16141 tgcttcacct agcccttgta ctcgtgtttg attggcgact tgaccaaatt gattcccgac
    16201 gtctaataaa ataatagcta aaagcaaaat ggccaaatgg cttccgccaa ccaataaagc
    16261 aataaagctt attgtttgca taacaagtcc catatacacg atttttcttt cagaataaga
    16321 atctgccagt cgtcccacga taggtgcagc aactgctcca gacaaaccaa aaatcgctaa
    16381 tatccctgct tcgaatgttc cccaattgta ggcgggactg ctgatataaa aaatcaacgt
    16441 agaccaaaag atcgaaaatg ttccaaacat gaaaaatcca ctgactgccg cttctcttaa
    16501 tagacgttga gaagcaacta aatgcggcaa acttttaagt gaatcaaggt atgaaacagc
    16561 tgtactactt tgctggacag aacgcggcat tttccagtaa agcagaagtg taagaattcc
    16621 gactgcacat aaagcaaata aatagatcat ccgccaagaa aaaagactgg caacaatacc
    16681 agataccgtc cgagaaagca aaatccccac gagtaaaccg ctaagcaatg tacccatgac
    16741 cttccctctt gcttttggtc ccgctagtac cgcaccgtaa ggaatgatga tttgcggcac
    16801 gatcgaaagt aatcctatta aaaaagaagc gcatgcaaat aacgtaaacg aaggcgcaaa
    16861 aaaagcggcc aacagagaga cggcagataa cgctgccatt cgaatgatca attttggacg
    16921 atccaccacg tcccctaaag gaactaaaaa cagtaaccct aacgcatagc ctaattgggt
    16981 caacatcgtc aatatcccga ctgcacttgt gctaacagat aaactagctg ctacttttgt
    17041 tccaatcggc tggatgtaat acatattggc aacaacaacg ccacaagtga ttgataatag
    17101 taaaataagc gaagttgtta aaggtttttt ttcattactc ttcattgatt tcccccattt
    17161 cctatcttag tttacaactt atggaaaaga ttggaaatag aaaaatacaa ctattcgcca
    17221 cacttattat ttgtgctttt gttttccttg ttttttcttg cgatctttat aaatgaagac
    17281 aactaaccct actagcacag taacacttgc aaccaacgtg actgcagttt tgttatatcc
    17341 gtgaaacaga gcttctccac cacacgcata caacacagac gaagggatga ctccaatcac
    17401 gatcgacaat atgatttgct gtagaggcaa tttcatcgca tctgcagcaa aattgaccat
    17461 agaagatgga ataaccggta tcatatagcc taacattaca cctatttttg gatgtttcat
    17521 ttgtcggatt gcttgcaccc aatgattcgt ttcggttttc ttatctaaaa attttaagcg
    17581 atgcataagg aaaattgcag tgatgttacc aagagcatta cccaaaatgt tgatgatcgc
    17641 acctgtcagt ggtccaaagc tcaaaccagc aatgactcca actactgaag taggaatacc
    17701 tggaacagca cacatcaccg ctgtcaatac gatcaagagt atcgatgcaa aagtcccatg
    17761 cgaatgaata tgtgcgagta gctctgcctt actcgctctc ggatttaaaa ataattggat
    17821 gtctgtctga tattctaaat acaaccgata gcccactacc agtatcagaa tgattcccaa
    17881 cgagatcaat atcaatcgga ttattttctt tttcatattt cctcctgaaa aaaatctata
    17941 ttcattaaat aatagtttaa cataaaaaca aaaaagcacc gaaatccaac ataggatctc
    18001 agtgctttta aatagtaaaa attatttaac agttacagat gcgccaactt cttcaagttt
    18061 agctttcaat tcttcagctt cttctttaga tacgccttct ttaactggtg caggagcgcc
    18121 atcaactaca gctttagctt ctttcaagcc taaaccagtt gcttcacgaa ctgctttgat
    18181 aactttaact ttttggtctc cagctgcagt taattctaca gtgaattctg tttgttcttc
    18241 agcagcagca gcgccaccag cagcagctac agctacagga gcagcagcag atacgccaaa
    18301 ttcttcttca atagctttaa ctaggtcgtt aagttctagg atagttgctt cttttaactc
    18361 agcaacgatg ttttcaatgt tcaatgccat tataatttcc tccgtttaat tgttcttttt
    18421 tatttaatgc tttatgcggc aattaagctg catctccatc ttctttgtct gccactgctt
    18481 tgacagcgta agccacgttg cggactggcg cttgtagtac agatagcaac atagatagaa
    18541 gtccttcgcg atttggcaat tttgccaatg ctgtgatttc ttccaatgaa gagactttac
    18601 cttcgatgat accgcctttg atttctaatg cttttgcttc tttagcaaat tcgtccatga
    18661 ttttcgctgg tgcgactaca tcttcgttac tgaatgcaac agctgtaggt ccagtgaata
    18721 cttcgtccat gccttcaagg ccagcttttt tagctgcacg tgaaagaata gaatttttga
    18781 taactttcat ttctacgtta gcatcacgaa gttgtttacg taaacgtgtt acttcatcaa
    18841 ctgttaatcc gcggtagtca acgactacta ctgatgcagc tgctgtaaat ttttcgctta
    18901 cttcatcaac gattgctgct ttttttgcga taattgcttc actcattttt gagtttcacc
    18961 tcctgaattt tgatggaaga gttttttgca ctgaaaaggc aagtcacttt tatagttact
    19021 ttgctcaacc agtattattc atcacctatt cacttcgttc atagtgattc tactcgcttt
    19081 gctcgaaagg aaactccttt cgctttgctc aaccagtatt gttcatcatc tcttcacttc
    19141 gttcatagtg attcacaata aaaaactcta tgccaccgca gacatagagg gactattgaa
    19201 tatattcaat aaacgtcctc ggtaggaaat taaggcttga tgccacctac tgtcttcggt
    19261 acaagttaat tattaacctc aactagctta gcatgctaaa ctagttgagt caagaattat
    19321 ttttcagttg gatcaatcaa cattctaata tttaattaga aagaagcttg gtcaacatgg
    19381 ataccaggtc caaatgttgt tgtgatcgtt acatttttca tgtattgacc tttagctgct
    19441 gatggtttag cttttagcaa tacatcatgg attgctgcaa agttttcaac taatttttca
    19501 tcgtcaaatg aaacttttcc gattggtaca tggatatttc cagctttgtc cacacggtaa
    19561 gttactttac cagctttcac ttcgtttact gcttttgtta cgtccattgt aactgtacca
    19621 gttttagggt ttggcattaa gccttttggt cctaatactc gtcctagacg acctacagta
    19681 gccatcatat caggtgttgc aacaactaca tcaaagtcaa accatccgtt ttggattttt
    19741 tgaaccatgt cgtcgtcacc tacgaaatct gcaccagctg cttcagcttc ttttgctttg
    19801 tctcctttag caaaaactaa aacagtttgt gttttaccag ttccgtttgg taatacaaca
    19861 gcaccacgga tttgttggtc tgctttttta gggtctacgt tcaaacggta tgcaacttca
    19921 actgttgcat caaattttgc aatgtttgtt tcttttgcta aagctactgc ttcggcaaca
    19981 ggataaactt tacttgaatc aacttttttt aatgcatctt gcattttttt gctcttttta
    20041 gccattttgg ttcctccttg attgtggtta taacggttgt acctcccacg tgttctatat
    20101 ctttcaatat agagccaact gagaagctgc tgtcttaggg tgtatagatt attctacagt
    20161 gatccccatg cttcgtgcag ttccttcaac catgcgcatt gctgcttcaa cgttagctgc
    20221 gtttaggtct tccattttta attcagcgat ttctcttact tgatcgctag aaactttcgc
    20281 aactttattt ttgtttggtt cgcctgaacc tttttcgatt ttagctgctt ttttcaatag
    20341 aacagcagct ggcggtgttt ttgtaacgaa tgtgaatgaa cggtcttcgt atacagaaat
    20401 aacaaccgga atgatcaaac ctgcttgatc agctgtacga gcgttgaatt cttttgtgaa
    20461 tcccatgata ttgatacccg cttgacctag tgcaggacct actggtggag ctggagttgc
    20521 tttacctgca ggaatttgca atttaacgat tttttctact ttctttgcca cgagacatac
    20581 ctccttaagt ccgtgatgtg gttaattgga gatgcatatt tctcctccca cgttttatct
    20641 gatctgaaaa aatccagaca aataatgtgc aaaacaatgc acactggtac agtatagcaa
    20701 atttatttgt tttttcaaga tttttttctg ctatttttac attttgcttg aataaacgct
    20761 aaatttattc taaaaaaaca acaatttttc gttctcattt cttcttttat gttacacttg
    20821 cgctagcatg gttcaacaga tgaaagcaaa caataacacg gttttttaga cccttgtaca
    20881 aagattccta cataacaaaa tgaggtggag aaaatgaaag atttaaggta tagaagtgtc
    20941 tttgatatta ttggacctgt gatgattggt ccaagtagct ctcacactgc cggagctgct
    21001 agaatcggga aaatcatacg tagtatattt ggcgaacagc ctgattctgt agatatttat
    21061 ctatatgaat catttgcaaa aacctatcgg gggcacggaa cagatattgc attagttggc
    21121 ggcctgttag ggatggaacc cgatgatgaa cgtttagctg attctttgaa attggcatat
    21181 gaagccggta tggaagtctt gtttgttcct aagagtgaaa aagccgaaca tccaaactct
    21241 gtaaagatgc ttctaaaaaa aggaacacga aagctttcag tcactggcat ttcgatcggc
    21301 ggtggcaata tccagatttc tgaattgaat ggattcaaaa tttctttaag catgggtact
    21361 cctaccttga tcgttgtcca ccaagatgtt ccaggtatga ttgctaaagt gactagtata
    21421 ctttctgaga ccaatacgaa tatcggcact atgacagtga cacgggaatc aaaaggtgaa
    21481 aatgccatta tgataattga agtagatgat cctcaagtag aagaaacggt aaaaagactc
    21541 aaacagctgc caaatatcga cagcgtaaat tatttcgatt aaggagctaa aaaaatgttt
    21601 ttatcgattg aagaattagt agatcaagca aaagcttatc caagtgtagc agaattgatg
    21661 attgcagttg aaatggaaat gagtggccgt tcccgtagtc acatcatcga gatcatggaa
    21721 aaaaacctct cagtaatgga acaatcaatc aacgagggaa aagaaggtgt tacctccgtc
    21781 acaggaatta ccggtggtga tgcaaaaaaa ttagatgatt atttaaatga ggcagacttt
    21841 ttaagtggag aaacaatctt aaaagctgta cgcaatgctg tagctgttaa tgaagtcaat
    21901 gcaaaaatgg ggttgatttg tgctacaccg actgccggaa gcgcgggtgt tgttcctggt
    21961 gtgctgatgg ctgtccgaga tcggcttgat ttgacacatg aacagcaatt ggaattctta
    22021 tttaccgctg gcgctttcgg gttagtcatc gcaaacaacg cttctatcag tggtgcagaa
    22081 gggggatgtc aagcagaagt tggttctgca agtgcaatgg cagcagcagc tttggtttgt
    22141 gcaaaaaatg ggactcctca tcaagcagca caagcagtag ccattacatt gaaaaatatg
    22201 atgggactga tttgcgatcc cgttgcaggc ttagttgaag tcccgtgtgt caaacgtaat
    22261 gctttaggct cttctcaagc ttttatctct gctgatatgg cattagcggg tatcgaaagt
    22321 gtcattccgc cggatgaagt cgttgctgca atgtaccaag tcggacgtca aatgccgtcg
    22381 attttcaaag aaacagcaga aggtggctta gctgttacgc caactgctaa acgacttgcg
    22441 gaagagattt taaataagag ggcgtgataa agctccttaa tttcgagtat taagagatta
    22501 gttatcttca taacaaaaaa cgaagagagg actaattgcc cttctcttcg ttttttatta
    22561 atccatcaat ttcgtatttt ttacttcatc gatcgttttt gttcctgcaa gttgcatcgt
    22621 gatcgacaat tctttgttca ggtgttcaaa aacagatgtt actccttctg ccccacctag
    22681 attcaaccca taaatcactg gtcggccaat cgcaactaag tcagcaccac ttgccagagc
    22741 tttgaagaca tgctctcctc gacggacacc agagtcgaaa atcactggta cacgtttgtt
    22801 gactacttcg gcaatttttg gcaatacttc aaaggacgca ggacctccat ctagttgacg
    22861 tccaccatga ttggataccc agataccatc tgcccctgca gagatagcga ttactgcatc
    22921 ttctggcgat tgtattcctt ttacgataac tggaagatgg gtgatttctt tgattgtttt
    22981 gatgtcatct ggtgtcaagc cttgttttgc agctgcataa atttctgcga ttccttttcc
    23041 ttcgccatta ccacttgcac tttgttcgct atacgcagct aagttaggca ttggtaatgg
    23101 aaattggaat tgattgatga cgtcttcttc acggtaacca ccgagcgttg aatctgccgt
    23161 taaaatgata gctttagccc ctgcttctac tgctttgttc aaaatgaact cattgaatct
    23221 gtcgtcttta ctcatatata attggaaaaa ctgcggtgcg cctggcgctg catcggctgc
    23281 gtctttgatt gtcgtatttg cataagtgct gatggaaaag attgatccag cagcagctac
    23341 tcctttagct gtatcagctt cccctttttc atgtgccaat ccttgggccg ctgatggcgc
    23401 ttggataatc ggtgtatcta attcaattcc gaaaacagat gtatgcaaat cggctgagtc
    23461 gattccgcgt aatatccgtg gcatgatttt ctttgtatta aaagaagtcg tattttcttt
    23521 catcgtccac tcatcttcag aaccaccgcg aatatagcca aaagctcctg cttccatgcg
    23581 ctcttttaca cgaccttcta gttaggcaat gtttacaata tcgattggat gttcttcatt
    23641 acttgtatga taagtcatag tagatccttc tttcatcttc ttgattacat ataaatcata
    23701 ctaaacttac aaaaagtaag caaacattta ctctcagata cttttatcgc tattctttcc
    23761 aaataaatcg acttacaagc tgatctactt cttggttttc atgaagcata ctatgagagg
    23821 cattttcacc tgtcactact ttttcctcta tttcatttcc gcgttgcttc aacaacgcat
    23881 agaccgctaa tgcactgttt aacggtactg ttccatcact caagtctgtt tcatctagtt
    23941 gaccagcaat caagaaaaat cgagtggtta taggaatatt accgatcatt tgctggtaat
    24001 cttgatagcg gttagaaacg acagcaggcc cctttgctaa ctcatctgtc agagactgcg
    24061 cactatcatc gacaaaatca ttaaaaggcg cgccaattgc tgttaatgtc tgaacttgtg
    24121 gcaatgacgt atcctgtcca tacgttccta aatagcgtag tgcactaact cctcccatgg
    24181 aatgccccac tatgttgact ttatctatgc cgtagttttc tttcagatac aaaagacaag
    24241 ttttgatcca ttctgcttga ttccactcgt tatttttatt atcttcaaac aatacttgga
    24301 ccatcggatt gtctgctttt ccagacaaat ctcctgttgc ttgtatttgt ccatctgttc
    24361 ctactgttaa aaccaattct tttactgctt gctgattttt ttccaaacgc cgcaacatcc
    24421 ctttaaagga attttctgtc ccgctgtagc cgtggaaaaa aagtgttgga atagtggttt
    24481 tttctatcac tttactggat tctgccgttt tttctttatc agaaacttca gtagtattta
    24541 tcttcgtcgt ctggcaacca cttaacgcaa acaaactgat aaggactaaa gctgctgcag
    24601 ttgcacgttt gcataaattt ttgattggtt ctgtcataca cctcttcctt tccatataga
    24661 aaaaagaaag acttgaaagg ccaatcatat gatcgacatt tcaagtctct ttattaatcg
    24721 attttatcta cttgatcgaa atcaagttct gtactggttt cacgtccaaa catatcgatg
    24781 ttgactttca atttttcttt ttctccgtca acttctgtta cttgtccttc taatccagca
    24841 aatgcgcctt cgatgatttt cacgacttcg ccaacttcta cttctagatc attttgtcgc
    24901 aggctcatac cgatagaacg caagatatga ttgacttctt ctggaagtaa tggtgctggt
    24961 ttgcttccgg ctccgtgtga accaacgaat cctgtcacac caggtgtgtt gcggacaaca
    25021 taccatgaag cgtctgtcat gatcatttca acaagtacgt atcctgggaa tgttttatga
    25081 acgatttctt tttctttccc atttttgact tcagtttctt tttcttcagg aacaacgaca
    25141 cggaaaataa agtcggccat tttcatactt tgcgcacgag attcgatatt tgcttttact
    25201 ttgttttcat atcctgaata tgtatgcaag acataccagt ttttttcaaa agattccatt
    25261 gaaggctcct catttcattg taaaataaaa aaacctcagt acacccgaag tttttctttc
    25321 gcttcattat agcactaaaa gtacgttgtg gctagcgagt tattttaaaa tccagccaaa
    25381 taagctttgg atggctgtat ccattacgta aaacatcaat ccgaaaataa tagaagtttc
    25441 gatgactact aacgtatctt tacggagttg ttttctgctt ggccaagtta cttgtttgat
    25501 ttcatctttt acgcttttta gaaatttcat gagacaacct cccttgctac tgtttatttc
    25561 gtttctttat gtaatgtata ttgattacaa tatttacaaa acttattgat ttccaaacgt
    25621 tcgccgcttt ttccttcact gactgatttt gtgtagttgc gagacccaca aacggaacaa
    25681 gcgagtgctg cttttttctt tgccatttac ttctgccccc ttgctgagaa tcagacttat
    25741 acttcataac agtatcacgt aactttttac ttgtcaattt gtgaaataaa ctatctattg
    25801 ttttggataa aattatgata tgattaattt gaaattaagc taaaatgaga caattggagg
    25861 tatagcgatg ctattaaaaa cacttgtata taagaaaaaa gatctgacaa ccgttacaga
    25921 gactagtaca ttagaagaag cattaaaaat tttagaaact tccggatacc gttgtgtgcc
    25981 gatcttagat tcttcaggta atattttccg tggaaacatt tacaaaatgc atatctatcg
    26041 tcataaagca aatggcggtg atatgacgct tcctgtcact catcttttga aaaacgctac
    26101 aaagttcatt tacctggata cttctttctt caaagtattc tttacgatca aagagcttcc
    26161 ttatattgct gtccttgaca gtgaaaatca tttttatggt attttgacac atagtacgtt
    26221 gcttaatatg ctctctcaat cttggagcgt agaacaagga agctatgtac tgacaattgc
    26281 ttccacagga aagcaaggag atttggctgc tattgcaaaa atcatttcaa aacacagcag
    26341 cattgccagc tgtattactc ttgatatcga aaaagacgaa tacattcgac gtacattgat
    26401 cacgctaccg gctaacactg aagcgaaagt atgtgaaacg atcgtcaacc acttagagaa
    26461 aaagaattat aaagtggtag aaattgaaaa tttgcaaaac caagaagcat aatcaaaaag
    26521 cgaaaaaaga aaacagctaa tctgctttct tttttcgctt tttataatta gacacgcttt
    26581 tcaaaatcaa gcgcgcgatc taaataatac atcaatggtg ctgaaacagc catgacgata
    26641 aactcactta atgcaagcgt gccataggtt gtccaaaatg cttggccacc tgttggaact
    26701 aacatataag caatcagtcc catgctgatg gtaaagaaga tcgtgttcaa tactaaacgc
    26761 atctttacat ttttcacttt gttttgcaat aaagctgtca ggcctaaaga aagcaatgtt
    26821 tggccgccac caaaaaccac atccatgata ccaaaaccaa agaaggcatt atagacgatg
    26881 accccgccta aaactcccca aagcaattta cggttaaaga caactaaatg attcaaactt
    26941 tctgaaatac gaaactggat cggccctgag gatacaggcg caactaaaat agttaacgct
    27001 agataaagag ccatgatgat gccatttaca acgattacgg ataaccgttg tctgcttttc
    27061 gttttttgca ttttctctcc tcttttctcc tagttttttt aacgcgggat ggttgatgaa
    27121 ccgcggttat gtgaaacaaa caataattat aacagacttt tcatttattt caatccgtct
    27181 tatctgcttt taaaaaagca aaagcttaaa caatgtaaac gtttgctaaa aatcgtggga
    27241 tatggtataa agtaagtgta aggggtcata agaattccca gtaaagcaga cgaagtagga
    27301 tctgcaagaa cagaattgtt gtgaaactta aataaataaa aaaggatgtg ggaatttgag
    27361 caacctctta ctagagaacg gtctagcaag cagctgaaga tctaagtgga catgccgaac
    27421 cttggaactt ctccttacgt aagtaatgga tcatgcgctt gctaggcttt tttgtgtttt
    27481 tcggtcttcg gacctttttc attttcctag acttctgcta taataaccaa gaattaattt
    27541 tgtaacagat ttttttgaaa aaacaagttt ctttcattca aaaaatagaa aaagcttgct
    27601 aattattggt taaccagcaa acaggagtat taaaatatct tgttttattg aatagcagga
    27661 ggaaatcatg aaattccatt atcatatatt gatcaatcca tcggcaggca gcggaaatgg
    27721 ctataaagta gcagaaagaa ttttaccggt attaaaaaat aagcatattg actatacact
    27781 tcattatagt gaatacaaag gacacgatgc tgagattgct gaaactttgg caaaagaaac
    27841 attgattcct tggatagaag aagaacagaa aacaatcgat actttccccc ttttgatcat
    27901 cgtaggaggt gacggcacct tacatcaggt acttgatact ttctatcaga tggaagtaga
    27961 attcccagtt gcttatattc cagcaggttc tggagatgat tttgctcgag gggcaggcct
    28021 tccaaaaaat cccaaaaaag gacttcagct gattttagca gctcaatcac cagaaaaagt
    28081 ccacataatg gcttatgaag aaaaaataag tgaaaaaaaa ggaatagctg ttaataattt
    28141 cggtatagga ttagatgcag ctatcgtcca tgcgacaaat cattcttcca caaaaaagcg
    28201 attgaacaaa tacaacttag gatcattttc gtatttattc tctattctcc gtgctttatt
    28261 cactcaaaaa ggatttccaa ttctggtaga tgcaggagga aaacaattaa gtttttcaaa
    28321 cgcattttta tgtacagcaa cgaagcatcc tttttttggc ggaggaatcg cgattgttcc
    28381 aactgctgat gcgtctaaac cagtcattga ttttgtcgtg gtcgaacgaa tcaatttctt
    28441 taaaatcttg tggctcattc ttttacttat acagaaaaaa caattgaaat cgaaatattt
    28501 ccatcactac acaacaaaca gattacgtat cgtttcaaca acaccgcaac atggtcaaga
    28561 agatggagaa gaaatggaaa aacgaccttt tgatatcaca ataaccacaa ctaaacagtt
    28621 gttttggtgt taataaaaga ggttttgtca gaagctacat ctggaacacc gtttattcgg
    28681 aaaaaaggac tgtgacacga cttttgtcac agtccttttt ttactatgca gttaattctt
    28741 tgcccaattg ggttgccatc gatagtttct ttctttaccc aaaataaatt ggatcattga
    28801 aaacaccgca gcaaaagcaa ctaagacaca accattgaac caaaaagatt ctgcaaatgt
    28861 ttgattgtaa ctgagtgttt cactgatcaa gttattattt tgaacaaacc aatatgttgg
    28921 tgtgaatgcc gctattttgc taaccatttc tggcaagtat tctgctggta caaacacccc
    28981 accgataaag caactgcctg tgataaaaat atctttgatc ccaccgctgg tttctgagtt
    29041 ttttaccaat gaagtgatac aagccgaaaa gctaatgatc ggcaataaaa acaagaaagt
    29101 atttaaaata tgataccata ccgttatatc ccacgaggtg tggatcacta acagaaccat
    29161 tagaagtaag aagccactga acaacagcag cgagtaacta atcaaactaa aggttacttt
    29221 gcgattcaag tttcgtttgg taatgggcgc actctggttt cgtttactga tctctttgcg
    29281 attaaatgct agattgatga cgctgaaacc gctaaaaatg gatagaaaca acccataaga
    29341 caagaaatta aaggttgtgc ccgtcaacag ctttttcatc cgctgactat atcccgcact
    29401 tagctcaact tctccagtaa ccgagagtgc catttgtgtc tgttccatag ctgcatcgat
    29461 tgttttatct tcttcttgaa acagaatcag tgtttgcaaa aaggtgttca cttgctgtgt
    29521 aacaactgtt ttagaaaagg catctgggcg actctgtatc ttgatcgtcg gcatctttcc
    29581 tacttcgact tgttcggtaa agtctgcagg cagatgtaag ataatgttta cacgattaaa
    29641 ataaagagca tcatcgattt ctttttgtga ggttccatcc aatgtaacga ctgtttcctt
    29701 gtcattcaaa tatttacgaa ggccatcgac tacaggcgac tcttcttcac taatcaaggc
    29761 gattttcgca tcttgcagag aggtattttc aacatcaata ttttgcgcaa aaaagaatgt
    29821 ggcaacaaca gtgataatta cacctatcag taaattcgat ttatttgctt taagcaatgt
    29881 ccaaatcgtt ttataaagcg tcataacgca cccctctttc catccaagca tttcctaacg
    29941 caaaaaggaa aataaatccg atcaaccata gaagattggt ataaaaacta gccaaatcag
    30001 tgtagaaata aagtttatac aaactttcgc tgatcaagtt gacgagattt aattgaccaa
    30061 caatcggaat atgaagatca atccaatact tcacttgttc cgaccccatc ataccagcaa
    30121 aaaagctcat cgcaatcgtc actgaaatca agatgctatc cttttgcacc agattcattt
    30181 ttggtatcaa attaccaacc aatgtaccta acaaaatcgc acacaacgac cccagagcgg
    30241 taacaatcat caaccattgc caacgttcac caaagtctac ttgatagata aaacgaaaga
    30301 cagctaacat aatataaatc tctgtcaaaa caatcgtaaa gcttgctgcc aaattagcga
    30361 ttgaaactaa cgattgcttt ctaggaatca aggacaagcg aataccattt gacgattgat
    30421 ttgcttgctg atcctgtaca ttgcggaccc cccacatcgt cccgtagaga atcgccattc
    30481 caatcagagt gaagaaataa aaacttttga aactcatatt acgacctgca tgaacttctt
    30541 gaaaagatac ttcttgttca aaaacagtca tgatgggcgc gagatcaacg ttctccactt
    30601 ctgccacttt actcgaaatt ataccaatac gctgtaaaaa ttgatccatg acgtttttca
    30661 tcaacgtctg actcattcct gcttgaccca catggagcga aacagatgaa ccatcaatga
    30721 gataaaaacc agccaaatca tctgcattta gcttttcatt cgcttctact tccgatagtt
    30781 cctgtacttc aaataatggt ttatcattga cttcgacttg ttctaagact gtggtaaaat
    30841 tttccgaaag cgctgtttga tccgctttga ttacaattcc agtattaatc gtttcgagat
    30901 tgccaccttg atcaatctcg ccaaaggccg ccataaacat caatcctaaa agaattggaa
    30961 aggccaatgt ccaaaacaac atggatttat ttcgcagcag tatttttgtg cgatatttat
    31021 aaaaatgcca aaacattatt tcccctcctc tagtctctca aatccttgcc agtaatctct
    31081 aaaaagacat cattcaagga cgcttctttg gttgctattt ttccgtaact gatcttttct
    31141 tcttgtaaga ttgtaagaat cggcaacaat actgtattta gttcagtagt tgttacttgg
    31201 agctgacctt ttttataagc gattccatca gaagctattg ttcgctgaca acacgttttc
    31261 tgggagtaat gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat cttttagaga
    31321 ttctttggtt cctttagcaa tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag
    31381 aatttctact tcttccatgt aatgtgttgt atagacaatg gttgctcctt gtcgattcag
    31441 ctccttgata ctttctaaaa ttttattccg actttggggg tctacagcta ctgtcggctc
    31501 atctaaaaaa attaaccgcg gcttgtgggc aatcccacat gcgatgttca accgacgttt
    31561 caagccacca ctaagttctc ctggacgaaa cgaaacatat ttttctaatc ccactaattg
    31621 aatcacctct tcaatcaagg ctttcctttt ctctttttcg gaaacatata aaccgcaaaa
    31681 ataatcgata ttttcttgca cacttagttc gtcaaagacc gcaacgtctt gaggaacgac
    31741 cccaatcatt gccttgacag cataattttc aggagtcatt gttgtcccga acaatttaat
    31801 ggttcctttg tcataattta acaaagaaag catacagttg atggcagtcg atttcccact
    31861 gccgtttggt cctaacagac ctaagacttc tccttcttgt acttgcagat caaaatgatt
    31921 caatgctaca aaatctccat agcgtttgac taatttatca atttcgataa tcatcatgaa
    31981 aaactccttc caaatttctt gttgatctga ctataataaa gtgcaagaaa agaaactagt
    32041 gacgattggc taaaccacac atgacatttg tcacaatgaa ggagtctgct atgcataaat
    32101 ctcggctact caacgaaact agctttctac tggttatctc attgcttttg ctggtaagtc
    32161 aaggattcgt tgctatgcaa gttatctatt tattgatcgc cgttttattc actggtgtca
    32221 gttatttact gcctacaaag ccaaattgga ttttccaagt tattctattt cttttatgtg
    32281 cctttttacc tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag
    32341 aaagccacct tgattacttg tttttgttag gttttttgtt gatttcgacg gttggtaatc
    32401 atccccccat caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa
    32461 aaaaagatca acgaacgacc gatcttgaga aaaagtacat tgtctccaaa gatactcatt
    32521 gggaaaaaca acaagagtta tcggaacaaa ataaagcatt ggccctgtca caagaaacac
    32581 aattagcctt gcaaattgtg gaagaacgga accgaattgc ccgagacatc catgacaatg
    32641 tcggtcatct gctgtcaagt gcgattttac aaattggtgc gcttgaaatg atcaatcaag
    32701 aaccaaaact aaaagaacca ttagaaaatt taagtgccac cgttcacact gggatggatc
    32761 ggattcgtca aagtgtccat gacctccatg agacatcgat ctcttttcaa cactccttag
    32821 agtttctgat cgctgatttt ccttcttctg ttacgattga aggaaacttg tttgaaggat
    32881 tgaatgaaac gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta accaacagta
    32941 tcaaacatag tcatgccaaa accgttgttt tacattttcg aacgttgccg gccttttatc
    33001 gcttgcaaat cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt
    33061 tgaaaacgat gcgtcaacgt gttcaagaac aaaagggaca agtccatatt ttccaagaaa
    33121 accagcaatt tcagttaacg atcattttac caagggaggg acaccaatga taacagtagt
    33181 cattattgag atgatccatt tgtgacgaaa tcgttacaaa ccattttaga aagcacgaaa
    33241 gagatccgtg tgttaggcat tggtcattgt gccaaggatg cgctagtctt atacgaaacc
    33301 catcgtcccg atgttttgct gacggacatc cgcatgccta aacaaacggg catcgatgct
    33361 gccaaagaaa ttttggctaa atttcccaaa gccatccttt tgcttttgac gacattcaaa
    33421 gatgaagaat acatccgcga agcattttcc atcggtgtca aagggtattt aatcaaacag
    33481 aacttgcaag cgattattcc ttctgtcaaa tccgcttata atggacaggc cgtatttggt
    33541 aatgaaatca tcgagacttt cactcaaatg atgaagaata aaccgacgat tgatagtcat
    33601 gcttatgctt cttttagtga acgtgagctt gcaatcatca aagaagtagc tgccgggaaa
    33661 aacaataaag aaattgcaga tgccttgtac ttgagtgacg gcactgttcg caattacatc
    33721 agccaattac tagaaaagct agatttacgg gatcgcacgc aattggctat ctatttttat
    33781 cagcatttgc aataagtctt atctaacaca aaaaaacctg aatgatgctc cttcgcatca
    33841 ttcaggtttt ttt
//
LOCUS       DAJSVD010000023        31653 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSVD010000023 DAJSVD010000000
VERSION     DAJSVD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.023"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            277..1887
                     /locus_tag="ON498_000926"
     CDS             277..1887
                     /locus_tag="ON498_000926"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR3939219.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2179..3048
                     /locus_tag="ON498_000927"
     CDS             2179..3048
                     /locus_tag="ON498_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR3939220.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3213..4484
                     /locus_tag="ON498_000928"
     CDS             3213..4484
                     /locus_tag="ON498_000928"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3939221.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4481..5776
                     /gene="rho"
                     /locus_tag="ON498_000929"
     CDS             4481..5776
                     /gene="rho"
                     /locus_tag="ON498_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3939222.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5875..6138
                     /locus_tag="ON498_000930"
     CDS             5875..6138
                     /locus_tag="ON498_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR3939223.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6583..7032
                     /locus_tag="ON498_000931"
     CDS             6583..7032
                     /locus_tag="ON498_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3939224.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7089..8507
                     /locus_tag="ON498_000932"
     CDS             7089..8507
                     /locus_tag="ON498_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3939225.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8507..9367
                     /locus_tag="ON498_000933"
     CDS             8507..9367
                     /locus_tag="ON498_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR3939226.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9541..10314
                     /locus_tag="ON498_000934"
     CDS             9541..10314
                     /locus_tag="ON498_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3939227.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10461..10826
                     /locus_tag="ON498_000935"
     CDS             10461..10826
                     /locus_tag="ON498_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR3939228.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(10974..12182)
                     /locus_tag="ON498_000936"
     CDS             complement(10974..12182)
                     /locus_tag="ON498_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939229.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12201..12479)
                     /locus_tag="ON498_000937"
     CDS             complement(12201..12479)
                     /locus_tag="ON498_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939230.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12633..13328
                     /locus_tag="ON498_000938"
     CDS             12633..13328
                     /locus_tag="ON498_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3939231.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(13770..14567)
                     /locus_tag="ON498_000939"
     CDS             complement(13770..14567)
                     /locus_tag="ON498_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR3939232.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14564..15748)
                     /locus_tag="ON498_000940"
     CDS             complement(14564..15748)
                     /locus_tag="ON498_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3939233.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15773..17020)
                     /locus_tag="ON498_000941"
     CDS             complement(15773..17020)
                     /locus_tag="ON498_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3939234.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17235..18980
                     /gene="dnaX"
                     /locus_tag="ON498_000942"
     CDS             17235..18980
                     /gene="dnaX"
                     /locus_tag="ON498_000942"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3939235.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19000..19314
                     /locus_tag="ON498_000943"
     CDS             19000..19314
                     /locus_tag="ON498_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3939236.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19339..19935
                     /gene="recR"
                     /locus_tag="ON498_000944"
     CDS             19339..19935
                     /gene="recR"
                     /locus_tag="ON498_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3939237.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            19999..20643
                     /locus_tag="ON498_000945"
     CDS             19999..20643
                     /locus_tag="ON498_000945"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3939238.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20658..20987
                     /locus_tag="ON498_000946"
     CDS             20658..20987
                     /locus_tag="ON498_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR3939239.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21001..21939
                     /gene="holB"
                     /locus_tag="ON498_000947"
     CDS             21001..21939
                     /gene="holB"
                     /locus_tag="ON498_000947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3939240.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            21975..22799
                     /locus_tag="ON498_000948"
     CDS             21975..22799
                     /locus_tag="ON498_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR3939241.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22792..23139
                     /locus_tag="ON498_000949"
     CDS             22792..23139
                     /locus_tag="ON498_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR3939242.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23208..24080
                     /gene="rsmI"
                     /locus_tag="ON498_000950"
     CDS             23208..24080
                     /gene="rsmI"
                     /locus_tag="ON498_000950"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3939243.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24189..25310
                     /gene="dinB"
                     /locus_tag="ON498_000951"
     CDS             24189..25310
                     /gene="dinB"
                     /locus_tag="ON498_000951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3939244.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25364..25966)
                     /gene="nrdG"
                     /locus_tag="ON498_000952"
     CDS             complement(25364..25966)
                     /gene="nrdG"
                     /locus_tag="ON498_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR3939245.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26281..28470)
                     /gene="nrdD"
                     /locus_tag="ON498_000953"
     CDS             complement(26281..28470)
                     /gene="nrdD"
                     /locus_tag="ON498_000953"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3939246.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28769..29563
                     /locus_tag="ON498_000954"
     CDS             28769..29563
                     /locus_tag="ON498_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939247.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29553..31595
                     /locus_tag="ON498_000955"
     CDS             29553..31595
                     /locus_tag="ON498_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939248.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        10177 a         5659 c         6636 g         9181 t
ORIGIN      
        1 aagcttatta tgtgggttaa attttttttg cactcataaa ggacttaaga aataatttct
       61 caaaggatat cgaaaaagaa aaaattgtaa gcggatagta ttcatttgag tacttgactt
      121 tcccttagtc agcttgtatt atacaccttg ggcacccttt tagagggtca gaagtttaca
      181 gaaatttcgg ctccctattc ttattgaaga atagggagct ttgtttattg tctgagactt
      241 ttcctgcaca taaattaaaa aggagttttt gaacttatga caaaatatat ttttgtgacc
      301 ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag cttctttagg acgcttattg
      361 aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc catacatcaa cgtggatccg
      421 gggacgatga gcccatatca gcacggagaa gtattcgtaa cagatgatgg tgctgaaaca
      481 gatttggatc tgggccacta cgaacgtttt attgacatca atttgaacaa atactccaac
      541 gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag aacgtaaggg cgaatacttg
      601 ggagcaactg ttcaagttat tcctcatatt acaaatgaga tcaaagaaaa aatcatgcga
      661 gcagctaaaa tgactgattc agatgtgatc attacagaag tagggggaac agtaggggat
      721 atagaatctt tgccattcct tgaagcactt cgtcaaatga aagcagatgt cggtacagat
      781 aatgttatgt atatccatac aacattgatt ccttacttga aagctgctgg agaaatgaaa
      841 actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt taggtatcca accgaatatt
      901 ttggtggtac gtacagagca acctgtttct caaagcgtga aaaacaaatt ggcacaattt
      961 tgtgatgtcg aaccagaagc agttatcgag tcgccagatg tagatacact ttactccatc
     1021 ccattagttt tacaagcaca aggaatggat caaattgttt gcgaccattt aaaactagat
     1081 acaccagaag cagacatgac agaatggaaa gaattagaag aacgtgtctt gaacttgaag
     1141 aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt taccagatgc atatatctca
     1201 gtagttgaag cattgaaaca ttctggtttc gcctataatt cagacatcga gatcgattgg
     1261 atcaaagcac aagaattaac gagagaaaat gtagaagaac gtttgaaaga cgcagatgga
     1321 atcttggttc ctggtggatt tggtgatcgc ggagtagaag ggaaaatcga agcgattcgt
     1381 tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc ttggtatgca gatggcttgt
     1441 gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt catcagctga aacgaatcca
     1501 gatacagcaa acaatattat tgatttaatg gctgatcagg aaaacatcga gaatttgggt
     1561 ggaactttac gccttggatt atacccatgt aaaatcaaaa aaggaacgaa gacggctgaa
     1621 gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc accgctatga gttcaataac
     1681 aaatatcgtc aattgtttga agaacaaggg ctagtctttt caggtgtctc accagataac
     1741 cgtttagtag aaatcgtaga gctgccagag aaaaaattct ttgtgggatg ccaattccac
     1801 ccagaattga tttctcgtcc aaatcgcccg caaaaattaa tcaaagcatt tgtcggagca
     1861 tcattagcag atcacgaaac aaaataattt ttgaacttga tagaaagaga aaagtagcaa
     1921 gagaagggga taagcaattt tcttgtcact tttctctttt tctgatttgt tattatttgt
     1981 aggaaaaaag atagaaagca aaagactttt ttcgattaaa gcgctattgg atattttatt
     2041 ttttgcaaaa aacttcttga aaattttcat tttcattgat tttagtagaa aactaccgaa
     2101 atgtttggta aaatagggtc gttggttaag aattgtctta acaaatttag ttttacaaaa
     2161 cttaggagga attttattat gccagtagta tcaggagctg aatttttaaa agctgctcgt
     2221 aaaggcggtt acgctgtcgg cggattcaac acaaacaact tggaatggac tcaagcaatc
     2281 cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc aaacatcaat gggtgcagct
     2341 aaatacatgg gtggttacaa aatctgtaaa gatttaatca ctgatttagt tgaagcaatg
     2401 aacatcactg ttccagttgc tatccactta gaccatggtg attacgaagc tgctttggaa
     2461 tgtatcgaat taggctatac ttcagtaatg ttcgatggtt cacatctacc atttgaagaa
     2521 aacttgaaat tagctaaaga cgttgttgaa aaagcacatg caaaaggtat ctcagttgaa
     2581 tgtgaagttg gttcaatcgg cggagaagaa gacggaatca tcggtaacgg cgaattagct
     2641 gatattgaag aatgcaaaca aatggttgct acaggtatcg actacttagc ttgcggtatc
     2701 ggtaacatcc atggtcaata tccagaaaac tggaaaggat tagcttttga ccacttacaa
     2761 gcaattgctg atgctgtagg tccagacatg cctttagtat tacatggcgg atcaggtatt
     2821 cctcaagatc aaatcgaaaa agctatctca atgggtatct caaaagtaaa tgttaacact
     2881 gaattccaat tatcatttgc tgcagctact cgtaaatata tcgaagctgg taaagataaa
     2941 gaaggtaaag gattcgaccc tcgtaaatta ttagctccag gtaaagcagc aatcgttgaa
     3001 gatgctaaat ctcatatcga ctggttcggt tcagctaaca aagcgtaagc atccacataa
     3061 cagatacgat atataggaat ggagaacgac ttttgaagtc gctctccatt cttttttact
     3121 taaaacggca gaccatgctc gtttcttttt tttatctatg ataaaataca agcatatcca
     3181 tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc aaaggaaacc
     3241 gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc gcattgattc
     3301 cagctgcaat cttagctgat tccccagtga tcttggaagg tgtgccggac atacaagatg
     3361 ttcattcatt aattgaaatt ttagagatca tgggcgcaac tgtccatttt tccaacaaca
     3421 tactagaaat cgatcctcga gaagtcgtat cgattccgat gccgcacgga aaaatcaata
     3481 gtttgcgtgc ttcttattat tttatgggaa cactgcttgg taaatttggc gaagcggtag
     3541 taggcctgcc aggtggctgt tatttaggcc ctagaccgat tgacctacac gtcaaaggat
     3601 ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc catgtattta cggacagaaa
     3661 ataaaggatt gcgtggcaat cggattttca tggacgtggt ttcagtcgga gcaacgatca
     3721 atgtgatgtt agctgctgta aaagcaaaag gacagacagt aattgaaaac gctgcacgag
     3781 aaccggaaat catagatgtg gcaactttac tgaataatat gggggcaaaa gttcgaggag
     3841 cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt acatggctgt cggcatttca
     3901 tgattcctga ccgcatcgaa gcaggaacct atctagcatt agcagctgca gtaggaaatg
     3961 gtatcaaagt gaaaaatgtg atctttgaac atttggaaag ctttatagca aaattgcagg
     4021 aaattggtgt ccatatgaaa atcagcgaag atgaaatcga agtttacccc tcgaaagaat
     4081 taaaacccgc aaacatcatg acctatcctt atccaggatt tgccacagat ctgcagcagc
     4141 cacttactgc gttactattg atgactacag gcacttcaga aatcatcgat acgatctatg
     4201 cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg cgctgatatt accgtggaag
     4261 gaaatatgat catcgtgaat ggtccaaata aacttcacgg cacagaagtc gtggcttctg
     4321 atcttcgggc aggtgcttgt ttagtgattg ctggactatt ggcagaaggg acaacgacga
     4381 tttacaatgt agattatatc ttacgcggtt atgatcatat tatcgagaaa ctaacagcct
     4441 taggggcatc aattgaaatg atagaggaag tgggagctga atgagcgatt atttgacaat
     4501 ggcagaacta gagaacaaaa cgttaaaaga agtttatagt tatgccaaag aatttaaaat
     4561 tccttattac agtaagatga ataaaaagga attatctcta gctgtgattc gtgcacaagc
     4621 agagaaacaa ggattttatt atatggaagg aatattggat atcgttggtc aagatggtta
     4681 cggtttttta cggccaatca attatggacc aagtgcagaa gatatctata tttctgcctc
     4741 acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag cccgtccacc
     4801 taaagaatcg gaacgttatt atggtttgat gcacgtggaa agcgttaatg gaaaagatcc
     4861 ggaagaagca aaagaacgtc cgcattttcc agccttaaca cctctttatc cgcaaagaca
     4921 gcttcaactg gaaacaacac cgcaaagact atctacccga atgatcgata tcttttctcc
     4981 aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga aaacttctgt
     5041 attaaaagaa atcgcaaatg gtatcaccga aaactatccg gaagtagaat taatcgttct
     5101 actgattgat gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga aaggcgacgt
     5161 ggtatcttct acatttgatc tgcagccgca aaaccatact cgagtttctg aattagtatt
     5221 ggaacgcgca atgcgtttag tcgaagataa acgagatgtc gttatcttga tggatagtat
     5281 tactagactt gctcgggcct ataacttagt cgttccacca agcggacgta cattgagtgg
     5341 cgggattgac ccagccgcat tgtataaacc taagaaattt ttcggggcag caagaaacat
     5401 cgaagaaggt ggcagtttga cgattttagc gaccgcttta gtcgatacag gaagccgtat
     5461 ggatgacgtg atctatgaag aattcaaagg aactggaaat ctagaattac aactttcacg
     5521 tgaattatct gaacgtcgga tcttcccagc catcgatatc aaaaaatcag gtacccgaaa
     5581 agaagaatta ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc gaaaaaatat
     5641 gactggagat tcattagaat atacggaaca actgatccgc tttttgcgta aaacaaaaaa
     5701 caatcagcaa tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa atccgaccag
     5761 aaatctgaaa agataattgc aaccttgact gaaacatgtt aagatgttaa tgttgtaaat
     5821 cgacaaatca actctggttc agcaaatgga gcagggcaaa ggagcgaaaa aatcatgaaa
     5881 gaaaacatcc atccagatta ccacccagta gtattcatgg attcaacaac tggtttcaaa
     5941 ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg aatgggaaga cggtaataca
     6001 tacccagtca tccgtgtcga agtaacttct gactcacatc cattctacac tggacgtcaa
     6061 aaattcacac aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg tctcaaagac
     6121 gcaaacgctg cggaataata aaaacgctga taactcaacg tttagcagac tatctgatag
     6181 ggatagtctg tttttttatg ttttttgcca atttaaaact tcgtttgatg ctgaaaaagt
     6241 cgtaggatca taatgcacaa aacaatgaaa aactagtata tagttttaat tatccacttg
     6301 ataaaaacac cttatattaa cgaaatgaag aggggttatt tcgaagtaaa aaaccattta
     6361 cctaaaatta gaagctaagc tttcctttca aggaaggctt tttgttttgt tatagacttg
     6421 cgattgtttg tgagctagat cgttcatttt aatgacttat acacgcggat gatgggtctt
     6481 tttcctctgt tcattcataa tattgaacgg tacagctagc gatgctatac tgacctcagt
     6541 tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt tgatcaattg
     6601 atcgcagaag aactgattgt tttcgatcca tcgatagatt caaaaggatc attatttgag
     6661 cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa agtcgttaag
     6721 gcactattca aacgagagga ggagatctcc acaggaatag aagatggatt tgggattccc
     6781 catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt tttttcatac agccaaatta
     6841 acggactata gcgggataga tgaaatgcca attgaatata tttttgccat cgtagttcct
     6901 gaaaaatcag cagatatgca tctagagatt ctcagcacac tctccagacg attgatgaat
     6961 gcagatttta gaaagggaat aaaagaagcc actacgccag cagatatcat cagacagatt
     7021 agtcaagaat gaaaatagta gagtctttgt ccgagtatcg atagtgaaga aaaaggagga
     7081 aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct catacttata
     7141 tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca aaattcgaaa
     7201 cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga aatcaaagag gcagatgcaa
     7261 tcattcttgc agtagataaa gaaatcgaat tggaccgatt tgccgggaaa aaagtaaagc
     7321 gtgtctctac aagccgagca atcaaagaac cgcaagtagt aatagacgaa gcattgagag
     7381 atattggcgt attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag aaaaagccga
     7441 caatctataa tcatttcatg aatggtgtga attacatgct tccatttgtg atcgcaggcg
     7501 gaatcatcat cgcactgagt tttgcctttg ggattacagc ggctgatccc aactcagcag
     7561 actataatgt gttagcagct gcattttcaa ggatcggagg agatacagcg tttgccatga
     7621 tggttcctgc gttaggcgcg gggattgcta catcgattgc tggaaaagca ggttttgccc
     7681 caggaattgt tgccggatta ctggcctcta caggtggttc aggttttctt ggcggaatga
     7741 ttggtggcgt gctagcaggg tatatctgtg attttctggc aaataaaatt agcgtaaaaa
     7801 aagaattttc tgctatttat cagctgattg tcgtcccctt tttatcgatc ttaattattg
     7861 ggcttttgat ggtatttgtg attgaacaac cgatcacatg ggtgttggat gctttgacaa
     7921 attggctgaa tagcttggga aatacatccg ggctattgtt cggattactt atcgggatca
     7981 tgatggcagc tgatatgggt gggccgatca ataaatcgac ttcgactttc tcgatcggtt
     8041 tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat ggctgcagga atgacacctc
     8101 ccctaggtat tgctttagcc actttattat tcaaacataa atttaccaaa gaagaacgga
     8161 cttcaggtca atcatgctgg attttaggcg cttcgtatat tacagaaggt gctataccgt
     8221 ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat gacaggttca gcagtcgctg
     8281 cagggatttc catggcagta ggagttactt caatggcacc tcatggaggg atttgggtga
     8341 tgctaattcc aaatgttatg aacaatttac ctatgtatgt cttggcaatt gcatcaggta
     8401 cagtcactac agcctttatg cttggactat tgaaaaaaac agtgaaagag aaaaaacaag
     8461 acaagtggac gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt tatatacaat
     8521 gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct tcaatatttg
     8581 cagctatgat atgatgaaag caatcatcga agaagcagag agattagaag caccagtaat
     8641 cttagaaatt catcctgatg aaatcgccta tttaagtgat gagtttgttg cggcagtaag
     8701 agcctatgct tatacaagta aactgccgat cgttatccac ttggatcatg gcggtacgat
     8761 agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg atgcgtcttt
     8821 gaaatcattc gacgacaatg tacagattac gaaaaaagtg gttgcgttag ctcatgcggt
     8881 aaatgtctca gtggaggctg agcttggaac gattgggaat aatggttctg ctgaaggagg
     8941 tgctgaaaca atcatttata cagatcctga tcaagcgaag atttttgtga aagaaacagg
     9001 aatcgataca cttgctgtag caattggaac agcgcatggc ttgtatccga aaaacagaac
     9061 tccaaaatta aatatcgact tactgaaaga attgaaccaa caaatcgatc ttccatttgt
     9121 tttacatggc ggttcaggta atccggacgc agaagtcagc gaagcagtaa aatacggagt
     9181 tgccaaagtg aatttgagct cagatcttaa aagcgtcttt ttcaatgctt tgagagaaat
     9241 attgagtgaa aatccatcga tgtacgagcc gaatgcaatt tatccagcag ctaataaaaa
     9301 ggtacaagaa gtggtcaaac ataaaatgga aattttgaac acagtaggca aggctagttt
     9361 atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa tagtcttgtc ataccctctt
     9421 aaccacacac gatgttcaga agtagttttt tcgaaaaatt tcttcttatt tctcgaagcc
     9481 aatcacttct gtcacaacct cttttttcct gttactatat aaagtaaatg gggtgatttt
     9541 atgttgaatg aagtggagaa tagacagcag gagattgtga attatctaaa aaacaaccaa
     9601 tttgcaagag tcaccgattt gatcgagtta gtaaattata gcgaagccac gattaaacgt
     9661 gaccttgttt ttctggaaaa aagtggattg atccgtagag ttcgcggtgg cgccatgctt
     9721 attgacaatc gaaaaattga cgtgccttac ctgatgaaga tcaccaaatt agaagaagaa
     9781 acagaaaaac gtaagattgc agatacagct tcttctttga ttcgagatga tatggtctta
     9841 ttcttggatt ccagtacaac ctctttacac ttagtcagaa acctagcaag atttgatggt
     9901 ttgcagatca ttacgaatgg catcgtcaca gctgctatgt tgagtgaata tacaacggca
     9961 aaagtatctg tattgggagg ggcaattgtt ccgaaacgag caacaatcaa cggttcaaaa
    10021 gcctataacg atgcattgtc atattatgcg gatttagcga taatgagttg tcgcgggcta
    10081 gatttcagcc aaggagtaac agaaactcat gaaggagagg caatggtcaa aaaagctttt
    10141 agaaaacaat cagaccggct tgccgtttta gttacacagc aaaaaattgc acataaattc
    10201 atgcatcaag ccattgcttt tcatgaaatc gattatctgg tgacaagtaa agaactgaca
    10261 aaagaacagt atgcgaaagt aaaggaacac catatcgaat gtctgtacgg ataaattatt
    10321 acgtgaatgt tctaagtggc ttcctcattt ttagatagga ccttatcttt gctcgcttct
    10381 ctcagccgat ggtataataa tgaaaaggtt atcttgttag ataatcttac ttttttatcc
    10441 aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt ggtagtgaag gcaatcatga
    10501 tctgggcgat tctgtgggtc gtgctgacag gtttatacgg tgttagcttt atggattcaa
    10561 cgattgttgg ggtaattatt gtagtcatga tctatgtgct aggggatctg ttgatcttac
    10621 gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg ttcagctgca gttatcttgt
    10681 ggctctatct atattttatg aatgatacag tcgatatttg gatgaaagta ttgattcctg
    10741 ctctattgat cggtgtggca gaatggttct tccacaagtg gttgctaagt caaggcatcg
    10801 taccagatga acggaaaatg gaataaatca acagttaaac tattcttatc gataaatgaa
    10861 agctttaaaa ataaatagat gtttaattaa gaataatcat aaactatttt tactcaaaaa
    10921 accgctcaga ttattttcag atctgaacgg ttttttttat ctattcatca agatcaaatt
    10981 tcatttttca atgattcaat caattcgccc tctgcttcat gactatgttt tcggccatta
    11041 acaagataga gtgctgtaca tatcaatgga atgactccgg caatcacata caatcctcta
    11101 aatgaagtaa gagattttag catccccatc aaataaggac caacgcctaa gcctaggtct
    11161 aaaccaataa agtaggtaga taaagcaata ctgattcgat gttcgcggac gatttttaaa
    11221 caaacagcct gtccatttga cataaacgta ccataaccta ggccgactaa tgctccagag
    11281 ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa ataatcctgc tgttaagaat
    11341 aaatagctag gatacatcac gtattgttct cctttgacat cgaaaatcct tccagtcaat
    11401 ggacgtgtaa atgtgatgac caaagcatat acgacgaaga aaaatgaccc agcagcaact
    11461 aagtggatta cttttgtata agaagataaa aatgataaaa cacttgaata agaaagaccc
    11521 atcaaaaagg caattcctgt aatgaaaagt gctttctttt caataaaact attgatattc
    11581 catgagcgaa gagatatttt ttgttcttca gataatggaa tattttttac aggaaagaca
    11641 aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa accagataat gaaacgaaaa
    11701 tcagtcatat tcagcaaaat cattcctaaa aatggcccaa cagcggcagc taaactagta
    11761 cttaatccat aataattgat gccttcccct tttttagatg cagggatata tgcagtaacg
    11821 atagcatttg tagctgtaga aactgtaccg tagccgaatc cattcaagaa tcgaactaaa
    11881 tacaatgccc cgatagttgg cgtataaaga tatgctaatg ttgaaaggag atagaagatc
    11941 gcgccgcctc ttaaggtaaa cttccgacca aatagttcta actgtttccc catgaataag
    12001 cgtgccagca atgttccgat gatatagatc ccagaagcaa aacctgcttg gctcaagctg
    12061 gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca tcaacagata ataaatcagg
    12121 tagacaataa aattgatcaa tgtgattcca ataaaatatt tattaaataa tttcttttcc
    12181 atgtatgtta tacattcccc ttaagctgaa tatgctttta atttttgtat ttgaacttct
    12241 acatcgtgat aattggatgg atagcgatca acaagttggc gaataaataa atctcttgta
    12301 gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg gattctcatc cactgctttg
    12361 atcaattcga tcgagttttg atacgcatca tttaaaaaga ctaaagaagc tttcatatca
    12421 aatcttgttt gatatgctgt ttctaatcga ttaatgattg tgttcatcca gtaattcatg
    12481 ttttttccct ctattcttaa aaaattttac ttctgcagta taacgaataa aaaaaaggaa
    12541 aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg ttaatatcat tagtaaaata
    12601 cctttggact ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa agtttggatg
    12661 ggggagcatc attggatagt agagtcttaa aggaatattt ggataaaaat gattttccga
    12721 ttatacgcaa aggatatcat aaatatttga cattcgaagg gatggaagat tcccatacgt
    12781 atatcttgaa agagggaacg gtaaaagcaa gtgttatttc aaatgatggg agagaattta
    12841 accttcgcta tatcaaagat cttgaaatcg tctctctttt aaaagacgaa tattcacaat
    12901 tcatcgattc gccgtataat attcgagttg aatcagaata tgcagagttt tatcgaatca
    12961 atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa gctacaacac tatgtaaaag
    13021 agtattaccg ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg ctattaaacg
    13081 gaaagctggg agctgtttgt acgcaacttt acgaattata tgatttattc ggtgtgcgga
    13141 cagaagaagg gatgctgatc gatttttcga tcacgaatga agaaatcgcc aaattttgtg
    13201 gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa gatggtatta
    13261 tcaccgttaa agaccatcat atcgttattt tgaatatgga cttgttagaa gacaatatta
    13321 tcttttgatg aacaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    13381 acggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    13441 tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca caaggaacaa
    13501 tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga ggagttggta
    13561 ggagaacacc gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt aataagaaga
    13621 gccaagtaaa aacagtctct tatgttttta acttggctgg acttatcacc gtagatgtaa
    13681 gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga cacgattttt gtcacagccc
    13741 tttttttgcg aacattcatt tagcaatttt tagaaacttc tgccgccacc gccaaaagtc
    13801 ccgccaccgc tagaatgggt agtacttccg ccaccgccgc ttccaggagg tgggcttttg
    13861 gggattcttc ttgtcgtgac aaaagaattt gtcagacgat cggttttttc agttaatttg
    13921 atactggatt tttctcggaa tggatatttg tagatgcctg atttcaattg ataacgcgaa
    13981 acagtcacaa aataaaagac aagactaagg ataagagcca aaacaacagc aatgatgatt
    14041 tctactgtcg tcaaaacctt atagcgtgta attttgccag tttcttcgtc tacacgataa
    14101 tgtcctcctg gaactccgtc ttccacatat gttgctgatt tagacaggta agcttttgca
    14161 gcattgtaat aggcagcatt tgtcatctga tcataaacat catctaagat cgaatcgatt
    14221 ctgctatcag tcaaataatc gatcatatta ccagaagtcg aaatgtagat ttcacgctgt
    14281 cccatatcta atagcaatac tgagccattt tgatcatttc cgactgcgtc tcttaagtaa
    14341 ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag tcgttacgat caagactcgt
    14401 cctttgattt tttcattcag cttttctgcc tgttgttcca aagattggat ttgatctgtt
    14461 gtgaataacc cagcctcatc atttaccgta gctgtatctg catgagtagc caccggccag
    14521 ccaacactta ctagcaaaag aaagaataaa aaaaattttt tcatcatatc aagtaacctc
    14581 cgatcagaaa gaggatcgct agcccagcga aaatcccaag agcggtcagt cccaattttt
    14641 tctggctgat cggcaggaca ccgctgacct ttcctgtttg tccattcatt gcgtagtagt
    14701 agactttttt acttgagtca ttgcttcgat aagtaacaag ccataccggc agtaatacgt
    14761 aattatttac ttcgtttgtg attgaagcag aagttcgcac accggtcaat gttgtatatc
    14821 catttgcggt atcttttaac agcttctctg aatagtcttt taattcagat tgaatctgtg
    14881 atttgattgc ctcatattcg atatctcttt tttctgcttg gaacccggct aaatactggc
    14941 ttttaaaatc aattgcttta tctaacggga aaggttgcac gccttcgacc atcttttgtt
    15001 gcgtattttt tgataaggca ttttttacta gttctttaaa agaaagcttg cctgctcttg
    15061 aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat atgcggataa
    15121 ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa taagggaaat
    15181 acacacctgt caacttatcg atttgatcct tattgaaaaa agctttggga acaaaccatt
    15241 ttttcttggt ccaagcgaga aatctgttga ttgcttcttc tttctcgaca gcaaaaggaa
    15301 gtactttatt tggcaagaat tctccgctta aacgtcccga tagtacgacg ggattatggc
    15361 agtagtaaca ataagtcgca gcagttgtcg catcagtcac gatctctgca ccacagttgg
    15421 gacaaagaaa aagatctatc gctgcggtat tatctgcaga ttcgtccgct gtatctgaaa
    15481 aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt catatcggca agctgttctt
    15541 gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc tttgctttct tcgccagcca
    15601 tacgcgcttc tttttgcttt tgctcatatt ggctgacttc ttcttctgta tagatattca
    15661 ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga ccgccgcaat
    15721 taggacattt atgtgtaaga acatccatca gctgcacacc tttctaaata aattaattaa
    15781 cggggattcc tttaaacggt ttaccacagt ttggacagaa tttagggatg ccgttggaaa
    15841 gatccacgat ctcattgcat tcactgcatc gcatttgtag cttaggctga acgttttcta
    15901 caggtttggc agctccgcag ttggaacaga attttccgga attttctgtc ccgcaaactg
    15961 gacatgtcca agtatccgca tttccttgtg cagtttgttg attttgtttt tctgcttgtt
    16021 gtttgatttg ttcttggtta ttttgagctg cttgagaaag ataagaacca tttgcattca
    16081 ttcccatacc tacacccata aagccggtca ttgctcctgc ttcatttttt ccggctgctt
    16141 ccataccacg tgcaatggaa ccttgcacgt atccttcacg tacgctagga tcacctagca
    16201 tcgccccttc gttacgcata ttgatcagtt tcacagaatc atcggtgtaa gaaatgctgg
    16261 ctacagccac agaaacaatc tccatcccac gtaattcacg ccaagaatca tccaatgcag
    16321 tatccatgta tttgcttaat tccaaactct tagatggtac ataagaaatc cgttgaccat
    16381 ctgcagacat ctgattgatt gctgattgta gcgctgttag gaattcagct aaatactgct
    16441 cattgatatc attgatttcc acttgtgttt tgtttttagg gattgcgttt gtataaaata
    16501 aaatcgggtc aacgacatga atagaataag tgccatgtgc ccgtaagaat aattctgcgt
    16561 tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt ccctttattt
    16621 cttggagatt gatataaaaa acttgttgtt tctgcggtgt gacgccgcca aatttgaatc
    16681 ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt gaacatggaa ggagctgcgt
    16741 catttttaat ggtgtaataa ccttcctcag cggtataatc gatgattttt ccaccatcga
    16801 ccaaaagcat catcatgttt ggatagacat ggacgactgt tccgtctgtt aaaacatctt
    16861 ctgttccttt acgattggag ccacgtttgt catctttgcg gactttgaca ccttttgtca
    16921 tgaccgttgt atcactcata ttgtctggtt cgatgacttc tagccattga tctgccagac
    16981 cgccgccaac catacttgtt gctgctttga taagacccat aaaaaattcc tcctctgatt
    17041 cagctagtgc caaacaatta ttgtttattt attataccat aggaagatgt gaaacttctc
    17101 ggactagaga cggaattgaa atggaaaatt gcgtattgtt taaatacctg aaaaagccga
    17161 gaaggatagt agttttttta cctaatatca cgtataataa gagatagtga attttttaaa
    17221 aaagggggat ccttatggca tatcaagcac tttatcgagt ctggcgttcg cagcgtttcg
    17281 aagacatcgt tggacaaaaa gctgtgacac agacactaaa aaatgctatt gtttcccaca
    17341 aaacctcaca tgcctacttg tttacaggac ccagaggaac cggaaagacg agtgcagcaa
    17401 agattttcgc aaaagcaatc aactgtccga acagtaaaga cggtgagcca tgcaacgaat
    17461 gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa atcgatgctg
    17521 ccagcaataa cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat tatgcgccta
    17581 caaaggcagc gtacaaagtg tacattattg atgaggtaca tatgctttca acaggagctt
    17641 tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag tgtcatattc atcttagcaa
    17701 cgaccgaacc tcacaaaata ccagctacga tcatttcacg gactcagcga tttgatttta
    17761 aacggatcaa tacggcagat attgtcgaac atcttgaatt tatcttgaaa gaatcgacta
    17821 tcagctatga accagctgca ttgaatgtga ttgcccgttc tgcagaaggc gggatgcggg
    17881 atgcattgag tattcttgat caggcaatct cgtttagtga aggaacaatt actttagatg
    17941 atgccatgca agtgacaggt agcttaactg acgaaatgat ggacagttac ttgagtgctt
    18001 gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa ccaaattctc gcagccggca
    18061 aagaatcacg tcgctttcta gaggatatgt tacaatactg ccgtgacttg ctgatgtatc
    18121 agcaagcacc taacctagtt gaggaacaat cgagtacgat cagaagtaca tttacagaat
    18181 tggcacaaac tgtttcagcg gatcagctgt tttcaatgat aaagattttg agcgatactc
    18241 aaaatgaaat tcgttttacc aatagtgccg ctatttattt ggaagtcgct acagtcaaac
    18301 ttgcacagct accgtctgct tctgttcctt cagcagatgt agcatttgca gatgatcaga
    18361 tagtaaaaca gctacaacaa gagattaagg aactaagaaa cgaaatcaag gaactacgca
    18421 gtaatacagg aattgagaat acaaaagtac agccggtaca gcaaacaaat cataaaaaac
    18481 agacaagtac ctatcgtgta ccgacggaac gagtatataa agttctgcgg gaagcgacaa
    18541 gacagcatct gaaccaagta aaagaagtat gggaagactt gctgatgagt ctatctgtca
    18601 ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc tggacctaca ggactagtga
    18661 ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa ttgcagcttt
    18721 cggtacacaa caacttaagc cgaatgatca aagattatgc gcctgatcct gtttatatta
    18781 caagagaaag ctggccagaa cttcgaagaa attatttgac gcaggcacaa gaagagggaa
    18841 cagaaaactc ttttgcttct gaagactcag aaggagaaat cgagttgctt cctcaagaac
    18901 aagaagtacc agaagaaaca cttgtagtaa aagcagagga actgtttggc tccgaagctg
    18961 ttacagtggt agatgattaa taaaaaggag tagataatta tgatgcgtgg aatgggcaat
    19021 atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga agcacaagag
    19081 ctgctaaata aaacagagtt tacaggtaaa gccaccaatg aattggtcaa agcggttttt
    19141 acaggcgatc gtcgcatgaa agatatccaa atcaatgaag aagtcgttga tcctgatgat
    19201 atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc acaaattgat
    19261 gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt ttagttttaa
    19321 actgaaggag taatgaatat gcaatatcca gaaccaattg cgaaactgat cgacagctac
    19381 atgaaacttc caggaatcgg tcagaaaaca gcgacaagac tcgcctttta tacgatcgat
    19441 atgaaagatg aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa acgcgatttg
    19501 catttttgta gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat atgtagaaat
    19561 ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt aatggcaatg
    19621 gaaaaaatgc gagaatatca cggactttat catgttctcc atggtgtgtt atcaccgatg
    19681 gaagggacag gtccagaaga catcaatatt tcttctctgt tgcagcggct gcatgatgca
    19741 gatgtaaaag aagtgattat cgcaacaaat gcaacaacag agggagaagc aacagctatg
    19801 tatctctcac gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc acatggtctg
    19861 tctgtaggct ctgatatcga gtatgcagat gaagttactt tattgaaagc tgtcgaagga
    19921 agacgtgagt tataaatttc taattaagtg ttttttcaga tacaatataa aagcagcaca
    19981 agagatgggg gcaacaatgt gaacggacta tttattacaa tcgaggggcc tgatggtgca
    20041 ggaaaaacca gcatcttgaa tgaactttac cctcttttga aagaagtagc aaaaacagaa
    20101 atcattcaaa caagagaacc aggaggcatt ccgattgctg aagagatccg agcagtgatc
    20161 cttgatccaa aaaatgatcg gatggatgaa cgtacggaag cactgcttta tgcagcagca
    20221 agaagacaac atctagtcga aaaggttctg cctgctctgg caaaagggaa aatcgtttta
    20281 tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg caggccgacg catcggcgtt
    20341 accgagattg ctcggttgaa tgagtttgct acagaaggta ctacgccgga ttttaccctt
    20401 tatcttgacg tagattcaga caccggatta agaagaatca agaaaaacag acaaaatcaa
    20461 atcgaccgtt tagattcgga aggtttggaa ttccaccagc gtgtacgtca tgcttactta
    20521 aagctggcag aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa aagctttgaa
    20581 gaagtgctgc aaacaagtta tcatacaatt attgaacagt atccgcaatt ttttgaaaac
    20641 taggaaggtg aacgtttatg aaaattattt tagcgattat ccaagacaaa gacagcaacc
    20701 gtttatcaaa tgaattgatt gatgcaaata tccgtgccac aaaactatca tcgacaggcg
    20761 gcttccttaa ggctggaaac agtacgttta tcgtgggtat tgaagatgac cgtgtagaag
    20821 aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta tctacacctg
    20881 ttacactgga tatttcgatg gatgggggcg tcccttatcc agtagaagta gaagtaggcg
    20941 gtgccacagt cttcgtctta cctgttgaag gattccatca attttaggag gagctgagct
    21001 atgaatcaag aaataaactt ggctcaaatg caaccgatcg tgtatcagca gcttcaacgg
    21061 agttttgagc atgggcgtat cgctcatgct tatctttttg aaggggagaa aggaacagga
    21121 aaacatgaag taggtatatg gcttgcacag cacctgttct gtacccaaat gaaagacaat
    21181 ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca tccagatgtt
    21241 ttggttattg aaccggaagg acaaaccatc aaagtcgatc agatcagacg tttgcagacg
    21301 gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa agaagctgaa
    21361 aaaatgaata gcagtgcggc aaacagtttg ttgaaattct tggaagagcc ccctggagat
    21421 tttttagcgg tgttagaaac agatgcagta ggaagaattc tccctactat tcaatctcgt
    21481 tgtcaagttc tgcattttca agaactttcg aaagaagcac tgatccatcg attgcaagaa
    21541 gagcagattc cattggaaaa agcaaaactt ttggctttct taacaaacag tttaggaaaa
    21601 gcagttgaaa tctctcaaga tgaatggttt aatgatgcta aggacttgat ttatcaatgg
    21661 ttcgtttact tgcaaaagca agatactcaa gcctttatct atgtccaaaa aaaattggta
    21721 aaatcattta aagaaaaaac acaacagttt acaggattat ctgtcttgat gttttattat
    21781 caggaagcac agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa acagataaac
    21841 cagataattg aacggatatt gatcgcagag cagaaactac gcagcaatgt cagttttcag
    21901 gctgttgcgg aacagttcgt tttgcatacg attttttagc ttgccgcaaa ggatgatgat
    21961 caaggaggat ggaaatggta gaagtagtag gtgtccgata tagagatgca ggtcatatct
    22021 attattttgc accaggagac tctgaatatg tttatagtga caaggtatta gtcgaatctc
    22081 agcaatcctt acatattgct actgttgtcc ttcctaaaaa agagattgat cccaatgacc
    22141 ttcctgaagc tatccgtccg atcttgcata aagcgtcaca agctgaatta gaaaaagaca
    22201 aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa gtgcagttgc
    22261 gtgagttgga aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat aaattgattt
    22321 tttctttcac agcggacggg cgaattgact ttagagaatt ggtaaaagat ctggccgctg
    22381 tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca aagataatag
    22441 gcggaattgg tccatgtggg cgtccattat gctgttcttc ttttttaggc gatttcgtgc
    22501 ctgtatcaat caaaatggca aaagatcaaa gtttatcttt gaatccaaca aaaatttccg
    22561 gtttatgcgg acgcttgatg tgctgtctgc aatatgagag cgaagaatac gagacagcga
    22621 aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa gggaaagtcg
    22681 tagggttgaa tctgcttagc cgtatcgtta gtattcggct gactggacgg gaaaatcctg
    22741 tcgaatatga atgggatgaa ttgaaagaat ttatccaagt aggtgagatg aatggataag
    22801 agatcattat acgacggatt gaatcaatta gaatcagagt tgagaaatac attaacagaa
    22861 cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac tttggagatg
    22921 gaaaatcaac gtctgcggga acacctgcgg gaattgaacc aagttgcaca agctccagct
    22981 gaaaatgtga agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat ttatgaagaa
    23041 ggattccatg tctgctacga tttatatgga tcaagaaggg acaatgacga gccttgtgcg
    23101 ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac caataacaag aggagactgg
    23161 agcggagaaa tactgctcca gtctttttat taaggagatt gtgacagatg tataatcaaa
    23221 aaagtttcaa gggaaatccg gtatatgggg atttatatct tgtaccgacg ccaattggaa
    23281 atttagaaga tatgagctac agaagtgttc gtatgctgca agaatccgat cttatcgcaa
    23341 gtgaggatac gcgtaataca caaaagttat tgaatcattt tgaaatccat acaccccaaa
    23401 aaagtttaca tgaacataat tataaagaac gtgtccctca attgattgct ttgttaaaag
    23461 aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc ttccatcagc gatcctggac
    23521 atgagctagt tttagcctgc ataaaagaag ggatttctgt gatctctatc ccaggaccta
    23581 cagctgggat gacggctttg attgcttcag ggctagtgcc tcaaccattt ttattttatg
    23641 gcttccttcc acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta aaagaacaga
    23701 cagttacgct gattttttat gagtcacctt atcggatcac tgctacattg acgaatttct
    23761 tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga attgacaaag gtccatgaag
    23821 aataccttag aggaagtacg gaagagctac tcgcttatat cgaagaacat ccagtaaagg
    23881 gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat gccagtgacg gaagttgatg
    23941 aagagttggg aacgttaaag gaacaagtag aagcaaagat cgcttcaggc gaaaaaccaa
    24001 atgctgctat caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa gtctaccgtc
    24061 agtaccatga attagactga gatagtataa gttgtctata atgaaagact aatgaggaaa
    24121 tcagctacta acttttcaaa tctcttgaac gcgaacgaat gttcttgtat aatgaataag
    24181 aggtgaggat gatggagctg caatttcctt tacataatga tacatcaaga aaaatcattc
    24241 atattgatat ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct gagtttcgcg
    24301 gacatccatt agtaattgcc agacacccca gtgacactgg tgggcgtgga gtagtgacta
    24361 cagcaaatta tgaagcaaga aaattcggaa ttcattcagc aatgagtgca caaaaagcgt
    24421 atgagctttg tccgcaagcc atctttaagc ctggcgacca tcataaatac agcgatattt
    24481 ccaaacaagt acgggaagta ttctatcgtt atacagatat tgtggaacca gttagtatcg
    24541 atgaagcgta tcttgatgtg accgaaaata aaatcaattg caagtcagcc atcaaaatcg
    24601 cccgaatgat ccaagcagat atttgggaga gtgtccagct aacatgttct gcgggagtaa
    24661 gttacaataa gtttttggcc aaattagcat cagactatca aaaacctaga ggaatcacgg
    24721 tagttgcccc tcaagacgct gtgccatttt taaaagcttt gccaattgaa aaatttcatg
    24781 gggtagggaa gaaaactgta cctagaatgc atgagctagg gatttataca ggaaaagacc
    24841 tatatgaatg cacagaaatg atgttgattc gaaattttgg aaaaatgggc tattctcttt
    24901 atcgtaaagt cagaggaatc catgattctc cagtaaacgt tacaagagag cgaaaatctg
    24961 tgggaaaaga gcatacttat gggcagccgt tgcaaacgga agaagcagtg ctgactcagt
    25021 tgcgccaact agctgaaaaa gtagaagaag cactgagaag agtccaaaaa catgggaaaa
    25081 cagtagtttt gaaggtcagg tacacggact attctactgt gaccaaaaga gtgacattgc
    25141 cagaatatat ctataaaaaa gaagcactct tttatcaagc aagccttata tgggaggaaa
    25201 tcttgggagt agaaaaagga atccgattgt tagggattac tttgacgaat ctggacccga
    25261 tgacttatga aaacatcgtc cttccattat gggaaaatca agaaatataa aaacagcttt
    25321 gagagttgaa accggaaatc cgttttctct cccaaagctg tttttatatc aagtcttgct
    25381 ttttgatttg ttcataggtt tctgttgcat ccgtacattt ttcccaaatc acaacttgat
    25441 tggattctaa agatttttgg acgtcaatga tccgctggtt gctacttccg cgaaattgta
    25501 gttttaggtc acgtttgctt aattcaaagc gtccgtcaac aaggatatcg atttgcgaaa
    25561 ggagttctaa tttatcttct gaatcttgta gcaattcatc aaaggtatag cctgtccatg
    25621 accagatatc ttttgtcgta ccgaactctt ttcggatacg tcgtacgact tgcagacaaa
    25681 catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg taatcgttgc
    25741 gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa tggtttgcca tatcggaaat
    25801 tttgtgcagc tttgttgaag cagccttcgc aggcaaataa gcagccacta acgtaaagac
    25861 tgttgcggac accttcacca tcgacaaaat taaacggttt gtagtcagca atatagtttt
    25921 ggctgtagtc ttttgctaac cattcttttg gttttggatt tctcataaaa aaacttcttt
    25981 catttaaggt tcaaagatat agctattgaa gataaaatct actcgaccac ataaataaga
    26041 acaaactgac ctaaatccat tgttcaagaa agcttgttct tattttgata gagggcgttc
    26101 aaagatacaa agccatcgaa agagggtggt gaaaaaagac ttgtcatagc ctcttattct
    26161 gaataaacgg tgttcaaaag cttgcatctg cagtaataag cccaactaaa cgaaaaatat
    26221 gaggaactat ttttgtttag ctattcttat tacttgatgc aggacagctt tttcacaacc
    26281 tcatttcatg tgtttcacac gtgaggaaat ttctttatgg cggccatgta ccatcggacg
    26341 agcttgcgga tttcctaaat agccacaagt acgtttgact acatcacatg tttttggatc
    26401 atgatttcca cattctggac attcgaaccc tcgttctgtc ggtttgaaat caccttcgaa
    26461 accacattga tagcaatgat cgattggcgt attcgtacca agatacccga ttttgtcata
    26521 tgcaaaatcc catacagctt ctaaagcttt cggattttgt tggagcatag ggtattcaca
    26581 gtagtggatg aatccacctg aacaatattt tggataatcc ttctcaaatt ccaatttttc
    26641 aaatggcgtt gggtttttac gcacatcata gtggaaactg tttgtataat attctttatc
    26701 tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac ggtcagttag
    26761 actttcactt ggagttgaat agacactgaa atgataacca tattcatccc cccatgcatc
    26821 tgcattagct tttaattctt tgacgatatc aagtgtgaaa gcttttgctt ctgggtttgt
    26881 ttcccattcg ccaccgaaga aagctgaagc aacttcatac aatccaatgt agcctaacga
    26941 tacagtagct cgacggtttt tgaataattc attgacagaa tcggtacgtg aaagacgttt
    27001 cccgaaagca ccatacatat ataaaattgg cgcatttgct ggaatggctt ctttacaacg
    27061 ttctacacga tagagaagcg catctttcat gatttctaag cggtcagaca gtaactgcca
    27121 gaatttattc tgatctcctt ttgattcgat cgcgattctt ggcaaattca atgtaacaac
    27181 acctaaattc attcgtccga catttacttc ttgaccattt tcgtctttcc acccttgcaa
    27241 gaatgaacga cagcccatag gtactttgaa acttccagtt aattcaacga ttttatcgta
    27301 attcagaata tcaggataca ttcttttcgt tgcacattcc aatgctagtt gtttgatgtc
    27361 gtaattagga tcttcagggt ttaagttcac accttttttc aaagtgaaga tcagtttagg
    27421 gaagatagct gtccgctttt cactccctaa tccattgatt cgaatctgta aaattgcttt
    27481 ttgaatttct ctttcaaacc agttttctcc tagtccaaat cctaatgaag tgaaaggagt
    27541 ttgtccattt gaagtaaata aggtattgat ttcatattcc aaactttgca tagcatcgaa
    27601 gatatctttt ttggtttttg cttttgcata ttctttttgg cgttctggcg aatcgatcca
    27661 ttcttccgcg tcttttagat gttttttata gttcaattct gcaaaaggag caagcaattc
    27721 atctgttcgg tcggcagaac agccaccata ttgacttgac gctacattgg caataatctg
    27781 tgagatttgt gctgtggcgg tctggataga ttttggcgat tcgacttcgg cattgccgat
    27841 tttgaaccca ttgttcagca tgcctttgaa atcaatcaag cagcaattcg tcatcggtgt
    27901 ataaggatga taatccaaat catggtaatg gatatcgcct ttttgatgcg catttgctat
    27961 atgtggtggc aacattttca gtccgattga ttttccaacg atcccggctg tcagatctcg
    28021 ctgtgtattg aaaacattac tgtctttatt tgcgttttcg ttcacgacag tttggtcctt
    28081 gttgattagc ttttcaatcg aaaagttgat atctgttgct ttactgcgag caaaatctcg
    28141 tttggtacga taattgatgt actcttctgc taattcatat tcgttttttt ctagtaatat
    28201 atgttccacg atgttttgaa tctcatagat tttgacattt tcagcaaacc ggcttttgat
    28261 ttcttggaca accaagtctg caatcatttg aattttttca taagtaagtg gtgataatga
    28321 gccgtgaatt ttcttttctg ctttgatcag tgcttcattg atcttttgtt catcgaaatc
    28381 aacatggcgg ccatcgcgtt tgatgacttt catctgggat gtctctggct gctgagtaac
    28441 gacagttttt tcttcataat ttactttcat tatcttcaac tcctgctaaa ttgttcttgt
    28501 ctatcataaa acaaaatcgg ggaaggagca actaaatatc ggctatatat agtattcaat
    28561 gtgctgtata attggatttc tatactatat gtagtgtata ttttattgtg aaaaaaggtg
    28621 aaaacttgta gctggtggat ttgttttttg catatttaat tttgctgaat aaaataattg
    28681 aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa cataagagca aattattcgt
    28741 agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag aagaattgga
    28801 aaagatgatc gaagtgaaac atctaagtaa aagctacggc agcaaacttt ctcccgtcaa
    28861 agccttagat aatatctcat tcacagtaga agaaggcgag tttgttggga ttatgggccc
    28921 aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt tgcctacgat
    28981 gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg agctcagtga
    29041 ctttcgtcga aaaaaactag gattcatttt tcaagagttc aatttgatcg ataccctaaa
    29101 tgcaaaagac aatatcctat tgcccctagc agttgaacgg atgactaaag aggaaatgga
    29161 aaagagagta agacatgtag ctcaattgtt gaatatagaa gaactgctca aacggtatcc
    29221 cgatgaactt tctgtcggac agagacaacg aattgcagct gctagagcac ttgttgttca
    29281 gccacaagtg atttttgcag atgaaccaac tggctctttg gactcgaagt cggctacaga
    29341 attgttgaac tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac tgttagtcac
    29401 ccatgatcca tatacagcaa gctactgcga tagaatttta tttattaaag atggtgtgat
    29461 tttttctgaa gttgtcagaa gagggacaag aagagagttc tttgagaaag taatagacat
    29521 gcaggcgacg attggcggag gaggaaaaat gaatgctgta taaactagcc gttaaaagtt
    29581 tttttcggca gtcacgaggc tatcttgttt attttttcag tttgacttta tccgtaatga
    29641 tctattattc cttcagcgca atgacctacg atcaatcttt agtcagaaga gcaagtcaag
    29701 atacttcgat agatggtgga ttgagttttg gtggatttat cattactgtt gttttgcttt
    29761 ttttcgtatt cagtgccaac cgatttttct tgaatagaag gcaaaaggaa ataggcatct
    29821 atcagctttt tggtatgaat aaactgcaga tatcaggtat atatgtgtta gaaatcatga
    29881 taattggatt gttcgcctgt atttctggga tattactcgg tattattttt tccaaactct
    29941 tttcaatgat tttggttcga atgatggata tggatcttac tagtccattt tttatctcca
    30001 ttccatcagt agtagatact ttatttgctt ttttcatcat ccttttagca gtatctgtat
    30061 attcaatatg gaaaatatgg cgctatccaa tgattcgaag ttttggagaa aaagaacaag
    30121 cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc atcgggttac
    30181 tacttttaac gagcggatat tttggagcta gtcattttag ggaaatcact tcatggatga
    30241 tcagttatca ttatcttgat acattagttt tcttttttcc ttttttaatt tttttcgttt
    30301 gtgtggtcgg tacttatttg tttttctgtt atacatttcg gttatttttg atcatattga
    30361 gtaaatggaa gatttattac tatggtatca attttttgat aatgggtaac acgcaagttc
    30421 atttgctcaa aagttggcgt acaaattctc tgattacagt cattctcgga atttctttaa
    30481 tgacaattgg cggcacggct agtatctcta ctattttatc ctaccaggag aaagtttcct
    30541 ccccgatgga ctatcagttt gatgttgaga cagagaaaaa aatcaagccg attctagcag
    30601 aggaagggca gaagataact aaagaaatca ccttgcatta taaagtcatt gggagtatct
    30661 atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa tctgatcacg gaaacacaat
    30721 atcaagcctt cagaaaagtt aatccagatt tgccgaacat ttctttgaaa tcacctggtc
    30781 atactgtttt gcttgatcaa atgcaaactc tttttcggaa tatttcatta tatggatcta
    30841 gtattggact gccagataat caagaattga agattcaaca aatgcttcca agtgtattag
    30901 gtgacgaaac aatggtatat ctaagtcctg tactgattgt agatgatacc gtttttgaaa
    30961 aagcaaaagg tgtggattat caagtagtca atgtagatgt atcaggtgga aataatgaaa
    31021 aaatagatga acgactgagc aatgacctgt cgataaagtg gctcaatgct gtttattatt
    31081 cttatgatat cgtaaatgga agtctacaag gagaaattag cacaaagaaa cttccggatg
    31141 accaaatgaa ggaagcacaa tttagccaag aaagctctcg tttgaatttc tcttccagct
    31201 attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc acactatttg
    31261 tcgggatgat cgtgattatt gctactggta gtattttgac agtccggcaa ttagcagaag
    31321 cagaggaaga aaaagaaaat tatcacctat tgactaagct ggggattcca caaagaagga
    31381 tcagacggat gatcttcgag caaaacgcac ttaccttctt tccacccatg atattaggga
    31441 tcactcacgc agtatttgca atcaatgtct tcactcagta tataaaaact gccgattact
    31501 ggttggctta tcttgtgagt ggcttattga tccttatcta tcttttgctt tatgtcatta
    31561 cgagtcagtt atattgtcgg attatcgagg aatgatatac ggaataagac aagataataa
    31621 atagaactaa attttgaata ccatttattt gga
//
LOCUS       DAJSVD010000024        31512 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSVD010000024 DAJSVD010000000
VERSION     DAJSVD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.024"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(35..478)
                     /locus_tag="ON498_000956"
     CDS             complement(35..478)
                     /locus_tag="ON498_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3939249.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(494..1654)
                     /locus_tag="ON498_000957"
     CDS             complement(494..1654)
                     /locus_tag="ON498_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3939250.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2161..3861
                     /locus_tag="ON498_000958"
     CDS             2161..3861
                     /locus_tag="ON498_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939251.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3963..4796)
                     /locus_tag="ON498_000959"
     CDS             complement(3963..4796)
                     /locus_tag="ON498_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3939252.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5100..7265)
                     /locus_tag="ON498_000960"
     CDS             complement(5100..7265)
                     /locus_tag="ON498_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3939253.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7525..9819
                     /locus_tag="ON498_000961"
     CDS             7525..9819
                     /locus_tag="ON498_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3939254.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9812..10477
                     /gene="pgmB"
                     /locus_tag="ON498_000962"
     CDS             9812..10477
                     /gene="pgmB"
                     /locus_tag="ON498_000962"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3939255.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10470..11477
                     /locus_tag="ON498_000963"
     CDS             10470..11477
                     /locus_tag="ON498_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3939256.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11499..12518
                     /locus_tag="ON498_000964"
     CDS             11499..12518
                     /locus_tag="ON498_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3939257.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12799..14166)
                     /gene="nhaC"
                     /locus_tag="ON498_000965"
     CDS             complement(12799..14166)
                     /gene="nhaC"
                     /locus_tag="ON498_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3939258.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14275..15696)
                     /locus_tag="ON498_000966"
     CDS             complement(14275..15696)
                     /locus_tag="ON498_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3939259.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15939..17816)
                     /gene="tdc"
                     /locus_tag="ON498_000967"
     CDS             complement(15939..17816)
                     /gene="tdc"
                     /locus_tag="ON498_000967"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3939260.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18107..19363)
                     /locus_tag="ON498_000968"
     CDS             complement(18107..19363)
                     /locus_tag="ON498_000968"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3939261.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(19475..19680)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19943..21244
                     /locus_tag="ON498_000969"
     CDS             19943..21244
                     /locus_tag="ON498_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3939262.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21313..21912)
                     /locus_tag="ON498_000970"
     CDS             complement(21313..21912)
                     /locus_tag="ON498_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3939263.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            22166..23047
                     /locus_tag="ON498_000971"
     CDS             22166..23047
                     /locus_tag="ON498_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939264.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            23060..24670
                     /locus_tag="ON498_000972"
     CDS             23060..24670
                     /locus_tag="ON498_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR3939265.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(24747..25406)
                     /locus_tag="ON498_000973"
     CDS             complement(24747..25406)
                     /locus_tag="ON498_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3939266.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(25689..26354)
                     /locus_tag="ON498_000974"
     CDS             complement(25689..26354)
                     /locus_tag="ON498_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR3939267.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(26501..27106)
                     /gene="dhaL"
                     /locus_tag="ON498_000975"
     CDS             complement(26501..27106)
                     /gene="dhaL"
                     /locus_tag="ON498_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR3939268.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(27134..28144)
                     /gene="dhaK"
                     /locus_tag="ON498_000976"
     CDS             complement(27134..28144)
                     /gene="dhaK"
                     /locus_tag="ON498_000976"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3939269.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(28119..28565)
                     /gene="dhaM"
                     /locus_tag="ON498_000977"
     CDS             complement(28119..28565)
                     /gene="dhaM"
                     /locus_tag="ON498_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR3939270.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            28668..28988
                     /locus_tag="ON498_000978"
     CDS             28668..28988
                     /locus_tag="ON498_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3939271.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(29100..29969)
                     /locus_tag="ON498_000979"
     CDS             complement(29100..29969)
                     /locus_tag="ON498_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3939272.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(30065..31369)
                     /locus_tag="ON498_000980"
     CDS             complement(30065..31369)
                     /locus_tag="ON498_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3939273.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(31366..>31512)
                     /locus_tag="ON498_000981"
     CDS             complement(31366..>31512)
                     /locus_tag="ON498_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939274.1"
                     /translation="ELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADLYKENG
                     VELK"
BASE COUNT         9191 a         6578 c         5717 g        10026 t
ORIGIN      
        1 ttaggctggg agcattattt cgttattctg ttcatcaaaa atcagcgttt tgattccatt
       61 tcatcacaag ttcctcctca tttactgctt catctttttg ctgtgtttga attgtaaaac
      121 cttgtgaaag atagaaatga atagctgatt gatttttttg atagactgtc aaagtcagtg
      181 ttttattggt ttcttttatt tttttcatta gttgcgtacc gattctttgt tgccggtatt
      241 cccttttcac aaagattcct gcgataaact gatcaatcac cccagcaaaa ccaataattt
      301 cttgatctat ttctgctaag taaatcactg catctggtaa agctttccta acagattcta
      361 cttgattctc ccaataagtt gatggaatga attcatgaga atccaagtta gcatgcagcc
      421 aaatcgatag aacagattcc agttctttgg cagacaattt tgttgttgtg cggatcattt
      481 tttctctcct tacttattca ttgattttaa aataaaaacc atttttttga agctctttta
      541 caagttttcg cccctcaaaa aagtatatct tctcttgttt tacttttcta ataccaaaag
      601 aaacagcaac tttaggatca tcagctgaaa cgattcgata agttccacca ggcaattgat
      661 ccagcttgac tttcccatga tggtcgcttt gtactgaaaa cggcacacca tttctagtaa
      721 gtgttttttt cttatgtttc aaaatgaatg aactaccatt taccggtttc cctgacgaat
      781 cactaataat aaatgaaaaa tcaagcactt ttttacttaa tctatactga tagattagtc
      841 gtccgataaa cactaacagt atgactacag ctattatcgt tcctgcaaga ataaaccatt
      901 gccgcatggt tctttcctga tttccttttc cttcttcttt ttttaccgat tcgttataag
      961 gaatccgatg tcctgtcact agcaaacgat gcgtgttgat catatacggc gtacatgtaa
     1021 gcaaggtaac taagtcttct ccttctgaaa tcgttagaac agacgtgtca tctggcagta
     1081 ccacttgtat atttttaact tgataagcca atttctcccc caaaacagtc aaaataaacg
     1141 tgtctccgtt ttctaaacga tctaattcgg taaataaccg tcgattaggt aaaccgctgt
     1201 gagcagagat cactgaatgc gtcgaatttc caccaagcgg gaaagaggtt ccttgcaaga
     1261 cgcctgctcc attttcaagg atcctaccat tgagccgatc ataaagtgaa atatttaatt
     1321 ggatcttagg tatattgata cttccgatca gccattcctc taattggctg gatgttgtac
     1381 tttcatttct gttcttttca ttgaaaggat cagtaccagg attgaatcct tggtcagtta
     1441 atcgttggtt tttctcctcc attttctttc gctgcacttc aatttcagct gcggtccgtt
     1501 tttgtacttg ttcgatacgt ttttgatcaa taaaactatt taaagaatcc acataaaatg
     1561 gatataaagc gatcagtaac cctacgacga tacatattaa taaaaacaaa cgaaacaaaa
     1621 gtcgacgaga ctgtttttgc ttactctgct tcacatgtgt cctcccttgc aagttttttt
     1681 gttctttctt ttattatatg ggttcaggaa aaaaatagaa aatgaaagcc gatgaggttg
     1741 tgaaaaagct gtcctgcatc aagtaataag tacagcccaa aaaaatagct actcatattt
     1801 tttgttgggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
     1861 ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta gtcagaaaat agcttctcat
     1921 attttctagg ctagtgagct tattaccgag aagcagctga ttgaacaccg tttaccgaga
     1981 ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa ttctgtttca aaacaaggag
     2041 atgcaacaaa acttatgtcg catcctctct ccttagaact ttcacttatg tttttcttgt
     2101 aaaattcata aatattttca tgaattttct aatttcttgc ctacctctcg cataggatat
     2161 atgatgaatt tatctaaagt ttgcaaagga gttcgacaat tgaaaaaata tggcccattt
     2221 ttagcaattt ttttcattgc tttggcattt ctcatgcctc atttattcac acataaacta
     2281 attttgggtg ctgatgcccc attccattac aatcggttct atgatgccgc gcaacaaatt
     2341 aaatatgggg atttccatta ttttccttcc ctttatggat tccagcaaag tggcaggatc
     2401 gtgaatgccg tttacggccc atttttcgct tatttacaag gattgatcgt gttgcttgca
     2461 ggtagctggt ttcattatca gattctttca aatttcattc tttatcttat ttcaggattt
     2521 tctatgctga ttttactgag atatgtcaat gttcgaaatt gggtaagtgt accagtagcc
     2581 gttttattca tgagtacgta ttctattcaa tactggtcta tcaaccaagg attcactagc
     2641 tgggggactt gtttttatcc tttatgtatg atccctattg ctgatttcgt ccttcataaa
     2701 aagttccctg ttatcaaagt cgctgtctca gtgggactta tgacacagat ccatttactg
     2761 tcttcgatca tgttagcttt gatgtatatc cctttttatc tttattactt ttttaaccag
     2821 actgaaaaag gaaaagcact gcttcaactg attaagtcgg tcttgcttta ttttgttctg
     2881 acagcaaata tttgggcagg attagctatt gtttacagtg ggaataagat tcttcctccc
     2941 tttgttaaca gagaaatgag tcaaaaggcg atagatctgg atggcagtta ttggctccat
     3001 taccctaagg cctttcctat attgatatta tcaggttttt tgctttttat tttctttcaa
     3061 caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa gtcttgtttt cttttttctt
     3121 tctactagct tgattccatg gacgacattg atcgaaaaaa atgtgccttt tattagtttg
     3181 atccagtttc catttcgatt tttcgtgcca tttacgctat tgcttctact ttaccttgcc
     3241 ctttcactga accagtggtc tgaacaaaag tggttccgtc ttttaagtat atttggcatt
     3301 cttatttttt cagcacagac gatccagaac ttataccagc atctggaaaa gtgggaaaat
     3361 gaaacatttg tcagtcgaca tacttatcta ttcgatacgc cggaagaagc aaggaaaaca
     3421 ttcttcagca aagatatgtc taccagcttg ttcgttttcc aaaaaaccac acctgactat
     3481 ctgccaatct ataaggagac aaaagacaat aaatacgacc ggtataacga agaaatacta
     3541 gaaaacgaaa caacttttat aaaaacacat ccaggcggtc ttttgacaat caagtggaaa
     3601 aacactgggg acaaaactgt tcatatccct gttatcgtct acgatcggac tgtgctacag
     3661 caaaatggaa aaacattgac tgattatgaa gtaacagata tcgggacccc aatcgtgaag
     3721 cagcaaaaag gaatcaacga gctcacgctt cattatcaaa cgccaatcta tttctatttc
     3781 attctttctc ttactttgat tggctggttt accttgctat gtttatttat ttatcataga
     3841 tacaagcttt tacgtgctta ggcaaaaggg ataaatacat ccgatcaaaa aactctccaa
     3901 acggtataat cgtttggaga gttttatttt aatcagtttt tttatgttat atgttatatt
     3961 ttttattcgt tcacatctaa ttcgatttcc accccgtagt gatcactgat gattggacta
     4021 tcgatcccat caaaaatccg ttgatatttt ttaacctgca ttccttcagg gacgaaaata
     4081 aaatctatac gcaatttttc tgtattttgt tcccaaccat ctattttctt ttccacagta
     4141 gctgcttttc ctttttcatt cgcgacaaca tacgaatctt gaagaagaag gcaactttct
     4201 cttacagtat aataaccttg agagtctact ggattattga aatcacctag gaaaaaaagt
     4261 ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc actcaaaata aaagccactt
     4321 tttttatctg tccaccaaga aaagtggcag ctcgagacaa aaatgtcctg atcatctatc
     4381 tttgttttcc caatcaagat cttgcgtgtc tgataatctt ctttcttttg aaattgggag
     4441 acggtaagga cctgactttc caatgttttt ttcgtaagca gtgcatttcc ttcttcatat
     4501 ttatcataac cgatgtggct catttcccaa ctccaaaaat actcttttcc gtgttccttt
     4561 aaaagctgaa ctaaacggta ggcaaaatta tctttacgga taggtacttg attctttacc
     4621 ggacagaact tcattagctt cgcaggttct acttcttcag attccagtaa ttggttgatt
     4681 tcttgtaaag caatcacttc ataatcttct aacaggatct tatctgctaa ttcttgcaat
     4741 tttttctctg gttctggctc aagccagcta tgcgtgttga gtgtcatgaa tttcattggt
     4801 tttctccttg tcttcatttc gaaacaaaag ggttgtgaca aaatttttgt cacaacctct
     4861 ccagcaaata gtgttctcct gctaactcct cagtaataag ctcaatggac caggagctat
     4921 tttttgtttg gctgttttta tcacttggtg cagggcagct ttttcacgac ctctccacta
     4981 aacagtattc aaactgctgc ttctcggtaa taagcccaac caaacgaaaa ataggaggag
     5041 ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctcct
     5101 tacattttaa tggagccgat cactttacca gctgtcactg tacctaattg cttgatcact
     5161 acttcttcta ctttatcgtt tgtaaatacg atcatgatcg ttggatcttt ttcttcgttt
     5221 tggatcacat caagatccat ctcagctaat ttcgttcctg ttcctacttc ttggccttct
     5281 ttgacagcaa tctcaaaagc tggctgattc atttggactg tatcgattcc catatggatc
     5341 aaaacttcga taccatcttt cgtttccagc ccaattgcat gtttgctagg gaaaatactt
     5401 gtcacttttc cagcgatcgg cgcaacaact tctcttgatg ttggaataac agcaaagccg
     5461 tcacccatca ttttttgtga gaataccgga ttttttactt cagataaagg aatcacttct
     5521 ccagaagctg cggaaactag ttcttgttct atacctaagt attgcttact acctgcttgt
     5581 ccatttcctg tagaaaccat ttctggctct ggaatctctg ctcctgattg cagcagatct
     5641 tcgatatctg atttcaagac atctgctttt ggtccataaa ctgcttggac tccattgtct
     5701 ttgacgatca atcccatcgc ccctgctcgt ttccaggctt cttcagtccc tactttttct
     5761 cgatcattta cacttacgcg taaccgagtc atacaagcat cgacatcttt gatattttct
     5821 tttcctccca gcaaatgaat gatctggacc acttgctgat ctgctgttcc tgttccgctg
     5881 ttagatacaa cggtttcttc actgtcattg tcgtaatttc cgttacgtcc cggtgtagca
     5941 aagttgaatt ttttgatcaa gaaatttgct aagaaatacg taacaacacc aaaagcgatc
     6001 acacacaaca cgaagttgat aagatctcca cctagacccg ctttgatagc taacggtgtc
     6061 cgagtcagca attcgatatt cccaaatgaa tggacccgca atggcaagat atctgccatc
     6121 gcaaatgcag ccccttggat cactgaataa atgacatata atggcacagc agcaaacata
     6181 aacatgaact ccaatggttc tgttacacct gttagaaaga cagccaacgc tgctgaaaaa
     6241 tacattgatt tatatcttgc tttcttatca ggatcgacat tacgatacat cgcaaaagcc
     6301 attcccatca agatacctga agaaccaatc atttgtccta ctttgaaacg agccggcgtc
     6361 cagttatgca acacaaactc atattgcgac atatcccctg cacctttaag attcactaaa
     6421 tcagtggccc aagccaacca taacggatct tgcccaaata cttgtgtccc cgcctgtgcg
     6481 cctgataaaa tctcatacga tccgccaagc tgtgtatagt tgataggaat cgtcagcata
     6541 tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc caaataaaaa cggtgctaga
     6601 atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa aattattgat tccagcctgt
     6661 acgtttggcc acaccacagc aagaatcaat gccacgattg ttgaccataa gataacaaca
     6721 aacggaacaa agcgctttcc attgaaaaat gacaaagcat ccggcaattt tctataatta
     6781 tagtacttat tataagccat tgccccgaca aatccagcga tgatccctac gaatactccc
     6841 atattcaaag ctggtgcttc caatacactt gtaaaaaatc ctttaaccat gatcttcgta
     6901 ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca tgtcacttgt gacaccaaag
     6961 atagccccag tgatccgatt gattaaaata aatgagatcc cagctgcaaa tgctccgcct
     7021 gctctttctt ttgcccaact tcctcctatt gctaaagcaa ataaaatatg aagattacca
     7081 ataattgccc aaccaatatt ttcaattact ccgcccgttg tgacaagcgg cgtccaatca
     7141 ggattgatca agggaattga tttccctata ctgatcatca acccagctgc cggcataact
     7201 gctaccacaa ccattaaagc ctttccaaat ttttgccaaa actcaaaact aaatatcttt
     7261 ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa acgtattata gcgtgcgttt
     7321 tcttttttgt aaatgcttta ttttttattt ttctgtctat accattctat aaaacctata
     7381 atacgtgtgt tcccttctat aattttctcc taaaaattta tttttttaag cttttttcta
     7441 tgaaatattt tcttttgtct attgacttgt gcaatcggtt gcgtttatta tgaacgtata
     7501 aaacagctca ttaggaggag aaaaatgaaa cagatcaaac gactatttgc tattgatcca
     7561 tggaaaatca gcacgacaca attagataaa aaaaatcttc gactgcaaga atctttaaca
     7621 agtattggaa acggttatat ggggatgcgc gggaactttg aagaaactta ctcaggggac
     7681 caccatcaag gtacttattt ggcgggtgtc tggtatcccg ataagacacg tgtcggctgg
     7741 tggaaaaatg gttatccaga atactttgga aaagtgatca acgccttgaa ctttatcgcg
     7801 atagatattt cgatcaacga tcagctgatc gacttggctt ctttggaacc agaagacttt
     7861 tactgggaat tagatatgaa aaatggtgta ttatctcgta cattcacaat cactacttct
     7921 actaataaag tacgcatttc cttcgaacgt tttctgagca ttgtcaaaaa agaatcagct
     7981 tatatctatt taactataga aatgcttgaa ggtagcggaa cagtcaaact cgtatcaaaa
     8041 ttggatggaa atgttcaaaa cgaagacagc aactatgatg aacatttttg gaatgagatt
     8101 gatcgtggaa ctatagaaga aatcggctat ttgacgacta aaactattcc aaacaacttt
     8161 gggattgaac aatttactgt tactgcagca atgcgccatt tcgttaatgg aaaatcagtc
     8221 atccctgact atcacgaaga tactttagca ttacatggtt gcttgtctgc agaattatct
     8281 caaggtgaga catggtcagt ggaaaaagaa attatcgttc ttactagtag agatattcca
     8341 gaagcgcagc aagcagaaaa agctgtctca gaattaacga accagacaaa caattatatc
     8401 caagcaaaag aagagcaaac agcggcttgg gcagaacgat ggaagcttgc cgatgtggtc
     8461 atcgaaggag acgaagaagc acaacaaggt attcgattca atttgttcca actattctct
     8521 acttactacg gcgaagatga acgattgaat atcggcccca aaggattcac aggagaaaaa
     8581 tacggcggag ccacttactg ggatacagaa gcgtatgctg tccctcttta tcttgcttta
     8641 gccaaacctg aagtaactaa gaatctgctg aaatatcggc ataatcaact gccgcaagca
     8701 atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt atcctatggt caccttcact
     8761 ggtgtcgaat gccataacga gtgggaaatc acattcgagg agattcaccg aaatggcgcg
     8821 attgcctatg caatctacaa ttatacgaat tacaccggcg atacggaata tttgaaaaat
     8881 gaaggactgg aagttttagc agaaatcgca cgcttctggg cagaccgtgt ccatttttca
     8941 aaacgagcaa ataaatacat gatccacggt gtgaccggcc caaacgaata tgagaacaat
     9001 atcaataaca attggtatac aaatacgatc gcagcttggg tcttgcgtta cacgcgagaa
     9061 aattatttaa cttatcaaaa agaagctgct atcactatca gcgaagaaga actagccaaa
     9121 tggaaagata tcgcagagaa catgtacttc ccagttgacg aagaattagg aatctttgtc
     9181 cagcatgaca cattcttaga taaagatctc atgcctgtat ccgatattcc cacaagcgat
     9241 cttcctctga atcagcattg gtcttgggac aaaatcctta gatcttgctt catcaagcag
     9301 gcagatgtac tacaggggat ttatttcttc aatgatactt ttacaaaaga agaaaagcaa
     9361 aaaaactttg atttctatga accaatgact gtccatgagt cttccctttc gccaagtatc
     9421 cattcgattt tagcagcaga attaggaatg gaaagcaaag ctgtagaaat gtaccagcga
     9481 acagctcgct tggatttgga taattacaat aacgacacag aagacggctt gcacatcact
     9541 tccatgactg gtagctggtt gtcgattgtc caaggatttg ctcaaatgaa aactgcccat
     9601 gatcagttga gattcgcacc atttctgcca aaaacttgga aagcttattc tttccatatc
     9661 aattatcgcg gacgtttatt gttcatctca gtcacagatg aacaagttac tttcgagtta
     9721 ttagaaggtt ctcctctaga aatttacgtc tatgaacagt cttattcttt agcggatcgt
     9781 ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa ggggtattgt ttgatttaga
     9841 cggtgtcatt accgatactg ctgaattcca ttatcgtgct tggcgaaagc ttggtcagga
     9901 aatcggcatt acgatcgacc acacattcaa cgaacagcta aaaggcgtca gccgagaaga
     9961 ctcactctct ttgcttttag cttatggcgg aaaagaacac tctttttcaa aggaagaatt
    10021 tgcagaatta gcgaaaagaa aaaacgatta ttatttagag atgatccaga cgatcgaacc
    10081 aaaagatgtc ttccccgggg tcgtgccttt actggattct ttaaaagaag aaaaaatcaa
    10141 gattgctctt gcttctgcaa gtaaaaacgg gccattccta cttgaaaaaa tgggtcttac
    10201 accttacttt gatgctattg ccgacccggc agaagcaaca aacggaaaac ctgcgccaga
    10261 tatcttcctc cttgcagcaa aagctgtcgg actaaaagca gaagaatgta tcgggatcga
    10321 agacgcgcaa gccggtatcc aagccatcct ctcaagtggc gctcaaccaa ttggcgtggg
    10381 acgtgctgaa gaccttggag aggagatccc gcttgtccct gatacgactt ttcttacgat
    10441 cgattacttg aaagaaaagt ggcatgacca tggataatcc agagatctcc gtcgtaccag
    10501 ttccagagac tgaaataaag aaaatcacat tgaagaacga ctacttagaa gtcgttcttc
    10561 tcaattacgg tgcccgcctg caccaaatct tcgcaccaga caaagaagga aaatcagaaa
    10621 atgttttatt gtcttacgat cgtttcgaag atgttctaac agacaaaagc ttttttggag
    10681 caactgtcgg acctgtagcc ggaaggatcc gggatgcttc ttggggtgag catagattgg
    10741 agaaaaactg cggatcacac catatccacg gtggcacgaa aggctggtct ttccaatttt
    10801 gggaagtaga agttttcaaa actccatcat caataggtgt ggtcttttac ttaaaagatg
    10861 aattttctac ctatccagga ccaatcactg ctacgatcac ttaccgttta acaaaaaacg
    10921 aattagagat gacaacctct gctagtagcc ttgttgaaac catctgcaat ccgaccaacc
    10981 atgcctactt caacctttca ggaaatggaa caagagacat ctatgaacat cagttaacgg
    11041 tctttttaga tggtgtgctt gaattagatc aagaaaaact gcctactgga aattggacaa
    11101 aaaaagaaaa actgccaatc gactttagaa aatccccaac actgcaagag atccttgcat
    11161 gttatccaga tggactagat gatgtatttc ttttgcatca tcctaggctt tccaaaactt
    11221 ccttacaatt attcgagaag cattctggtc gccaaatgac tatagcaact tctaataaaa
    11281 gtatggtatt attttctacg actggattcg aagctgattt ctctgtcaat ggaaaacaga
    11341 tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat ccctgatatc gttcatttcc
    11401 ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg gatcagtcag acgatttatc
    11461 ggttctctgc acaataacaa gagaaaggtg tttttatcat ggctattacc gtaaaagatg
    11521 tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg tgtcatcaac gatcatccta
    11581 gtatttccga gacaacaaag aagaaagtcc gaaaaattat ggatgaacta ggttatgttc
    11641 ctaacatgac tgcccgcaat ctaggaaaac gcatttccag tgccatcggc gtaatcttac
    11701 cccctcttga ttcaaaagag cggatcggta acccatttta tcttgagatc atggaagcaa
    11761 tcaacgaaga agcgcggaat tatgatatga ctacagcaat cgctactgct aaaaattttg
    11821 aattgctgtt agagaacgtc cagcgtatgc atttacaaaa acaagtagat ggttttattc
    11881 tagtgtattc agatagtcaa gatactgtca ttgattattt ataccaaaac cacatccctt
    11941 tcactttgat cggacagcct tatcatcatg agaacgaaat cgtttatgta gataatgata
    12001 accagcttct aggtaaacaa gcaacagaat ttttgatcca gaatggacat aagaatatcc
    12061 tgtttgtgac aaacacgact catgaaagtt tgtatttcga acgatatttc ggttatcaaa
    12121 aagcaatgat gatggctggt cttcctgcgt tatccagtgt agtactagag catccggagg
    12181 attatctgtc ctttgagaaa acgttgaaag aaaacgaagc cacagcattg gtggtcattg
    12241 acgatatctt cgcccttcgc acgatgcagt tggcgcaaat gtatggctat gtcattccag
    12301 agacactctc cgtcattagc ttcaacaact cgatcttctc aacattaaca cacccttacc
    12361 ttacaagtat cgatatcgat atctccgaat taggacgtgt cgctatgcaa aagctgcgcg
    12421 aattgatcca tgaagaattg agcagtggcg tacgtttggt catccctcat cgcttgatca
    12481 aaagagagac agtcgttcct ttagaaaacc catactaaat agctgactca ctaaatacgc
    12541 tgaccattta tcctatggtc agctttttat gtaaaagaag atactacttc taacacattg
    12601 tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    12661 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    12721 tttaaaaagc aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca
    12781 gcctcttttt tccttccttc aattttttac acgtcgacga ttcaataaaa aaccgcctaa
    12841 tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct
    12901 aactttcatc gttcccataa tgaatgtgcc gctgactccc cacggtacga gagaattgac
    12961 ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt tttgataagc caacgtcatc
    13021 aaaagaacgc ttgaatgttt ctcctggaag aatgatggac agatactgtt ctccaacgat
    13081 cagattgatt ccgatacagc tcaaggcagt aaacccgact aaacgagaag gtcggtccat
    13141 cttttctttt aattcttgaa cgatggtatc aacgatcaga tatttgatca gtaacccacc
    13201 taatgccaat gcaagtaaaa tcaaactagc agaacctaac atactcatga tcccgcctcg
    13261 agaaagcagt tcatctagct ttggatcagg tgtatttgcg acataaccat tcatcaagat
    13321 gtctgctgct tttccgaccg tcaaactttt ctcatggata aaagaaagaa cgagagcagc
    13381 tgtcgatccc gctagtaatg tagggatagc cggaattttt ttgattgccc ccccaaataa
    13441 aataactagt ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat
    13501 catttcattg atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa
    13561 aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa
    13621 tagattaact cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt
    13681 attccctaaa aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag
    13741 taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc
    13801 gacagtcact ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga
    13861 aacaagttta aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc
    13921 aactaagaca ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt
    13981 gatgatcccg tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa
    14041 acacatgaaa gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac
    14101 tcctaggagt gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt
    14161 attcatcgtc gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt
    14221 ggcaggacct cctactaaat cagcgacaaa acgtcggctg aaagacttat ttttttattt
    14281 ttcttctaaa cttttttcta cctcaatccc ccaagaatct ttcttgaagt gattgatgat
    14341 caatggaata acgaacatga ccactgtgat agctgctact aataatgcat cactcaatgt
    14401 cgctgtacct aatgtagcaa atacgacaac gatgatagag aagatcagca tgcagctgac
    14461 tacccaagct aatccgtttc cgtttttacc aattcggtaa ggacgttcga attctggatg
    14521 tttcttacgc attttaagca atgaaacagc aatcaaaatg tacacgatac aataaataac
    14581 agttgtagca tttgtaagtg tcaagaatac agcacttacg tcatccatta caccataaag
    14641 caaagcaaat aatgaaataa cgattgattg tgttagaacg acatttctag atacgccgta
    14701 tttgttttct ttatggaatc cgaattttgg tggaaggaat ccttcacgag ctacttggat
    14761 gattgttttt gatggtccag taacccaagc tgataattgt aaaagcacac caataaatac
    14821 catgaagctg aagatatttc cgatcacttc tggtaagcct aggatttgac agtcaatcaa
    14881 gataggctgc gtgatatttg ctaattccat ttttccatct ggtacgacat ttgctacaag
    14941 catcgcattg atgacattca ataaaactaa tccgatcaaa gcgataaata cgccttttgt
    15001 ataatttttt gttgcgtctt tcaaacgagg catgtaaaca gaactcattt cgatcccaac
    15061 aaaaataaat gtgataccag ctaagtattt caatgaatca atatgttcca agtcagggaa
    15121 agcttttgac catgaaaagt cacctagata gccgtttgag actagtccga ctttgaaggc
    15181 tgccaaaaca cctaaaacga acatgatgac tactgggata tataccccta gccatacacc
    15241 gatgttcccg ccgacttttg ccatatcgaa tttcaagttc aagatcgtga tgatccagta
    15301 gataactaaa atacaaccta aaacgaacca gtgattattg ccaagttcta cattaccaaa
    15361 tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt accatacctg ggaacatttg
    15421 cacccataat agccatgcaa cgacaaatcc ccatttgctg cccagtgctg tttttaccca
    15481 taactgagga ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa ccattgatat
    15541 cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc
    15601 ggctaacgta ggaatactcc gaaccgttgc acacagtgcc attgtcagac cgataaaggt
    15661 cccaagaccc attttactta cttcagattt actcaccgtt gacactctcc ttgatttttt
    15721 ggataaaata acggtgtctt tgaagtagtt tcagcaataa acgaaacaat tcagcgaata
    15781 tttgagggga ttctttgagt ttattgcttg aagggtttct tcaaagacaa cctcaaaaca
    15841 aagggatcga agagcttagg tctccccttg ctcttcagat ccaaacatgc gtcagaaaca
    15901 gttagtattc aagctgtttt ctaacatgat taccttgctt attttgcttc gcttgccaat
    15961 aattggtctg cgtagatttt ttccaatttt tcttgaagag ctgctttgat tttttcagca
    16021 tattcttcaa agtgttcttc tttgttcatg tatggtgtca ttacgctggc acgcaataca
    16081 gttacttttc cggcacggtt ccattcttca tctgagaatc ctagttggtt cacaaattgt
    16141 aatgggctgt tgccatagtc tgggatagca aagtcagtat gtgaagttaa gaactcatta
    16201 ccgtaaatgc ttcctttcac gtatgaagaa taatcatata cgtcatggtt caatttattc
    16261 attgcaacta agtcatcgtt gcctttttct ttgaatacat agtctaccat attgaaatct
    16321 ggataagtta atggatgaac ttcgatttct ttgtctccta ctttaaatga caagtcattc
    16381 aagaagttgt agaaacgatg tgcaccttcg attgatgcac ccattaattt accatatcct
    16441 gtgacattca aaggtaagac atggtgtgct gcccaaacgc tggctgctgt tgcccctgct
    16501 tttgaacctt ccaagatata agccccaagt aatgctggga tatctgcgcc tttttcaaat
    16561 acatatgtcg caaagtaaga gataacgttt ctcatgcgga tatctttgat gacgatacca
    16621 ccggcagaat atggaacata gcccatttta tgtgggtcaa tcgttacaga ttctgcttct
    16681 tcgattgctt tatatgcact gtgtacttct tctaagatat aatctttgtt ttctgtaaag
    16741 acattgtatt tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg
    16801 tctaagaaaa tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa
    16861 ataccgtctt tttctaatac gcgacgcaat gcaacgattt tatcgatacc gtcgattgct
    16921 ccttcttctg ttgatcctac aacaccgact acacctagga ttggtgtttt ttcagctgct
    16981 agaccacgaa cgattttttc aagttcgttg atgtccatgc gatagttgtg gtcaactggc
    17041 actggaatca cttggtctaa accgataccg atgatgtcgg cagcttttag ccatgaatag
    17101 tgtttagttt gtggaaccaa ccattttcct aatttttcta aatgttttcc gctacgtgct
    17161 gagtgagctt tgatttcgtc gattttttct gggacgctgt ctaacagatt catgatttct
    17221 tctgttgaca agttcattaa ttcccaatcg ctttttccag caactagttc aggtgtcact
    17281 tctttcattg caagcggcaa tgatttgatg ttgcgggcat accaaagtcc ttctaagttt
    17341 gctaaagaac catcagcaac gatgtggccc cagccatctt tatagctcat taattttgca
    17401 aattccattc ctacttcttc ttccatttgg cttgttgcag gagaagattc ataggcaacg
    17461 ttattaccat tccatagcat agcaaaattg tacgctagta aagatggcat caatgtttca
    17521 gagttcatat gaccccagta acgtccggca ttatgccaag gaacggaatg ggtacgcatc
    17581 cgcgctgaaa tttctgatag aacatccttt gttttattca cagtgtgttc aaaactagca
    17641 ctgctttttt cttctggcgt aataataggc atgtcttgag gcatgtagtt ttgacgccag
    17701 cctaaatgtt catccaccaa ttcattcaac aaagctttat agatttggcc gttctcagct
    17761 ttgtccccaa tgaatagggc atttaggttt agatctttcg acaatgattc actcatagtg
    17821 aaaccctcct aaaaaaatat ttaaattttg tcgagcccta cattacacct gttcttctat
    17881 tctgtaaact aagttttggc ctgattttta atcatatttt aatatgtatc aattatccga
    17941 attgttcaca cttttgaaat aattaaaaaa aacgagggaa tttacaaatg taaactccct
    18001 cgcaaagatt gtattaacga caaccagcgt tctattttag gatgggtttc aaaattggaa
    18061 tgttgtaata gtatttccct ttttcaaggt aaaaacttac taaattctaa tcaattactt
    18121 tggccaaaaa gtaatttttc ttaccttttc gaataaccac aaacttacca tcgaattcat
    18181 cggatggatt tatgacaaaa tttaaatcgg taacgcggtc accgttaata cttatagctc
    18241 cgtttgaaac atcttcacgt gcttgacgtt tagatggttc aatttttgtg gaaacaagca
    18301 gttccacgat attttcaggt gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt
    18361 gcgcgatttc ttccgcattt agttctttaa tatttccaga gaacaatgct tgcgttattt
    18421 tttgcgcttc tttcaagtct tcttcactat gcacaaatct tgtaacttct tctgccaaac
    18481 ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt tttcgctaag tcttcgattt
    18541 cttcttgaga caagaaagta aagaatttca agtatttcac tacatcgcga tcatcttgat
    18601 tcaaccagaa ttggtagaac tcaaatggtg aagttttctt cggatcaagc caaacggcac
    18661 cgcctgcagt ttttccgaat ttcgttccgt cagccttcag catcaatggg atcgttaaac
    18721 cgaacgcttc tgcttctggt ccttctaatt ttcggattag gtctaatcca gctgtgatat
    18781 ttccccattg gtctgctcca ccgatttgca attgcacatc gtatgtttta tgcagatgca
    18841 aaaagtcgat agattgcagg atttggtaag taaactctgt aaatgagatt cctacttcca
    18901 aacggctggc aacgatatct tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt
    18961 ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc gtagttgttc acaaatgtga
    19021 tatttgcatc tttaccaaac aattttctca tttgattgga aagtgcgtcc acgttatgtt
    19081 gcacttgttc catcgtttgc aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg
    19141 ttcctgttcc gccgccaatc agaatatatg gatgatggcc tgccaattgg aatcttttca
    19201 tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc accagttgga tcaacgccgc
    19261 aataaagtga aatgctcttt tcttcaacga gctcgcgaag tctttcttcg tttgtttgct
    19321 gattaatggc gtcgcgccaa gttaattcat caataatatt cattttactg acctccgtgt
    19381 agttttttta tgaaagcagg aacctgctag catatgtaat taattatttc ttcaggatag
    19441 gataggaaat aaaaaaagtc ccttggcata taagccaagg gacgaaatct cgcggtacca
    19501 cccaatttgt atgtctagat atccaacata cctctcgaac atttaacgct gtcgcgtggc
    19561 aatcactgcc aaagactcct gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg
    19621 accaccagct ttctgaaaca ttggctttaa caactacttc gtttcgatct tcgtcttgtt
    19681 attattaata tgtgtctatc atagagcgca actttttctt tgtcaacaga aaaaggatat
    19741 tttttttaaa ttcctcattt tttctttttt gaatccgctc taattccgaa cattttattt
    19801 tcaattattg ttaaaagaaa taactttttc tgtatttttt ctgcaattta tcgagaagtc
    19861 actttacttt gcctcttatt ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat
    19921 gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa attattttag gagccacttc
    19981 tgctttaact atcgttattt taattctaag tttgtttttt gtcatccgat catctacaaa
    20041 aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga
    20101 taagcaacaa ctagttactc aagcaaaaaa tctggctcaa agctacgatt atgaccaagc
    20161 gattgcgtta ttggaaaaag atagtaagaa cggatctact gaaacacaac agcttcttca
    20221 aacactaaaa aaggagaaag aacaactgat cgactggtct gatcctactc agatctctca
    20281 tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca
    20341 aggatacaaa gattatatgg tgactgttga agaattcaac cgttcgatcg aacaacttta
    20401 tcaaaatgat tttgttttag ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa
    20461 tctttccttt aacgggcttg cgctacctga aggaaaaaaa ccattggttc tttcacaaga
    20521 tgatgtcagc tattatgaat atatggataa ttccggtttt cctagccgat tggtcgtatc
    20581 aaataacaat gaaatcaaaa atgtctataa agagaaagat aaagaagaaa tcggtgactt
    20641 tgacatggtt cctctgatcg atgcctttgt caagaaacat cctgattttt cttatcgagg
    20701 cgcaaaaggt accttggccc tgacaggtta caacggagtt ttaggttatc gaacatcaaa
    20761 atctcaatat ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc
    20821 ggaacagttg aagaaagacg gctggagctt tgcttcccat acatggggtc acttgaatat
    20881 gactcaagct tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc
    20941 acctatccta ggaaaaacaa atattctgat ttatccattt ggtgcagaca tcagcgactg
    21001 gcagccttat tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag gtttcgatat
    21061 tttctgtaat gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg atttctatcg
    21121 aaatgctcgg atcaatatcg atggcattcg ttttcaatct gatttagacg gaaaaaatcc
    21181 tattcttgat ccattcatca atgtccatga tgtctatgac aaagaagcac gcgaatctgc
    21241 ttgattttac gtcatttatg aaccaaaaaa gccgaactga cattttatcg tcagtttcgg
    21301 cttttttatg ctttatttct tcttcggtgc gattcccttg atcaggactt ctgtccattc
    21361 tgctgtatat ggaagcagtt ttgcgtatat ctccggctta gaaattcctt ctaccattgc
    21421 ttgaaaatag aggatcaaga actcatccga atatttcaaa tccacttttc cttctttccg
    21481 ccctcgatgg aaaagatcaa gcatgatact gaaactttct tgcgtatacg catccatcgc
    21541 gtcagaaaat tctttttttt ctttttttgt atagtatgtc atcaaatcga tataaaattg
    21601 tttgttcact cgattgagat aacggatttt gttttcactc atggcaatca gtgtttcttc
    21661 aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc
    21721 aaaaaatact tcataaatca gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt
    21781 acttacctga gcgttctcag ctaccttatc cattgtcact ccagcggttc ctatgtctgt
    21841 gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc
    21901 aaaaccgttc atttgtagag cctcccgtaa ttttaaactt ttataatata tatagttcaa
    21961 atttattctg aggtttatta taactatcta ttgagaaagt atcaagctag caattccttg
    22021 tttatgaatt cattgacaga aatttataaa aagaatatta tgtactaaaa taataaaaaa
    22081 agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt tagtatatat
    22141 ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa
    22201 gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt
    22261 cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg
    22321 gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag
    22381 tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt
    22441 gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa
    22501 aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc
    22561 aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta
    22621 tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga
    22681 agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga
    22741 agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg atgaagaagg
    22801 aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga
    22861 gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc
    22921 tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg
    22981 tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga
    23041 aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca
    23101 aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc
    23161 tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata
    23221 cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc
    23281 tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt
    23341 tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg
    23401 tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat
    23461 cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt
    23521 tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat ctgtaggtag
    23581 tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc
    23641 cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac
    23701 agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta
    23761 tccatttaca actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt
    23821 gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga
    23881 acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta
    23941 tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata
    24001 cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat
    24061 gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca tggtagtctt
    24121 gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac
    24181 ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac
    24241 caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact
    24301 agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct
    24361 taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct
    24421 attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt actcctttgc
    24481 actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc
    24541 tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc
    24601 atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact
    24661 agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat
    24721 ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc
    24781 cagacttctc gattctttct ttttacataa ccattctcct ctaataattt aaattttcta
    24841 gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg
    24901 aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct
    24961 tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg
    25021 ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct
    25081 ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt
    25141 ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc
    25201 tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact
    25261 aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg
    25321 acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat
    25381 gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata
    25441 aagaaacata acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca
    25501 agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt
    25561 cttttgagac cttaagtaat tgattcctat caatttttta aatatttact ttttgaataa
    25621 tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca ttgcttattt
    25681 cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc tagttcttgt
    25741 aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa gaatagttta
    25801 cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt ctttgtatcg
    25861 gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg cggccatatg
    25921 taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag caactggtta
    25981 tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat caaatagatt
    26041 tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg acgagattcg
    26101 cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca gctttcgatt
    26161 ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag ctgaatctct
    26221 ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt atctgttgga
    26281 tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg ataatctgat
    26341 tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc tgaattttgc
    26401 gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa gaacagccaa
    26461 acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga ttgatcacat
    26521 ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata tgtccgattg
    26581 cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg gtgtgttctt
    26641 ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct gtgactggca
    26701 gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt tgaatacccg
    26761 ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa tcgacctctt
    26821 ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa gccccaccga
    26881 ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt tcaggatgat
    26941 cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt ccatggtctc
    27001 catcgccaat cggggtatct agatcactga gatattgttt gttctgattg attgcctctg
    27061 caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta tcaccctttt
    27121 atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa tctttccatt
    27181 gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt gaggtcataa
    27241 aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga gctaaaacat
    27301 cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg actagtacac
    27361 ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt tcctttgcca
    27421 gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg ataccaacac
    27481 catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct ggtacagttg
    27541 ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct cccagttctt
    27601 tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga
    27661 cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg gcgatatcat
    27721 catctacgat cacactttct acttcaatat cgtccatagc agctaattcc tttgccatat
    27781 cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct tttccactat
    27841 cggcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac acatcaccaa
    27901 gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt tcatgtccgc
    27961 ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct acaacacggg
    28021 aagattctac acgatgaaca agttctggat agctttttac taatccttcc agcatttctt
    28081 cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc tttctttccg
    28141 gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc agtgatggct
    28201 gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg ctgctgctct
    28261 tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat atccgcaaaa
    28321 acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt catcgggtct
    28381 gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc ggatttttgc
    28441 atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga aataagcaaa
    28501 atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat tggtttcgct
    28561 ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaaa tatgtgtagt
    28621 gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg attacaacaa
    28681 caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt tttggtgagg
    28741 tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat atctttggcg
    28801 gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca cttgccgaaa
    28861 tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa atggactatg
    28921 aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca gctgtctcca
    28981 cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat gtagtggcac
    29041 tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc ctctcttttt
    29101 tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc ccctgctaag
    29161 gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact tgttgatttg
    29221 gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac tgatttttct
    29281 cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc agcttttttt
    29341 gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat tgcttgactg
    29401 gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg agcaacctga
    29461 tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca
    29521 gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc
    29581 actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc tttcaatttg
    29641 ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa
    29701 tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga agatgaaatc
    29761 gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga ggcaccgttt
    29821 tcagcaccag aagagcttat ggttgttgaa ggttctgttt gagcaggttc agaagtttcc
    29881 gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca gattaacggc
    29941 ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat ttcttattat
    30001 aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa aaacggtgca
    30061 tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa cctaaaatcg
    30121 ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa
    30181 gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca tctacaatca
    30241 gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac acaaaactga
    30301 atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc ccgattaatc
    30361 cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc aatgtttgat
    30421 caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact tttttgaaat
    30481 tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctgtgtcga tatagcatct
    30541 tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca gataaccagt
    30601 catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct tgtctgaatc
    30661 cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg tacgcataaa
    30721 gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt gagaattcaa
    30781 ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt gcagtgaccg
    30841 cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc agtgcatctg
    30901 gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca aaggcaagtc
    30961 cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca cgaagattag
    31021 cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg
    31081 tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg attgtatatt
    31141 cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt tcgacactca
    31201 agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa caccagacac
    31261 caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaaa tacataatag
    31321 acaaacaagt atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac
    31381 cccattttct ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac
    31441 agaataaaaa cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc
    31501 ttcaagtaat tc
//
LOCUS       DAJSVD010000025        29813 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSVD010000025 DAJSVD010000000
VERSION     DAJSVD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.025"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(273..752)
                     /gene="rlmH"
                     /locus_tag="ON498_000982"
     CDS             complement(273..752)
                     /gene="rlmH"
                     /locus_tag="ON498_000982"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3939275.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1180..2469
                     /locus_tag="ON498_000983"
     CDS             1180..2469
                     /locus_tag="ON498_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3939276.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2508..3113)
                     /locus_tag="ON498_000984"
     CDS             complement(2508..3113)
                     /locus_tag="ON498_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR3939277.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3318..3782)
                     /locus_tag="ON498_000985"
     CDS             complement(3318..3782)
                     /locus_tag="ON498_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3939278.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3804..4124)
                     /locus_tag="ON498_000986"
     CDS             complement(3804..4124)
                     /locus_tag="ON498_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3939279.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4268..5347)
                     /gene="pepA"
                     /locus_tag="ON498_000987"
     CDS             complement(4268..5347)
                     /gene="pepA"
                     /locus_tag="ON498_000987"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR3939280.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5547..5897
                     /locus_tag="ON498_000988"
     CDS             5547..5897
                     /locus_tag="ON498_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3939281.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6120..7067
                     /locus_tag="ON498_000989"
     CDS             6120..7067
                     /locus_tag="ON498_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3939282.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7124..8542)
                     /gene="pepV"
                     /locus_tag="ON498_000990"
     CDS             complement(7124..8542)
                     /gene="pepV"
                     /locus_tag="ON498_000990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR3939283.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8691..9536
                     /locus_tag="ON498_000991"
     CDS             8691..9536
                     /locus_tag="ON498_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR3939284.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            9540..9980
                     /locus_tag="ON498_000992"
     CDS             9540..9980
                     /locus_tag="ON498_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3939285.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10030..10224
                     /locus_tag="ON498_000993"
     CDS             10030..10224
                     /locus_tag="ON498_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939286.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10360..11046
                     /locus_tag="ON498_000994"
                     /pseudo
     CDS             10360..11046
                     /locus_tag="ON498_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(11454..12143)
                     /locus_tag="ON498_000995"
     CDS             complement(11454..12143)
                     /locus_tag="ON498_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939287.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12118..14247)
                     /locus_tag="ON498_000996"
     CDS             complement(12118..14247)
                     /locus_tag="ON498_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR3939288.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14295..15632)
                     /locus_tag="ON498_000997"
     CDS             complement(14295..15632)
                     /locus_tag="ON498_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR3939289.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15884..16243)
                     /gene="rplT"
                     /locus_tag="ON498_000998"
     CDS             complement(15884..16243)
                     /gene="rplT"
                     /locus_tag="ON498_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3939290.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16296..16496)
                     /gene="rpmI"
                     /locus_tag="ON498_000999"
     CDS             complement(16296..16496)
                     /gene="rpmI"
                     /locus_tag="ON498_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3939291.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16568..17092)
                     /gene="infC"
                     /locus_tag="ON498_001000"
     CDS             complement(16568..17092)
                     /gene="infC"
                     /locus_tag="ON498_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3939292.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17136..17261)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17412..18044
                     /locus_tag="ON498_001001"
     CDS             17412..18044
                     /locus_tag="ON498_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3939293.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18046..18951)
                     /locus_tag="ON498_001002"
     CDS             complement(18046..18951)
                     /locus_tag="ON498_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3939294.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19036..19695)
                     /locus_tag="ON498_001003"
     CDS             complement(19036..19695)
                     /locus_tag="ON498_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3939295.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20011..20955
                     /gene="mvk"
                     /locus_tag="ON498_001004"
     CDS             20011..20955
                     /gene="mvk"
                     /locus_tag="ON498_001004"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR3939296.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            20948..21925
                     /gene="mvaD"
                     /locus_tag="ON498_001005"
     CDS             20948..21925
                     /gene="mvaD"
                     /locus_tag="ON498_001005"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR3939297.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            21938..23023
                     /locus_tag="ON498_001006"
     CDS             21938..23023
                     /locus_tag="ON498_001006"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR3939298.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23020..24084
                     /locus_tag="ON498_001007"
     CDS             23020..24084
                     /locus_tag="ON498_001007"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3939299.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24333..25136)
                     /locus_tag="ON498_001008"
     CDS             complement(24333..25136)
                     /locus_tag="ON498_001008"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR3939300.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25156..25998)
                     /gene="kduI"
                     /locus_tag="ON498_001009"
     CDS             complement(25156..25998)
                     /gene="kduI"
                     /locus_tag="ON498_001009"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR3939301.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26014..27021)
                     /locus_tag="ON498_001010"
     CDS             complement(26014..27021)
                     /locus_tag="ON498_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3939302.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27028..27672)
                     /locus_tag="ON498_001011"
     CDS             complement(27028..27672)
                     /locus_tag="ON498_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3939303.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27772..28530)
                     /locus_tag="ON498_001012"
     CDS             complement(27772..28530)
                     /locus_tag="ON498_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3939304.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28657..29808)
                     /gene="fucO"
                     /locus_tag="ON498_001013"
     CDS             complement(28657..29808)
                     /gene="fucO"
                     /locus_tag="ON498_001013"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR3939305.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         8630 a         6235 c         5589 g         9359 t
ORIGIN      
        1 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
       61 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
      121 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
      181 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
      241 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc ccccgcattg
      301 atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg atgtggatac
      361 gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc gctgagtcct
      421 aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa ttggtcgatt
      481 tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc atagacgtat
      541 tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac ttgtctcatt
      601 tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa ttcgattttt
      661 gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta tttttctttt
      721 aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc ttcatgctca
      781 ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt tttgttaaaa
      841 agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa cagtgttatt
      901 ttaccttatt tcatcacatt cattaggcaa acatacgttt tataagaatt tatccacttt
      961 atacacaggc tgtggatatg ttttatccct tatttttcca atcctatccg cgtagtttta
     1021 cacagtatct gtggaaaaag aaaatcaaat attttcatct gaaaacaatg atttttccta
     1081 aagtttttca taaaaagttc acagtctcaa agtagactaa agtcaacaaa taagactata
     1141 ctaacagtag ataaatgaat gtaattggag gaggaacaca tggatagaaa aaatgtgaca
     1201 ccaaagatga aaaaaagtaa aaataatagc ctctggcgta agttaggtct ggggcttgtt
     1261 ggagggatcg ttggaggact actgacagct ggcatttttt atgcagcgat gggcactggc
     1321 aacaatagtt ctactgctac aagcggcaat cagaattctg ccggtgaaac agttgtggaa
     1381 aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg tagataaagt tcaagacgct
     1441 gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa gcggttttgg cggactattc
     1501 gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta tagtgaagga
     1561 agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacgaa taatcacgta
     1621 gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt aaaagctgaa
     1681 ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc agacaaagtc
     1741 gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc tgcgattgcg
     1801 attggttccc cactaggatc tgaatatgca aattctgtca cttcaggaat catttcttcc
     1861 ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa cagtcaatat caatgcgatc
     1921 caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagttaa tatcgaagga
     1981 caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac ttctaatgta
     2041 agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat catcaatcaa
     2101 ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt cgacttatct
     2161 gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt aactaacggt
     2221 gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt gaaacaatac
     2281 gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact tcaatctgct
     2341 ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg ctcgaaagaa
     2401 aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa tcaaaatagt
     2461 gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac aacctcttta ttttggaaat
     2521 gcttctccga ctacggcatc ctcaggcaat actaagattc ctttttttgc tggagcattt
     2581 ggcaaatgca attcacgagc ggaacagatc attccatagc tatctacccc tcgcaaagtt
     2641 cctggccaga tcatcattcc atctggcatc atagctcctg gttttgcaac gacaactttt
     2701 tgtccagctt cgatattggg tgcaccgcaa acgatttgca atactgtacc attgtctact
     2761 tctgtctcag taatcgataa atggtcagaa tcaggatgtg ggacgcatgt ttttacgtac
     2821 ccgacaacga tcttcggttc atgatcagct tcaagctgtt cttcgaatcc tgcttctttg
     2881 atcaatcgat tcaatttcgc taattcctca tctgttaagc ttacttggcc gttcccttcg
     2941 attgttagat gatcggagat atggaaaaag ttccaagcaa caacttgacc ttgttcatct
     3001 ttgatccgag caacattcca tttgcgttca acagtgtttt ctgttccttt atcgtcggct
     3061 ataataacca tcaaggtgtc tccgacgtgt gctttgttat aagcaaaaat catgttcttt
     3121 cctccattat cctttgctga gatttatctt aacaaaaaat tgcattcact gcatgaaatt
     3181 tttttcatgc tttatttccg aagaagcctc ttctggaaca ccatttattc gtaacaaggg
     3241 actgtgacaa gacttttgtc acagtccctt tactgtttta gttaattgat taaaaaggtc
     3301 gtacaagttc cacttgctta attacttggc tgttcttctt cgtcagaaac gatcaagaca
     3361 tcacaaggtg aatgacgtat cacgtagctg gctacacttc ctgtcataaa gcgttcgact
     3421 gcatttagac ctgactgccc aaccatgatt agatcgatct catatttttt tggcaatttg
     3481 actgccatgg attcacgaac agaaccatat gcaataattc cttctatatt tgtgtaatca
     3541 acagatttcc catattcttt acattcttcg atcatttcct tggctgtttc agtttgctga
     3601 tcgactagat tttgatcaat aggtgcatag cccataaagt tataaaattg ctgatcgatt
     3661 acgtttgcta catatacttt cccattatta cggcgagcca cttctaccgc ttttttgaaa
     3721 gcagcaagtg cttgttcact accgtcgata cctactaaaa tattcttata ttcttgattc
     3781 atcttgaccc ctcctaacag atgtcagcct tttgttacac tttctaggaa cgattcgatt
     3841 tcctgttttg ttttacgatc tttattcact agacgaccta attcttttcc gtcttggatc
     3901 acgacaaaac ttgggatacc aaaaatgttc cattcagcgg ctacatctat gtacttgtca
     3961 cggtctactt caataaattg ccaatcagaa aagtcatttt caatttcagg catttgcggt
     4021 ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga aaaatacact ctttccagtt
     4081 tctacgtagc ctgctaattc ttctaaacta tttgggataa tcattctttg tcctctactt
     4141 tctgtttatg tatttttcaa ttaaattata cacctttctt tcgaactaac aaacattgat
     4201 cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt gccagatttt atagtttcac
     4261 tatcgcatta ttttccgtat acgatcgtat ttactgttga tttatccaaa cctttcagtg
     4321 tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc gtttgatgcg
     4381 tatggatata acgtcccact acgccaatca ctgtacttgg gattccttcg ttttgtgtat
     4441 gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg atattgtttg
     4501 tttcagcgat atccagcaag tactcgcgca aacgaggaag catgatcaaa cctggatctt
     4561 ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc gtcactgtat
     4621 catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta ttgacagaag
     4681 ctttagaacc acgcaagcct acttcttcct ggacattcgc gcctgcaatc aatgtatgac
     4741 ctaatgtttc attttgcaac gcttctaaag cttctaatac cattgtacag ccatagcggt
     4801 tgtcccatga tttactgata atattctttc cgttggctgt tttgatcgtt tcagcgtaag
     4861 gaacgattgt gtctcctgga aggacaccgt aactcattgc ctcttctctt gattcaaacc
     4921 cagcatcgaa caaaatatcg gttacttcta ctgatttttg tccgcttgtt cctcgcaata
     4981 aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag gttttaagtg
     5041 taaaacgctg agcagaaaca acgtaaggat tccagccgcc tagaggtacg actttaaaaa
     5101 gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt gctgcgacca
     5161 ttacacgggg tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg cccaaaccat
     5221 ctaactgtaa ctcatctacc agcggttcca tatgactttc catataagcg cgaacatctt
     5281 gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga aatgtttttt
     5341 cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta tattgatctg
     5401 cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta ttttttctgg
     5461 tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataagcac aaggagtaac
     5521 aagtgagaag aaggaggtat gtccacatgt atgaagaaag cgaactaagc tattttaaag
     5581 gcgggttagc agttggaatc ggtctaggat tattgagcgg ggtagcttca acgatttggt
     5641 atcatcgcaa aaaagtattg tcagcagacc aagtacttga aaatatcaag tcatcttttc
     5701 taaaagaagg tgcaatcgaa ggttcttgga tctcttttga aaaggaacct attaggaaat
     5761 tcgccgttcg gtcgagtgct tatcggggag gtctttctcg aatagaagat ggcgaaactg
     5821 tttattatga atttttagca gatgctcata caggaaccgt attagaaatt tcaagaaaaa
     5881 aaggtgcaac agattaatac gtcaaaaaag tgagagaacg aatacgcacc cactattcaa
     5941 aagagaggta tcatgtcctt cttttgtagt gggtggtttt ttagatagag ctttccaaac
     6001 atatattcca gtattttctt tttaaataaa tgatatttag cgggaaaaac tagcatttcc
     6061 tttgattatg aggtattttt aatttagctt ttgttttcta tagttaagga gagtgaaaaa
     6121 tggaacaaca agaaaaaatt gctgttctga ttccttgtta caatgaggaa gcaacaattt
     6181 caacagtcat tgcagacttt aaacgagaat taccagaagc agatatttat gtctatgata
     6241 ataattcgac cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc gtaaaaaaag
     6301 aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc gatgccgact
     6361 attatttaat ggtagatggc gatgatactt atcctgcaga agctgtacat ggtttgctcg
     6421 agaaattacg ttctggtgaa gcagatatgg tcattggtga tcgcctatca aatggaacct
     6481 actttgatga gaataagaga cctttccacg attttggaaa taaccttgtt agaaatacaa
     6541 ttactagaat gtataaaaca aaaattcttg atgtcatgac cggctatcga ggattcaatc
     6601 gtatctttgt caaaagtttt cctattatga gttctggttt tcaaatcgaa acggaattga
     6661 cgatccatgc tttagataaa aaattcaagt tcgttgaaat cccaatcgat tatcgtgatc
     6721 gtcctgaagg cagcgaatca aaattgaata cattctctga cggattcaaa gtgatcatga
     6781 tgattattaa aatgtttaaa gattataagc ctttgctttt ctttgggatt tggaccattt
     6841 tcttcttttt atttggattg gtcacgggga ttcctgtgat tcgcgaatat atgctgacaa
     6901 gctttattac acgcatccct tctgcgattt taagtactgg attgatgact ttagcacttc
     6961 tttcattagt aacagggttg attttggata cagtcgtcac gaacgctaag aaagaatatg
     7021 aattgaatct atatcatgtt tatcatgaat accacagtag aaagtaaaaa aaggatcaag
     7081 actgcaaaaa taatttgcaa ttcttgatcc tctttttaat taattatttc gccaaacgat
     7141 agatggcatc tgcatagatt gctgttgctc tgaacaagtc atcaagactc ataaattcgt
     7201 ttgcttgatg cattgtatca gtatatcctg ggaacatcgc tccataagca acgccacgtt
     7261 tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc gtagcctttt tctccagtgt
     7321 ggtcttcata gacttgcaat aatgtagcca ccaatggatc atccattggt acgtaatgtg
     7381 gttccattac gcgagcacct cgagtaactg ttgcgccttc ttgccccact gtttgttcga
     7441 tgtcactttg caatccgtct actgttacac cttttggaaa acggaaattc atattgatga
     7501 agttcgcttc ctctgtacca ttttcttcaa aagcaaatag tccagcattc attgtcaatt
     7561 tgcccatttt ttcatcttca taagcgactc ctagtttttc tccataaaaa tcttcatgga
     7621 cataagcagc tgctacatgg ataaattgtt tagcagatcc taggaattca tagttatcta
     7681 agaacaatgc caagaaagaa cctgcattga taccagattg tgggcttgca ccatgtgcgc
     7741 ctttaccaac taattcgatt tttacatacg tattgtccgc ttcaatcgtt ccgcttactg
     7801 gatttgcttc gatgaattgg taaaatgctt cttccatcgc gatagctgca tctgcacttg
     7861 gtacttgaag tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt ccggaaacaa
     7921 aagatttcaa gacataatct cctgcattgt cgcctttgaa gcgtaaagct aaagaaacat
     7981 ttcctttttc accattgatg attgggaatt cagcatctgg tgagaaacca aaatctggtt
     8041 cttcttcatg ttcgaaataa taagccatat cgccccagcc gctttcttca tcgctgccga
     8101 ctacgaagcg aacttttttt gatagtttca agtcaagttc tttaatgatc ttcatcgcat
     8161 agtaagctgc catacttgga cctttgtcat cgcttgatcc gcgtgcaaag atctttccat
     8221 ccttgattac tggttcataa ggatctgtgt cccagccatc tcctgcagga acgacatcca
     8281 tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag atcaatatgc cctgcatagt
     8341 tatcgacatt ttttacttta aatccatcac gttcgccata agctaacata tgtttcaatg
     8401 cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac ttttgagtca tcgcgctcgc
     8461 tgttgacacg taaaagattt ttcaagtctt caagcaagtc ttctttacgt gcttctactt
     8521 ctttttgcca atcaattgtc ataaagttaa tcctccactt atttaagtct tcgtgttcat
     8581 gataccacaa attacagaaa aaaatagttc aaaaaatctg acaattcctt ttcttatctg
     8641 tttttctcta aaaaaaatat acgctataat gagtaaaagg aggtttttag atgctgataa
     8701 acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat aaaggaacat
     8761 ttggacgtat cgttttgatt ggcggcaatg aacaatatgg cggagcaatc attatgagtg
     8821 ctttagctgc cgtacacagc ggagcgggtc tcacgactgt agtaactgat gaaaaaaatc
     8881 ggagtgctct tcacagtcat ctgccagaag caatgattgt agtttggacg gatactgacc
     8941 agtttctttc cgtaataaag caagcagatg tactcttgat tggtcccgga ttgggtactt
     9001 cttctaagag tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa gaacaatggc
     9061 tggtgattga tggctctgct ttaagtttac ttgctgctaa tcatcttttg ctaccctatc
     9121 ccgaacaaac tgtacttaca cctcatcaaa tggagtggca acggttgagt tctctaccga
     9181 tacaagaaca acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt gctgtgctcg
     9241 ttttgaaaag tcatgagaca aaaatttatg gtcaccagca cttctacacc aatcctttag
     9301 gttctgctgc tatggcaacg ggcggcatgg gtgacacttt agctgggatg atcacaggat
     9361 ttttagccca gtttcctcaa aatgaccaaa cgattgcagc agctatttat cttcatagtt
     9421 tcattggaga ccagctggca aaagaacaat atattgtttt gccgacacaa atcagcgaag
     9481 cacttccgaa atggatgaat tatttttgta aaaaatcaat ggaaaacgag gcatgagtca
     9541 tggagaaatt tacaggttat gtgcgagaac ctttttatta tgaaacagat caaatgggga
     9601 ttattcatca cagcaattat atccgctggt ttgaagaggc tcgggtagcc ttattggtac
     9661 atctagggtt tccctataaa aaaatcgaag aatctgggat catcatcccc gttctggaag
     9721 taaattgcag ctataaagaa atgatccatt acggagaaaa agtgagcatc catccaacta
     9781 tccaaaagta tacggggaca cggttggatt ttagctacga aattgtggga actgaagaca
     9841 aaaaattaaa aactactggc agtagtaaac actgtttttt actttctgat tcgcaacgat
     9901 tagtgaagct gagcaaagtc aatccggagt tagatcagtt attccaagcg tacgcttctt
     9961 tggatgatgt aaattcttaa taaagaccgt ctaagcattt ttcttgtatt gtgtcgagta
    10021 gttcgtataa tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa agtaatgcac
    10081 ggttacatcg ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa tctgctcatt
    10141 ttcaaagaca aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg tgtcaaaccg
    10201 atccaagagg tacgaccaga ttgattcaat atttttcttt tcgaggctct aataggcatg
    10261 cctatcaggg ctttttttgc agactgtgtt tttatgccca cacgatttgt aagcgattta
    10321 tgttataata gattatatct gcatagaaag gaaggttaaa tggttatcaa taatttgcaa
    10381 cagcaagctt atgaatctat acgtaaacaa atcatttatt ccgagttagc acctggaagt
    10441 aaaatctccg ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc gattcgagaa
    10501 gcactgatcc aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc cggcacttat
    10561 gtttcaaaaa tcgatatccg atctgcttca ttagcccgtt atacgcggga aaagttggaa
    10621 aaaccgatcc tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc taaactagaa
    10681 aaaatactag aacaaaccga tcaggcagta atcgctcaaa ataaaaaagc tttttttgat
    10741 ttggatactg attttcaccg aacttgttat taaattgctg gaaaacgaga aatttgggat
    10801 tggctagaaa gttatagtac gcacttgaac cgtttcagat ggttacgttt gacgatttca
    10861 gaattagact ggggtcgagt attagatgaa catcagacaa tgcttcaatc aatgattgat
    10921 cataactttg atgaagtcgg atttctttgc acgatgcatt tacatatgat tatcgaggag
    10981 caagaatacg tgattcataa ttaccccgat tattttgaag atattcagga cagacccatc
    11041 aaataagagg ttttggaatc agcgttttga cctgagtatt agagaaagaa acggcaaaat
    11101 agttcctcat attttgccgt tttttttgag ctaataccga gggtcagctg attgcacacc
    11161 gtttattgag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa
    11221 aatagcttct tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac
    11281 accgttttcc aagaggtcgt gagcctgccg ttcatctccg agtcacaagg aacaatttca
    11341 aaaaacagct tctcatgttt gttgaatatt ttgacttgtg accgaggagt tgacaggaga
    11401 acatcgttta ttcggaaaaa aaggactgtg acacgacttt tgtcacagtc tctttatttg
    11461 taatctaaat catactccgt acatcttttt tctatttttt ttatttcttc taagatccct
    11521 tggctacgaa tttcattata tggccaaagt aagactgcct ctaatacata aggcgaaaaa
    11581 tcgctttgat tcatctcttt tgaaaaactc atcgctgcta tctcgctcaa tgcacgaatg
    11641 tttgattgat gtgtataaaa aggcaaccgc cacagcacaa ttttttcgct ttgcgtataa
    11701 attcccttaa cagaagcttc gatgtggtga aatagttcat gtccaataat caaagcttgc
    11761 gctgtttctt tagagatcga agctagtcct gaacatttta atttttctaa cggctccgtc
    11821 atcaatcgaa tcccttcatc ttctgcataa gttgcaaaag tgattcgttc gtttatttgc
    11881 tctgattcac aggaaacgat tggaacagcc atttgttcac aaatttctcc tacatcggca
    11941 aaagggtact tttttttgat gctacatgcc gtttcgatac cacaatcgat tgattgttgt
    12001 ataagaattt tctgcttgga caaaggaatc tttttccaaa ataaatctcg ccgaaactgg
    12061 tagaatcccc aaatttcttc ggggatctgt tgtaattcgt ttagtaactc ttctaactta
    12121 tgtttgtttg atatttccgg caacgacgat cacttcttcc tccggttcca tagtagatac
    12181 ttccagatcc atcaaaagct gtagttgttc cagatccgtc gctgtaaaat cagagactct
    12241 tgctcctaag cagagataaa attctggtcg ggcaatcaat tcagtttgat aaactgccat
    12301 atcttcccaa agttgttcga cagctgtcat ataattggct gcagttgttt tcatacatga
    12361 agcatgtcca cgttggacct tgataaatcc tttttgatcg atgatccgaa gacttccagc
    12421 attttttgaa cgattctgtt cgccgcatac ataaaagaaa ggtgtatttt ccaataagca
    12481 aacttcattt ttatttaatc gcatatcctc tgcagcaagc tctagtgctt cttctgttgt
    12541 gatctccttg gttagatcgg ttgctttcac ttccgtagaa ccagtagcga tagccgttac
    12601 ttttgaagtc tgtggatcga tttctacgtg gacttcgatc gattctggtg tagcgcctga
    12661 ttcaatcgct ttattcatcg cttcattttt caacgatcgt atatcttctt tacttggcga
    12721 agggatgata cgttctacaa catctcggac cattgcaagt gctacaccaa tagaagaaat
    12781 cacttcggcg ttttcaggaa tcgaatattt cacacccatt ttattactaa agtaagtaat
    12841 caatgaggca gcgccgcccc ctacacctac aagagaaatc tgatctttct ccaagtgata
    12901 cttgtctgcc aattctaaaa tcaccggttc aatcttagca taggattttt ccatgatctg
    12961 ttctgcgatg tcttcaaccg tcgtatgaca ataatcagct aatgcttgga tggcttttcg
    13021 tgcagatggg acatttccat aagaaaaatg ttcttcttgc actaatccaa ggacattggc
    13081 tgcacatgta tttgtaatag tcacttcttc tccgtcttcc atcactacct tgacgtaatc
    13141 agcagggtct ccttctttag gagagaaaaa ttcaacctta ggacctttga ttttttctgt
    13201 ctccgtaaat actgcataat ctagaccggc aatatgtgca gaacgtggac cgacatcgat
    13261 gattcctgac tgattagccc gtaccatcga accgcctgcc acacctagaa cccgaacatc
    13321 taatgaagag atatatgttg ggtggccacc tacaattgaa taatcgattg ctggacgtcc
    13381 atttttaatg accccgatat tggttgttgt tccgcctact tcaaaataaa caccattaga
    13441 cgctctcaag tacatcaaag agcccatgac tgatgctgct ggaccggaaa gcatggttaa
    13501 aacaggtcgt tttttcattt cattgatttc cattactccg ccgtctccgc gcataatcat
    13561 caaagaaaca ttgacccctg cttctcgtac ggaatcttct gtagaggttg ccgtatctag
    13621 catttttggt aaaatagatg cattgattgc tgctgtccga gttcttcttg tcaatccata
    13681 taattttgtg atgtcagaag ccatcgttgt agggatcgat tttaccgatg ctgcttcata
    13741 aacaactcgt tctggttccc cattatctac tccaaatgcc atagaagaaa ccaagacctc
    13801 cgctctttct ctttctaaag aagaaatcgt ttctgaaaca cggtctacat ttaagtgttt
    13861 gacaggtaag aatgcattga caatctctat ttttttctta ttccctaaat cgatatcgtt
    13921 caatctagtt tgtcgttttg ctaaaaaacc ttctaatcca ccttttgcca tcccgattac
    13981 gccgactttt gctacgtctc cttcaatcag tgcattagtc gcttgtgtgg tagaatgtgc
    14041 aacaaacaca acatcttctg gactgatatt attttccagc aaacaatttt gaaaagactg
    14101 aacgacacct gcggctactc cacggacatc atcgtgggtt gttttcactg aagattttcc
    14161 gatgatttcg tgcgtcgcat tatcgattgc gactgctttc gtatgtgtac cgccaacatc
    14221 aattcccatt cgcacaaaac gtttcatttt cttttcctcc attcatctaa tcgtttttat
    14281 cgacgttttg tttactaggc gttcccgtac aataagaacg tgatcaggca taagatcgct
    14341 ccgatgatcc aaccagttgg gatggatttc ttcatatatt ctcgagaagt tacttttgta
    14401 taacctaatc cccaagcaac ccaagattgt gtaatatcta aatgctgagg agcaatcgtt
    14461 gtgattgcaa ataacgggaa caaaaaggct ggcgacatcg ttggattaac agctaatacg
    14521 acagctaaaa tagctgagcc tgaaccgact agattcattg gaccgcggaa aaatcctagt
    14581 ggtgtcaata tagcaaatac gattgccaag actaatggtg attgcggtat ccaatctcca
    14641 aaaatagctg aaaaataagg agaagcatac gcagctgaat tattgaacat cgctaaggtc
    14701 atcaagaaac caaccattgg cgcaacatct actgcgccat ctgtaaataa tttcgcaaac
    14761 tgacgacaga tttctttata acctccgcgc aattttccag taaacagcaa tgcccagata
    14821 cctgctagaa taaaacctaa gatgattgga agatctaaaa gaacgacccc taaaactggt
    14881 aataatacgg ctaaccatga gatcattggt gcgtcgccac cttcagctgg tacgtttgct
    14941 gccattgcat agcgtttctt tttgttcatt gatatattag cgaccgttag taccacaact
    15001 aagcttacaa tcataccaat cataccaatt tggaaatagt cattataagt atagctttca
    15061 gccgctggat tgaaaccagc ataaatcgtt tgatattgtt tgaaattgac gatattagca
    15121 aagattccag ccataatcga gcccataaat gagaacagtg caaccggtac tgaaatacct
    15181 aatgacatca agattggcaa cacaataact gcaatagaaa tcactggtcc gattcctgtc
    15241 atcgacatga atataacagc ggttacaata catagtaagc tcatcgtgat tcttggcttg
    15301 tcccctccaa gttcaactac ctttcgaatc agggtagctg cgattccgct atctactaga
    15361 acacgcccga aaaaggcacc aaagaagaca tttaccaaga tcgatttggc gtactcagct
    15421 ggccccgtcg tatagatatg cgtcaacaca tcaattactc cttgcccttc catcgccggg
    15481 tttggtgaaa acgtgtttcc gactagcgct aatcccatcc atacagtagc catgatggca
    15541 aagccgacca tcaagttgta gccttttaca cagtaccaga ccatgaaaaa gaatgagata
    15601 actaacaaaa taccaataat aatatcactc atgttattcc tccgtccatt ttgtttttct
    15661 gataagcaaa ctaatatatc agtcaaactc attctaactt atgtagttag cgctttcatt
    15721 aaagggtgac cataaatgtt cagttttcaa ttaaaaaacc ttaattatgc tattttagac
    15781 ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa gaggaaagaa
    15841 cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta gccaaagctt
    15901 cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc aagtcagcta
    15961 acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat tttgagtagc
    16021 tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta cggaagtcgc
    16081 gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact tgttcttttg
    16141 ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt agcactcttt
    16201 tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat tcctcctaat
    16261 aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc tagttgttgg
    16321 cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg acggcgttgt
    16381 ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt caatccgcct
    16441 ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt tggcatgact
    16501 atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag ccagttagtc
    16561 attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg cgtccgtcca
    16621 ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag cgattcaaga
    16681 ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata gaagctttca
    16741 ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg aagtcattca
    16801 agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt tttttacgcg
    16861 cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg attcgcgcaa
    16921 ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt tctgcaatat
    16981 tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg atcaatcgta
    17041 actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc attcacctcc
    17101 aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa cgagttctct
    17161 tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag cccagtgaca
    17221 ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat catagtatag
    17281 caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt ctgaaaatga
    17341 ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa agaaaaacga
    17401 ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt cttacaaagg
    17461 gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac gggggtgacc
    17521 aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa aaacttacga
    17581 tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa aaatgtgatt
    17641 tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat caatgtaaat
    17701 taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact gattgtattg
    17761 cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat cattgtcaat
    17821 tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct gacaatcagt
    17881 tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg actaatttct
    17941 tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac caagagcaag
    18001 cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac gaatccgccg
    18061 ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg cgattcccca
    18121 tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc ctgtcacaat
    18181 agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt cattcaagtt
    18241 gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg aagctaagtt
    18301 cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt ctttttcctt
    18361 ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta ctacattttg
    18421 ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt gcagcagatt
    18481 cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac tattcacttc
    18541 ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca ataaactgta
    18601 tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca cgtactcggt
    18661 attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga taggactagt
    18721 ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca gtaacgcttc
    18781 atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat aagtcagcat
    18841 ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat cgttttgctg
    18901 tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca tactctatcc
    18961 tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt taccctttat
    19021 ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta atttttcttc
    19081 gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat agcggtctcc
    19141 atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt aacgtctgtt
    19201 caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat acgaacgacc
    19261 acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa ctaataaatc
    19321 cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca gcagttcatc
    19381 atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat ggaaaagcag
    19441 tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg cttgtttgat
    19501 gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact gttcagggtt
    19561 tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg cctgactatc
    19621 aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg tacctgctaa
    19681 aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt ttaactccaa
    19741 cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc tagtcaaaga
    19801 agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa acgtattctt
    19861 agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg agacccttag
    19921 tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt tttgcttaaa
    19981 aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg agaatcaagc
    20041 ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc gattgccttt
    20101 cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat ggacgatcaa
    20161 ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt aaaaaatatc
    20221 aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt gcaactgacc
    20281 attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt cgccacagca
    20341 gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat actattagaa
    20401 aatgtccagc tttcggaaaa aatcgcccat ggtaatccta gtggaatcga tgcagccgct
    20461 actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta cttttctttg
    20521 aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac aagagaagcc
    20581 gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca aatgatccaa
    20641 aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc accagaaacg
    20701 ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat cagcaatgat
    20761 tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc caaattaact
    20821 ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa agcccaagaa
    20881 atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg attaggagta
    20941 catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa tcaaatattg
    21001 gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt taacattgga
    21061 tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag atgaatttta
    21121 tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt ttcttgacct
    21181 ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga atttcgttcc
    21241 taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag gcgcttgcaa
    21301 tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc gacgcgggtc
    21361 tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag gccatgacga
    21421 cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt tagcaatggt
    21481 tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga tgcgtcggac
    21541 agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg atctatatca
    21601 attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga tggaaagaaa
    21661 cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt actggtctcc
    21721 tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca ttccatgcta
    21781 ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc atttatctga
    21841 ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg cacatgctgg
    21901 tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat ctgcaccagg
    21961 caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag cagttatcgc
    22021 tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg gaagtatcca
    22081 atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag aattagtttt
    22141 agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga ctgaaaagta
    22201 tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa gtgagttaga
    22261 cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg ttgcaacggt
    22321 caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt tcaagattgc
    22381 agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg ctgctagctg
    22441 ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag aacaagaaac
    22501 tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca ttgagccatt
    22561 gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg cctctacttc
    22621 tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt atcagctttt
    22681 cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag aaaataatgt
    22741 aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt tatctgcaat
    22801 cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag ctgaacagta
    22861 tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg taattgttga
    22921 ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa tcactccact
    22981 gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa agacgaacat
    23041 gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt cgtacggatc
    23101 atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac acaggcagtt
    23161 gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag cgaaaaaaca
    23221 aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat gattgccact
    23281 ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac gattatgcga
    23341 caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc tgtcgaaaga
    23401 gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt gaatgcacct
    23461 caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt acttattcaa
    23521 gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt tggcatgaca
    23581 agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat cagcggtcga
    23641 agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga actgagttat
    23701 ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga agcagacact
    23761 tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt taaagccctt
    23821 tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct gatgaatcac
    23881 ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg tctgctttat
    23941 actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat cttttccgga
    24001 ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg aagacgcaca
    24061 gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtacaaa
    24121 cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
    24181 aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca gcctcttttt
    24241 tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc tgatttcaca
    24301 acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat ataggcaagt
    24361 atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct agaagccaag
    24421 aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg cgtacgtcca
    24481 acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg taatggagca
    24541 gtttgcggtg tagcaatata acctggtcca attccattac attggatatt gtattgaccg
    24601 tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc agcataagca
    24661 ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat tttaccgccg
    24721 cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc atttaaatcc
    24781 acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt ccgtttgatg
    24841 atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat ctgttgaacc
    24901 atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc atcgatgcct
    24961 gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt attaaatacg
    25021 atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc atagacagca
    25081 ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa ttccatcatt
    25141 ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt ctgtataggt
    25201 gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta cacctgaatg
    25261 gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat gttttgtttc
    25321 atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat caaagtagac
    25381 ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag cgcttcctgg
    25441 ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg gatggatgta
    25501 ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg gttttacttg
    25561 gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac taatatagaa
    25621 tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt cttttccgat
    25681 atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga tgaatcctgg
    25741 tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa cacctagttc
    25801 tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa agatcattcg
    25861 atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt ctactaaaaa
    25921 ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt gtgtataacg
    25981 tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc cagagccgca
    26041 tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat ggatcgtatg
    26101 cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca tattggataa
    26161 aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat ccacgattgg
    26221 ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg tccccttcaa
    26281 ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct gaatattcgg
    26341 gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg cttctggcgc
    26401 tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga tatctactaa
    26461 aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt ttccacgata
    26521 gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca cctgtttcgt
    26581 cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa atatatctat
    26641 tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa aacttgatcc
    26701 ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca tataataagt
    26761 acctagtctt gatccaccag ataatacctg tttcgtatct actccataac attgcaagtg
    26821 ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa tcgcttcatg
    26881 tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat gggcagcgaa
    26941 tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca tgatttctcc
    27001 gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt ttgctgcata
    27061 ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg ctaaaaggtt
    27121 cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat tgtccagact
    27181 cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg cagaaatgat
    27241 acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc ttttcagcgc
    27301 tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat acagattgca
    27361 catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc cagctaagat
    27421 agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta tttctggttg
    27481 gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga ctgtaaaagt
    27541 caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca aagcttcttc
    27601 tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag ataaaatatc
    27661 aatttttttc atcttctcca cctatttcct atttagaaat tttattttat aacttggaaa
    27721 tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc attatttttg
    27781 aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt gttcttttgt
    27841 ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga tttgttcgtt
    27901 tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat cgtcaaatgc
    27961 gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat tggtgatcgt
    28021 atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact gctcttcact
    28081 gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg tgattccgat
    28141 ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt acaagatctc
    28201 attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca gctgttctaa
    28261 aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg cgtagcgaat
    28321 aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc cgatcatcaa
    28381 caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct ttgcgatcgt
    28441 ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg ctttgatcaa
    28501 tacagttcca taaggctttt tattttccat ataatattct cctatttctt tataagaaaa
    28561 attatagcgg aaataccatc aatttaaaag agatatccag atataaacta ccatttctct
    28621 ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt gatataaagc
    28681 aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat ctcgcaaagc
    28741 atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa tggtttctgg
    28801 gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc ttacatcttc
    28861 taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac ggtacttttc
    28921 tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac acgctacgcc
    28981 atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc
    29041 gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt ctcgcccatt
    29101 ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg cttttaaatg
    29161 cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag catgcgtcat
    29221 agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca tatcgctgtc
    29281 gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc ggtgatttac
    29341 ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag tcgttggtat
    29401 agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac taatgacgtc
    29461 tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga ttggcgaacc
    29521 tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca ctccatcttt
    29581 tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag cgattgctgc
    29641 tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt
    29701 aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg actcaatcgc
    29761 tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt tct
//
LOCUS       DAJSVD010000026        28644 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSVD010000026 DAJSVD010000000
VERSION     DAJSVD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.026"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(181..1125)
                     /locus_tag="ON498_001014"
     CDS             complement(181..1125)
                     /locus_tag="ON498_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939306.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            1346..2560
                     /locus_tag="ON498_001015"
     CDS             1346..2560
                     /locus_tag="ON498_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939307.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            2582..4597
                     /locus_tag="ON498_001016"
     CDS             2582..4597
                     /locus_tag="ON498_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR3939308.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(4680..6053)
                     /locus_tag="ON498_001017"
     CDS             complement(4680..6053)
                     /locus_tag="ON498_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3939309.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(6079..6363)
                     /locus_tag="ON498_001018"
     CDS             complement(6079..6363)
                     /locus_tag="ON498_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3939310.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(6365..8380)
                     /locus_tag="ON498_001019"
     CDS             complement(6365..8380)
                     /locus_tag="ON498_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3939311.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(8559..8864)
                     /locus_tag="ON498_001020"
     CDS             complement(8559..8864)
                     /locus_tag="ON498_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR3939312.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(8953..9984)
                     /locus_tag="ON498_001021"
     CDS             complement(8953..9984)
                     /locus_tag="ON498_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3939313.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10191..11066
                     /locus_tag="ON498_001022"
     CDS             10191..11066
                     /locus_tag="ON498_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3939314.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(11218..12060)
                     /locus_tag="ON498_001023"
     CDS             complement(11218..12060)
                     /locus_tag="ON498_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3939315.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(12152..14029)
                     /locus_tag="ON498_001024"
     CDS             complement(12152..14029)
                     /locus_tag="ON498_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3939316.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(14026..15447)
                     /locus_tag="ON498_001025"
     CDS             complement(14026..15447)
                     /locus_tag="ON498_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3939317.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(15450..18839)
                     /locus_tag="ON498_001026"
     CDS             complement(15450..18839)
                     /locus_tag="ON498_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3939318.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            19096..20487
                     /locus_tag="ON498_001027"
     CDS             19096..20487
                     /locus_tag="ON498_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939319.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(20560..21207)
                     /locus_tag="ON498_001028"
     CDS             complement(20560..21207)
                     /locus_tag="ON498_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3939320.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(21215..21901)
                     /locus_tag="ON498_001029"
     CDS             complement(21215..21901)
                     /locus_tag="ON498_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939321.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(21907..22980)
                     /locus_tag="ON498_001030"
     CDS             complement(21907..22980)
                     /locus_tag="ON498_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939322.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            23202..23876
                     /locus_tag="ON498_001031"
     CDS             23202..23876
                     /locus_tag="ON498_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939323.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            23881..25221
                     /locus_tag="ON498_001032"
     CDS             23881..25221
                     /locus_tag="ON498_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3939324.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(25370..26356)
                     /locus_tag="ON498_001033"
     CDS             complement(25370..26356)
                     /locus_tag="ON498_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR3939325.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            27080..28111
                     /locus_tag="ON498_001034"
     CDS             27080..28111
                     /locus_tag="ON498_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3939326.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            28220..>28644
                     /locus_tag="ON498_001035"
     CDS             28220..>28644
                     /locus_tag="ON498_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3939327.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8492 a         5354 c         5190 g         9608 t
ORIGIN      
        1 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
       61 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
      121 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
      181 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
      241 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
      301 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
      361 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
      421 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
      481 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
      541 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
      601 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
      661 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
      721 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
      781 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc
      841 ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat
      901 ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg
      961 aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc
     1021 tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat
     1081 tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt
     1141 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     1201 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     1261 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     1321 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     1381 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     1441 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     1501 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     1561 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     1621 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     1681 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     1741 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     1801 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     1861 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     1921 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     1981 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     2041 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     2101 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     2161 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     2221 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     2281 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     2341 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     2401 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     2461 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     2521 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     2581 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     2641 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     2701 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     2761 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     2821 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     2881 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     2941 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     3001 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     3061 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     3121 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     3181 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     3241 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     3301 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     3361 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     3421 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     3481 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     3541 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     3601 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     3661 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     3721 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     3781 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     3841 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     3901 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     3961 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     4021 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     4081 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     4141 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     4201 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     4261 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     4321 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     4381 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     4441 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     4501 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     4561 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     4621 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     4681 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     4741 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     4801 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     4861 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     4921 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     4981 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     5041 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     5101 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     5161 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     5221 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     5281 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     5341 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     5401 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     5461 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     5521 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     5581 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     5641 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     5701 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     5761 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     5821 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     5881 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     5941 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     6001 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     6061 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     6121 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     6181 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     6241 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     6301 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     6361 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     6421 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     6481 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     6541 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     6601 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     6661 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     6721 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     6781 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     6841 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     6901 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     6961 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     7021 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     7081 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     7141 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     7201 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     7261 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     7321 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     7381 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     7441 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     7501 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     7561 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     7621 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     7681 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     7741 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     7801 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     7861 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     7921 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     7981 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     8041 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     8101 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     8161 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     8221 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     8281 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     8341 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     8401 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     8461 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     8521 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     8581 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     8641 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     8701 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     8761 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
     8821 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
     8881 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
     8941 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
     9001 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
     9061 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
     9121 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
     9181 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
     9241 ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg gacgttcttt
     9301 gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa aatccagatc
     9361 gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc tgatatcatc
     9421 tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc catataaata
     9481 gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga aataatcata
     9541 atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt tttggactgt
     9601 caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac ctttttcttt
     9661 tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt tctctactag
     9721 tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt gactaggtgt
     9781 agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct gatcagtata
     9841 ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt ttttgatatt
     9901 gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac tctttccatt
     9961 gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt ttcagtatat
    10021 cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt cagaaaaaca
    10081 cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa tcaaaaagat
    10141 agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc atgcttattg
    10201 aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt gtcgattaca
    10261 tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca aaagctacgt
    10321 atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc ggatttaaag
    10381 aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc aatcaaacga
    10441 ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca aagaaaatgg
    10501 cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat gaagatttag
    10561 atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca aaaggtgatt
    10621 ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag tttttgatca
    10681 tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc gaggattgcg
    10741 ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg acctatttga
    10801 agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa atggccaaac
    10861 aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa gtagcgactt
    10921 tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg atatattctc
    10981 aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg caaaaaggcc
    11041 ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa gaaagatagt
    11101 gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct ttttcaaacc
    11161 tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga ggaatagcta
    11221 acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga ggaaaatcag
    11281 tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata ctttctgtac
    11341 ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa gtaagcgatg
    11401 actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc ctttttcgat
    11461 tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt gataagcgag
    11521 tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt
    11581 gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg tattttctcc
    11641 ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca gcacagtatt
    11701 cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa taccgatcgt
    11761 atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag taaagggatc
    11821 tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca tgttttcttg
    11881 gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat gagagatgat
    11941 ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta gtatccctat
    12001 tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct ttggtttcat
    12061 ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc
    12121 gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa
    12181 agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca tagattccct
    12241 taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca ggagaaatca
    12301 actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg tttgttaatg
    12361 gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat tttagacctg
    12421 tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct ttcgccgata
    12481 cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg tcactatctt
    12541 gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta
    12601 ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt tgatcatttg
    12661 tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct gctttttcgt
    12721 tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa ggaatataat
    12781 tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa tttactgggt
    12841 cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa tcattatcaa
    12901 atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc
    12961 cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga attttttcac
    13021 cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta gacgtatcat
    13081 ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg
    13141 tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa ttatattttc
    13201 caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg gcaaagtcat
    13261 tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg
    13321 tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt ggtgttcctt
    13381 cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca gtaccgattt
    13441 ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga tggatagtat
    13501 ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc tcatatacag
    13561 ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct tctttgatcg
    13621 tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca tttccatcag
    13681 catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact gcttgttttg
    13741 ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa
    13801 ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt tctttcccgc
    13861 tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg atgacttgag
    13921 ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt aatggtaaaa
    13981 taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt ttttcactcc
    14041 tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac caacaacgcc
    14101 taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat tttctttttt
    14161 attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt
    14221 aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct cttttaatcg
    14281 ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg aaatcaggtg
    14341 aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct ggaggtaatt
    14401 tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg gtttatttgt
    14461 ctcgattttg acaaattgct tactccctgt atatacagag actttctttg tactgacacc
    14521 aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct
    14581 tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca acgaagggat
    14641 cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt
    14701 ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt catccgcact
    14761 atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg tatgcaagga
    14821 ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata ccttctcaaa
    14881 aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag ttgaaagttt
    14941 ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct tttctttgac
    15001 gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt cttgatcttt
    15061 agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag ttgttgcttc
    15121 tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac
    15181 cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa cttggaatgt
    15241 gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt catttggatg
    15301 atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct gaaccatttc
    15361 tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca agaagagaca
    15421 catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa
    15481 agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc taaacggcca
    15541 attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa agaaatctgg
    15601 ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac ttgaattgac
    15661 ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc ttctggtgta
    15721 aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa ggtatctgtt
    15781 tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct gaattttgct
    15841 ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt
    15901 cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt tcccgtgtaa
    15961 atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt atcatatccg
    16021 gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc aaaactggca
    16081 agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt tgcttctttt
    16141 ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc ttgcatcgca
    16201 tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa ctggatcgta
    16261 tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga agcgatcttg
    16321 atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt ttctgtcagc
    16381 gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt tccgtcgcca
    16441 tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc gccaataaga
    16501 tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt gaattgttta
    16561 gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac aggtaattct
    16621 ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag tgataatatc
    16681 tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg ttcccacgta
    16741 tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca agctgcagca
    16801 tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc agctagattg
    16861 ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc tttagcagat
    16921 ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt tgtcctgccg
    16981 ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat tctcacttgg
    17041 taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt gatggtattt
    17101 ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact cttgactgaa
    17161 agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg tccattgctt
    17221 acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc tgttgcatga
    17281 tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg tcgcatacga
    17341 gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg gatgccaaga
    17401 ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt tgcgataaac
    17461 gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat tcttgagatt
    17521 agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta ataattgctg
    17581 cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc atccgtcagc
    17641 aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc atctcttagt
    17701 gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt tacttgattt
    17761 ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata tccttcgctt
    17821 gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc taaagtatcg
    17881 acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt catacttcct
    17941 gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac atttccacga
    18001 gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata ctttcgtatc
    18061 gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt ccgatcatta
    18121 ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt attgccttga
    18181 tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata agtgccctga
    18241 tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac agctggcaag
    18301 gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga tgattcttct
    18361 tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc tgtctcatca
    18421 ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg gtaaagtgct
    18481 ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat ttccaacgta
    18541 attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc cacatcactt
    18601 aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt gttattcgca
    18661 tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc agctgaaatt
    18721 gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg cacaaagttt
    18781 gataaactca atattatcag catgaaaata actttcagtt tcttccctgt ggtcgtcatt
    18841 tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa
    18901 caggcgttac ataattttat ttattttaat atgataatta ttatacgaag ggcgctaaag
    18961 ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc cttcgattaa
    19021 tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag gttgttggaa
    19081 ggaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga caagtggatg
    19141 actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt aaggtatatc
    19201 gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca aaaattgggg
    19261 tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa
    19321 agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa aacgattgag
    19381 tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt gaaacaatgc
    19441 aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt tcagttaact
    19501 agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt aagttactac
    19561 ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag
    19621 aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc tttctttatt
    19681 atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga atggaaagct
    19741 ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat cttgaaaaat
    19801 ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt tgtcaatacg
    19861 attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat
    19921 atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt
    19981 caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg
    20041 acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct
    20101 ggatttcgtg cagaacttca attttattcc actctactga atcagaatct gatttattat
    20161 ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct
    20221 gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa
    20281 tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa
    20341 gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt
    20401 ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa
    20461 gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct
    20521 gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
    20581 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
    20641 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
    20701 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
    20761 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
    20821 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
    20881 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
    20941 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
    21001 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
    21061 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
    21121 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
    21181 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
    21241 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
    21301 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
    21361 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
    21421 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
    21481 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
    21541 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
    21601 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
    21661 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
    21721 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
    21781 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
    21841 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
    21901 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
    21961 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
    22021 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
    22081 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
    22141 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
    22201 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
    22261 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
    22321 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
    22381 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
    22441 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
    22501 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
    22561 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
    22621 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
    22681 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
    22741 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
    22801 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
    22861 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
    22921 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
    22981 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
    23041 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
    23101 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
    23161 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
    23221 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
    23281 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
    23341 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
    23401 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
    23461 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
    23521 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
    23581 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
    23641 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
    23701 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
    23761 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
    23821 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
    23881 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
    23941 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
    24001 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
    24061 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
    24121 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
    24181 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
    24241 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
    24301 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
    24361 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
    24421 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
    24481 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
    24541 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
    24601 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
    24661 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
    24721 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
    24781 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
    24841 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
    24901 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
    24961 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
    25021 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
    25081 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
    25141 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
    25201 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
    25261 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
    25321 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
    25381 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
    25441 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
    25501 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
    25561 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
    25621 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
    25681 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
    25741 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
    25801 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
    25861 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
    25921 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
    25981 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
    26041 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
    26101 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
    26161 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
    26221 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
    26281 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
    26341 aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta
    26401 cttataaatt tattctatag gagacgattg ttttctacaa atatttagat tggaatttga
    26461 ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat attctaataa
    26521 aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc cactcgttgt
    26581 gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa aggattaatt
    26641 ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga agtaccaact
    26701 atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc attattgaat
    26761 aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag
    26821 agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta accacatagc
    26881 taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata aaataactaa
    26941 aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt tgcttcctaa
    27001 gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag aaaatgaaca
    27061 ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag aaaagttaaa
    27121 gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa atcgttcttc
    27181 cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta ctgattattt
    27241 gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag aaaaacaaga
    27301 ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa ctacagcgaa
    27361 ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga agcaatattc
    27421 attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag agatttctct
    27481 tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg atgcagaact
    27541 tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa gcagagaagc
    27601 tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc ttctgtattg
    27661 cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt tgaccaagca
    27721 gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac caaaagaagc
    27781 gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg aaaattcttt
    27841 tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc aaattttgag
    27901 caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat atcgttatta
    27961 tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta atcgatacct
    28021 ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt atcacgaatt
    28081 agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt tttcacaata
    28141 aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat attacttaac
    28201 aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga acttatttac
    28261 gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg atgagccact
    28321 ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt
    28381 gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag ttattattta
    28441 taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc ttggtgcagt
    28501 gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta tcgatcgttt
    28561 agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa
    28621 tggaacaaaa tcaaaacaga ttcc
//
LOCUS       DAJSVD010000027        27649 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSVD010000027 DAJSVD010000000
VERSION     DAJSVD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.027"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(239..2131)
                     /locus_tag="ON498_001036"
     CDS             complement(239..2131)
                     /locus_tag="ON498_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939328.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2128..3855)
                     /locus_tag="ON498_001037"
     CDS             complement(2128..3855)
                     /locus_tag="ON498_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939329.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3871..4521)
                     /locus_tag="ON498_001038"
     CDS             complement(3871..4521)
                     /locus_tag="ON498_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3939330.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4725..5021
                     /locus_tag="ON498_001039"
     CDS             4725..5021
                     /locus_tag="ON498_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3939331.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5193..5813
                     /locus_tag="ON498_001040"
     CDS             5193..5813
                     /locus_tag="ON498_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3939332.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5905..7071)
                     /locus_tag="ON498_001041"
     CDS             complement(5905..7071)
                     /locus_tag="ON498_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3939333.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7083..7436)
                     /locus_tag="ON498_001042"
     CDS             complement(7083..7436)
                     /locus_tag="ON498_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3939334.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7564..8586
                     /locus_tag="ON498_001043"
     CDS             7564..8586
                     /locus_tag="ON498_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR3939335.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8605..8766
                     /locus_tag="ON498_001044"
     CDS             8605..8766
                     /locus_tag="ON498_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939336.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9025..9459)
                     /locus_tag="ON498_001045"
     CDS             complement(9025..9459)
                     /locus_tag="ON498_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939337.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9569..11428)
                     /locus_tag="ON498_001046"
     CDS             complement(9569..11428)
                     /locus_tag="ON498_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3939338.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11546..12706)
                     /locus_tag="ON498_001047"
     CDS             complement(11546..12706)
                     /locus_tag="ON498_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR3939339.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13013..14437)
                     /gene="araA"
                     /locus_tag="ON498_001048"
     CDS             complement(13013..14437)
                     /gene="araA"
                     /locus_tag="ON498_001048"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR3939340.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14453..15151)
                     /locus_tag="ON498_001049"
     CDS             complement(14453..15151)
                     /locus_tag="ON498_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3939341.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15108..16769)
                     /locus_tag="ON498_001050"
     CDS             complement(15108..16769)
                     /locus_tag="ON498_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR3939342.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVSGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16987..18387
                     /locus_tag="ON498_001051"
     CDS             16987..18387
                     /locus_tag="ON498_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR3939343.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18639..19718)
                     /locus_tag="ON498_001052"
     CDS             complement(18639..19718)
                     /locus_tag="ON498_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3939344.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19804..20763)
                     /locus_tag="ON498_001053"
     CDS             complement(19804..20763)
                     /locus_tag="ON498_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3939345.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20775..22286)
                     /locus_tag="ON498_001054"
     CDS             complement(20775..22286)
                     /locus_tag="ON498_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3939346.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22308..22871)
                     /locus_tag="ON498_001055"
     CDS             complement(22308..22871)
                     /locus_tag="ON498_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3939347.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22877..23740)
                     /locus_tag="ON498_001056"
     CDS             complement(22877..23740)
                     /locus_tag="ON498_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939348.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23754..24650)
                     /locus_tag="ON498_001057"
     CDS             complement(23754..24650)
                     /locus_tag="ON498_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3939349.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24825..26114)
                     /locus_tag="ON498_001058"
     CDS             complement(24825..26114)
                     /locus_tag="ON498_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3939350.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26086..27603)
                     /locus_tag="ON498_001059"
     CDS             complement(26086..27603)
                     /locus_tag="ON498_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3939351.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8070 a         5836 c         4785 g         8958 t
ORIGIN      
        1 tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt
       61 aaaatcagcg gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt
      121 ttgccgtttt ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa
      181 gggactgtga cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct
      241 atgaagcttc ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt
      301 ccatcaatgt atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt
      361 tgtctgcatc gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca
      421 ataggcggtt catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg
      481 cttcatcaag gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct
      541 gacgctgccc ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg
      601 gcaatttacg aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt
      661 ctgttgcatc gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag
      721 tatcttgcag taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat
      781 ctacaccatc gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga
      841 tcagtgtcgt cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga
      901 catccaagtt aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt
      961 caaaacgaac tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca
     1021 tctcttcttc atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag
     1081 tgttcatcaa acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga
     1141 acgcttgaac atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag
     1201 cgacgaacac atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa
     1261 taaattgagc tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct
     1321 ggtctttttc agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta
     1381 cttggttatt caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg
     1441 gcgctacgat catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca
     1501 attgccagct gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta
     1561 caaattgagt caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc
     1621 gagacatgat atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt
     1681 tggcttccaa atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa
     1741 attgctgtaa aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga
     1801 tctgcgcgat tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct
     1861 gttgagcgcc agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg
     1921 gtgtttggat ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa
     1981 ccatacgttt agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct
     2041 tttcaccttt tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc
     2101 catgagaggg acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg
     2161 aacgatttct tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac
     2221 cagttttcct tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg
     2281 agcaactaaa acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc
     2341 atccgttttg aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt
     2401 gactaatgcc cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc
     2461 ttgttccaca tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc
     2521 gatttctaat gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt
     2581 gtctcggatc gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg
     2641 caaattatat tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc
     2701 gatgtcatat aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc
     2761 tatgattgcg acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt
     2821 ttctgctcca tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc
     2881 aaaagaaact gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc
     2941 attaatacgg tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat
     3001 cataaagctg atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc
     3061 caaagtcata tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct
     3121 catgatcaat gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt
     3181 atttgtataa tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa
     3241 tgctcgaatc actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt
     3301 tttcttttgc atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat
     3361 aatcattact gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc
     3421 aagtatgctc gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg
     3481 tgtaaccatt tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc
     3541 atatttatca aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa
     3601 ttgcttaccc agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa
     3661 gatactgatc aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc
     3721 tccttgcgct acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc
     3781 cagtacttgg atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt
     3841 tacgattttt agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga
     3901 aagtttcaaa ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa
     3961 taagcgatac gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa
     4021 ttccaattca tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc
     4081 ttttgcatct tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg
     4141 aggagcgact tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga
     4201 agcattctcg ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt
     4261 gaatagatca ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct
     4321 cattgtctca aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact
     4381 gcctctaggg atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac
     4441 tctagaaaac tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg
     4501 gaaaaatgtc tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat
     4561 gacgctgtgt cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc
     4621 attatcatcc gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga
     4681 gttgcccatt aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta
     4741 gtgtccaaga cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa
     4801 ataccttaga ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct
     4861 tgccaagaca attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag
     4921 gcaggcgatc agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata
     4981 tcattggtgg gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca
     5041 aaagaggctg tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt
     5101 atttattctt caaccttatc aatatgactt gatagccttt tctttttctc atagttataa
     5161 caatcatagc aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc
     5221 atcccctaac tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt
     5281 ataaagaaaa tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc
     5341 cggaaatcga tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca
     5401 cttccttatc agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt
     5461 tccttgctgc tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa
     5521 aattaaaagc ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa
     5581 acgggccaga aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg
     5641 tatacaacgg acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca
     5701 tcgatcaagt agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg
     5761 agttgatgca aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga
     5821 gataaatgca tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga
     5881 tattttccca tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata
     5941 aagagaagag attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc
     6001 ataaacagga atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag
     6061 aactaataaa atcgttactg gatggattgc taactgattc cctagtacta atggagagat
     6121 caagcgtcct tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa
     6181 tggacctgct acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta
     6241 agggatcagg ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat
     6301 gaccgcaaaa ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct
     6361 tatataagaa gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat
     6421 gcgtgtcggt aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata
     6481 aaataagata aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa
     6541 acttccaaga ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt
     6601 catgaactta tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg
     6661 caaaatctcg gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa
     6721 gctcactgtt tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc
     6781 aacaaccaaa acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa
     6841 gtaatcaact accggattca tcaagtaata caagattcct gctaaaatga taggcagacc
     6901 tacgattgct aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa
     6961 gacgatcagc aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa
     7021 ccagcgccaa aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc
     7081 tgttagttat aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga
     7141 tagattccat ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga
     7201 gctactgtct ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga
     7261 tcatcaaaag caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct
     7321 tgaatcaccg aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg
     7381 ggttcttcct tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca
     7441 ctctctcctt taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg
     7501 aataattatg tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct
     7561 catatgatcg aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat
     7621 acgatcgacc ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa
     7681 aatagcccta cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg
     7741 gatggattag gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca
     7801 gagtctggcg ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg
     7861 gcgaactatc ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa
     7921 gcagcagatt caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg
     7981 catgtccact atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca
     8041 gatcgtgtgt atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc
     8101 actgcagagc caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct
     8161 tatttatttg gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt
     8221 gcttttgaag aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga
     8281 ggggcagcta tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat
     8341 aactcgattg ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc
     8401 atctcaacag aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc
     8461 gtgtgtgcca atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga
     8521 ttactggaat tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat
     8581 cgttagtaag aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa
     8641 gaactaaata ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa
     8701 tatcagaaac tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa
     8761 aactaatcag ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc
     8821 ctgatgttct tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag
     8881 tatttactcg attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat
     8941 aaaggatttt gatcactcta tcataaaggt aatattacat ttatattaga taactctagt
     9001 ttttattgtt atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat
     9061 ggcagctgtc aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc
     9121 ttcatctacc cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata
     9181 tgaaagataa gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa
     9241 agtactgact ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat
     9301 gatctcttca agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct
     9361 gatcacttca tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg
     9421 atcgatggac tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc
     9481 attgttgtta ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat
     9541 ttaatggttt ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac
     9601 tatattgttt ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt
     9661 gttgcgagcg attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc
     9721 gttcaaacgc cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg
     9781 tctgattcgt ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt
     9841 gagtcgtttg cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca
     9901 atgtttgttt gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc
     9961 cccaaaaaac aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca
    10021 gtgccgtgct gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc
    10081 catctgaagt ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg
    10141 tctgcatcgt gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta
    10201 cttctttgta agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg
    10261 tcatcgtacg ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt
    10321 tatacatcga agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg
    10381 ctgaaacgat agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga
    10441 gttgcgcatt aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg
    10501 ttccaccgcc atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat
    10561 aataatctgc caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga
    10621 aactttcact catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt
    10681 catcggctag cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt
    10741 caggttcatc cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt
    10801 tatagtagtt cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc
    10861 gcaacaaatt atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa
    10921 gcagtgtcgt taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt
    10981 accagcaaaa caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag
    11041 tgccggtcat atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa
    11101 tcggttcttg ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta
    11161 cgacatacgt aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa
    11221 taatcagcaa gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt
    11281 tcaacgcaag gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa
    11341 caacaagaaa aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg
    11401 tcgaaagtct gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga
    11461 atgttgcttc tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt
    11521 aatgaaatcc aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct
    11581 ttcgtactcc agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag
    11641 tactgtctat gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg
    11701 cgatcgctag ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct
    11761 tttgataaaa tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat
    11821 tgaccgcttc tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac
    11881 gtccgctgtc tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac
    11941 ggtcattttc taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt
    12001 tttccaatgc ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa
    12061 gtacagcatc ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt
    12121 ctgtcaacgg cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg
    12181 cttgccgaac ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta
    12241 agctatagca acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg
    12301 ttttgccttg caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa
    12361 tattaggacc tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata
    12421 agagcaaaca gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca
    12481 tgacgatcgt cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag
    12541 ctcttgaaat tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa
    12601 taactattcc ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt
    12661 ctatctcttg ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt
    12721 actaacaagt atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga
    12781 agcaaaaaga gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt
    12841 actgccataa gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg
    12901 tgttccgtta gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt
    12961 cgtcaatctc ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa
    13021 taaccactgt ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat
    13081 ttagcgagac aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat
    13141 ggaaattcgg tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg
    13201 ttggttcaaa ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca
    13261 tcgatacgac taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc
    13321 tatctcccat tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat
    13381 ctacttccat catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat
    13441 aatcttccat gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg
    13501 ctgcagtttt ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta
    13561 cagctagtcc aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt
    13621 tatagcctcc ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta
    13681 catgttcctc ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt
    13741 actcttcaaa taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat
    13801 ctccgatacc aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc
    13861 cttctgtgac acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac
    13921 tttcattata ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc
    13981 gtttccagta gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat
    14041 attcgcggtc accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc
    14101 aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg
    14161 tcccgcggat ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta
    14221 cttcttctcg ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta
    14281 attcttgaag gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt
    14341 cttgtgcatg tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta
    14401 caacaaacca aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt
    14461 tttgtccgta atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt
    14521 gaggcagctc ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt
    14581 catctagtac gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac
    14641 cgtgtccgtg taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg
    14701 tttctatgat aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca
    14761 aaaatcgagc acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag
    14821 gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc
    14881 ctccgatttg aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat
    14941 tgagtttacc ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca
    15001 tctcttcata actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag
    15061 cgctcacatt tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt
    15121 ggtatacttc ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga
    15181 tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag
    15241 cctgctattt cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct
    15301 tctgccaaga agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac
    15361 gcgattcccc acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata
    15421 gcagatgcta gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact
    15481 aaacgatcaa tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca
    15541 aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta
    15601 cgtacaaata atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca
    15661 taagaaagta atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg
    15721 aataatttat ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg
    15781 acccaagcat tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct
    15841 gaaggtgtag tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg
    15901 atcatagcga aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc
    15961 gtagcgacca taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc
    16021 aaatttcccg tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct
    16081 cctgatacta gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg
    16141 attaactgac caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata
    16201 ggaaacatgc ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg
    16261 acataacctg caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa
    16321 atagattgat acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc
    16381 gttaatattc gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt
    16441 aactgttcct tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac
    16501 ccgattgtcg taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa
    16561 ctgacgcgca gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt
    16621 ttgttttccc actcataact accagcggca ataggagtaa aagatgaatc aatcaataca
    16681 gctttgatcc tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct
    16741 aacatacttt gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc
    16801 tgatcaaacc gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat
    16861 gtcaagcggt tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta
    16921 taaaatgaaa ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga
    16981 atacttatga aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt
    17041 ggcggcattc tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag
    17101 catgattggg gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg
    17161 ttaggtgcga tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga
    17221 aaaatgattc ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc
    17281 cctcacaatg gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc
    17341 gctgcatctg ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga
    17401 agattgtcag ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca
    17461 gattatttgc taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct
    17521 gctgtccccg cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt
    17581 cttatgcaat ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca
    17641 aaagaagctg aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc
    17701 ggaacttctt ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt
    17761 ggtgtagcag tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg
    17821 atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa
    17881 ggaatcattt tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg
    17941 cgaactttat tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt
    18001 ttaggaactg tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt
    18061 tatgtcgcat tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata
    18121 ttcccactag ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt
    18181 tctttcctag ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt
    18241 ttcggcattt ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca
    18301 gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct
    18361 aaacgtattt ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa
    18421 ataagaagga accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat
    18481 gtttgatgaa tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt
    18541 ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt
    18601 aaccgtagga gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact
    18661 gtttcccgtt caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca
    18721 atcgttgtga cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat
    18781 gttgtcaatt ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat
    18841 aggtcatctg gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt
    18901 tgatcgttat agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt
    18961 ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa
    19021 cgcacattat actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga
    19081 tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct
    19141 gccataaaac cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa
    19201 gcagcattga tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag
    19261 tttgggttat actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca
    19321 atcattttct tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc
    19381 aatgaatacc cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata
    19441 taatcagaga gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt
    19501 tctttcttat agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc
    19561 tcatttacca acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct
    19621 tgcagcttga attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt
    19681 tgttttatct cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg
    19741 tacgtacata tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg
    19801 tgctcataag gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt
    19861 aaagcattgc gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt
    19921 ctctggtcga gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga
    19981 gttgtgacca ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa
    20041 tttatgccat gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat
    20101 ggcatagttt tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt
    20161 aaggactgct ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat
    20221 agaaaggagg atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag
    20281 attagaattc ccagtaattt caggatcttg ttgggcccac acataatata gctttccttg
    20341 agaagagaaa gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt
    20401 ttctatccaa tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg
    20461 atgatgagat tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt
    20521 tccattgata taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg
    20581 agcctgccat acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct
    20641 taattcaatc acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg
    20701 tttatatacc caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat
    20761 cataacaaat ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc
    20821 cctacattta attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt
    20881 tctggttctt cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct
    20941 ccttgatcta gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga
    21001 ttgatcaaga aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga
    21061 acatttttta tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga
    21121 cctttagcat atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt
    21181 cctttagcat ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc
    21241 tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct
    21301 tccatagtaa agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt
    21361 tccggttggg catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc
    21421 aaatatcctt ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga
    21481 taagcttttg caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag
    21541 tcttcttcat catacttatc aatatagtta tgcaaagcta aataatctac attctcataa
    21601 gcttcttcta atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt
    21661 ccaactaaga ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt
    21721 gctaacctac catattcata agcactctta gcccccactt gccatggacc gtctagctca
    21781 ttccctaaac accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa
    21841 tcactccaga aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca
    21901 attccttttg ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat
    21961 ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt
    22021 ttttttggtc gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag
    22081 tttcctccag gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct
    22141 tgtctaaacc cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg
    22201 acacttccca tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta
    22261 aaatcctctc tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt
    22321 cctatagtca agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa
    22381 ggagggaaaa gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt
    22441 ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata
    22501 tctttgtttt cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa
    22561 actacatatc cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga
    22621 aaataaataa tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt
    22681 ccttgaaaga aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca
    22741 taaaaaacag cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag
    22801 agaataccta ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca
    22861 actattttca tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt
    22921 tctgtgcaaa aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta
    22981 caggtccata aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc
    23041 tcatttttga actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca
    23101 gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa
    23161 aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag
    23221 taataaaaaa ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca
    23281 aacctgcata agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga
    23341 agacttggaa tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt
    23401 tgaaacgcat acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca
    23461 ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc
    23521 aaaccttaat atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga
    23581 taatgtcttt ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa
    23641 ccaacaatat aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc
    23701 gactattcac agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc
    23761 atttgttttt ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg
    23821 ataaacaaaa ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct
    23881 cgactgtaaa tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca
    23941 acatatactt ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct
    24001 aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca
    24061 tccatttgag cagattcata caaagaggga gagattccct gaatagcagt caagagaatc
    24121 gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg
    24181 gttggatcgc gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc
    24241 gcccctacat tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa
    24301 ataactggaa tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat
    24361 gctactgctg ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat
    24421 aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca
    24481 aaacttacaa aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat
    24541 gttcctaaaa gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac
    24601 aaatacgcag cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc
    24661 tcatctttca ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc
    24721 gaacaatcgc tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag
    24781 atatctgtat cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat
    24841 tgattgatgg catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat
    24901 ttttcaatca gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt
    24961 aaatatggct ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt
    25021 acttcttcat cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat
    25081 tttttaccaa tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta
    25141 ttattatctg tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg
    25201 tctgaatcac tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat
    25261 ccgttattta accatggccc attgatttca atggctagtt gaccagcatt cattaaattg
    25321 tccatttcgg cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg
    25381 atcgctttta acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg
    25441 ttcactaatt taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta
    25501 ttgaagacaa aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc
    25561 aattcttccc aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca
    25621 ttattccaat acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct
    25681 acttcaccta attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc
    25741 cagaaatcgt ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa
    25801 tttaatgcaa taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc
    25861 aaattggccc aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta
    25921 aattcattaa cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa
    25981 gtcaaagtca cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct
    26041 aaaatacctg ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt
    26101 tgttcctcct tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa
    26161 acaattgatt ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc
    26221 acttttagga agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac
    26281 tccctctaat gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt
    26341 aactgcaaat atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac
    26401 ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc
    26461 ttgtccatac acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc
    26521 gtttgtttct gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt
    26581 tactctatct gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc
    26641 aaaattatag ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc
    26701 ttgttccgct gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt
    26761 actatgtttc ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc
    26821 catgtctaag gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg
    26881 atgcagagac aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc
    26941 accaaatgta ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc
    27001 tacgagtttc atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt
    27061 atgtccaatc tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa
    27121 agggctctct tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt
    27181 acaatattcg actaaattac gagctgcatc tactccacga gtacccagat tgacagccat
    27241 attgatttcg gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt
    27301 cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat
    27361 actgtcttcc caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat
    27421 gttcaattct tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg
    27481 atgatctggc tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact
    27541 gtacaaccgc ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc
    27601 cataactgac ctcctttaat ttatacatga ataatacacc tcgttgaaa
//
LOCUS       DAJSVD010000028        26393 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSVD010000028 DAJSVD010000000
VERSION     DAJSVD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.028"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(104..1336)
                     /locus_tag="ON498_001060"
     CDS             complement(104..1336)
                     /locus_tag="ON498_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3939352.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ON498_001061"
     CDS             1591..2160
                     /locus_tag="ON498_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3939353.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ON498_001062"
     CDS             complement(2338..2778)
                     /locus_tag="ON498_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3939354.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ON498_001063"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ON498_001063"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3939355.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ON498_001064"
     CDS             3732..4655
                     /locus_tag="ON498_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR3939356.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ON498_001065"
     CDS             4731..5870
                     /locus_tag="ON498_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR3939357.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ON498_001066"
     CDS             5863..6663
                     /locus_tag="ON498_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3939358.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ON498_001067"
     CDS             6663..7490
                     /locus_tag="ON498_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3939359.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="ON498_001068"
     CDS             7468..8202
                     /locus_tag="ON498_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3939360.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ON498_001069"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ON498_001069"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3939361.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ON498_001070"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ON498_001070"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR3939362.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ON498_001071"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ON498_001071"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3939363.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ON498_001072"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ON498_001072"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR3939364.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ON498_001073"
     CDS             11787..13820
                     /locus_tag="ON498_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939365.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="ON498_001074"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="ON498_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="ON498_001075"
     CDS             15353..16159
                     /locus_tag="ON498_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939366.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="ON498_001076"
     CDS             16171..17391
                     /locus_tag="ON498_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939367.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="ON498_001077"
     CDS             17381..18967
                     /locus_tag="ON498_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3939368.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="ON498_001078"
     CDS             19006..21144
                     /locus_tag="ON498_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3939369.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="ON498_001079"
     CDS             complement(21512..22495)
                     /locus_tag="ON498_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR3939370.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIERVAFVEEYLKPFILK"
     gene            22873..24264
                     /locus_tag="ON498_001080"
     CDS             22873..24264
                     /locus_tag="ON498_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3939371.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24280..25320
                     /locus_tag="ON498_001081"
     CDS             24280..25320
                     /locus_tag="ON498_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3939372.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25339..>26393
                     /locus_tag="ON498_001082"
     CDS             25339..>26393
                     /locus_tag="ON498_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3939373.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         8436 a         4420 c         5373 g         8164 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactctc tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataac agagatatta ttttgtgatt
    22861 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
    22921 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
    22981 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
    23041 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
    23101 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
    23161 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
    23221 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
    23281 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
    23341 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
    23401 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
    23461 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
    23521 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
    23581 agtattattg aaacatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
    23641 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
    23701 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
    23761 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
    23821 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
    23881 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
    23941 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
    24001 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
    24061 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
    24121 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
    24181 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
    24241 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
    24301 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
    24361 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
    24421 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
    24481 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
    24541 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
    24601 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
    24661 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
    24721 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
    24781 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
    24841 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
    24901 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
    24961 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
    25021 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
    25081 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
    25141 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
    25201 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
    25261 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
    25321 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
    25381 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
    25441 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
    25501 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
    25561 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
    25621 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
    25681 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
    25741 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
    25801 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
    25861 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
    25921 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
    25981 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
    26041 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
    26101 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
    26161 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
    26221 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
    26281 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
    26341 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga aca
//
LOCUS       DAJSVD010000029        25553 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSVD010000029 DAJSVD010000000
VERSION     DAJSVD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.029"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(213..578)
                     /locus_tag="ON498_001083"
     CDS             complement(213..578)
                     /locus_tag="ON498_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939374.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            786..1268
                     /locus_tag="ON498_001084"
     CDS             786..1268
                     /locus_tag="ON498_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939375.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1465..2355
                     /locus_tag="ON498_001085"
     CDS             1465..2355
                     /locus_tag="ON498_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCR3939376.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2680..5151
                     /locus_tag="ON498_001086"
     CDS             2680..5151
                     /locus_tag="ON498_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3939377.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5254..6990)
                     /locus_tag="ON498_001087"
     CDS             complement(5254..6990)
                     /locus_tag="ON498_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR3939378.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6987..7691)
                     /locus_tag="ON498_001088"
     CDS             complement(6987..7691)
                     /locus_tag="ON498_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939379.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7822..8325
                     /locus_tag="ON498_001089"
     CDS             7822..8325
                     /locus_tag="ON498_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR3939380.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8285..8623
                     /locus_tag="ON498_001090"
     CDS             8285..8623
                     /locus_tag="ON498_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR3939381.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8644..10062
                     /gene="ffh"
                     /locus_tag="ON498_001091"
     CDS             8644..10062
                     /gene="ffh"
                     /locus_tag="ON498_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3939382.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10174..10752
                     /locus_tag="ON498_001092"
     CDS             10174..10752
                     /locus_tag="ON498_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3939383.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10756..11277
                     /locus_tag="ON498_001093"
     CDS             10756..11277
                     /locus_tag="ON498_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3939384.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11356..11910)
                     /locus_tag="ON498_001094"
     CDS             complement(11356..11910)
                     /locus_tag="ON498_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3939385.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12163..12438
                     /gene="rpsP"
                     /locus_tag="ON498_001095"
     CDS             12163..12438
                     /gene="rpsP"
                     /locus_tag="ON498_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3939386.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12450..12701
                     /locus_tag="ON498_001096"
     CDS             12450..12701
                     /locus_tag="ON498_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR3939387.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12826..13617
                     /locus_tag="ON498_001097"
     CDS             12826..13617
                     /locus_tag="ON498_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939388.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13787..14308
                     /gene="rimM"
                     /locus_tag="ON498_001098"
     CDS             13787..14308
                     /gene="rimM"
                     /locus_tag="ON498_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3939389.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14298..15059
                     /gene="trmD"
                     /locus_tag="ON498_001099"
     CDS             14298..15059
                     /gene="trmD"
                     /locus_tag="ON498_001099"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3939390.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15195..15542
                     /gene="rplS"
                     /locus_tag="ON498_001100"
     CDS             15195..15542
                     /gene="rplS"
                     /locus_tag="ON498_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3939391.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15915..16576
                     /locus_tag="ON498_001101"
                     /pseudo
     CDS             15915..16576
                     /locus_tag="ON498_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16655..19882
                     /gene="fss3"
                     /locus_tag="ON498_001102"
     CDS             16655..19882
                     /gene="fss3"
                     /locus_tag="ON498_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3939392.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            19996..20310
                     /locus_tag="ON498_001103"
     CDS             19996..20310
                     /locus_tag="ON498_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3939393.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            20323..20697
                     /locus_tag="ON498_001104"
     CDS             20323..20697
                     /locus_tag="ON498_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3939394.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            20707..21051
                     /locus_tag="ON498_001105"
     CDS             20707..21051
                     /locus_tag="ON498_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3939395.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            21122..22474
                     /locus_tag="ON498_001106"
     CDS             21122..22474
                     /locus_tag="ON498_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3939396.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            22534..23676
                     /locus_tag="ON498_001107"
     CDS             22534..23676
                     /locus_tag="ON498_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR3939397.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            23701..23955
                     /locus_tag="ON498_001108"
     CDS             23701..23955
                     /locus_tag="ON498_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939398.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            24211..25395
                     /locus_tag="ON498_001109"
     CDS             24211..25395
                     /locus_tag="ON498_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3939399.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            25392..25529
                     /locus_tag="ON498_001110"
     CDS             25392..25529
                     /locus_tag="ON498_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3939400.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         8545 a         4496 c         5459 g         7053 t
ORIGIN      
        1 aaaaaataga aaaactagtg atagttttgg tgatagtgat attaaaaatc agaaaagata
       61 gaaaaagcag gagatagata aaatgctgat atttcagcat tttttaagag tataaaagag
      121 aaaaaaagta ataaatgtat acatgatata agaaaaacat atatgtataa aaagaagaaa
      181 gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt tttataagat
      241 aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc tgcaagaagc
      301 aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt aaaattggcg
      361 caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga tataatacgc
      421 ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt gatgaacccg
      481 cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg aacaaaagat
      541 aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca cctaacaagt
      601 gttattattt tgttagtttt attgtaaatt gaatcctgca ataatcaatt actttttcat
      661 tatttctaga ttaaatgaat aaactctcat gccattatct ctaaaaaaaa agtttttccc
      721 cctaattttt ttagatagaa aattgtatag tatgatgaga gaaaaagtag tttgggggaa
      781 cgttaatgag aagaaaagca gtaatcgatc ggctttattt atttttttgg ttttttccta
      841 tcgttttgct accttttaca tgggtaaatg ctgttgtaca aggtgcggag atattaggcc
      901 tgatccaagg aagcggattt gtggtttcta ggaattttcc agctttgact gttttctatt
      961 tagtgataag tgtaggtctc ttattaccaa aatctctgat caatcactac aattggcagc
     1021 tgctttactt ttcttgctta ttagtattta catctatgtt ttctttgctg cctcgcttga
     1081 ttatcggccc taatggtttg agcgagagtg gattgaagga ttcagcttat tcctccatgt
     1141 atttcttgac gatcaaacca tggttctatt tagtaattgc ctcatttatt cttgcaacat
     1201 ttttttactt ctggacagaa atcaggataa aaagtgaacg atctgtatct ataaaaaata
     1261 actattgatg gtggatcatc agtaaaaaag catatgagct gcgtaaaatg catgtttctc
     1321 atatgctttt tttattatta tgtaaagaat gcgaaaaacg tttacatgat ttttacataa
     1381 cttaactata tttttacagc aaccatacga gggatttaca gtcgactgat aaactttagt
     1441 tgtaaataag aaaggagcaa taaaatgaaa aaagtattgt ttactctagg tatgatggga
     1501 attcttttag ccggatgcgg ctcaggatca ggaaatacag atggctctgc cacacaaaat
     1561 aattcaagtg actcaaatga acaagtaaaa atcgttgcgg taggttcgac agcattacag
     1621 ccgttagtcg atgcagcaca agaaagcttt gtacaagaaa atccaaatta tcaaatctct
     1681 gtacaaggag gaggaagcgg aactggtttg agccaagtcg aagctggagc agttactatc
     1741 ggaaactctg atgtatttgc agaagaaaga gacggagttg atgcttcaaa attagtggat
     1801 cataaagtag cagtggtcgg gatggcgcca atcgtcaata aagatacaga tgtcaaagat
     1861 atcaccaaac aggaattgat tgatatcttt acaggaaaaa tcactaactg gaaggaagtt
     1921 ggcgggaaag atcaaaaaat caacgtcgtg aaccgtgcaa acggcagcgg tacccgcgcg
     1981 acattcgaaa aatggggact ggatggtgct acccctgtgc agtcccaaga acaagattca
     2041 tcaggaacag ttcggcagct ggtaagtcaa acaccaggag caatcagcta tttagctttc
     2101 tcatatctcg atgattccac acaagcacta agtatcgacg gtgtagaacc aaaagaagag
     2161 aatgtagcag ataacagctg gggaatctgg tcatatgaac atatgtacac gaacggaaaa
     2221 ccttcccctg aagtccaaaa attcttagac tacatgatga cagaagaaat ccaagaagga
     2281 cctgtcaaag aattgggcta tttaccaatc acaatgatgg aagtagaacg tgatcacgaa
     2341 ggaaatatca aataactagc agataaacat gccacggtag tcgttactat taaggaaaaa
     2401 tcaactatgt ttcccacata tacatttttc ctttaattgt gaaaaatagt tcctcatatt
     2461 ttgccgtttt ttgagctaat accgagggtc agctgattaa acaccgttta ttcgaaaaaa
     2521 agagctacga catagaactg tcgtagctct ttttttgcta tatgacagga atgaaaggtg
     2581 tttgcaagct taatgtgagg aacactttcc atatctgttc tatactggat aggagaagtt
     2641 tggaaatatc cagaaaacga aatcaatcga ggtagccaca tgaagaataa atggataaat
     2701 cgaagtaaaa aaaagaaagt aacacaaaga tcatcagata aagtcatccg tctttttcca
     2761 tcgtataaag aaggattaac ggcagatcaa gtcaaggaac gaatagaaaa aggtgcagca
     2821 aataattcag tcgatcccac attcaaaaca aatcagcaaa tcgtattgga aaacatattt
     2881 acgtatttca atttgatctt tcttatctta gccattttgc tttgtttagt agaatcctat
     2941 aagaacttga cttttttacc agttatcata gccaatacag gaatcgccat ttttcaggaa
     3001 attcgatcca aaaagatctt ggacgaacta aatgttttaa atacagcaaa agtcaatgtc
     3061 atacgggacg ggatagaaca aacaatagat atagaagacc ttgtaataga tgacattgtt
     3121 attttagaga ctggacatca aattccagca gatgcagagg tcgtggaagg aaaactgcaa
     3181 gttaatgagg ctttattgac aggcgaagca gatgaaataa ccaaagaaat aggcgatcca
     3241 ttaatgtccg gcagtttcat tgtttccgga aaagcttacg cccgattaga caaagttgga
     3301 gcagattcgt atatttccca gctgacagtc aaagccaaga caatgggaga aggagaacag
     3361 tcggagatga ttgcttctct taatcaattg atcaaatgga ttgggatcat cattattcct
     3421 atcggtatcc tgcttttttc tcaaagttac ttttttaatg gaaacacgat caaagaaagt
     3481 gtcgttgcaa tggaagcagc attgatcggt atgatacctg aaggactgta cttgctgacg
     3541 acgattgcat tagcattaag tgcgactaaa ctagcaaagc aaagagttct tttacacaat
     3601 atgaaaagca ttgagacatt ggcaagggta aatgtccttt gtgtagataa aacaggaacg
     3661 atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct ctaaaaaaca agaaataatc
     3721 agtcagtcag atcaattgga agcatgcatt gctgactatg caaaagcgat ggcaggagac
     3781 aatgcaacaa tggaagctgt taaagcatat tttacaaaaa ccactgatca atcctatcac
     3841 catgttattc ctttttcttc tgtccaaaaa ttcagcagtg tagcgttggc agacaaggtg
     3901 tatgttttag gagcgcccga aatggttttg cgcgatcaat tgccggaata tgcggaagag
     3961 tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct ttggtgtgta cgatgggatt
     4021 cttgaagaac cagtattgac agaagcagtc acaccattag gctatattct gattgctaat
     4081 cctatcagaa aagaagcaag agccacattt gattacttca aaaaacagca agtagccatc
     4141 aaagtgattt ctggagataa tccgctcacc gtatccaatg tagctgtcca agcagggatt
     4201 tcacatgctg atcagtacgt tgatgcatct aaattatcag aagaagaata tgaagcagct
     4261 gtcgaaaaat atacagtttt tggacgcgtc aagccagaac aaaagaaaat cttcgttcaa
     4321 ttgataaagc gtaacaatac agtagctatg acaggtgatg gcgtgaatga tattctagcc
     4381 atgaaagaag cagactgcag tatagcgatg gcttccggaa atgaagccgc catgcaggcc
     4441 tcacaagtgg tcttacttga ttctgacttt tcacgcatgc cagaagttgt tgcagaagga
     4501 cggcgcgttg tgaacaatat tgagcgatca gccagcttgt ttttagtgaa gaatattttc
     4561 tcctttttgc tctcactgtt ttcagtgatc tttgctctga cctatccttt agaaccatca
     4621 caaatcacat tgattagttt gtttactatc ggacttccat ctttcttgct tgctctagaa
     4681 gaaaacaaaa agcgaatcag agggaaattc atcatgaatg tgatggaaaa agctgtgcca
     4741 ggtggactaa cagatatgat cgtagtcggt gcgttggtga tctgcggggt gactttgaat
     4801 ctaaataaaa cagatgtttc tacagcatct accatgcttt tgattgcagt agggttcctc
     4861 gttctctata aaatctgttc accgctgaat aaattcagaa gtcagatcat tcttttctgt
     4921 gctagcggta actttttttc ggtcatcttt ttgcataagc tgttttcaat caccggtatc
     4981 tcagctgtat cacttttgct gttgagcatt ctatttttct cggcagattc gatttttagg
     5041 catttgacca cattggtaga atatttgttt gaacgtaaaa atgaaaaaga gcaaaagccg
     5101 tttagtcgcg tagctaaaaa atttctatcg atatttaaac gagaaaattg acgaaaagta
     5161 tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct ttcgaagcag gatgagtcat
     5221 ccaactttaa aacgcacgag cagttgctct tttttacatt ttcggaatgg taacggtgaa
     5281 agtcgaacct gttccaagat gactgtctac agtaatcgta ccattaagga gttgcacgta
     5341 gtcttttaca atcgctaacc ctaatccggt cccgcctgaa tgtcggcttc gcgccttgtc
     5401 tacgcggtaa aaacgctcaa agatccgttc ctgatcctca atgtcgatac ctataccaaa
     5461 gtcttggaca gaaaacgata gatcatctgt tgcctgatag cggataataa tctcgctgtc
     5521 gctttttgaa tattggacag cattttcaat cagatttttg ataattggat aaaggatatc
     5581 tgttttagta gtaaatggtt cgttctttcc aatcaatcga atcgtcaacc gtttcttttc
     5641 gatgattggc tgataatttt gaacgatttg ctggaaataa ggttctgtat aaattgtctg
     5701 gatctcgtaa gaattctcgc catcttttga cagctggatg atttcccgaa tcagtttatc
     5761 taaccggatc gcatcttttt gcatgatctc aagaaaagaa gtcagagttt gcgggtcatc
     5821 ttttgcacca tccagtaaag tctcggtaaa accaatcagt gaagtcacag gagtttttaa
     5881 ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt tctaaacgtc gcactttcgt
     5941 taagtcatac gccaccccta gaatctgtcc ggtaccttct gaattagtaa agtagcgcaa
     6001 actcaagtct aatgtctgcc gaccttctgt aagggtgatc tcctgatgga tcagcggatg
     6061 ctcaggagtc acttgatgaa tcagctgaat gagttttggc tcttgaatga cttctgtgta
     6121 ccgctgttca ggttgatagc ttaccacgtt taaatgtagc tgcatctttg gattaagcag
     6181 tagtaaacga gaatcagcat catcaatcag gaatacgcca atcatcagtt cgttcagtaa
     6241 cgtatataat tgctcttctg tggtagtaca cgcccgatac atcttactca tttgttcgct
     6301 caacgcatta atcgtctgat acaattcttc ccattgatcg ggtgtttgca tgattacttc
     6361 cgcctgatta ggattttgca ccattttttt tagtacgggc agcacggttt tcaatggctg
     6421 gtttttttga tagatcagat aatagatcat tcctgcaaga acaaggaaga aagacagaaa
     6481 gaagaaaaag acccaacggc gaaaactctc agttctcggt aaaaaaccgc ttgtcggttc
     6541 tgcgatacgt aaaatggctt ctaactggcc actttttttg actggtaatg cgacatataa
     6601 aagatcctca ttcaaagtag tacttttgcg caaagcgcta cctaggttcc caccattcaa
     6661 tacggctttg atttcgggac gggagtttcg ctgttcattc aatgtttcat tgtttgtgtc
     6721 aaataagatc tttccagtag catccattaa agtaatccgt tcgttcgact gatggacgaa
     6781 ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt tcgacatcta attgccggat
     6841 cagcagttgt gctttttttg tcaggtagct ttcttgctgt tccacgatct gctgttgaaa
     6901 ataatgggaa atcaattgcc agccaccaaa aaacaaacta actaaaagaa taagaaagaa
     6961 gagacgttca taaatttttt tccatttcat cgttttggtt cctgaaactt atagccaaat
     7021 cctctaatag tcaccaagta ctttggatgt tttggatctc gctcgatttt ttctcgcaaa
     7081 tgactaacgt gtacatctac gattcggctt tgtcctgcga agtcaaaatt ccaaatgcgg
     7141 tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt tcatgaaata aacaagtaat
     7201 tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta cggtcacttg ataattggtc
     7261 aaatcagcag tgatttgtcc aatcgataga tattcaggct cagcttcatc ttttgattgc
     7321 ttacgaggtt caatccgtct gaatattgct ttcatacggg ctaatacttc gcgaggacta
     7381 aatggtttgg tcaaatagtc gtcagcacct atttctaggc caatgatccg atccacttgg
     7441 tcatctttag ccgtcagcat caaaatcggt gtttcatttt tttcttgacg cagccgctga
     7501 gtgatctcca tgccatccat cgttggaagc atcacatcta aaatgatgaa atcaaatgat
     7561 tgttctaatg ctaattctaa tccttcgcct ccatctgtgg cactggttac cttgtagcct
     7621 tctttttcca aattgaacgt caagagcgtt acaatagaag gttcatcatc aacaactagt
     7681 atttttttca ttcaaatctc ctttaaatag gtttcattct gtttatattt tagcatagaa
     7741 tcagtacaaa gggctatttt acaaaagaaa taaatggtat aataggaagg cactttgctg
     7801 aatagaaagg aagagcagtc aatgattgga atcagtgcat gtttaggcgg tctggcctgt
     7861 cgctatgatg gacagaaaaa agaagtacca gaattgatgg aactagttga aactggagaa
     7921 gctgtcatgg tctgtccaga agtgatcggc ggattaccaa ttcctagaga tcccgctgaa
     7981 atcattggtg gaaatggatt cgatgtgtgg aaagatcaag caaaagtatg gacgacaagc
     8041 ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca aagcctatca aaaattgcag
     8101 gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc cttcatgcgg aacccattcg
     8161 atttatgacg gaagtttttc cggaataaaa aaaaatggtg ccggagtggc tactgcttat
     8221 tttattcagc aaaaaatgac tgtgctctct gatgaagaat ggctgaaaac tagaggtgag
     8281 cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc tttgaatttt attcgacctt
     8341 attgacagaa aaacaaatga actacatgga attatattat gcagatgatt tttcattagg
     8401 tgagatcgcg gaagaatacg aagtaagccg acaagcagta tatgacaata tcaagcggac
     8461 aagcaagatc ttggaggatt atgaaaaaaa actccatctt ttttctaatt atattgttcg
     8521 agaacaggta ttggaaaata tgactgcata tattacagat aagtacccgg aagataaaaa
     8581 actacttgaa tatgtacagc aaattcaagc aatcgaagaa taaataaaag gaatgaagaa
     8641 aatatggcat ttgaaagctt aactgaacgt ctccagcagg cgatgggaaa aataagaaaa
     8701 aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc gggaaatccg ccttgcctta
     8761 ctagaagccg atgttaactt gcaagtagtc aaagatttta ctaagcgcgt acgcgaacgt
     8821 gcggtagggg cagaagtgct ggaaagttta tcacctgcac aacaaatcgt aaaaatcgtt
     8881 gatgaagaat tgacgatcac acttggttct gaaacagcag agttaaataa atcaccaaaa
     8941 ataccaactg ttatcatgat ggctggtctt caaggggcag gtaaaacgac ctttactggt
     9001 aaattagcca attatttaaa gaaaaatgaa aatgctcgtc cgctcctaat cgcaggggac
     9061 gtatatcgac cagctgcgat cgaccaattg aaagtattag gtcaacagct agacgttcct
     9121 gtatttgata tgggaactga agtcagcccg gtagagatcg tccgtcaagg gatggagtta
     9181 gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg caggacgtct gcatatcgat
     9241 gaaacattga tggatgagtt gaaacaaatc aaagaattga cgcaaccaaa tgagattttg
     9301 cttgttgtcg atgccatgac tggtcaagat gccgtcaacg tagctgatag cttcaaccag
     9361 caattaggta ttacaggggt cgtcatcact aaacttgatg gggatactcg aggcggggca
     9421 gcactatcga tccgctcagt gacaggcgca ccaatcaaat tcatcggttc tggtgaaaaa
     9481 ttgactgact tagaagtatt ccacccagat cgtatggcta gccgtatcct tggtatgggg
     9541 gatatgctga cattgattga aaaagcgcag caagactacg acgagaaaaa agcggaagaa
     9601 cttgcacaga aaatgcggga aaattcattc gatttcaatg actttattga acagcttgat
     9661 caagtgatgg gtatgggccc actggaagat ctaatcaaaa tgatcccagg aatgaatcaa
     9721 gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg ttgaacgcaa aaaagcgatg
     9781 gtttattcta tgacgccagc agaaagagca aatcctgatc tgttgaaccc aagccgtcgt
     9841 cgccgaattg ctgccggttc tggaaacagt gttgttgaag tgaatcgaat gatcaaacaa
     9901 ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag gggatatgaa catcccaggt
     9961 atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag gcaaaatggc gatgaatcga
    10021 atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat aagatgaaaa aaacagcttc
    10081 ggtacagact gaaaatgtct actgccggag cttttctatc agcaattttt attctttact
    10141 aatacaatta ctctaaacga agaggtggca cagatgagat gtccgtggag tgaacaaaca
    10201 gaaagtatgc ggcaatatca tgatactgtc tggggtgttc ctgaatatga tgaccaaaag
    10261 ctgttccgaa aattgatgct tgatattaat caagccggat tgagctggca gacgatttta
    10321 aataaaagtg aagcattcga tgaagcatat gatcgattcg atatcaaaaa agtcgcaaat
    10381 tatggagaaa caaaaatcag tgaactaatg gaaaacaaag gaattattcg taaccgaaga
    10441 aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga aaattcaaga agaaatcggc
    10501 agtttttcag agtatctttg gtcgttttct aatggaaaaa tcgttgattc ttcttatcat
    10561 tcacaagaag aagtcccaac aacaagtcct ctttcagatg aattagcaaa agatctaaag
    10621 aaaagaggat ttaaatttat cggaagcaca acaatctatg cttttttaga agctgtcggg
    10681 atcatcaatg atcaccttga cagttgtttc cgcaaagaag aggtcgtacg tatcggcaga
    10741 aagggcgaat aaagcatgcg aaaaatcata ttttatggtg ctataagttt agatggctat
    10801 ttggcaacga aaacggatga tcttcaatgg ctttttgaca ctccaacagg taagcaaact
    10861 acttatgaag ctttttatga aacaatcgat acgaccatca tgggcagaaa aacttatcag
    10921 gaagcaaaaa aatatttgga gaccgaagca atttatccag agaaaaagaa tttcgttttt
    10981 tccacccacg cacaattaca attgccagat gctgaggtcg taaacgaaga tcctgttgct
    11041 tttctaaaaa atctacaaat gacagaagga aaagatatct gggttgtggg tggcggtcga
    11101 ttgatcaaac cgctattgga acatcaaatg attgatgaat ggtatatcca gattacccct
    11161 gtattattag gggacgggat tcctttattc caaaaaggaa catatgaagc gcgttttgaa
    11221 atgctagaca cgacacgatt cggtgaattt atcgaattgc attatgtacg aaagtagaag
    11281 taaataaaat aaaaaaggca actatatgca gaaatgtcct gtacatagtt gcctttttta
    11341 ttaccgttat gctttttact ctgttaattt gttagaaaaa tctagatacg catctaaaat
    11401 cgtttggaag aaacttaatg ttccttcaac gatattacca ttttcatcaa ttagattcgc
    11461 aacgtttccg atatatgctt ccggttgctg caaggttggc acattcaaaa agacaagtga
    11521 ttggcgtaga tggtggtttg cgccaaagcc gccgatccca cctggtgatg cagtaacgac
    11581 taaacctggt ttatgatccc aaacactgtg tccataaggt cttgagccta catctaaagc
    11641 atttttcaat actgctggca cagaacggtt gtattcagga gtaacgaaaa cgacaccgtc
    11701 tagtcccctc acttcctcac gaaaacgagt ccattcagca ggagcctgtt ctggagtttc
    11761 gagatcctca ttgtaaaatg gcagatcatc gatttttaca aatacacttt cgtacccttc
    11821 aggaagcaat ttcgcaaaag cttctgctac ttttttgttg taagaatctt ttcttaaact
    11881 accaataaaa aatccaattt tttttgtcat acgatttttc ctccttcaaa ctatctataa
    11941 taatcttaca aaagataagt aactggagca aataaaaaca cttactgaca aaagctacat
    12001 agaagataga ttacttattt ttaacaaaaa agaagtctaa aatcttctat tttctaaaaa
    12061 aaatcgaatt taatcaagct gtaaagaaaa aaagctttac agggtaaaaa aaaactgtta
    12121 cactacatct tgtgaataaa acaatggagg tggaaataaa taatggcagt taaaatccgt
    12181 ttaaaacgta tgggttctaa aaagagtccg ttttaccgta tcgtcgtagc tgattcacgt
    12241 tctcctcgtg atggacgttt catcgaaact gtaggtactt acaatccttt gaaagaccct
    12301 gcagaagtag ttttaaaaga agacttagtt cttgactggt tatcaaaagg tgcacaacct
    12361 tctgacactg tacgtaacat cctttcaaaa gaaggcgtta tgcaaaaaca ccacgaagct
    12421 aaattctcaa agaaataagg tgaccggata tgaaagattt aagcgactta gtcttaacaa
    12481 tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt agacgtcgta gagtcgaaag
    12541 acttttacga gtacaatcta acagtggctc ctgaagatat tggccgtatc attggaaaac
    12601 aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg tgttcggatc aatgcaccga
    12661 aaaaagtgcg tttgaatatc atcgatgata aggaaaagta aataaacgtg ctgtactcga
    12721 attttcgagt gcagcttttt tgtttttttt attaattgaa gaaatgatta tttggcaaaa
    12781 caagcaagaa agcgtatcct tataggagga gggagtgaac agccaatgag aaagaaaatg
    12841 ttatttattc catttgctct attgttaagc gcttgtagtt cggttactca agaatcagac
    12901 aatacacatc tatcaagcag tatatctgaa actgttacca gtccagaaaa aagtagtacc
    12961 aaaacggctt ccaccactga acaaacaatg gctacctcaa ataatgagaa aaagactgct
    13021 ttagaccagt tgaaagaaca acagccaaat gttcccatgc ctctagatgt accagtctct
    13081 tcaggctatc tcaatatcgc agcgacacat acaaagcaag gttattctat tttatattac
    13141 agaacagatc gtcccctcgg gttgaatgct gacgaattga atcaagaaac gcctattgca
    13201 acctatctat atcaatatgg gtttgcttct agtcaagaaa caatccaagt tttgcagcca
    13261 tttgagatcg acacaaatgg tcagcaagtg gacttaggtt ctcgaatcac agggtatcag
    13321 caaggtgcag caggttcaag ttttcttgaa tggcaagaag gaaactggcg tattcgaatc
    13381 cgtgggaata atatagaagg ccaagatccc ttactattag ctaaagaaat cgtagcttat
    13441 ttggaagaaa acagtctacc agcacctgaa caatttggta aaattactgt tgatatgggt
    13501 gatacgacga atcgagccgt agaagtaagc tggcaagaac cgaaaaatgc ctatacgatc
    13561 actcatcaag atcctatgtc tgcgttgaag atggcagttt ctatgaagag gttgtgaaat
    13621 cagcgcgctg atctgagccc atcatttaac tccaagtcac aggaagtaat caaaaatagc
    13681 acgccgcatt tttgcataat tttggcttaa taccttaaaa gaaaactaga attgcctctt
    13741 cacacatgct aaaataaaac agacaaaaaa gaaatgggga tataagttga ctgaatactt
    13801 gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc gaagtccgtg ttatctcaac
    13861 aacggatttc ccagaagaac gttacaaaaa aggaacggtg ctaacactat tccaagacgg
    13921 aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa cataaaaatt ttgacttatt
    13981 gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa tatcgggatg gcatattaaa
    14041 agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa ttctattacc atgagatcat
    14101 cggtctgcat gtattagaag atggaactga gttaggtaaa gtaaaagaaa tcttgtcacc
    14161 aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa gctgatatat tgcttccata
    14221 tatcgcttct gtcgtcaaag aagtcgattt agaagctgga gtggtccgtg tagaaattcc
    14281 agaaggactg attgacgatg aagattgatg tattgacttt atttccaaga atgtttgaag
    14341 gtcctatggg ggaatccatc atcggtaagg ctgtagataa aggattatta gatatcaata
    14401 tctctaactt ccgcgattat tcagataaca aacatcagac agtagatgat tatccatacg
    14461 gcggtggagc aggtatgctg ctcaaagtcc agcccatcta tgataacatc caagccattg
    14521 aaaaagctgc accggatgtc aaaaaaagag taattcttct tgacccagct ggtaaacgat
    14581 tcgatcagaa aatggcagaa gaattttcac aagaagaaca cttagtattc atttgtggtc
    14641 attatgaggg atatgatgaa cgtatccgca cattagtgac agatgaagtt tccttaggag
    14701 attatgtcct tactggggga gaattaggtg cgatggtcat gatcgatgca acagttcggc
    14761 ttttgccaga tgtgttaggg aatcagactt cagctcaaac ggattcttat tcaaccggtt
    14821 tattggaaca tccccaatat actcgcccgg cagaatataa agggatgaaa gtaccagaag
    14881 tattgacaaa tggaaatcat aaattgattg aagaatggca attaaaagaa tcactaagaa
    14941 ggacctatca acgtcggcct gatcttttgg aaacacttga atggacacct caaatgacga
    15001 aatttttaga agaaatcaag aaagaagaac aagaaaaaac agcagatgaa gccaagtgac
    15061 tttttctaaa atcttcttta cagtaggttg ataatatgtt agtattttat ggtgagtgca
    15121 aaagcactta actattacga tattccgctg tgagaaaagc tcataagaat atttggaata
    15181 aggagaagaa aaccatgaat ccattaatcg aagaattaac aaaagaacaa ttacgttctg
    15241 acatcccagc gttccgccct ggtgacactg tgcgtgttca cgcaaaagtt gttgaaggta
    15301 ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa acgccgcggt gctggaatca
    15361 gcgaaactta tacagtacgt aaagtttcta acggagtggg cgttgaacgt acattcccat
    15421 tgcatacacc acgtgttgca aaaatcgaag tagttcgcta cggtaaagta cgtcgtgcaa
    15481 aattgtacta cctacgtgca ttacacggaa aagctgctcg tatcaaagaa atcagaagat
    15541 aaacacggaa aacttccttg gaaatctgaa atttgtgaaa agtccttgta tatcaagggc
    15601 tttttctttt ttctctatca cagttttaag ccatttttgg ctgttttgga ccatttttca
    15661 atcatattcc cttaaaaatt gtcctagatt gtccgacatt gactatcagt gtcgtcaaaa
    15721 tagggcaaaa tttagtagtc agcttgacga caaatataat acgaaaaata ccaaaacatt
    15781 taccgaattt agcaactttt gaacccaagt ttctcaaagg gtcagctatt agcaaaaaag
    15841 acaaaacatg tacttttcta ctaaaaagtt gatgtgatag gcttctatct aaataactat
    15901 taggagtttt tatcgtgaag caaaatgatg gacgtatcat ttggaaaaat caaagtgagt
    15961 taaaactgat tttaacaatt aatgaattca tcgaaaaaca cggaattaca tctagccgtc
    16021 aataccagaa aaagctatca gaaaatccaa attctgcacc aagtatgtgg ttcattaaca
    16081 aaaaatatgg atcatgggag aatttattga ttagtatagg acgagaaaat actgggtatg
    16141 gaaaatgggc tagaatgtca gaacaagaat tattggaaat agtagaagcc tttattaagt
    16201 gcgagaagat aacctctcaa cgaatgtacg aacaaaaatc agtagggaag aatattcctt
    16261 cacttagtac cattaaaaag atgttaggag atatacgtcc actttttaaa gaaaaaaatg
    16321 atggatctcg atttactgat ttcgaactgc tgttagaact aaaaaatgaa ataatccgat
    16381 taaaactaca ggatgattta tcaatgacaa agtttcgaaa actagttcaa tcccaaaact
    16441 accatcagtt gatacaatta tgaaaagaac gaacaaaaat tgggaggaat tgatggcaga
    16501 gattggattt gattatcgaa gaatcaaaat ttataagcag agaaacaacc tatccaagac
    16561 aaagaaaact aaatagtatc actctaaatc agacgttcaa aattaaaatt tggacgtctt
    16621 ttttattacc caaaaacgaa aggagattta ttttatgaaa aagccaagat ttaagaattg
    16681 gcgtttgctc gcaacactga gtctattatg ccaaacaatt ggtggatcgc ttggcccaac
    16741 gattgccttt gctgatgaaa ttactcatcc acaaacggta actgtggaat tggatttggc
    16801 tcaccagtat gctgtcgaag gtacatttag cgatggtcgt cccatgtcag aagttaccgt
    16861 tccgcactat gcagtttaca atggtgtgaa gcaagatatt ttctgtattg agccaggggt
    16921 gccgatttac aatgagttca ctcctggtta tgagaaaaat ccattgcctg atatgtcgga
    16981 gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt acggatgtgg atacacatat
    17041 tgtggcccag aagatgattt ggcaagaagt taatggctac acgcttcact caattaaacg
    17101 ttctgatggt agtgcagtaa atatcgcagc cattgagtca aaaatcaatc aagccattgc
    17161 ggattatcaa aagaaaccaa gcttccataa ctccacagtg aaaaccgtgt taggtcaatc
    17221 gactactgtg acagatacga acggcttgaa tttatcggag tttgatgaag ttgtcgagaa
    17281 tatcgcaaat attgattatc gtgtgaacgg caatcaacta gtgattactc caaatgccaa
    17341 ttcaaaagag agtggtgaat taaccctaaa gaagtcagct aatacaggaa caccggtagc
    17401 ttataaaatg gctggtcaac aaactttgat ggcgggggca attgataagc ccaataccta
    17461 cacaatcaaa attgatgtgg aaactgaggg ttctttgaag attaaaaaag tcgataaaga
    17521 aacaggaaat gttgtaccgg gaaccgtttt ccatttagac tttggaaaaa cattacctgc
    17581 aaaagacgtg accaccgata aagaaggcat tgcgacattg gatgagattc ctcatggcgc
    17641 gaaagtaacc attactgaaa aatcagtgcc agctccttat acgattgaca ctacaccaat
    17701 caccgcaacc attaaatctg gtgagactat ttctgtgact tcaaaaaata ctcgcgaaaa
    17761 aggtcaaatc attttgaata aaatgggggt ggaaacagga actgatcttt ggaacgacaa
    17821 atactcatta gccggaaata cgtttgccat tcgcaaagac agtccaaccg gtgaaattgt
    17881 ccaagaaatg accacggatg aaaaaggtca tgcggaaaca ccaaaagaga ttgccaatgc
    17941 gttggaattg ggaacctatt atgtgaccga aaccaaagct agtaatggct ttgtgaatac
    18001 ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa accgtggctc ttgttaccag
    18061 taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc actttgacaa aagaagacaa
    18121 agataccggt aatgaaactc aaggtaaagc tgagtttaaa ggagctgaat atactctctt
    18181 tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct tttaaaccag agttagtgaa
    18241 gggaacgaaa gcttctgatg aaacggtgac tttgacttta gatgaaaaga atcaagttgc
    18301 tgttaaacac ttagcgatta atgagtatta ctggcaagaa accaaagcac ctgaaggata
    18361 taccttggat gaaacaaagt accctgtttc catcaaaaag gtggatgata acgaaaaaaa
    18421 tgccgtgatt acccgagatg ttacggcaaa agaacaagtc attcgctttg gctttgattt
    18481 ctttaaattt gctggatcag ctgctgggac tgccgaaact ggcttcaatg atctgacctt
    18541 taaagtatcg ccattggaag gaaccagtga gattacagat gccgaagatg aagcaattac
    18601 agcttataac gagcaactag gttttgatgg ctatggcaag tttgaaaatc ttccttatgg
    18661 ggattattta cttgaagaag tagaggctcc agaaggattt caaaaaatta cgccattaga
    18721 aatccgttct acttttaaag aaaataaagc ggattacact aagagtgaat acatttttac
    18781 gattaccgaa aaaggacaaa aacaaccaat taaaacggtg accgttcctt acgagaaact
    18841 gacgaataaa gcattttctg ttagcttgaa tcgtttgatg ctctatgatt tacctgaaga
    18901 agaagatagc ttaacttctc ttgggacttg gaaagacgga ggcaaagaat tgacagacct
    18961 tgattctacc gagctagttg ataaattgag ctataacttg catgaaatca aagaagactg
    19021 gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa gctgcccaag aaaaagatga
    19081 aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg ttggcgaata aagagaaaac
    19141 aggaacttgg aaaattttgc ataaattaac cgcagaacaa gttttggata aaaccatcgt
    19201 cttattcaac tatgtgtatg aaaacaagaa accctatgaa gcaggtgatg atccagtagc
    19261 gaaagatgct agcttgaaca atcaagccca aactgtcaat tgtacggtgg aacgccatgt
    19321 ttccatccaa acaaaagccc acctagaaga tggttcgcaa acctttactc atggggatgt
    19381 ggtggatatg ttcgatgatg tgtcgattac ccatgatgta ctggatggct caaaagaagc
    19441 ttttgaaaca attctgtatg cattacttcc ggataatacg accaaagaaa tttggaaatc
    19501 tggcaaaatt gattatgaag tgaatgacaa agaattcact aaaaccgtac ttgcggaaaa
    19561 agtggacaca agtaaatatc cagaaggtac aagttttacc ttcaaagaaa ttaactatgg
    19621 caaagacgga aacatcaatg ggaaacacaa tgaggatcta aaagaaaaaa ctcaaacttt
    19681 aacacctaaa aaagttccga caacaccaag tgcaccagaa caaccggaaa taccaactgt
    19741 tccaagtgac tctcaagaat ctagtcctac agtaaagaca ttcccacaaa caggggagaa
    19801 aaattctaat gttctgctgt ttattgggtt taccttgatc tttgcgactg ctgggtatta
    19861 tttctggaat cgccgaaact aaggtgatgt gatgaaacaa ctaaatagga aaaaccaacg
    19921 tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt gtatccaaaa ctaaaaatga
    19981 aaagaggaaa agaagatgga attgaaattt gttgtgccag atatggccga aacctttgga
    20041 aagataaggt atgccggcga aggcgaagtt ttaactgaag gatatgggcg gaatactaca
    20101 gtaattggtc gtagttatca tttgtattcc agtaagcaac gagccgatga tattgaagtg
    20161 gtggtagctg cggaagccgg cgaaaaggat tttgatcaag atcaaccact aaaagccgtg
    20221 aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac cgactacgtg
    20281 ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt
    20341 attgttgtag attcaggact aacgtttggg aaattacgat tttccgcatt acgccgagaa
    20401 gtacggaagc aaaatgagga tggaacggtt agtaacgagg taaaagaacg gacttataat
    20461 ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta tcccagcaaa tgtaccctta
    20521 cgcgagtttg cctatgacgc agaggtagag ttggtcaatc caatcatgga tacggtcgcc
    20581 aattatgttt tccgagaagg aactactgtc aattggttca ttaaggccga tgatttagtc
    20641 ttgaaacgac aaccagatca gggaaattct acaaaccaaa atgaaggaaa gaaataggtg
    20701 aagcgtatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggt
    20761 gagctaacgg aggaacaact cactaaagca ttaacctatg atgaaaaaat gtttctgatt
    20821 atgcatcaag ggttactgga agtaggtaac gatgccgaag aagattttga tgtactgaat
    20881 tggttagggg aagaagagtc cttctttatg gttatcgaag taaatgaaag tctatgtcga
    20941 gaagccgaaa ccgtattgga agaaattggt gtggaaatgc cggatgccat tgaagttttt
    21001 ttaaagcaac tagtggaaac gaaacaactc cctatagcag taaatgacta gcataactgc
    21061 ttgaaattgg atcaatgaaa tcctatgaag cttttttcga ttggaggtgg aaaccataaa
    21121 aatgtacaaa gggcatcgta tacgggcagg agatcagcac ttggtttatc actttgttct
    21181 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagcttag
    21241 acaatttgat atttctaagc tatcactttc tactatagaa atcgttcgtt ctataaaaga
    21301 tttaatctat ttgctaggaa gtctcgttct ttcaggaagc acgatgttgc tttatataca
    21361 cttttttcag gatcattggc gcagtttgtg gcatcgacaa aagctagccc ggatgattct
    21421 agaaaatcat tggtacgaag taaaacaaac tcagagtgaa ggatttttca aagacctcaa
    21481 tagtagtcga accaaagaga caatcagtta cttccccaaa atctattacc gtatgaagga
    21541 tggtttactt tctattcgcg ttcaaatttc actgggaaaa tatcaagatc agcttttaaa
    21601 gctggaaaag aagttagaaa gtggtctata ttgtgaacta gtagaaaagg aactcaaaga
    21661 ctcctatgtg gagtacactt tactgtatga tataattgcc aatcgaattg ggatagacga
    21721 agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat caagtatggg cttatgattc
    21781 tttacctcat atgttaatcg ctggcggcac aggtggtggg aagacttact ttttactcac
    21841 tatcatcgag gctttattaa aatctgatgc cgagcttttt gttcttgatc ccaaaaatgc
    21901 ggacttagcc gatttaggta cggtaatgcc tcatgtctat tcccaaaagg aggaaatttc
    21961 tgcttgtgtg gaagatttct atgagcgcat gatgactcgt agcaaggcaa tgaaagaaat
    22021 gcccaactac aaaacaggag agaattatgc gtttcttgga cttccaccaa actttttaat
    22081 ctttgatgaa tacgtggttt atatggaaat gttaacgacg aaggaaagtg cggtgatttt
    22141 gaataagctc aaacaaatcg tgatgttagg tcgtcaatct ggtttctttc tgattctagc
    22201 ttgtcaaaga ccggatgcga aatatttagg agacgggatt cgcgatcaat ttaatttctg
    22261 ggtggctttg ggtcgtatga gtgaactggg atattccatg atgtttggcg aggttgataa
    22321 aaaattcttt atgaagcgta tcaaaggtcg aggctatgta gatacgggag ggagtgtgat
    22381 tagtgagttt tatacgccac ttgtaccaaa aggatatgat ttcttaaggg aaattagcga
    22441 cattggtgga ttaaacgtta atacggaagg ataaaataat agtatgtagc ctaggagtgt
    22501 caagaatcct gggctttttt tacgaggtga tttatgacat ttttagattt attcgctggg
    22561 attggtggct tttgcttagg tatggaacaa gcgggtcacc agtgtatcgg cttttgtgaa
    22621 attgatgatt ttgctcgcca gagttataag gcaatccatg atacaagtaa ggaggtggaa
    22681 atgcatgaca tcacaagtgt atcagacgag tttattcaat ctctcggacc agtggatatc
    22741 ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg gaaagcggca aggattttcc
    22801 gatactcgag gtactttatt ctttgaaatc gctcggttcg ccgctattct ccaacctaag
    22861 tttttattcc ttgagaacgt ccggggatta ctcaatcacg aaggaggggc tacgttcgag
    22921 acgatcctcc gaacgctgga tggattgggg tatgatgtgg aatggcaagt gcttaactca
    22981 aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta tcggacattc tcgagacgca
    23041 tgtaccgaac aagtatttcc tatcattgga tcgtctccaa catctgatca aaacatcaga
    23101 aacttattaa atatcaaccc ttccaatcga ggaatggggg gacaagtcta tgggtcagat
    23161 gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa tcgcattgcc ggtagatgat
    23221 ggaatttctg tagcgggtat gttactggga aattttgaac aaggcaatcg ggtttatgag
    23281 ataactggaa cagcgcctac tttgtcaacg aaacaaggcg gaacgaagat catgcttcgg
    23341 aaaaacgaaa cagaatatca agaggtgaag ccagggaaca gtgtgaacct ggcttttcct
    23401 aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg tgcatacctt attaacagga
    23461 gaccaacaag cagtcgtcac ggatcagtat caaattcgaa agttaacacc tagggagtgc
    23521 tggcggcttc agggctttcc ggactgggct tttgatcgag cttctcaagt caattcagat
    23581 agtcagttgt ataagcaggc tggaaattct gtaacagttc cggtcatttt tgatattgct
    23641 agaagattaa aggaggtaaa aacagatgat ttatgatgag ttgattgggg aatctactgg
    23701 gtgattggca agattcagtc agatccggaa ttggaagaag aactgtaccg gctcaatttt
    23761 gatattcgca aaaacggggt gaaagtccct gatgatccgt atgtaattga tgaggaaacg
    23821 gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg aacgaattgc ggactttgct
    23881 aaagaacctg atatccgcca atacttgttt gagataaaag cagagttgga aattgagggt
    23941 attaccgcgg agtaacggcc aaataaacca ctgataggca ctgaccgcgg gggatttgcg
    24001 aagcagaaat cctccgtggt gctgtgcctg tttatggacg gcgttagccg gccatgatgt
    24061 ctcacccccc gtatctaaca ggggggtaca aaaacacaaa aaataagcaa gcaacgataa
    24121 ggaatggctg tcctatcaag gataataagc aatttgtgta gatgttaact gatttgaaaa
    24181 agttaacacc ttaaatttgg aggtgagaac ttggcacaaa ggaatttaga ttatcggcta
    24241 ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata aattagctac gacttgtgga
    24301 cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg taaccgcatc gacaaaaacc
    24361 atgaggaaaa tttttgacca actggaaagt ttcaatcccg agttaccttt aacgttactg
    24421 tttgactatg taaggattcg ctttcccaca acggatgcga ggaaaatcat tcaagagatt
    24481 ctccatttga aatttgatta tatgctccac gaagattacg ccttttactc ctatcaagaa
    24541 caatatgtta tgggagatat tgtggtgatg ttgtcacatg aagaagataa aggcgtcctt
    24601 ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt ttttactcgc ccaaaagcgt
    24661 agctggtatg actttttcga agattgtcta aaagccggtg gcgtgatgaa acgcttagac
    24721 ctggcaatca atgatcgagt tggcctgtta gatattcctg atttaacgaa aaaatgtcag
    24781 aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct atcgttccgg ggaactgttg
    24841 aaacctgatg ataaagatgg tatgggcaat actttgtata ttggcagtct taagagtgag
    24901 gtctatttct gtttgtacga aaaagactat gagcaatata tcaagttagg gattccttta
    24961 gaccagaccg aaacgaaaaa tcgttttgaa atccgactga aaaatgatcg agcttatcat
    25021 gcaattcaag atttgttaaa aggtcgcagt attgaaagta ccacattttc cattattaac
    25081 cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc gaactaactg gcccttgaat
    25141 gaacaatggg ggcgcttcat tggacggaat cagaaggaaa ttcaattaac ttctgaaccg
    25201 aagccttata ccattgaacg aacgttgaac tggcttggac gacaagtcgc ccctacgtgg
    25261 aagatggcca aggagttgga tcggttgaat cagacaacgt acattcagga tatggtaaat
    25321 aatactcgac tatcagaccg gcacaaaaag attttggagc aacaaagtat ggcaattgaa
    25381 aatttgatta tatgaaaaga ggaatcgtga tggaaattgt gaaagatatt ttattggtgt
    25441 ttggtggcag tatgcttggt gttactgtga tgtgcttgat gcatgcaagt gcagcggcgg
    25501 accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt tcggtcaaaa ttt
//
LOCUS       DAJSVD010000030        25510 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSVD010000030 DAJSVD010000000
VERSION     DAJSVD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.030"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            133..3660
                     /locus_tag="ON498_001111"
     CDS             133..3660
                     /locus_tag="ON498_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR3939401.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ON498_001112"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ON498_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR3939402.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ON498_001113"
     CDS             7448..7789
                     /locus_tag="ON498_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3939403.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ON498_001114"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ON498_001114"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3939404.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ON498_001115"
     CDS             9146..11566
                     /locus_tag="ON498_001115"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3939405.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ON498_001116"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ON498_001116"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3939406.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ON498_001117"
     CDS             13000..13737
                     /locus_tag="ON498_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3939407.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ON498_001118"
     CDS             13751..14566
                     /locus_tag="ON498_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3939408.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ON498_001119"
     CDS             14579..15226
                     /locus_tag="ON498_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3939409.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ON498_001120"
     CDS             15242..15898
                     /locus_tag="ON498_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3939410.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ON498_001121"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ON498_001121"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3939411.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ON498_001122"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ON498_001122"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3939412.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ON498_001123"
     CDS             18240..18758
                     /locus_tag="ON498_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3939413.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ON498_001124"
     CDS             18865..19365
                     /locus_tag="ON498_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3939414.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ON498_001125"
     CDS             complement(19645..20565)
                     /locus_tag="ON498_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR3939415.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="ON498_001126"
     CDS             20730..21743
                     /locus_tag="ON498_001126"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR3939416.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ON498_001127"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ON498_001127"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3939417.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ON498_001128"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ON498_001128"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3939418.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ON498_001129"
     CDS             complement(23383..24144)
                     /locus_tag="ON498_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3939419.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ON498_001130"
     CDS             24333..25427
                     /locus_tag="ON498_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3939420.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8449 a         4424 c         5385 g         7252 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt
//
LOCUS       DAJSVD010000031        25404 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSVD010000031 DAJSVD010000000
VERSION     DAJSVD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.031"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ON498_001131"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ON498_001131"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="ON498_001132"
     tRNA            207..279
                     /locus_tag="ON498_001132"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="ON498_001133"
     tRNA            283..355
                     /locus_tag="ON498_001133"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="ON498_001134"
     tRNA            363..444
                     /locus_tag="ON498_001134"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="ON498_001135"
     tRNA            462..534
                     /locus_tag="ON498_001135"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="ON498_001136"
     tRNA            546..617
                     /locus_tag="ON498_001136"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="ON498_001137"
     tRNA            634..719
                     /locus_tag="ON498_001137"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
     gene            730..803
                     /locus_tag="ON498_001138"
     tRNA            730..803
                     /locus_tag="ON498_001138"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Arg,seq:acg)
     gene            813..886
                     /locus_tag="ON498_001139"
     tRNA            813..886
                     /locus_tag="ON498_001139"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:tgg)
     gene            906..978
                     /locus_tag="ON498_001140"
     tRNA            906..978
                     /locus_tag="ON498_001140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ala,seq:tgc)
     gene            1006..1079
                     /locus_tag="ON498_001141"
     tRNA            1006..1079
                     /locus_tag="ON498_001141"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Met,seq:cat)
     gene            1093..1166
                     /locus_tag="ON498_001142"
     tRNA            1093..1166
                     /locus_tag="ON498_001142"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:cat)
     gene            1190..1279
                     /locus_tag="ON498_001143"
     tRNA            1190..1279
                     /locus_tag="ON498_001143"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:tga)
     gene            1292..1365
                     /locus_tag="ON498_001144"
     tRNA            1292..1365
                     /locus_tag="ON498_001144"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1384..1459
                     /locus_tag="ON498_001145"
     tRNA            1384..1459
                     /locus_tag="ON498_001145"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Asp,seq:gtc)
     gene            1461..1531
                     /locus_tag="ON498_001146"
     tRNA            1461..1531
                     /locus_tag="ON498_001146"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Gly,seq:tcc)
     gene            1545..1618
                     /locus_tag="ON498_001147"
     tRNA            1545..1618
                     /locus_tag="ON498_001147"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Ile,seq:gat)
     gene            1643..1730
                     /locus_tag="ON498_001148"
     tRNA            1643..1730
                     /locus_tag="ON498_001148"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Ser,seq:gct)
     gene            1752..1823
                     /locus_tag="ON498_001149"
     tRNA            1752..1823
                     /locus_tag="ON498_001149"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Glu,seq:ttc)
     gene            1846..1935
                     /locus_tag="ON498_001150"
     tRNA            1846..1935
                     /locus_tag="ON498_001150"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Ser,seq:tga)
     gene            1948..2021
                     /locus_tag="ON498_001151"
     tRNA            1948..2021
                     /locus_tag="ON498_001151"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1982..1984,aa:Met,seq:cat)
     gene            2040..2112
                     /locus_tag="ON498_001152"
     tRNA            2040..2112
                     /locus_tag="ON498_001152"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Asp,seq:gtc)
     gene            2116..2188
                     /locus_tag="ON498_001153"
     tRNA            2116..2188
                     /locus_tag="ON498_001153"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Phe,seq:gaa)
     gene            2193..2263
                     /locus_tag="ON498_001154"
     tRNA            2193..2263
                     /locus_tag="ON498_001154"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2225..2227,aa:Gly,seq:tcc)
     gene            2276..2349
                     /locus_tag="ON498_001155"
     tRNA            2276..2349
                     /locus_tag="ON498_001155"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Ile,seq:gat)
     gene            2361..2432
                     /locus_tag="ON498_001156"
     tRNA            2361..2432
                     /locus_tag="ON498_001156"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Glu,seq:ttc)
     regulatory      2621..2700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2698..3891
                     /locus_tag="ON498_001157"
     CDS             2698..3891
                     /locus_tag="ON498_001157"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3939421.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            4021..5493
                     /locus_tag="ON498_001158"
     CDS             4021..5493
                     /locus_tag="ON498_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939422.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5576..6364
                     /locus_tag="ON498_001159"
     CDS             5576..6364
                     /locus_tag="ON498_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR3939423.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6431..7099)
                     /locus_tag="ON498_001160"
     CDS             complement(6431..7099)
                     /locus_tag="ON498_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR3939424.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7214..8926
                     /locus_tag="ON498_001161"
     CDS             7214..8926
                     /locus_tag="ON498_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939425.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8929..10698
                     /locus_tag="ON498_001162"
     CDS             8929..10698
                     /locus_tag="ON498_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939426.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10903..11469)
                     /locus_tag="ON498_001163"
     CDS             complement(10903..11469)
                     /locus_tag="ON498_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3939427.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11469..12425)
                     /locus_tag="ON498_001164"
     CDS             complement(11469..12425)
                     /locus_tag="ON498_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3939428.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12425..13081)
                     /locus_tag="ON498_001165"
     CDS             complement(12425..13081)
                     /locus_tag="ON498_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3939429.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13451..15469
                     /locus_tag="ON498_001166"
     CDS             13451..15469
                     /locus_tag="ON498_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3939430.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15539..15745
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15896..18310
                     /locus_tag="ON498_001167"
     CDS             15896..18310
                     /locus_tag="ON498_001167"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3939431.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18658..20211
                     /locus_tag="ON498_001168"
     CDS             18658..20211
                     /locus_tag="ON498_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3939432.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20592..22376)
                     /locus_tag="ON498_001169"
     CDS             complement(20592..22376)
                     /locus_tag="ON498_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939433.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22391..24112)
                     /locus_tag="ON498_001170"
     CDS             complement(22391..24112)
                     /locus_tag="ON498_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939434.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24212..24973
                     /locus_tag="ON498_001171"
     CDS             24212..24973
                     /locus_tag="ON498_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3939435.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7864 a         4668 c         5412 g         7460 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt ggtagagcac
      841 ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat tataaattta
      901 aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
      961 cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg tgtagctcag
     1021 ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct ccgccgcgat
     1081 ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata accgtccggt
     1141 cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg gaggattacc
     1201 caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc gtgcaagggt
     1261 tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg gtagctcgtc
     1321 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa aactaaaaac
     1381 actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg
     1441 attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc cttccaagct
     1501 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct atagctcagc
     1561 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1621 agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc gcccctgcta
     1681 agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg tttcaatatt
     1741 ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac
     1801 gggttcgaat cccgtacggg tcattaattt aatatagttt tacatggagg attacccaag
     1861 tccggctgaa gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc aagggttcga
     1921 atcccttatc ctcctttttc atattatcgc gggatggagc agtctggtag ctcgtcgggc
     1981 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca attaaaaact aaaaacattg
     2041 gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg ggttcgattc
     2101 ccgtcggaac cgtttggctc ggtagctcag ttggtagagc aatggattga agctccatgt
     2161 gtcggcagtt cgattctgtc ccgcgccatt atgcgggtgt agtttagtgg taaaaccaca
     2221 gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gcttttagtt tttatgggcc
     2281 tatagctcag ctggttagag cgcacgcctg ataagcgtga ggtcgatggt tcgagtccat
     2341 ttaggcccat ttattttatt ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
     2401 cggtaacacg ggttcgaatc ccgtacgggt catggaagat actttttgag tatctttttc
     2461 tttttatctg aaagaatgat tttaaaaagc ttagcagagt tatctttatg gatgattgtg
     2521 ctaagctttt ttggacagtt ctaatgtaaa atatttttat attttttaag caaaaaaatg
     2581 aaaaaatacg agcagaaaac tgttttatct gctatacttg gttcgttgca agttcccgaa
     2641 aggatccgca ggacggagat gccttgtaac cgaatttaac tagggggaat cattataatg
     2701 gtagaaagac acttatttac atcagaatct gtttctgaag gacatccaga taaaattgct
     2761 gaccaaatca gtgatgcaat cttggatgca attttaaaac aagatccaac agcacgagta
     2821 gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat ttggagaaat atctacgacg
     2881 gcatatgtag atattcaaaa agtcgtacgg gaaacaataa aagaaattgg ttatacacgt
     2941 gcaaaattcg gatttgatgg agatacagca gctgtattgg ttgcgattga tgaacaatct
     3001 cctgatattg ctcaaggagt tgatgaagca cttgagatcc gggatgaaga taaaaaagac
     3061 gtattagatg agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc tgtagatgaa
     3121 acaccagaat tgatgccttt accaatcgct ttgagtcatc gtctggtacg acgcttggca
     3181 gatttgcgta aatcaaatga attaacgtat ttacgtccag atgcaaaatc tcaagtaacg
     3241 gttgaatatg atgatcaagg acaaccggaa cgcgtagata caatcgttat ttcaacacag
     3301 catgatgatg cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa agtagtgaag
     3361 gaagttattc cagctgaatt attagatgat caaacgaaat attatatcaa tccgactggc
     3421 cgatttgtca ttggtggtcc tcaaggggat gccggattaa caggaagaaa aatcattgtt
     3481 gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa agatgcgaca
     3541 aaagttgacc gttctgccag ctatgctgca cgttatattg ctaaaaacat tgttgcagca
     3601 gggcttgctc gcaaagcaga agtacagcta gcttatgcga ttggtgttgc tcaacctgtt
     3661 tcgatctcaa tcaatacttt cggtactgga acggttccgg aagaagaact gattgctgca
     3721 gtgagagaaa acttcgatct tcggccagca ggaatcatcg agatgcttga tttgcgtcgt
     3781 ccgatttaca aacaaacagc tgcttacggt cattttggtc gtacagatgt agatttgcct
     3841 tgggaacata cggacaaagt agatgcgttg aaagcaagtt tagcaaaata agttatattc
     3901 agctggaacg atcgccaagt ggtggttgtt ccagcttttt ttatggcagt taacctcttg
     3961 aaaaagatag taattattta tctattttta gatcctgtca taatgaaagg gaggaataag
     4021 atgaaaaaag aaacaaatgt tactttggtc acagtgagta ttttcattgc aacgttcatg
     4081 acggctattg aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg ttcattacat
     4141 ggaatggaaa tcatgaactg ggtattttcg atttatttgc tgacaaacgc tatgcttact
     4201 ccgatttacg gaaaacttgc ggataaagtg ggacggaaac caatctttat ttttgggaca
     4261 ttattgttta ttatcggttc gttattttgc ggtttatctc aaagcatgat tgggttgatt
     4321 gctgctcgtg cgattcaagg gatgggtgct ggggcaatga tgccagtagc tttgacgatc
     4381 atcggcgatc tttattctat tgaaaagcga gcaaaagttt taggtttgaa tagttcagcc
     4441 tgggggattg ccagtgtttt tggaccgctt gcgggaggat tcatcgttga aacgatcagc
     4501 tggcactgga tcttttttat caatgtgccg atcggaattc tattaattat tttgatttgg
     4561 ctatatttaa atgaaccaaa aatcatacat gagtctaaac caatggatat ttttggtagt
     4621 tttagtttga tggccatgct tgtctcattg ctgttagggt tccaattatt aggagacgaa
     4681 ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt aggtattttt
     4741 gtttttgccg aaaaaagagc taaagatcct gtcatcatga tgcatttatt caaaaatcca
     4801 ctatttgttg tggtcaactt ggctgcagca ttggctagtg gttttttgat gggaatcgat
     4861 gtttatatcc ctatgtggat gcaaggtgta ttagggctga atgcagcaat tggtgggctg
     4921 gtattagcac cattatcact agtctggatg gtcggttctt tccttgcaag caagtggatg
     4981 actcaaatga aaactcattg ggtcttgaga attggactac taatcgcagc tattgggggc
     5041 gcttggttgt atcttatgcc tttcagaagt aactatttat ggttttttgc tatatcaacg
     5101 gtcatcggta taggcttagg tgtgaccatg actacctcga ctgtcagtgc acaaaatagt
     5161 gtatctcgtg accagcttgg tgtagcaact tcttttaata cgttagttcg aacaattggt
     5221 caaacagtca tggtttctat ttttggttta ttgataaatg gggtaactaa acgagaactt
     5281 gcagcagcaa atttaacaaa tgatgcagat atcatgaata aactggtgaa tccacaaacg
     5341 gctaaatata ttgatgcgga tattttgtta ccattaagaa aaattcttta tgatgggtta
     5401 cattccgttt atctagttgg cttgttactc gtgatcattg ctttggtctt aacgcttttc
     5461 gttcatgaaa aaagtggtaa aataaaacag tagtttcggt taagatatgt acgtagccga
     5521 atcattcaaa tttaaagtta ttcaaaaata acttcttact aaggaaggcg aaaatatggc
     5581 atttattcaa gcaaatattt attcgaatgt gttagaaatg gaagtcaatc taaatgtgat
     5641 actaccgcaa acaacaaaaa aggtgattgg caccagttca gagcaagaga caacggatat
     5701 acctgtttta tatttacttc atggaatggg tggcaatcat agtgtatggg aaagacgtac
     5761 gtcgattgaa cgttatgtag caaactacgg attagctgtc atcatgccat ctactgatct
     5821 tggctggtac acagatacag tttatgacat gaagtattgg acattcattg cagaagagct
     5881 acctgagatt tgtcatgaat tattcccgca attaacaaga aaaagagaaa aaacatatgc
     5941 cgctggatta tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa aatcagaaaa
     6001 tttcgcagca gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa tggatgatct
     6061 tttgatggtt cgtaaagcat ctttttggga agggatcttt ggaccattgg atcaaatcga
     6121 aggttcaaaa aatgatccgc aatacttatt gaaagaacta gtaaaaagcg gaaaaccagt
     6181 tccaagattt tatatggctt gtggggaatc tgattttctt tatcaggcaa atcaggaaat
     6241 ggtatctgct atgcaggaac aaggcttggc ggtcaccttt gaacaaggac ctggcgatca
     6301 taactggatc ttctgggacc aatggattca acgtgcattg gcgtggctaa tggaagaaaa
     6361 ataaaaaaag agtcttaaaa agatagctcc tatagagatc acaaacatag tgatctctat
     6421 aggagctttt ttactgctcg ttttctcttc ttttattttg ttggcataag ccttcaatat
     6481 aggtttgtgc actcgttaga aagagtgctt tttcattcaa ttctgcatgg gactgcatac
     6541 tttctttaaa tccctctaat tgattttcaa ctacataatt acgcataaat agaactgttt
     6601 ctttcaaaaa ttcatttccg tccataattt tttgtcgaag ctgctgttca tgctgctgat
     6661 ccattaactg tccaatcaat aaattgtgga gagaggcaat ggcctgatga ctttgggtga
     6721 ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc caaatgttct aatcgactcg
     6781 gaaaagcggg cacagtattg atttctaatt ctgccccaca agggaaacgt gtataaaggt
     6841 catagtaatt ttccatatac tgcagcaact gttcattcca aggcaaagtt ggatctggaa
     6901 gcgctaattc ttcttccatt gtttcagcca ttatttgcaa gagctgttgt ttgtttttga
     6961 aataccaata taaagcaggt gcttgaacat gaattctgtt tgcgatgttt cgcatggaaa
     7021 gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg atgattgctt
     7081 gagaaagctt tatttccatt ttatcaacct cgtgtattgc attatctttt taaacatggt
     7141 aaattgttag tcggtctaat tttacagtgt taaattagtc ctgtcaattt aaacaaaggg
     7201 gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa aaatataaag tagctgtttt
     7261 tatttccttg ctgtccgtag cagtcatggt tgcagcagca ttatggcaac ctaagttact
     7321 acagcaagta ttggaagcta ttattacaga agataatgat gaaatgaaaa caattggtat
     7381 ttacctgatc agtctggctt tgctaggatt agccgcaggt gtaacgaaca cgattttctc
     7441 tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa gaagcatttc gaaagatcca
     7501 gacattttca tttggaaata tcgaacagtt ctctgctgga aatcttgttg ttcgtctaac
     7561 aaatgatatc acgcaaatcc aaaatttagt gatgatttct ttgcaatcac tttttcgaat
     7621 tccattctta tttattggtg ccttcatttt agctatgatc acgatgccgc aattatggtg
     7681 gatcatcgta gcgcttatcg ttacggtatt tctgattacg gcactttcat ttacaaggat
     7741 gggcaagcac ttcatgatca ttcaaaaact gattgatcgt gtgaatagca ttgcaaaaga
     7801 aaacttacta gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc aattgaagtc
     7861 attcagcaaa gtaagtgagg acttaacaaa acacaacatc atagtcggta cattattttc
     7921 tgtgatgatc ccgtccttca tgctggctgc caatttggct gtggttgggg cgatcttttt
     7981 tgtgagcgat cttgttaaag atgatcctac gttaattggt ggaattgctt cattcatgaa
     8041 ctacttgatg cagattatga tggcaatcat tattggcgga atgatgatga tgatgacttc
     8101 aagagcagcc gtatcgctga agcggattgc ggaaatttta gatgctgaac cagatcttac
     8161 ttatttagac gttccagaac aagagttgac gggttctgtc acttttgacc atgtttcttt
     8221 tcgttatcct ggtgaggata cagatacgct taaagatatc agcttttcta tcaaacctgg
     8281 agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag tcaacactag cacaattgat
     8341 ccctcgtcta tttgatccga cagaaggcaa agttgaagta ggtggtgtag atcttcgtca
     8401 agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag caattttatt
     8461 ctcaggaacg attgctcaaa acttgcgcca agggaaaaag aatgcaacag aaaaagagat
     8521 ggaacatgct tcttctattg ctcaagcaaa agaattcatc gaaaagttat ctgatcgtta
     8581 cgaagcacca atagaagaac gtagcagtaa cttttctggt ggtcaaaagc aacgtttatc
     8641 cattactcgt ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca gtacgagtgc
     8701 actggatgct cgctctgaaa agctggtccg agaagcactc gatcgagatt tgaagggaac
     8761 aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc gcatcttagt
     8821 attggatgaa ggcagattag tcggcgaagg aacccatgag gaattggtag ctggaaaccg
     8881 tgtctatcag gaaatctttg aaactcaaaa aggaacggag gagtaagtat gactgatcta
     8941 ataaaagcaa gtaaattttt ctatcactat ttgaagcggt acaaactctc ctttttcttt
     9001 atctttatca cagtgatcat tgctacctat ctacaagtaa aggcacctca atacatcggg
     9061 gaagcttttc aagaattagc taattacgta ggaggcttga tgcaaggcgt agacgataaa
     9121 agcaaatttg tttctgtaat atggaaactc ttgctgtttt atgtactagc tagtgcagcc
     9181 aactttatct atagtatttt gttcacacaa gtagtaggga agtcaacgaa ccgaatgcgt
     9241 atcggtctat tcaacaaatt ggaaaaattg acgatccgtt tctttgattc tcatcaagac
     9301 ggtgaaattt taagtcgttt taccagtgac ttagataata ttcaaaatag tttgaaccaa
     9361 gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat catgatgttc
     9421 cgccaaaatg tagagttagc ttgggctaca attgcttcca ctccagtagc cgttttgata
     9481 gctgtcttga ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga tgaagtgggg
     9541 aaactaaatg gttatatgga tgaaaaaatc agcggacaac gtgtcatcat cacaaatggc
     9601 ttacaagaag aaacgattga aggattctta gagcataatc aaagtgttcg cgaagcaacc
     9661 ttcaaaggac aagcttattc tggtctacta tttccgatga tgcaaggaat gtcactggtc
     9721 aatacagcga tagttatctt tttcggcggc tggttagcat taaatggtga tttggaacgt
     9781 tcggtggcat taggcttagt tgtgacattc gtccagtatt cacaacaata ttaccaacca
     9841 ctgatgcaga tttcatctgg atacagcatg atccaattag cgattacagg ggcaagacga
     9901 ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag aaaactgtaa agcatttgat
     9961 ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg gttattctcc agaaaaacta
    10021 gtcttgaaag atgtctcgat caatgtggac aaaggagaaa tggttgctct tgttggtcca
    10081 acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc gtttttacga tgtcaatgat
    10141 ggttcagtag tcatcgatgg aatagatatc cgagattatg atcttgatgc attacgtaga
    10201 catgtaggaa tcgttttgca agaatctgta ttgttctccg gaacgatcaa agacaatatc
    10261 acattcggca aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa acaagcgaat
    10321 attcatgact tcatcatgac gttagaaaaa ggttatgata cagagatatc cgaagaaaat
    10381 aacttattca gtacaggtca aaagcagttg atcagtattg ctcgaacgat catcacaaat
    10441 ccatctttat tgattttgga tgaagcaaca agtaatgtag acacggtaac agaagcaagg
    10501 atccaaaaag caatggatga agcaatcaaa ggaagaacca gctttgtgat tgctcaccgg
    10561 ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc gtgatgggga agtcattgaa
    10621 gaaggcagtc atcacgagtt attagcagaa aatggatttt atgcggaact ttatcataat
    10681 caatttgtat ttgaataaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    10741 gccaatcaaa aacagcttct catgtttttg atttggttgg acttattacc gcagatataa
    10801 gcttttgagc accgtgaata aagaggttat gaaatcaacg ctctaacctg agtattagag
    10861 aaaaaaacgc aaaacatatc atttatgttt tgcgtttttt atttatcttt ttttctcaat
    10921 acaatataaa ataggcggct ggttcttttg gttgataaac cgataattca gcacattata
    10981 tgtttcctgt ggcagtttgg aacaaaaaga atcaaccata tccagttctt tttctccacc
    11041 ttcatgacca tagtagacga caaggatcaa tcgtccgcga ggcacaagtc gtttcaatat
    11101 ttcttccata gctgtgcgtg tagtctctgg catagtgatg attgctttat cactttttgg
    11161 gagataccct agattgaaaa tccctgcttt gagattttgt tgttcagcta tcacttctcc
    11221 taacgtttcg tggccttgta gaaacaatga gacacgttct tctaactgac gctgttctag
    11281 cctttcacga gtagcatcga ttgcttgttg ctggatatca aaactataga catgaccagt
    11341 ttttccgatg tgttctgcta gaaaaaccgt atcgtggcca ttgcccatcg ttgcatctac
    11401 gacgtgatcc cctggttgca agatttcttg cagtaatgta tggctgaaat gcaaagcagt
    11461 ttgcaacatc agacgctcac ctttttccgt acatcatatt ttccttggaa gctatctctg
    11521 cgaagaagtt cttcatcaat tgcattcaag acttcccatt ttttcaagct ccacattggt
    11581 ccaatcaagg aatcccacgg cgcatcccct gtcaaacgat ggatgatgat ctcttttggt
    11641 atcatttcca gctggtcgca aatgacattg acatagtcag gacggctcat taattgaagg
    11701 cgaccttcat gatagtcccg caacattctc gttttgcgca tcagatgcat caaatggagc
    11761 ttgattcctt ggatatctga atctaaaatg gtgcgtctga cattttcttt catcatttct
    11821 aaactttcgc ctggtaatcc gttgatcaaa tgcgtacaaa cacggatatt atgtttgcgt
    11881 aatttagcca cgccatctaa ataagtttga taatcatgtg ctcggttgat cagttttgaa
    11941 gtttcttcaa aagttgtttg caatccaagt tctacccata aatacatccg ctcgttcagt
    12001 tcagcaaggt aatcaactac ttcatctggc agacaatccg gtcgtgtacc gactgataat
    12061 cctacgacac ctggtaaatt gactacttgc tcaaatcgtt ctttgattat ttcaactggc
    12121 gcatgggtgt ttgtaaaatt ttggaaataa acgatgtatt gttggacatg aggccatttt
    12181 tgatgcatca tgtcgatttc tttctggaat tggattggaa gagggtcttc aggtgcaacg
    12241 atcatgtctc ccgaaccaga tacactgcaa aaagtacaac cgccatgggc aacagtccca
    12301 tctcgattcg gacagtcaaa tcctccatcg atcggcacct tgaaaatttt cccgccaaac
    12361 tcatttcgta aagcgtaatt ccaagaatga tatcttttat ttccttctga ataaggaaaa
    12421 gtcattaaaa gtccttcttt ctgtaagagt tatctatttg ttttcttttt tctctataca
    12481 ctggataagt caggcacagc caagaaagac tgagtaagta accgccgatg atatcagtag
    12541 gaaaatgaac gcccagataa atgcggctta tcccgacact tactataata aacatcatca
    12601 gcaattgaag caatgtgcgt aaggttcttt tttgaacaaa agctggaagc agaaatagta
    12661 aagaaccgta aagaatcata ctgcctgctg catgtccact agggaaactg taacttgttt
    12721 caactactaa gtgcgccaat gaaggacgct ctcgcatgaa aaatagtttg atcaccgggt
    12781 tgatgattcc agcaattaca ataaatccag aaacgaacca aaatgtttct gccctttttt
    12841 tcttatacca catccataaa gccgcacaag caaataaaat gatgacggta agaggattac
    12901 cgaatttagt cataaagagt tggtagctgt tcagatcagg ataagaagct ctgacaatgc
    12961 ctgtaaacca ctgatcgaaa ccagaaagcc acgacagatg gaactttacg atccagccca
    13021 aaatgaaaaa caggacaaga aagaagctac cagccacctg aaatgatgtt ttatatttca
    13081 tacaattcct cctttgaagc aatgacttat tatagcattt ttggtccagc gaaaaaaacg
    13141 gtaaagagat aaaaccaatg tatgattcct ctttgaataa ccaatattct tataaaaaaa
    13201 ataactgtaa tcggaaagac attttttggt aatgcaaatg aaatgttagg gaaataaaat
    13261 cagtgttttt cgaaatcctt ttaaacaaga acgtagacgt aggaagaact tgaaaatgaa
    13321 cagaagttgt tcattacggt aaattacaat atgattacaa aaaacaacaa agctcttaaa
    13381 taacttttaa taaatattaa gagaagttat aattattaat aatagataaa ttgaagttgg
    13441 aggatagaag atggaaaaca ttgcaagaaa agaacggaga cgtttagaac agaccaaacg
    13501 ttttcgaaaa gcaaaacgag gggcagcaat cgtgggaact gcgatggtag gctgttctgt
    13561 cgctgcccca ttagtacagc cggttcctgt tcaagcggat gaaacacctg ttcagttcgg
    13621 cgcaagaatc aatacagcgg catttatcac agaaattgct acttatgctc aaccaatcgc
    13681 tcaagccaac gatctatatg catcagtgat gatcgcacaa gcagttgtag aaagcggttg
    13741 gggcagtagt gcgttatctc aagcacctta ttataatctt tttgggataa aaggcagcta
    13801 tcaaggacaa accgtctaca tggatacgct tgaattttta aataatcaat gggtaaataa
    13861 aaaggagcct ttccgacaat atccttcttt tgctgaatct ttcaatgaca atgcctatgt
    13921 acttagaaat acttcatttg gcaacggtta ttactatgcc ggtacttgga agagcaacac
    13981 gaaatcctat acagatgcga cagcttgttt gacagggcgt tatgcgactg atcctggtta
    14041 cgcaggcaaa ttaaataata tcatcgcaac ttacggattg acccaatatg atacaccagc
    14101 tagcggaaac gcaggcggtg gagcgactac aggtggcaat aatactggaa gtgcaggtag
    14161 tactggcggt tcaagcaatt ctggtaatac aaactcaggt tcagcaactt cgaccactta
    14221 tactgtccaa tctggtgatt ctgtttgggg aatctcaaat aaattcggta tcacaatgga
    14281 tcaattgatc caatggaaca acatccaaaa caatttcatt tatccaggac aaaaactgac
    14341 aatcaaaggt agccaggaaa atggctcttc gacgaataat tccggtaaca ataccaattc
    14401 ttcaggaaat gctggtacat caaacggagg acaaacaact ggtgcgaaat acactgttca
    14461 atctggtgat tctgtttgga agatctcaaa tgatcacgga attacgatgg ctcaattgat
    14521 tgaatggaac aatatcaaga acaatttcgt ttatccagga caacagctaa tcgtatcaaa
    14581 tggcggttct tctaatgctg gagcaaatac tggctcaaat actgggaata caagtggctc
    14641 tagttcgtcc agctcaagtt cttctaatac agcttcagga acgaaatata cagttaaagc
    14701 cggagactct gtttggagcg tctcaaataa atacggaatt acgatggctc agctaatcca
    14761 atggaacaat atccaaaata actttatcta cccaggacaa caactgactg tttcaaacag
    14821 tggatcaaca aacagcacgt caggtaatac aggttcgtca accaatacaa ataacagcag
    14881 ttcagttaat actggcagca gttcgtctca aggcacttat accgtgaaag caggtgaatc
    14941 tgtttggagc gtggcaaata aaaacggcat cacgatggat cagttaatcg aatggaataa
    15001 tatcaaaaac aacttcattt atccaggaca gcaattgatc gttaaaggtg gaacttcggc
    15061 aaatacaaat acaggttcaa ctacttctgc agcgaaacca aatacaccga atacgtctgc
    15121 tacgacaagt acatcatcca ctggtaatac gatgtatacc gtgaaagcgg gcgagtcagt
    15181 ttggagcgta gcgaacaaac atcatatcac gatggatcag ttaatcgaat ggaataatat
    15241 caaaaataat ttcatctatc caggacagca agtgattgtg aagaaaggtt catctcaatc
    15301 aacaaaccaa caatcagtta ctggttcgaa aacctatact gtgaaatctg gagaatctgt
    15361 ttggagtgtt gctgacagtc atggaattac aatggctcaa ctaatcgaat ggaacaatat
    15421 taagaacaac tttatttatc caggtcaaac gttgattgta gctaaataaa atagctgttc
    15481 gaagaaataa cgattgaatt ccacaaaaat atttgttact ataaggctac ttaaatatga
    15541 caacacttga aaaggaagta gtaaaaagac tgaagtttca gagagcgcat ggctggtgag
    15601 aatgtgcacg gatgcttttg aactcgcctt tgagttgccc ttgctgaata cccgagtaag
    15661 caagcggcgg tgacggccgt tacaacgttc gaggcctttt cactaaggta aaattaggtg
    15721 gtaccgcgta gattacgtcc tataaaaaga gcaatctttt tataggattt ttttatttct
    15781 tttgtgaagt ttgagaaaac aaagcaaaag gaaataaaaa taaacagatt ctgccggttt
    15841 tttcgttata tttataatag aaacaagtgt tttcaataga acaggaggac agcaaatgag
    15901 ttacaatcat aaagaaattg agaaaaaatg gcaaaaatac tgggcaaaaa cgaatacttt
    15961 caacacccat gatgatcctg aaaagccaaa attttacgca ctggatatgt ttccctatcc
    16021 atccggtcaa gggctacatg tagggcatcc tgaaggttat acggcaacgg atatcctttc
    16081 acgtgtgaaa agaagtcaag gatacaacgt gctgcatcca atggggtggg atgcattcgg
    16141 tctgcctgct gaacaatatg cgctagatac aggaaacgat ccagctgagt ttacccaaaa
    16201 aaatatcgaa acattccgac gccaaatcaa ttctttagga tttagttatg actggaatcg
    16261 cgaagtgaat acaactgatc cggagtatta taaatggaca caatggatct ttacgaagct
    16321 ttatgaaaaa ggtctagctt atgaagcaga agtagcggta aactgggtac ctgaattagg
    16381 aacagttatt tcaaatgaag aagtgatcga tgggaaaagt gaacgtggtg gctatgatgt
    16441 agtcagaaaa ccaatgcgcc aatggatgct gaaaatcact gcttatgctg atcgcttatt
    16501 ggatgatcta gaattagttg attggccaga aagcattaaa gaaatgcaaa gaaattggat
    16561 cggtcgttca gtaggagcaa atattgaatt taaagtggct ggtacggata aatcctatac
    16621 cgtatttacg acacgaccag atactctatt cggtgcaact tactcggttc ttgcacctga
    16681 actagatctt gttagagaaa ttacgacccc tgaacaaaaa gctgctgtag aagcttatat
    16741 cgaagagaca gctaaaaaat cagatctaaa gagaacggat cttgcaaaag aaaaaacagg
    16801 agtatttact ggtgcttatg caatcaatcc agttaatggc aaggaaatcc caatctggat
    16861 cgccgattat gtattatctt catacggcac aggagccatc atggctgttc cagcacatga
    16921 tgaacgtgac tttgaattcg ctcaaacttt cggactagaa attcttccag ttatcgaagg
    16981 tggagatgtt gaaaaagctg cttacacaga agatggtacg cacatcaact ctgaatttct
    17041 agacggcatg aacaaacaag aagcaatcga taaaatgaat gaatggctag aagaaaatgg
    17101 cgtagggaaa aaagaagtca gctatcgctt acgtgactgg ctattctccc gtcaacgtta
    17161 ctggggtgaa ccaatcccaa tcatccattg ggaggacgga acagtcacag ctgtcccaga
    17221 agaagagcta ccattacggt tgcctaagac aaaaaacatc aagccaagcg gtactggaga
    17281 atctccatta gcaaatatcg aggagtgggt caatgtcgtt gatcctgtaa caggtaaaaa
    17341 aggccgtcgc gaaacgaata caatgccaca atgggcagga agctcatggt attacctgcg
    17401 ttatatcgac cctcataaca aaaaagaact agcagattat gaaaagttga agcgttggct
    17461 accagttgac atctacattg gtggagcaga acatgcggta cttcatttac tatatgcacg
    17521 attctggcat aaattcttat acgatatcgg tgttgttcct acaaaagaac cattccaaaa
    17581 actatacaat caaggaatga tcttaggcga aaacaacgaa aaaatgtcaa aatctcgtgg
    17641 taatgtagtc aatcctgatg atgtggtcga aacttacggt gcagatacac tgcgcatgta
    17701 cgaaatgttc atggggccat tagatgcttc gattgcttgg agcgaaaatg gcttagaagg
    17761 aagccgtaaa ttcttggatc gtgtatggcg tttgatcgtt gatgagaaca ataaaatgcg
    17821 tgaccgtatc actacattga atgatggcaa gcttgataaa gtgtatcatc aaacagtgaa
    17881 aaaagtgaca gaagactatg aaaatcttca tttcaataca gcaatctccc aattgatggt
    17941 ctttatcaat gaagcctata aagtggacgc tctgccttat gaatatatcg aaggatttgt
    18001 tcaactactc gcaccgattg ctcctcacat gggtgaagaa ctatgggcaa tccttggaaa
    18061 tgatggtggt atttcttacg caccatggcc aacatacgat gaagcagcac ttgttgaaga
    18121 cgaagtagaa gttgttttcc aagtaaacgg taaagttcgt gcgaaaagca atgttccccg
    18181 agacttagga aaagatgagc tagaaaaagc agcattagcc aatgaaatag tgcaagaata
    18241 tattgaaggc aaaacagtca gaaaagttat tgcggtacca aacaagttag tcaatatcgt
    18301 tgctaattaa ttggctcttt gtcaataagg actgatgagt tgtgcaaaat caaatctggg
    18361 ccagaatgaa cttcattctg tctcagattt tttgttattt caaattgatt aattgattga
    18421 tcaaaaaggt tttgattttc atgttctcaa acacgttcaa tgtctttttc ttctgaatgg
    18481 tcttgtaaga gatctctgta atcatcaaat ttttgtctat taatcttagt attttttgat
    18541 gtataaacta cctgaaattg agagtacata ccaaattcat aaatttttta ttagtgggac
    18601 tagtgattgt atggattttg acgcataatg aatgaagtat gagaaaagga gagacaaatg
    18661 gatcaagaac tatttattat cgaagtatta tcagctaaac aaaaaattat cgagaaagcg
    18721 aaaaaaagag cgaaaagaat aatgttcaag cttttaagct tagtattttt ctctggtttg
    18781 ctttttttcg gagctgtatc gttcctatat cgccatatga gtgcagtagt ccgttcagct
    18841 gaagggatgc aaaaattaaa taatttgtat cacttttcgt ggggagttgg cggaataaca
    18901 attgttgtgc ttattgtttt tcttctactt atttggctcc gttaccatca attgtcgcgt
    18961 catttcgatc aatggcttag tcaaagagca caacagtttt tacataataa accaacttct
    19021 caagatgatg actatcttta ttttgagaag acttcgacga gtcaagcttt tcggatacaa
    19081 aaaagtgatt gccagttatt gctgagatca cttgatgatt tacctatcta cttaggcgaa
    19141 agcaaatcaa aattcggaat gaacacattc catatttttt tactagaaaa cgaacccaga
    19201 tgggaaaaaa gccagtcttc gcctggtgga tacctgacaa ctaagatgag tacattagtc
    19261 actggttttt tcctcattct tttagctgga acaatctatt atgggagata cactcagcca
    19321 aaaatgtctt ctcatataaa tgctgcttct cacatgacaa tgaactcaac taacgatacg
    19381 catatacaaa agacaaatga agggttgtta gctgatccta agcaaaaggt aaatttgaca
    19441 acacaaaaga taaatgatct caaacttgat acagagacgc actctctcta tatgacaaca
    19501 gatagtggtg ctaattgggt atttgtgccg attgattcgc aatggttgcg ttttggcgaa
    19561 tacaccctga caacggggct gattcctgaa ggttattgga tggacaacac atatgatatt
    19621 tccgccgatt tctcatggtt tatctattct tctggggatg atgtacgtct gctgacttca
    19681 agagataatg gtaaaacatg gcaaacgtct attatcaccc aatcagttca gccgatacgt
    19741 tatcgaaaag cggtatttta tggcaacgat ggtctattta tctgtacatc agccagtggg
    19801 atgtctgcag aaactttgta catttacact actatggatg gcggaatcac ttggataaaa
    19861 agtggaagta caatgatcaa tcagccagta caaaatgtca gcttcgttac aacttcttta
    19921 ggttttgttt cgacacgtga aagtttgttt tatacaaaca acggaggacg ctcattcaaa
    19981 gaatcagttc tgtcgattcc ggaaaactat cagacagggg ggatagatct ttttcaatca
    20041 ccaaatgaag ttactcaaat aagcgcgaat acgttagaga caaaatttta tttgttgaaa
    20101 aaaggaccaa tcgatgcagg gaaaatgttt gcctgcctct tccgttcaac tgataacggg
    20161 gaaacatggc aattcgttca gcaactatcg caagtagaac aaggagatta aagaagagag
    20221 attgtgagcc tgacgttcag caccgagtca caagaaacaa attcaaaaaa cagcttctca
    20281 tatttgttga atagtgtaac tcgtgattcg gagttgacaa gagcaacaag aagcgatttt
    20341 gaagtatagc ttctcgtatt ttacaagatc gtgtcttatt gccgataggt tgtctcttga
    20401 acaccgttta ttcgtaaaaa gggattgtga caagtctttt gtcactactt accgaacgaa
    20461 actgaaggag ctatccaaaa agtatcatta gtattattct ttgtgtttct agtggcacca
    20521 ctagtacatt taaaaaagct atcgatcatg gactaatcat acatgatcga tagcttttta
    20581 gataatgttt gttacgctat ctgaaattga ctattataaa gactggcata aagcgttggt
    20641 gattggagta gttcagaatg tgttcctttt tcgacgatat cgccattctt cattacaagg
    20701 atcatgtctg cattttcaat cgttgacaaa cggtgtgcaa tcacaaaact tgtcctatct
    20761 tttgtcacag cttccattgc ttgctgaatc aatgcttctg ttcgtgtatc tacgctagaa
    20821 gttgcttcat ctaaaataac gacaggagga ttcgctagaa tgactcgagc aattgtcaat
    20881 aattgttgct gtccttgaga taacgtgccg ttttcgctag aaatgatcgt atcatatccg
    20941 tctggcagtg ttcgaataaa gtgatcacat tgtgcgattt cagccgcttc gataatttgt
    21001 tctcttgtgg catccatttt cccgtaggcg atattatcag ctaccgttcc ttcaaacagc
    21061 caggtatttt gtaaaaccat cccaaaagaa cgacgtagtt ctccacgtga aagcttggta
    21121 atatcaagac cgtcgaattc gatctttcct ttattcagtt cataaaaacg catcagcaga
    21181 ttgactaaag tcgtttttcc tgcaccagtt ggaccaacga tcgctaccgt ttgttttggc
    21241 tttactgaaa aatcgacttg attcatcaat attttatctg atgtgtaacc aaactgtaca
    21301 tttttgaaag ctatttttcc tttcggttgt tctaaaagtc ttgcatctgg aaggtctgga
    21361 atttcatctg tttcatctaa tatctcaaat atcctatcga ttgctgccat tgcggattgg
    21421 atcgagttga tcacataaga agcggtagaa attggttcag aaatctgatt gatgtactga
    21481 agataagctt gtaagaagcc gatcgtcaca ccacctgata gaacaagcat agcaccgaat
    21541 accgcactta tgatgaaggc gagttgatta atgaaacgaa tggccggata gatagcaaag
    21601 ttcataaatt gagcacgttt aaaagcgttg tattgctttt tatttacttc gtgtatagca
    21661 gcttctgcat gagcttgctg attgaatgtt ttgaccacta gattaccagc taagatttct
    21721 tccattcgat tatttaaaac acccagttca tactgattct gttcagaaat ctgtttgttt
    21781 ttgttcgcaa tcttagttgt aacaattgaa ctgccaacga tcagcaataa gacgagcaaa
    21841 gtaagcttgc tttcgatata aaacagcatc agaccagcag cagctattgt tacgacagaa
    21901 gtaaaaaatt gattgatacc tgtcaataac acttgagaaa gttgattgag accggtagtt
    21961 gtccgactaa tgatgtcacc tacttgatga ttgtcaaaaa acgacatcgg taatgttttg
    22021 aatttttttg tgatttcttt tcgcaccctc aatgtgatct tttcgcttaa cgctgccatt
    22081 gtccgttcct gaataaatga agtgatgctg cttaagactg aacagccgat cagcaataag
    22141 acagggaaca acaatgcttg tttgatatca ctgaaagaaa gctgtccaat acctgttttt
    22201 cttatttctg ataagagatc atcgatcccg attcccatga tcaagggcat ggctacgact
    22261 aataggttcc cgaataaact gcatagaaac aatccgtaaa aatatttatg ttcaggtttg
    22321 atcattttcc aaaaacgtat caaagcacgc gtgttcaacg tttttttctt ttccatcgta
    22381 tcccttcttt ctatattcct tgtgattttg caaactcctg ataagtttgg caattagaca
    22441 gtagttcttc atgagtacct tgtccaacga tttttccttc atctaaaaca atgatgttat
    22501 ctgcttgttg gattgtactg aggcgttgag caacaatcac gagagttttc tccgacatgt
    22561 gtttatgaag ggctgctctt aatgctgcat ctgttttata gtctaaagcg gagaaactat
    22621 catcgaatac atagatctct gctggtttga tcaacgctcg agcaatacat atccgctgtt
    22681 tctggccccc tgaataatta gaaccgcctt gagcaacaaa gctgtccaat ccgtcaggaa
    22741 gggtgtccac tagttcttct aattgagcga tcttgatcgc tttagacaac tcttctgttg
    22801 aggcatattc atttcccatc tgcaagttgc ttaaaattgt cccgctgaat aagaaagcct
    22861 tctgagggac ataactgatg tgggagcgga tctgttcttg agtcatttga gtaagaggaa
    22921 taccaccaag ggttactttt cctatcgtag ggtcactaag tctcaagaga agtttcgcca
    22981 aactactttt tccagatccg gtcccgccaa cgatcgctgt tgtttttcct ttaggtattg
    23041 tgaaatcaat atcttctaga actggatcag agatttcagt atatttaaat gtgacatgtt
    23101 caaaagcagc tgcatttgtc tgatcaaagg ttggttcagc tactgttttg atcgtctctt
    23161 cctcaatttc gatctctgct tgcagtattt cttctatccg tcgtaatgag gcaaacgatt
    23221 tagggataat gaccaatacc atagctgcga tcatcaagta agcaagtgtc agcatagagt
    23281 attcgactac agcagtcacg acaccgattt gtaatgctcc ttctgctaca agatttccgc
    23341 caaaccacaa aatagaagca taaacgattc ccatgagcaa aaaagcaatt ggagtgaccc
    23401 aagcaaatat tcggttcact ttgatcattt gattagcata gtcagcaaag ctcttattcg
    23461 ttcgcttttc ttcatatgat tgattatcaa aggcacggat catattaatc ccagtaaaga
    23521 attctctcaa tgtgatggtg atccgatcca tttttggctg gatactcaaa gataaaggtg
    23581 cccctcgttt catcagtcga taaacaatcc atacataaaa aacaacagag atcaatgtaa
    23641 cccatgttag taatagagaa taacgaaaca tcatgagaat ggtgaaaacg caaataaccg
    23701 gtgctggtaa gatcatttgg caaaataaaa caatcatttg ctggacatta tcgacatcat
    23761 tcgtcattct tgtcaaaaga gatccagaac taaaacgact gatatccttc attgacagct
    23821 gttggaattt acggaaatat ttctcccgcg tttggaatcc aaacctagcg gaagccaatg
    23881 ctgaaaagta actagaggca actgcagcga ttgttcctag aaacgctaca agcagcattt
    23941 caactccaat tttttttata actgaggctt cgccacttgc tatacctgtg tcgatcagct
    24001 ttgctactaa ttttggtaca cctaatgtac cggcgatttg taaacagatc atcacaaagg
    24061 taaggagagc gagtcctttg tttttctgta agaatgtttt taagatagac atattattcc
    24121 tccttttttc ctgatacttt tcgattctat accttcaagt aacttgaagg tcaatagaaa
    24181 aaagaataaa ctgaaaaaga ggtgagaaaa aatgaaaaaa atgtatacga ttggccaagt
    24241 atcggaacta tttgatttgc caaaatcaac gatccgctat tgggatgaac aaggattaat
    24301 ccgttcaagt agacaagaag agaatgatta tcgattgttt gatatcgatg atatttttat
    24361 gctttatgat atcgtttttt atagaaaatt agatataccg atcaaacaga tgaagaattt
    24421 atatggaaaa acattgacgg agttatatga gacacttgat gaaacagaaa gaagaatcca
    24481 taaagaactg gtggtgatga aacaaaaaca aaaagagata agggagcaaa aaaaacagtt
    24541 gaaattgatg atcgatacaa acgaggaaga atttcctgta gaagagatcc cttttgactg
    24601 tatgatctca acggagtttg aagatattgt ggaaatcaaa aaatttctac ctaactattc
    24661 aagctttggg atgatgagta tgccagcttc cagttcacag acgatgtatg gattttttat
    24721 cgaccctagt gaagtacatt tatttactca agacgtgatt tgggagaaaa aagatacggc
    24781 tgtttatcgt cgattcttat tgaaatccga gatgaaccat gcggaacgta ataatatttt
    24841 agaaatcaga gaaagaatgt atgaaaaagg atggaaaaca ggagaagtga tcggccagta
    24901 tctattgacg aatacggatg aaaacaatat cagaactgaa tattatcatg catggataga
    24961 aatgaaaaaa taggaaatat gtgtttaaga taactcgaaa atgatggttg ttttagttat
    25021 aaagaaaaat tttctggttc gattacgggt ttaatgtggg ggattattag tcgtggatag
    25081 atagaatttt agttttttag aggaaatatg tctttaaaaa tggaatggtt gcaattctta
    25141 gcgatatata cgatgtaatt gtcctcttaa aaaagtatgg aaaaataatt gtattgggtg
    25201 ttatgtataa ttcatggtga actgagacta atgagaagaa aaattcatat tgttatgagt
    25261 gagattgtat cagatgagaa tgataaatta aataggtgca taaaagcatt acacttaaaa
    25321 tacattgttg cttacattat tacaatatgt taataaggca aaaataaata gatggattga
    25381 ataaacatat tttttaatca aata
//
LOCUS       DAJSVD010000032        24817 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSVD010000032 DAJSVD010000000
VERSION     DAJSVD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.032"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            260..808
                     /gene="aac(6')"
                     /locus_tag="ON498_001172"
     CDS             260..808
                     /gene="aac(6')"
                     /locus_tag="ON498_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3939436.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1354..3015
                     /locus_tag="ON498_001173"
     CDS             1354..3015
                     /locus_tag="ON498_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3939437.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(3085..3432)
                     /locus_tag="ON498_001174"
     CDS             complement(3085..3432)
                     /locus_tag="ON498_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR3939438.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3516..4457
                     /locus_tag="ON498_001175"
     CDS             3516..4457
                     /locus_tag="ON498_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939439.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4458..5501
                     /locus_tag="ON498_001176"
     CDS             4458..5501
                     /locus_tag="ON498_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939440.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5507..6577
                     /locus_tag="ON498_001177"
     CDS             5507..6577
                     /locus_tag="ON498_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939441.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6567..7520
                     /locus_tag="ON498_001178"
     CDS             6567..7520
                     /locus_tag="ON498_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3939442.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7532..8542
                     /locus_tag="ON498_001179"
     CDS             7532..8542
                     /locus_tag="ON498_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3939443.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8827..9648)
                     /locus_tag="ON498_001180"
     CDS             complement(8827..9648)
                     /locus_tag="ON498_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3939444.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9745..10023)
                     /locus_tag="ON498_001181"
     CDS             complement(9745..10023)
                     /locus_tag="ON498_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939445.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10291..12300
                     /gene="metG"
                     /locus_tag="ON498_001182"
     CDS             10291..12300
                     /gene="metG"
                     /locus_tag="ON498_001182"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3939446.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12779..13366)
                     /locus_tag="ON498_001183"
     CDS             complement(12779..13366)
                     /locus_tag="ON498_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939447.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13541..14863
                     /locus_tag="ON498_001184"
     CDS             13541..14863
                     /locus_tag="ON498_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3939448.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            15018..15794
                     /locus_tag="ON498_001185"
     CDS             15018..15794
                     /locus_tag="ON498_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3939449.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15787..16356
                     /gene="rnmV"
                     /locus_tag="ON498_001186"
     CDS             15787..16356
                     /gene="rnmV"
                     /locus_tag="ON498_001186"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR3939450.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16349..17233
                     /gene="rsmA"
                     /locus_tag="ON498_001187"
     CDS             16349..17233
                     /gene="rsmA"
                     /locus_tag="ON498_001187"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3939451.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17684..18313
                     /locus_tag="ON498_001188"
     CDS             17684..18313
                     /locus_tag="ON498_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939452.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18437..19546)
                     /gene="ugpC"
                     /locus_tag="ON498_001189"
     CDS             complement(18437..19546)
                     /gene="ugpC"
                     /locus_tag="ON498_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR3939453.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19755..20177
                     /gene="mgsA"
                     /locus_tag="ON498_001190"
     CDS             19755..20177
                     /gene="mgsA"
                     /locus_tag="ON498_001190"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3939454.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            20500..21036
                     /locus_tag="ON498_001191"
     CDS             20500..21036
                     /locus_tag="ON498_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3939455.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(21076..22839)
                     /locus_tag="ON498_001192"
     CDS             complement(21076..22839)
                     /locus_tag="ON498_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939456.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22842..24557)
                     /locus_tag="ON498_001193"
     CDS             complement(22842..24557)
                     /locus_tag="ON498_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939457.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         8038 a         4492 c         5029 g         7258 t
ORIGIN      
        1 tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac
       61 agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc
      121 tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg
      181 tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca
      241 agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa
      301 agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc
      361 agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga
      421 tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca
      481 tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta
      541 cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt
      601 agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc
      661 ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat
      721 cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac
      781 gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt
      841 caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta
      901 tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt
      961 ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct
     1021 taataccgag aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact
     1081 tttgtcacag tttctttttg tttattcttt tttatctttg tatggcaata atagacaata
     1141 taacgaaaat tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt
     1201 ttctgaaaaa ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa
     1261 aagtgttgat tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat
     1321 tattctgaaa attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg
     1381 gttgcggtat gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt
     1441 agttcagcaa aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa
     1501 gaaatgccta cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat
     1561 aacgtttatg aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct
     1621 agcgagttgg cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac
     1681 gcaaaatggt caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga
     1741 acagtagacc cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca
     1801 gaagacatta cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac
     1861 tatgaattag aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc
     1921 ccatcatttt tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct
     1981 gcaagtgata aagctgttta taatggacca ttcacattag aagattttga tggaccagga
     2041 acagatacag aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa
     2101 ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa
     2161 gatggccaag cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat
     2221 ccagcttata cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa
     2281 aagaattcac cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt
     2341 gaagctttag taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca
     2401 tcagatatgg taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta
     2461 gtttcttatg acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc
     2521 gttgattctt tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc
     2581 gaatatgttc aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct
     2641 gttccattct ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc
     2701 ggatgggctg ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat
     2761 tcatacaacc gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca
     2821 tcaacagacg ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc
     2881 atctctgatg atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat
     2941 gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca
     3001 tacgttgaag aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg
     3061 tcgcaacctc gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga
     3121 aagagcaatt agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga
     3181 cgagagcgag gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga
     3241 tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag
     3301 aaacggcaat gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt
     3361 gatgagattt tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga
     3421 tttttttttc atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac
     3481 atctgtcaga agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat
     3541 taaaacgtgt ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct
     3601 tgatgcaatt gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa
     3661 tcgagatgct aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt
     3721 atctttctaa tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag
     3781 tcgcacattt gttagctgga agaatcggac catccttgca gttagggtta caggcaatta
     3841 tctttggaac agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat
     3901 gggcagatac atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat
     3961 ttgctgtttt attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat
     4021 gggatggctt tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg
     4081 actctgctcg gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat
     4141 tagcaaaagc aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata
     4201 gtttgattcc attgatgact ttattaggac cgttagctgt agcattgatg accggatcac
     4261 tggtggtaga aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca
     4321 caacaaatga ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt
     4381 ttgttatttt gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag
     4441 aagggagccg aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc
     4501 ctgcggatga atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg
     4561 caccttcttt gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg
     4621 ttatttcaat ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt
     4681 ctcctcatga tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg
     4741 gaatcaatat cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat
     4801 atgctcaagc aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac
     4861 gtgatgtatt aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg
     4921 ctgcattact agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg
     4981 gacgtgtcga taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg
     5041 ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta
     5101 tggccatcac aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa
     5161 aagatcaaga atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat
     5221 tcaaacatat tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc
     5281 catctgctat tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt
     5341 cagcttcatt aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc
     5401 tattatggat tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg
     5461 atggattgcg tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt
     5521 agaagtgaaa gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg
     5581 cggtgtcaat tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag
     5641 tggaaaatca gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga
     5701 tgctggtcaa atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca
     5761 aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc
     5821 gacgatgccc attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa
     5881 aaaagaggga caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga
     5941 gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat
     6001 cgcgattgca ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct
     6061 tgatgtgact attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga
     6121 tacatcaatt atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt
     6181 cgctgtcatg tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa
     6241 cccgcaacat ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa
     6301 cgatcgattg tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga
     6361 cgcattctat ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt
     6421 ttttgaacta tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa
     6481 agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca
     6541 tacttacggt caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga
     6601 aacagtattt caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc
     6661 acatttatga aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta
     6721 ctggccgtac gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc
     6781 aagatataac gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga
     6841 tgatctttca agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg
     6901 ccgaaggaat tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg
     6961 atgatctatt gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat
     7021 tttcaggtgg gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt
     7081 ttatcatttg tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca
     7141 atttgttgca agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt
     7201 tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag
     7261 agatgggaac aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt
     7321 tatctgctat tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc
     7381 aacctgaacc agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg
     7441 tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat
     7501 ctcttctggt tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta
     7561 tccgacagaa gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta
     7621 taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc
     7681 gctcacaccc gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc
     7741 tttagcaaat gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc
     7801 cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca
     7861 aacccgtacg atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat
     7921 caagataaat cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg
     7981 atataatgaa gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc
     8041 tttagctaaa tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc
     8101 tctagaagta actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg
     8161 gaaattgaat gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag
     8221 caattatcag aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg
     8281 agaagattat cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt
     8341 agaatctttt ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt
     8401 aataacaaaa ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac
     8461 cgacggagca gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag
     8521 acattcggtt atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct
     8581 tgttaataaa aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc
     8641 actaattatg acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct
     8701 attgttaatt ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt
     8761 aactcacaaa gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc
     8821 acaaccttat tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc
     8881 tacaggacgt tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg
     8941 atcgatcgtt ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat
     9001 cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac
     9061 acggttgaat gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga
     9121 agactctcca gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt
     9181 gttactatga cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag
     9241 cggttcttct ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg
     9301 attaggcgta ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt
     9361 ttcttccttt gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc
     9421 accaggcaat gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt
     9481 cagctgatct tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt
     9541 gtcatattct ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt
     9601 atgttcataa tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc
     9661 attgacagta aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt
     9721 tacttgaaaa atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa
     9781 taaaaagaaa agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa
     9841 gccacaaata acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa
     9901 actgttcaaa tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa
     9961 tattaaatgt gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg
    10021 catgattgtt ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga
    10081 gaaataaaaa gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac
    10141 ctattcatcc ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt
    10201 cattttgaaa aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt
    10261 cgcatcatgc gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact
    10321 acgccaatct attatccaag cggcaaactt catatcggca actcgtatac tacgattgca
    10381 tgtgacgcaa ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga
    10441 gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa
    10501 gaatatgtag ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt
    10561 tatgataaat tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt
    10621 gatcgtttat tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt
    10681 tctgacgaag aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt
    10741 aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca
    10801 tacttcttcc gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc
    10861 gaattcatcc agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga
    10921 ttagaggatc ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat
    10981 ccgaaacatg tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga
    11041 tatggctccg aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc
    11101 ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat
    11161 ttgcctctac ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg
    11221 tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat
    11281 gcactccgtt attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca
    11341 gaagattttg tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat
    11401 cgaacgattg ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt
    11461 gtcactccat ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat
    11521 gatgcaatgg aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct
    11581 cgtgcaaaca aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag
    11641 aaagctgaat tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata
    11701 ttacttcagc caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac
    11761 tctgaaacaa tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta
    11821 gttgcaaaag gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt
    11881 caagaaaaaa tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa
    11941 gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta
    12001 gaactaaaag tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg
    12061 caattccgac tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa
    12121 ttttatcctg atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa
    12181 ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat
    12241 ggctctttac aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag
    12301 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
    12361 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
    12421 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
    12481 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
    12541 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
    12601 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
    12661 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
    12721 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
    12781 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
    12841 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
    12901 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
    12961 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
    13021 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
    13081 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
    13141 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
    13201 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
    13261 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
    13321 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
    13381 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
    13441 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
    13501 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
    13561 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
    13621 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
    13681 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
    13741 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
    13801 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
    13861 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
    13921 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
    13981 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
    14041 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
    14101 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
    14161 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
    14221 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
    14281 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
    14341 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
    14401 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
    14461 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
    14521 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
    14581 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
    14641 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
    14701 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
    14761 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
    14821 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
    14881 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
    14941 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
    15001 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
    15061 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
    15121 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
    15181 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
    15241 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
    15301 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
    15361 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
    15421 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
    15481 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
    15541 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
    15601 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
    15661 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
    15721 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
    15781 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
    15841 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
    15901 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
    15961 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
    16021 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
    16081 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
    16141 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
    16201 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
    16261 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
    16321 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
    16381 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
    16441 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
    16501 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
    16561 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
    16621 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
    16681 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
    16741 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
    16801 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
    16861 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
    16921 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
    16981 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
    17041 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
    17101 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
    17161 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
    17221 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
    17281 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac
    17341 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
    17401 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
    17461 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
    17521 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
    17581 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
    17641 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
    17701 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
    17761 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
    17821 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
    17881 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
    17941 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
    18001 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
    18061 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
    18121 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
    18181 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
    18241 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
    18301 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
    18361 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
    18421 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
    18481 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
    18541 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
    18601 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
    18661 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
    18721 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
    18781 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
    18841 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
    18901 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
    18961 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
    19021 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
    19081 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
    19141 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
    19201 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
    19261 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
    19321 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
    19381 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
    19441 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
    19501 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
    19561 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
    19621 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
    19681 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
    19741 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
    19801 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
    19861 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
    19921 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
    19981 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
    20041 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
    20101 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
    20161 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
    20221 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
    20281 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
    20341 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
    20401 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
    20461 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
    20521 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
    20581 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
    20641 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
    20701 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
    20761 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
    20821 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
    20881 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
    20941 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
    21001 aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca
    21061 gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc
    21121 atctttttga agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac
    21181 taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt
    21241 tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga
    21301 gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt
    21361 caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa
    21421 tttttccggc aaacgttcta caaattcata catataagag gcttttaggg catcattgat
    21481 ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa
    21541 tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga
    21601 gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag
    21661 caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc
    21721 tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga
    21781 aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg
    21841 caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa
    21901 agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact
    21961 tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc
    22021 caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga
    22081 cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt
    22141 tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg
    22201 gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc
    22261 gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga
    22321 acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat
    22381 gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt
    22441 gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg
    22501 aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta
    22561 agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat
    22621 accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat
    22681 catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat
    22741 gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa
    22801 tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat
    22861 gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca
    22921 gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt
    22981 tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag
    23041 tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca
    23101 atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt
    23161 tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg
    23221 attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt
    23281 tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg
    23341 agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg
    23401 acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca
    23461 atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca
    23521 gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa
    23581 tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc
    23641 acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc
    23701 agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag
    23761 ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg
    23821 atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc
    23881 gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg
    23941 cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt
    24001 tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag
    24061 aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg
    24121 accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa
    24181 gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa
    24241 tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct
    24301 gtaccaaatc cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga
    24361 ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc
    24421 atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca
    24481 gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa
    24541 cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa
    24601 gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa
    24661 tgaaagtttt attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag
    24721 aaaatgagaa aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc
    24781 tataaatgaa ttctgcataa aaaatatatc aagtaaa
//
LOCUS       DAJSVD010000033        23617 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSVD010000033 DAJSVD010000000
VERSION     DAJSVD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.033"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ON498_001194"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ON498_001194"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3939458.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ON498_001195"
     CDS             1011..2258
                     /locus_tag="ON498_001195"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3939459.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ON498_001196"
     CDS             2334..2678
                     /locus_tag="ON498_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939460.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ON498_001197"
     CDS             complement(2795..3121)
                     /locus_tag="ON498_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939461.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="ON498_001198"
     CDS             complement(3736..4938)
                     /locus_tag="ON498_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939462.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="ON498_001199"
     CDS             5055..5570
                     /locus_tag="ON498_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3939463.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="ON498_001200"
     CDS             complement(5769..6215)
                     /locus_tag="ON498_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3939464.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ON498_001201"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="ON498_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3939465.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="ON498_001202"
     CDS             complement(8094..8723)
                     /locus_tag="ON498_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3939466.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="ON498_001203"
     CDS             complement(8707..9171)
                     /locus_tag="ON498_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3939467.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="ON498_001204"
     CDS             complement(9658..10926)
                     /locus_tag="ON498_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3939468.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="ON498_001205"
     CDS             complement(11157..11894)
                     /locus_tag="ON498_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3939469.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="ON498_001206"
     CDS             complement(11961..12530)
                     /locus_tag="ON498_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3939470.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="ON498_001207"
     CDS             12658..13530
                     /locus_tag="ON498_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3939471.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="ON498_001208"
     CDS             13872..14744
                     /locus_tag="ON498_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3939472.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /locus_tag="ON498_001209"
     CDS             14741..16699
                     /locus_tag="ON498_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3939473.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="ON498_001210"
     CDS             complement(17031..17882)
                     /locus_tag="ON498_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3939474.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="ON498_001211"
     CDS             18005..18472
                     /locus_tag="ON498_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3939475.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="ON498_001212"
     CDS             complement(18735..19217)
                     /locus_tag="ON498_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3939476.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="ON498_001213"
     CDS             complement(19317..20180)
                     /locus_tag="ON498_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3939477.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20778..21653
                     /locus_tag="ON498_001214"
     CDS             20778..21653
                     /locus_tag="ON498_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939478.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21798..21950)
                     /locus_tag="ON498_001215"
                     /pseudo
     CDS             complement(21798..21950)
                     /locus_tag="ON498_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22218..23549)
                     /locus_tag="ON498_001216"
     CDS             complement(22218..23549)
                     /locus_tag="ON498_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3939479.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         7262 a         4780 c         4190 g         7385 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt ttctcacatt
    20641 ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg attatcaaat
    20701 atgtaaaatc agctactttt ttcttaaacg tttctttata ctaaatataa ataagtgttt
    20761 catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac gttttggaac
    20821 gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca agcaagtact
    20881 tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg gatgaaaaga
    20941 ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg
    21001 ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca ctttccgaac
    21061 tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta acagagattc
    21121 ttatagaaca tccgcaattt atcgaacgct tcaaagttaa gatgccattt catgcaataa
    21181 attattcaga taaattaaca actatcatcc ataaatacga tcatattatc aagaaaaaca
    21241 aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg
    21301 aatcgctcat tcagcaaaat gcctatcttc ccgcattcga attgatcaat gaagtgattg
    21361 ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt ctgatagagg
    21421 actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa aaaaggcaaa
    21481 tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca aaacgctact
    21541 ccataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa
    21601 tagataaaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    21661 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    21721 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    21781 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg gtagattttc
    21841 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccactgca
    21901 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ctctaatctt
    21961 taaagataaa tattaaaata acgattaata atattaacag tctttatttt tcaccaccaa
    22021 aaacctatct ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa
    22081 aataatgatt atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa
    22141 atatgatcct tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt
    22201 tgttatctca tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca
    22261 attcgtaaaa acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca
    22321 gctgctcgcc agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa
    22381 ggaatttgca ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc
    22441 aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc
    22501 aatccaaaaa taataggttc gcttatattg aatatagcgg gagttaccga taatttagca
    22561 aagtccttat aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta
    22621 tttcccgatc cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga
    22681 atttgctctt tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga
    22741 tctaacaatg aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca
    22801 atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta
    22861 actatcgtgg taattagctt atttatatcc atattaaaca attgaatgat tataaatgac
    22921 aataaagcaa acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa
    22981 acagcgggag gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta
    23041 ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct
    23101 gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta
    23161 aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca
    23221 atatgatttg tataatttct gcttgcacac atatgataag atactgctgc tacaactaat
    23281 aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc
    23341 aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta
    23401 ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc
    23461 aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat
    23521 acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga
    23581 ttactttttc cacccaaata atacatcact tatcaaa
//
LOCUS       DAJSVD010000034        23530 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSVD010000034 DAJSVD010000000
VERSION     DAJSVD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.034"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..305
                     /locus_tag="ON498_001217"
     CDS             6..305
                     /locus_tag="ON498_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3939480.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ON498_001218"
     CDS             378..1739
                     /locus_tag="ON498_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3939481.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ON498_001219"
     CDS             1761..2021
                     /locus_tag="ON498_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939482.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ON498_001220"
     CDS             2035..2787
                     /locus_tag="ON498_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR3939483.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ON498_001221"
     CDS             complement(2881..3486)
                     /locus_tag="ON498_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3939484.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ON498_001222"
     CDS             complement(3539..4513)
                     /locus_tag="ON498_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR3939485.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ON498_001223"
     CDS             complement(4891..5610)
                     /locus_tag="ON498_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3939486.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ON498_001224"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ON498_001224"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3939487.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ON498_001225"
     CDS             7161..8558
                     /locus_tag="ON498_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3939488.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ON498_001226"
     CDS             8639..9079
                     /locus_tag="ON498_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3939489.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ON498_001227"
     CDS             9227..9553
                     /locus_tag="ON498_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3939490.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ON498_001228"
     CDS             9554..10303
                     /locus_tag="ON498_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939491.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ON498_001229"
     CDS             complement(10383..10838)
                     /locus_tag="ON498_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3939492.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ON498_001230"
     CDS             complement(11265..11540)
                     /locus_tag="ON498_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939493.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ON498_001231"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ON498_001231"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3939494.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ON498_001232"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ON498_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3939495.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ON498_001233"
     CDS             14770..17436
                     /locus_tag="ON498_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3939496.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ON498_001234"
     CDS             17800..18552
                     /locus_tag="ON498_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3939497.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ON498_001235"
     CDS             complement(18574..19728)
                     /locus_tag="ON498_001235"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR3939498.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSDFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ON498_001236"
     CDS             19913..22354
                     /locus_tag="ON498_001236"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR3939499.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="ON498_001237"
     CDS             complement(22684..23388)
                     /locus_tag="ON498_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939500.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7521 a         4127 c         4872 g         7010 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagagga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaaa tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa
//
LOCUS       DAJSVD010000035        23238 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSVD010000035 DAJSVD010000000
VERSION     DAJSVD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.035"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..675
                     /locus_tag="ON498_001238"
     CDS             <1..675
                     /locus_tag="ON498_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3939501.1"
                     /translation="VAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVI
                     ISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYF
                     ASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYN
                     IPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFD
                     SDSALF"
     gene            687..1607
                     /locus_tag="ON498_001239"
     CDS             687..1607
                     /locus_tag="ON498_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939502.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1648..3105
                     /locus_tag="ON498_001240"
     CDS             1648..3105
                     /locus_tag="ON498_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3939503.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3450..4064
                     /locus_tag="ON498_001241"
     CDS             3450..4064
                     /locus_tag="ON498_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3939504.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4057..5781
                     /locus_tag="ON498_001242"
     CDS             4057..5781
                     /locus_tag="ON498_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3939505.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5778..7229
                     /locus_tag="ON498_001243"
     CDS             5778..7229
                     /locus_tag="ON498_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939506.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7702..9855)
                     /locus_tag="ON498_001244"
     CDS             complement(7702..9855)
                     /locus_tag="ON498_001244"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR3939507.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9857..10903)
                     /locus_tag="ON498_001245"
     CDS             complement(9857..10903)
                     /locus_tag="ON498_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3939508.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11053..12342
                     /locus_tag="ON498_001246"
     CDS             11053..12342
                     /locus_tag="ON498_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR3939509.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12355..15057
                     /locus_tag="ON498_001247"
     CDS             12355..15057
                     /locus_tag="ON498_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3939510.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15042..15899
                     /locus_tag="ON498_001248"
     CDS             15042..15899
                     /locus_tag="ON498_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3939511.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15970..16869)
                     /locus_tag="ON498_001249"
     CDS             complement(15970..16869)
                     /locus_tag="ON498_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3939512.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            17038..19209
                     /locus_tag="ON498_001250"
     CDS             17038..19209
                     /locus_tag="ON498_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3939513.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19277..19618
                     /locus_tag="ON498_001251"
     CDS             19277..19618
                     /locus_tag="ON498_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3939514.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19894..21639)
                     /locus_tag="ON498_001252"
     CDS             complement(19894..21639)
                     /locus_tag="ON498_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939515.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIELAP
                     IARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFTGIL
                     ALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVETYH
                     GKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTLLNL
                     LIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKNVSK
                     ERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKIILLD
                     EATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIGTYE
                     KLLAQKGYFYRLKNE"
     gene            complement(21650..22486)
                     /locus_tag="ON498_001253"
     CDS             complement(21650..22486)
                     /locus_tag="ON498_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3939516.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22488..23171)
                     /locus_tag="ON498_001254"
     CDS             complement(22488..23171)
                     /locus_tag="ON498_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR3939517.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7637 a         4155 c         4782 g         6664 t
ORIGIN      
        1 gtagcattta tccttttgaa tctgctgatc tcagtcgtat ttgcaatcat tatgagcgag
       61 ttgagaaata aacgactcgt taaagtttat caaacgatgt ccttactacc ttattttcta
      121 tcttgggtca ttatcagtta ctttgtttac gcttttttaa gtcctgacaa aggaatattc
      181 aatcagtgga tcactggaca tggaggagaa gctgtcaatt ggtataatga accgaaatat
      241 tggccgttta tcttggtgtt cattggtaca tggaaaggga ttggttataa cagcattatt
      301 tactttgcat ctgttatggg aatcgatccg acttattatg aagctgctat ggttgatgga
      361 gctagcaaat ggcagcagat caaacatgtg acgataccac aacttattcc actaatgacg
      421 attttaacga ttttagccgt aggaaacatt ttccgagctg attttggcct gttttataac
      481 attcctagga attccggtgc attatatgat gtgactcaag ttttggatac gtatatctac
      541 aatggcctga catccacagg agattttggt atgacggctg ctgcaggact ttaccaatca
      601 gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg cccgacgttt tgatagcgat
      661 tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa agtggagaga gtccaagtcc
      721 gctctttcaa taagacggct aattttttct ctaatataat gattgcactc tttgctattt
      781 cctgtatttt gccatttata tttgttatcg ccatctcttt taccgatgag tccatattgt
      841 cgactcacgg gtacagtttt tggccagaaa agttcagtat atttggttat acgtacttgt
      901 tcgaccagat gcaggataaa attttccaag cgcttttcgt cactgtactt gttactgttt
      961 taggtacact gatcaatagc acggctactt ctttatatgc ttatgcgatt tcacggtcaa
     1021 actttccatt ccgtcgtttt tttaccgttt tttgtttgat tacgatgttg ttctcaccag
     1081 ggatggtggc caattatctt gttatgacaa atcttcttca gttaaaagat acgatttggg
     1141 cattgatctt gccaatggct gtcagtccct tcaatattat tgtgatgcgc acattcttta
     1201 aacgttcggt ctctgattcg attattgaat cggcacggat cgatggagct agcgaactgc
     1261 gaatatttgt tcagatcgtt ttaccgttag ctgttccagg tattgcgaca atcagtttat
     1321 ttgccgcatt gggttactgg aatgactggt tcaatgcttt actctacatc caagatgata
     1381 agctagttcc actacagtat cttttgatga aaatccaaag taatattcaa tatctgacac
     1441 aaaattcagg agcaggaagc caattgacag gcggattagc ctcagttcca ggagaatctg
     1501 ccagaatggc gatcgtagtc atctcgacgt tgcctattgc gatcagctat ccatttttcc
     1561 agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa agaataaaga aacaaaaata
     1621 taaatcataa aattaggagg aagaaaaatg aagttatgga agaaactagc ctttacaggt
     1681 gtttcagcgt tgttgttggg aacattagca gcatgcggag gttctggttc taaagaccag
     1741 gcagaagcta gcaattcgga cactttgcag atgtatcaaa tcggagataa accagataat
     1801 ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa aagagatcgg tgtaaaagtc
     1861 aatatcaatt acattggctg gggcgattac gaaaagaaaa tgaacgtcat catttcttcc
     1921 ggggaaaatt atgatatcgc ctttgcgaac aactatgttt ccaatgccca aaaaggagcc
     1981 tttgctgatt tgactgaatt agcgcctaag tacgctgaaa aagcttataa tgatttagat
     2041 gaagcatata ttaaaggaaa tctggtcaat ggaaaattat atgctttccc agtcaatgga
     2101 aatgtctttg ctcaacaagt acttactttt aacaaagctt tactagataa atacgatctt
     2161 tcaattgatg gaatccaatc ttacgctgat gcagaaaaag tcttgaagga attccatgaa
     2221 aaagaaccaa acacggcagc ttttgccatt ggacaaggct tcaaagtcca agggaacttt
     2281 gattatcctt tagggaatac gttaccattt gctattgatc tggatggaga tgattcgaaa
     2341 atcatcaacc aatacgataa tgaaacgtat atcggactgt tacgtacaat gcatagctgg
     2401 tacaaacaag gactcattcc aagtgacgct gcaacgagca ataaggatta tccactagaa
     2461 ggaaatactt ggctaatgcg tggagaaaca caaggaccgt atgattatgg cgatacaatt
     2521 ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa ttactgttcc actaaaatct
     2581 acagctcaag cacaaatggc aaattttgtc gtatcaaata cttcaaaaaa taaagaaaaa
     2641 tcagtagaac ttctagggct gcttaacagt gatccagaat tattgaatgg gcttgtttgg
     2701 gggatcgagg gagaagcatg ggaaaaagtc gatggttcag atgataaaat caaattgcta
     2761 gatggttatc agccaaatac acatatgtct gcttggaata caggaaacaa caagattcta
     2821 tatacacaag agtctattac ggatgacatg attgccaaac gtgatcaatc gattgctgat
     2881 gcagaaactt caccgatttt aggattcagc ttcaacactg acagtgtcaa aacagaatta
     2941 agtaatattt ctaatgtcat gaatcaatat ttagatggat tgaatacagg gactgttgat
     3001 ccagatgaaa cattaccgaa attgaaagac gctttggaca aggctggcta tgataaggta
     3061 ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag aataataatg aataaagaca
     3121 aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc gaataaacgg tgttcaaaag
     3181 ccagtttctc ggtattaagc taaatatatg taaaaatgtg gaaagccata ttcacaaaat
     3241 ttttcttact actcgaaact aagcggctat ttcacaaact cttggtaaac ggtgttcaat
     3301 cagctgaccc tcggtattcg ctcacaaaac gacaaaatat gaggaactat ttttccgttt
     3361 gtttctctaa taatcaggtc aaggtgctga ttgcccaacc tttttttgta taatgagaaa
     3421 gtataaactt aatgtttgga ggagaagata tgttagggaa ggttttggaa gagttattta
     3481 tccggatctg ggtcattata aaactaacct tgtatttctg gatatataca tttgccggcg
     3541 ggatcatctt tggattaggt gcggcatgga aaacagtcaa tgaactattt tatttgtacg
     3601 gattcgagta taaagaaatc acaataaaaa gaggctggaa tatatataaa agaaattttt
     3661 taagaggcaa tctgctattt agtttatttt tatctggtac tgctttattg agttacaatt
     3721 tatatttatc tgttcaaatc aaagggctca tttttttagc tattgatttt attttacttt
     3781 ttgccttgct ctgcctattt gctacttatc aatacagtat gattttagat agtgaatata
     3841 cgatctctgt tcctaatctg ctaaagctaa gttttatttc ggttttttcc agcttttcgt
     3901 cttttctaaa aataattatt ggtagcggta taattttagg cgtgacttgg caattcaaag
     3961 gattgatcct gtttggtgtc attggtcttt tgacggtttg gaatggtacg atgacaacac
     4021 attggcgtga agaattagat aaacagttgg aaagttatga ataagtttcg gattctattt
     4081 tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg cggttatcat gatcggtgta
     4141 acgatgactg ctaccgttat ttttagtatc catacttggg aacaaaatca aaaacaggca
     4201 gaaaacatga caagtgatgc cgttcaaagc acaagcagaa tgctgaatga taaaacaacc
     4261 ttgagtagga tcatcaaaaa ccagcttgta gggaattccg aaaagatcga aaatgtgacg
     4321 acctacttaa caaaacccat agatcagtat ctgatgtatg tatacgaaca gcaaaacagt
     4381 accgatgaat tagtttcttt cccaaatcaa atcaaagatc tctacgcaaa ttatgaagaa
     4441 ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt attatgaatc ttctagagaa
     4501 aacaaaggtg gggaaataaa aaaggggaca ccaaaattac tagacgcctt ttatatcaag
     4561 ttgcctatca cacaaggagg agctgagtca ggaagtattt ttattggttt tcaaaagaaa
     4621 gaactagata tgattttaga aaatctaacg tcattcaatg gactctcttt atacatgatt
     4681 tctggaacga caaatcgact atacacattc catgataata aaatagctaa agaaacgttt
     4741 gaaagacaag aaatgatcat cacagagagt ctgaagaaaa attcacaatt gccgattgac
     4801 gatctggaaa aaaacaattt tgttcaacac caagaactgt ctgatgatta tcgtatatta
     4861 gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa aaaatcttgc cccgctgatc
     4921 attggtatca ttattttaga caatatactg cttatctttc tttataaaac attcaaacga
     4981 tattcacagc aagtatctat cgtcatgcag gccatggatc aaacagcagc agggaattta
     5041 gagacgcgga tcgacataac aaacacggaa tatgaactga aggaactttc aatcggtatc
     5101 aacgaaatgt tggatagcat caatcagttt gtagaagaca tctacaaatt agaaatcaaa
     5161 cagcaagacg cacatatgcg ggcgcttcaa gcacagatca atccacactt tttatataat
     5221 acattagaat atatccgaat gtatgcaatc agcgaaggaa gtgaagaatt agcagatgtt
     5281 gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc aagagaaaac gatcacttta
     5341 aaagaagaac tggacttttg cgaaaaatat gtctatttgt atcaaatgcg ttatcctaat
     5401 cgagtagcct atcattttat gatcgatcct gatttggaaa agatcgaagt acctaaattt
     5461 gtgatccagc cattagttga aaattatttt aaacacggta ttgattttac tcgattcgat
     5521 aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg taaggatcat catcaaagat
     5581 aatggaaaag ggatgactga aaaacggcta aaacagattg aagaaaaact gtctcatcct
     5641 aaagtggaac ttcacggttc tatcggtctg caaaacgtga atgaacgttt acgtgccagc
     5701 ttcggcagca gttattatat gtcgcttgag aataatgaga caggtggact gaccgtttct
     5761 attacattca aggagggatg agcgtgtatc aagtgttatt ggtagatgat gaatacatga
     5821 ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt aggctttcaa atagtagcaa
     5881 cagcagagaa tgccaaagaa ggactagctg tacttgaaca gcgacagatt gatttagtag
     5941 taacggatgt gacaatgcca gaaataaatg gactggaatt tattgaagcg gctcaaaaag
     6001 aacgtcataa ttttgaattt atgattttat caggatacca agaattcgat tatttgaaag
     6061 gaggtatgca attaggcgcc gtgaattatt tgatgaaacc cgtgaataag ttcgaattgg
     6121 ttgaaagcct caagaaaatc aaaacacggt tagatcagca aaatgaacaa aaaaatcaac
     6181 aagaaatcta tcaagaactg ttattcagtc aatggttgaa tgaagaattg gatgaaataa
     6241 gtgaagaaga aataatagga caaataagtg agaagcagca taggatagta ttgattcaac
     6301 tggcaagaac tcatgatact cttatcaata gatggttgca agaacggcaa caaatctttt
     6361 actatcaacg aaattacgga gaccgtgtat tgatcacgtt gttgttggag gcagatgaag
     6421 ttgatggctt ctgttgtttt gttcaagaaa actttcctga tcaggaatgg ctgatcagta
     6481 tcggggaaga gacagattct ttggacaaaa tccctgaaac attccaacaa gcgaaagaca
     6541 atctacagtt gcatcaattt tatggagata aagacgagca tgttttttac gcagtacaag
     6601 cagaaacaaa agatcaaacc atcgattttt caagtttcag acgcgtgctg caaaataaac
     6661 aattggaagt cgctcaaaaa atgatcactg atttttttga acagtttcag ttagcagcca
     6721 tgatgccgga agatatacga tattccagtt ttttactgtt tatggagatt caacgggaat
     6781 tgattgatct ggaagatgag gaatatttac aaggaatcga aaaaatccat caggcaaaaa
     6841 cggtgcaaga acttcagcag ctacttctgt cattcatcaa aaagcatcag agacagaaaa
     6901 agtacagtga caatgtggaa aaagtaatcg taatcctgca ccagcattat caagaaccac
     6961 ttacactcaa agaagtgtca gaaaacctgc atttaaatgt catgtatcta ggacagctgt
     7021 tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa ccatttacgt atggaaaaag
     7081 cgaaacaact gcttttgcac agtaatcaaa acatcaatga gatagcaagt gagattggct
     7141 acaacaacac cacgtatttc tctaaattat tcaagaaaat cgtcggcaga tcccctaaag
     7201 agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc aatgtaatga aataacttat
     7261 aaaaaattgt ctagaaatct aattttgggc tgttcttatt acttgaggca aggcagcttt
     7321 ttcatgacct ctcgataaac ggagttcaaa agccagtttc tcggtattaa gccaaattta
     7381 tgcaaaaatg tggaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct
     7441 ttttcacgac ctctccacta aatggtgttc aatcagctga ccctcggtat tagctcaaaa
     7501 aatggcaaaa tatgaggaac tattttgcca tttgtctctc taatactcag gtcagaacgc
     7561 tgatttcaca acctctcaat aaacggtgtt caaaagcttg catctgcggt aataagacca
     7621 accaaacgaa aaatatgagg atctattttt gcttggctgt tcttattact tgatgcagga
     7681 cagctttttc acaacctctt gttaattata gtttgataag gaaaaaggca aatcttttgc
     7741 ttgatatttc ttagggtgag ggatgatccc taattggtaa gagatcgtgc cgcctttcag
     7801 caaatcttga tgggtaaaat ataatttcgt atattctctg tccatccgtt tgattttatg
     7861 gataaattgt tgctgcggcc gattaggcgt tgcttcaatc gtcaattcct ttccatttga
     7921 caaaagcaaa acagccttgt caattaatgg cataccgata acatattcac cggttccagg
     7981 cgtgacagga taaaatccca gactattgaa gatataccaa gcggccatac ttccattgtc
     8041 ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc tgagtcatca actgtttaag
     8101 cagtggctgt gccatttcag gttgtcctat ataactgaac aagtaaggat agtgaaaact
     8161 tggctggttt gaaatagcta attgtccaaa ttctatcgcg gccatctcac tcatttcgtg
     8221 gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc tgattgcaca gctcaatgag
     8281 tttttcttta tatctctcac tgccaccata ttttccgatc agtcctgaaa aatcgtgata
     8341 aacagcaaag ctgctttgcc aagcacttcc ttctgcataa tcttgtcccc aacgattagc
     8401 tgaaaatggt tctctgaact tgccatcttt atcttttgaa cgcataaatc ctgactcata
     8461 gtcaaagata ttttgatagt tcaatgattg ctgctgatat ttcttcgtca tttcctcttc
     8521 ttctagtacc ttggcaactt ggctaataca aaaatcgcta taagcatagt ctaatgtgtg
     8581 attgactgat tcgtggtagt gattaggaac atagccatat gtcaagtaat cttctgttcc
     8641 ttggcgtcca taatttggat gtttgctttg aaccgtagca cctttttcca ttgccgttag
     8701 aaattcaggc atcaagtctc gtcggatatc ttttacagca gcatctgcaa tgaccgcatc
     8761 gatcaatgtc ccaggcataa gtccacgttc atcaggagac agccattttg gcaaatagcc
     8821 actttctcta tagctgttca aaaaaccttc cagcatttct tcgtattttt ctacagcaat
     8881 caaagaataa agaggataaa ccgttttata cgtatcccaa aacccattat ttgtatagag
     8941 aacaccaggt tttatagatc gactagtcgt atcatagtgg attttttgcc cactaggatc
     9001 aatttcataa aatgtttgtg gaaataaaaa cacccgatat aaattatgat aaaacgtccg
     9061 caattgttgt tgatcatggt gtgtgagctg tatacgatca aaataggact gccattgttc
     9121 ttgactgtga gcaaggtaag tctctttgct ttggttttgt tctagttcca ggttataagc
     9181 tgcttgttcc gagctgataa aagatgtccc aaatcggata gtttgttttt ttgtctgttc
     9241 aaaatacaac gggatcgagt cgctattttt gttggaaaca gctaactcat caattgttaa
     9301 aggatattca aatctcaata taaaataaaa agaaaaatca ggatcttctg aaccagcaaa
     9361 atgtgtgatt tttccttcca ctgtgtaagg atctataaca aagagctgat ggcgtcccgg
     9421 aaaggataaa agtaaaccgc tgtcattttt gctgtaatca atagataaga ttccgccata
     9481 catgctaggt atcaatgtag aacagatgcc ataacgtaat tgacagatcg acagatgagt
     9541 cgggcaaaat atactttctt ctgggcggta agaactttga gcatggaaaa gggtgttctc
     9601 ctgtagtttt ccattgatcg gcgtaaacag catatgactg aaatcaccca tccaaggact
     9661 tggttgatgg gtaagacggt agccttgaaa aacgcggtca tcaggatgaa accaccagct
     9721 gccgttttga tccgtagttt gcggtgcaaa ataattcatc ccaaaaggaa cgccagtata
     9781 aggctggcag ttcccattag agaaatttgg ctgattcgct gttgcatgtc tagtatctat
     9841 ttgaccgata ttcattttat ttctcctttg ttgattgtct tttgatgaat gaaactggaa
     9901 atttactatc ggatggtcga tcttttgtct ctaaccaagt gaccaaagca tctcccgcaa
     9961 gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag tggcgggtca acaagagctg
    10021 ctgcttggat atcatcaaat ccgataattg aaaaatcttc agggatatga taaccatgct
    10081 gtctcaagag attcatggtc tggatagcga caacatcgtt ttcacaaaca aaagcagtta
    10141 cttcatactt agtgatataa gaaagcaatt catctaatgt cgcgcttttg tcatccagtg
    10201 aagtgtggaa agtcaaacca gctttgtcca atgcttcaag gtaaccaagg taacgctgtc
    10261 gaacggattg tggatgagtc tgcgtaccaa acaaataagc gatttttgaa tgtccctgtt
    10321 cgatcaaata agaggcagca agcgcaccgc cttggaaatt atcggaaaga acggtaggaa
    10381 aaggtaactc atagattttt ttatctaata tgacgacagg gaattctttc aatgaaagct
    10441 caaaaagtaa gtcaagatgt tgttctgtat caagagcata ataaatcaat ccgtcaaatt
    10501 cattgattag ctcttttgct gttttatttt gtagaaagtc aagcgtcgtc atcatgacat
    10561 caatctgttt atcttgcatc acagggttca atcccttgct aaaatcacca acagacaaat
    10621 cagcgacata tggaagaaga aacagaatcc gtcgtgtttt tgtaggagaa gcaagaatct
    10681 gttccggaga agcggattta acgaaactgc ctttcccgcg aacgcgataa ataagtccta
    10741 attgctctaa ttcagtcaat gcacgtttgg aagtgattct gcttacttta taggtacgtg
    10801 aaagttcttt ttcagttggt agttggctgt ctacaggtaa ttcaccagtt aaaatagccg
    10861 tttgcaaatc attaaatatt gtctggtaca atggttgagt catataaaca cctcttaatg
    10921 atatatcata ttcaaataat accataatgg atgaaaaagt aaataaaaag atattttttg
    10981 aaagcgtaga cataattggc tgtatgaagt ataatcttta aagatatatc aaatagaaag
    11041 aaggttttat tagtggttta taaagatata ccaaaatcgg tgcagaaatt tatggaagat
    11101 atcacacaaa aatgtgggga tatccatgcg gactgggcaa agaattttac agcagcattt
    11161 gccaatactc tgttgacaac agtcaaaaag caaaatgacg gaaccacctt tttattgacg
    11221 ggagacattc cagcgatgtg gttaagagac tctactgcac aagttcgtcc ttatttagtg
    11281 attgcaaaag aagatgagga attagctgac atgatcagcg ggttagtcaa aaagcaattt
    11341 ttttatatca atatcgatcc atatgcgaat gccttcaacg aagaagctaa cggtgctggc
    11401 caccaaacag atcatacaaa aatgaacgat tggatctggg aaagaaaata tgaaattgat
    11461 tctttatgct acccaataca gttggcctat cttctttaca aaaatacagg aaaaactgat
    11521 caattcaatc aatcttttat tgaaggtatc aaaaaaatct tggacgtgtt caaaacagag
    11581 caagatcatg aacactctcc ttatacattt gtacgagaca cgacaagaaa agaagatacg
    11641 ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag gaatgacatg gtctggtttc
    11701 cgtcctagcg atgatgcttg ccgttatgga tatctggtac catccaatat gtttgcggtg
    11761 gtcgtgttag gttatgtgga agaaattttt caaacgatac tcgatgataa agaaattgta
    11821 cagcaaacac agaaattgaa gaaagagatc caagagggga ttcagaatca cggacgaacg
    11881 gttaatcaaa aagggcaaac gatttatgca tatgaagtag atggaatggg aaacgccaca
    11941 gtcatggatg acagtaatgt gcccaatctg atcgccgcac cttacttggg atattgttct
    12001 gaacaagagg aagattatct gcaaacaaga agaacattgt tgagtaaaga aaatccatat
    12061 ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc atacgccaga aaaatacgtt
    12121 tggccgattg cattagctat ggaaggaatg acaacgtcag acaaatctga aaaagagcgg
    12181 atattgaatt tacttgtagc aactgacgca ggaacacacc ttatgcacga aggattcgat
    12241 gtgaacaatc ctgaaaatta tacgagagaa tggttttctt gggcaaatat gatgttttgc
    12301 gaattagtca tggattactt tgatattaga gtagaaaagt aggaggtatc gtaaatgaaa
    12361 aagaaagtat atatcatttc tcatagccac tgggatcgtg aatggtacat ggcttacgaa
    12421 cagcatcaca tgcgactgat tgaattgata gacgatctac tagagctatt tgaagttgat
    12481 ccgtccttca acagttttca tttggatggg caaacgatta ttttagatga ctatcttcaa
    12541 gtaaggccag aaaaaagaca ggcaatccaa caagcaatca atgaaggaaa attgagaatt
    12601 ggaccatttt atatcttgca agatgatttt cttatcagct cggaatcgaa cgtgagaaat
    12661 atgctgattg gcatggaaga aagccgtaaa tggggaaccc ctgtcatgtt aggctatttt
    12721 cccgatacat ttggaaatat gggacagacg cctcaattaa tgaaacaagc tggtatttca
    12781 gcagcagcgt ttggccgtgg tgtaaaacca attggctttg ataatcaagt tttggaagca
    12841 gaaaactatt cttcccaata ctcagagatg tggtggaaag gccctgatca aacagcaatc
    12901 tttggtctgc tatttgccaa ttggtatagc aacgggaatg agattccagt agaaaaagaa
    12961 gcagctcttg ccttttggaa gcaaaaatta gcggatgcag aacaatatgc ctctacgaac
    13021 cacttattga tgatgaatgg tgttgatcat caaccggtac aaaaagatat atcaaaagca
    13081 atccatctcg caaatgaact gtttccagat tacgagttta ttcattctaa ttttactgac
    13141 tatctggaag cagttcaaaa agatgtgcca gaagacttag gctcagtaga aggagaattg
    13201 acaagtcaag aaacggatgg ctggtatact ttagccaata cagcatctgc tcgggtttac
    13261 ttaaaacaat ggaataccaa ggtccagcga cagctagaaa atattacaga accacttgcg
    13321 acgatggctt atgaagttag tggaaactat ccccatgatc aattagatta tgcttggaag
    13381 acattaatgc aaaatcatcc tcacgacagt atttgcggtt gttcagtcga ttctgtccat
    13441 cgggaaatga tacctcgttt tgaaaaagca gatgaagttg gaaagtatct tgcacaagat
    13501 tccttagaac agctgaccgc agcaatcgat acgaccggat tcccaaaaga cagctttcca
    13561 tttgtcattg taaatactgc aggaatggat aaaactggtg aagcagaaat aaccatcgaa
    13621 ttagaaagaa aaagattcgc tgaaggaata cctgaacaac tatatcaaga gttagaaaat
    13681 ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg ccactatacc agctatttta
    13741 tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag accgtttccg tgtgccatat
    13801 atggcaagaa tgatcaaagt gactttacca ttagaaaaca tgccagcatt ttcttgggaa
    13861 acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa aggaaacaat gattcatcag
    13921 agtggacgaa tcatcgaaaa cggccctcta catttgacga ttgaaaaaaa tggtaccatt
    13981 acaatggaag atcgcaaaaa caaacgaaag ttgaataatc tgcatatttt tgaagatatc
    14041 ggcgatatag gaaatgagta tatctttaaa caacctttct gtgatcagcc aatcctatcc
    14101 agtaacaaag aaaactctga agtaaaagta ttagtagata caccggaaat agcaaaaata
    14161 agtattctac aggaaatgga aatccctgtt tctgcagacg agcgtttaga aaaagaacag
    14221 cagatggttg tggaatttcg ttatcgaaaa gcagaacgat ccaaagaaaa acgaatcctt
    14281 caaataaaaa caatcatgac aatcagaaaa gatagtaaaa aaatcgactt tgaaacgact
    14341 ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt ttcctacaaa gttacatgtg
    14401 gaacatcacg aagcagatag tatttttgaa gtggtgaagc gcccaaacca tgtgtctaaa
    14461 agttgggaaa accccacgaa tccgcaacat cagcaagcat ttgtcaatat tcatgatgaa
    14521 gagtacggtg taacagtcgg aaactttggt ttgaatgaat atgaagtaac agaagacgga
    14581 caaatcgcag tgacgctact tcgcagtgtg ggagaactag gcgattgggg atattttcca
    14641 acaccagaag cacagtgtct aggtgagcat cggttcaatt acagcattga actacatgga
    14701 ccagaagaaa aattctctac ttatttgcat gcatacgcag cacagattcc tttttccact
    14761 caacaaatca aacatcatga aggaacattg atttcaaaac aacaatattt aacgataaaa
    14821 agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt ttagtgataa agtagtagta
    14881 agaggtttca atatgtctag tcatttagaa aaattggaaa taactaaaga caatggaaaa
    14941 actgtcatat tgaatctgct tgaagaacca acgaagcaag cggttgtccc aataatccaa
    15001 ccttatgaga ttcggacaat cggattcgag gaggaaaacg aatgtatggc ggaatagaag
    15061 caggcggaac aaaatttgtg tgtgcggttt cagatggtgg agaaatttta gaaaaattaa
    15121 gtatccctac cacaacacca gaagaaacat tggatcaggt atttgaattt tttgatcgtt
    15181 atgaattgaa gggaatcggg attggctcat tcggcccgat tggtatcgac aaaacaaaag
    15241 ataattacgg atacgttttg gcaactccca aaaaagggtg ggcacaattc gattttcttg
    15301 gcacaatcaa ggaacgctac aatattcctg ctgcttggac aacggatgtc aatgctgctg
    15361 catatgggga attgaagcaa ggagcagcac aaggaaaaaa tagttgtatt taccttaccg
    15421 tgggaacagg aatcggtgca ggtgtcgtga tcaatgaaga aattttttca gggatcgcac
    15481 atcctgaaat gggacatatt tgggtgaaac gccatccaga tgaccattat gaaggtactt
    15541 gtccttatca taaagattgt ttagaaggat tagcagcagg accgtctatt gaggcacgca
    15601 cagggatcaa agggcaagat ctgccgcaag atcatcctgt ttgggctatt caggcttatt
    15661 atattgctca agcactgatc aactatacgt tgacgttagc acctgaaaag attattttag
    15721 gcggtggggt aatgaatcag gatcatctcc ttcaaaaaat ccgtcagcaa tttgttgaat
    15781 tgatgggtgg ttatatggaa acacctcaag taagtgaata tatcgttcgt tggggactgc
    15841 cgaatgaaag tgggattatc ggtagtttgc tgttagcaga acacgaacat caaaagtaag
    15901 cagacaaatt acacaaaaag aacagagtct tatcattatc tgctgataag actctgttct
    15961 tttttatttc tattcccata atccaggtaa agctttaccg actatttttt cttgagtaaa
    16021 tggatggatc atgtgcagtt ctaatgcatg gagcatcagt cgttcagctg ttctattttg
    16081 gtagagaggg tctccaacga tcggatgtcc cttatgagct aaatgaactc tgatctggtg
    16141 tgtacgtccg gtttccaaaa cacaatagac ttgtgtttgt tcagaaagta ctttgttgac
    16201 agtgacatgt gtcaaggcgc tctgaccatt tttatcatct acgacccttt ttctccgatc
    16261 atggcgatct cgtccaattt tattggtgat ggtcatatct ttggcaattt ctccccatac
    16321 gatggcttga tatctccgat agatcatttt ttgttccaac attcttccaa gtatcggcaa
    16381 gacaaatgga ttttttgcaa ataaaacggc accgcttgtt tctttgtcca agcggtggac
    16441 aacgtaaggt cgttgctgtt tagtgtcgag ataagcagct aaatgattga gtaaggtact
    16501 gttttctgtc ggttcatttg gatgagtctt cattcctgca ggcttatcca ctataatcaa
    16561 atgctcatct tcatataaaa cacggatgtg cttcgcatct cctaattgaa cttcttgata
    16621 attataatct gtttcttcga ataacaatgt gatctggtca ttgcccttta cttcatgatg
    16681 gaacaaagca ggttgttgat tgatccaaac gtttttacgt atacgcaaaa agtgtcgaac
    16741 ctttctagga actaaccatt ccttttcaag caagtcgcga attgttgttt caggatggtt
    16801 cataggtaaa gtgatcgtga ttttcacaag tgtactcctt tcagtgcaat tgcagactaa
    16861 ttgtaccatt tttaaaacat atttaagaaa aaacttacat ttttctgttc attcttatgt
    16921 tactctactt gcactaacgt aaaaaaacat attgaactac aacttaagca ggaagaaaac
    16981 actctttcac tatggtaaaa taagacgatt gaatcaaata gacaggagaa gaagcaaatg
    17041 gattttcaaa gcattttaga aaagataaaa gctgctttga tcagtttttg gcgttggatc
    17101 agaccatatc tcggacaatt ccatcgatgg cgaaagcgca tctggaaaaa atatcatgtg
    17161 aacaaattga ttttgctgat tggtttgatc tgtgtcttag tgacaagtat ctacctcttt
    17221 attttagcaa aacaagcaaa tgtcgaaacg ttaaaatcag gattaagcca atcgacagtc
    17281 atttatgata agaaaaacga agaagctggc acactgtacg cccaaaaagg aacatatgta
    17341 gaattaaacg cgatttctcc ctatatccaa gatgcagtaa tctcgacaga agataggaat
    17401 ttttacgagc atcacgggtt tgatatcaaa gggattgccc gtgcagcagt ccgaatggtc
    17461 ttgtctggcg gaacatcagg gggcggagga agtacgatca ctcagcagct tgccaaaaat
    17521 gcgtatctga cattagacca aacgtttgat cgaaaagcga aggaattatt tttggcaatc
    17581 gagatcgaaa agaagtacag caaagaagaa atcttgacca tgtatcttaa caatgcctat
    17641 tttggcaacg gggtatgggg tgtccaagac gcttccagaa agtattttgg agtagatgca
    17701 acgaatgtta cgctgtctga ggctgctact ttggcaggaa tgctgaaagg tcctggtatt
    17761 tataatccga tcgatcaccc aaaaaatgcg aatgatcgga aaaacacagt actaagtgta
    17821 atggaagaaa acggaaaaat cacaaaagaa caagaacaag cagaaagcca gacagatatc
    17881 agcgcgtatc ttaatgacac atacgataat tcagattctg gttatcgtta tccttattat
    17941 tttgatgcag tgatcgatga agcagttaac gagtatggtc ttgatgaaga agatgtcatg
    18001 aataaaggct ataaaatcta tacagcttta gatcaggatt atcaaaagca attagaagct
    18061 acttatcaaa acagtgcatt attcccagca aatgcttcag atggtgccat ggtccaatct
    18121 gcttcagtag ctttagatcc gaatacaggt ggtgtacaag cactagtagg acgaagagga
    18181 gatcatgtct ttagaggatt cagctttgct acacagatgc gtcgttcacc aggatcaacg
    18241 atcaaaccat taagcgtata tacaccagca ctggaagctg gatacaagcc ttccagtatc
    18301 ttgaaagacg aaccgcaaag ttattacgat gcgcataatt ttgacgggac ataccaagga
    18361 gaagtaccga tgtatcaagc tgttgcacaa agtttgaatt tgcctgctgt atggcttttg
    18421 catgagatcg gtttacaaaa aggatatgat aaagcagaag aattcggcct gcctttagca
    18481 aaatctgata agtattatgg cttagccctt ggtggactgg aaaaaggagt atctccattg
    18541 atcatggcgg gtgcttacag tgcatttgcc aatgatggtc agatgtatac gccgcatctt
    18601 atcacgaaaa tcgttgattc gactggagcg gtgatcgtcg ataacacaaa caaaaaacca
    18661 aaacaggtca tatcaaagga aacagccgat gaaatgacca gcatgcttct tggtacattt
    18721 tcaaatggaa cagcggctgc tgcgaatcct gcaggttata cgatagcagg gaaaacagga
    18781 accacagaaa ctaatttcga tgccacaaaa gcaaatgacc aatggatgat cggttacacg
    18841 ccagacgtag tgatttcgac atggatgggt tacgaagaat ccagcaagct gcactatttg
    18901 gaagggacaa gtggaaatgt tgtcgggaaa gtcttcaaat cagcggcaga aggcatcctt
    18961 ccgtatactt caaaaacagg atttagtgta gcagatgcct atgccacagg aggacaagtg
    19021 gtagctgcag atcaagttgg aaacacagga aattctaatg gagagtctgg aaattggcaa
    19081 gataacttga atcaatatgg tgaacaagca aaagaagggc tgaaaaactt cggcgatatg
    19141 gtgaaagaag gcgttcaggg aattggtgaa gcggctaaag acctctggcg gaaatatcaa
    19201 ggagagtagc ccctttgaaa acgaataacc ccatgttaca atagagagta actactaaaa
    19261 gaaaagaggg cataacatgt caaacatcta cgatagcgcg aatcaaatcg aacgtgagat
    19321 ccgtgaatta ccagaattca aagcgttgaa agaagcgtac gagaaagtac aagcaaacga
    19381 agaagcacac aaactgttca aagacttcca agcgatgcaa atcgaattac aagaaaaaca
    19441 aatgagtgga caagagttca gcgacgaaga tgcaatgaaa gcacaagaaa tggctgtgaa
    19501 aatccaatca gaagaagtaa tcaatgactt gatgcaaaaa gagcaaggct tcagtacaat
    19561 catcaatgac ttgaaccgta tcatcatgac accagttcgc gacctttaca gcgaataaaa
    19621 ctattttatg aaacaacaga gggtgtgaaa gagacgttcg gccttgagcg atgacaaaat
    19681 tgcttcttat tgccgatagg ctgtctcttg aacaccattt accaagaggt cgtgagccta
    19741 ccgtttaact ctgagttaca aggaacaatt tcaaaaaaca gcttctcatg tttgatgact
    19801 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcagaa ataggagcta
    19861 cgacaaaagt cttgtcatag ctcctatttt ttgtcattca tttttcaagc ggtagaaata
    19921 gcctttttga gctaacagtt tttcgtaagt acctatttca acgatttttc cttcatcgat
    19981 cacaacgatt ttatctgcct ctttgatcgt tgaaagccga tgagccacaa taaaagtagt
    20041 gggtgtttct gtcaggtaat tcagggcttg ctggattttt ttctctgact ggttatctaa
    20101 agctgaggtt gcttcatcta gcaaaatgat ttttggctga cgtatcaacg ctcgtgcaat
    20161 ggagatacat tgcttttggc caccagacag cttgttgcca tgttcgccta ctaaggtatc
    20221 cagaccatca ggaagctggt caatcagtga agaaagttgc gcagcttcga tgacttcatc
    20281 taaacgttct tttgatacgt ttttcaagcc ataagtgatg ttctctttga tcgaagcgga
    20341 gaacaacaaa gtggtttgag gaacaacggc taaataattt cgcacacttc gcatatccag
    20401 ttcatccaaa ggctgatcat ctagaaaaat ttttcctgaa gtcggctgga taaatccaat
    20461 caataagtta aggagtgtag attttccaga gccagatggc ccaacaaaag caattgtttc
    20521 tccagctttc acctctagag aaaaatgatc gataatcggt ttgagtgagt ccggataaag
    20581 aaaagaaaca tcctcaaaac gaaaatttcc atccaaatgt tggatttttt tcttaccgtg
    20641 ataagtttct acatcgccag caatcaacag ttcgccaata gaagtgatag attccatgcc
    20701 ttttgaaatc gtaggaatca gcgtgatcat tccagaaacg gaattgacga tcgtcgtgaa
    20761 atacgtctga tacatcacga catcgccggg ttggatgcgc ccttgtaaag ctaatatccc
    20821 agtgaaactc aaacaaatca gctgaaatat ctgaaaacta gcccagctga ctgatccgaa
    20881 aacagattgc aggatatcta agtggaatcc tttttcatag atttgagtaa aatgcttatt
    20941 taagcgagtt gtttcgtgtt tttctaaccc atgagctcga gcaatcggtg ctaattcgat
    21001 cacttttgca gaagtctctt ccatttcttg cctgaagtct gagttggact gtctgatcct
    21061 tttacggaaa aagagagtaa cgaatgcggc gagaggaaca gtcaataaga aaaacatgaa
    21121 aacaacagga cttttataaa gcgtgatacc taaagctacg aataaattga cagcgatatt
    21181 taatccgctg acaaatactt gagaggacaa cgtttggata gcttctacat cacggatgat
    21241 ctttgactgg attcgtccag attgcaattc tttttgataa ggaatagata gttcttggat
    21301 tttcttgata agggtcgctc ttagtccagc ttctacttga cggatcgaag tgctatggta
    21361 ttttacatga aaataattcg tcaatatatt ttgaccaatc agacaagcca tcaaaaaagc
    21421 gttgaaataa atggcctgca actgagaaga atctttcgat gaagctgcat tgattacatt
    21481 ggcaatagcg atcggcatta accaagatgg aagatgtttg ataataaaaa agagtcctga
    21541 catggcaaaa cgccaataat ttccctgata aagatgcagc aatgtctgaa aagcatttgc
    21601 atgatatttt tgataacttt tctgtattcg ttcctccata atcttctcct taacaatctt
    21661 ggtaaataaa ctgcaagtgt tctattgctt caggaacata aatctcagga gtagcttcaa
    21721 gagtggcgta cattagagga cgctcatatt tgatataatg cagtaccggg cggaaatcta
    21781 aacagccctt gccaacaggt accatgcgga tctttccatt ttccatgata tagtctttga
    21841 gatgaagcgc tgtaatataa ccatgtaaat tgtccaaggc acctcggatc actttttcct
    21901 gatcagcatg attttcttta tgcatcaaat tcgcacaatc taaaatgatt tttaaatttg
    21961 gtgaagctac ttcatcgatt aaacgtttag ctaactgata gttatagatt ggatgattga
    22021 tccctgcttc aacagcaaca gttacgccaa aattttctgc atgagcgact aattcaatga
    22081 tagaatttct ggcgatctga taagcttctt cagtaaaatt ttcttttgtg taaccgacag
    22141 agacactacc ggtttccgta gcaatcatct tggcttgata ggcagagcac agagcaattt
    22201 gctgtttgaa agttgccaat gcttgtgcac gaacttcttg atttttgctg gataggttga
    22261 tatagcatcc taaaatggaa agttcgatat tgtttttttg gaagtattca ccaaaatagg
    22321 cagcactccc aagtgtcatg tcagttaatt taagttgttc aggaaatgct ttttgtggtg
    22381 caaattggat atgggacaag tgaagctgtg atactttttg aacgatttct tcacgatttc
    22441 gaacatctaa atcgtgggca cggatgccta aatttaatgg aaacatctca aactcctttt
    22501 tgtaaataat tagcaagagg cagacgggat tcttttatca attcaacaat tattcgggca
    22561 actttatcag cgccttctgg actaaagtgg gtattgtctt ttagtccttc tgggtaattt
    22621 ttacaagtat caggcataag atgcaggtag aatttttttg tctcttcttt tgagaaggtc
    22681 tggaacaatt cttgtgatct tgagaacaca tctaaaagag gaatatggtg tttctcagca
    22741 aatgcacgca tcgcttttgg atagtctcca agtatatctg gattcaatgt tccgttttct
    22801 aagtaatctc gtcgcgtcac agatgtcagt aagaccattt ggacgttcgc tttttttgcg
    22861 cattcaaaaa atatctgcag attttttaaa tagctaccga agggaagcgt acctctttct
    22921 tcttggattt tctggtcgtt atgtccaaat tgtacaaata agaaatcatt cggctgaaac
    22981 tgtgaagcta gacgggctaa ccttccttcc tggataaatg atttggtgct gcgtccattc
    23041 tttgcttggt tattgatctg gatctcgggt gaaaaataag cagagatttt ttctccccaa
    23101 cctgtctcgg gacgcttgtt ttcatctttt tctgccgcag tagaatcacc ggcaatatgg
    23161 atagtaggca tgttatctct tctttcataa aggtttatag gcaatgaagc agaacagcaa
    23221 gagaagaaga cggagcaa
//
LOCUS       DAJSVD010000036        23033 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSVD010000036 DAJSVD010000000
VERSION     DAJSVD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.036"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..170)
                     /locus_tag="ON498_001255"
     CDS             complement(<1..170)
                     /locus_tag="ON498_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939518.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCW"
     gene            complement(177..725)
                     /locus_tag="ON498_001256"
     CDS             complement(177..725)
                     /locus_tag="ON498_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3939519.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(770..1102)
                     /locus_tag="ON498_001257"
     CDS             complement(770..1102)
                     /locus_tag="ON498_001257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939520.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1116..1373)
                     /locus_tag="ON498_001258"
     CDS             complement(1116..1373)
                     /locus_tag="ON498_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939521.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1568..2110)
                     /locus_tag="ON498_001259"
     CDS             complement(1568..2110)
                     /locus_tag="ON498_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939522.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2131..3222)
                     /locus_tag="ON498_001260"
     CDS             complement(2131..3222)
                     /locus_tag="ON498_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3939523.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3230..5440)
                     /locus_tag="ON498_001261"
     CDS             complement(3230..5440)
                     /locus_tag="ON498_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939524.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5456..7984)
                     /locus_tag="ON498_001262"
     CDS             complement(5456..7984)
                     /locus_tag="ON498_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3939525.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8036..8446)
                     /locus_tag="ON498_001263"
     CDS             complement(8036..8446)
                     /locus_tag="ON498_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939526.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8459..8677)
                     /locus_tag="ON498_001264"
     CDS             complement(8459..8677)
                     /locus_tag="ON498_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939527.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8674..9666)
                     /locus_tag="ON498_001265"
     CDS             complement(8674..9666)
                     /locus_tag="ON498_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3939528.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9745..10656)
                     /locus_tag="ON498_001266"
     CDS             complement(9745..10656)
                     /locus_tag="ON498_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3939529.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10675..11019)
                     /locus_tag="ON498_001267"
     CDS             complement(10675..11019)
                     /locus_tag="ON498_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939530.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11039..11595)
                     /locus_tag="ON498_001268"
                     /pseudo
     CDS             complement(11039..11595)
                     /locus_tag="ON498_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11676..12176)
                     /locus_tag="ON498_001269"
     CDS             complement(11676..12176)
                     /locus_tag="ON498_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939531.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12177..12722)
                     /locus_tag="ON498_001270"
     CDS             complement(12177..12722)
                     /locus_tag="ON498_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939532.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12719..13975)
                     /locus_tag="ON498_001271"
     CDS             complement(12719..13975)
                     /locus_tag="ON498_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3939533.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14336..14647)
                     /locus_tag="ON498_001272"
     CDS             complement(14336..14647)
                     /locus_tag="ON498_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939534.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14670..15980)
                     /locus_tag="ON498_001273"
     CDS             complement(14670..15980)
                     /locus_tag="ON498_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCR3939535.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16594..17046)
                     /locus_tag="ON498_001274"
     CDS             complement(16594..17046)
                     /locus_tag="ON498_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939536.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17048..17413)
                     /locus_tag="ON498_001275"
     CDS             complement(17048..17413)
                     /locus_tag="ON498_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939537.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17512..21795)
                     /locus_tag="ON498_001276"
     CDS             complement(17512..21795)
                     /locus_tag="ON498_001276"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HCR3939538.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21942..22181)
                     /locus_tag="ON498_001277"
     CDS             complement(21942..22181)
                     /locus_tag="ON498_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939539.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22271..22543)
                     /locus_tag="ON498_001278"
     CDS             complement(22271..22543)
                     /locus_tag="ON498_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939540.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(22626..22877)
                     /locus_tag="ON498_001279"
     CDS             complement(22626..22877)
                     /locus_tag="ON498_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939541.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         6764 a         4992 c         3389 g         7888 t
ORIGIN      
        1 aaccaacaaa catcgacaaa cagtaaatga tataatccat ttccacctcc aagcatattc
       61 aatactggtg aacatgcctt agaaacacga atatagttat ggtttaaata gatattttct
      121 ttaaacattg cttgtttaaa agccatatgc tcttgttcta aacttttcat aaacttttac
      181 tctaatgcac agctagcagc agctgctgca attcctgcta caatatttac cttagctaac
      241 tctttagctg cagcttttcc taattgtttt tctgcaattt ttaataataa atccgcagct
      301 ttttcaaaat taaaagcttt taatgcttca ataaaatctc cctgcgtacc tatagcccaa
      361 gccactcctc ctccaggaag agaattaatg gctacacatc cagcatatcg tttccaatca
      421 actttctcca ttctaggaac aataatatgt tcataaaaga ctcttgctgc tcttccttgt
      481 tcgttatctt gactgattcg ttcacgaata gcaccaaaat cctttatatg gtatacacca
      541 tgttcgtcta aaaaaccaac tgtatcaaaa taaaaagtta attcatctac taatttattc
      601 tttatttcta aatcaactgt attcactttt tctgtggcat acacacttgt tccagtacta
      661 acaaaagtac ttaaacctaa taagaaaaca cctaaaacac caaatatttt aattattctt
      721 ttcataattt ctcctccata aaaaataagt tactttttaa atatcttgtt tatgaattca
      781 ttccccaaag gccttttaag attgatacag cagtccgttc acttctttta gacttgtcat
      841 acaagtcttt taaatgatcc catatcagac tagtttgtct atctagcttc aatccattaa
      901 acgctaatga acggttcatt ttttctatta ctttagataa agctacagaa atttttatac
      961 cttcacaacg cgcatcttgg atttcaataa tagtatcatc aattagtttt tgaatttctt
     1021 gcctatcaac atattcaatc ttagaaagag aatcttttaa agctactaat gattcaacga
     1081 tttctttttt ctcttcagac attttcttcc tccttctagt gacccgtaaa gccatgtaga
     1141 gcagagtcca ttgaaccagt aattacacta tctacaacgc cattccaatt tactctacat
     1201 tttccatttt tcagatttgt cggtccatac ggatagcctt tttgacattc aatgttcgga
     1261 ttataccaga taggattata ccattcagcc gcagaagcag attcagtcga tacaaacaat
     1321 cccacaccaa caaatccaat acacatcaca ctatagccaa ttagtttttt catctcgtat
     1381 ccatctccta aaaaatagaa tagttcttat agttattcat aagacatatt ttttccgttt
     1441 ttgttttatc ctttctgtcc tataacaact tttttcaagt aaagaatgac atagtttttc
     1501 ttaaaaaaac agtctgtttt tttctatttc ttcggtgtat aataaatatg agtgttcatt
     1561 ttttatttta atacgcactc cgtacataat aaatcttgat cgcatcaatc ttttgagctt
     1621 cttgatctac tttcaattga agtaatacaa attgagtaac cttattcatt gtttcttctc
     1681 caaataacaa atattgatcc ggattctcta tatcttgtgt cacctggtaa agtttcccag
     1741 ttgttttcat ttgtacatgc gggtctacat caatactatt gtctttaata caacgatctg
     1801 tcatatagtc cctcacagct tgattgcgtt tataaacatc tgtgaaatta ctccattttc
     1861 tagcaaaatc ttttaagaaa ttttttgtca tttcatctat ttgcatttgt tgcgctgtct
     1921 cgtcttctaa tgaaccaatt tgactgttac tttcagtact ttgaagagtt ccacttgttt
     1981 cttttactgc ttcgattgtt tcttctgtcg ttctcaattg tgttttttgt ttttcagaag
     2041 aaaaccaatg acaaccagta agacctatca aacataacca actaaatact aaaattatcc
     2101 cttttttcac gtttctagtc actgcctttc ttatttcacc ttacgaacca gactacgaat
     2161 ttttgtttga ttgtacgtgc gttgataccg agccataatc cgtccttgct cggcattctg
     2221 ttccacacta gagaaatgtt gtccgccata tgaaacatct gttataattc cggtgtgacc
     2281 atagattcca ggagacaatg caccacctgc ttgccaattc accacatctc ctgcttgtaa
     2341 atcacttggt tttgggttca tgattacttg ccaaccatag gttgtccagt tataatcttc
     2401 ccctattaac atcgcaaaca tgtgtccaga acccattaac tgtgggccac ctaattgaga
     2461 tacataccat gccgtaccac cataacattc cccattattt acggatcgtc caatgatccg
     2521 atccaaaacc ttcgcattgc ctttactttt tcctgcaata agcgaagttt tttttgttgg
     2581 taggctattc caatgcttga tcgcaaaagt atcatattcc gttaagttat aggacgttat
     2641 gatactcatt gctcctcgat aatacggact cccttcggta atacctgctg ccgcatagcc
     2701 tccacgacct agaatcaata aacaacttcc aggatcttta ttgcccacac attgattaaa
     2761 gttgctttgg ctcgccacca taaagccata atagtcataa caagctttta agttcggaaa
     2821 cttcatatag aaaccacctt cagaaccacc aattccacgt gcagaacctt gtgggtaggg
     2881 acaaccagga aaccaggtaa tcccaaaaaa gttcgtatcg tttcttccag attgtgactg
     2941 tccataagct gattcatagc ctaattgaga aagaatcaca ctcggtaata actgatactt
     3001 tacagctgca ccaactgctg cttgcgcatg ttcatctgtg attggaccac gtcctttaat
     3061 ctctgaaaaa atcggtaatc cttcggtata ttccccatta aaagcgacag ctagacctgt
     3121 agcttctcct ccatgctcag aaagttctcc tgaaaaaaag aatggcaata gaaaaaaggg
     3181 aagaataaag aaaagaagac aacctagaca ccctttcttc atttcttcct catcctttct
     3241 tcgataatgg tttttggact gttcgtcctt ttggtaaagt aaaatcagtt cctggttttg
     3301 cgtccaaaat cggcagtgtt ttaggatcct tttctatttt ttccgattgc tctgttggct
     3361 tatctaaaga agaaaatgca ggttgtaacg attttggtgc ggaaatcgac ggtttacttt
     3421 cagacgaacg ttgccccggt tgttgcacag aacgatttcc tgatagagtg gctaccgacg
     3481 aattaggaac agagtattgg ttcaataact gctgcattgc tggataactc ccatttttcg
     3541 cctgattttt cacttggcga atctgttgtg aaagagaacc agcagctgtt aattgtggca
     3601 cgggtacttt tccagaaggc aacggaatcg attgaccaac aaaagcgata cctttattta
     3661 acaatggttt tctttgatta ttcccttgtt gcttactcaa ttgtgccaat gattgtacct
     3721 gttgttttgg tcttactgaa ttcatggacg aatttggaat caaaccattt gaaagaccag
     3781 aattccactc tgttttcgat ttggtagaag acatttcagg ctctacccca tgataacgaa
     3841 catgacgacc actataaggc aaggcttgat ttgaacgccc acctagttca aagggattaa
     3901 ccacttgcct cccttttacc gattcttgtg gtgtacgttt ttgttgaaac ttttgaaatc
     3961 catgtatcgc tgcagaagcg agtggattcg ttgctcgata cacattccct gctaatcctg
     4021 cacctttgat tgccaaattt tgtccagcag ttgatagttg accaggcagt tgtttcattt
     4081 gagacaaaga atgacttgct atgtttttcg tgttgtttat agtttgatct gcagctccta
     4141 accctagttt ctttaataaa tctttccgtt ttacaatgag taaaattgat aggagtgctt
     4201 tgacaaacat atttcctaaa aaaccaaaaa cagtattcat tggaattaaa tcgtctacta
     4261 tgttatagat caaaaatgcc accaaaataa tcacactgta aaaagatttt tgaaacatca
     4321 agcccaaaaa ggactttaag gtattcatga gcatataatc aaatactgga acaaaagaag
     4381 cgagtaatga aaaaggaatc acaagtaata aacacaacac caacatttga aagataaaac
     4441 gaatcatgcc aatcactaca agtggtaatc caatacttag attaagcaat ggcaccatag
     4501 aaccaacgac tgctttataa acagctttag ttggttgaat atagacacga taagccttct
     4561 ttttccctct atcttctcct gcagcagtgg tcacttttgt ctcaatatca gataattctt
     4621 cacttgacgc ttccttggct agaaattctt gtggttcaat ctctgcttga tacaattctt
     4681 ctaaatttgt tgtcccataa tttaacaata tatacggatc aacaacagcc gctttataat
     4741 aaagttggcg cactttttga atggcttcct tttgtgtcgt tggcactaaa acaccattga
     4801 catcttttgt cgccaattca tctagactat ttgtagcaga aaaaataaag ccttctacct
     4861 ccacggacaa atcatttaac cctttggtaa aagcttctcc tttactaggt acgttactac
     4921 catagccaaa ccaaaccatt gaaaaaatga caacgaagag aaatcgaata aacaccattt
     4981 ttgcgtaatg tttccctcta aatatacgaa gagtaaatag atataacaca tacaagacga
     5041 caatccccgc accaatagga tcgtatattt ttgaataaat agtatccgaa aaagtgaaaa
     5101 actggtgaat taactcgttc attgcagctc cttcatagag cttctcaatg acaaaatcaa
     5161 ggccttgcca aatcaattta cttaaactaa agaacatatt cgtcatcatt tttagaaaat
     5221 ctgtgccatt tgccccaaat aacattttgc ctaatgaaaa ttcactatca gataaataag
     5281 aagaaaaggt atcaatatga aatttcccaa tctcatagat cgattgatcc acctgctttt
     5341 ctgccgcata caccaatcca ccaacaaaaa gcagtccgaa tccaatcgtt aaaaaaaaca
     5401 aaacgcacca tttcttttcc ttatgcttta tataagccat tgtttcacct catttctatt
     5461 gcatgattaa cgtatcctgt tcttctactt gagagtgact tgttttttcc gttgtttgta
     5521 atgcagcggt catttcttca aacaaactgt gtacattgag ttttcctaca cgtccatagg
     5581 gatctaacat ccaacactgt cctttaatca tgtggctcat ttcagctaag ttcgcttcat
     5641 ttacttccaa acgtaaatga ttcaaaatct tttctcgctc actgtcttca tcaaaagcaa
     5701 agacgacacc aaaattccct ttttctttat ctgaacctaa gtcaccaata aattgtgtat
     5761 ctaacactaa ttgattcatc tgggaacgcc ccacacgtgc cattgctgca ataatactac
     5821 gtcctgcgtt cgaattatta aatacccatg cttctgtaaa ataaatcgct gttttcttac
     5881 tactatctcg ttttccaaac atttcacaaa aacgacctaa actaatcatc aaacacaaag
     5941 actttcgatc cgcatctgta tatagttctg gtcggacatt gtcatttggt aatttcaaat
     6001 cttggatctc taaaatcgtt gttttggcat taaagtctaa tcccgggttt tctccataag
     6061 aaaatcctaa acgtaaaatc gaatcttcta ccgtcaagcg taaaaactca ccatattctc
     6121 gaacctcttt ttgttccatt tgttctaaat agtcgaccac atgcatcaag ccaacttgtt
     6181 gtccggcttc tttttgaact aaaacttctt ggattccctt taataccgcc ccttttaaaa
     6241 ggtgattggt acctaacgga cttaactcat caatcatatc ctgggctact tgtttcgcat
     6301 ctactcggga taaacaaaca attggatcta atacaccata attacgggga tttgtagcat
     6361 ctaacgtgac ataactcatt ttttgcaaat gttctacaaa caaagggtaa tgtttctgat
     6421 aatacgggtc ttgaatgagt ttcgtaaacc atttgcgctt ttctcctttg ggatccacaa
     6481 ataaactttg tacattcata aacgaactat acaaaaagag caagccaact aaaaaggtct
     6541 tccctttacc ggtttttcca gttactgcta catgaggcgc atcaaacaac gcacctttga
     6601 ttccttccgc aatggctaga agattcaaaa agacaaaacg atttgacgca taaattaatt
     6661 cttctaatgg tgttttttct cctcgagaac caccatcccc taatgtatct tgccgaccaa
     6721 tataccagcc ggattccata cctaattctg atactgctga aaacaaccat tcgccaactg
     6781 cttctacagt tgttcggtgc ttccaatgta actgccgtcc caatggttgc cctaataaac
     6841 atttatagaa caattctcgt tgatcatttt ttgcacggtg gatatggata atcgtatgaa
     6901 gttgttgaat aaccgttgca ctattttgac gaacttcttc aaccgtttga ccgtatacaa
     6961 taaaacatgc gatccaatcg acgacaggtt gcccattatc tactcgttgc tttaaatgtt
     7021 ttactaatcc tcgaacagtt aataagcgtt cactaggttc ttcatcattt tgaaccaatt
     7081 ccttatgctc tgttttgaac cgttccgata gccaattcag ttttaaacca atacctccct
     7141 gtcctttcaa gcgtggatag tgaattttca ttcgcaattc tgctgggaaa gaaagatcct
     7201 gagcaaattg aaatagatca caatttctca tattttcagg tgtttcccca ataggcaaca
     7261 atgctaaata actactccct aactcatctt ctaattgaag aaggccttta tgttccttcg
     7321 gtatacgtac atacgttcct tgattgtctt tcagtacttc ccaattattt tgttcttcta
     7381 ctttgtgagc cattcctcga atatattgca gacgattgag atacaacagc tcttttttta
     7441 ctaagggacg aatagaaaag ctataaagta attgtgtcac ttcttttaca taaggatcac
     7501 tttcagaaaa caaagtttcc aaatcatcta cttctaatcc aaataagcgt gctaaacttc
     7561 ctgctaaatc taaccagatt agatttatta catcatgaaa actctccacc gttgcttttt
     7621 ctttgacttt gattcctaaa ataaatgaag gacgatagac ttgaccttct cgttccaaca
     7681 atgtcactgt ttcttttgca taatacgcac caattgcttg actatcggga gaaaatccct
     7741 ttcctaatgt gtcaaacttc gtccgtaaat tcattgttcg tggatacatc accaactcca
     7801 catcttcata atctgctaaa agcaacagta aacgagccat catttctttt atttcctgtg
     7861 tcttttttgg tgtgtttttt gaaattgttc gtggaaaaac atgatagtag gcccagatac
     7921 aatcctctcc atttaacaaa caatttttct tgaatgcttt tactggttct tttacaagca
     7981 ccaatgaatt tcacctccca ataaaaaagg aacaaccagt tgattgtccc tttttctaat
     8041 cctctaaata aacatactta ttcacaaaaa gtaattcttc caatgcattg tttgatacct
     8101 tgtcccagtt ataatacaca caatcaccat tcatcatacg aatcatataa caaaaccaat
     8161 cccataaata aaaatgcact ttttttccat caggttttag ttttgccagt gctaacacac
     8221 ataaataggg gagtcctata taaaaaacaa ctcctgctgg tggaaaaaaa gaaatgaccc
     8281 aaccaataaa tgaacgtaaa aataaatatt caaagattac gaccaacaaa aaagttgctg
     8341 cattgactaa tgcaactgga aatggaagac gccaattgct tgtaatctgt tgtacgcgat
     8401 aaggttccgc ataaacaatc gaataatcat aaggttccca atccacttca ctatcctcct
     8461 aatccataaa taatttttca atgattttac caaacgcatc aaatacacgt gttggatcat
     8521 ttaagaagaa ataaactgcg gcacctgctg cgatagatat taatgcattt cctacttttg
     8581 atttcataaa atttcgcaat cccagataaa caaccaccat tattaaaaaa tagccacctt
     8641 gcgttaatat aaaatctaca attcccttca gtgtcatttc cgttcttctc ctttcctatc
     8701 caaccaatta tgtgttaatt tttcaacata atactgattt gtttttttcg taatgacaat
     8761 cgtcatttgt tctttttgta aaagtgacgt atcaccatct cgaaagacga ccgttgcttt
     8821 tacaataaaa ggttttttcg tttctttata acgcaatact ttcatctcta cttgatcaac
     8881 tttagctgtt tgattgagtc cttcaggttc tttcatcatg tatgacatat cttttgcatt
     8941 gtttttcgca tacttatcta agaaatcttc cacaaatcct tcaacatctt tcgcttccga
     9001 cgcatcaacc gtttcgtata atgtgtcatc ataagtaatc attcgttgtt tttctgtatt
     9061 ctttggtagt gccgtaaaat aaggataagc aataatacga cagcccttgt ttgaaataga
     9121 aaagggaaga ttgagtactt gtgtgattgt cacacttgtt tctttcttac tctctggatt
     9181 catcgagcga atatcataag ttactttata actagcagtt ggaatggttt caatttcttt
     9241 taatccaata aacgaagcag cagttaattt tcgaacacct gaaacacttc ctggttcttg
     9301 aataaagttt tgtgcataaa actctttttt taatgtttct tgacgttgtt gttcttgttc
     9361 atgattatcg aatgtattca tatacaaagc aacaaaacga ttcataaagg attcaatttc
     9421 aggtgtgtac gttaattctg accgactttg tacttctttc ttttgtaatt gttggatttc
     9481 atctttcaaa aacgacactt gaaccttcgt tttcatggat tgaaaaaatg ccaagccccc
     9541 tgaaaaacaa agaaataaaa ccaaacacca aatccatttc actgcttttt ttgaatggat
     9601 tttcttttgc ttcggtactt tctctgtata aactttttct ttccgccgta gttgtcgttt
     9661 tttcataagc tactccttct agtatgtttt tactagtacg tacgattgac aacgataagc
     9721 ttgtttttct gaaaaatagt ccattcaatt accctcattt tctattcctt ttctctttct
     9781 tttatatcca acaaatccag caatacttgt caacactcct cctagaacag aaagtgcaac
     9841 atgtatttct tcaccagtct ttggtaagac ttttgttttt ccatctgctg ttttgatttc
     9901 aatattgcca cttggaagtt tctttacagg aatcgacaaa tcctttacca aattcccttc
     9961 actatcttgg attaatggaa taccatcttt tacaccaaca attgtttgat cacctgctac
    10021 ttttaatggg gtggttggtg ttggaatatg ggtcaattcc tctacattac tagtgacaat
    10081 cggtacactc attccttgag aagtattcgt cgtaatttca ccgcttgggg ttaccgatac
    10141 tgtgacttct tttgctggaa caataggttt attttctttt ggaattgctt ctgatttttc
    10201 aacaggttgt ttcggttctt ctttttgttc ttcactggta gacgacatcg ttggctgttc
    10261 cgtacttgac gaatcagtat tcttttcaga agaactggtt gttcctgatt gttccggagt
    10321 tgtgactata cttgaatccg ttgttgttga actatgcaca ccttcttctt cgatcgaatc
    10381 agtcggtaaa gttgattcag gaaactctgg ttctggtgtc actggctctt tttcaggttt
    10441 ctctgactgc aattgttcag gttctgctgg tttttccgtt gttcctccat ttagctctgt
    10501 ctcaacatct ggtaatcctt cccctggcaa tgtttctaca atttctgact taggtgtttc
    10561 tacagtttca tctgcataaa ccaatgaact ccctaattga aacgaaaata atgaaattcc
    10621 tagtgttgtt acgactgcta atgttgtttt tttcatgatt attcctccaa ttttttatgc
    10681 aattcgtgtt ttgttcatag acgccgcact aatattatct gaacgataaa agaactcccg
    10741 tccgttttct tctaacccaa catatggatt atttttgaat aattgaatca atagttcact
    10801 ggtgttgtat tttggatagt ttgcaaaaaa ataatactgt gcttgtcgcc catccgaaaa
    10861 ttcaacaagt aattcataac gataccgctt caatcctttt cgttgtttta aaggatctct
    10921 ataccaactt gtccatcgtt ccattcgtgg atagagatag acacgataca catagaacaa
    10981 tgccatacca cataccacca aaaataataa tcgccacata aaaattcccc cttttcttct
    11041 acctttcttg aatcatcgaa cattcatacc ctctatagag atttgaaaaa tccatcatca
    11101 attgttcata taaacgttgt tgcaaatagc gattgtttcg aatacataat tcttcatagc
    11161 catcaatcac ttgatcttct ttttcatagc atcttgtagc tctatgacgc tcagcttgca
    11221 tgacataacc aaactctgca ccatatcgtt ctactgttaa taactgttga ctacgcaata
    11281 atgcaatata acaatcacaa aaatgttcaa ccgacatttg atttttctca cgtttgtgaa
    11341 ataggtattc tttagaaagc acattattcg ttaaacaatc tgataaatag cgataatctg
    11401 gcggtcggtt attttcatct gtgaattttt cccataataa actgattttt attcgttcag
    11461 cggctagata gtcttgttcg cgtggataaa attcatcaat cgcacaaagt tttttatcac
    11521 ctgaataaac aaatccatat aatgcgaaac gaactcttcc ttttcaattg gctctcctaa
    11581 aaattccagt gacataccat cttctccttc ctaaaaaaaa taaaaaagca gaaaccgatt
    11641 tggcttctac ttttctttcc ttttatagct cttttttacg gaagaatccg caaatgacat
    11701 tttttcaaaa aatcaatcgg cacaagttca cgaaaacttg gagcaatttt ggatagaatc
    11761 acttccccat cttgtaaaat acaatcataa aaatcatcag ttacttgata cttacgcttc
    11821 ttacaataat tgataaaata gtagcgatca ttttctattt ccattcgttg ttctactaag
    11881 cgcatacgaa catcatcata gaaataagcc acaaatcgcc cttcaagttc atgtttttca
    11941 atgggatact cttctggtaa caatctattt tttcgtcgtt tatacattgc tggatacctt
    12001 agaagaagtt catctaaacg tctgtcagta tatggtaact gacgaatcaa cgattctaat
    12061 cgttctttat acagatagaa taattggcgc aagcaatagt gtaccgcctc tggattttct
    12121 aatactgttg gatcacgtga ccaatacgca ccaacataaa tcataatttt tttcatttag
    12181 acaatcacac cagcaatact aatgaccgat ccacaatagg tagaacaagt ggcatagcga
    12241 ccaatttcct ctaattctat ctcaatctct tctaaagaaa gatatgtctg ttcttctgtt
    12301 ggctggcgac tcatttccca agtgacagtc accaatgaaa tttcttccat aagaggtttt
    12361 tcgctgctaa gtgctgccac acagttttca tatatcttca ttttatcgca gcctttttta
    12421 accgctaaaa tcgcaggtat tggcaatgaa aatcgataaa agcgaatgaa ttctagcctt
    12481 cctttccttc ggctaaattc caactcttcg agttcttgtt cgtatccctt acgaacttct
    12541 aataagcgtg caatttcttg ccagttctct ttttcctgat aacgtccagc ttttaccgct
    12601 tgttcaatat aaggaactcg aaaacgaaaa taagacaaat caaccatctt ttcatttaat
    12661 gcttcgattg cagcctttaa atccaacagg cgcagccatt ctgttgtcgt atatgcattc
    12721 atgcaatttt ccctcctgac gttcccatcc attctagata gttttgttgc tcgtcattta
    12781 gttcggcatt atcaatccct tcttgtacaa agtctcttcc taaaatacga tctgcttctt
    12841 gtaacaactt taatgtcgga gctacttgtt tcaaaaacca gcgttctgaa cgatctaatg
    12901 agaatttttc tggctttact gtaaaatcca attcttccat cgaacgaaat aattttgccc
    12961 atcgtttatc aacaatccgt gcatcacttt ccagtctatc ccaaacaata agttttgcat
    13021 taattaaacc aacgccaatc tctcctaaac gtttcggtga atcacctaaa ataaatgctt
    13081 ctactgcttg tgttgctttc tcattggcta accgtaactc atatcgatta taaataccat
    13141 atttcgttaa tgcttctaat tctgaaattt tttctcggtt cgcaatttca aaacgtttct
    13201 cataaaaatt aaaaaagaga ggactctttc gcgatccaaa atacaaagat aatccttcac
    13261 ttcgaaaata gccatcatct aaaaaacaac cgccaatatt ctggaatctc ttaaagtcca
    13321 tttctaataa acctgctttt tgtttttcta gtaattcaaa gagatcgaaa tgttcttccg
    13381 tttccaatcc ccaaagttca tctaacgcaa tatcgaatcg gctacattgc acacgtgtat
    13441 aaccagcttc caactttcct tgatttttcc aaatggaata tagccatgtt cgccaatctg
    13501 tttgttgttc acgcatgact tcctcatatt cccggcaacc ttgtcctgaa aattgcacaa
    13561 gtacattttc tgttacagtt cgttttgaat gccagaacac ccagatattc ccacgaacag
    13621 aaagacagtc aaaaagatac aagcccgttg gacgtgtttc aaattctgtc agatccattc
    13681 gtaatacacg ttgaatcact gttttgtagt ctgtcccaaa aaaggaaaga cgcaaataat
    13741 caatcattgc tcgaagatct attccgcttt tcatatacaa tcgaaagcgt tccttgattc
    13801 gttgttcaaa atcctctggg atttttcttt tcccttgttc catctttcta atgtaatctt
    13861 ttgagcagcc catcaattgc gcaaatcttg cttgtgatag cggcttttta tctccactta
    13921 tattatgaat tgcacttaac ttctttctgt agctttttag ctcaatcggt gtcattttct
    13981 cgctcctttt ataaaataac aaaatcgatt ttttttaacc ctttagaaac accgatatat
    14041 caactagtcc ccctcgtttt ttgggaaaag tagtacaact gctgacctta gataatcact
    14101 tgatacaaaa gggtttataa aacttttttc tatgtgttat gttatttctg ccccccatat
    14161 tatatatcgg ggggctttac acaaagaggc cgtgcgcaag cgcacgacct ctttgtgagc
    14221 tgcacacgct gctagcgagt gcgcattata ggcccatgct tcgcagcctt aatgcgcacc
    14281 gccaggcaga gtgcggctac cagactacgt ctagagccct cccgatagat aataactaca
    14341 aaattaaatg atctaatttt gttatttcac tatatttttc aggagaaaaa acgcgataaa
    14401 ttgacaaagt taaatcctct tctaaatcta ctcgataatg atacaatcga gtcaatcgtt
    14461 ctatatcttt tttcgttagc aatacttgat taataacttt tcctgtaaaa acacgttctt
    14521 tgcgcaaaaa gcggtaatca gttacttgtt caatacgatt caaccaatca taaaacgttc
    14581 tggaagaaag agaaggatta atcattcgta ttgctgtagc tactctataa taacaactct
    14641 ctgccattta acttcacctc cttagttgat tacaaaatat gcgcatcaga aaaacttgtt
    14701 ccttttggat cttttgctaa atacatttgg cgctctagtt gttcttgtaa caactgatta
    14761 tcatctccca tcgtttgatc ttgactatcc tcttttactg taaaatcata gacaatcatt
    14821 tcagtaaatt tctcaaattc tttgataaag tgataatctt tgtaaacaaa tggcgcaaaa
    14881 aactctacag gtacatcaaa tcctggaaaa gcatatccac gaccaggttg atctttgttg
    14941 taaaatggct tatttttaaa atcttctcca aaagtcatta aataaccaat gggagacaat
    15001 cgaccaacag aaatacggaa tgttaaatta tcccgaacac cacttggaaa atattctgca
    15061 tctggtcgtt gcattgctaa aataatgtac atccctgctt gacgaccctt taatactaat
    15121 ggctgaatca atttatcgac tctatttctc gcttccatat ccaacgaacc ataaaaagct
    15181 gcccattcat caaaaaagat aaaatgcggt ggtaacccat aataagcata atttttccct
    15241 ggttcatatt taggatcttt catggttttc atataggcat accgttgatt catttctttt
    15301 aatgcatttc gtaaacaaat aatcatttct tcatttattt tgtaaaacac atgtcctttg
    15361 aatacatcga cgtcacctaa tgctgctaaa tctgccgctt ttccatcgca aatatctact
    15421 gtcccaacac ttaaacaagc ttgaattaaa gaatacaata aataggtttt cccaccaccg
    15481 attgcaccag caatcaacat atttggtgat tgatcatata cccaatccaa agttttcatt
    15541 aaatgaatac gtccattttc tgctatacat tgatcaatag gaatccgacc agaatctgaa
    15601 aggaatacat aattgattcg ggctttttct cgttcttcac gaattaaatc acgcaaaaat
    15661 acttcagata acaaatgacc catttttaaa aatcgttcgt gaaaacgccc accatctaaa
    15721 ggaaaagaaa ccactaattc ttgtttcttt tgataaaaat aaacttttgg taatctcatt
    15781 ttttcccttg tttgtttctc actaacttgt ttctttgtat aaacaaaccg attgtcaatt
    15841 aacattcgaa caagtaactg acgccaataa acagaggcaa aatatccttc ttttagtcgc
    15901 gaccaatgca tcaaccctaa aattaaccca ctaaccagca acaaatacaa cacaatactt
    15961 attccaggaa taagatacat ttgccaattc gaaaacgtac ttaaacgtgg ataaaatact
    16021 ttccaactaa accaacctaa cagaatcaaa aatggaaaca aaaagaaacc ataatagcaa
    16081 aaccataaat aacgattgaa atagcgaatt cgccgaccac gatacttgaa aaaatgagct
    16141 ttcaattgtc acattcctcc tttctgtttt tacttttcct gtgctaaaaa cttttgacaa
    16201 aaagcaacta atacaggaat atcacattta gtaaaaaaac ataatcgaaa aagatttaat
    16261 cgatttttat aagggtgtgc acacatatta catattcgta ctatacgaat ccaaatcact
    16321 tcctgatgat cttgtttcca ctggtacggt aatgaaaaac gaacaagatc acgtaaaaac
    16381 atacactgcc caataaccgc acaaatatat gcatttgttt gtacaaaaat cgttggattg
    16441 tctccaaaat actcaatcaa ttgttggata ttttgataac aagcacaatc tctcaaagta
    16501 tttgattggt tttatctgaa ttcaaaatct ccccttcctt ctatcttaga taaaaagaat
    16561 aggaagactc cttcctattc tttcaaaaaa accttatttt ttctcttttt ccttagaatc
    16621 aacacttggt tgtttttgat ctaaaccttt ttcttgtgat tgtttcatat taggattatt
    16681 tgttggagct tctactttct ctaatccttt cgcataaatc actaaatctg aatattcacg
    16741 attgccatct tgtgctggaa tatataccac atgatattct ggatcaatta atttcacagg
    16801 atcacgtcgt ttaaaaccag tcaaatctac atctgctgga aatttaactc gtcgtgcaat
    16861 ctttggttca tctggatttc caaaaactaa ttgtgctcgc tgtgatcgaa tttctcccgt
    16921 ataatcgcct gtttcattat caaatacacg atcagactct tctaatgcgc caaagcgtac
    16981 ggctcctaaa caagtttcat ctaatgcttc tgtaatgtca taattatttg gaaattttaa
    17041 tcccatctta caaaacctcc ccaattttta caccagttaa aaagacacca acatcttgat
    17101 agccagttgt tcgacgctct gtttgtccca tatcatttgt ttcacgacta ttcactcgtt
    17161 ctttacgaaa aacaaaataa gcttcttcaa atgaaatacg cacctctttt tctggtaatc
    17221 gtaatctttt atcctcttca gctgtgagat aaacaaaaaa agcaggctgt tcttctcgcg
    17281 aacactctgc ttgtaattga acatatcgcc cagacacacc attaatttcc tttggtggat
    17341 taggttcccg tgaacccaat aaaaatactt gacctaaaaa ctctttcttc atacgcaatg
    17401 ctttttctgc cataaagttt ttcctcctct taaaaaccaa aataaattct aaatatttct
    17461 acattacaat caaacaagcg gttaaaaatc ctaaccgctt gttcctcatg actaatcttt
    17521 ggctttacgt actttataaa gtgttatacc taaagcaatg actaatacta ctaatccaag
    17581 aaccataagt atactacttg tttgttcacc tgtttttggc aacattggta caacagtagc
    17641 tggtttaacc cgttgttctg gtttcactgg ttgcggtggt tttggcgctt ccacaatttg
    17701 aaccgtttga tctttgtttg ttagatctac attgtgtttc gccacttctt caaacgtttc
    17761 tttctcatct tccgttttcg cttggagcca ttccgcaaaa ctcaagcctt ttcctgcaag
    17821 attgcgaccg tccactaaga attcaaataa gaattccatc atttctttat cggccgcaaa
    17881 gactttttcc gcatgagtgc cttcaaatgg tgtgccgtct ggtgttaagt agccttgaac
    17941 aattaagcga tagatttgac caatcgcaag accgtctact ttgacccatt caagcacagt
    18001 ttgatttggc aaggccaaga tctcttttcc gccattttta ttcgtcgctt tcgtcgtaat
    18061 ggttggatta ctgattttga tggtttggtc attgtcggta atatctgcgt gagaagccaa
    18121 taatacgcct tctctaaaca gatcttcaaa gaccacgact tctttgcctc gcaacgcacg
    18181 tgcgtctaga ataaagtcta gattgactgt accatccgtt gtttctgcca caaacgttaa
    18241 ggtacttgtg acttcttttc catctactag cactggtttt tccgttactt tgtccattag
    18301 aacaccgttg actgtgtatg tttttccaac gaccaaatcg tggtatttca cagcgtctgt
    18361 aatggtgact ttcgttaatg gattaacctc tttcaagcca gtgggtttat gtgtagccgt
    18421 tgtacgtaca cttgggttag ttactcgtac cgtttgccct ttgtcgttga tgtctttatg
    18481 gtttgctagt tcttcatcat ttctaaaaag actttcaaac acgacgattt cacgaccatt
    18541 tatacctgtc aaatcaaagg taaatggtac gatcaccgtt tcattttctg ctttggcaat
    18601 aaagccaaca ctcgtttcta ccgtatttcc atgcacaagc aagggttcac cggttgcttt
    18661 atccattaag atccctttga ccaagtatgc ttttcctgga agaatctcgg taatttcaac
    18721 ggtatcgttt aaggtaaaca atcctgttgg gtctgcgact gtcaatccgt cttcaaagac
    18781 agccgtggta tgaacggttg gattattcac tttcacactt tgggcttcat cgttgatatc
    18841 cgcatgtgct gcaacttcaa tgccatcacg gaaaagtttt tcaaaaacaa cgatcgtttg
    18901 acctttcaga acactagcat taaccacaaa ttcaaggtct tttgtgccat tgactttgtc
    18961 tgcaataaag gccaaggtac ttgtgatttc tttgccatct tgtaaaattg gcgcattcgt
    19021 tgccttgttc atcaatactc ctgaaatggt gtattctttt ccgacaaaca agttggtata
    19081 agaaacactg tctttaatga cgatcgtttc taatggatca accacttttt caccatcggc
    19141 tttatttgac gcttgtgttt ctagttttgg tggaatcaag taattacgta cagaaaccac
    19201 taacgtttcc ccatcatctg gcgcattttg tttgaatgca accgtgaatg cttgtttatc
    19261 tgtatttggt tggaaacttt ctaatggggc attttccaag aagtaatagt catcggcaac
    19321 tagacctttc actaaaattt ccccgttttg atcagtggta tattcaccaa ttgcttgatt
    19381 tttaccgtta tacaaggtaa agttcacacc tgcgagcggc gttttcttct tgttccattc
    19441 ttcatacttg aataatttta cacttttccg tgcttgacgg ttcgcaaaat ggatcacttt
    19501 gatcggttct gtatcattgg taatcgagaa cactaatggg ttttgattca aaacgtaatt
    19561 tacgggggct ttgatttctt ccaaacgata gcgatctttt ccataagtaa gtgtaccgtt
    19621 agttaggaaa aagccttttt cattggtttt aaagatatcc gttgtttctg tatcatttgg
    19681 aatctccatt gtgacataac gattggctaa agtatcaaaa atcttgaatt ctgtattagc
    19741 aagtggaatc acttttcctg tttcttgatc cactttcacc actttaagac gttgttcaat
    19801 tactttatct tctaaaacat aatggaaagt ttgcccattt tcagaaattg tgacttcaag
    19861 tggttcaaat agtttatacc cttcttttcc tttggtttca gaaagaatgt acgtatcaaa
    19921 gacaacggta tttttaccaa aagtagcata accatcattg tctgtcacaa cggttttcac
    19981 cactttgcca gttgtttttg acgtcaaagt gatttcaatg ccagctaatt ttggtttgtt
    20041 atctggattc gttgaatcga ctaaatcttt attagcaact ttcactaaat caatggttcc
    20101 tttgatcact tgatcttttg cggtcgcatt gacagttttt ccaggttcaa caacaatttc
    20161 aattgcttca tcagaaagca cataaccttt tggcgctttg atttctttta aaacatattt
    20221 gccaggatca agatctttaa tttctgcctg tactttgccg ttaatttttt tcaaggtaat
    20281 ttctttaatc tttttcccat ctgcttgata caaaccatag accgcatttt ctaaagaagc
    20341 tgcaccttgt gctttcacac cagtttccgc atcttcttta aacacacgaa tgtttccttg
    20401 ttttagaatt tgtaaattaa taacagacgc attaggttct gttggatgta acacaccaat
    20461 tgtttgagct cctggacgac gataggctaa tggcgcacct tgaactttaa ttttattcaa
    20521 ttgaattttt gctttatccg gtgcctcttt tgtgactgta atccatactt cattgccgtg
    20581 aatagataca ttgacacctt ttggtttttg aatgtctttc acatatttca aaaaatcatt
    20641 tttattcttt actttcaacg tttcgccaat ttttaccgta tgtgtttgat tattaaaagt
    20701 tgctttttca ccaaagttat taatcaaacg ttgaatttct gcttttcgac gatcataatc
    20761 aacacggata tttttgattg ggatttttgt gcccaaagct tcccaaatca taaattgtgc
    20821 cgcaatgtat tgttcatcgg atttgtcttt atttgtgaca tatcctaaat aagagatcaa
    20881 gcttgttttt ttccgtacat ttgaattttt caaatagttt ttcaaagcag tagctgtata
    20941 aacttgacct tctgcggctg agacaccagg ctcaatacaa aacgccactt gtccattaat
    21001 ctcaaaacga accatataat ttaaagtgaa gtttagattg ttcggactag aagtaacacc
    21061 aatattttcg ccccaatcaa ttttccgacc acctaaaaca gctgcattgg ttgcacggcg
    21121 aacacgaacc gaacgattgg ttggtactgt tactaacagt gtaggtgtac ctgtttttgg
    21181 aatgttctgc tcttttttct tttgattttc aatactagcc gtagattctt tgtttggttt
    21241 cgttggttga ttttgactag ctgcggttga agagattgtt ttttcaacgt tttcctcatt
    21301 ttttccttct ttgtcagcag tagccgctgg ttgttccact gtttcttcta ttcgtttctt
    21361 ttcttccgct tgtttctcag aagactctgg ctgcacagaa ggcgttgctt cggcagcttt
    21421 ttctccttct agagtggacg tttctgaagc aggggttgta attgaggcag tttcttcaac
    21481 tggttgtgaa gtaccaggac tattccctgg gtgttgtgtt tccccaatcg tctgatcgag
    21541 agtcgttttg gaattgaccg ttggcgcttc tgtcacaggg gcctgttcaa cagtggcttc
    21601 accagctggg accccggctt ctgctggttg gacttctgtc acttcagaag cgacttgggc
    21661 aatggcttcc atcgtaggtt gtatagccgt cgtttcttct gcatgtgctt gagcagttcc
    21721 caccaagaaa cagaattgag aaaaagccac caatgcgagt gctttcaacc aattcatttt
    21781 cgtttttgca tacacaagaa ttcctccttt tcctgagaat tttatataat aaaacaaaac
    21841 gccgttacca aaagtgataa cgacgtgaac tgtctcttct atttttatct ggcgctctta
    21901 tctgaacaca tttcatcaat gattttaccc tcccactata attacagaac aaaaaaagaa
    21961 cctgcttcgt ggaaatcaac tgtccatttc ccaattggaa taccagcttt tttggcaact
    22021 tcaccatcac caaatctcat gccataccaa ttactacctt catccttaat ttctttccaa
    22081 ccatatgaca tagcttcatc taatgaatca aaaatcatga cactattgcc aattgttaca
    22141 ggatctggct taagatcttc tggaagagaa atatcaatca taccataatg atccgattcc
    22201 tcagtattat tgtcattact agctactggt ttggttgttc tactggctta ttttgataca
    22261 acaagtactc ttattcttct tttacttctc cgttttcaat ttcccattca aaccactccg
    22321 caaagtcttg cttaaaacca tctactcgat acctattttg taaataggca tcttctgacg
    22381 tcgtaggatc taaataggta ggtttattct ctggacgagc atagacacct acccatgttt
    22441 cgtaatctcc ttcaaaataa ctatctacca aatagccttt ttttctaaga atctcccgaa
    22501 cttgattttt aattattgat acttgttcaa aaccatcact cattctatca cctcaaataa
    22561 ctctagattc tcataaatat tcccgacaat ttcaatccta tcaatgatct ctaacaaatc
    22621 gctacttatg cttccccatt cgtataagaa tttcccttcc gcaaaataaa ttttccataa
    22681 acttcttcat gatcgtccca accaatatca ctctcgaaaa attccacgtt gttcttatct
    22741 tttagctcag taaatgccat aagttcaaca tgttttccaa cctcgaaaca atcacaccac
    22801 ccatcaaccg ttacacgtgt gccttcaata cctaagaaac tttgtcctcg tgtattaaac
    22861 actaaaactt tccgcatttt ttgctcatta attaatcacg cgcgaaatct aggaatcaca
    22921 acttattcgc ttcttcttta taaataaaaa aactccaata atcggaaatt actatctttt
    22981 cctcattcct tttttaaatc ttcatacttc ttcttatctt tgtcctaacc taa
//
LOCUS       DAJSVD010000037        22986 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSVD010000037 DAJSVD010000000
VERSION     DAJSVD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.037"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(105..560)
                     /locus_tag="ON498_001280"
     CDS             complement(105..560)
                     /locus_tag="ON498_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939542.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="ON498_001281"
     CDS             complement(714..1922)
                     /locus_tag="ON498_001281"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR3939543.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="ON498_001282"
     CDS             complement(2034..2366)
                     /locus_tag="ON498_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3939544.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="ON498_001283"
     CDS             2514..3389
                     /locus_tag="ON498_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3939545.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="ON498_001284"
     CDS             complement(3471..4052)
                     /locus_tag="ON498_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR3939546.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="ON498_001285"
     CDS             complement(4058..5317)
                     /locus_tag="ON498_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3939547.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="ON498_001286"
     CDS             complement(5319..6365)
                     /locus_tag="ON498_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939548.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6600..6875)
                     /locus_tag="ON498_001287"
     CDS             complement(6600..6875)
                     /locus_tag="ON498_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR3939549.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7118..8428)
                     /gene="der"
                     /locus_tag="ON498_001288"
     CDS             complement(7118..8428)
                     /gene="der"
                     /locus_tag="ON498_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3939550.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="ON498_001289"
     CDS             complement(8610..9839)
                     /gene="rpsA"
                     /locus_tag="ON498_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR3939551.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9974..10654)
                     /locus_tag="ON498_001290"
     CDS             complement(9974..10654)
                     /locus_tag="ON498_001290"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3939552.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10703..11338)
                     /locus_tag="ON498_001291"
     CDS             complement(10703..11338)
                     /locus_tag="ON498_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3939553.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11404..12807)
                     /locus_tag="ON498_001292"
     CDS             complement(11404..12807)
                     /locus_tag="ON498_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3939554.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12794..13825)
                     /locus_tag="ON498_001293"
     CDS             complement(12794..13825)
                     /locus_tag="ON498_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939555.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13856..14077
                     /locus_tag="ON498_001294"
     CDS             13856..14077
                     /locus_tag="ON498_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR3939556.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14242..14979)
                     /locus_tag="ON498_001295"
     CDS             complement(14242..14979)
                     /locus_tag="ON498_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR3939557.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15132..16472)
                     /locus_tag="ON498_001296"
     CDS             complement(15132..16472)
                     /locus_tag="ON498_001296"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR3939558.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16709..18232)
                     /locus_tag="ON498_001297"
     CDS             complement(16709..18232)
                     /locus_tag="ON498_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR3939559.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18610..19221)
                     /locus_tag="ON498_001298"
     CDS             complement(18610..19221)
                     /locus_tag="ON498_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3939560.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19344..19469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19551..20267)
                     /locus_tag="ON498_001299"
     CDS             complement(19551..20267)
                     /locus_tag="ON498_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3939561.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="ON498_001300"
     CDS             complement(20273..20842)
                     /gene="scpB"
                     /locus_tag="ON498_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR3939562.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20826..21623)
                     /locus_tag="ON498_001301"
     CDS             complement(20826..21623)
                     /locus_tag="ON498_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR3939563.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21593..21964)
                     /locus_tag="ON498_001302"
     CDS             complement(21593..21964)
                     /locus_tag="ON498_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3939564.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="ON498_001303"
     CDS             complement(21983..22870)
                     /gene="xerD"
                     /locus_tag="ON498_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3939565.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6442 a         4913 c         3900 g         7731 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     6481 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     6541 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     6601 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     6661 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     6721 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     6781 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     6841 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     6901 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     6961 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     7021 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     7081 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     7141 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     7201 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     7261 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     7321 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     7381 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     7441 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     7501 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     7561 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     7621 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     7681 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     7741 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     7801 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     7861 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     7921 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     7981 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     8041 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     8101 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     8161 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     8221 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     8281 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     8341 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     8401 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     8461 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     8521 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     8581 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     8641 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     8701 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     8761 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     8821 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     8881 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     8941 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     9001 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     9061 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     9121 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     9181 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     9241 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     9301 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     9361 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     9421 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     9481 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     9541 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     9601 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     9661 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     9721 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     9781 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     9841 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     9901 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     9961 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    10021 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    10081 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    10141 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    10201 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    10261 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    10321 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    10381 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    10441 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    10501 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    10561 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    10621 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    10681 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    10741 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    10801 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    10861 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    10921 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    10981 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    11041 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    11101 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    11161 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    11221 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    11281 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    11341 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    11401 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    11461 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    11521 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    11581 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    11641 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    11701 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    11761 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    11821 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    11881 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    11941 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    12001 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    12061 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    12121 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    12181 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    12241 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    12301 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    12361 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    12421 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    12481 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    12541 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    12601 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    12661 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    12721 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    12781 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    12841 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    12901 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    12961 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    13021 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    13081 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    13141 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    13201 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    13261 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    13321 aaaacagctg caccaaactc tcatacgcag tatcaattaa aatttcacgt ggcaattggc
    13381 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    13441 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    13501 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    13561 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    13621 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    13681 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    13741 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    13801 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    13861 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    13921 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    13981 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    14041 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    14101 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    14161 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    14221 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    14281 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    14341 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    14401 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    14461 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    14521 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    14581 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    14641 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    14701 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    14761 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    14821 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    14881 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    14941 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    15001 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    15061 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    15121 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    15181 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    15241 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    15301 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    15361 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    15421 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    15481 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    15541 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    15601 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    15661 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    15721 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    15781 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    15841 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    15901 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    15961 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    16021 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    16081 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    16141 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    16201 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    16261 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    16321 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    16381 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    16441 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    16501 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    16561 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    16621 agctattgac ttgtccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    16681 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    16741 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    16801 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    16861 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    16921 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    16981 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    17041 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    17101 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    17161 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    17221 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    17281 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    17341 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    17401 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    17461 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    17521 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    17581 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    17641 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    17701 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    17761 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    17821 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    17881 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    17941 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    18001 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    18061 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    18121 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    18181 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    18241 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    18301 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    18361 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    18421 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    18481 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    18541 tacattattg catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct
    18601 ttttgatatt tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa
    18661 ccatggaaga agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat
    18721 caagttgaaa ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg
    18781 tgttgcgttc agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat
    18841 actcataaaa atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc
    18901 tttatgctgt ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga
    18961 taagaaacta gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg
    19021 taatactgaa cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct
    19081 gatcaatacc gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg
    19141 aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt
    19201 ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt
    19261 ttgacgtttc tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat
    19321 ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg
    19381 gaatttcacc gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg
    19441 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    19501 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    19561 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg
    19621 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    19681 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    19741 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    19801 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    19861 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    19921 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    19981 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    20041 gcaacagcag aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga
    20101 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    20161 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    20221 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc
    20281 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca
    20341 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    20401 cagttgttcc atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca
    20461 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    20521 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt
    20581 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    20641 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    20701 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    20761 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    20821 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    20881 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    20941 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    21001 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    21061 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    21121 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    21181 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    21241 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    21301 aattcttctc gaggatcttc ttcccataat tcttcatcgt ctgtgccgaa ttcttggaca
    21361 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    21421 gcaacagcta gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    21481 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    21541 tcgagaggac cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    21601 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    21661 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    21721 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    21781 aaataccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    21841 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    21901 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    21961 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    22021 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    22081 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    22141 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat
    22201 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    22261 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    22321 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    22381 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    22441 cagtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    22501 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    22561 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    22621 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    22681 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    22741 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    22801 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    22861 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    22921 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    22981 tctttt
//
LOCUS       DAJSVD010000038        22944 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSVD010000038 DAJSVD010000000
VERSION     DAJSVD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.038"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..193)
                     /locus_tag="ON498_001304"
     CDS             complement(<1..193)
                     /locus_tag="ON498_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3939566.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNI"
     gene            393..659
                     /locus_tag="ON498_001305"
     CDS             393..659
                     /locus_tag="ON498_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3939567.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            672..830
                     /locus_tag="ON498_001306"
     CDS             672..830
                     /locus_tag="ON498_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939568.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(827..1057)
                     /locus_tag="ON498_001307"
     CDS             complement(827..1057)
                     /locus_tag="ON498_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3939569.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            1129..1284
                     /locus_tag="ON498_001308"
     CDS             1129..1284
                     /locus_tag="ON498_001308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939570.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            1441..1596
                     /locus_tag="ON498_001309"
     CDS             1441..1596
                     /locus_tag="ON498_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939571.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1593..1817)
                     /locus_tag="ON498_001310"
     CDS             complement(1593..1817)
                     /locus_tag="ON498_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3939572.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2084..2419
                     /locus_tag="ON498_001311"
     CDS             2084..2419
                     /locus_tag="ON498_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939573.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2652..3593
                     /locus_tag="ON498_001312"
     CDS             2652..3593
                     /locus_tag="ON498_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HCR3939574.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            3595..4485
                     /locus_tag="ON498_001313"
     CDS             3595..4485
                     /locus_tag="ON498_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCR3939575.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4528..5328
                     /locus_tag="ON498_001314"
     CDS             4528..5328
                     /locus_tag="ON498_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3939576.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5343..6194
                     /locus_tag="ON498_001315"
     CDS             5343..6194
                     /locus_tag="ON498_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3939577.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6191..6508
                     /locus_tag="ON498_001316"
     CDS             6191..6508
                     /locus_tag="ON498_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939578.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            6501..6683
                     /locus_tag="ON498_001317"
     CDS             6501..6683
                     /locus_tag="ON498_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939579.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            6685..7155
                     /locus_tag="ON498_001318"
     CDS             6685..7155
                     /locus_tag="ON498_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939580.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            7152..7694
                     /locus_tag="ON498_001319"
     CDS             7152..7694
                     /locus_tag="ON498_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR3939581.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            7691..8074
                     /locus_tag="ON498_001320"
     CDS             7691..8074
                     /locus_tag="ON498_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939582.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            8071..8301
                     /locus_tag="ON498_001321"
     CDS             8071..8301
                     /locus_tag="ON498_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939583.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            8308..8511
                     /locus_tag="ON498_001322"
     CDS             8308..8511
                     /locus_tag="ON498_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCR3939584.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8508..8804
                     /locus_tag="ON498_001323"
     CDS             8508..8804
                     /locus_tag="ON498_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939585.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            8881..9294
                     /locus_tag="ON498_001324"
     CDS             8881..9294
                     /locus_tag="ON498_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3939586.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            9307..9693
                     /locus_tag="ON498_001325"
     CDS             9307..9693
                     /locus_tag="ON498_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939587.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            10185..10257
                     /locus_tag="ON498_001326"
     tRNA            10185..10257
                     /locus_tag="ON498_001326"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10218..10220,aa:Lys,seq:ttt)
     gene            10643..10903
                     /locus_tag="ON498_001327"
     CDS             10643..10903
                     /locus_tag="ON498_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939588.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            10946..11119
                     /locus_tag="ON498_001328"
     CDS             10946..11119
                     /locus_tag="ON498_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939589.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            11508..12341
                     /locus_tag="ON498_001329"
     CDS             11508..12341
                     /locus_tag="ON498_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HCR3939590.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12334..13749
                     /locus_tag="ON498_001330"
     CDS             12334..13749
                     /locus_tag="ON498_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR3939591.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13761..15290
                     /locus_tag="ON498_001331"
     CDS             13761..15290
                     /locus_tag="ON498_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3939592.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15208..16260
                     /locus_tag="ON498_001332"
     CDS             15208..16260
                     /locus_tag="ON498_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR3939593.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16262..16579
                     /locus_tag="ON498_001333"
     CDS             16262..16579
                     /locus_tag="ON498_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939594.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16706..17329
                     /locus_tag="ON498_001334"
     CDS             16706..17329
                     /locus_tag="ON498_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HCR3939595.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17344..18234
                     /locus_tag="ON498_001335"
     CDS             17344..18234
                     /locus_tag="ON498_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HCR3939596.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18257..18472
                     /locus_tag="ON498_001336"
     CDS             18257..18472
                     /locus_tag="ON498_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939597.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18484..18825
                     /locus_tag="ON498_001337"
     CDS             18484..18825
                     /locus_tag="ON498_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939598.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18825..19196
                     /locus_tag="ON498_001338"
     CDS             18825..19196
                     /locus_tag="ON498_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939599.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19189..19584
                     /locus_tag="ON498_001339"
     CDS             19189..19584
                     /locus_tag="ON498_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR3939600.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19586..19963
                     /locus_tag="ON498_001340"
     CDS             19586..19963
                     /locus_tag="ON498_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939601.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19964..20572
                     /locus_tag="ON498_001341"
     CDS             19964..20572
                     /locus_tag="ON498_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3939602.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20572..20913
                     /locus_tag="ON498_001342"
     CDS             20572..20913
                     /locus_tag="ON498_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939603.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21155..>22944
                     /locus_tag="ON498_001343"
     CDS             21155..>22944
                     /locus_tag="ON498_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR3939604.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIK"
BASE COUNT         8274 a         3361 c         4899 g         6410 t
ORIGIN      
        1 caatattcat caaatcatct atatcaacat tgaatatctt agctatctca tttaaggttt
       61 ttaattttgg tgtatatttt cctttttccc attcgcttat tgaggatgaa ctttttcttc
      121 ctagtttttc ggctagttct tgttgatcaa tattatactt ttctctaaga aactttagat
      181 tttcagcgaa catagtaaca cctccttgag ttaattgtaa caccgtttcg gaaaaaatgt
      241 aacaggtttc ttagaaaata ttttagtgtt tttaaagaag tatttacgca aaatagaaat
      301 cgatttcagg aaaactgaaa tatgcacttg acttcggaaa aaccgaagta tataatttag
      361 tcagaaagtt aaagagagga ggcgaataaa atatggatac cgcgaagaaa gaaaaatata
      421 cattgagaca attaagagct ttaaagggtt attcaaaaga agaactgtca aggaaaagca
      481 aagtgacatc aagaacaata tttatttacg aaaacgatgt agataaaatg aggaacggaa
      541 agtacgccac tctagaaaaa attgctaagg cattgggtgt tagagtttcc gatatttttt
      601 tagaccctga ttcggaaaaa ccgaaatcaa taacaggtga aacaaaacaa gtcagctagg
      661 aggtgtgaat agtggaagag aagaatataa aagtaccgac tcttgaagaa tatacaaaaa
      721 ttgttgtcga aacagacgag cagaacccga aaacaatcgc agttattacg gcgaatgata
      781 ttgaatcatg cgaaggtttt cgagttcgaa tgacccctag atatgattaa tgttctttat
      841 cacgtggagg tataggatcg tttccaaaac tatcttttga ttgtatttgt ccattctttt
      901 tttggacaat aaactctgat tgctgttttt tagaaatctt tttaccatac tcaacagcat
      961 ctgatttatt atcaaatagt tttgtatctt cggaattacc ttcaccacgt acagcccatt
     1021 tgccatttct aggagatacc cattgatttt tacccataac aattcacctc gctttcaagt
     1081 taattctatt aagaggtgaa aagaaaacaa actgatggag gtgagattgt gagtaacaag
     1141 ccaaaaatca ctttgaatgg cacacctaat cttcaaagat tagcagatga aatggctaga
     1201 atacgttcta gacattgggg agttactgtg gtggcaacca taattgaaca aaaagaaaag
     1261 cccacacagc cggcaagcaa gtagtgggca ccatagaaca ataatattat atcacacact
     1321 cgccacttcc gaaaagagga gttgctgctc tcctttcaag tttgccaaag tccacgtttc
     1381 ttcattttgg caactcctct tcttggaggt ggtgacggcg agagacgaag gagggatgac
     1441 atgaaggaag aatcaaaaat agggattttg aatgagaaca attacacaag attaattatt
     1501 gaaacagaag aaggggaaaa agtagcagaa gttacattga ccgatgcaac tcctgctgac
     1561 ggttatcgta ttcgattaac tcctaaatat gattaacctt ttggtgggtg tggatcgttt
     1621 ccgtgactat cacggctttg aatttttcca tctctgccat gaataaatag ctcagatcct
     1681 tgatttttag aaatctctct agcgattttt actgcttctg attttgtatt agtatgtgca
     1741 gtcgctttag aatttccagc acctttgaca ttccaaccac cattggaatt aggtacaaca
     1801 tgttgatttt ttcccatagt tttcacctcc atttctatag aaatatttta tcaaatagaa
     1861 atggagaaat tcaataaacg aaaggaggga tcaaatgaca ttacaagagc atcaacactt
     1921 gatgcgaaaa ctaaatcagg aataccacga ggaaatgaaa cctagattgc taggggaaca
     1981 atggcgtgaa cgccagaaga agtggttaga aataaaaaag tgactcagcc gtccaaagca
     2041 atgagtcaca aaccaaaaat tatacctaag gagattttat cacatgaata agaaaattga
     2101 aaatcttatc gaagaattga aacgtgaatg ccaaaagcaa ggtgtttcta ttatttgtac
     2161 agcccaaaaa gaaggggaac ttaaaagtct cgtttatggt gagacaacag aaattttact
     2221 ttgtcttgct atgcaagaag aacatttaga tgaaaatctc ccattgtcag cacatattat
     2281 gcgtagaatt gctgtggatg cttacgaaca agcgaaaaat gaagaagaaa atcaaccttc
     2341 aaatcatact tttgttatta ataacaagga agatttagct gatgtgatga ctcgtattct
     2401 caagggggaa tttcaatgat gaatgaaaaa acaattgatc tactcattat tttatttttc
     2461 acgttagtga ttgtagcgaa aagttatgta atctttggct tgttattcgc aattaccatg
     2521 cttgtgcttt gcgtgttagc aagcagaaaa gaggttaaac atgaacgagt tagcaaatct
     2581 tgacaactat ttaactgatc ctgattatgc taaaccacca tatgaagcgc caattgatga
     2641 ggaggatgaa gatgagtaaa tctaccttag aaatgagcca tcaagaatgg ctcgaagacc
     2701 gcaaaagagg tattggtggc tcagatgttg caaccgtact tggattaaac aaatacaaat
     2761 ctccttatca attgtggctt gagaaaacgg gtcaaattga attgaaagat ttagaaagcg
     2821 aaccagctta ttggggtaat gttttagaag aagtggttgc taaagagttt caggaacgta
     2881 caggcaaaaa agtacgcaga agaaaccaag tatttgaaca tccattacat ccatttttaa
     2941 gagcaaatat tgatcgggaa gtagtgggag aaaatgccat tctggaatgt aaaacagcca
     3001 atcaatttct cggaaaagag tgggaaggtg aagaggtacc gcttagctat ctctgtcaag
     3061 ttcagcatta catgaacgtt ttaaacaaag actattgtta tatcgctgtt ttgatcggtg
     3121 gacaaaaatt catttggaag cggattgaac gagatcaaga gctgatcgat acaatcactg
     3181 aacaattagt agagttttgg gaaacgaacg ttcttggagg tatcgagcct gtaattgacg
     3241 gaagtggagc gactgctgac ttcttaaaag aaaaatatgc agatgtagaa gaaaatcaaa
     3301 cagctctacc atcacgtttt gatgaactta tcgagcaaaa aagagaactc aagcggacga
     3361 aaaaagaaat tgaatcatct attcgtcaag tagacaacga gatcatcagt gagctaggta
     3421 aacgtgaagc cagtatcggt atcacacaaa gaaacattat cagctggaaa cttgttcgta
     3481 caagacggat gaacacgaag aaactagcag agaaatatcc agatgtcgca aatgatgaag
     3541 agatttataa cgttactgaa tcaagaaggc taaccgaaaa ggagatcaaa taatatggca
     3601 acaaatgaat cgttaaaaaa tcaattggca gaaaagccac agaaacaagt tgcaccagga
     3661 cagttagggc ttaaagctct aatgaataca ccaacaatga gaaagaaatt tgaagaagta
     3721 cttcatgaca atgctaatgc ttttatgtcg aatgttatga ctcttgtatc taatgacagt
     3781 tatcttgcag atagtgaacc gatgtctatc atgagtggtg cgttaactgc tgcaacatta
     3841 aatcttgggc tagataagaa tttaggttat gcatatttag ttccattcaa tagtaaaaac
     3901 aagcaaacag gaaaatggga aaagaaagct caattcatgc ttggctataa aggatatatc
     3961 caattagccc aacgatcagg taaatacaaa gcattaaatg tgatcgaagt ttacgaagga
     4021 gaactaaaaa gctggaaccg actgacagaa gagtttgagt ttgatccaaa tggtagaacg
     4081 tctgatgaag tcattggata tgttggctat ttcgagttac tgaatggatt caaaaaaact
     4141 gtctattgga ccaaacaaga aatcgaagct catcggattg ctaacaataa agatcgcgat
     4201 aagacaaagt taagtggtgt gtgggcatct gattacaatg caatggcacg aaaaactgtt
     4261 ttgagaaatc ttctttctaa atggggaatc ttgtccatcg aaatgcaaga agccaccaca
     4321 tcggatgaga gagtccaaag ggttcaagaa gacggcagca ttattgctga aacagaagtt
     4381 gaagaagata ttcctgaaag aaaagaagca gaggttatat ctgaagaaaa tgaagatgta
     4441 caaactggat tatttgatgc atctaatccg ccgttaaaca aataatgagg gagctttctc
     4501 ccttgctttt ctagaggaga aatacgaatg aacacaggat atgtcaaatt acatcgaaaa
     4561 gtgatgaatt cattcgtttg gaccaatcct tatatgtata aattatggag cttgtgtttg
     4621 atgaaagcga gtcatgagaa tcgcaagata ctttttaatg gaaaagagat acaagtgaac
     4681 agcggagaat tcgtcacagg gcgcgatgct atcacatctg agatgaacga aggcgtgaaa
     4741 cgtgaacatc aagtgaacag cggttctgta tggagatggc taaaacaatt tgaaaaaaac
     4801 ggaatgttga acatcaaatc aactacgaaa tacagcgtta tatcaataaa aaactggtct
     4861 ttgtaccaaa gcagtgaaca acaaatgaac atcaaacgca caacaagtga acaacaagtg
     4921 cacacaaaca agaatgaaaa gaattataag aatgaaaaga ataataaaac atcatcgtcg
     4981 caaccacgca aaaagcgtgt ttacgacacc gactcagttt actacattct cgcggaggag
     5041 ttattcaaac agatttgtca gaatcaggaa atcaaaaagc cgaatctgca aagttgggca
     5101 gataacattc ggaaaatgat cgagatcgac aagcggaccg aaaaccaggt acggggaatg
     5161 attgaatgga gtcagcacaa tgtgttttgg gcatcgaata ttttatctgc taaaaaattg
     5221 cgagaaaaat acgacacaat ggcagcgcaa gcaaatcgtg attataaaac aaaacaaact
     5281 aaaacgcttg aatacgagaa atttagcaca gatgagttgc ctatttgaga ggaggcgtag
     5341 gcatggaaac tgttggggaa atcatggaaa agctgataca gaaagtgctt gtccaacgtg
     5401 gcgaatgtcc tgaatgcgga cagcctttgt atggatggcg tacgaagaac cctgatggct
     5461 cagaacgttg taagccaacc tgtatgcaat gtgggtataa agcattgcga gttcaggaag
     5521 acttacaaac agaacgaatt tacaacgaga gcctgaaagc aagagcaatc aattttttca
     5581 aaggtggttc tgttgtgcct aatcaagcgt tgtttgattg cacattgcag aattatcaaa
     5641 ttgtcgatca agaaacaaga caagcggttg aagtaaccaa acgctttgtt aattcagtct
     5701 tgttaggaaa tccaagtcac cttgttttaa ctggaaaaca aggaacaggt aaaagtcacc
     5761 tagcgatggc agcggcttgg gaagtcttga agcgatcaaa ctacgataaa aaaatcttat
     5821 ttatcgggtt acaggaaatg ctggatcaaa tcaaattttc gtacaacaat cctgaactca
     5881 gaaaaacgat tgagggatcg ttgattgcag atatcaaaac agcggattta gtcatcattg
     5941 atgatatcgg ttcagaacta ggaaaagatg catcagatag tcgagcgttt ggcataaaca
     6001 cgctaaattc gttcttggat gcacgacaga acctagcaac gattatcaca acgaacttgc
     6061 ttggtgaaga actgaaaaaa gcttacggta cgagaacaat atcaagaatg tttgtcaact
     6121 ctgatggatt tacgatggta ttttctcaaa cagcagacaa gcgcataaaa ccagtcaaag
     6181 gagcgatcgc atgaatttga ctaaacaaga aaaaagaatc tgtcgtcaga taatgttgca
     6241 aattataggt gatcgtgaca tggacgaatt atctcaacaa gagagtcgaa tggttcggaa
     6301 gataagtaag ctaatcggtg cagatacgat ggatcgatcc aagccattac ccaaactgaa
     6361 ttttgaatca tttacgtttg agaaatatag ccatctgaga gaggtaggct atagtgtaaa
     6421 tgctattcga aaagcacttg gggtaggaag tagtgtatgg gcaaaatggc ggaaagaaaa
     6481 tgtaaatgag gtaaatcaaa atgattaagc taataggcaa agagacgctc aaggtttact
     6541 atgtagcgga aacaaaggca gaagtcagcc gttggctatt gaaaatgttt aataagacta
     6601 gagccaatgc ttcagtaagt cagaaagcaa gtgtatcaga aacaggcatg cctgaagcaa
     6661 tgtggatttc aaggaggaac tgagatggca atgataccga agtttagagc gtgggataga
     6721 gaaaaagaca gaatgattta tcctagcacg acaggagttt gttttgaaat gaatgatgat
     6781 ggtataagtg tgcttgatat tgatgtagat tatcccaatg actatggttt tcccaaaata
     6841 gcttcaatcc tcatgcaatc taccactctg aaggataaga atggtgtgga gatatttgag
     6901 ggggatatag cgcttatcac tcataaatca acgaactacg ccgatactta ttggcacagt
     6961 tacgtagaag tttttagagc aaaaaacgga gcctacagaa ttcgcggtga acacatctac
     7021 gaaacagaat tatatagtaa tagaaaacaa ttgacggtag cggggaatgt ttataattca
     7081 ccagaacttt tgaagagaga tcgatttatg actactaccg aaaatccaga gttattgaag
     7141 gaggaacagc gatgaataaa caggaattga ttgataaata tactgcagaa atatctagat
     7201 taagacctta ttgccctaat agacatttaa ttagcgatca acttaaatat ggtctttata
     7261 aagaaatcct agaagattta aaacaactag acgaaccgca gaaaccagcg gtgcctaagt
     7321 ttgtggcgga atggatagaa tacgctaaaa agaaaggcga tagtctagct atttcattca
     7381 agccgtggaa cctctacggt gttgagtata gcaagactga tagatggatt gaagataatc
     7441 aaggaacgtt tgctcgtgct tggctaattg gctacgaggt cgagaaagag ccgttgtatt
     7501 atgtgaagtt gccagttgtg tattttaatc attgggattt agagacgtat ctaatgaaag
     7561 atgatagagg aaatataaca attgcagaca acaacgattt tgatgatatg aaatttacgg
     7621 aatcagaaat aaaagcaatt gatgaaagat actggccatt tgctgtgcca gtggaagagg
     7681 tggcggaagg atgagcaatc ttaaagttgg agatattgtt ttttacaaag gtgacgaagg
     7741 catcatccga gaagttatga cggatggaaa agaaaatttt tatagggtag atgtcagcag
     7801 aaaatctata ctctacctgt atgaagacga attagattta ggtactcaaa aactcaacga
     7861 caaccagaaa atggtgtttg attatcttaa atcaaacgtt gaacaaggtg gcaaatcagt
     7921 tatgtattcg atattccttc taggtgattg ggactctcga attggagcag caagaaacgt
     7981 tgatatagct tactgtggat taaatggtaa gcaggaagca gaagtcctat cagcattcgc
     8041 tcaatgggga ttagaacagg aggaagcgga atgaaatacg aaataccact aagtgaagca
     8101 gacgtccagt caattattaa cggtcgggag gttaataaaa aacttcctga tggtactgaa
     8161 ttagtcatca gacaaagtta tttgaaagat atggcagctc cagtattaat tgatcgtttt
     8221 aacgtgactg attctgtggt agagaaccat ttaaaagaat ttcgatcaag tatagacgac
     8281 actttcagat tagggagttg attgacaatg aacaacagac atcgcagaat aacaaaacta
     8341 agaaaacagg aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca
     8401 gaagaaacat ataaagtggc aagtcagtgt gttgctgatg cgagtaatgc tattcgtaag
     8461 tttgggattt cgatattaaa tgatgatcgt aaatgggagg aaatgagatg aaactaaaag
     8521 acggatttta cgctagtagt cacggcatcg gcggtttaat gctagatatg ccgacaaaga
     8581 atcctaaaac acgtaagaaa ccaaaagtca aagtcggtga catggttcgc tgcgaagcag
     8641 aagggttcat ctatccattt cgtggatatg tagaacacgt ctataatcac tcagcaatca
     8701 ttcgtattga aaacacgatg gaatgtgata aatggacagc taaaagcaaa gagaatttag
     8761 cagtggtgag attggtggat attgaactaa tcaatgacaa ataaaaaagc cggatcgctc
     8821 cgactgattc aataaattcc acaagtttat tatatcacat aaaaggggcg gtttgacttg
     8881 atgcaattgt tacgagaggt agctttcaaa cagacaagat gtaatgcgag agatgtgctg
     8941 aagaactttc ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg
     9001 attattacgg acatgccgaa ggcaccgaag cacggcaata aggcagaaga cgcgatcatt
     9061 cagatgatgg atatagaagc agagagagac gcgattttag cggctttgat ggcgcttagt
     9121 ctgattagtc gtcagatact ctactacagc ttttgtgtgc cagatagctt ctcaaactac
     9181 aggattagcc gtgaagtggg ttattcagaa agaagcatac aacggatgaa gtcggaagct
     9241 ctgatagagt ttgcagaagc atataaacat gggaaaataa ttgcttataa ataggaggac
     9301 tatataatgt ggaaagatta tgtatcattg aaagagttga aaaaagatct tattttcaaa
     9361 aaaatcgtag aatggtcaga gagtgaatta attttagaag atggaaccaa aatggaagtt
     9421 gtatgtagcg aatcagattg ctgcgcatgg gccgaaggtg aattcaaaaa tgtaaagctt
     9481 gatgcagtga ttacagatat aaagattttt gataaaggta accgtctcta taatggcgat
     9541 ggacatagtt cttatgccga agtcgttgtt tatcataatc gaaatgagat tgctaaagcg
     9601 gaatgtacag caaacgatgg gaatggaggc tactattaca gtgtctgtgc tttaaaagtc
     9661 aaagaaaaac tttgtgtagt gactgatgca taaaaaatgg cggttttttg gcggaatgat
     9721 ggcggttttt agctatttac cagtgatatt atggtagtgt cgaaagatta gtgataggtc
     9781 tgagacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa gcttcgatag
     9841 acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
     9901 ttatctatca tccgttgctg tctattgtta ttatgtcact gtggcggaaa gggtagacgc
     9961 ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt gaccatgcaa
    10021 ggttcgattc cttgccagcg acttagacga aaggtagctt aaatattaac cacatcaaca
    10081 tcgtcagcga gttgataaag gtaactagca gactctagga tttaagccag tcttaccgat
    10141 tagtctgtta tagtagcaag cttgctagag gtagctcctc tggttgacgt gcagctcagt
    10201 tggtagagcg cctgactttt aatcaggagg tcgccggttc gaatccggtc acgttaataa
    10261 agaacctacg gaaacaatca atcttatcgg atgccgattg gttggctgaa ttaagcagtg
    10321 tgtctgtgaa tcagttaata ggcataaaat actagcacag acgtgtgcca ctctcaggtg
    10381 taggttagga gagaaacatt agcaaccgag ggttggaaat gggcgctcaa agtacacgag
    10441 caaggcgagg tcgatagtaa tcgatggaat cggtgtaggt tgctattaca taattgattg
    10501 ggttagattg agttttggga ttcggtacaa atgaatcgtc aaatgactca agcacaggat
    10561 cggaaacgtc cctgcctgtg cattacatat tagatcactc gttgagtggt ctttttattt
    10621 tagaaaagag aggattttga aaatgaatca tgagaagttt atcgaaaaat gcaaggctat
    10681 tgtacgtgaa agaattgaga atgagattgc tgacctaagt ggagcagtac ctgagtttag
    10741 tgttttcata gtctggtcat gcaaaacact acagaatagc aaagcattag tcagcgctag
    10801 cttaaaagga gcaccgtatt ttgaaattac gttgaacggg gacaaaggtg aaatctatgt
    10861 agatacttat ctcaaaaaat caaatgaatg tatcaaagtc tagcaggtgc taggcttttt
    10921 ctttacataa aggaggaatg atctcatgaa acaatatacc gctaaagatt tcgaggaaat
    10981 gaagcgatta aagaaggact atgaagaagt tgatatggag ctaactgttg gagtcattca
    11041 acgaagactg cgggtcggat tagagacagc aaaggctatt tacaatgatc taaatgctat
    11101 tgaagagaag aatggctaat gaggaactac tggtatatat cgctaactaa tgaatatcct
    11161 cgaaccattg atgattgttc agtgcgtgtt gtgcgttctg tacaaatcaa agggaagtac
    11221 tctattgtcg aaatggggag agaagcagaa ccatgtgaga ttgatgcgtg caagctcgtt
    11281 tattgcggtc atggtttcta tgatgaacca aacattcaaa ataatattaa caagaatttg
    11341 agggattaga atgcaagaaa tggcttaaaa atctccaatc aggacaaatc aagttagaca
    11401 aagtttccga tttaaagata ttaattgaag cagatctaat gttgaaagat attgaaaatt
    11461 agaaaacaaa actcaaccta agaagattgc gaggtggtgt gtattgaatg gcaagacaac
    11521 gtgatccaag acgtgatgaa gccaaaagaa tttggttaga atccaacgga gaaaagcagt
    11581 taaaggaaat tgcatctgaa ttaaatgttt cagattctca ggttagaaaa tggaaatcgc
    11641 aagacaaatg gagcgctgaa ttgaaaagta acgttaccaa tggcaaaagt aacgttacta
    11701 atcaaggtgg cgctcctatt ggtaatcaaa atgctaaagg taacaaagga aatagccgag
    11761 cttctccgcc agtgggtaat aaaaacgctt tgaaaacagg cgagtatgaa accatatttt
    11821 ttgagacact aagtgatgaa gagaaggaca tctattctag tctgaatgat aatccttctt
    11881 ttgttttgtc tgaagaaata cgtctactta agataagaca atttcgtatg atgaagagaa
    11941 tccaacaagc tgaagctgga ctaaatgatg aagaagtcga acgattgcag cagctaagaa
    12001 agattaaaaa tccgattgaa aaaaatggta aaaagctaga aatcaagcgt gaggttatgc
    12061 aagatgtgca gattagcaga aaaaaacatc gcaaaattga tgatattctt tcaattgaag
    12121 attcattgac tcggattagc aaccagttag ctaaagccat caagcaaatg aatgaacttt
    12181 atatgaatga atacagaact gatttaatta aagctcagac tgataagatc caagctgaga
    12241 caaatgaaat tggcggaaat aattcaggtg aagaaataga agaatggaaa caggcagttt
    12301 taaatgccgc aaacaaacgg gcggtgaaag aaaatgaata atgaatttat tccttttgct
    12361 gatattggtt ctgccattga ttattactat gataaaccag tagctttttg ccaagatatt
    12421 ttgcatttga atcctgatga atggcaagaa aatgttttaa atgatttagc tgaattttca
    12481 aaggtttctg ttcgttctgg tcaaggagtt ggaaaaacag cattagaagc aggagcaata
    12541 ctttggttct taacgtgtcg accctacgct aaagtaatag caacagctcc gacaatgaag
    12601 caactttacg atgtactttg ggcagaggta gctaaatggt taaatgatag cttgatcaaa
    12661 aacttactga agtggacaaa gaccaaaatt tatatggttg gtgattcaga gcgttggttt
    12721 gctacggcta gaacagcgac taaaccagaa aatatgcaag gttttcacga ggaccatatg
    12781 ttgattgtgg tagatgaagc ttctggtgtg tctgatccaa ttatggaagc tattcttggt
    12841 acgctatcag gttttgataa taagctgttg atgtgtggaa accccaataa tattgaaggt
    12901 gttttttacg attcccacaa ttcagaccgt gataaataca gagttcataa agtatcaagc
    12961 tatgatagta aacgtacaaa caaagacaat atagaaatga ttcttaaaaa atatggaaaa
    13021 gaaagtgatg ttgctcgtgt ccgtattttt ggagaatttc ccaaaggtgc gttggattca
    13081 tttatcagtc ttgaaacggt tgaattggct acagaaaaac aaattagtga ttctttagtc
    13141 aataaaacaa cggttgctca tattggtgtt gacgtagctc gatatggtga tgattctacg
    13201 attctctttc ctagaattgc taccagggca ttggagtatg agaagtattc aaaacgtagc
    13261 accatggaaa caacaggata tgtcatcaac atggccaaga atctaatgag tcaatatccg
    13321 agtattgata aagtgatgat taaagtcgat gacactggtg tcggaggtgg tgtaaccgac
    13381 cgcctagaag aacttataga agacaaacat tatccttttg aggtgtttgg agtgaataac
    13441 ggttcaacat cagaagacga tttttacgat aatttaggta ctcaactatg gggaaacatc
    13501 aaggaaatgt tagaagaaaa tatgacagca aatcttaacg gagaacagcc tgttattgaa
    13561 ttgccttctg atagttcgtt aatcaaagaa ttaagtactc gcaaattcaa aatgacaagt
    13621 agaagtcgta tacgtttaga aagtaaagat gatatgaaaa agcgaaatat tggtagtccc
    13681 gatattgctg acgcactggc tttagcgttt tatgagccac caagtcacta tcaatttatt
    13741 caattttagg aggtgagctt ttgattagta cagtattatc tttagagaga tataagaagc
    13801 tacgtgttaa atatgcaaca caaattgaag atggaatgtt tgatccgaat ggttttatag
    13861 aagatatgaa accatttttt gctgatcgcg aaagaaaata tctagcttat actagcgaaa
    13921 agaatgaaat agataataga ccaaagccta acactgatat tgtaaaagtt aataataaac
    13981 ttcatgctgg tatgtattca attgtcgtag accaagcagt caatcatttt actggtatac
    14041 ctatcaaatg ggattacgat gtatcggaac aaaaaagaac actcattcaa agattaaaag
    14101 ataaattctt aaaaaatgat atagaacttc cgacagttcc agaagctttt aacaaattaa
    14161 caagcaatct tgattccatg agattcgcaa tgcttgattc tgaaactgca acttttcaag
    14221 gagcttgtgg agtagctttc cgattgttag agcctgtgga agaagatgat ggttggaagt
    14281 taagagcaag taatattgaa ccttggagag cagaaagata tggaaatgcc ggaatctata
    14341 tcaaagaaaa gtatgactct taccagaaaa aattttttca agaaatgaaa gttatgacga
    14401 gaaataaaat tctgacatat gcttgctatg gtgatttgaa ttttgttaca agcggaacgt
    14461 tcaaaaaaat tgatgagacc gataaccctt tggggacgat taccttggca gaatttaaaa
    14521 ataatacgaa tcgttattgt gattttgaag tagctgagga aattggagat gcaattgatc
    14581 gtgctttgtc tgatcaacaa aacgaaatcg aacaatttaa gcttgcgtac atgcttgtta
    14641 ctggcacaac aatgagcaaa ggcactgcta aagaaatgat gaatcagcta gggattatca
    14701 acttaaaaga ccctactgca aaagctgaat atgtcacaaa aaatttagca aaagatttca
    14761 atgaatatca tatggatcta ttgaaaaaac agttttacac catttgtaaa gcaatcgatt
    14821 ttaacgatga ggtgtttaaa tcaaacagtt ctggagaagc tcgcaagtgg caaatcatta
    14881 gcctagaagc gaaaacaaat actaaagagc aatattttag agaaggattg aaagaatgcg
    14941 cagaaacaat tgctgctttt cttaagtttc atgacaaagt agaaattgag cctgaaaaaa
    15001 ttatttttac tttctctaga tctttgccaa ctgatttaag ttatttagct gaagctttac
    15061 ctaaacttgc accatacgta tccaaacgaa ctattcagag ccaaattcca tttgtaacag
    15121 atgttgatta tgaaaatgaa atgatggaat tggaaagtgg aagtgcttat ccagatagtg
    15181 aatacaattt tggcggaggt ggcaatagtg acgatagaaa cgaagtattg gaccaaacgt
    15241 cgagaactgg aggatcaagc aaggctcaaa caagaaaatc agacacttaa aaaattaact
    15301 agtgtatttc ctgaagcact gaaagagata caagcaaaac tattatcaca agctgactta
    15361 cacaatatca cttatccaga aatgatggag ttttatagta caagtaatca gaaaaaatat
    15421 cgtgaatatg tggaaaaaaa ttataagtca ttaaaaatgt atgatgcaaa atacaaagag
    15481 tttatcgatg aattttttcc accatttgac tatgcaaaag tcaatcgctt attacaaata
    15541 cgatcagatg tatttaaaat tcttgcagaa tatgcgatgg atgcagatgt gaatcaatat
    15601 ttttctgatc gcttagagga aattcttcaa agaacatatt cttctaatgc taatgttttt
    15661 gttcaacttt taaacgttga tataccaaat tatttaccag aaaatgaaat taaacattat
    15721 ttgaactatc catggtcagg aaagacattt tcaagaagac tttggggaaa tatttcatcg
    15781 cttgaacaga aactctctaa tgctattgta aaaagtgttg ctagtggaga aggcgttata
    15841 cacgcattaa acaccatgag attagactca gaaatttgtg acatgtttaa gttagaagaa
    15901 tcaaagtata acaaagcgat agaaaatctc gttcgaacgg agtatgcaaa atttgcacaa
    15961 gatggtattg aaaaatcata tttagaaaca ggtattgagg aatacaacgt attgactgca
    16021 aaagatgaga gagtttgccg gatttgtgga ggaaaggcaa gtaagaatcc ctataaactg
    16081 aaagatgctg tcataggtga aaatcgagca cctttccata gtcgttgtag atgtacggat
    16141 gttcctaatt taccaaaatt aggaaaggat attgatgaag aatatgaccg tttatttggc
    16201 gatttattag atgagtttgc acatgattct tttggaatta atttgaaacg gaggaagtag
    16261 aatgaaagat ttttttgaag cagtactaac aattaatgta aatgctgata ttgcagaagc
    16321 ctacaaaaca gctattgagt ctgagaacca tcctaatggc ttgagagacc attggaatgg
    16381 caattatgcc tacgtggtta ttggcgatca aactgttaat tatcaagata atactccagt
    16441 tgataagaat accgttaatt taacgattca attattatct cattcattac caaatttaaa
    16501 agaaacagtt gattggtatg aaaagatggg atgtattgtt gttagaactg actacaaaga
    16561 aggaaagtct agtaattagg cttttttatt ttgtccgaaa tgacgttaaa ctagcgcaat
    16621 actgggcttg gttgaatggt ggggcgcaac tattaaaact caaagcaatg cggggcgtga
    16681 aaacgaatcg tggggcgaaa ggagaatgag catgaaacac aaatcattaa tgccaatgaa
    16741 tttgcaatac tttgccgaag gtgacgatca aaagttttct tttgatgact tcaaatcttt
    16801 tgtggaatcc aatgaagaag ctcaaaaatt tattcagtca caatctcaaa gtgttgccga
    16861 caaacaattg gaagcttgga aacaaaataa cttagataaa attaaacagg ataccatcaa
    16921 ggaatatgag gaatctaaga aaaacaagtc acctgagcaa attcaactgg aaaaactaca
    16981 agctgaattt gaagcagaaa aagcgttgcg tgtgacaagt gataataaag catttgttgc
    17041 agaacaaatt gctggattag aactagatgg agagttaaaa gagtctattt ctcaatttat
    17101 gctaaataat cttgttagtt cggatacaga tttcactaag aatgctgttg aaggttttac
    17161 aagtgtcttg aatgcaatta atgagaaaca tgcagatgca ttaaaagaac tacaaatgaa
    17221 gtctgcattt ggtggaactc aacaatcgaa taaccaggtt cagcagaaca atgaaacatt
    17281 aacaaatcca gaagaacaat taggacaaat tcttcaacaa tttaactagg agagtgaaaa
    17341 attatgaaaa aaacatcttt aaataattta gagtatttgg atatttcacc agcgattaat
    17401 gctatgcaag taccaaatac acctttttta agctatttat ttggtgctgg aaaaacagag
    17461 ccagcaaact cgacagaaat taaatggcgt gaatatgata tcaacaacga tgattcttct
    17521 gaaaaacttg agggcggaga atatccagat gctgaatcag gtcgaacttg gtttaacaac
    17581 tatactgaaa tttttagaaa atcaacctct gtatctggta cattagatgc tattaatgtg
    17641 aatggagtcg gaaatgaatt aactaatcaa gtagcactac gtggtatgga aatgaaaatt
    17701 gatttgaacc gaaaattgat tactggtgta aaagctgatg aaaatggttc taaaggtcgt
    17761 cgaatgaatg ggattttgaa cttgatcaat tcagcaaata aggcagaaac agccactgcg
    17821 ggtgcagtaa caagaaaaga tatcgatact ttatttaaat tgatgtatga aaaaggttat
    17881 atgggagaaa aactatgctt gatttctcca gatatgcagg agttaatgac tgatgagtta
    17941 gatggaaaat caacaaaaat tgttcagttc ggggaaagag taacttttgg attgcaaatt
    18001 ggaaatatcg tgtctaatta cggtacaggt attgctctac tagaaccatc attgccaaaa
    18061 ggaacaattg ccgcaatcga tactaattat gtgaaactac gtccattacg tgaatggaga
    18121 gcagaagaac ttgcaaaaac aacggattct agacgtatcg gtcttgtagg tgaatactct
    18181 cttgaataca atgcttcaaa ctctggggca attttaaatt taaagtctga ataaaaggga
    18241 gttagtactc ccttttttga taggaggaat tacagtggta aaaaaagatg aaactaaaaa
    18301 agatgaagtc gtgaaatata gagtaggtaa aactaaaaat tttgttgggt ttgttcatcc
    18361 taaaactcgt agatttatca cagcagattc aaataacgaa tttatcattt ctatagatga
    18421 taaagaagca attgcaattt tggaagatgc aattgatgtt aatgaaattt aggaagtgat
    18481 ctgatggatg aatcgctaaa aacggaaatc attgagtcta caaaagaaga ttttccagat
    18541 ttgagtgaag aacgcataac taatttatta gaaataattt tgctagaaat tgaatcatac
    18601 aacacttgta aaaatgatat ttcatgggaa aagttaaaaa gcgtgattag tgaagtgttg
    18661 tataaaataa taaaaaatga atcagaaaaa acagtatctt cagttagacg tggcgataca
    18721 acgattagtt atgcttcaac aacaaatgat gttagtgagt tacttctagg ctatggtgat
    18781 ttgatacgaa gagttattgg ttgtggagga ttggagtttt tttaatgaat gaagcagata
    18841 ttttagaaac tacttatgaa gatagttgta ttattgaaag actgacggac attgaagatt
    18901 ctaatacaaa tattactatt caagattaca aaaaagtata tgataatcct atttcttgcg
    18961 ctctttcaca aggtcaaatc gatggactag cagtcataga agatggagag atggtaaatg
    19021 tttcaactga cacatataaa ttatttgttc atcctaagat taaactaaaa aaaggagatc
    19081 gaataacaat aactcaaaag gccagtggct taattttttc tctatttgct actaagcctt
    19141 tttactatcc tagtcattgt gaagtgaatt tgataggaag tgagaaaaat gggtaatctc
    19201 aaatatgaat caaatgcaga aaagatcatt gagaatttta aaaatatgac tgtaattgct
    19261 caaaaagaag gaatatcctt cgttaacgat tcaatgaata aagtcgttag tttagctaaa
    19321 ccattaactc ctgtaaaatc aggtaattta aggcgaggat atcgtgtagt aaaagctaga
    19381 aaactatcaa gcggtcgaat tgttggagca gttataaaca atgaacatta ttttaaatat
    19441 gtagaagaag gtcataggac taaaaatggt ggctttgtca aagggaaatt catgttaact
    19501 cgtgcaacga atcttgcaaa tatgtcttat attcctcgaa gatttaaaca aatggcaata
    19561 aaaatcgtta agaaaggaaa gtaacatgaa agatgaaatc attgctgcaa tcagtagcaa
    19621 attaaaagaa atctatcctg atgggacaat atatcttgat tcagttatgc agtcaactaa
    19681 agatttttat ttcgttttat cagtaatgga atctggaact gaaaatgtag gaattgatat
    19741 tcaaaatgtt tctttcttaa ttgatattgc attgattgat aataaaccta atagaaattt
    19801 aattaatgaa ttagtctcac gttgtgggac tttttttaat acgattacaa tcgatgagca
    19861 aatattattt ccaaaagcct atttacctga tgaagcagat ggtgttcaac atattcgttt
    19921 tacattagaa tttccacaat atattgaatg gagtgaaaga taaatgggag aaaaaagaag
    19981 taaaaccgga attatttctg tcgaaaaacc tacttggttt cctttggaag atgagacagg
    20041 gaattttccg gtatatggta ctgcgttgcc aatgggaaca gcagtaagta tcaaaccaac
    20101 agctaattat gaaacaacac aagattatgg ggattcagtg gttcaagatc aatttacagc
    20161 ttttggtggt gcagaagttg aactggaagc aaatggatat actcataaag ttttatctac
    20221 aattactgga gggaaaattg ttaaaggtgg tgccttgcgt tctggagaag atattgcgca
    20281 agatggtgcg tttgcatata gacgtaaaaa atcgaatggg aaataccgtt atacggtctt
    20341 ttataaagga cagtttgctc tagattcaga tgaaacatca acgattgaag gaagtaaagt
    20401 aagttttact catccaactt ggaagggttc atttgtagat gtaccaggac ttggatatat
    20461 gtattcagtt gatgaagatg atgaaggcgt ggataaagca atgatcggaa attggtttac
    20521 aaaagtagca attccaattg aagaagcaga actttcagga ggtacagaat aatgtcaaaa
    20581 tatcaaacaa cgattaaatt aacgaaaaaa gatgaagaag gtaaatatga gcaagtacaa
    20641 tttaaatctg ctgaattttt accaggaaca gttgtagaag atgctgcagg agttatggaa
    20701 gaaatgcaaa cagcgactga taaacaatca gttaaaaaag ctttaagtcg tgcttattca
    20761 tttattgcag atactttatt tgagggacaa tttactggtg aagattattg caaaggaatt
    20821 gatgctcgtg agattgcctc attgacagga aaattattga agtctgttac tgcaggtttt
    20881 gatgaaactt atacagagac gaaaaaaaag taagtgaggc gctcaagtca ccttcattta
    20941 agtattcgat tacttaccga gaattagata taaagacgca attactcgaa gcaggttgga
    21001 cgttaccaga aattgaacgt actgacttgg atgagttaat gcgtcttttt gcttttagag
    21061 atgcggtaaa agagcatgaa gatgttgagt actatgataa tttcactcaa ttttaggagg
    21121 tgatactttg aataacgatg acctaattct gaagatgata ctagatgaat ctggttttac
    21181 tgccggtatg aataatgctg tcaaaaagtt gaactctttt gatgaaacaa tcgaaagaaa
    21241 aagtagaaat agtggcagtt cattaggtag tatttggaaa atatttgctg gaagttttct
    21301 agctagcgga gtaactagaa tcgtaggagc tggctttgat ctaatcaagg gttccataag
    21361 tggagcggtt gatcgagtag atacgatgaa taacgctcta cgaaatttcc aaaatatggg
    21421 atttagtaat tcagaaatta tgaagaatat cggaaagaat gggcttttat ctcaaggtat
    21481 tcaaggactt cctactgctt tgaatgatgc gataagtcat gttcaacttc ttgcttcttc
    21541 tacaggtgat ttaactcgtt caactcaaat atttaaagct ttaaatgatg gaattcttgg
    21601 tttcggtggt tcaactgatc aagttaatga agctgttatt caattatctc aaagtttctc
    21661 aaacggaaaa gtagacgcac aaacttggaa ctcaatgatc aacgctcaac ttggacctac
    21721 tctttctgct attgctaaaa agatgggaat tacaatggga gatctgaaag aaggtttgtc
    21781 tcaaggtaag atttctgttg aagaattcca aaatcaatta atagaaatgg ataccaaagg
    21841 tggcggagga cttaaatcat taagtcaaat cgctaaagat tctactaagg gaattaaaac
    21901 ctctatacaa aatgcaaaaa cagctgttac acgtggtgtt ggtgaggtaa tagaaggatt
    21961 aaataaagca ttagtagatt cagatttagg tggatttaag ggaattattg ataaagtggg
    22021 cagttcaatg gaatcgttct taaaagtaat tgctgcaaat attcctacag cagtatcatt
    22081 tttaagtaat ttatttaacg aaattcaaaa atttggttct gctttgaaat tcatgatgcc
    22141 ctttcttgtt cctgcagcta ccgcttttgg agcgttcatg tttcaactta aaggtatacc
    22201 agcaataata aaaagcttca ataattttaa gaatgccata attggcgttg gaaattcatt
    22261 aaagattatg ggggcaatag ctgctgctaa ttcatttgtt ttaattattg gtgcagtcgt
    22321 tggtgcaata gctgttttcg gttactttat ggcaaccaat gaagagttta gaaacaaggt
    22381 tatatctatt tggaatgatg tgaaagattc cgtaattggt gtattaaaaa atataaagga
    22441 ctggggaatt gatacttgga attctgctaa agaaatggca tcaaatgcag ttgagggtgt
    22501 caaagatgct tggtcaggaa caaaggaatg gttttctaac acctggaaag gtattaagga
    22561 tggggccaca ggtttatttg ataaaacagt agagacttca agaaatgcag tcgatagtgt
    22621 aaaaaatgca tggtctaata tgaagaaatg gttttctgat acttggcaaa gtataaaaga
    22681 ttcagccaga gaaaaatgga atgaaattaa aggttctatt atgtcggttg ctggtccatt
    22741 gattacaggt ataaagaatg catttttaca tgttactttt tatctagaga ccttatggaa
    22801 taatttagtt gagattggta aaaacgtctt tgagatttta aaaaatgtta tacttgcgcc
    22861 ggttttgttt attacctcca tgatttctgg tggatgggaa gaaacaaaaa ataatatgat
    22921 tgctgtttgg aataatatta aaga
//
LOCUS       DAJSVD010000039        22148 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSVD010000039 DAJSVD010000000
VERSION     DAJSVD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.039"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            218..2053
                     /gene="tdc"
                     /locus_tag="ON498_001344"
     CDS             218..2053
                     /gene="tdc"
                     /locus_tag="ON498_001344"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3939605.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2358..3836
                     /locus_tag="ON498_001345"
     CDS             2358..3836
                     /locus_tag="ON498_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3939606.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3802..5133
                     /locus_tag="ON498_001346"
     CDS             3802..5133
                     /locus_tag="ON498_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3939607.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5247..7799
                     /locus_tag="ON498_001347"
     CDS             5247..7799
                     /locus_tag="ON498_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR3939608.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7806..9245)
                     /locus_tag="ON498_001348"
     CDS             complement(7806..9245)
                     /locus_tag="ON498_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939609.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9591..9953
                     /locus_tag="ON498_001349"
     CDS             9591..9953
                     /locus_tag="ON498_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3939610.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10140..10997)
                     /locus_tag="ON498_001350"
     CDS             complement(10140..10997)
                     /locus_tag="ON498_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3939611.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11117..12028
                     /locus_tag="ON498_001351"
     CDS             11117..12028
                     /locus_tag="ON498_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3939612.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12035..13681
                     /gene="mdcA"
                     /locus_tag="ON498_001352"
     CDS             12035..13681
                     /gene="mdcA"
                     /locus_tag="ON498_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR3939613.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13783..14082
                     /locus_tag="ON498_001353"
     CDS             13783..14082
                     /locus_tag="ON498_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR3939614.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14072..15748
                     /gene="mdcD"
                     /locus_tag="ON498_001354"
     CDS             14072..15748
                     /gene="mdcD"
                     /locus_tag="ON498_001354"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR3939615.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15752..16345
                     /locus_tag="ON498_001355"
     CDS             15752..16345
                     /locus_tag="ON498_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR3939616.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16369..17445
                     /locus_tag="ON498_001356"
     CDS             16369..17445
                     /locus_tag="ON498_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3939617.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17470..18339
                     /gene="mdcB"
                     /locus_tag="ON498_001357"
     CDS             17470..18339
                     /gene="mdcB"
                     /locus_tag="ON498_001357"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR3939618.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18450..19214
                     /locus_tag="ON498_001358"
     CDS             18450..19214
                     /locus_tag="ON498_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR3939619.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19703..21796
                     /locus_tag="ON498_001359"
     CDS             19703..21796
                     /locus_tag="ON498_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3939620.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21821..22123
                     /locus_tag="ON498_001360"
     CDS             21821..22123
                     /locus_tag="ON498_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3939621.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3785 c         4718 g         6468 t
ORIGIN      
        1 tatctatttt agaagactga tttattgaaa aattgttcgc ttttttgcgt taaattatca
       61 gaaaaatccg actttattta tttttgtttg taaaaatcta cgtggatttg atgttataaa
      121 aaaaaaattc tcatttttgg cgctatagtg aagaggtgtt acagataaac acatgtatct
      181 gcaagacatt gaccaacaaa atatggaggg aagaaaaatg aaagatatgg atatcaaggc
      241 cgtctttata ggcgacaaag ctgaaaatgg accagtttat aaaatgttgt tgaacaagat
      301 ggtagatgaa catcttggat ggagagaaaa ttatattcct tcagatatgc ctgcaattag
      361 cgaaggagat aagctcactc cagattatct agccacccgc gaccatatga tagaggtctt
      421 ggatgaagtc agtcagcgat tacgcgcagg atcgatccca tggcattcag caggccgtta
      481 ctggggacaa atgaatgctg aaacattgat gcctgcttta ttagcttaca actatgcgat
      541 gctgtggaac ccaaataatg ttgcattgga atcttctatg gcaacttcgc aaatggaagc
      601 agaagtagga caggattttg cttcattatt caatatgaca gacggatggg gacacattgc
      661 agcagatggt tctattgcta atcttgaagg attatggtac gcccgttgca tcaaatcgat
      721 tcctttagct gtcaaggaag tgctgcctga aaaagtgaaa aaaatgtctg agtgggaact
      781 tttgaattta tctgttgaag aaattttgga aatgaccgaa agctttacgg acgaagaaat
      841 ggatgaggtc aaagcagctt cttcccgtag cggaaaaaat atccaaagat tagggaaatg
      901 gctggtccct caaacaaaac actattcttg gatgaaggcg ttagatattt gcggagtcgg
      961 cttagatcag atggttgcta tcccagttca agaggattat cgaatggata ttaatgcttt
     1021 ggaaaaaaca attcgtgaat tagctggaca aaaaatccca attctaggtg tggttgctgt
     1081 cgtaggaacg acagaagaag gtcaagtaga cagtgtagat aaaatcgtcc aattaagaga
     1141 aaggttaaaa gatgaaggga tctatttcta tttacatgtt gatgccgcat atggcggcta
     1201 tgcgcgttca ctgtttctaa acgaagcagg agaatttgtg ccgtatgctt ccttagctga
     1261 attctttgaa gaacatcatg ttttccacca ctgcgtaacg attgacaaag aggtatatga
     1321 agggttcaga gctatttcag aagcggattc tgtcacgata gatcctcata agatgggcta
     1381 tgtcccttat gctgctggag gaatcgtaat caaacataaa aatatgagaa atatcatttc
     1441 ttatttcgcg ccatatgtgt ttgaaaaatc agtaaaggct ccagatatgt taggagctta
     1501 tattttggaa ggatcaaaag ctggagcaac cgccgccgca gtctggacag cacatcgtgt
     1561 attgccattg aatgtgaccg gatatggtca attgattggg gcttcaatcg aggcagctca
     1621 aagattcaga gaatttctcg atcatctgac ttttactgtt aaaggaaaaa caatcgaagt
     1681 ctatccattg aatcatcctg attttaatat ggtcaattgg gtattcaaag aacaaggctg
     1741 tacagatttg aacgctatca atgaattaaa tgaaaaaatg ttcgatcggt cttcttatat
     1801 ggatggcgat gtttatggtg aacggtttat tacttctcat actacattta cacaagaaga
     1861 ttacggtgat tctccgattc gttttgttga aagaatgggt ctaacgaaag aagaatggaa
     1921 aaaagaacag aaaatcactc tgctacgtgc ggctatcatg actccttatt tgaatgatga
     1981 tcgaattttc aatttttata caaaaaaaat cgccaaagca atggaaaaga aactaaacga
     2041 aatcatccaa tagaactgat ttttgtgtga aagaataaca agagacatat ggagaacaga
     2101 caattatttg cttcatcaaa acaattgtat ttccccctgt ttttcatata ttcctcgcca
     2161 agatattctt ttttataaaa cgttctataa gttggatcgt tcgtttcatg tatcaaaccc
     2221 agttcactaa gatcgttcaa tggatgtttc ggtgatactg ggttttgtac tgaatcgatg
     2281 aaaccttgtc atttctgttc ttgaacaaaa ataaaagaaa acaaaaacat atgtcgctat
     2341 tgaaagaagg aataaagatg aaagatactg gttcatcaaa gcaaatcacg accatgacct
     2401 ttatcggaat gacctgcgcc cttgtagcaa gtgtacgaaa tattccagat gtagcagcaa
     2461 cgagctggac gatgattttt tacatgctgg ttgcagtatt attgtacgct tttccaatct
     2521 cattgatctc tggtgaattt gctggaatgt ttccccaaaa aggtggtcca gaactttggg
     2581 tatcgaatgc tctaggaaaa aaatgggggt tcgttgtctc atggctgtta tgggtccaaa
     2641 tgtttcctgg gatggtgatg gtcgcttcag cactcgctcc gttgtttggt aatattatcg
     2701 ataatgtgcc gcttggtttg aacagcaaat ttacattagt tgtcatttta gtcgtttatt
     2761 ggatcatcac ttttttgaat ttgaaatttg atatggcaaa aatcggcgga aaagtcggtg
     2821 tttggttagg attgtacgtg cctttgacga tgatgctctt attagggttt gctgcgtgga
     2881 tcaaagttgg gatcgtacca accggaacat taggcagttt ttcatgggac aaattaatac
     2941 cagatactac gactgcatcc tcatttgttt attttgcgcc gatcatgttt atttttactg
     3001 gtatcgaaat gtcctctgtg tatatcactc gattggaaaa tccagttaaa acgtatattc
     3061 gcggagtatt tgccgcgtta atttttatat ttttcgtcaa tattttgaat gctttagttg
     3121 tagcaaatgt cgtaccaaaa ggacaaatgg aattgaataa tatcgcgcaa tctatttcga
     3181 tttattgcca aattcttggt ttgccacatc ttattgtgaa tctgtttagt ttacttgtct
     3241 tcattggtgt agcagtccaa ttatctgctt gggcctcagg accagcaaag acagtgacag
     3301 aaagtgctag aaaaggtgcg tatccaccaa aattcaattt ttggaagacg aatcaatttg
     3361 acgtatccaa atccgttatt ttgactcagt ctgtcattat ctcaatcttt gcactttttt
     3421 atttgctgat tcctggcgtc aatcaagcat ttttgatgct cgtaaattca actactgtta
     3481 tctactgtat cgtctatgtc atcatgggga tcgcagtttt gagattgcgt tatacacatg
     3541 caaagttgaa tcgaccattt agaattggaa aaaaagagaa aaagagcaat cttggagttt
     3601 ggactgtcgt tattgtttta tttgcggcaa tcggattttc tgtcggactg acgatgaaag
     3661 ctggaacatg gatcaatcta attgcagtca cagccatcag tgtcattttg tttgtagtac
     3721 cactctgtat tgaaaaaata aagaaacctt cttgggaaca ggaagtcgcc atgctgcttg
     3781 aaaaagaaaa aggtgaactt gatggaaaaa ccggcaatga aacggactct aactaatcga
     3841 aatatccagt taatcgcatt aggtggcgca attggaacag gattattttt aggttcagca
     3901 aaggcaattg catcagcagg gccagctgtt attctcgttt atttgatatg cggtttggct
     3961 atttatggtt tgatgcgggc catgggtgat ttatttttag caaatagtac atttaactct
     4021 atcagtgatt tcgttgacta ttatttaggc aaaaagtggg cattttttgt caattggact
     4081 tattggcttt gctggcttgg agctggactt gcggaattaa cagcaatagg attgtatatc
     4141 catttctggt ttgtacatgt accagctttg ataagcggag gtagcgcact aatgatctta
     4201 atggcagtca accttgttgc agtcaagtgg tttggtgaat tggaatcttt tttctcaatc
     4261 atcaagatct tggggatttt cctttttatc ttattgggta taggattgag tgtctcagat
     4321 tttcataccc agtctatcgg aatcaaagaa aattttggac agatcgatga attactaccg
     4381 tttggctttg ctggaatggt agtctcattt cctatggttt tattcagttt tgctggaatt
     4441 gaaatgatcg gcttaaccgt tggcgagaca agtgatccgc aaaagaattt gaaaaaagca
     4501 atcaactgtc tgccttggcg tattctcttt ttctatatag gaacgatttt gtcgatttta
     4561 tttgttgttc catggcattg tttagattta aaagaaagtc ctttagtgac tatgctgcaa
     4621 gtgatccatt gccaaggcgg agcagcaatt gtcaatctaa tgattttggt tgcttctttg
     4681 tcctcagcaa atagtgcagt ctacagtact agcaggattt tatatcaaac agcaaaagaa
     4741 aaaagtttgc cccattggtt ttgtgcgctt tctaagaaag gcgtaccttt acacggtatt
     4801 ttaattacag gaatgttatt ttttgccggt ctttgtctac atttttttgt agctgatagt
     4861 cgattgtttt ttcagctttt ggcaggcatg acaacgagtt gttttctatt tgtgtggtct
     4921 tcgatcttgt gtgcacatgt tcaatttatt aaagaaaaga aacggaacac tacacaaaga
     4981 tataaagatc ttgctttgtt ggtgtttttt acttcaattg ctttcagctt gttattcgaa
     5041 agcacgacac gctttattcc ttttttattg gttatctggt ttgtattctt aaattttgta
     5101 tataaaagaa taagcaatag aaatatcagt taggcccaga tacattttgc aaaacgaatt
     5161 atttcagacc cccctgaaat aatcgatacg cctgaaaaaa cttccctgcg tataataaca
     5221 gtatcaatta aacaggaggg gataggatga actggtacag attgacggta gaagatgtat
     5281 tgaaaaacta tcaaacatca acagaaggct tgtcgcaaga agaacgacag aaacgcctga
     5341 ctgatcaagg atataatgaa ataaaagcaa ggcagcagac aaaacgatgg aaaaaaatag
     5401 caaagcattt tactgatctg ctgatgatcg tgttgatcat tgcctctttt ttgaaatttg
     5461 cttctagtga atatattgaa ggaagtatta ttttattcgt agtgatcgtc aatggactcg
     5521 ttagttactg gcaagaaaga aaagcggaag aatcattgaa tggtttgaag cagctgatgg
     5581 gacaagaagc gatagtacta tcaaacggga tgcaagaaaa aatccctgcg aaagaactgg
     5641 taaaaggaga tgtcattgtc ttacgtgccg gagatgtcgt tccagcagat atccgattaa
     5701 tcgaagcaca tgatttatta gtggaagaat cgattttgac gggagaatca gaagcatctg
     5761 aaaaaagtca tgccgttttg acagaagaag aatcgatcgg cgaccagaaa aatatgggtt
     5821 tttcaggaac gcttgtacaa tctggttctg cagtcggtgt cgttgtagaa acaggaaaca
     5881 cgacagaaat cggtaaaatc aatcaagcat tgcaatcggt cgaacaacaa acgacgcctc
     5941 tgattaaaaa aatcaaccga ttaaacaaac aaatttttca aggcattctt tgtttgagcc
     6001 tattcttagt tatttttacg acatttcgat atggtatgga ttggcatatc cttctttcgg
     6061 ctacgattgc tttggtggtg tcaatggtac ccgaaggatt gccagctgtt ttgaccatga
     6121 ttctatccgt tggagtacat gaaatggcaa aagaaaaggc gatcatcaaa ggacttcctt
     6181 cggtagaaac attaggttct atgacagtga tctgttcaga taagacagga acattgacaa
     6241 aaaatgaaat gacagttgtg gatgtagctg caaaagaaga agcctgcgtg ttgtccatta
     6301 tgaaaaactg tcaggaatta aaaacaaaag aagaacagaa aacagaaaat ttaagtggca
     6361 atccgacaga agttgcgctg attcgacaca cagaaacagc caatctacct ttgaaaaaaa
     6421 tagaagcaaa gattccgttc agttctgaat acaaatatat ggcaacgatg catgcggagg
     6481 aaaaaggagc ggtgatttac gtcaaaggtg caccagaagt attatttgct aaatcaactc
     6541 tttcttctgc agaacaagaa gcatggagtc agacagctgc agaatttgcg agaaaaggtc
     6601 agcgtgtttt aggatttgct tataaaaaag tagattcgaa acaggaactc acgcatgaga
     6661 ctttaaccca attaactttt gcagggatcg ccggattgat cgatccgcca aaagaaagtg
     6721 cagtcaaagc tgtaaaggaa tgccaacaag cagggatctc agtgaaaatg attacagggg
     6781 atcacaaaga cacagcaaaa gcgattgcgg aacaaattgg cttaaaacat actgccaaag
     6841 ttttagaagg gatcgatttg gatcttatgt ctgatgaaga gttgattcag caagtgccaa
     6901 tagtcgatgt ctttgctcga acgacaccag aacataaact gcgaattgtt aaagctttgc
     6961 agaaaaatgg agaaatcgtc ggaatgaccg gagacggcgt aaatgatgca cctgctttga
     7021 aaagatccga tgtaggaatt gctatgggaa tcaaaggtag tgaagtaagc aaacaggcag
     7081 ctgatatggt tttaggagat gataatttcc atacgattgc aaaagccgta aaagagggcc
     7141 ggcggatttt ggataatttg caaaaaacca tcaatttctt cttgccaact gcattagctc
     7201 aaggattgat cttgatttgg gcacttatgc tgaatcgccc gctacctttg tccccagtac
     7261 agattttatg ggtcaatatg gtaacgacaa tcactttatc ttatgcacta gggttcgagt
     7321 cgccagatcc agaaatcatg aagcgcccac caagagatcc aaagcaagga atcctatctg
     7381 gttatcattt gtttcggatc atctatgtct ccctactgat catggttcct gcctatttat
     7441 tggctatgca gtttgaaggg caaagcttac agcaaaccat tcttctgcaa aatatcgtct
     7501 tagctcaagc tgtttacatg atcaattgca gagagttgtc caagccgtca ttgaaccaag
     7561 gtcttttgca aaacaaagcg ctttttcttt cattaggtat ccttgcttta ttgcaaggga
     7621 tggttatttt tctaccagtc ggccagcagc tgatcggtac agtaagttta acgatgacac
     7681 aacagttatt gattggagca aatgtgattc ttctattctt agttgtagaa atagaaaaag
     7741 ggatcatgaa caaactgttt cgcaaaaaag aaaaaacagc tagagaatac agtcgctagc
     7801 cgttactata aaaaagtttt gaaagtgagt tcagtcaaac gattgagctc atttttcgtt
     7861 ggaatagaag aaatgtatac aatagggata tctagagaat ctagagggaa attggaaatc
     7921 aagatatctg ttttttcaaa agaaatagtg gaaagatcgg ttagttcgat aggtaagaag
     7981 cagacacgtt ttcctaagta atcttttaat tcctgctgca agaacgcctt ccatgaaggt
     8041 tctccttgga aaataaaata gacattgaac aaattcggtt tgatagaaaa aatcgtttgt
     8101 tggatcagta gagacaagag ttggagaaag taatttggtt cattgatttc tatttctttt
     8161 tttagatcgt tcagtaaatt ctctttctgt tgatacaagt gtgcatattg ctgatcatat
     8221 gtttctaaaa aacgatggta ttgacggtcc aatgcttcaa tcaatatcgg cgattcatga
     8281 tattttaata tgaggagagc caagttatca gtcagagatg ttttttttag ttgatcaaga
     8341 gacagctctg aagctagttc ctctacaaac gtttcgagga cctctaaaaa tggagaatga
     8401 tgcaggcagc gagcggtttc ttgtccatag ttattgttgt aattccaact ttccagaaag
     8461 cagaaagaag caaaagaaag ctcagtgtct ttaagataaa taccttccgt taaatacaat
     8521 tttttcaatg cagagtcata cagggaggag agtgtgctgt tggtatggat agaagaatct
     8581 aatcggcaat tcgcttttat acggatcgta ttgataaaga accaacatgt tccgaaaatc
     8641 tcttctgttt caaccgctag ttcagtttga ctgaggtttc ttaaaaattg aaaataatgg
     8701 gattcatgga tcgaataatc ctcgaaaaaa tagttgttat gagtgaaagg aatcagcaat
     8761 cgatgataaa aagtacgaat cgcacgctct tttccaagaa tacaagctgt atatttcgtc
     8821 aaatcgatct gcaagtcgta acttgctaaa cgctgattga ttttagtcac atgtcttttc
     8881 aatgtcccat aagaaagccc atggtcggct agaaaatcag ggacagcaac tttttttgta
     8941 aaaagcagat ttttcagcag ttcgacactg actgattgtt tgaaaaaaat ctcccaaagg
     9001 tcatttgtta ataaattttg ctggttttgc aaactgattc ctgcttgatc gctgatgatg
     9061 tcccagcctt cgggcagctg agagcgaata gattgaaggt cagaaaaaat cgttcgttcg
     9121 gttgtcccta tgatttcagc taattcattt acggttatct ttgtatgatt cagcaattgt
     9181 tctactaaag aaacatgacg tcgaatatct ttttcggtaa tgattttttt cattagtgaa
     9241 tacatcaatc agactccctt ctattctaag aatacggaga aaataaacgg tggtcaagtt
     9301 tcaactgatt tttcaatcat tgtttatttt tctggagcat ttactgatga taagaatttc
     9361 atcatgaact tgaaaaatga taccactaaa atcaaaaaat aggtgggcaa acaataataa
     9421 acattttaat agtagatggt agcaagacca acacaggcgg attgaacatt taagagttga
     9481 taaaaaagaa aaatatcgaa gtatttgatt agcaactact tgtattcatt tttctattct
     9541 tacgtggtat ctttgaataa agcgaatcaa taagaaagag atggaagcag atgctaacga
     9601 aaaaagaact aatcgatcac atcaatgaaa catacaattt gtctccagaa tatattttta
     9661 aaaaatttcc tgattattgc gtattcagac atcaaaaaag caaaaaatgg tttggggtcc
     9721 tgatgtctgt tccagaagaa aaaatctatg atgatgcaag tgaccatagg atcatcaata
     9781 ttcttgattt gaaggtcgat cctgaacttg gcggaatttt gcgaggaaaa gaggggtatt
     9841 ttccagccta tcacatgaat aaagaacatt ggatctctat tgatctacgt aaaatagaaa
     9901 agctagatga actagcagat ttgattgatg gaagttttaa atcaacgcaa tgaatgtcta
     9961 ggacaataga gaagaggttg tgacagaaat gtttagcttc aagaaataag aaggaattta
    10021 cgaaaattgc ttctcaaatt tttgggaaat ttcggcttat tttctaagaa attacttctg
    10081 gaacaccgtt tgttcgaaaa aagagctgcg acaagaattt tgtcccagct cttttttttt
    10141 tattttccta aataaaattc ccgcatcgtt tttgcaatca ttttgatcaa aggatgatct
    10201 tctgtgaagc tagaagataa ataaattgtt cgtgtcaagg gcaaatcttt gatttcccgc
    10261 aagacgaaag gatcattttt atcgtaagac caagaaactt ttgggaaaaa gctaacagct
    10321 aatccttgct tgacaataga acgtaatgtc gcaggatcat ctgtcgtcga actgatcgtc
    10381 aattcgatac ttttcgcttc aagtgctgca tccagtgtat gacggaaggc gttgctcttt
    10441 gacagcatca agatgggggt gcgttggata tcttctaatt gcagatgttc ttttttagca
    10501 agaggatgtt tcttaggaat ggcgacataa acaggttctt tcatgatagg aatattggtc
    10561 atgccaggct tcatctccga agtgatgtac agtacaggat cggtaatatc atctggtaaa
    10621 tgatgttgca tgatcgttaa ttggatatca ggatgctttt ccataaaaat tcggaccaag
    10681 ttcggaatag aaacagaggc tacctctaaa tacaatatga cggaagtaac gatcttctca
    10741 ttgaccgaag acaataattt gattccttct tctaatgtgt ctaatgtctt gactgcatat
    10801 ggataaaatt cttctgcata aacggtcgtc tcaatgtttc tgccgctatg gtaaaacaaa
    10861 gggacaccta attcgttttc caaatgtttg atcgcacggc tcaaggcagg ttgagaaata
    10921 aataattctt tggctgcttg ggtaacattt tttgtttcga atactttttt aaaatactgc
    10981 agatgaagca atttcataat tatccatccc atccatacct ttttgttatg gttttttctt
    11041 tttttggcat tttttattat agcagaaatt gactaagata gcagtggaaa aagtaagtgc
    11101 tgaggaggcg aggctcatga gtacaggttt cttatttcct ggacaaggca gtcagaagtt
    11161 agggatgtta gcggatatcc ctgaaatgta catggaacgt ttagaaaatg tgacggggta
    11221 tcggttagtt gataaagaag aaaactatca agatacggtt tttatccagc tagcattact
    11281 gacaaaagca gtattttatt tagatgaaat ggaaaaaaac ggtattcagc ctgatattgc
    11341 agcaggacat tcgattggtg ctttttgtgc agctgttgct tgtcgatctt tgagatttga
    11401 gaatgcagcg gaactagtct atcaccgcgc agtcttaatg aaagaagctt atccaagcgg
    11461 ttatgcaatg ggcgtagtag taggtgtgac gagaagcgaa gctgaggaaa ttgtagcaaa
    11521 aacatttgat cctgaccatc cagtttatct gtcaaatgaa aattgtccga tgcaacatac
    11581 gttatctggg agtatcgaag gtttatccgt tacgttggca gaagccaaac ggcaccaagc
    11641 gcaaacagcg aagctgctga aagtccctgt gccatctcac tgtctcttga tgaatgatac
    11701 ggtcaagaaa ttcaagccgt atatgcagtc tgtccatcta tctgatgcta gatgtttgta
    11761 tataaaaaat acggatggaa gagccactca ggacgcagaa gagattcgta aagatttgtt
    11821 ggaaaaccta gctttccctg tgcagtggaa ccaaatgatg gacttggcaa aagaattagg
    11881 aatggatctg acgattgaat ttccccccgg caatactttg acaaagctga ttcatacaaa
    11941 atttggtgta gataaaggga tccgtacgat caatctagac caacatggta ttgatgacgc
    12001 cacgtttcta tatcaaaaat ggagatgact aattatggaa aaaccaaacc gcaactgggc
    12061 ttctaaacgc gaaaaaaaag cgcagcgatt ggaaaaaatc agcaacttgc tcaatggcaa
    12121 gtatgtagaa actgaaaaaa tcgtagaagt aatggaaaaa ctgattgctc ccggcgacaa
    12181 agttgtatta gaaggcgaca atcaaaaaca agcaagtttt ctttcagaaa gtcttgaaaa
    12241 agtagatgca gaaaaagtga atgatctgca tttgatcatg tctagtatct cacgaccaga
    12301 acatttgaat atttttgaaa aaggaatcgc aagcaagatc gatttctctt atgctggtgc
    12361 acaaagcttg cgtgtcgccc aaatgattga agacggcaca atgaaattag gtgatatcca
    12421 tacttatttg gaactttatg gacgcttatt catcgatttg attccaaatg ttgttttagt
    12481 agctgcagat aaagcagata aagacggcaa cttgtacaca ggtttcaata cggaagaaac
    12541 accaacaatc atcgaagctg cagctttcaa agatggtatc gtgatcgttc aagttaacga
    12601 agttgtcgat tctttaccac gtgtagatat ccctggtgac tgggtagacg ttgtcgtgaa
    12661 agcagataaa ccttatcaat tagaagcttt attcacacga gatcctcaaa acatcacaga
    12721 attgcaaatt ctaatggcaa tgatggcaat caaaggaatc tacgcagaac acggcgtaca
    12781 atctttgaat cacggtattg gattcaatac agcagctatc gaattgttac tgccaactta
    12841 tggtgaatca ctagggttaa aagggaaaat cgcgaagaac tgggtattga acccacatcc
    12901 aacgatgatt ccagccatcg aatcaggttg ggttgaaagc atccatagtt ttggtggcga
    12961 agttggtatg gaaaaatata ttgctgctcg cccagatgtc ttttttgtcg gtaaagacgg
    13021 cacgatgcgc agtaaccgaa cattatcaca agctgcagga caatatgcca tcgatatgtt
    13081 tatcggttca accttgcaat tagattacga aggaaactct tctaccgtaa caaaaggacg
    13141 tttatctggt tttggcggtg cgcctaacat gggacataat cctggcggtc gtcgtcattc
    13201 tacaccagca tggcaatcac ttcgcgacaa tgatgatcca ttagggaaag gccaaaaatt
    13261 agttgttcaa atggttgaaa catatggttc aaataaaaaa ccagtctttg tcgaagaatt
    13321 agatgcacta gctgttcaaa aacaagcagg cttagccaat gcaccagtga tgatttatag
    13381 cgaagatact acacatatcg tgacagaaga aggaattgct tatttgtaca aagcaaaaac
    13441 aaaagaagaa cgacaagcag caattgcagc aattggcggt gttacacctc ttggaatgac
    13501 tagcacgaaa gaatcactag atagcttgcg tgcagccggc atcgtgaaat taccagaaga
    13561 tctaggcatc aaacgtagtg aagcgaaacg ttcattatta gctgcacaaa ccatcgatga
    13621 tttgatcgaa tggtcagatg gcctgtatga accaccaatg aaattcagaa cttggtcata
    13681 aaattggcga gaaaaacctt ttgtcgccat ttacccagat ggaagaagaa agacgaataa
    13741 gcagaacgtg tatgctctgt tttacaaaaa ggggagagat ccatggaaca attagcttat
    13801 aaatatccaa cgaaacagcc gatcaataag aaaatccatg taggagtggt cggttcagga
    13861 gatttggaaa tcttgatgtt tccaacagaa aaaacagaat ctactgtaaa aatttgtaca
    13921 ggaagcgatg gcttcggcga agtatggaac aatgtattaa cgagattttt tgatcgttat
    13981 ccaatttcag cagatatcgt gatcaatgac ttcggtgcta caccaggagt tgtgtaccta
    14041 cgattgacgc aagcaatgga ggaactttca gatgaaaaat agttttgtag aattaaacgc
    14101 cagagaacga gtgaacgctc tacttgacaa tggacgtgaa ttgattgatc cattcgatca
    14161 aatcatcgcg ccaagtttac cagcacaagg aatcgtccct gaaagtgatg acgggatcgt
    14221 tgtcagccgt ggcacgatca atggcaaaca agcagtagta atcgctatgg aaggaaaatt
    14281 ccaaggtggc ggtatcggtg aagtttccgg tgcaaaaatc gttgctgcac ttgaacatgt
    14341 gttagaagaa aataaaaaag gaaatgagat ctttccagtc attgtactag atacaggtgg
    14401 tgttcgtttg caagaagcaa actacggact gttatctatt tcagaaatcg gaaatgtaat
    14461 cattgctcta aaacaatatg taccagtcat cggtttgatt ccaggacgtg taggatcatt
    14521 tggcggtatg tcgattactt cagcattaat gagttattta atcgcaacaa aaaaagcgag
    14581 agtcggtttg aatggaccag aagttattga acaagaagca ggcgttcgag aatttgattc
    14641 tagtgacaaa gacttgattt ggaatacatt aggatctcgt caacgggtag aatcaaaaat
    14701 cattgatgaa ttagtgactg atagtgtaga agcaatcaaa gctgctgtca tcgcagcaat
    14761 ggacgaacga aaagacacgc atcgcagtga aagctcagca ttttatttat cattacttga
    14821 ttctttagat ctttcaaaac caatggccat tgacgattac aatgaagcaa ttgctaacca
    14881 tcaagcaaaa tcaattgact taccagaagt agcagaagga aatgaaccag ctgcagcaag
    14941 tgttggttac gattggtttg cggcactaac tggcatgaaa aatccaactt cttctattag
    15001 tactgtgtat tatggtaaat caagtaaatt catagaagaa gccatcgtga catcaatcgt
    15061 acctgatcct aaaaacccga tgtaccgtgt acgaaacggt gaagttggtt tagttgaagg
    15121 tttccgcatg ggacaaattt tggatcacgt ttacgaagaa gataaagaca aaaaagtgaa
    15181 acggccaatc atcgttgtga tcgatgtacc aagccaagca tacggctata acgaagaatt
    15241 gatcggtatc catgtggctt tggctaatag tgctgctgct tatgcaaaac tccgtcaagc
    15301 tggacatcca gttattggat tgattgcagg aaatgctatt tcaggtgcat ttttagcaca
    15361 tggattacaa tcaagccgct tgattgcttt aaacaataaa gaaattaccg tccaagcgat
    15421 gtctaaagaa agtgctgcac gtgtgacaaa acgttcggtt gctgaaatcg aagcagctgc
    15481 tgaaaaagtt ccagctatcg cttacgatat caataattat acaaaactag gagcgatcta
    15541 tcatttccta gaagatatca ccgaccaagc tgcttcagaa caaaatgtca gcaaagtcat
    15601 cgaagcaatc aatgaagcca ttcaagatgt ccgcaataca aatgacacga tgctgacaaa
    15661 tcgatacaca aacgaaacag ccaaaacatt cggacgtgtc gaaacaaata aagtccgtgc
    15721 caaaatggac gaagaatggg atgcataaaa aatgattcaa gcacatgata ttgtcctttt
    15781 tgataaacat gaaatagaag ggttcacctt gcctgaatgg gtcgttctaa ctgaaaagat
    15841 gttcggaaca gttcgtcgtg cgtcgacgga tcacgcagaa atactaccga ttggtttacg
    15901 tggacctgcc cgcaatcaga gatggtcaac agaaatctca aaacatgccg tcaaacaagt
    15961 cattcatccg tgggacatca tcgaacaaga atcgtataga caagagaaaa tcatggatta
    16021 tcctgtttat cagcaatttc atgctgctag aaacatatta tcaggatgta aatggggaat
    16081 cggcggcagc ttaggatttg aattatcgac tgggattccg gcagtaaaag aaaccagtga
    16141 ttttgatttg ctgctgtacg caaattcacc gattgaattg ccaattcagg taattcaatc
    16201 gcatcctgct ttttttgagc aattcgatac ccaagtagtg actcggcaag gcggtttttc
    16261 actaaaagag tatttgagag agccagagaa aaaaattcta ttgaaaacag tcgaaggacc
    16321 taaattaacc aaagaacttt ggtaataaag aaaaagagag agaaagagat gtttgaaaac
    16381 aaaaaaatca aaatcaacgt aagtgacttc cagaaagtat cagctgtaac ggctgttatg
    16441 atgcaaacga tattgagctt tgctttggca aacagccaca gcgcaacaaa tacagctttt
    16501 cttggtacct tttacgtcat cgttaaatac acagcaccaa tgtttatttt tgcaattgtt
    16561 tataatatgg taaaaacaag tcagcatttg tcttattggg aatttttgaa agaaaagttt
    16621 tttgaactag tcgttccgta tcttctatgg acgacggctt atctttggct atttccagca
    16681 gtccaacaac aaactccgta tacggatact gcaagctttt tactgaaata tgtcacaggt
    16741 gatggagcgc ctcatttatg gtataccgtt atgatgctgc agatccaatt gttcatgccg
    16801 ttttttgttt ggctcggtta caaagtattt gctaataaaa aatatgtatg gccagtattg
    16861 attggttcaa cgattttata tgtcgcatgg tatctgttct atcaaacaca agtattgact
    16921 gggccttatc atgacagctg gtacttatta gatcggtttg ttgctagttt catgatttat
    16981 ggggtagccg catttgttta tcacgaaaaa gtctatcaat atttggacag agtacgttat
    17041 cttttcttac ctgttgcctt agttatcgcg ttcttttctg tccgttcatt gctcgcacat
    17101 ccaggagact tgagctttgc gaatgcacct tacttgaata cgatccaaag tttatacagt
    17161 ttagtcatca tctttgctgt cttcataggc gcttccaaaa tgattgtaaa cgattcgcca
    17221 aaattgccat tgttcaaatg gttgtctgtt tatgcttatc gtacgtattt agccaatgta
    17281 tttgttttcc aagtactgtt attattgttt aaagatagtt ggctgcagtt accttcagga
    17341 gtcatgattc ttgtagctta tctaatgacg gcaagttgtg catttgcttt aagctggtta
    17401 cttcatatca tttgggtagc tatcaaaaaa ggtttcagca agtaatagta ggaagaagga
    17461 gtgagtgtca tgataaatga cgaacgaaaa aaagcagtga ctcagcaact cgctgcgttg
    17521 gtagaggaag cattgatcgc agaagtaaca ttaagtccaa aacctggatt agtggatgca
    17581 ctggatgcgg gtgcacattc cgatatggac tatgctttat ttcttaagtc tgctaagacg
    17641 ctaactcctt tttttgaaga aatggctcaa atagcatggc atcatgctat tgatcaggag
    17701 ttaagagaaa gaattgctga gattgggcga aaagcggaaa aggcaatgct gattgctaca
    17761 aatggcatca atacccataa aggagcgatt tgggcaatgg ggctattgat cagtgtcatt
    17821 gcacaaaaaa tcagtatgga acagcctttt tctttatcga aaatacttga tgatgtcagc
    17881 aagcttgctg cttttcctga tacaaaatat cgtcataaaa aaaccactca tggtcatagg
    17941 gtgaagcaaa aatatggcgt aaatggtgca tatgaagaag cgatattagg gtatccgcat
    18001 atcaggcttg caattcaagc agctaaggag ctagataatg attcatatgt acagaaacaa
    18061 atgcatatgt tgttagtttt gatgagttcg ctagatgaca catgcgtatt gcatcgcagt
    18121 gatcagaaaa cgttagtgga aatgcaaaaa cttgctcatg ctgctagtca aagtgcttta
    18181 ccaaatcgtc catttacagc actgttgcac ttttgtcagg aaaaccaaat ctctcctgga
    18241 ggaagcgcag atctcttgtc tgcaagcttg ttcatgctga agatagagga attatggact
    18301 gccgacacat acatttgtcc gatgttactc aatgactaaa taagacaaaa ttgctaaaaa
    18361 aacagtttat gcatctggtt attgactctt ccttttagca aggctatcat aaaaatagtt
    18421 ttttgtaata aaagataacg aggaagacaa tgaaaaaaat caattattta gcaatcattt
    18481 tgttagtggg cctatgtttg tttgctctgc cgacccaagg attcgctgtc gcgggtggtc
    18541 atggtggagg tacatctagc ggtcgaagtt cgggcagagg ttcatttggt ggtcgttcat
    18601 atgggggcag ttattttggc agtcgttctt acggcagaga ttcttatggg tattatggtc
    18661 caggtacagg tccttctagt tatgagcaaa cgggatttct tgcattagct gttttgtcag
    18721 taggaggggt ttctgctttt tctatcgtca agaataaaag acaaaagaaa aggctgaaag
    18781 cggagatatt gtcccgcatg tcaggaacgg ctaaagaaaa gaaagcacag tttctggaaa
    18841 tccaaaagat attcatgaag atccaatccg cttgggaaga agaaactcct gaaaaagcca
    18901 gaagctgtta tacagacaag ctgtttgccg aacatcagaa agttctgcaa aaaaacaaag
    18961 cagacggtgt aagaaatcat actaaaaaaa taactattca ggaagtagga aattatcgac
    19021 agatccacga aaatagtttt gccatccgaa ttgatttcag atgctgtgat tattcagtcg
    19081 atcgaaaaga tgggcgattg atctcaggaa gtaagacaca acgacagtat ttttctcaga
    19141 tatggtattt tgatttttct gagaaagaaa aaagatggca agccgatttt attcagccga
    19201 ttatgctgga ttgacaggaa atatttatga acggagttgt gacaaaagcc ttgtcgcagc
    19261 tcctttttct gaataaacgg tgtgccagag atagcctatc ggcaataagg cacgattttg
    19321 taaaatatgg gaagcgattt ttcaaaatcg cttcttatcg ctcaaggctg aacatctctg
    19381 tcacaacctc tccactaaat ggtgtttaaa agcttgcatc tgcgataata agaacaacca
    19441 aacgaaaaat aggaagcact attttagttt agttgttctt attacttgat gcaggacagc
    19501 tttttcacaa cctctttttt gtatagatgg gcaaaatttt tgtaataata ggcgtatgtc
    19561 tttttcgttg aggagatgaa aaagcatatg gcttattttt tgtttgcttt gcttactttc
    19621 tttccacttc aagtggcaga aatatctact ttttattatg taagcggaac catatacttg
    19681 aaatcagaag gagatcattt ctatgaaaac aagaacaatt gaaatacttc acagaatcat
    19741 gacagcccaa tatccagtaa aagcaaaaca tctagcaagt gagttcaatg tcagtcagcg
    19801 gacgatccgt caggaagttc ttgaagcgaa tatctggttg agcagtaaaa agcttccgga
    19861 gatccgtaca attcgcaaca aaggtttttt gttgaagctg gaaaaggaaa agcaagctcg
    19921 attagaaact gaaataaaag atgtgaaaga cggctttttg aatcggcgcg aacgtatttt
    19981 tgatctagtt ttagccgttg cttacagccg aatcccgatc catctaaaca aaaaagaaga
    20041 agaatttcaa atctctaaaa gtacattaga tgaagatatg agacggttac gagcaacact
    20101 ggtcagttat gggatagaga tcgtcagtta tggaaagcaa ggactggttt ataaaggttc
    20161 agaacgatct atccgtacga tgatctatga ttttatcaat cagaatctgg gaatgaccga
    20221 cttctttatg aatcaagatg cacaattaac accggctcaa aaaatctttc tccattattt
    20281 tcctttggtg gaaatcaaaa aaatacagga gatctattcc aataagttgt tgaaacaaga
    20341 agatgatatt tataaaaaac aaatcctgct ttttacattg atctggatcc agcgaatcag
    20401 gaaacatgaa tttatctctg ccattaattg gaaaaataca gaaataaaag acagtagctt
    20461 ttctgcattt attgaccaca tgatcgagga atttcaattg gacaatgtcc cgcaagtaga
    20521 aagaaattat cttcgtttca ctattgagac gttcaatgct agagatatct ccaattcaat
    20581 cgagtgggta caagctcaat tactaacgat ccaattgatc caatttgtag agaacgagac
    20641 acacgtacct ttccatctaa aggaagaagc tttattcgaa aatctctata agcacatggc
    20701 tgcactgatt gtaagaatca agaatcatat ccaagtcatg aatccattga aagaaaacat
    20761 ccgaacaaat tatttcccaa tctaccgcgc agtcactcat tttgttccta cgatcgaaga
    20821 agtaattggt ggcaagatac tggaggacga gatagctttt cttgtcatcc acttttcgac
    20881 gatcgccagt gtaatcaagc gagacttgaa ttatgtttat aaatcagtcg ttgtctgtaa
    20941 ccatgggatg gctactggaa atctgttagc agagaatctg aaagaaaagt ttcctcagat
    21001 cgagattact gcagttttaa gttcaaaaga aatcgaatta gtagataaat tggatgtcaa
    21061 tctggttttc agcacctttc aattgaatta tcgaaataag ccgcttttag ttgtcgatcc
    21121 gattttaact gagacgaata tcttggttat agaagacttt ttgaaaagaa atgaagaact
    21181 acagcggtta gagcctaaca gtcatgaatc gacggagtta ttcactcaaa taatagatgt
    21241 catgaaagaa agtggtggcc gagtagatcg aatcatctat ggcaagttag aaaaattatt
    21301 cgaaacaaac aatttagaaa tcaacaaaag ggagatccag cctatgttaa aagatatttt
    21361 gacggacagc aatatcatga tcaaagaaaa agccactact tgggaggaat cgattgaact
    21421 agctgctaga ccattggtta aagaaaacat tgtcgaagag cgctatgtcg aagcgatgat
    21481 tcgtgcagtg aatgattacg gaccgtatat cgtgattggc aaacacatgg cactagccca
    21541 tgcccgacca gaagatgggg tcaataaatt aggagtcagc gtagctacga ttgagcaacc
    21601 aatcgatttc ggtaatcctg aaatggatcc agtaaagatt attttttgtt tggcagcagt
    21661 agactcctac tcgcatctga acatcatgaa agaactaatt gaattgatca acaacgaaga
    21721 gaagttggat cgattgattg aatgcacaaa tatcgaagat ttcaaacaac tgcttttttc
    21781 agtagttgag aaataataaa tgatacgggg gagaacaaag atgaacaaat tgaacatttt
    21841 attcgtatgc ggtgcaggtt taggaagtag ttttgcagca cagatgtctg ctgaggatgt
    21901 tttgaacaaa ttaggagtag aagccaaact ggaccattgt gatatttctt ccgcagtttc
    21961 catgaaacca gatgtgatca tcacagcaga aaattttcgt tcgcagttcg aaaagttcgc
    22021 agttgatcct aaaacggcga tgatctattt gaaaaatatt gtttcaaaaa atgaaattga
    22081 agaaaaaatc accccagttc tgcaagataa gggagttatc taaatacaaa aagaaaagag
    22141 ggtaaaaa
//
LOCUS       DAJSVD010000040        22028 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSVD010000040 DAJSVD010000000
VERSION     DAJSVD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.040"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(61..1371)
                     /locus_tag="ON498_001361"
     CDS             complement(61..1371)
                     /locus_tag="ON498_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3939622.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ON498_001362"
     CDS             complement(1383..2801)
                     /locus_tag="ON498_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939623.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ON498_001363"
     CDS             complement(3041..4609)
                     /locus_tag="ON498_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3939624.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ON498_001364"
     CDS             complement(4613..4894)
                     /locus_tag="ON498_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR3939625.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ON498_001365"
     CDS             complement(4891..5814)
                     /locus_tag="ON498_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR3939626.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ON498_001366"
     CDS             complement(5811..6689)
                     /locus_tag="ON498_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR3939627.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ON498_001367"
     CDS             complement(6676..8013)
                     /locus_tag="ON498_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3939628.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ON498_001368"
     CDS             complement(8016..9119)
                     /locus_tag="ON498_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3939629.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ON498_001369"
     CDS             complement(9193..10701)
                     /locus_tag="ON498_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3939630.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ON498_001370"
     CDS             complement(10667..11359)
                     /locus_tag="ON498_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3939631.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ON498_001371"
     CDS             complement(11381..12355)
                     /locus_tag="ON498_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3939632.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ON498_001372"
     CDS             12655..13527
                     /locus_tag="ON498_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939633.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ON498_001373"
     CDS             complement(14109..16307)
                     /locus_tag="ON498_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939634.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ON498_001374"
     CDS             16539..17531
                     /locus_tag="ON498_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3939635.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ON498_001375"
     CDS             17591..19012
                     /locus_tag="ON498_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3939636.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ON498_001376"
     CDS             19005..20504
                     /locus_tag="ON498_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3939637.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ON498_001377"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ON498_001377"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3939638.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAJSVD010000041        21911 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSVD010000041 DAJSVD010000000
VERSION     DAJSVD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.041"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            226..924
                     /locus_tag="ON498_001378"
     CDS             226..924
                     /locus_tag="ON498_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCR3939639.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESQAYEKISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            1111..1452
                     /locus_tag="ON498_001379"
     CDS             1111..1452
                     /locus_tag="ON498_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939640.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1445..2116
                     /locus_tag="ON498_001380"
     CDS             1445..2116
                     /locus_tag="ON498_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3939641.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2122..2808
                     /locus_tag="ON498_001381"
     CDS             2122..2808
                     /locus_tag="ON498_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939642.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIERLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2815..3666
                     /locus_tag="ON498_001382"
     CDS             2815..3666
                     /locus_tag="ON498_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR3939643.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            3678..3947
                     /locus_tag="ON498_001383"
     CDS             3678..3947
                     /locus_tag="ON498_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939644.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3952..4116
                     /locus_tag="ON498_001384"
     CDS             3952..4116
                     /locus_tag="ON498_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939645.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            4109..4411
                     /locus_tag="ON498_001385"
     CDS             4109..4411
                     /locus_tag="ON498_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939646.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            4408..4719
                     /locus_tag="ON498_001386"
     CDS             4408..4719
                     /locus_tag="ON498_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR3939647.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            4716..4934
                     /locus_tag="ON498_001387"
     CDS             4716..4934
                     /locus_tag="ON498_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HCR3939648.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            4945..5760
                     /locus_tag="ON498_001388"
     CDS             4945..5760
                     /locus_tag="ON498_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR3939649.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            5899..6255
                     /locus_tag="ON498_001389"
     CDS             5899..6255
                     /locus_tag="ON498_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3939650.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            6242..6460
                     /locus_tag="ON498_001390"
     CDS             6242..6460
                     /locus_tag="ON498_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939651.1"
                     /translation="MDLYDIESGATVSVESDHLIISFADERQIIIWVVDDMLYPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            6457..6855
                     /locus_tag="ON498_001391"
     CDS             6457..6855
                     /locus_tag="ON498_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR3939652.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            6852..7190
                     /locus_tag="ON498_001392"
     CDS             6852..7190
                     /locus_tag="ON498_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939653.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            7187..7417
                     /locus_tag="ON498_001393"
     CDS             7187..7417
                     /locus_tag="ON498_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939654.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            7424..7627
                     /locus_tag="ON498_001394"
     CDS             7424..7627
                     /locus_tag="ON498_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCR3939655.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            7624..7917
                     /locus_tag="ON498_001395"
     CDS             7624..7917
                     /locus_tag="ON498_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939656.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHIYNHSAIIKIENTMECDRSLAKSKENLAVARLVDMEVIKS"
     gene            8158..8571
                     /locus_tag="ON498_001396"
     CDS             8158..8571
                     /locus_tag="ON498_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3939657.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            8929..9291
                     /locus_tag="ON498_001397"
     CDS             8929..9291
                     /locus_tag="ON498_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939658.1"
                     /translation="MYLRQNVFTKKDIENQINGFIQKLESANSYLINKELNKAYDQWK
                     EVYDELKSIQNETKLVRTEKKNTNSFFFDGYAILMLETVAKQNIKASKKELSDNIDNA
                     LAELRYYVMQIKDARITH"
     gene            9329..9496
                     /locus_tag="ON498_001398"
     CDS             9329..9496
                     /locus_tag="ON498_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939659.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVREYRRPIKSDTYKRKENNKV
                     IPLYGKRTAKH"
     gene            9522..9866
                     /locus_tag="ON498_001399"
     CDS             9522..9866
                     /locus_tag="ON498_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3939660.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEAKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9871..10152
                     /locus_tag="ON498_001400"
     CDS             9871..10152
                     /locus_tag="ON498_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939661.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            10255..10569
                     /locus_tag="ON498_001401"
     CDS             10255..10569
                     /locus_tag="ON498_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3939662.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10547..12241
                     /locus_tag="ON498_001402"
     CDS             10547..12241
                     /locus_tag="ON498_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3939663.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            12261..13439
                     /locus_tag="ON498_001403"
     CDS             12261..13439
                     /locus_tag="ON498_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3939664.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13402..14088
                     /locus_tag="ON498_001404"
     CDS             13402..14088
                     /locus_tag="ON498_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3939665.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            14088..15248
                     /locus_tag="ON498_001405"
     CDS             14088..15248
                     /locus_tag="ON498_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3939666.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            15258..16133
                     /locus_tag="ON498_001406"
     CDS             15258..16133
                     /locus_tag="ON498_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939667.1"
                     /translation="MKYILCQPAINRFKWEFEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            16130..16441
                     /locus_tag="ON498_001407"
     CDS             16130..16441
                     /locus_tag="ON498_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939668.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16431..16784
                     /locus_tag="ON498_001408"
     CDS             16431..16784
                     /locus_tag="ON498_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3939669.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16774..17175
                     /locus_tag="ON498_001409"
     CDS             16774..17175
                     /locus_tag="ON498_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939670.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            17168..17572
                     /locus_tag="ON498_001410"
     CDS             17168..17572
                     /locus_tag="ON498_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939671.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17584..18192
                     /locus_tag="ON498_001411"
     CDS             17584..18192
                     /locus_tag="ON498_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3939672.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGAAEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     SEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            18212..18574
                     /locus_tag="ON498_001412"
     CDS             18212..18574
                     /locus_tag="ON498_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939673.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18577..18759
                     /locus_tag="ON498_001413"
     CDS             18577..18759
                     /locus_tag="ON498_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939674.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18777..>21911
                     /locus_tag="ON498_001414"
     CDS             18777..>21911
                     /locus_tag="ON498_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3939675.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSV"
BASE COUNT         8024 a         3206 c         4687 g         5994 t
ORIGIN      
        1 atattagtcc agttttaaaa aaagagaagt aatctatgtg ggcaaattct gttaatatgc
       61 ttttttgatt taacacaatc aaattactgt tggataaaaa tgacagtaag gaaagagttg
      121 ctccgattac taaataaaaa atagttgtta acaatattat attatcaatt tttctgctca
      181 tgttttcacc ccaccaggat tatatcaaaa aggagagaag aaataatgca agaattagta
      241 attttgaaaa ataaagaagc tgtgactacg agtttgcaag ttgcagaaag ttttgagaaa
      301 aagcatcaac atgttttaag agatattgat gcactgaaaa aagatgtgtc caattttgga
      361 cagatgtttt ctgaaggtaa tgagccagat tcctacggaa gaaatcgacg agttttcttc
      421 attagcagag atggattttt cttgttagct atgggattta caggaaagaa agctatttac
      481 ttcaaacaaa aatacattga agcattcaac gaaatggaag atgttattcg caagaatact
      541 gttcctcaaa caattgaaga catgatgatc taccaactag aagaaatgaa agatgttaaa
      601 aaagatgtct ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt
      661 aagcaaaaag gaaatatgaa agttatggaa gttctaggag gcaaagaaag ccaagcttat
      721 gaaaaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca
      781 atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa
      841 atgtggttac cagaaactgc gatccgcatg gaaatcgatc aactgaacag acaacaaaga
      901 cttttcggtg atgaaaatga atagagctga agcgctaaga atcgggacgg taattgctaa
      961 tcgctggtgg agacacaata aacacaataa accaatcatc ctaagccaac aacatattga
     1021 caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa
     1081 agaaaacaca tcataaggag attttagcat atggaaaaag aactttccac tctagatcaa
     1141 tatttgactg atcctagttg gggcaaatcg aatatcaagg aaacaaataa tcgaaaaatc
     1201 agacgaaatc ttttgacgga tgaagaacta gcatgtgatc aagacgattt gggaaatttt
     1261 gtgagtattt gggatcatgt ctatcttatc catttatcga ggaagtccaa aaaacctgaa
     1321 tatatctatg tcatcgaaga tggcttgatt gatgcgctag aagagtatga cagagataac
     1381 ttgattgata tctcttatta cggatcaggt aagaaataca ttgctgaaat ggaggcagaa
     1441 tttgatgagt gaaaacaaag ggacaacgac ttttgaaaaa ctttttagtc gcaagttaaa
     1501 taaaatactc aagaaaaaag gaaattttga ttatttatct tgggctcacg catgggagat
     1561 tatgaaaaag aatgatccac aggcaacggt aactattaat gagtataaac actacagagt
     1621 tgtttctgga actcatcaag actttcttgt tgaggaatat aaaccttttc ttatggatga
     1681 aactgggact tatgtatctg tctcagtaac ggttaaagga cacacggaaa ccgaattatt
     1741 tcctgtttta gattatcgaa accaaccagt tgttaagcca aatgctatgc aaatcaataa
     1801 ctcattgaag cgatgctttg tgaaagcatt ggctctacac ggactgggat tatatgtatt
     1861 tcaaggggaa gatattccaa caccacctag aatcgataca aagaaattaa acatgctaga
     1921 gacgattcta gaagctttca atgagcagat gggtaaagat atgaccaaaa ccttaatcga
     1981 atatgttaat gagcagacag ataaattagg gctcttagct gataacgttg aaactattga
     2041 acagttaagc tatgagcaat gtgccttgat ggagcgagca atagcagcta agagaaaaga
     2101 attagataag aagtgatatg agtgtttaaa ccattaatcg attcatattc agcggttctg
     2161 aaaaagttca aaggaaaaga cataggtgca actattaatg aagaagtgaa catcgaacgt
     2221 ttgaagacga tgtacgacgg atatgatggc gatcggatta ttgaagttcg atttattgat
     2281 cctagacgtt tcactgtaca gcaacgaaac ttcatctatg cgctcatagg cgatattttc
     2341 atcgatacag gcatgccaac ggacttctgg aaggaattct tctactttcg ttttgaaggt
     2401 gtcacagggc gcaaaataag cctgaaagac gaatcgaata caactgtgag tgatgccaat
     2461 gtcttagcaa atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc
     2521 tatgagattt taccagcgaa tcaagaatat tacttctaca aatgcattac aaaaagagtc
     2581 tgctgcatct gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga
     2641 cgaaagcgca aagaagttga tcattcagag tacacgtttg cagcactctg cagaatccat
     2701 cacacggaga agcacaaaat aggtgtgatt aatttcaaaa ataagtatca aatcaaaggg
     2761 atcaagttaa accaggaaac aattaagaaa cttagaatag gagggtaaaa aatattgtct
     2821 gacaacaaac gctactacta tttaaaacta aaagagaatt ttttcgatag tgacgagatg
     2881 gttctcttag aaagtatgcc agatggctat atttattcta atattcttct caaactttat
     2941 ttaagaagtc taaaacacga aggtaagttg atgtttaatg acaggattcc atttaactct
     3001 acaatgctcg caactattac aagacattct gtaggagtcg tagaaaaagc ggtacaaatt
     3061 ttccgtgatt tgcagcttat tgacgtatta gataacggag caatttatat gtctgatata
     3121 caaagtttca ttggaaaatc ctcaactgaa gcagatagaa aaagagaata cagaaagaaa
     3181 atagaagagg caaaacggaa tttaataact ggaggacaag tgtcggacaa atgtccggac
     3241 aaaactacac cagagttaga gatagagtta gagaaagata tagagttaga gaaagatata
     3301 gagaatgtaa cgccttcgaa aaaatcgaag gctaagcccg tccgtcataa atacggagag
     3361 tataaaaatg ttcttttgtc agatgagcag atggagaaac tcaaaataga attccctaat
     3421 gactaccaag agcgaataga acggctatct gagtattgtg aatcatctgg taagacttat
     3481 aaaaactatt tggcaactat tcgaagctgg gcaagaaaag aaaaaagtga atctaagaac
     3541 gcaagcagtg gatacaagcg cacaggaaga cgagagaagc ttcctgagtg ggcaatcgac
     3601 caagaagcct atctcaagaa aaaagcgcta gaacgagcta atagacaatc aaaagcacca
     3661 ttctaagagg tgggaaattg aaaatcgatt atctagaact aattaatgaa atagcaaagt
     3721 ataaaactgg tgaggaaata gaaatcctga gagacgtata tgatcaactc gaagaagctg
     3781 gaatcgaagg aattaagaat gatcgttcga gttggagtaa gctcagatac tatttcgcac
     3841 tttatatcga tacaacacaa ttaagaaatt tagcatatac caaattacta tttgttgatt
     3901 gtgttaaagg cttgcaaaaa catcttaatg aacttgagca ggtgtaatca gatggaccta
     3961 aagacattta cagcacagat cgaactaatg catcaagaag ctttaagaca aagcgcctcg
     4021 tacgaagaca agtggctcaa cacgttccat ggtggacgtg agagcgcact tgatcaagta
     4081 ctcaaattat tgaaagggga acgtcgggat ggataagaaa gcggcaatga aacgaatcat
     4141 cgaactgaca cattctgaga attggcaaga agacaaagaa atagttacag aagtccaaaa
     4201 gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat
     4261 ttggcatggt gatcgaattc tagtaacagg tactgctgaa cagttatctg aaattactgg
     4321 tctgagcaaa aacatcatct gggatagagc aaggagctta tggattgatt caaaaggacg
     4381 acaatttagg tatgtggagg agaaaaaatg aacgaactaa tcacaaaagt agagcagtgg
     4441 gctaaagaca agggactgga tcaagcagat ccaaaagcac agtttttgaa agtagctgag
     4501 gaattcgggg aaatcgcttc agcaatggca agaagtaatg atgagctatt taaagatagc
     4561 gtaggagacg taatcgtcac gttgattatc ctttccatgc aaaaagggac aaacgtacaa
     4621 gagtgtttag aaatggcata caacgaaatc aaagggcgca caggaaaaat ggtagatggt
     4681 gtattcgtga agtcgagtga tttggaggac agcaaatgat aggtttaaca atattttggt
     4741 gtgcgattgg tggtttacta gcatttctag gatactacct catctgtgaa agtagaaaag
     4801 gaagaaaata tacagtgaca ggtattgtcc tgttaattat cggaattcta gttgcagttg
     4861 gagcagaact gaatctttat gtaaatggta cacaagaaga tttggtgcga tttatgtttt
     4921 ggatgaagaa ttaggaggaa aaaaatgaac gaaaacaaat taatcaaatt aggtgtagca
     4981 ggagcagtaa tcgtaggtat tggagttatc ggaggattta agttcttcga aaaaatcgat
     5041 aatggatatg tgggtgtgcg ctattcaatg aacggcggta tcaaagatga agcactgacg
     5101 caaggtgtga aatttgtagg gattgacaaa gtgatccaat atccaattcg cttgcaaact
     5161 atccaatcaa aaaatatttc agtatctaca agcgacggca aaaagacaac gattgatatc
     5221 aaatatgact acaaagttga ttcaactaaa gcagcaaaaa tgtacaagga atttgggaat
     5281 atcacttcgg aagatatcga aagtggatgg ttaaaatcta agcttcaaaa ggtcgctcgt
     5341 gaagtttatg cgaaatatag tctgcttgat gtcctttcag gagattcctc taaagttgaa
     5401 gctgaggtat taacgaactt tgctaaatca gttgaatcta aagggtttga agtcgaagac
     5461 gtaacacttg gtgttccaga tgtcgataaa gaaacacaaa aatcaatcga tgcgatcatt
     5521 cgagctggtc aagaaaatga aaaagcgaag ctagatgcag aaactgcaaa aactcaagct
     5581 gatagtgaag cttacaagaa aacaaaagct gcagaagcag aggcagaatc taatcgcaaa
     5641 gtcgccgaat cagtaacaga caatttgatt cgttatgaag aagctcaagc tcgcaaaaag
     5701 catggatggg taacagtaaa tggagcagat actgtagtta cggatgaagc aggcaaataa
     5761 tatgggattc tttatggcta aaattctctt gttcttaggt ttagtaggag cagcatatct
     5821 cgtgtatgcg ctcctttccc aaactgatga caaagaagat gacaacaacg atgaaatgaa
     5881 attttaggag gagaaataat ggatctcatt acacaataca gtgatatcat cctcaagaaa
     5941 atcatggcaa agattcagaa agataaaaaa gcaaaagaac gagcggaatt agttaagttg
     6001 gaaatggctg aaacaggagt aggagtgcga agtagcaggc attggaaagc agcagcaaac
     6061 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     6121 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca aatttgtaga gaagtataaa
     6181 gaaatattag aagagtattt gaggaggaca gcgaatgata aaaaagctca ttcaattcag
     6241 catggattta tatgatatcg aatcaggagc aacagtatct gtggagtctg atcatttaat
     6301 cataagtttt gctgacgaac gtcaaattat catatgggta gttgacgata tgctatatcc
     6361 agaaattgta catgattttg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt
     6421 aatggaattg attgaaaaat acgaggagga cagcaaatga taccgaagtt tagagcgtgg
     6481 gataagcgag aaaacacaat gagagatgta gctgtcttac attttactaa aaacggcaaa
     6541 acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac
     6601 atcgacctca tgcaatccac aggaatgaaa gataagaatg gtgtggagat atttgaaggg
     6661 gatgtagtaa ttacatcaaa aggagcgatt ggatatgttg cgtatcttca acaagaagcc
     6721 ggctttgtag ttgtactaaa gaaaagtgat tatagattag gccataggaa tactggcgaa
     6781 agttatgatg tagcaactgc tcatgaagtc atcggaaata tatacgagaa tagcgagtta
     6841 ttggaggtag agtgattatc ttagctactg actacatcga ttctttaaaa gatgaaaatg
     6901 ggaatgtccc agcattgcta aattgtggaa ttaaaagcaa gaatagaatc attttgttac
     6961 ggatatcggc tgatttagca aaatatcttt atctgagcaa gacagcagta tattacactg
     7021 cggctacacg ttatcagtac aagggaaaaa caatcagtca ggattattat gatcgtttaa
     7081 tcagtcctga catgcatgga aaatcaaaat cagcgatcaa gttatttggc gcaatagaga
     7141 tatatgccga tgattttcca aatttatggt tgaaggagga agcggaatga aatacgaaat
     7201 accactaagt gaagcgggca ttcaagcaat tatcaatggt cgggaggtta ataaaaaact
     7261 tcctgatggt actgaattag tcatcagaca aagttatttg aaagatatgg cagctccagt
     7321 attaattgat cgttttaacg tgactgattc tgtggtagag aaccacttga aagaatttcg
     7381 atcaagtata aacgacactt tcagattagg gagttgattg acaatgaaca ccagacaccg
     7441 cagagtagca aaactaagaa aacaggaact gaatgtacta aagacaaagt ttgaaaaaga
     7501 atatggaatt tcagcagaag aaacatataa agtggcaagt cagtttgttg ctgatgcgag
     7561 tgagactatt cgtaagtttg ggatttcgat attaaatgat gatcgtaaat gggaggcaga
     7621 aagatgaaac taaaagacgg attttacact gatgcttatg gaattggtgg tttgatgatg
     7681 gatctaccaa ctaagaatcc tataaagcaa gaggaatcag aaattaaagt cggtgacatg
     7741 gttcgctgcg aagcagaagg gttcatctat ccatttcgtg gatatgtaga gcatatctat
     7801 aaccattcag ctatcatcaa gattgaaaat acgatggagt gtgatagatc actagctaag
     7861 agcaaagaga atctagcagt ggctcgattg gtggatatgg aggtaattaa atcatgatta
     7921 caaatatcat aagtatcaca gcaatagtta ttctattttt gatagttgtt tctctaagca
     7981 atgaaacaac taatctcaaa aaaagaaaat cataaactaa aaatcatgct gaaagacaca
     8041 aaatatgctg aaattgtcga tctattatct gatgacaaat aaaaaagccg gatcgctccg
     8101 accgttctaa taaattccac aagtttatta tatcacataa aggagcggtt tgacttgatg
     8161 caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag
     8221 aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgcctatc
     8281 ataaccgata tgccgaaggc accgaagcac ggcaataagg cagaggacgc aatcattcag
     8341 atgatggaca tagaagcaga gagagatgcg attttagcgg ctttgatggc tcttagtctg
     8401 attagtcgtc agatactcta ctacagcttc tgtgtgccag atagcttctc aaattacaga
     8461 attagccgtg aagtgggtta ttcagaaaga agcatacaac ggatgaaatc agaagctcta
     8521 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata atttggcggt
     8581 tttttggcgg aatgatggcg gttttgcaac gttttgcagt gatattatga tagtgtcgaa
     8641 agattagtga taggtctgag acaaaataaa acgcaaggga ggaaatctcc ctcatcgttg
     8701 taattaagct tcgatagaca gcagcaaata aactaaagga tgtggggttc agctcctaca
     8761 gttagttcat gtgttgctgt ctattaattg caactctttc ggttttattg agtatttact
     8821 caacttaaaa aaactgttgt ttgtagatgt aagttgtgcc tattatacat agtaaaaaat
     8881 gatataataa aaatattacg aaaggggtga gagtgcttgg aagacaaaat gtatttaaga
     8941 caaaatgttt tcacaaaaaa agatattgaa aatcaaatta atggatttat acagaaatta
     9001 gaaagtgcaa attcttattt gataaataag gaactaaata aagcttatga ccagtggaaa
     9061 gaagtatatg atgagttgaa atcgattcaa aatgaaacaa aattagtacg gacggagaaa
     9121 aagaatacaa atagtttctt ttttgatgga tatgctatat tgatgttgga aactgtagca
     9181 aaacaaaata taaaagcttc caaaaaagaa ctatctgaca atattgataa cgcattagcc
     9241 gaattaaggt attacgttat gcaaataaaa gatgctagga tcactcattg agtggtcttt
     9301 ttattttgca caaaggaggt taacaacaat gtatagacca caatacttag aacagaagta
     9361 tgaagtaatc actgtgcaaa acggtaatgg tgagatagta cgagagtata gaagaccaat
     9421 aaagagcgat acatataaac gaaaggaaaa caataaagtt attccattgt atggcaaaag
     9481 aacagctaag cattaaataa gattgcgaaa ggagacggaa catgaccgag gaattctata
     9541 gatggctatt acagttgata agagaagatc gtttggttaa gttctatcag tctcctaaat
     9601 ggcgcaggct tagagagaaa gcgatgaaac gagatcacta tgaatgccaa gagtgtagaa
     9661 gactaggtaa gtatcataga gtagagaacg ttcatcatat aaaggaagcc aaggatagac
     9721 ctgacttagc tttagattta gataatctta tttgtttatg tgttgaacat cataatgaag
     9781 ttcatggcag atatcttaca gcattagata aacaagagaa gaagatagaa agctttgcta
     9841 acttcgatgc aagtgaaagg tggtaagtgc atgatcatca atgataatgg cagagagtat
     9901 gatacagaaa agattgaaga gtattcatct tatactcagg gattaattaa acgtttgata
     9961 tacgttcgct atgtaggtat tagggatctg ttatcagata actgttgtag taaatacaaa
    10021 gtgaatcaag taagagaagc gttgaataaa gataataacg tcgaaagaat aaaaaatgtt
    10081 tttggatata gtattgaaga gattaattat tacattgact tcgctgaagc tttcattccg
    10141 atggtgagat aaccccccct taaaataaat cgcaaatttt ttgggggtga tgaaacggag
    10201 ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg aaaggagggc taaaatgttt
    10261 aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac gccgctcttt aataagccaa
    10321 ttggaaagtc agaaaacaaa tattgagcca ttcttagata atgttgatcg ttatatcagt
    10381 ttatgggaaa cggcgatatc actggaagaa gatatatccg agaacggcat tagactggag
    10441 aatggtaaaa agaatgaatc agtagcgttg cttgtttctg tcaacaaaca aatgggattg
    10501 atgttggata aacttgccat tactcctgaa ttggtaggtg aagcaaatga atcaattcct
    10561 gagttataag catattgaaa attggttcaa agctatagaa gaaggcacta tcaaggtatg
    10621 caaagagcaa ttattgctaa aaaattatct agaagaaaga gtctttacta gagaagatat
    10681 ttacttcgat aagcagatgg tagaggattc aatcaatata ccagcacaat actttccatt
    10741 cgaattaatt ccgtgggaaa aatttctaca atgttttatt tatggtgttc gatggaaaaa
    10801 agataaaaca ctagtgttca atagatatct ttcattaatg ggacgtggta atggtaaaac
    10861 tggttttgct tcttggaaca acttctttct actaaccgct aaacacggta ttaaaaatta
    10921 tgatattgat atctatgcca ataatgaaag ccaagcaaag actagttttg atgatgtatt
    10981 taaagtaatt aaagatcatc ctgatttaga taaaaaagta tttaaagcta cgaaggaagt
    11041 tattcaaaat atcgctacaa acagcaaact tcgttataac acggcaaatg ctagaacaaa
    11101 agatgggaag cgaccaggtg caaaccgctt tgatgaaatt cacgaaaatg aagattattc
    11161 aatgataaat gtggctactt ctggtggtgg taaaattcga gattatagag aattttatga
    11221 tacaactaat ggtcatgttc gtggtggtcc gcttgatgac attatagaag aatcaaaaat
    11281 gattctttct ggagaacttg gaattgacaa ggatggagca gaattttcta gtttgtttcc
    11341 atttatttgt cgcttggata acgataatga agttgatgat cccgacatgt gggaaaaagc
    11401 ttgtccaact attaattaca atgcagattt aaaacggaaa atgtttcaag aatactctca
    11461 aatgcaacgt aatgctggtt taagacttac gttcatgacc aaacgaatga acagacctat
    11521 ggaagataca cgatttgctg ttgcttcata tgatgatgtt ctgcatacga aagaaaaaga
    11581 atttcctgaa aaaatggatg aagtgatagg aacagtcgat tttgctgata gacgagattt
    11641 tgccagcgtt gggttgctag gaaaatacga taaagatgtt tattttacac aacatacttt
    11701 tatccacgaa tcagcccttc gattacaaaa catcaaacga gaggttatag atatttctat
    11761 agatcaagga aaatcacaga tcgttcatgg aaaaaatata gaagctgatt atattgtagg
    11821 ttggtttctt gaaatgagta ataaatatta tattaaaaaa atcgctatgg atatgtaccg
    11881 tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt actgtggaaa ttgttcgaag
    11941 cggatctgtt acacatggta tgttaaaaga tctggttgat gaccttttta ttaatcaacg
    12001 tttatttttt ggtgacgatg cgattatgcg ttggtattgc atgaatgtat atgaagagca
    12061 tatttctaat ggaaatatac gctatgaaaa aatagaacct gaaactagaa aaacggatgg
    12121 ctttttttca ttccttcatg gtttgaattt tttagatgat atttatgatt ctgctcctgt
    12181 aacagtcaca aatagctcag tagaaaatac aggaactgga tttactcctc tagtattcta
    12241 acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc ggtaggatac ttcacaaaaa
    12301 aagcaacggt tcctttagaa gaatactttt gtaaattgca agttgatttt gtgtatcgaa
    12361 aatttgcgat tgaaacttgt attgatttga ttgcaaatgc gatgagtaaa gcggaattca
    12421 agtcatatga agatggaaaa aataaaaaga atgatcttta ctataggctg aatgtagctc
    12481 ctaataagaa aaataatgca acagaattta gaaaaaaact gatcaggaga ttaatattct
    12541 acaatgaagt attgatcgtt tctccgtcta ataattctag cgaaatattt attgcggata
    12601 gttgggatgt cacagaatat gcattgaaag atgatgtgtt ttctcaagtg caaattaaca
    12661 acatagtcct tgatagagaa tttctagaaa gtgatgttat ctatataaaa tacgcagatc
    12721 aacaaattag gcaactagtc gatgcgtatt atcaagcgta tgggaaactc atttctagtg
    12781 ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt actgaaaggg aatttattcc
    12841 gaccgcaaga caatacaaca caagatcaaa tcaataaaat gatgacatca caatttaagg
    12901 cttttatgga agctgataat gcaggtgcgg tatttcaatt acaaaatgag tacacattag
    12961 aagatttcag cggaaacttc caaagcaatt caagagatat aaaaaactta atagacgaca
    13021 tctttgagat gacagcagca gcgtttcacg ttccgaaaaa cctactaaag ggagacatga
    13081 gtgggttatc ggatcaagtg gacgcttttt taatgttcga aatcataccg attgctgaac
    13141 ttattcagga tgcgtttaac gctagtctct atgaagtaga agaatacttg tcagggaatt
    13201 ttgtacgtgt ggatacaact atgatcaaga ttactagctt caaagatttg gttgacgcta
    13261 ttgatgtagg cattagaaat ggagtattta caatcaacga aggaagagaa cgcgttggaa
    13321 atgatcgctc tgataaggcg atggcagatg aaatatttat aactaaaaac aatcaacaag
    13381 tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat ttctagcagt aaagaatgaa
    13441 ggcgcagtac cgcaaatttt tattcaggga tttattggtt ctagttggtt ctttgaaggg
    13501 aatactgaca agggaatcaa aaatattttg gatagtctag gtgatcaaga agaaattgaa
    13561 gtagtaatta attcaaacgg tggagacgta tttcaaggga ttgctattgg gaacttactt
    13621 aagtcaaata aagcaaaagt taacgttgtg attaacggct tagccgctag tgctgcttca
    13681 attatcgcaa tggctggcga tactataaaa atctacaaca atgcacaatt gatgattcac
    13741 cgcgcttcca catacggaga aggaaatgtc gatgacttcc gtacgattgc tgaccaactg
    13801 gaatcaattg ataaatcggt aaaggcttca tataaaacac gattcaatgg cacagatgaa
    13861 gcattgcaag aacttcttga aaaagaatcg tttatggatg cagaaacagc tttgagttat
    13921 ggattggtcg atgaaattat cgatgcagaa aatagctcag gtactgaagc taaaaaagaa
    13981 caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa gagcagaaaa aattgctgca
    14041 tttacagcag cattaaataa aacatttgga caaggagatg taaaataatg acagttaaaa
    14101 atttaaaagg tgtaacagct gcaagcgacc aattgatgaa agcttttaaa gatggtaacg
    14161 aagaatcttt tagcgcagct atggtaagct tatctaagga aattcaggat aaaattttag
    14221 aagaagcaac agcaaaaaat caagatcaat tagtattaat gaaccgtggt cagcgtgtat
    14281 taactacaca agaaacaaaa ttctataacg aagtagtgaa aaacgaaggt tttgcagggg
    14341 tcgaagaatt agtgccagct actgtatttg aacgtgtatt tgaagattta gaacaatctc
    14401 atccactatt gcaaaaaatt acttttgtta acacaactgg tgtaacagaa tggattgtgt
    14461 cacgtggagt caatccagca tggtggggta aactttgcga agctgttaaa aaagttttag
    14521 ataatggctt tgatgtaatt aacatgaagc agttcaagct atcaggttat attcctgtat
    14581 gtaaggcaat gcttgattta ggtccagtat ggttagatcg ttatgtccgt actgttttag
    14641 tagaatcatt gagaattgca ttagaacaag caattgttga tggtactggt aaagatatgc
    14701 cagtcggaat gatgcgtgac atgagcaaac aaactagcgg agaatatgct gaaaaaaaag
    14761 cagaacctat tacagcttta gatgctgtaa ctatgggcgg tttgatggcg cgactatcaa
    14821 aattcaatat cgaaggtgtg aatgatccga tttatcgtaa tgtgaatcct tctgatgtgg
    14881 tcctaattgt gaatccaact gattactggt ctaaagtatt cccagctaag actgtactaa
    14941 ctgctaatgg agaatacgta caagtattgc cagtaccagt ttcagatttg cagtcaacgg
    15001 ctgtgccaga aggaaaagca gttattgggg tagcctcaga ttacttcatg ggtgtaggat
    15061 ctacactaaa aattgaagct tcagatgaat accattttgt tgaagacgaa cgcatttatc
    15121 tagctaaaca atatgcaaac ggtcaaccta aacgtaacga tagtttcatt gtattagata
    15181 ttagcgcttt gggaactact actacaacta caaaaccaac aaccacaaca actacaacac
    15241 aagcgtaggt gatcagaatg aagtatattc tttgtcagcc ggcaatcaat cggtttaaat
    15301 gggagtttga agtttgttta actaatctga agaaactagg aatcaaagat atcgtattgc
    15361 ttttcagcag acacgatgat cagattccta ttttttttga gaaggaatat ggtgttgaag
    15421 ttcatgtgta cgatgatctg cgggacgaca aagagtatat tccttcgatt aaaccatatt
    15481 tatggtggaa atatttagaa gaagatcatt cgcgtgagga cgaccgatat ttctatatcg
    15541 attcggatgt cattttcaat aaaagaatta atttgcgcaa attgccttct aaagatgatg
    15601 tttggtattg tagcgactgc tgtagttatc taagtcttga ttatattaga agctgtgaaa
    15661 acggagaaaa tattctaaaa gatatggcaa acattgtaaa tgttacagta gaatctttgg
    15721 aaactataaa cactaattca ggaggcgcac agtgggttat taaccgtcct aaagcgaatt
    15781 attggaaaaa ggtttatctg gattctaatc ggctatatcg ctaccttaga gggcaaaaaa
    15841 caaatataca aatctggaca gccgagatgt gggcacagct ttggaacatg atgtatttca
    15901 atattggtcc taaagttcac gaggaattag acttttgttt tgctactgat ccaatagaaa
    15961 aagttaaaga agtaaaaatc ttacacaatg ctggagtaac aacaaacgat gaagatttat
    16021 ttttcaaagg gagatacgtg acttctacgc cttttgatga agatttatca tttgtaaaca
    16081 agaaaaaatg ctcttacgca tatgttaaag caattaaggc ggtggttaga tgacgcctga
    16141 acaagtgact gaagaattgc taatagctgt gaaggataat atttacgtta cctggaacga
    16201 agaagatgag tcaattaaaa agatgatagc taaaaatgct gtttatcttc aaagtaaagt
    16261 gagtacaaca ctttcttttt ctcctgaaag cttagaatac ggattgctaa tcgaaagatg
    16321 tagatacgac tggaatcgtg ctttagatga gtttgaacaa aatttcgcta gtgagttatt
    16381 aggtttcatt caacattatg cgctacaaga atatattgca ggtgatgtga atggcgaata
    16441 atcgtagact cgaagaaaca ttcaacgatg gttggttaaa gattttgacg caaactacca
    16501 aaagaaatga actaggaaaa aagattggtg tagaagatac agaaatcact tctttaaaat
    16561 ttagaaatct ttccatgaga gatagtgata taacagctat ggatgcgatg ggatcgaaat
    16621 taactaagaa agtaaagact ccatttcatc caatcgccaa gaaatttaat aaagatcaat
    16681 attttatcgt aatcgatagt atgcgttaca acgttatcta tgccgattac gataattttt
    16741 atatctattt ttatcttgaa agtgtgggtg aatatggtga ttgataattc taaagaaaaa
    16801 gaacgtttaa ataagcaaat ttctgctatc aaaacttcct tagaagagca ttttggcctc
    16861 aaactctttc aagactccgt aggcgaggat gagctacctg atgattttaa ttacttcatt
    16921 ctcgaaacag gagaaataga aatgatcact gagccaaaat atagcgtggg tcaaaatcta
    16981 tatctaactt tctattcaga aaatagagaa gatttaacag gagactcact agatattatt
    17041 tcattgattc aaaatcgttc gattcgtttt cagagaatgg atcccaatca tttaaaacta
    17101 gagaaccaag atcgctatat cgatcaattg gtatttacgt ttagacgatt attgaagagt
    17161 gattgtcatg gctaaaaata gttgggagct aaaaataaat ggacatgatg aacttcttgt
    17221 gcggatggaa cgctattcaa gcgagagcga acgactgatt aacgaagcat tgaaatcaaa
    17281 aggttcggct attgcagtgg ataggattac agaaaaaatt cctgtttctg aagcagattt
    17341 aagaagaggg caccaacacg caaaaaatag tcgtccactt aagactcaat acattaattt
    17401 gggtttcatc attagaccta caagaaaatt tgagtattta aaatatcctg atttggggat
    17461 aggtacttct aaaagaaatc agccggacga atttatgaga agaggattag gtcttgcact
    17521 tgatccaatt acagaacttc tgattcgtca attcgataaa ttaaataaat agggggaaca
    17581 acaatggcta aaacaaaaac tgtagtaaca acgttcgata acataagtat caaacgaatt
    17641 gcttttaatt ttaagaacgc aggaaatgca atcgcaacga attgtaacgg acaattagat
    17701 ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag cggcagaagt aaaatcaaaa
    17761 tctaaaccaa tcaatatgac ggtaacaatt actgcacatg taccgatgga agtttatcga
    17821 cgtttcaatg ggttgaaaca agatggacgt attaaaccag gcatttactc ttacggtcct
    17881 gattccgtaa gcgaagattt ctcacttgct gcagagatcg tggatgactt cgaagaaaat
    17941 agaaagttag ttggtatgtt agcatgcact tcgaatacag gattaacatt ctctattgaa
    18001 aatggtgcgg atgaagtagc tgcgttagaa ctagaagcaa aagttatgca agatgaattt
    18061 ggtaaatttt atcatgaagc aattgttgca gaacttgaag aagacttaac agatcaatgg
    18121 atgacgaatc tatctgctga tgtgattaaa aaaacttcag ttgtggcaac tacggccact
    18181 caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa gattactcaa aaattgaact
    18241 aaacgatgga acaattttga atttagaacc taaactgaat atcaagaaat tattgatgat
    18301 caatagagat tttaacacag acgagtttgc aaaaatgtcg atgggaaaag gctctatgga
    18361 tattacagtt attcaaggtg caaaagccgt atatgtagct tatcgtcaag cgaacatggt
    18421 cgattacatt tcattcgatg aatttataga taaatgggat tttgatatgg aggttgcagt
    18481 agctgtatac agtactatga tgttcaaaca agcacgtgat gcctatcaaa aagaattcga
    18541 aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc caaagctctt agttgaaacg
    18601 tgggtcgatg tctattcgat gttgaccgac gttttttcta tgccttcaga tttggtttta
    18661 agcgatatct gtttagatga cattttgcaa atggcttaca acaagagcgc ttatgaagga
    18721 tggaagaact atgcaataaa tcaatcccag aaaaactaaa ggaaaggagg taaaaaatgg
    18781 ctaaaaagag aacggaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta
    18841 cgttaaaaga aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag
    18901 ctcaattaca acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag
    18961 aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac tagtcaacgt ttagccaatg
    19021 ccaaaaaata ttatggagaa aattccaccg aagttcagaa acttgaaaga gaactgatta
    19081 atcaacaaac agctcagcaa aggttatcta atgatataga taaaacgagt aaggcattgg
    19141 ctcaatctaa aggtgaattt aaaacgtatg agtcaactat gaaagactta gataatgagc
    19201 aacaacatct aaagtcaagc gcatcattag tagaatccga atataaaaaa tggcaagcaa
    19261 ctgctggcca gtctgctaca gaatctgaaa aattagcaaa agcacaagaa tatgtcggca
    19321 aacagagtga tattgctgaa caaaaaatcg aagtgctaaa aaaacaatta gatgccacac
    19381 aaaaagaatt tggagaaaca tctaccgaag ctttagaaat gaaaactaag cttaatgatg
    19441 cggaaagaga attcgaagaa ttaagtaatg ctgctaaaaa cgttgatacc tccacagtag
    19501 atgatatcgg caaaaaactt gatatgggta atttaatgga agctactgat cacttgtcag
    19561 tgatcggaga taagcttatt gatgtaggta gtaagtctat tgaagcagct ggaaaagcac
    19621 aagctatgca ggcccaattt aaacaagtct ttggctcttt agaaggggaa gcacaggacg
    19681 ccgttgaggg aatggctgaa gaatttggaa tgttaccaaa tacgatcaag cctgttttta
    19741 cacaatatac gtcaatgttt aaaggacttg gatatgatac caaagaagct atggagttag
    19801 ctggtgatag tactcagtta gcagcagatg cagcagcttt ttacgataag tctatggatg
    19861 atgctagtga atctcttaat tcatttataa aagggaacta cgaaggtggg gagcaaatag
    19921 gtttatttgc taatgatact caaatggcag cttatgctgt taagcataat ttgataccag
    19981 cgactgaagg agcaaaaaaa gccagtgaag agtcattgtt agctgttgaa aaagcacaat
    20041 ctaagtatgc tgatgctatt aagaaacatg gtgaaggatc tttagaagca agagaggctg
    20101 ctttaaaact taaagatgcg caagataaaa taaatgaaga attaggccca caaacgcaaa
    20161 aatggtctga tttagatgag gctaccaagc aagcagttcg agttcaatat gctgaagata
    20221 tgcaaaaatt agccggtgct acaggtcagg ctagtaggga gtctgatggt ttagaaaacc
    20281 aaatgacaag agcaaagcaa gcgttagaag atttttatgc ctcattgggt gaagatatac
    20341 tgcctgtgtt tatcaaaggg ttgcaagcag gagcgaaagc tttgcaagga ttagccgaat
    20401 ggtggagtaa acttgatggg ccaatgaaaa atttcatttt agctcttgga ggaattctag
    20461 cattattaag cacattagct cctgttataa ccgcagttgt tacgatagtt ggcacatttg
    20521 gttctacagt tttgctgcca ataataggaa ttatagcggg cgttgcagct gtaatagcga
    20581 ttgtaattac agcgtttcaa aactggggcg caatcactga ctggtttagt gatttatgga
    20641 aaaaatttac cgattggtta ggtgatacct gggaaagtat aaaagaaagt gcctcatcag
    20701 tttgggatgg agtcaaagaa acctggtctg gatttgtaga ttgggttcaa gaaatttggc
    20761 aaggagtttc tgattggttt ggagagttat ggagcggatt agttgaagga gcttccaaca
    20821 tctggcaagg agtccaagag acttggcaag cattcgttga ttgggtttca aatatttgga
    20881 acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt gttcaaatac
    20941 catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg ggtatctttg
    21001 atgtagctgg acagttatta ggcgcagctt ggcaagctgt ttggacacct atttctgatt
    21061 tccttaaaaa tacttgggat actatgacac aatggataag catcgcttgg aatggaattg
    21121 taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt atatggacat
    21181 ctattagtac tacggcttca aatatttgga attcaattag tgcaacagct tctagtattt
    21241 ggaacagtat caagaataca atcactagct tggtacaagc agctgctaca gtaattcaaa
    21301 atatttggtc aactgtatct agttggttag gtggaatttg gaattcaatc agctctacag
    21361 catcaaatat ctggaatagt gtgactagta gtataagcaa tgctataaac gcagctaaaa
    21421 gtgccattca aagtgtttgg aatagtatat cttcgtggat cagcggaatt tggaacggta
    21481 tcaaaaatac tgctttgaat ctttggaatg gaattacaag cactattagc tctaaagtaa
    21541 acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct aacaggtatt gtttccgaca
    21601 tattcaataa tgttaaaagt acaattgcta acatatggga aggcatcaaa aagactgtta
    21661 gcgctccaat tgattggatt agagacaaaa tcagtggcat ttttgataat ttgaatattt
    21721 ctataccaca tattccgtta ccggaattta tcatggaagg cagctttaac ccgctaaaag
    21781 gtcaaatccc ccatttgcgt gtaaaatggc atgctaaagg aggtatcttt actaaaccaa
    21841 ctttactagg tggaatgaac ggtgtcggtg aagcaggacc tgaagcagtt ttacctttga
    21901 aaagatctgt t
//
LOCUS       DAJSVD010000042        21825 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSVD010000042 DAJSVD010000000
VERSION     DAJSVD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.042"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(19..564)
                     /locus_tag="ON498_001415"
     CDS             complement(19..564)
                     /locus_tag="ON498_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3939676.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(576..1268)
                     /locus_tag="ON498_001416"
     CDS             complement(576..1268)
                     /locus_tag="ON498_001416"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3939677.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1287..1571)
                     /locus_tag="ON498_001417"
     CDS             complement(1287..1571)
                     /locus_tag="ON498_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939678.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1584..2147)
                     /locus_tag="ON498_001418"
     CDS             complement(1584..2147)
                     /locus_tag="ON498_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3939679.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2375..3037
                     /locus_tag="ON498_001419"
     CDS             2375..3037
                     /locus_tag="ON498_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR3939680.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3161..4357
                     /locus_tag="ON498_001420"
     CDS             3161..4357
                     /locus_tag="ON498_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR3939681.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4458..5834)
                     /locus_tag="ON498_001421"
     CDS             complement(4458..5834)
                     /locus_tag="ON498_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3939682.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5871..6737)
                     /locus_tag="ON498_001422"
     CDS             complement(5871..6737)
                     /locus_tag="ON498_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR3939683.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6826..7851)
                     /locus_tag="ON498_001423"
     CDS             complement(6826..7851)
                     /locus_tag="ON498_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3939684.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7869..8543)
                     /locus_tag="ON498_001424"
     CDS             complement(7869..8543)
                     /locus_tag="ON498_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939685.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8727..9620)
                     /locus_tag="ON498_001425"
     CDS             complement(8727..9620)
                     /locus_tag="ON498_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3939686.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9694..11391)
                     /locus_tag="ON498_001426"
     CDS             complement(9694..11391)
                     /locus_tag="ON498_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3939687.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11384..12697)
                     /locus_tag="ON498_001427"
     CDS             complement(11384..12697)
                     /locus_tag="ON498_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3939688.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12697..16734)
                     /locus_tag="ON498_001428"
     CDS             complement(12697..16734)
                     /locus_tag="ON498_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3939689.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16747..17205)
                     /locus_tag="ON498_001429"
     CDS             complement(16747..17205)
                     /locus_tag="ON498_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3939690.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17503..18021)
                     /locus_tag="ON498_001430"
     CDS             complement(17503..18021)
                     /locus_tag="ON498_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3939691.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18023..18640)
                     /locus_tag="ON498_001431"
     CDS             complement(18023..18640)
                     /locus_tag="ON498_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3939692.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18637..19500)
                     /locus_tag="ON498_001432"
     CDS             complement(18637..19500)
                     /locus_tag="ON498_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939693.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19487..20398)
                     /locus_tag="ON498_001433"
     CDS             complement(19487..20398)
                     /locus_tag="ON498_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3939694.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20439..21773)
                     /locus_tag="ON498_001434"
     CDS             complement(20439..21773)
                     /locus_tag="ON498_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3939695.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6340 a         4803 c         3771 g         6911 t
ORIGIN      
        1 tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa aatgtctcaa
       61 tgcccattca tgtccaacgg gatcgaaact tgccacggaa tctttgaaaa tatggagttt
      121 gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg tacatacgcc
      181 ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat ctgttccact
      241 aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct catagtcttg
      301 gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca cattatgcgg
      361 aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat caatggcaaa
      421 aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct ttttttcaat
      481 tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat catatgtgta
      541 gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt gacaaattcg
      601 atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa gctttgtgtc
      661 gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat attgaactga
      721 cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc agggaaattc
      781 gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt aacgatcaat
      841 ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact gcgcaaatat
      901 gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta gccaaatcct
      961 gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc tacttgtaag
     1021 ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc gtattgttgg
     1081 atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg tacgacgatc
     1141 acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc ccatgataat
     1201 ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc gataataccg
     1261 attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa aaccacacat
     1321 aacattagga taacaataat aagagatttc atcaaaaaag agttccattt ggtcgtcttt
     1381 tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc tttacgataa
     1441 aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg ctgttttttt
     1501 aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt aataagcggt
     1561 cctttggcca taagccgatc cctctacttt cctttggtca atagttccca gtattgaatc
     1621 gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg cgcctcttca
     1681 agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg attctcgacc
     1741 ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc tggtgaaaga
     1801 tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc ttctaattct
     1861 ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc agctggaatc
     1921 tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag acgatcttgt
     1981 tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct cttcgaagtt
     2041 cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc gctatagatt
     2101 tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc ttctcctgct
     2161 ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa actccttatt
     2221 agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc tttagtccga
     2281 tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg aaaagaattc
     2341 aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt ggccgtggct
     2401 tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt ttgttctagg
     2461 acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc gcaggaaaaa
     2521 gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact atgaaaaaag
     2581 cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa caaaaaatga
     2641 gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa tcgatctccg
     2701 ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag aaccaaaccg
     2761 actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg atttgacaaa
     2821 taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg aaaaactaga
     2881 agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt atcaaaaatt
     2941 cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac agagcttaaa
     3001 gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat aaaagaagac
     3061 tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta gagacattca
     3121 tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata aaccgactga
     3181 aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca acccgttttc
     3241 aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc tccaagagca
     3301 acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac aagctaaaca
     3361 gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg gatcggctgc
     3421 ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag cacaagatat
     3481 tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag ccaatcctga
     3541 cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg tcaaacaatc
     3601 gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca agatttcagt
     3661 gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag aacagcttta
     3721 ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg gagaattgaa
     3781 aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag aagaaactgg
     3841 cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc gtctggataa
     3901 acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac cacaaatccg
     3961 tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta tcgctacagc
     4021 cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc aaaaagatgc
     4081 agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga aaaattcaga
     4141 aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa tcgtggatat
     4201 cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga cattgcgtat
     4261 ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac acatggaaga
     4321 agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct tgttactaaa
     4381 acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta tctttcttct
     4441 aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc atagccaaaa
     4501 taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac agtttgcaga
     4561 cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg aacgaatggc
     4621 actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag taagcctaga
     4681 ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa tacattcaat
     4741 gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc ttttttcggt
     4801 gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc cttgctgaca
     4861 gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag tagcgttaaa
     4921 attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct cattgcaaaa
     4981 gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa gatcagcgtc
     5041 acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct tggcacatct
     5101 ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc caagaaagca
     5161 aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat gaatggctgc
     5221 cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat gataccaaat
     5281 ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag cgacacgatg
     5341 taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc attttccgga
     5401 ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa aaaaccttgc
     5461 atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc cccgatcaac
     5521 aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata ggcatatggc
     5581 ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga caaaatgaca
     5641 acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg attggccgca
     5701 acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag catgatcaaa
     5761 tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat ctctttctta
     5821 cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga ttaaactgtt
     5881 ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac aataacataa
     5941 atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa agtagagata
     6001 atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt gactgttaga
     6061 agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt ccacacaaac
     6121 tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat tccgatactt
     6181 tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat ggtatataca
     6241 ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc aagcatttta
     6301 ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca
     6361 agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca ttcatggaag
     6421 aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa tggtagccct
     6481 acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac actccaagct
     6541 aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact aacaacgaaa
     6601 aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg ttgattggca
     6661 tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat agggactaat
     6721 gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa cttatcagcg
     6781 aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac gtttttcatt
     6841 ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt cagttgattc
     6901 agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa gtcctaatcc
     6961 tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat caaagatccg
     7021 tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc tgactccttg
     7081 cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt
     7141 actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa cagtatgatc
     7201 ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct gactgcgtat
     7261 cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc gcgagaaact
     7321 gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg ataattcatt
     7381 ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt
     7441 agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat ctttttggtc
     7501 ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca gctgattgat
     7561 atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat tttgcaaagg
     7621 ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca agtaaccacc
     7681 cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa ttgtgctcaa
     7741 atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa taaaaaacag
     7801 ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca tcttaaccgc
     7861 tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata tccttggata
     7921 cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct
     7981 acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa gatctttcca
     8041 ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc aatgatctct
     8101 ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt
     8161 tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat ttttgcaatc
     8221 agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt catagccatg
     8281 atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt cgatacttca
     8341 cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc tagtaataca
     8401 agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt cgttccaaag
     8461 gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct
     8521 tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc tactacaata
     8581 tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt agcaaacgtt
     8641 aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag cttaaattta
     8701 ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg tttttgtctt
     8761 ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat acctagaagg
     8821 agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga agagacttct
     8881 tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt
     8941 gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt atttgttttt
     9001 gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc atacacttca
     9061 agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt tttctcagca
     9121 ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg aaaagaggtg
     9181 ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc aaataaaggc
     9241 aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc gtaatattct
     9301 tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac ttgtcccatt
     9361 cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac tgctaattgt
     9421 tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc gatccttctt
     9481 tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac tgcagctccg
     9541 gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt ttgaggtcta
     9601 ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc agcaaccttt
     9661 ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca ttttagattt
     9721 acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga atgccaagag
     9781 cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc tttctggccc
     9841 tggattttct actcctacga tctgaatttc ttcattatag cttccataag agatgataaa
     9901 ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca tggtctcttt
     9961 catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc catctgcgac
    10021 aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg attcataaac
    10081 agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt cattccacaa
    10141 ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt caaactttct
    10201 tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa attcatcatt
    10261 tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta gttctgtgga
    10321 aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta atctacctaa
    10381 agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa acccgttgct
    10441 tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc
    10501 tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt tgtaattttt
    10561 gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag gtgctgtgat
    10621 cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt cttgttctcc
    10681 agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca tctgtttatc
    10741 tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg tgtctttcca
    10801 tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca tcataacaac
    10861 cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga aaagatagac
    10921 tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata ccgcttctcc
    10981 agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa accaactttc
    11041 tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc
    11101 cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt caaacaaatc
    11161 aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc cgctattcgg
    11221 tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg
    11281 tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc ctattcccgc
    11341 agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca tttttttctc
    11401 cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct agtaaagaaa
    11461 tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat tgacctggat
    11521 tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc tcgttataaa
    11581 cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct aattcagcca
    11641 tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc caaaattcag
    11701 ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc acacctttta
    11761 attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta acaaggccct
    11821 ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg tctgctggtt
    11881 tgatccattg atttttcaag tcattagcag gagccatatc taaataatat ttcccccctt
    11941 ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt tcagttgttc
    12001 ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca tttttaggat
    12061 agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt ggcaaaatag
    12121 cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct ggatcaatcg
    12181 attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt gctttgatac
    12241 gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc gaagtcccta
    12301 tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc gtttcagcga
    12361 tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca tgataataat
    12421 cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt gaaatgatat
    12481 ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg tattcaaatt
    12541 gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc acgacatctt
    12601 cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc ggtagtaaaa
    12661 caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct cctttttaga
    12721 ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag
    12781 gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag gatcgttccc
    12841 gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta actcttgctg
    12901 tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt ttagtgtacc
    12961 cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc gataactaat
    13021 ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca cttccttgat
    13081 ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt ttggatctgg
    13141 tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct caattgaaaa
    13201 ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat
    13261 tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt catagccttc
    13321 cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact taccttcttc
    13381 attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg cgacagattc
    13441 cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta aatcgacata
    13501 cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac gtttcgattc
    13561 ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag aatcccccac
    13621 tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta attttctaac
    13681 tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata aacgatcctc
    13741 ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct tgagttggat
    13801 agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat caggttcttt
    13861 gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg tagtttcttc
    13921 actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt tctcaccatc
    13981 cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc ctttctcttc
    14041 ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt ccagtccgtt
    14101 accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac tgccttcaat
    14161 tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca gtttgacata
    14221 caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa gcttgtgata
    14281 ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc tcgaaccaga
    14341 tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg ctgcatattt
    14401 tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc cagcgatctc
    14461 tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg gcatcttcgt
    14521 ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt cggcttcccc
    14581 actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct cgccattttg
    14641 acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg aagaagatgg
    14701 gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct gaatataggc
    14761 catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt ttacttggta
    14821 cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat aagcttggtt
    14881 ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt gtccatgacg
    14941 ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg gatctagatg
    15001 gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa aaccagtttc
    15061 atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat cctccaaggc
    15121 tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc catcttcttc
    15181 taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt gtctcccgct
    15241 aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct cttgatactt
    15301 gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt cttgcgaagt
    15361 aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag tcgctttatt
    15421 atctccttct tttacctctc cataacgata gtgtacattt acttcatctt ctggacggtt
    15481 aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat tcccagaaaa
    15541 ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt ccatcacagt
    15601 ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg gtgcataact
    15661 gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc tattttctcc
    15721 gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa gcggagttcc
    15781 tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat tgatatcatt
    15841 tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg tactattatg
    15901 gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca gatctttact
    15961 catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg tcccagcggt
    16021 taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg gactgatttc
    16081 ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg catcttcagg
    16141 atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt tgatgtacgc
    16201 cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa gctgaacggt
    16261 ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag tagcatagtc
    16321 gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca agttctctcc
    16381 gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc aatttcctaa
    16441 cacttttgta tagtcattcg ttgtaaactt cgggtagcga tgctgttcgt tttcaacttc
    16501 tccgctagga tagatatatt cataggggtc ttgcgtagct gttggcccaa gacgctccaa
    16561 aataccgttg aataactggc gttgaatgcg ctggagtgct tgaccgctgg gtacgattgg
    16621 ttcgtccagc ttttccatct cattttcttc gctacgttga atttcagcgg aatcttctgt
    16681 atcagtttca ttttcttgta attcttggtt atctattctt tcatcaacca ccacttgaag
    16741 aggtagttac ccttcgttca catcggggat cttttctgtt tcaaaaatca atgtgccggt
    16801 ttctgcttct tttgaataaa cttttccgac ataccattct tgaccatcga tcgtctcgac
    16861 ttttaggtct tctccatctt catcatagga ggatagacct gttccacgaa cgttttttac
    16921 ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt attctacctt cattggcact
    16981 ttcctttttt tcaaaagcca gatgccattc aagggtttct tctttttcaa tatacttccc
    17041 ttcaattttt gcatagtcct catctaagaa tacctcatcc tcctttgttt ctgttgactc
    17101 agcagctaca tccagggtcg tcatatgata gactgactgg aaatatggtc caaatagcaa
    17161 actgacagtt actaactgca aaagcttcaa ttttaaccct ttcacatcct tatactcctt
    17221 tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca atgcccatct aattataatt
    17281 taatttaatt ttaattttaa atcaaatgat atccttttta gttaaaaaac accaattata
    17341 caacatttat tattaaaatt aaattataaa attttataac gttctcattt aacaaaactt
    17401 aagttattaa gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa aataaagcac
    17461 aaatgcgcat ttacgcactc atgctttatt cgtacaatta attcagataa cggcagcgca
    17521 taggatgcca ataacacttc ctaaaatagc tcctcctaaa atgtcagtag gataatgtaa
    17581 aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta atattcctgc
    17641 aactggactt aattcgaaca acgcagctac tgataaaaag gctagcgctg tatgtcctga
    17701 aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca agccgacgat ttttttcata
    17761 aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa aatagatcaa
    17821 actgccgata agaatggcta gaccatattc tctagtatca ggtatcaaga ctaaaacagc
    17881 agcaaggagc aaccaaataa aaccaccatt agagatatta ctgataaatt tcaaaaaatt
    17941 gatttttcga ctatccgttt tccgttgctt ggcgaacttg ttgataatac tatcatcgat
    18001 tttagcaatc aaacgcaaca tgttattcct cctttttaag aaccttttct tgcgtacgat
    18061 ggataaaaag caggacttga aaataaaaca agaaactgaa acctaataaa gcaaaataga
    18121 ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa tagaaaagcg
    18181 cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat ccaagaagct tctttttgtt
    18241 ccagttgaag caagattttg taactaaaga gaagcgcgct taatagaagc aaaaacaacg
    18301 tagtatacat caatgtatag ccaattcccc caccccaata gcggaaaatc tgataatcga
    18361 tcaatagaag aagaaaataa acacttgcta aactatcttg ctttccataa agagagaagt
    18421 tctcacgaaa ctttttccag aatagtgaac cgagcttttt ctcttcattt tttttgtaat
    18481 gataaatcat atacagaact gttcgacttg cagttcctaa tgtgaaaaca ggcaagacgc
    18541 acaggaacca tagtagctgt aaatggataa gacgtggaat ccattcaatg atttctaaga
    18601 aataatcatt ggctttttta ccaatcgatc gattcatcat ccttttgaac ctcctgtcaa
    18661 atccatccct tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg tcgggatcaa
    18721 gacaagtgtt gctcctgcca tcagataatt ccattgtgtc gttgcttggt tctgaaaaac
    18781 ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg atatataaca gaggacctaa
    18841 gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg caggtttcgt
    18901 caatggaagc atcaaatgac tccaaatata caaatgattc gctccgtcga tttcagcagc
    18961 ttcgatcaat tcattattga tcgataagta aaattgacgc atcagaaata tattgaacgc
    19021 attgccgaag aacgatggaa cgatcagggg caaataagta cctacccagc cgatcttgga
    19081 gaataataca tattgaggga tcatcgttac aaaccccggg atcatcatcg tagaaagaac
    19141 caaagcaaac atcagctttt tcccaggaaa atcaagcttc gcaaatccat aagcaataaa
    19201 agaatttgaa aggacattac cgatcactac tagcacagtg atgaataatg tatttcttgc
    19261 atagcgccaa aaaggaaagg cggcaatcgt atccagatag ttttcccata cgattttttc
    19321 tgggaaaaag gtcggaggat aacgaacgat ttcttccatt ggtttcaatg aagtaaacac
    19381 catccaaagc aatggaatga tcaaaatgat tcctattact gtcatgagga cataacttag
    19441 tatttttttg tttgccgatt cacactggtc tttttgaaat aatccatcat ttgccgtcac
    19501 ctccagaata atagacccac ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca
    19561 cgatcagaaa caggatcatt gacatagcca tcgcataacc catatcaaaa gctacgaacg
    19621 ccttgttcca catatgcaga ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat
    19681 ttttcgtcat gacataagct tcttgaaaaa tttggaaaga accgatcgta gaggtgatca
    19741 catcgtaaaa aataatagga gtgattaatg gcaaagtaat acttttgaac tttccccaaa
    19801 cgcctgctcc atcgatttct gctgattcat ataaagatct aggaacattt tgtaatgtag
    19861 ctaagtaaag tagcattgct cctccagaac tccatatttt catcaaaatc aacgcaggtt
    19921 tcgtccattg tggatcaaac agccaagctg gtccagggat atggaaccag cccagaaatg
    19981 tgttgatcaa tcctgcagat ggagaaagca gctgcatcca taagatgtag actgctactc
    20041 ctgataagac tgccggtaaa tagaaagctg tacggaatac actgattcct ttgatttttt
    20101 gattgagtac tgtggctaaa aacacactga aaatcgtcgt cagcggtaca ttgaagatga
    20161 cgaaataaac ggtgttgtat aacgatttcc aaaataaatc atcgtgaacg aacattcttt
    20221 tgaaatttgc tagaccaatg aaattcatct tggatgtcaa attataatcg gtgaaactac
    20281 caataactga aaaaagcata ggccctagca taaataccaa gaacccgata ataaaaggcg
    20341 agataaataa ataggcccat cttgcttctt ttttctttcg gatagataaa cgcttcaaat
    20401 tttcttgcct cctctgtacc tagacagtca aaaatggatt attgattttg cttgactaag
    20461 ttttctactg ctttttgtgc atctgccaat ccttcttttg gtgattttgt tcctaaaaga
    20521 atctggtcaa tctgttcatt cactaatgtc gtgtaatccg gagcatctaa aggaactggt
    20581 gtgatcaccg tatttttaaa gttttcgtcc gccatttgat agatcatttt tcctttatcc
    20641 gttaggttgt cattattcac taaattctca ttggctgtct tgctcgccat gatatcaaaa
    20701 cttttttcgc cgaatttttc ttgtacttct ggagaagaaa gatatttgat gaattcataa
    20761 gaggcctcag gatcttttgc tccgtatggt acttccaaga cgaatccgcc accccacgac
    20821 caatggcctg aaccttcttc tttttctggg agctgcatca cgccaaaatc aaaatcatcc
    20881 ggtgcatttt ccatcaaact agagtaataa ttgatatttt gggcacgcat ggctactaat
    20941 ccggaaataa atggatcaga tacaccagac ccaaattcgg cttctaattt attgatcgta
    21001 tttttcccat aataatcttg ccagtcttgg atccactcca aggcttccac tttattttta
    21061 gtatcaatct tgactttttc atctttgtca aaccaactag tcccgttatc tgcgtttagc
    21121 gcccatacat cagcccctaa attccataat ggataaaaac cgatgcgtgt aaattctttt
    21181 ccttcttttt tatctagttt acgtgctgtt tcttctaatt catcccaagt tgtcggtaga
    21241 tcttgttcac tgattccagc ttctttaaat atctttttat tataaaaaat gacttgtgta
    21301 tctgtattga atgggatcgc ataaggatca ccttcatatt ctactgtatc ccaaagctgc
    21361 ggataaaaat ccttagaagt gtctttttct acatatttgg agagattagt tgcttgctgt
    21421 gcttctgctc tttgggcaac ggaatttatg tcctgtacaa taacatcggg tgcatttcca
    21481 gctgttattg cagataaaga tttagtccag atgtcacccc atggctgata cttataattt
    21541 acatggatct cgttttgtga ttgattgaaa tcctcaatga tttcttcgat agcttctttt
    21601 cgtgcaccgg atccccaaaa ggaccagaaa tcaatatttt ttcttccatc acttgttttt
    21661 tcagaagaat ttgctgcact tgaactggca tcgcttgcat tacttgatcc gcatcctcct
    21721 aataaaaaca atgctgaact tgccagcaac cctgtaaaca ataatttttt catattttac
    21781 ctccaaattt taatgtaacc gatgccggtt ttaatataac gatat
//
LOCUS       DAJSVD010000043        21794 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSVD010000043 DAJSVD010000000
VERSION     DAJSVD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.043"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            52..606
                     /locus_tag="ON498_001435"
     CDS             52..606
                     /locus_tag="ON498_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3939696.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(779..1252)
                     /locus_tag="ON498_001436"
     CDS             complement(779..1252)
                     /locus_tag="ON498_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3939697.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1264..1800
                     /locus_tag="ON498_001437"
     CDS             1264..1800
                     /locus_tag="ON498_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3939698.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1840..2211
                     /locus_tag="ON498_001438"
     CDS             1840..2211
                     /locus_tag="ON498_001438"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3939699.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2495..3766
                     /locus_tag="ON498_001439"
     CDS             2495..3766
                     /locus_tag="ON498_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3939700.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4011..5036
                     /locus_tag="ON498_001440"
     CDS             4011..5036
                     /locus_tag="ON498_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3939701.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5330..6439
                     /locus_tag="ON498_001441"
     CDS             5330..6439
                     /locus_tag="ON498_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3939702.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6522..7418
                     /locus_tag="ON498_001442"
                     /pseudo
     CDS             6522..7418
                     /locus_tag="ON498_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7434..8258
                     /locus_tag="ON498_001443"
     CDS             7434..8258
                     /locus_tag="ON498_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939703.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8290..9600
                     /locus_tag="ON498_001444"
     CDS             8290..9600
                     /locus_tag="ON498_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR3939704.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9748..11007
                     /locus_tag="ON498_001445"
     CDS             9748..11007
                     /locus_tag="ON498_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3939705.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11017..11889
                     /locus_tag="ON498_001446"
     CDS             11017..11889
                     /locus_tag="ON498_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3939706.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11901..12731
                     /locus_tag="ON498_001447"
     CDS             11901..12731
                     /locus_tag="ON498_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3939707.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12771..14954
                     /locus_tag="ON498_001448"
     CDS             12771..14954
                     /locus_tag="ON498_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3939708.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15015..16235
                     /locus_tag="ON498_001449"
     CDS             15015..16235
                     /locus_tag="ON498_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR3939709.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16232..17692
                     /gene="gtfA"
                     /locus_tag="ON498_001450"
     CDS             16232..17692
                     /gene="gtfA"
                     /locus_tag="ON498_001450"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR3939710.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17769..18875
                     /locus_tag="ON498_001451"
                     /pseudo
     CDS             17769..18875
                     /locus_tag="ON498_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19197..19406
                     /locus_tag="ON498_001452"
     CDS             19197..19406
                     /locus_tag="ON498_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3939711.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19399..19548
                     /locus_tag="ON498_001453"
     CDS             19399..19548
                     /locus_tag="ON498_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR3939712.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19702..20367
                     /locus_tag="ON498_001454"
     CDS             19702..20367
                     /locus_tag="ON498_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3939713.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20651..21736)
                     /gene="xerS"
                     /locus_tag="ON498_001455"
     CDS             complement(20651..21736)
                     /gene="xerS"
                     /locus_tag="ON498_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR3939714.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7404 a         3290 c         4199 g         6901 t
ORIGIN      
        1 aaatatatac ataatcatta actcagaata tttttaagaa gggaaacaat agtgagctat
       61 aatgtacaac cgttaaggac aaatgaagag atacaagatt ttcagttttg gctaagaaga
      121 acaagtaatc cggaaagaga tagtttttta tttttgtttg gaatcaataa tgggttacgt
      181 atgtcagata ttataggatt aaaagttgga gatatacgag gcaaaagtaa gcctattatt
      241 gttgaacata aaacaggtaa gcgaaaaccg atttttatag ataatttgag agaagaaatt
      301 ttattatata ctgaaggtaa agaagaaaat gattggttat ttccgagtcg gcaacaaggt
      361 cgacatataa caagggatcg tgtctatcag atatatgcag atatagcaga aaaattagga
      421 cgagatgata taggaacaca tacattaaga aaaacttttg ggtatcacta ctacaagaaa
      481 actagagaca ttgctacctt aatgtttatt tttaatcata gctcgcaagc aattacaaaa
      541 aggtacattg gtataactga agatgaaata ggagcatcac ttcgtggatt taaactaggt
      601 gtataattta aaaattttta taaacagtaa agacaaagat taaaagctat ttttatctag
      661 aacgatttta tttcataaag ttcgcggaat aaaagctggg gttactggta cttggtatag
      721 gttcgtgtta tataaactaa gtaattaaat aacattttta ggttggaaaa ggatgaaatt
      781 aaaaaaagag aatcaatacg gtctcaaaaa cgcaaacgct gcggaataag aaaaatactg
      841 ataactcaac gtttagcaga ctatctaata gggatagtct gcttttttcg tttttgacca
      901 tacttttgac cttctgcaaa cttccttgct tttcctctgt agaactcatg aaatctgcaa
      961 aacgaattgc cgtttcctct gtctgctttt tcgcgatatg ggtgtatata tcatccatag
     1021 tggttttaat atctgtatgt ccaagtcgaa catacacttc tttgacagac gcaccagatt
     1081 caaagagcag actacaatgg gtatgacgaa aaccatgaag tgtgatatgt tttaagttat
     1141 tctctttaat gatgattttc aaattgtgat ttacatagtc taaataaagg ggcttatttt
     1201 cttctgatga aaacaaaagt tgttcaccat cttttatagg tttgattctc atttttagat
     1261 attatgcccg cacgtattca tcacatttct attgtgaatc gctttattcg accgacattt
     1321 gacttttatc ataatatact cggtttaaaa ttactaatga aaacaattaa tcaagacgat
     1381 cataccatgt atcacctgtt tttttcagat aatcaccacc gtgtgggaac tgaactgact
     1441 ttttttgaag tacaagaagg gaacaaccag ttttttggaa ccaatacgat tgaacgaacg
     1501 atactaaaag ttccttccga agcatcattg catttttggg aaatatattt tgaaacacaa
     1561 ggcgtttgcc attatggaat cgaatcattt tctggacgac ctatcttacg ttttgaaggg
     1621 ccagatgcaa cacgattagc tttagttccc ttacgcgaat ttgaagatat tgatgattat
     1681 ttcccgtatg tcacagacca gatccctaca gagcatgcca ttttaggaat tgatgccatt
     1741 caactacgcg tacaatattc cgatgcgact gaacgagagt tattgtcggt aggctggtag
     1801 cacaaagaat acgtcagttt cttttcgggt catcaacgag tgacaatctt agaaaatcaa
     1861 gacactcagt tctatcaaga agttcagatt attcaagatc gtgagaatcc agttgccatt
     1921 gaagggattg gtagcgtcca tcatgttgcg tttggcgtag aaaataaatc tgatttacaa
     1981 cgcatcgaca aacaattaca agagcgcaat tttataaatt caggaataaa agatcgtgaa
     2041 ttttttgtat cactttatta tcgtgaccca aatcaattat tgattgaaat tgctacaggg
     2101 gaaggaaacc tagatgcaaa agcgtatgaa aatcaaagtc cacgctttga agaaattcct
     2161 ttatttttac cgcagtactt aaatttcatt cgtgaacaag tggagcaata attagctaat
     2221 caaatccatg acgaagagta agaggttgac tcataagtca aaaaataaag aaaaacgcat
     2281 caaatccatt ctaaagaatg ttgatttgat gcggttccta tttttataaa ctatcatttt
     2341 cgactaataa aactacttat gagtcagcct ctcttttttt cttgattttt atacctcttt
     2401 ctaggtaaac taaacatctg ccaaaaatac cgtgtctcat tgaaacattc tcaactattt
     2461 caaaataaag gggagtacca aatgaacgag tatattgaaa ttatccacgc aagagaaaca
     2521 acttaaaaat gtcagtctca aaattcccaa aaataaaatt agtattttta ccagcgtatc
     2581 tggttcagga aaatcatcga ttgtttttga tactattacc caagaagctg gtcgtcaact
     2641 gaatgacacc tatgatagtt ttacccgttt atttttacct aaatacagtc gtccagaggt
     2701 tgatgagatt gataatcttt ctacggcgat tgtggtcaat caaaagaaga ttggtggcag
     2761 tgcgcgctca acgttaggga cgattactaa catcaatgcg ttatttcgtg tgttgttttc
     2821 atgttttgca acacctagtt taggatatgc aaatgcttat tcatttaatg atcctagtgg
     2881 aatgtgttct gtctgtcagg gaatcggtaa gacgattgca tttttaatag gattgcttta
     2941 tttttcggct attgataatg ctgattttgt attttctgat ccatttaccg cagaagtttt
     3001 gtctggaatt gaggaacagg caaagaatca tggttatttt gtattggtgc ataatgttaa
     3061 ttcagcagaa gatgttaaaa ctattcagag aaattggcgt tttgaggggt tcattgttgt
     3121 tggtgtacgt gtaaaagaat ttttggaatt aaataacgtt tttgatggtc ctgttgttta
     3181 tattgatacg catctttcaa aagagacatt aagagaactt gaacaacaaa tgaatcgttt
     3241 atttgtaaat acaaatgatt accaagctag tacattggca gtggaacatt tgattcatta
     3301 tggtcatgag aaaatagcgt ttctttcttt tgactacatt gtaggtgaac caagtgttat
     3361 tcaagaacga catcaagctt atatcgatac attaaagaaa tatcatttaa cctttaaatc
     3421 gcattggata tataacaact ctgaatttga gcgaatttat caagaattag ctgaatttac
     3481 tgcaattgtt gttactggag attatttagc ggctaaatta gtaaaatttt taaaaaataa
     3541 gaataaacga gtgatggaac aattatcgat tattggattt gatgatatta cttttgcaga
     3601 attaatggat cctgcgttga caacaatacg actaaatcca ggtaacaagg gggcgattgc
     3661 ttttcaccaa ctgattggac tggccaaaca agaagaaata aaaaatcaag tactactatt
     3721 agagggagaa ttgatttctc gagactctgt tcacaaaatc aaataaaaaa gctctgtgaa
     3781 ataagtatag ttaacaataa aatatccgaa cgatagagag aatgaactgt gatctagcag
     3841 ttcctcgtcg caaattcact agaattagca ttatttactc taaattgttc ggatattttt
     3901 tcttgttttt aattatgatt acagtgccta tttttaacga aatttgctta ttttcaaaat
     3961 gtatgattat atgttagtaa acactttcaa atatgaaaag aggagctatt atggtaggaa
     4021 ttagggatgt tgcaaaacgg gcgggtgtgt ccattgcaac agtatcgcgc gtgctaaatg
     4081 aggatccgac actttcagta accgatgaaa ccaaacgaaa gattgcagaa tcggttaatt
     4141 tttatcatta tgtcaaaaaa agtcccgttg caaaaaattt aacaaatatt gcattaataa
     4201 cgacagtttc tgaagtagat gagttggaag atccctattt tcgaacgatt cgcagaggga
     4261 ttcaagtcga agcagaacaa agcaaagtca atttgaagaa aattattcgt ttgtcagaaa
     4321 caccgttgga gcaagacgat ttgctaaaat gtcaaggtgt gttgattatt gggcaggtgc
     4381 tatcttctgt gattgaagaa gtatcagcaa ttaattcgaa tgtcgtagtg attgatgacc
     4441 ctagtattga agctgaagta gatgcagttt atactgattt aacaaaagca acgaatcagc
     4501 atttagatcg attgtataaa aaaggacacc gaaacattgc gtttatcgga ggaggacgtg
     4561 ttcgtttaga tagccaagga aataaacacg tgtcagatga tgattatcga aaaatagcct
     4621 atgagacgtg gatgaaacaa aaaggattag aaaaatatag tcaaatttat ttagaaggct
     4681 ggaccacact tgacggaatg caggcagcag atttactttt gaaaaaacat gctaaagata
     4741 agttgccgac tgctatcatg gtaggaaatg atcctattgc tgtgggggtt tatcgttcac
     4801 ttcaaaaaaa tggtctacat attccagaag atatttcgat tgtgagtttt gataatattg
     4861 aagttgctga atttttaaca cctgcattaa atacggtgaa tgtgaataca gaagaaattg
     4921 gaaaactagc agttcgcatg gttaatgaac gaataaaaga agttagaaat gtttcgatca
     4981 aagtgattgt ttctaataat attattgtta gagaaagtga aaaaaatatc gattaaaaaa
     5041 gtaaacatcc aaaaaagggt gtttactttt ttgtttacta atatttttag taaaaaatct
     5101 cgacaaaaca ttaagtaaac agttatggtg ttgttaacaa aaagaaaacg cattcaaaac
     5161 aggaggaatt aagatgaaac atttcaaaaa aatagcttta acagggatag ctgttttaac
     5221 tgggacatta ctcgcagcat gtggtggggg agatagtaat tctaaagacg ggcaagtaac
     5281 tttaagctat gcgagttgag atactgacca agctccagga ctaagaaaaa tgttagatga
     5341 atttgaaaaa caaaatcctg atatcaaagt agaaatggag acaacgcctt gggatcaata
     5401 ttgggttaaa ttagaagctg caacaacggg aggtaatatg ccagatgtag taaccatgca
     5461 ctctagcgaa tcctataaat atatgtcaca gaatgctttg atgggacttg atgatgttat
     5521 caaagaaaat gatattgaca tgggaaactt tactgaagga attgctgatt tctatacgct
     5581 tgaagacgaa ctatacgcaa tccctaaaga tgcttctgta gtggggctat ggtacaacaa
     5641 agaattattt gataatgcag gtgtcgatta tccagatgaa acatggacat gggaaacact
     5701 cagacaagct gcaattgatt taacagatga atcaaaaggc atctatggtt ttgcatcaca
     5761 tattggaaca gagactggct actggccatt tatttatcaa aatgggggag aagtatttgc
     5821 cgatgacaat acaaaatcag gtttgaatac cccagaagca caagaagctt tagcttttta
     5881 tcgagatatt gcacttgttg atcatgcttc accaacaatt acagaattac aagatcctga
     5941 taagttaagt cgttttcaat caggtagatt agcaatgatg attgaaggaa actggtttgc
     6001 accgggattt ttgaaaaatg actatctagt tgaaaatgtt gcttctgccg tgttacctaa
     6061 gggaaaagtt aatgcaacaa ttacaaatgg acttggttgg gctgccagtg cgagtacaaa
     6121 atacccagaa gaagtgaaaa aactaatggc atttttagct agtgaggacg ccaataaaat
     6181 ccaagcggaa acgggggcat caatcccagc actaaaaggc tttgaagacg agtgggctgc
     6241 ttcttcagat acaattgatt tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg
     6301 tccatacaat aaagatggat tacgagctga acaagcagaa gttcaaatta ttaaccgtat
     6361 tctgtctggt gaagtaactg ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgc
     6421 gatattgagc ggaaattaaa ttagttaatt atacaatata caggcgaaag gattctattt
     6481 cgtctgtata ttaatggatg aataccaaaa agggggatat tatgaaaaat cgaatttttg
     6541 gaaaatatcg taaagatcaa gtgatatggg gctatatttt tatttttcca acggcattag
     6601 gattaatttt attaaatata tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa
     6661 cggtagggtt cggtgatact gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag
     6721 acggtgaagt ttttcagtct ttattaaaca cattaattta tgcagtggtt tcgataccta
     6781 gtattgttgt attatccttg ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt
     6841 cgatttatcg gacaatttat tttctaccag tcgttgctgc accatccgca gtcgcaatga
     6901 tttggcgctg gatgttcaat aatgattatg gactgattaa taacatgttg gctaaaatag
     6961 gtctcgatgg accagcatgg ttaacggatc catcgattgc gatttattct attatcattg
     7021 taggagtttg gagtgctatt ggttataaca tggtgttatt actagccggc ttataagaaa
     7081 ttcctaaaga ttattatgaa gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt
     7141 tcaacattac gttaccatta gttacaccaa cattattttt tgtagttgta acaacagtta
     7201 tcaatgcttt ccaagtattt gatgttattt ttatgatgat tactcctaat agtaccgcca
     7261 tgtttaaatc acaatcatta gcctatttat tctataaaca ttcatttatt ttaaatgaca
     7321 agggatatgg ttcggcaatc gttatgttcc ttttagtgat tatattaatt attactgcaa
     7381 ttcaaatgaa gttgcagaaa aagtgggtaa actattaagg aggctatcaa attatgaaaa
     7441 ataaattttt ttcaaatgcg ccaatccata tcatgcttat tttaggtggt attgcaatgg
     7501 cattaccttt tatttggatg attttaactt cagggaaaac gttggttgaa tctacccaaa
     7561 ttccacctac aattttccct gagaaatttc agctaagtaa ctatcaacaa gtacttgatt
     7621 tattaccatt catggctttt tatatcaata catttttaat ggttgttgga cgagtgattg
     7681 gctcggtttt attttcagca atggctgcat atgcttgtgc gcgtctaagt tttccaggga
     7741 aaaatatcct tttcagctta gtacttattc aaatgatgat tcccggttcc atttttatta
     7801 ctccacagta tttattagca caaaaattgg gtattttaaa tacaattcca gcattaatat
     7861 taccaggggt agttagtgcg tttggtacgt ttttattaag acaatttttc ttaggtttac
     7921 ctgatgaatt ggaggaagcg gcctatttag aaggtgccaa tatttggacg atctttttca
     7981 aaattatgtt gccgcttgct aaatcaggat tggttgcact tggaatattc acagcgttgt
     8041 ttgcatttaa agatttaatg tggccattga ttgtcaatac ttcaattgat aagatgcctt
     8101 tagctccggg attagcaaac ttacaaggac aatatgccac gaacttccca caattaatgg
     8161 caggttcgtt attagcaatt tggccaatag ttgtgatatt catcgttttc caaagaaaat
     8221 tcattgaagg aattgctaca acaggtagta aattataaaa ctattaatta gtgattggag
     8281 agtaagtaca tgacaaaaat tacatttatt ggtgcaggaa gtactatttt tgcaaaaaac
     8341 gtattaggcg atgcgatgtt aactccaagt ctacaagatg cacatattgc attgtacgat
     8401 attgatgaaa aacgactaaa agaatcaaat ttaatgctta aaaatattaa taaaaattcg
     8461 aatgaaaatc gtgctgatat tgtttcttat tatgatcgca aagaagcgtt aagagaggct
     8521 gattttgtta tcaatgctat tcaagtgggt ggttataaac cttcaacagt gattgatttt
     8581 gatattccta aaaaatatgg gttgcgacaa actattggtg acaccatggg gattggtggg
     8641 atttttagaa ccctacggac actaccagta atgttagact ttgctaaaga aatggaagaa
     8701 gtttgtccaa atgcttggtt attgaactat acaaatccga tggcgatgtt aaccatgggt
     8761 gttttaaaag cgacgagtat taagactgtt ggtttatgtc atagcgttca agtatgcgta
     8821 ccagaattgt ttgagcattt aggaatagcc gatcaatatg acttaaatga ctttcaatgg
     8881 aaaattgcag gcattaatca tatggcattc ttgttggaaa ttacaaaaaa tggggaagat
     8941 ttctatccaa caattcgtca gttagctgca gaaaaaccaa atccacatcc agattctgta
     9001 cgttttgaat tgttgaaacg ttttggttac tatattacag aatcaagtga acacaatgca
     9061 gaataccatc catactttat taaatcaaaa tacccagaat tgattgacga attagggatt
     9121 ccgttggatg aatacttacg tcgttgtgaa caacaaatta atgattggga aagcatgcga
     9181 gaggacattg taaataatgg ggagttaaca catacgagaa gccgtgaata tgcttcatat
     9241 atcatggatt ctattacgac aggaactccg tcagtgattg caggaaatgt cttaaataaa
     9301 ggcttaatca caaacttacc gacagatgtt tgtgtggaag taccttgttt agtcgataaa
     9361 aatggcgtgc aaccaaccta tgtcggtgaa ttgccaacac aattagctgc tttaaatcgg
     9421 acgaatatca atgttcaaga actgacagta gaagccgctt taacactaga taaatcaaaa
     9481 atttatcaag cagcgtattt agatccacat ctatcatctg aattgtcttt aacagatatc
     9541 aaaaatttag tggatgattt aatcgaagca catggggact atttacctga atataattaa
     9601 aaagaagctg aaagagtaag gaattttcaa ttccttactc ttttcttagt taaatttagt
     9661 aaaaatattg acaaaacact tagtaaacag ttacattagt gttaacaaaa agaaaacgca
     9721 ttcaaattgt ataaggagga gcataaaatg aagtggtcta aaaaaattat tttagcttgt
     9781 atagcactag gagcaacgat ctctttgact gcttgtggaa aaaatcagga agatgcaagc
     9841 ggtaaggtag aaatagagtt ttttaatcaa aaaaaagaaa tgactcaaac gattcaggag
     9901 attgctaaag attttgaagc aaaaaaccct gatattcatg taaaagttgt ggatgtaccg
     9961 aatgctggtg aagtaattaa aacacgtatg cttgcaggcg atgtaccaga tgtcattaat
    10021 ctttatcctc aaagtataga attgaaagaa tgggcgaaag ctggttattt agaggactta
    10081 accgaagaac cgtatttaga gaatatcaaa aatggatatg cacaacgttt tgctattgag
    10141 gatcgtgtat atagcatacc attaaccgct aatgtctatg gtttttacta taacaaaact
    10201 gcttttgagg aaatggggat tcaagcacca gaaacttggg cagaatttga aaaaatagta
    10261 gctgagatta aagatcaaaa caaagtaccg tttgcgattg caggagcaga aggctggact
    10321 ttaaatggtt atcaccaact agctatagcg actgctgctg gtggtgaaga ggaagcgaac
    10381 aatgtttggc gtttctcaga agttaatgga attaatgctg aaagcaaaga aatgcaaaaa
    10441 gattttaatc gattagattt gttaagagaa ccaaaagctt tgcaaaacaa ctggcaaggt
    10501 gccggttata acgatacagt agttactttt acaaaaggtg atgcgttgat tatgccgaat
    10561 ggttcatggg caatgccgat gattcattca caaaatccag attttgaagt aggaactttt
    10621 ccttttccag cagatgaagc aggtcaaagt ctaacgattg gcgcaggaga cctagcctta
    10681 tcagtttctg caacttctca acataaagaa gaagccaaac gttttgtaga atatatgaca
    10741 acacctgaag cgatgcagaa atattatgat gtagatggtg caccgtgtgc tgttgagggc
    10801 gttatcgaga atactcagga ttcaccatta agtggtttaa ctgaacttgc ttttactgat
    10861 cgtcatttag tttggttagc caaagattgg aatagtgaaa atgattttta taccttaaca
    10921 acaaattatt tacacaatgg caatcaaaaa gccatgatta acgcattaaa tgctttcttt
    10981 aatccaatga aagctgacgg aaggtaggaa aataaaatga aaataaaaga aattttaaat
    11041 cgttactggg caatcatttt tgtagcaatt ccaattattt tgcagatgat ttttttctac
    11101 tttccaatgg ttcaaggtgc tttttacagt ttaactaatt ggactggtct gacttataat
    11161 tttgattttg tgggtttgaa caattataag attctttttt ctgatccgaa gtttatcaaa
    11221 tcgattggct tcactttaat tgtgacgctt tgcatgattg ctggacaaat tggttttggt
    11281 attttgattg cgcgggcgtt gaacgcaaat attaaaggga aaacattctt tagagcatgg
    11341 ttcttttttc cagcggtctt atcgggcgtg gttgtttctt tagtatttaa acaaattttt
    11401 aactatggtt taccaaaaat tggtagtatg ttaggcattg aatttttaca aaccagtctt
    11461 ttaggaacaa catggggagc gatttttgca gtaatttttg ttttactttg gcaaggaatt
    11521 gccatgccag tcatcatttt cttagcgggt tttcaaagta ttccacaaga aattattgaa
    11581 gctgctagaa tggatggggc gaatgataag caaatttttt ggaatattga aatcacctac
    11641 ttattaccaa gtatttcaat ggtatttatt atggcattaa aaggtggctt aacagcattt
    11701 gatcaaattt ttgctttaac tggtggtgga ccaagcgatg ccacaacatc tttggggtta
    11761 cttgtttata attatgcttt taaaaacaat cagtttggtt atgcgaatgc tattgcttta
    11821 gtcttattta tcttaattgg gattgtttca ctggcacaat taaaattatc gaaaaaattt
    11881 gaaatctagg aggtttccca atgaaaacat caaaagaaaa aaatatttct aaatacttgc
    11941 ttttattatt agggggcttc atgattttag ttcccttagt ttcaacgcta ttcggttcat
    12001 ttaaaacaac acccgatatt atgaataatt tctttcgttt tcctaatcca gcgactttgg
    12061 aaaatttccg aagattgtta gcggatggaa ttggtcatta cttttggaat tcggcaatca
    12121 tcacaatcat tgcagtagtt gctgtgacag caattattcc tatggctgct tattcactag
    12181 caagaaaaat gactcgtggg aaagcatacg gattgatgta tttattttta attttaggaa
    12241 tttttgttcc tttccaagta attatgattc cgattaccgt tatgatgagt aaaattggtt
    12301 tagcaaatat gtggggatta atcttactct atctcactta tgccgtacca cagacactat
    12361 ttttatatgt tggttttatt aaactaaatg tgcctatcag tctggatgag gctgctgaaa
    12421 ttgatggggc tagtcacttt acaacttatt tcaaaattgt tttcccaatg ttaaaaccaa
    12481 tgcatgcgac gactattatt cttaatgctc tatggttttg gaatgatttc atgctaccat
    12541 tattaatttt aaatcgtgat tctaatatgt ggacactacc attgtttcaa tataattata
    12601 cgggacaata ttttaacgac tatggtccaa gttttgcttc ctatacagta ggaattgtca
    12661 cgattacact agtttatctt gctttccaaa gaagtattat agctggaatg agcaatggag
    12721 cagtgaaata accaataaat tattctcatt ataaaggagt tcataaactt atgattatat
    12781 ttgacaatca cacacaaaca tttcatttac aaaataaaaa aattagctat ctattaggaa
    12841 tcgaagaaaa taattattta acgcatcttt attttggaaa aaagattacg cactattctg
    12901 gtgggtatcg ctacccccga acagatcgtt ccttctcacc caacaatgca aatgcgcaag
    12961 atcgtttatt atctttagac acattaccat tagaatttcc aggatatgga catggtgatt
    13021 ttagagaacc ggcgcataat attaaattag gaaacggtag tcgtgtaaat gattttcgtt
    13081 atgatagcta cgaaattcaa aaaggaaagc ccagcttagc gggattgcca gccttatata
    13141 cggaagatga tgatgaagct gaaacgctga ttattcaatt gaaagacgcg attagtggat
    13201 tgactttaca gttgttctac acaatttatc aaaatcacag tgtaattatt cgttctacgc
    13261 aattagtaaa taatagtgat gaaacagtgg agattaaccg cttagctagt caatcaattg
    13321 attttccaaa tcgtccactc gagttaattc atttaaatgg tacctgggca agagaacgtc
    13381 aaatgactag agaaaaaatt catacaggga taaaagtatt agatagtaaa cgcggatcaa
    13441 gcagccatca acaaaatccg tttgttgctt tagtagatgc caaaacagat gaatttcaag
    13501 gagaggcata tggatttaat ttagtttata gcggtaatca cgaaacggtg attcaaaaag
    13561 atccatttga ccaaacacgg gtcattatgg gaattaattc atttaatttt ggttggcaac
    13621 taaaaactgg cgactcattt caaacgccag aagtcgtgat ggtttatagt aatgaaggtc
    13681 tgaatacgtt atcacagacg tatcatgaat tgtataatca ccgtttaatt cgtggaaact
    13741 atcgtttaaa agaacgtccc acgttaatca acaactggga agcaacgtat tttgatttta
    13801 acgaagaaaa aatcatgaat attgtcgatg atgcgaagaa cttgggcatt gaaatgtttg
    13861 ttttagatga tggttggttt ggcggtcgtg aaaatgactt cacttcatta ggtgattggg
    13921 ttgaaacaga aggcaaactt gcttgtggtt tggaaaattt agccaagcaa gtgcatgata
    13981 acgggatgaa atttgggatt tggtttgaac cggaaatgat ttctgaagat agtgaactat
    14041 ttcgagcaca tccagattgg gcattgggta ttccaggtcg tgaccgctca attagtcgaa
    14101 atcaatatgt tcttgatttt tcaagacaag atgttcgtga taatatctat caacaaatga
    14161 cggcgatttt tgatcgtgta ccgattgatt atgtgaagtg ggatatgaat cgaaatatga
    14221 cagaagtcta ttctgtcttg ttggactcaa cgatgcaagg tgaagtggct catcgttata
    14281 tgttagggtt atatgaattt ttagaaaagt taacaacgaa ttatccaact attttatttg
    14341 aaagttgttc gggtggaggc ggtcgttttg atgcaggttt cttgtattac atgccacaaa
    14401 cttggacgag cgacaatacc gatgccgtgg ctcgtctgaa aattcaatat ggaaccagct
    14461 tagtgtatcc aatttcaagt atgggtgcgc atgtttcagc agtcccaaat catcaaacag
    14521 gaagagaaac gagtttaact attcgtggca atgctgcgat ggctggtgtt tttggttatg
    14581 agctagatct aacgcaatta acttctgaag aaaaagcaga aattaaagaa caagttgcct
    14641 tttataaaaa gcatcgccaa cttttgcaat atggaacttt ttatcgcttg gaaagtccgt
    14701 ttgaaggcaa tgatactgca tggatgtttg tctcaaaaga ccaaacagaa gccatggtct
    14761 tttatttccg agtattagca gaagctgcgc atccattagt cacattgaaa ttagcaggat
    14821 tagcagaaga agcaacttat caaatggaag atgtaacatt tgctggcgat gaattgatga
    14881 atctcggttt ttatattgac tcagaactac atggtgatta tgcgacacaa cgcttttatt
    14941 tgaaacagat atagtagctt gaatttacaa tataagtgca acaccaaaag agactcattc
    15001 caaacgaaga aaatatgttt tatttacacg catttactaa aaagcagcct gatttgaatt
    15061 gggaaaatga agaagtacgt gaagaaatct atgcaatgat taactattgg ttagataaag
    15121 gactaggcgg atttagaatc gatgcgatat taaacatcaa aaaaaaacta gagtatggca
    15181 tctttgaatc tgatggagaa gatgggttag ccttcattgg gccctggatt cttaatcagt
    15241 cgggcattga agtgtggtta aaagaaatga gagagcggac atttaaacca cataatagta
    15301 tgacggtagc agaagccgat gttcctaatg aacgtttaga tgaatatatt ggtgaagatg
    15361 gctattattc gatggttttt gattttagtt atacggatat tgatgtacct gaaactggtg
    15421 aatggtttaa agatagccaa tggacatgga cggatatgcg taccaatatt tttacgaatc
    15481 aattagttac gcaagataaa ggttggggcg ccttatattt agaaaaccac gatcaaccac
    15541 gttcaattaa taaatatatt ccagaagaat ggatcaatga ttatagtaaa aaaatgttgg
    15601 cgacgctttt tatgttacta agaggtaccc cttttattta tcaaggacaa gaactaggaa
    15661 tgaccaacat agaaatggca tcattagaag attttgatga tgtagctacg cattctcagt
    15721 ataaacgggc attagaatat ggattggcac cagagcaagc attgaaagct gtcaataaac
    15781 gaagtcggga taattctcgc acacctatgc aatggaatac gcaaaaaaat gccagattct
    15841 caacttctga taatgtatgg ttaaaagtaa atcctaatta tccagaactc aatgcagaag
    15901 ctcagttaac caatcaacag tcggtattca attattatca tcaattaatt aaattaagaa
    15961 aaaatagtgt atacaaagac gtgctaatct acggacaatt tctaccagtg aaagataaaa
    16021 atgaacatat tttgctttat gaacgccaat tagaagataa aatcgtatta gttatcttaa
    16081 acgttacagc aacagaacaa gaatattatg tagatgaaca gtaccaacaa gtattaatca
    16141 ataattatga agatgtttta ttagatcaaa gtcgaaaatt gcgtttacga ccgtttgaaa
    16201 gtattgtatt agctaattat ggaggacaat tatgaaaatt aaaaacgaag caatgctcat
    16261 cacctatgca gatagtttag gaaaaaacat gggcgaatta aaacaagtct tagacaaaca
    16321 tttacaaggt gttattggtg gtgtacatct gttgcctttt tttccatcga caggtgatcg
    16381 aggctttgcg ccgagtgatt atacgacagt tgatccagct cttggaacat gggaagatgt
    16441 ggaagcatta ggtgaagatt attatttgat gtttgatttt atgattaatc acatttctcg
    16501 tgaatcaaaa tttttccaag atttcaaaaa aaaccatgag cagtcaccat acaaagaaat
    16561 gtttatccgt attcatgaat ttttcccaga aaatcgacca acacaagaag atattgattt
    16621 aatctacaag cgtaaagaca aagcgccgtt tcaaacggta gaatttgcgg atggtacgac
    16681 agaagaggtt tggaatacct ttggggaaga acaaattgat ttagatgtga ccaaagaagt
    16741 tgtgaaagaa tttattcgag aaaccatcaa agatatggcg aaccatggct gttctttaat
    16801 tcgattagat gcgtttgctt atgcgattaa aaaattagat accaatgatt tcttcgtgga
    16861 accagatatt tgggaacttt tagacgaagt gcgtcaagaa gcagcgaaat atgacgtcga
    16921 attattacca gaaatccatg agcattattc cattcaaatg aaaattgcca atcatgatta
    16981 ttatatctat gacttcgcct tgccaatggt cactctgtat tcactttata gtggaaaatc
    17041 agaacgttta gccaattggt tgaaaatgag tccgatgaag cagtttacaa cattggatac
    17101 gcatgatgga attggtgttg tggacgctcg tgatttatta acagatgaag aattggatta
    17161 cacctctgag gaattgtata aagtcggggc gaatgtgaag aaggtctatt caagtgccaa
    17221 ttacaataat ttagatattt atcaaattaa cagcacctat tatagtgcat taggcgataa
    17281 tgaccgtagc tatctgttag cacgtgcgat tcaagtattt gcgccaggga ttccacaaat
    17341 ttattacgtt gggttattag caggaaaaaa tgatattgaa ctgttggaag caacgaaaga
    17401 agggcgtaat attaatcgtc attactatga tttagccgaa attgaagaag aagtgcaacg
    17461 gccagtgatt caacaattat ttgcactttt aaaatttaga aatgaatctg cggcgtttga
    17521 tttggatgga acgattgaag tagcaacgcc atcagaatca gaaatagtga ttacacgtaa
    17581 aaataaaaac ggtagtgata gtgcaacatt aacggctaac ttggatacga aagaatttat
    17641 cattgagtct aaagatataa aaattttcac tagtgttgca ttaaaatttt aacctattcc
    17701 atagaacaaa aaatccttta tactagcact tggatgactt atccaagacc aataaaaagg
    17761 actcatacat ggataagtat aaaacaaata cagcatttaa taaatggttt tctgccataa
    17821 aattaggaaa actttctgtc aactcccaaa aatgtatttt tgattacaat tcgtatcgta
    17881 aaaaactgtc ttttgaagct gtgctcaagc tctttctcta cgcagttaat gaagaaaaag
    17941 aaagtttgcg tgatttgagt acagctctta tcgaccgatc gctccaacta gaaaccgatg
    18001 tgtcatcgat tagtcatacc caattgtctc gagcatttaa tgcacttgaa cctaaagtgt
    18061 tagaagaaat ttttcaacaa ctgttagaaa aagttcaaca tcaaatcaaa ccaaccaagc
    18121 gaaatcatct gtatctgatt gattccagta cgttttcgct tagtttaaaa cgtcataagt
    18181 gggcgacttt ccgtcaaaca aaatcaggtg tcaagttaca cttaaactat tgttatatgg
    18241 acgatactac gatgtatcca actgatttta ccttaacaaa tgcccatgaa catgatctca
    18301 atcaactacc agtattggtg aatcaaccag aggcaactta cgtgtttgat cgtgggtatt
    18361 aggactttga aaagatggat cagatgcatt gggatggcta tttcttcgtc actcggatta
    18421 aaaaaaacac caaagttcac gtgatagata ctctggaaac atcgccagaa acagaaattc
    18481 tacgagacga gctggttcgt ttaggatcta aaacctatct aaccgccaat ttccgactag
    18541 tcactgttca agataagaac ggacgtgtgt ttcaatttat caccaatcga atggacgtct
    18601 cttcaaaaga aatctctgat atgtatcacg ctcgttggca aatcgaattg tttttcaagc
    18661 acatcaaaca acacatgacc attaaaacct tcttttctca aagtgaaaaa ggcgtgcaga
    18721 atcaattgat tttaacgatg atttccgctc tcctcacgtt tctcatcaaa ttggagacga
    18781 aaacggaaaa atcagtgttc caaatcaaac gattctttcg ttatttactg tttcagccct
    18841 ttgaatgttg tctagaaaaa ttaattccca catgagaaaa aagaaatttg gctcgttact
    18901 tttggttttc agttgaataa cagataaaaa attttctgga aaaagtcatc ctctgcgtca
    18961 gcacagtttt ttttgactta ttttcagagg aaatagtttt cagaatattc taaaatgttt
    19021 tatgcaacac tagtgatatt ttttagaatt agatttctaa tgtgcatttt ttgagtatta
    19081 aggagcaagt atacaaagat ttataaagct gattaaattt gaataaaaat atgcaggaac
    19141 gctactattt ttagtagtgt tcttttttat atttgaataa aaaatgtggt ggttaaatgg
    19201 tggttataac aaaaaatgaa ttaatgaaat taggttatgg gaagtatcag tctgagagaa
    19261 tcatacgaca ggcaaaagca attatggttt caaaaagcta tacctattac gaaaatagaa
    19321 gattaggact agtacctgtt gaatctgtag aagaagtttt aggtatgaag atagaggtag
    19381 aggaggattt tcagaatcat ggttaatttt aaaaaagata aaaatgggaa atatcatttt
    19441 actgctagtt tgggattcga caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg
    19501 ttaaaacagt taaagaagaa aaaaagcgta taccgaactt gtttctaatt atggaaaaga
    19561 agcagaaact tctcaaagta ctatgttgtt tgaggagttt ttttataaat tattcctacc
    19621 ttattatcaa gggaaagtag aagaaagaac atataaaaat cggttatcta caatcaaaaa
    19681 aacttttctt atttctttaa aatggagtta agagaaatac gtccaattga cattcaaaag
    19741 tggcaaaata atttattgag caaatataaa aattcttatg taagaaacat gtatgggatt
    19801 tttcagatgt cattagatcg agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa
    19861 gttattggaa atgtgaagaa aaggaaaact acagttgatt tttggacaaa agaagaattt
    19921 acaaaagtta tttcaacttt ttatattgat gatttgtacc aacattttgg ttttatttgt
    19981 atgtggttac tttttatgac cgttatgcgt ttgggagaag ccactgcatt aacttggaaa
    20041 gatattgatt ttgaaaataa aaacttgcgt gtgaataaaa cactctatta taagagagcc
    20101 aaaacctatg agtttgtaga acccgaaacg gcttctagta ttcgtacaat cgaactagac
    20161 gacaatacaa ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg
    20221 tcgtttgtac tttcttataa tggaacacct actcagaaat atacaatccg tcatattatg
    20281 agaagacatg caacgatcgt tggtgttcat ataatccgca tacacggatt aaggcatagt
    20341 catgcttctt tgcttatatc acaataaaaa gatgcacttc gaattaaaga aaggttaaaa
    20401 catgctgatg tacaaacgac tttaggaact tatggtcatt tgtatctaaa ttcattgaga
    20461 gagataacaa atgatcccac agattatatc caaactaatt tttctaaaat caaaccgaca
    20521 cctatttacg ataataaaaa ataaatgata aacaaaagta ggatcacacc aaaagaaaca
    20581 cactagaaaa actataatac aggcttttct agcgtgttgt ttcttatttc cgcctattta
    20641 tacattatac tcataaagca tttaatgcat cgatcgtagc cgcattgtct atatgggtat
    20701 ataaagttgt tgtattggta cttgaatgtc ccaattgttg ggcagttaat accaatgaat
    20761 ttgtctgttg gtagagtcta gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa
    20821 ttctaaagcc ttttgaatat tttgctacca tccgttcgat tgcaataccg gatatacgct
    20881 tggcttctcc ttggtaaatt gctagaaata aagctttttc gttttctgtt actttatatc
    20941 gttctttccg aatagctaga taattagcta aatattccat tgcgaatgct gctatattga
    21001 cagcatctct tttatttcct tttctagtta ctaccacatt attcttatct aaagataggt
    21061 catctaaatt aatatttata acctcggaga gtctcaatcc agatcctaaa atcagtgcat
    21121 tgatcgccag gtctctttct acatctcgtt gataatagat caaagctcgt ttactcaaag
    21181 tatttttata ttcattggca atataatcta gataattttg tgtatcctta tctagaaata
    21241 attttttctc aatggcattg gcacgtgcag ctaacgtagt tttatctgtg atggttgcta
    21301 cttgatccat aggattattg tctaagtatg gttttcctag aggattattg gaacttttcg
    21361 aaagatattt aaatagaacc tttaatgccg tgatatttct attgatagta gaagtagata
    21421 gacctaagtt attattatct ttgagcttag tttttgttgt ctcttttttt acattttctt
    21481 ttgtgcgacg tttcaaatga gatttgtaca agtttaggtc ataggcagtt aactgtctta
    21541 tcgaggtgat agaagtatca ggaacgatag actcactcag aagccattca taaaagagct
    21601 tataattttt tagatattct aaagtggttc gaggtgattg atctttatcc tcacgatctt
    21661 gaacaaaatc ttttatataa tcgggcatat gaagtatgat tgtatctatt ttttgatata
    21721 tttctgtatt tttcatgtca ttcatccttt caataagtag gggagaagag tatctctata
    21781 attggactat acca
//
LOCUS       DAJSVD010000044        21740 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSVD010000044 DAJSVD010000000
VERSION     DAJSVD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.044"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ON498_001456"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ON498_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR3939715.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ON498_001457"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ON498_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3939716.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ON498_001458"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ON498_001458"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3939717.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ON498_001459"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ON498_001459"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR3939718.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /locus_tag="ON498_001460"
     CDS             6088..6387
                     /locus_tag="ON498_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3939719.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ON498_001461"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ON498_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3939720.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /locus_tag="ON498_001462"
     CDS             6975..7211
                     /locus_tag="ON498_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3939721.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ON498_001463"
     CDS             7354..9327
                     /locus_tag="ON498_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR3939722.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ON498_001464"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ON498_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3939723.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ON498_001465"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ON498_001465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3939724.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="ON498_001466"
     CDS             12206..13498
                     /locus_tag="ON498_001466"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3939725.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14285..15148
                     /locus_tag="ON498_001467"
     CDS             14285..15148
                     /locus_tag="ON498_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3939726.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15277..16899)
                     /locus_tag="ON498_001468"
     CDS             complement(15277..16899)
                     /locus_tag="ON498_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3939727.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17148..17669
                     /locus_tag="ON498_001469"
     CDS             17148..17669
                     /locus_tag="ON498_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3939728.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17855..19585)
                     /gene="spxB"
                     /locus_tag="ON498_001470"
     CDS             complement(17855..19585)
                     /gene="spxB"
                     /locus_tag="ON498_001470"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR3939729.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19750..20568
                     /locus_tag="ON498_001471"
     CDS             19750..20568
                     /locus_tag="ON498_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR3939730.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20671..20868)
                     /locus_tag="ON498_001472"
     CDS             complement(20671..20868)
                     /locus_tag="ON498_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939731.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21037..21615
                     /locus_tag="ON498_001473"
     CDS             21037..21615
                     /locus_tag="ON498_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3939732.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         6963 a         3954 c         4605 g         6218 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgtaaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatc aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ccgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact
     4441 atatcgaaca tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag
     4501 aagcaagagc acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgtaca gccaaaggaa tcccaatcat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggtcaga gggatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaaaatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat aaccatcaag actaagtgtg taagctagca
     5881 agaataaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatt
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattagat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca atgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatattgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    13621 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    13681 ttttccgaac aaacggtgtt caaaagcttg catctacggc actaagccca atcagatgaa
    13741 aaatatggag tgctattttt atctgattgt tcttactgct tgatgcagag cagctttttc
    13801 acaacctctc cactaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc
    13861 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagct aaacggcagg
    13921 ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
    13981 ggcaaaatat gaggaactat tttttccgtt tttttctcta atactcaggt caaaacgctg
    14041 atttcacaac ctctgattca cggtgttcca gaagtagctt ctacggaaat aagccgaaat
    14101 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    14161 ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa taaaccggtt aaagtgtgtc
    14221 tagcgcaaga gatttgctat aatagacgat aagggtaaaa tgtaacgaga aggggttgaa
    14281 aaatgtgtat caaattgtaa cagattcttg ttgtgatctg ccataccatg tattagcaaa
    14341 agaaaaagtt gattttttat caatgaatct ccaattgaat gatcaagagt tagtagatga
    14401 cctcggaaag acattcaatt atgaccgcta catgaatcag ctgaaaaatg gtgcgatgcc
    14461 tacgacatca caaatcaatg ttggccgata tttagaattt ttccgttctt atgcggaaaa
    14521 acagattcct gttttatatg tggctttttc cagtggatta agcggctctt acagtagtgc
    14581 gcttcaagcg gttgaattgc tgaaggaaga atatgaagac cctgagatct atgtttttga
    14641 tacaaaagct gcaagtattg gggaagggct tttagtattg gaagctgtaa gattgaaaaa
    14701 agaaggtaaa agcatcgaag aaaccctaga gtggctggaa agtaattatt tgaaagtcca
    14761 ttcatgggta acagtagacg atctgaaata tctagaacga ggcggtagga tttccaaagc
    14821 aagtgctact cttggcagtc tattgagtgt aaagcctatt attacggtgg ataaagaagg
    14881 aaggctgacg aatgttgaca aagttcgagg aagaaacaag tcgatccaga caatcgtcaa
    14941 tgagacggta aaaacaatcg ttgcaccact tgatcaaatc gtttatgtgg cttacgctgg
    15001 ggatctagag agcgcggaga aggcaaaacg gcttttggaa gagcagataa agatgaaaga
    15061 tgtgaagctt tatcccttag gaccaacgat cgccagtcac acaggctatg gctgtatcgc
    15121 gattttttcg atgggaatca gtcgataaaa gcagtaactc attctgtaaa aaagcccaga
    15181 aattgattgt aacaaaaaca gcgactctta aaagatagat gagcatccta tctcttagag
    15241 gtcgctgttt ttttattttt taagctaaat aggccattat gcccataact cgttcaattg
    15301 tttttgaata tctgcatttt ccaaaaattc atcatatgtc gtatcagctc gatcgatcac
    15361 acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt tggataaatt gatggtcatg
    15421 tgacgcaaat aagattgagc cagtaaatgc catcaagccg tcattcaatg ctgtgattga
    15481 ttctaagtct aaatggttcg ttggatcatc taatacaagt acgttagctt tcgaaagcat
    15541 catttttgaa agcatgcaac ggactttctc tccaccagat aaaacattca ctggtttcaa
    15601 tacttcttca ccagaaaaca acattcttcc taagaagctt cttaagaagg tgttatcatc
    15661 ttcttcttta cttgcatatt gacgcaacca gtctaaaatc gttagatcag tatcgaattc
    15721 tttcgttgta tcctttggta aataagaatt acttgttgtc acaccccaac gtactgttcc
    15781 agtatctggc gtaatttcac ccatgataac tttgaatagc gttgttgttg ttatatcatt
    15841 ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta aaagatatat catctaaaat
    15901 tttctttcca tcaatcgtta cacttacatt ttctacctgc aataagtcgt ttccaatctc
    15961 acgttcaggt ttgaatccaa cgaatggata acgtctagaa gaaggttgga tatcgtctaa
    16021 agtaattttt tcaagcattt ttttacgtga agttgcttgt tttgattttg atgcattcgc
    16081 actaaagcgg gcaataaatt cttggagttc tttgatttgt tcttcttttt tggcatttgc
    16141 gtttgcttga agtttagccg ctagttgact agattccaac caaaaatcat aatttcctac
    16201 ataaagtttg attttaccaa aatctagatc cgccatatgt gtacatactt tgttcaaaaa
    16261 gtgacggtca tgggatacaa cgatcacagt attttcaaaa ttgatcaaaa attcttccag
    16321 ccaattgatc gattggatat ccagaccgtt tgttggttcg tctagcaaaa gaacatcagg
    16381 tttaccaaat agtgtttgtg ctaataacac ttttaccttt tgtccaccag ttaattcact
    16441 catttgctga tggtgcaatt cttcagggat gttcagacct tgcagtaaaa cagctgcttc
    16501 tggttcagct tcccatccat taagctcagc aaattctcct tctaattcag ctgctcgaat
    16561 accatcttcg tcagtgaaat cctctttcat gtaaatcgca tctttttctt tcagcacttc
    16621 atacagacgc ttgtgtccca tgatgactgt ttcaagtaca gtatagtctt catagtcgta
    16681 atggttctgt ttcaatacgg ccatccgttc atctggtccc atagtaacgg aacctgtgga
    16741 aggctgcaga tcccctgata acactttaag gaacgttgat ttacctgcac catttgcacc
    16801 aatcaagcca tagcagtttc cgggggtaaa tttaatattc acatcatcaa atagtttgcg
    16861 gtctgaaaag tgcaaactca catcatttac agtaatcaat ttttttcctc acttttatca
    16921 ttattagttt tgacaagtct tatagaatta tataggggaa aggtaaaaga ttcaaggcaa
    16981 tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa aataaaaata aaagcggagc
    17041 gaaaatagct actataggat ttattagttt gcaacagcat aatttttgac gttatttata
    17101 agagggagta ctataaaact gtaaaagaaa tgaaagaagg attgaaaatg gatgaaaaaa
    17161 gaaccatcga ctggggctca ttgattttgg gaattttatt tgtacttgtc tcattgttat
    17221 catttcaaga ccctgtcgga aatttagttg caattgtagt agtctttgcg atttttgcct
    17281 ttattaaagg gatatttgaa ctgtttgtac ggaacagatt aaaagagcta acaggatata
    17341 aaggaaagat gcccttaata atcgggatca tcgatattct agttggcgtc tttttcttat
    17401 tcaatattgg tgcaggagtc gcagcattgc cttttgtttt tgcagtatgg tttattgctg
    17461 attcagtttt agcgttattt actgctgact tggcaagagg agtaagtgaa ggatattatt
    17521 ggtttacgat catcgtcaat attttaggaa ttttattagg aattttctta ttgtttaatc
    17581 caatttcttc tgcgttgaca ttaagctttt tagttggatt ctattttatg ttatttggta
    17641 tcacacatat cgtctatgca tttagataag taaaatcaaa caaataaaaa agccggcttc
    17701 cgattacagt tttactgttg taattggaag ccggcttttt tatcatggac aaatatgcac
    17761 gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat gtgagaagct atatacacaa
    17821 attttatctt aatactcgaa gcatagcgca gttgtcacaa cctcttttca tataattcat
    17881 tcaatactgg caaatcatgg acttcatatt tttctttgaa tgcttggatt tcttcttcag
    17941 aatattttct tggatctaaa tgcagatctt ctactggcaa tgggcgcttg ttattgatct
    18001 tcacatcgat tactacagga cgatctgatt ttgcagctgc atcaaaggca aatcgaagtt
    18061 gcgagtattc tgtgactgta aagctatttg ccccgagcgc ttctccaact ttaccgaaat
    18121 ctgcatcttc taaaaataca ccaaatttct tttgttccgt atcttcttgt tctgcttcga
    18181 tgaaaccaaa agaatcattc gaaaagacaa cattaataat tggaagctga tatttgactt
    18241 gtgtaatgat atcttgcatg accatagcaa acccgccatc accacttaag gtaaatactt
    18301 gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg cacaccatta cccatagttg
    18361 cgaaccatcc agatgtcgta tgtttttgtt taccgttcat attcaataaa cgaatagaat
    18421 ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc gtcttcagca atacgattga
    18481 tttctttata gacagcctct ggacgaatcg gttcttcctc tcgatcttca aaagatttca
    18541 accaatttac ccatttttct ttattttctt gatttgctag gtaccagcta tcagcagggc
    18601 gagcatcccc taactcgaca agtctttgca aagctgtgtt tgcatcaccg aggatcgaca
    18661 aagatgtttc atggcgtcgt ccaaattttg acgcatcgat atcgatttgg ataaattcag
    18721 cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct accgacaaat aagatcagat
    18781 cggcttctgc caatgcttca tttccgggtt tagtagcgac acgtccagca aaaccaagaa
    18841 agttttcata atcatcagga ataattcctt ttgctagaac ggaagcgaca acaggaactg
    18901 aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt tcgcgttcct tgaccaacat
    18961 aaagaactgg tttttttgct ttttcaagat aaggcaaagc agctagcaaa tctttttctt
    19021 ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga gaaaggttgt tcgtcaattt
    19081 cttcaaaacc aagatcaaca gggatagtca cgatagccac gcctttatgc tcataggcgg
    19141 ctttaatggc ttcatctact acatgcggaa gactttgtgg tgtcatgact gtacgattgt
    19201 atacgctaac atctgcgaaa ataggatttt catttagttc ctggaaagag ttatagttca
    19261 tggatgtaga agcaacttgt cctaagaacg ctactagagg cacatggtcc atctgtgcat
    19321 catacaaacc attgatcaaa tgtgtcgcac caggtcctgc agagccgaag acagcgccga
    19381 ctttccccgt taattttgca tctgcagcgg ctgcgagtgc gccgacttct tcatgacgaa
    19441 cttgaatgta gttgatttcg gttttttctt gatatagcgc atccataatt gaattgaaag
    19501 atcctccggg aatgccgtaa atatggtcga taccccagct ttcaagaact tttaccattg
    19561 cgattcctgc attgattttt gacatggtgt ttcactcctt tagcaatgat gatacataaa
    19621 ttttttgtat ctagagtata gaacttttta tcgaaaatcc caactaattc gtcttttcac
    19681 aaaaatgtag tcatgttttt gggaatatgc tagagtagta catagataga ttcaagagag
    19741 gggaatggca tggttgaatg gctattgaga cttgaacctt ggcagcaagc acttgctggg
    19801 acagctttta cttattttat gacagctctt ggtgccgggt tggttttttt ctttaaagaa
    19861 atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt ctggtgtcat gattgctgca
    19921 agtttttggt ctttgttaga tccggctatt acaaaagcag aagaaaatgg agatattgct
    19981 tggctagttg taagtattgg gtttggttta ggcggtgtgt ttttatatat ggcagataaa
    20041 acactgcccc atatgcattt tggaccacaa catgaagcag aaggattgcc tacccattta
    20101 aaacggacga tcttgcttgt tttttctatt acattgcata acattcctga aggtttagcc
    20161 gtgggggtag cattcggtgc agcagcaact gcggataatc cgacagccgc tatattagcc
    20221 gctgtttcgg tggcgctagg tattggtatc caaaacttcc cagaaggagc agctgtatca
    20281 atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct ttgtttacgg acaagcttct
    20341 ggaatcgttg aaccaattgc tggagtgatt ggagcgctac tagtaacgaa agtggagctg
    20401 ctgcttcctt atgcacttgc ttttgccgca ggagcaatga tttatgtcgt ggtggaagaa
    20461 ttgatccctg aagcacagca gacattgtcc agcaaacgtc attttgcagt ttttggcgtc
    20521 atgagtggat ttatcattat gatgattttg gatgtggcat taggataaat aaaaaaaata
    20581 tatatgatat atcaaatagg gccgcgaact tatatcgcga ccctattttt ttagtaagtt
    20641 atatcataat gaagtgaatg gtatcataca ttaataatag ttttttctat gtgtatttcc
    20701 tttgatccag cgaacaagcc agatgataag tgcgattggg attagaactt tgaccgcaaa
    20761 ccataaaagc ccgcttagga cactgacaaa aagactgatg acgaagctag ctgcaattag
    20821 cactaaaaga accatgaaga tatttaagaa catatttttt tgacgcatac aaaaccctcc
    20881 tcttgttcat tctttactat agaaaaagta taacagggat tgtttaaaaa cacctccaac
    20941 tgaagactga tttttacttc atctacttaa atgatcaaac aattgctttt tctctggttt
    21001 atcatctaaa atagagcgga aaaggaggag aatatcatgc ctaaaaatcg tcttgaagca
    21061 tttacagatg ctgtaatcgc gattatcatg actattttag ttttagaact tcatgaacct
    21121 aacggagaca cgtgggaggc gctcgctgca ttaggttacc gattttttat ctatattgtc
    21181 agttttatca ttttagcgat ttattggaac aatcatcatc atttatttca agttgtacat
    21241 aagataaatg gacgggtctt atgggcgaac agtttctttg tttttgcgct ttcgcttttt
    21301 ccgtttgcaa cttcatggat cagtgagtac atcaatagcc tggctccaga aatgacttat
    21361 ggttttgtta ccttgaccgc taatattgcg tattatatct tggcaaaaga acttgtcaaa
    21421 gcaaataaag gccacgagaa agcattgaag gcgatgttga ataattacaa gaaatcgtat
    21481 ctatcgattg gtttgaatgt gttaggaatt gtcttagggt atttagtagc gccagtagcc
    21541 gtgctgatcg caaacatcgt gattctagct gcttggatca tcccaagtcg taccatcgaa
    21601 tcacaatatc ggtaaaatga tccgtgttta aaatttaaga gaaaaatata gaaaaaataa
    21661 ataaacgatc aaatatccga gtgatactag tattcaaaga gggatgcaga tggatcaaaa
    21721 aagaattcat gtaggactga
//
LOCUS       DAJSVD010000045        21251 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSVD010000045 DAJSVD010000000
VERSION     DAJSVD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.045"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(162..326)
                     /locus_tag="ON498_001474"
     CDS             complement(162..326)
                     /locus_tag="ON498_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939733.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            621..1949
                     /locus_tag="ON498_001475"
     CDS             621..1949
                     /locus_tag="ON498_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3939734.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2151..2984
                     /locus_tag="ON498_001476"
     CDS             2151..2984
                     /locus_tag="ON498_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3939735.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3000..3737
                     /locus_tag="ON498_001477"
     CDS             3000..3737
                     /locus_tag="ON498_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR3939736.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3892..4860)
                     /locus_tag="ON498_001478"
     CDS             complement(3892..4860)
                     /locus_tag="ON498_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3939737.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5092..5925
                     /locus_tag="ON498_001479"
     CDS             5092..5925
                     /locus_tag="ON498_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3939738.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5959..6798
                     /locus_tag="ON498_001480"
     CDS             5959..6798
                     /locus_tag="ON498_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3939739.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6824..7384
                     /locus_tag="ON498_001481"
     CDS             6824..7384
                     /locus_tag="ON498_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3939740.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7583..9304
                     /locus_tag="ON498_001482"
     CDS             7583..9304
                     /locus_tag="ON498_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR3939741.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9469..10611
                     /locus_tag="ON498_001483"
     CDS             9469..10611
                     /locus_tag="ON498_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3939742.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10627..11838
                     /gene="thiI"
                     /locus_tag="ON498_001484"
     CDS             10627..11838
                     /gene="thiI"
                     /locus_tag="ON498_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3939743.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12411..13058
                     /locus_tag="ON498_001485"
     CDS             12411..13058
                     /locus_tag="ON498_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3939744.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13187..13403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13451..16096
                     /locus_tag="ON498_001486"
     CDS             13451..16096
                     /locus_tag="ON498_001486"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3939745.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16406..17719
                     /locus_tag="ON498_001487"
     CDS             16406..17719
                     /locus_tag="ON498_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3939746.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17709..18362
                     /locus_tag="ON498_001488"
     CDS             17709..18362
                     /locus_tag="ON498_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3939747.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18417..19088
                     /gene="radC"
                     /locus_tag="ON498_001489"
     CDS             18417..19088
                     /gene="radC"
                     /locus_tag="ON498_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3939748.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(18983..20335)
                     /locus_tag="ON498_001490"
     CDS             complement(18983..20335)
                     /locus_tag="ON498_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3939749.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(20401..>21251)
                     /locus_tag="ON498_001491"
     CDS             complement(20401..>21251)
                     /locus_tag="ON498_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCR3939750.1"
                     /translation="IKKKMLIPLESEITGLLNKLTISNVDSTREKIIRTFDKINKLFK
                     NDEAEFKKELLELKLQELELNYSYIVKVNDEKEQQKAIKEQMIEEEKVRREIEREKKR
                     IDKEERQFNSEITKLMAYMQKASADVEKELYADKIKELEEKLRELETVKEDVLQRELN
                     TRAGYVYVISNIGSFGEDVYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAP
                     TLENQLHSHFRKNEVNKVNQRKEFFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRS
                     LELTDV"
BASE COUNT         7021 a         3583 c         4292 g         6355 t
ORIGIN      
        1 taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc
       61 acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta
      121 tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca
      181 tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga
      241 aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa
      301 aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc
      361 cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc
      421 tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat
      481 tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa
      541 tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt
      601 ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca
      661 aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa
      721 ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag
      781 atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg
      841 cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat
      901 cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca
      961 tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc
     1021 ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg
     1081 tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact
     1141 acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag
     1201 aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct
     1261 tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa
     1321 agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg
     1381 taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc
     1441 gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga
     1501 atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg
     1561 tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag
     1621 atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt
     1681 tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag
     1741 acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg
     1801 gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa
     1861 aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc
     1921 cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa
     1981 gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt
     2041 atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat
     2101 taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc
     2161 aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg
     2221 ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga
     2281 atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg
     2341 tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg
     2401 aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc
     2461 atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc
     2521 agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc
     2581 agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc
     2641 gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc
     2701 acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg
     2761 acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg
     2821 gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga
     2881 aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag
     2941 aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt
     3001 tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa
     3061 cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc
     3121 agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt
     3181 attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag
     3241 ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga
     3301 aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga
     3361 gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc
     3421 gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt
     3481 atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg
     3541 tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata
     3601 aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg
     3661 gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta
     3721 tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa
     3781 gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag
     3841 ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg
     3901 gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa
     3961 acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt
     4021 tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag
     4081 tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag
     4141 attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc
     4201 caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc
     4261 ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt
     4321 tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt
     4381 ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc
     4441 tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact
     4501 tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat
     4561 cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac
     4621 tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga
     4681 aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt
     4741 ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt
     4801 gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat
     4861 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     4921 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     4981 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     5041 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     5101 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     5161 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     5221 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc
     5281 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     5341 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     5401 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     5461 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     5521 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     5581 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     5641 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     5701 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     5761 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     5821 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     5881 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     5941 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     6001 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     6061 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     6121 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     6181 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     6241 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     6301 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     6361 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     6421 gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca
     6481 gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat
     6541 actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat
     6601 gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag
     6661 atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat
     6721 gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac
     6781 ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc
     6841 ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac
     6901 ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga
     6961 atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca
     7021 cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc
     7081 gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc
     7141 attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa
     7201 attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga
     7261 aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat
     7321 tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga
     7381 gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt
     7441 ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg
     7501 agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat
     7561 actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat
     7621 cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga
     7681 tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga
     7741 agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg
     7801 gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt
     7861 cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga
     7921 agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa
     7981 ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta
     8041 tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga
     8101 attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac
     8161 ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga
     8221 agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc
     8281 tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt
     8341 cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt
     8401 agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga
     8461 tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa
     8521 aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt
     8581 attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg
     8641 tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc
     8701 acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt
     8761 caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt
     8821 gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag
     8881 tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt
     8941 cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt
     9001 aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga
     9061 taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc
     9121 aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt
     9181 attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg
     9241 agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt
     9301 ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc
     9361 tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct
     9421 tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt
     9481 gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt
     9541 cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg
     9601 ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat
     9661 tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa
     9721 agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca
     9781 gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga
     9841 tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt
     9901 atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg
     9961 gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca
    10021 ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat
    10081 ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg
    10141 acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg
    10201 gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca
    10261 caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt
    10321 tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc
    10381 gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc
    10441 tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg
    10501 gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa
    10561 tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt
    10621 gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag
    10681 aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt
    10741 ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac
    10801 gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt
    10861 attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa
    10921 atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa
    10981 ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat
    11041 atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc
    11101 gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga
    11161 aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg
    11221 aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa
    11281 gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat
    11341 tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga
    11401 tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt
    11461 cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg
    11521 caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca
    11581 atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc
    11641 cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa
    11701 ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca
    11761 ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa
    11821 ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac
    11881 aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg
    11941 attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa
    12001 taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc
    12061 atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga
    12121 aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga
    12181 acaccgtgta tcagaggttg cgaaaaagct gccctgcatc aagtaataag aacagccaaa
    12241 caaaaatagt tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt
    12301 gaacaccgtt ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt
    12361 tagaaacatg ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa
    12421 aaacagatac actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga
    12481 agatgctgga tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc
    12541 aaattccttc tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg
    12601 gatatggcta tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag
    12661 aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca
    12721 attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga
    12781 tcaatcgagt aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca
    12841 aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta
    12901 ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa
    12961 ctgtcccagc ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta
    13021 tttactttga tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg
    13081 atcttgcgag aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt
    13141 tttcacgacc acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa
    13201 caagaggagt aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca
    13261 atgacaacca gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag
    13321 tcgtgatcgt taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac
    13381 cgcgtaaatc atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga
    13441 ggaaaacagc atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga
    13501 agctggacgt tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa
    13561 agcaaaacct tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg
    13621 gcatgcttgg gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata
    13681 tgatacttta tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga
    13741 agcgaagctt agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga
    13801 tcaagtgtgg gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat
    13861 gggcttatct ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc
    13921 agtacgtaaa gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat
    13981 catcaactgg gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga
    14041 tatcgaaggc gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga
    14101 aattgcgaca acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga
    14161 agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca
    14221 aatcccaatc attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat
    14281 cactccagca catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt
    14341 aaatgttatg gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga
    14401 acgttttgct gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa
    14461 aatcgatacg atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc
    14521 tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa
    14581 ccaagaaaca gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg
    14641 ttggatggaa aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat
    14701 tcctgcatgg tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga
    14761 tagtgaaaac tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg
    14821 gcctttctca acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc
    14881 tactagcact ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt
    14941 ccaaagcttg gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat
    15001 tcgtgacgaa caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga
    15061 agtaatcgaa aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc
    15121 aggacaagat gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa
    15181 aatctggaat gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat
    15241 cgatttatct ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac
    15301 gatcgaacga gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact
    15361 gtataacttt atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact
    15421 ttatggagat gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga
    15481 tcagatccta cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca
    15541 tattcctcat cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt
    15601 tgatgatgaa acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg
    15661 aaatattcgt tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac
    15721 aaacgatgct aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg
    15781 taatcccgaa gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc
    15841 tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga
    15901 aatcaaacga ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg
    15961 aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg
    16021 tgcaaaagaa aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt
    16081 gcgtactatt caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag
    16141 ctagctgatt gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat
    16201 aaggagatgt gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga
    16261 gaaggaggga ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc
    16321 tctctgtttc ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa
    16381 taaaagagaa atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag
    16441 tcgattacca tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt
    16501 agggcatcca gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc
    16561 tactgtagct tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac
    16621 ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga
    16681 caatgatttg atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt
    16741 tgaagcagta gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt
    16801 tttgatgaag aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc
    16861 taccaatgta gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga
    16921 aatacttgga gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg
    16981 cgttccagta gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc
    17041 taaagcaaaa aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca
    17101 tcctgatgag atgtggggag aagtatttga attttttaat gaaataggaa agatccctca
    17161 attaaccact ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt
    17221 gtattttgtt tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg
    17281 attgaagaaa tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt
    17341 tttagatggt gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga
    17401 attcaaagga tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga
    17461 aatgctagaa cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca
    17521 tccaaaagcc atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt
    17581 ttcattgtgg caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt
    17641 agttgtgacc ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg
    17701 aggagatcat gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag
    17761 agttagtgta ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata
    17821 aagacctgta tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata
    17881 tgattttctc ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg
    17941 aaacgcttga acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc
    18001 tctctttttt agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag
    18061 tcattgaact tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg
    18121 cagaaaatgt tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc
    18181 taggtacagc gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag
    18241 cttctgcggc tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat
    18301 tgatcaaaaa aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat
    18361 aaaaatccat ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg
    18421 gaaaacgatt gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt
    18481 atggcgcgga agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac
    18541 acccttatag tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct
    18601 ctttacgaca ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag
    18661 caatcgaaat caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag
    18721 cggctccacc ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag
    18781 atcacaaaca agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa
    18841 aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc
    18901 attatgctgt acgttattgt gcagcaagga tcgttttagc gcataatcat ccttccggaa
    18961 tatataagtt agtacacata aattatataa ggaaatttac tgttatattg ccatctggaa
    19021 atactattat gttatcgata attgaacgcc ataatgcatt tttttcttct tccgaagatt
    19081 ctttataaat ttcatcgaat tcttttgata gaataaattc gacatttttt atatctattt
    19141 tagctggctc tacttcaatt tcatttaatt ctttcattaa agcttctcga tcttttttgt
    19201 attcctctaa gtctataact tcattcaagt aggctttctt caatctatct aatttattct
    19261 caatattttt cttactattt ttctttttaa taatctttgg ttgttttttt gaatagttta
    19321 tttgtatttg ttcaaatttt tgtttagtag cttcaaaaat ataacgttct aaattttttt
    19381 cgtaaaaagc gcgcttattt gtgcaacgta atttattatt tcttcgtttt acacatctat
    19441 aacaagtcct ttcgtatata gattctgtac cgtctttttt cttatatctt gctttaatag
    19501 tttgaccagc tactgatccc tgacagtcag aacattttaa taaaccttta aaaatgtaat
    19561 cacgttttgc atttgttttt aaatttttag gtaaggcttt ttgaacagca taaaaaagtt
    19621 ttttatcaat aataggagga cagtaatttt tattatttct taattcacca atgtattttt
    19681 tgttcgtcaa catattttta acactttgat aatctctaac gattccatac tgagagccta
    19741 aataataaac agtttttctt aaactaccac tagaaagaaa gtagttgaat atatctatca
    19801 cgataggagc atcatcattt attactaatt tcttattttc tatagagtag cctaacggag
    19861 ttttcccgct tactacttca ccttgtgcaa ttttatttgc cataacagct ttgatgcgtt
    19921 ctgaatcaat ttgggcttcc aattcagcaa aactcattac ttgtgcaatg aaagttctgc
    19981 catacgctgt agttgtatca taatattgtt gagaaacagc attccaagaa acattgtgtt
    20041 tttctaaaat ttcttgagtg tttaaataat gtcgtagatt tctaaaccat ctatctaatt
    20101 ttgtaaataa aattagatct attttatttt cttttacatc ttgtaatagt ttttgaaatt
    20161 catcacgatt tattttttga ccagaaatac caccgtctac atattcatta actactttca
    20221 aatccttgtg ttgttcgata tattcataga gggtttcttt ttgttctcta agactatctc
    20281 catgtttggc ttgttgttca gttgatactc tcatatataa tgctactcgt ttcatgttat
    20341 ctcctttcga atgtatgttc ttatcgttgt aaaagaaaag cccgaaggct aatcttttta
    20401 ctacacatct gttaattcta atgatcttct gtattgttca gcttgagcta gtttagtaaa
    20461 agatacagtt ccgttataat tttctaaaac aactttttca atttcatcaa gagatacttt
    20521 aaagaattct tttcgttgat taactttatt gacttcgttt tttctaaaat gcgaatgtag
    20581 ttgattttct aatgttggtg catcttctga aaatatcatt gcatgaacat caaattcaaa
    20641 aggtacagaa gcgctactaa gttccttaat tctatccata ggttctaacc gtctagtcat
    20701 tcctatttta tagacatctt caccgaaaga tccgatatta ctgatgacat aaacatagcc
    20761 agctctagta ttaagttctc tttgcaatac atcttctttg acagtttcta gttctcttaa
    20821 tttctcttcc agttctttaa ttttatcagc atagagttct ttttcaacat cagcactagc
    20881 tttttgcatg tatgccatta acttagttat ctctgagtta aactgtcgtt cttctttatc
    20941 aatgcgtttc ttttcacgtt ctatttcacg tcttaccttt tcttcttcaa tcatttgttc
    21001 ttttattgct ttttgctgtt ctttttcatc gttaacttta actatgtatg aatagttgag
    21061 ttctaattct tgaagtttta attcaagtaa ttcttttttg aattctgctt catcgttttt
    21121 aaaaagcttg tttattttat caaaagttct tataattttt tctctagttg aatctacgtt
    21181 agaaatagta agtttattga gaagtccagt tatttctgat tctaatggta taagcatttt
    21241 ctttttaata t
//
LOCUS       DAJSVD010000046        20954 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSVD010000046 DAJSVD010000000
VERSION     DAJSVD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.046"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            591..1280
                     /locus_tag="ON498_001492"
     CDS             591..1280
                     /locus_tag="ON498_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939751.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ON498_001493"
     CDS             1267..2475
                     /locus_tag="ON498_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3939752.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ON498_001494"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ON498_001494"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3939753.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ON498_001495"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ON498_001495"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3939754.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ON498_001496"
     CDS             complement(6578..7273)
                     /locus_tag="ON498_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR3939755.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ON498_001497"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ON498_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3939756.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ON498_001498"
     CDS             complement(8472..8663)
                     /locus_tag="ON498_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR3939757.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ON498_001499"
     CDS             complement(8684..9574)
                     /locus_tag="ON498_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR3939758.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ON498_001500"
     CDS             complement(9561..10328)
                     /locus_tag="ON498_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3939759.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ON498_001501"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ON498_001501"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3939760.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ON498_001502"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ON498_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3939761.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ON498_001503"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ON498_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3939762.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ON498_001504"
     CDS             14899..15282
                     /locus_tag="ON498_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939763.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ON498_001505"
     CDS             complement(15450..16208)
                     /locus_tag="ON498_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR3939764.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ON498_001506"
     CDS             complement(16226..17044)
                     /locus_tag="ON498_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3939765.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ON498_001507"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ON498_001507"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3939766.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ON498_001508"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ON498_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3939767.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ON498_001509"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ON498_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3939768.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ON498_001510"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ON498_001510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3939769.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5990 a         4264 c         3838 g         6862 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttt
//
LOCUS       DAJSVD010000047        20532 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSVD010000047 DAJSVD010000000
VERSION     DAJSVD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.047"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..561)
                     /locus_tag="ON498_001511"
     CDS             complement(<1..561)
                     /locus_tag="ON498_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR3939770.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ON498_001512"
     CDS             complement(748..1398)
                     /locus_tag="ON498_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939771.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ON498_001513"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ON498_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3939772.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="ON498_001514"
     CDS             complement(2910..3908)
                     /locus_tag="ON498_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3939773.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ON498_001515"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ON498_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3939774.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ON498_001516"
     CDS             complement(4924..5655)
                     /locus_tag="ON498_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3939775.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ON498_001517"
     CDS             complement(5659..6924)
                     /locus_tag="ON498_001517"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3939776.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ON498_001518"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="ON498_001518"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3939777.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ON498_001519"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="ON498_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3939778.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="ON498_001520"
     CDS             12266..13768
                     /locus_tag="ON498_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3939779.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="ON498_001521"
     CDS             13770..14507
                     /locus_tag="ON498_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3939780.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14678..15871)
                     /locus_tag="ON498_001522"
     CDS             complement(14678..15871)
                     /locus_tag="ON498_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3939781.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15950..16156)
                     /locus_tag="ON498_001523"
     CDS             complement(15950..16156)
                     /locus_tag="ON498_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3939782.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16149..17291)
                     /locus_tag="ON498_001524"
     CDS             complement(16149..17291)
                     /locus_tag="ON498_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3939783.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17803..18438
                     /locus_tag="ON498_001525"
     CDS             17803..18438
                     /locus_tag="ON498_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3939784.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18592..18669
                     /locus_tag="ON498_001526"
                     /pseudo
     CDS             18592..18669
                     /locus_tag="ON498_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18914..19030
                     /locus_tag="ON498_001527"
     CDS             18914..19030
                     /locus_tag="ON498_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3939785.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19046..>20532
                     /locus_tag="ON498_001528"
     CDS             19046..>20532
                     /locus_tag="ON498_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3939786.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVT"
BASE COUNT         6035 a         4221 c         3639 g         6637 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattcctc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaaaat aaattatata
    14521 tgtctttttg actatctttt tatatcttct taaatgtccg caaagcctta ttttataggc
    14581 tctacgggca ttttgctttt gtggtaaacc tcacatatct aggtctatct tcttatattt
    14641 tcactatcaa gcgtggttaa aatcgtggta aatacttcta tgcaattaga cgctgaacct
    14701 ctgattttgc ggtatctatg gacgcatgag cataccagtt cattgtgata ctgatatttg
    14761 aatgccccat gatatactgt aaatcttttg ggttcatgtt cttgcttgcc agtcttgtgc
    14821 agaatgtatg gcgtagcgta tgcggtgtga tatgtggcaa tggattgtcc ttgtggtgct
    14881 tgttgtattt ctttaccata cggataaata aggcgttata atcaatcgca actttgggct
    14941 tgcccttatg attgacaaat aggaagttgc cccgtccgtc tatcacaaat ggttctgcct
    15001 ttggacgttt tttcataacc tgttgaaatg cctgtatcgt ttctttgctt aatggtactt
    15061 gccttattcc gctttttgtt ttaggcgttt caatataata gccctgttcc ttgctcttta
    15121 gtaactggtg gtcgataatc acaacttcat tcttgaaatc aatgtcggct actgtcagtc
    15181 cgcacagttc cgaaatacga agtcctgtct ttaacagtat cagcacatca tcataatgct
    15241 tgtgatacac attgtccgtc ttgatgaatg agagtaaggc ttgttcctgt tcctctgtca
    15301 atgcgacttt ctttttggta tcattttcta ggacttcact caacttgaaa tcaaagggat
    15361 ttttcctcac ataatcatct tgaatggcta tgtagaatga cgcttttaac gagcgtttat
    15421 ggttgttaat ggtattgtag gaaaagcctt tgtctttcat gcgtaacgcc cattccttag
    15481 cgtcagaggg tttgattgta tcaatgctcc tagcacctaa cttgtcctct ttcaataacc
    15541 gcatgagttg ttcccgttgt ttctgtgtgc ttttcttcac atttgccctc tgtgcgttct
    15601 gtttggcata aagttggcaa agcgtcattt tcttgccagt gctgtcgata ccgtcctcaa
    15661 tatcccgtct tatctgctgt tccagttctc gaagtgaggg tttttcccgt tttccctttg
    15721 gtgtcgggtc tgtgggtgtc aatctccaag catacacata ttttgtgttt ccaaatgcgt
    15781 ctacatattt atataagtat tttctgtctg ttcgttggct ctctccagta tgcaggatac
    15841 gacctttgtt atcccgtctt ttttctttca tggtgtctgc tcctttcctt gttggaaaga
    15901 gccttgatat gacttgtgtt catcataaca catacaaggc tcatttgcat tagatagcgt
    15961 ccaatttatc aatgacctgt tcaaactgtc ggcgtttaat ctgtatgcgg ttgccattca
    16021 taatgagcca gccagcgtcc ttgttttcct ctgccaatcg tcttaacttg ttttctccga
    16081 tacggaaata ctttgacgct tcttcaatgg taagggtgta tttttcccat acgggaatat
    16141 cctgtgcatt actcataaaa caccccctca ctttcgatta cgtctgctat gtccgtacac
    16201 atctgttcca caagcatttc ctgcttttca gagaggtggg tacattcatc aatcattttc
    16261 atgtagtttg agcctgtcag cctgtcgatt tcctctatca tcacgattga cggcatgacc
    16321 tgtctttcca gccattgcag agtgcgttcc atattgacag gtacggggtt catttcaaaa
    16381 ggtaaactgt catgggaaat gaatatctcc cacagcggat agtcgggaaa ttgcacaaaa
    16441 tcagtaataa ggtaaaacac cagcccatga gggttatatg tcaataacaa ttcctcaact
    16501 gcctgtactg cttttgtacc acgcagacga atttcaaaac gattgataat atccgtatgt
    16561 ttctttttgg ttgcctgttc cttttgtttt tcatacaggc agaagtattt tgtgcttgcc
    16621 tttgaaccga tataaagggt gcttgctaaa ttacggtttt ttccgcttag ttttccaccc
    16681 tgcacatttt cataattttt acagcgacaa tccgcaccgc catttctgta tttttcagac
    16741 aaaacgggaa tatccaacag accacacata tcattgattg ccaagtcaaa acgtcggata
    16801 acaccgtcac agtcaagaca gcggtttaaa aatgaatacc aatctctgtt ctgtgcctgc
    16861 aagacatatt ccatgttgcg tgagcctgcc ccttttaatt ccaagaatac gcccatgtgt
    16921 tcgtcatcag aagccatgac cttgatttct ccgtaggcgt aatgctcttt atagccataa
    16981 tacccatagt cataatggat aaaataatct gctttcatgc caagcacctt tcggataatt
    17041 tccaatgcgt cagtcgttgg ataacggaca gacagataat caacgagcag ataaaaaggc
    17101 ttatcgcaga tataataatt cagacaacgc aggataatat tttgtgtttc cttgctggcg
    17161 ttggcttttc cgttctcaaa gcggttgata ctatatcttg atacatgggt aagacttgca
    17221 agttctcctt gtgaaagacc gcttgcaaga cgtttgttct tcatttcaag tgcaaattct
    17281 aaattgttca ttgtattcct ccaatcccac aatagccttg aaatacctgt aaacctctat
    17341 tcaaaaagtg tgatatgcac tatcttctta aaacttctca ttactactca caactttggg
    17401 attgtatggt ttttgacttg tcttttgtga aatagtgccc ccctgttaga taacgggggc
    17461 acagacaagg ctcgcaggct cgccagccga caaagcattt cttccccttg acacaaggtg
    17521 tcatggcggt aagcctgaga ggcttccgcc accgtgtcag aaatactttg tcagcaagtt
    17581 ctgttttgcc ttttctaaca agttccattt caaccgatat tcatatctac ggatttaatc
    17641 agataggtat ttctttttga atgtttttat atttcattct attgtgctta ttcgattaca
    17701 agaacatagt aattcttata tgcttatttg tcaatatctt tttttgcctt tacaaatcat
    17761 attaagcatg atagacttat aatagcgaag atataacgag atatggaggg ttatgcaatg
    17821 aaaataagcc atttaggaaa taatatacag accataagaa aatttagggg aatgaaacaa
    17881 caggaacttg cggacaaaat cggtatcaat atgcagagcc tttccaagat tgaaagaggg
    17941 ttgaactatc ctgcctatga aacgctggaa aagataatgg aagtgctgga cgttacgccc
    18001 aatgaattgt tgtcgggaga atggaagtat gtcaatcaat ctgaaaagga agtctgtcag
    18061 tttttaagag ccgaagaacg tctaaatgca gaactgaaac atggacacta tgataacttc
    18121 tttgacagcg aggaagaatg gctggaatat gagttagaaa agttacggga atatattaca
    18181 gactatatca acgggaaaag cattgaagcg tccgaccttt acccaatcaa agaatttatc
    18241 cagcatttga agtttcaaaa attattagac cgctatgatg atttatacag tatggatatg
    18301 tttggggaaa gcatagaggg acataagtat aagacacctt atcaagtcgt gaaaatgata
    18361 aacccgaact caaaagaaga tatggaactg ttacgagaag tattaaggaa taacaatttt
    18421 gatgatgagg acgagtaact tttttcctct tgcgagaaga tacagcaaac aaaaagcccg
    18481 atcaagagtg caaagcacca ccaatggtgg caaagctctt gacagggctt tttcttttct
    18541 gttgtgggca atcaagagga agaaagggat aaaatctgat ttgtggtaaa tcaggcaaag
    18601 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
    18661 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
    18721 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
    18781 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
    18841 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
    18901 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
    18961 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
    19021 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
    19081 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
    19141 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
    19201 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
    19261 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
    19321 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
    19381 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
    19441 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
    19501 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
    19561 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
    19621 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc
    19681 tctgttccct ctttatcatg gaagtgcaaa aagtaatata gggattgata accttataga
    19741 agttattact aataaatttt attcatcaac acatcgaggt ccgtctgaac tttgcggaaa
    19801 tgttttcaaa attgaatata caaaaaaaag acaacgtctt gcatatatac gcctttatag
    19861 tggagtacta catttacgag attcggttag agtatcagaa aaagaaaaaa taaaagttac
    19921 agaaatgtat acttcaataa atggtgaatt atgtaagatt gatagagctt attctggaga
    19981 aattgttatt ttgcaaaatg agtttttgaa gttaaatagt gttcttggag atacaaaact
    20041 attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa ctgttgaacc
    20101 gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct cagatagtga
    20161 tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt ctttcttagg
    20221 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
    20281 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
    20341 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
    20401 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
    20461 gtttcaaaat gcagttatgg aggggatacg ctatggctgt gaacaaggat tgtatggttg
    20521 gaatgtgacg ga
//
LOCUS       DAJSVD010000048        18905 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSVD010000048 DAJSVD010000000
VERSION     DAJSVD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.048"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(107..1405)
                     /gene="eno"
                     /locus_tag="ON498_001529"
     CDS             complement(107..1405)
                     /gene="eno"
                     /locus_tag="ON498_001529"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3939787.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1521..2276)
                     /locus_tag="ON498_001530"
     CDS             complement(1521..2276)
                     /locus_tag="ON498_001530"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3939788.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2380..3570)
                     /locus_tag="ON498_001531"
     CDS             complement(2380..3570)
                     /locus_tag="ON498_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3939789.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3680..4681)
                     /gene="gap"
                     /locus_tag="ON498_001532"
     CDS             complement(3680..4681)
                     /gene="gap"
                     /locus_tag="ON498_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3939790.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4722..5813)
                     /locus_tag="ON498_001533"
     CDS             complement(4722..5813)
                     /locus_tag="ON498_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR3939791.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="ON498_001534"
     CDS             complement(5962..7296)
                     /gene="rpoN"
                     /locus_tag="ON498_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3939792.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7356..7427
                     /locus_tag="ON498_001535"
     tRNA            7356..7427
                     /locus_tag="ON498_001535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7389..7391,aa:Arg,seq:ccg)
     gene            complement(7619..8503)
                     /locus_tag="ON498_001536"
     CDS             complement(7619..8503)
                     /locus_tag="ON498_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3939793.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8590..9489)
                     /locus_tag="ON498_001537"
     CDS             complement(8590..9489)
                     /locus_tag="ON498_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR3939794.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTQSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9506..10012)
                     /locus_tag="ON498_001538"
     CDS             complement(9506..10012)
                     /locus_tag="ON498_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR3939795.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10201..10821)
                     /locus_tag="ON498_001539"
     CDS             complement(10201..10821)
                     /locus_tag="ON498_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR3939796.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10897..11334)
                     /locus_tag="ON498_001540"
     CDS             complement(10897..11334)
                     /locus_tag="ON498_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR3939797.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11497..12327
                     /locus_tag="ON498_001541"
     CDS             11497..12327
                     /locus_tag="ON498_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR3939798.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12311..13678
                     /locus_tag="ON498_001542"
     CDS             12311..13678
                     /locus_tag="ON498_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3939799.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13697..14506
                     /gene="yidA"
                     /locus_tag="ON498_001543"
     CDS             13697..14506
                     /gene="yidA"
                     /locus_tag="ON498_001543"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3939800.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14549..14992)
                     /locus_tag="ON498_001544"
     CDS             complement(14549..14992)
                     /locus_tag="ON498_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR3939801.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15126..15497
                     /locus_tag="ON498_001545"
     CDS             15126..15497
                     /locus_tag="ON498_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3939802.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15666..16448)
                     /locus_tag="ON498_001546"
     CDS             complement(15666..16448)
                     /locus_tag="ON498_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR3939803.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16445..17416)
                     /locus_tag="ON498_001547"
     CDS             complement(16445..17416)
                     /locus_tag="ON498_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3939804.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17508..18902
                     /locus_tag="ON498_001548"
     CDS             17508..18902
                     /locus_tag="ON498_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3939805.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         5329 a         3889 c         3366 g         6321 t
ORIGIN      
        1 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
       61 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
      121 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
      181 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
      241 ggccagcatt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
      301 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
      361 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
      421 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
      481 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
      541 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
      601 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
      661 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
      721 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
      781 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
      841 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
      901 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
      961 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     1021 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     1081 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     1141 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     1201 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     1261 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     1321 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     1381 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     1441 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     1501 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaaaaa tgaatctgct
     1561 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     1621 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     1681 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     1741 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     1801 acttgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     1861 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     1921 tttgcaaaga tagcttttgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     1981 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     2041 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     2101 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     2161 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     2221 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     2281 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     2341 ttgatcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     2401 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccaccac ctgttgagat
     2461 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcagctgagt ctccgccacc
     2521 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     2581 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     2641 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     2701 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     2761 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     2821 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     2881 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     2941 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     3001 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     3061 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     3121 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     3181 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     3241 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     3301 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     3361 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     3421 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     3481 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     3541 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     3601 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     3661 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     3721 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     3781 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     3841 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     3901 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     3961 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcaggaa
     4021 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     4081 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     4141 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     4201 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     4261 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     4321 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     4381 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     4441 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     4501 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     4561 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     4621 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     4681 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     4741 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     4801 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     4861 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     4921 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     4981 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     5041 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     5101 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcacg gtggttaccg
     5161 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     5221 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     5281 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     5341 ggaagtagtc ggtctagggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     5401 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     5461 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     5521 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     5581 tttttcaaaa gatctgtttc tgttcgcaat actcgttctg tgatccccat actttccgat
     5641 aaacttcgac gtccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     5701 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     5761 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     5821 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     5881 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     5941 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     6001 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     6061 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     6121 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     6181 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     6241 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     6301 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     6361 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     6421 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     6481 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     6541 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     6601 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     6661 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     6721 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     6781 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     6841 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     6901 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     6961 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     7021 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     7081 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     7141 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     7201 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     7261 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     7321 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     7381 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     7441 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     7501 aattggtgga cagttttttg ttgtacctgt atagatattg ttaagccttt gcaagtaagg
     7561 tagttatgtg ttcaactact tattaaaatg tgttatttat taaagatact tgtctcactt
     7621 actgaactgg ccgatgatcg tttctgccat aatagttctc gacagccgtt ggatgcccta
     7681 ataaatcccc aactgtcgtg atgcgatacc cttgctcttt cagataatcg atcacttgag
     7741 gaacggcacg aatcgtttca ggatagatat catggaataa aataattgcc ccatcgtata
     7801 ctgttgcttg gatcttctgc aaaatcaaat caggatttct tgtcttccaa tcttctgaat
     7861 caacagacca ttcgatgatc ggtcgctgga tgatcgttgc tcctacattc gtgacagaac
     7921 catagggcgg acgaacaaaa gtgggggttt tgccagtgac ttttttgatc tcagtgtctg
     7981 ctttcgtcac ttcctccgtc atcgactggg cagaaagagt ggccagattc tgatgatcgt
     8041 atgtgtgatt gccaatctcg tgccctgctt cagctgtctg cttcacgatt tcaggatgct
     8101 gcgccgtatt ttctcctaag acgaaaaaag tggcatgcac gtttttttct tttaagattc
     8161 gcagaagctg cggtgttgtg gtcgctcctg gtccatcatc aaagctgatt gcgacgcttt
     8221 tgacagccgg atctcggctc gtttttgtac tgtcttgtgt tgattgtgat gactgacttt
     8281 gtaccttttc cgctgattct tgaatggctt ctttgacgtt agctgtttca gttgattggc
     8341 ttttaatggt tgattgttct gattgtttga aagattgtga cgaatgctcc gaagagtgtg
     8401 tttgtccttt tacttcggtg atcaaggagg ccaccgaagt aatttcgaaa aacaaaagca
     8461 cggataaagc aataatactg ataactaaat aatttctttt cactatatct cttctttctc
     8521 tttttctctt cttattattt acttgtactt acttgaaaat tgaaactgct agtcgcaact
     8581 acttctcgtc taatgatcca aataatcttc cgatgccaaa atcgtttttg tcacggactt
     8641 aggaatgaca aattctggtg tacccatata accaggagca atttcgtatt gagaaaaagt
     8701 caaaactaat tgatgctctt tgttcaaata aaaactttgt gtaggcgtaa caagtgttgg
     8761 tttttctatc gtcccttctt gaatatcttg ctcagtccaa tagtacttag atggatcttc
     8821 cttgacttgt tcggccatct gccgtttgac ttcttttgtc aaaacagata gataggtgtc
     8881 atctttgaat aaaagtggaa gtgagagctg gacactgttt ttcttgtcga ttgtgtcgta
     8941 tcttttttca acatggctat ctgctgctgt ttgggtgatc tgcctttcaa tcacgagaaa
     9001 ccggttatcg tctacgattt tctgataatc tcccaaaact tggtaatgat ccgtttcaaa
     9061 atttccatat tctttttcag ctttttcaaa atctgcttgt ccttcgcgaa agtatttttg
     9121 atttaatcca tgaattgttt tattttcttt ctcattttgt gtattgatct tcggtacctg
     9181 aatatcgatt ttttcagatc gatcagtaaa gcgttcgcct aagatcaatt caatgactga
     9241 tcctaagatg ggtaaatctt ctgcaaagct tcgaacttga ctattaaaaa gaaggctact
     9301 tgttgtaatc attacgatag ccaaagatac tccgaaccct tggagacgtc tctttctccg
     9361 ctttgctttt ataaatgatt gctcttcgat aataaaacgc tggcggatct cctctttaag
     9421 ccctttggga attgcttgtt ctttataagt tttctttagt ttttttaaat tttctttttg
     9481 ggaatacatg gcactcttct ctccttcatt ctaggatttg acgtaaagca ttcaatgagc
     9541 ggtacatttt cgttttaact gtgttttcat tcatatccaa gatagtcgca atttctttca
     9601 aacgaaatcc ttcaaaaaag tagaggatca gaatctcttt ttccggactg tctattttat
     9661 ctaaaatcat atggagctct gcaacggaag tttctaaagg taactgttgg gggatatctt
     9721 gaatttcaaa taagtccaca ggcgtccttt tttgtctgcg ccaactatct aatgccgtgc
     9781 ggatcaatat ccgataaaac catgcactaa aatgctcgat ctctttttct ttttcaaaat
     9841 agttcagggc tttatgaaaa ctatcttgca tcacatctaa ggcctcttct ttatcatgta
     9901 cgtaattcat cgtgatacgg aacatttttt gataatcagt ttcgatgagt ccattcagca
     9961 ggtgacggta tttttcgatc tgccgttttt tcttatatcc agtaaaaatc attctccact
    10021 cctttctgcc tcacttaata tgacgtacaa ggtgcgctaa aagtttcaat ttttttattt
    10081 tttatgtatt tacttgggat ttttatatac aaaaaagaag gtgtgacaaa aatcgtgtca
    10141 cacctcctta ttctgatagg ctgatcgtct ctgtcacaac ctctcgtaag aaaataatgt
    10201 ttattctttt tcagagtcag aaaattcttc ctcttcataa tcttcatcat catcgtcttc
    10261 gtctatgata cttaaatctt cttcgatatt tcctggcaag tcttcttcat catctgtcgg
    10321 gtcatctgct ccgatttctt gcaagtcaga attataagca gcgatttctt catcttcatc
    10381 atcatcgtca tcaaacagat catcgtcatc ttcttctgtc agatctgtat cttctgggtc
    10441 atcgtcattg taatcgatcg cgtcttcatc atcattgata aaggcgttga ctttcttgcg
    10501 tttacggcga cgtggagtat cttcatcctc ttcgtccatt ccatggttta cttcttcatc
    10561 gatcgaatcg atagcatacc atgaacgcaa gccccaacga ttatccccta atgagataaa
    10621 gctgccgtca atattcaagt cagtataaaa ttgtgcgagt gaatcacgaa tttcactgtc
    10681 tgatttccca agatagtttt ggatctggtt agctaaatca gaaaaatcca tgacatcgcc
    10741 acgttgttct aagatcgcgt gtgctacttc gatcattgat aattcgcttt tgtttgcacc
    10801 ttcaaataca ctaatttcca atcaggacac gtcctttcac agtctactcc ttatcataca
    10861 aaatcagaca caaaaaatca aacttttctt ttatatttac gctgtaaaat ccaaggtcaa
    10921 ataatattcg ccgatttttt ccggtcccat gaacagatca taatcgattc gaaccttccc
    10981 tgctgtggga cggtctttca aactaacatg cagttctttc gtaaaagtag caatctggat
    11041 cataccgtat ggtgttcgat aggatgtttc taaccgttct ttatagccaa atttcaaacg
    11101 catccgcatt tctcctgcac ggatcagctg tacatgacca tctggaacta atttgatcgt
    11161 tacaggaatc tcatcgcccg tctcctcctg aatctcttta taacgtatgt acagcgtatc
    11221 gccaattgtc accatttgcc ctttcaaatc aaaataaaaa tcttgcactt ccccttgctg
    11281 ttttactttt gtacgcaatt tgattgaaat cggtgttcct tgtgataatt ccattattca
    11341 ttctccttta cttctcatag ctacattgtt cctagtttct tttgttttgt caaccaactt
    11401 gtccattttt acagaaaacc ttttttccaa aacaactctt tgattttaga gtataatatt
    11461 gtagaaaata tgaatttata caaaggagcc ggttgaatga accctgcgat tcgactagat
    11521 cagcacattt atcaaaaaaa cgacttttcc ccttttgcat taacagatat attaacggaa
    11581 tattctaaag aacaccagca aatcttttta catttctggc aatatgactg cacagtcatc
    11641 ttaggtatga aggatgttcg aactcctttt ctaagtgacg ggatccaaac actggaagat
    11701 gcaggttacg ctccagtgat cagaaattca ggtggattag ggatcgtatc agatgaagga
    11761 attttgaata tttctttgat tttcccccaa agtgacacaa aaaaaataac catagatgac
    11821 gcttacgagc aaatgctcct attgactaaa tacgcttttc caaaggcacc gatacaagca
    11881 ttcgaaatca cccattctta ttgtcctgga acttatgatt taagcatcca tggcaaaaaa
    11941 tttgccggta tcgctcagcg tcggatcaaa aatgggattt ctgttatgat gtacttaagt
    12001 gtcaacggta atcaggaaaa acgtgggcaa gtcatgaagc gattttatca atctgcttta
    12061 aaagaagact ttggtacaaa tggctatcct gcagtggatc ctgctacaat ggcaacactt
    12121 gaagaactgc ttgaccaacc cctttttgta aaagaagtca aagaagcatt tgcaaaagct
    12181 tttgaaaagc tgtattctgc aagtgattta caggattcta ccgactggct aaaaaaagaa
    12241 atccagctgg agcaatggaa caaccaaatc cagagaatgg aacaacgtaa taacataaag
    12301 gagctacatt atgacaaccc cttataaata tcaaatgctg cctatggaaa aagtttttcg
    12361 tgatccagtc cataactata tccatgtcca gcatcaagta atcttagatt tgatcaattc
    12421 gaaagaagtc caacgtctac gacgcattaa acaattaggc acttcttctt tcacttttca
    12481 cggagcagaa catagccgtt ttactcattc attaggcgtt tatgagattt caagacgaat
    12541 ctgcgatatc ttttctcgaa atttttcaaa agaaaaaata ggcaacggag gctgggatga
    12601 tcaggaacgg ctggtgactt tatgtgcggc actgttgcat gatgtcggtc atggtcctta
    12661 ctctcataca tttgaacata tcttccatac gaatcatgaa gccatcactg tagagatcat
    12721 cacttctcca gaaacagaag tgtatcagat tcttaaccga gtagaagaag gttttcctga
    12781 aaaggtagct agtgtgatcc aaaaaactta ccccaaccca caagttgtcc aaatgatttc
    12841 tagtcagatc gatgcagatc gaatggacta tttgcttcgg gatgcttatt tcactggaac
    12901 agaatatgga acatttgatc ttacccgaat attacgtgtg attcgtcctt atgaaggcgg
    12961 gatcgctttt tccatgagtg gcatgcatgc agtggaagat tatatcgtta gccgatatca
    13021 aatgtatgtg caagtgtact tccatcctgt ttcccgtggt atggaagtaa ttttggagca
    13081 tctgcttcat cgcgcacacg agttgtatca agaagatgcc agcagttttg aactccattc
    13141 tcaattactt ctgccatttc ttgacggaag ctttacttta gaagaatacc tcaaacttga
    13201 tgatggggtc ttgggcactt actttactca atggtctgaa tctgctgatc ctattttaaa
    13261 tgatttagct aaacgttttt tgaatcgtaa accactgaaa tctgctactt tttctggcaa
    13321 ccgggattca aaattagtcc aagaactcac tttgttagta gaaaaggtcg gatataatcc
    13381 tgtttattac actgctgtga attctagcta cgatctacct tatgattttt atcgcccaga
    13441 acaaggcaga catcgcacac aaatcgagat tttgcgaaat gacggaactt tgatagaatt
    13501 atcacaggtt agtcagcttg ttgctgcatt agcgggacaa gaacaaggag atgagcgatt
    13561 tttctttcca aaagaaatgg ttgatccgtc cttgcgtgat cattatgatt tatttgacga
    13621 aacttatcaa gaatttgcca gccatatccg taatggcgca ttgattgaaa taaattaaaa
    13681 tatattggag gaaattatgt ctatcaaact agtcgctatc gacatcgatg gtactttatt
    13741 aaatgagaaa agagaagtaa caaaagaagt aaaagaagcg attgccgctg ctgttgcaaa
    13801 aggtgtttcg atcgttttat gcactggccg tcccttacca ggtgtccagg aacagttaaa
    13861 cgagttgaac ctttttcaag ataatgatta cgttattact tataacgggg cactcgtaca
    13921 acaaacgaaa tcaggtaaaa tcatcgcccg tcacggactg acacatgaag atttcctaga
    13981 gatcgaagta atggcacgcc gtgtcggcag tcatctccat tcgatcgatg acaaagcgat
    14041 ctacacgcct aatcgagata tcagcgctta tacgatccac gaagcaagtt tagtcaagat
    14101 gccgctaaaa tatcgaacac ctgaagaaat gacaccagac atgaacatcg tcaaaatgat
    14161 gatgatcgac gaaccagaaa ttttagatgc agcaattgcg cgtttaccgc aaactttccg
    14221 tgataaatac acaacggtca aaagtgcgcc ctattacttc gaagtactaa ataaagaagc
    14281 aagtaaaggt gcggcagttg cgaatctcgc acagcacctt ggtattgatc aagatgaaat
    14341 catggctatc ggagataacg agaacgactt atccatgatt gaatatgctg gtctaggagt
    14401 agcaatggga aatgccgttt ctcttgtcaa agaagcagca aatgtcatca cttcatcaaa
    14461 cgacgagcac ggtgtagctg aagcaatcaa aaaatatgta ctgtaataaa taaaaaaact
    14521 gagagaaagc tgatctactc gctttctttc agtttttatt tttttcgatc catctctcga
    14581 ctgcccatcg gtggctacca cgtttcaatt tcacaagcgc ttcttgaggg ctcacccacc
    14641 aaattttatt ggtcttttca gttggttctc ctatacgctt ccaagaatca gcaacaaaga
    14701 aatatcctgg attataataa taagttgcac gataattcga gtaaaaatac tcatcagctt
    14761 ctcccagata agaagaaatt tctgccacaa ttcccatttc ttctatcagc tcacggacaa
    14821 tcgcttcttc tttcgtctct tctccttcaa tttccccgcc aggaagaaaa aaagcaccat
    14881 ttggagcctc gattactgca atctgctgat ctctttctat gacaatatat gctgcataac
    14941 gggtttgata atcagcaaat tgcttttttt caccaaaaat tggtttatcc atgaatagtt
    15001 tccaccttaa cagttttatt ttctatccat tttacatgag aaatttctca tgaacaagca
    15061 caaatcaact atttccttac ttctttttgt tatgattgag taaaaggaaa aggagagatg
    15121 aatctatgaa aatggctcac acatgtgttc gagtgaaaga tcttgaagca tcaatcgaat
    15181 tttatcaaaa ggcatttggt tttgaagaaa gccgccgccg tgattttcca gaaaacaaat
    15241 ttacacttgt ttatttgaca cttcctgggg atgactatga attagagttg acttacaact
    15301 atgatcatgg tgcgtatgat ctaggagacg gctatggtca tatcgctata gcagctgatg
    15361 atctagaaaa attacacgag caacataaag ctgctggtct tgagattact gacttaaaag
    15421 gattacctgg cactgcgcct tcttactact ttgtcattga ccctgatggt tataaaatcg
    15481 aagtcatccg aggatagtta gtatggggca ttttactctt gattgaataa aaaacaaaca
    15541 ccaataatta aaatgagacg caaacttttc attaaattga aaaagtttgc gtcttttttt
    15601 aacaaccatt gtagctataa ggttgccact ctccaccaat ttatttttaa ttcatttgaa
    15661 agaaatcaaa taaacgaggt acctttagaa aacaaaactg cttttcctcc tattatcaca
    15721 cgatcttctt ttaattcaca ttctagatcc ccgccccttg atgacatttg ataagctgac
    15781 aattttgttt tattcattct ttctgcccaa taaggaataa gattagaatg tgctgatcca
    15841 catactggat cttcattgat tgttaatttt ggaaagaagg ttctagaaac aaagtctttt
    15901 tgcacacttt ctgctgtcac gatgactcct attcctaatt caaaattttt gatagctgta
    15961 aaatctggct gcaatttagc caccgttttt tcatcttcta atacgaaaaa taaatcacgt
    16021 gctaagtagg cttctttaat cttagcccca atcgcttctt catattcaga caatatcggc
    16081 acttttttcg gcataatact aggaaaatcc atgtaataat aatctttaat tcttgtcaca
    16141 aatagattac cgctttgagt agaaaattta atcgtttcat caggtatttt gtaatagtta
    16201 aataaaacaa atgctgttgc taatgtcgcg tgaccacaaa gatcgatttc tcgatctggc
    16261 gtaaaccatc taagctcaaa ttcttggttt ttcttcactg taaatgctgt ttcagataaa
    16321 ttgttttcaa tggcaatttt ttgcatcgta ccttctggca gccattcttc tagcacataa
    16381 accgctgctg gatttccttt gaacacctca tctgcaaatg catcaactac gtaataatct
    16441 aagttcattt taaagcccct ttctctaatt tgttataaat atattctgca caacaaagat
    16501 cttcaatagc tagaccaact gcgtcgaaaa tcgtcggcct cgattctcct ttataaaatg
    16561 tttttttctc agaaactaat tcgcctaatg taccaacaat acaagattcc tctaaatctc
    16621 cttctgccat ggctataagc aggtccccgc tttctgttaa ggcagctgag taatcatcta
    16681 catagatttg agaagtttcc atcaacgatg tacttatctc tcttgtatca ggagtaaatg
    16741 tgcccacagc attgatatgt gcgtcttccc ttacccactc tttttgaaga aatgcctctt
    16801 tactagaggt caacgtacaa atgatttccg ctggacgtac tgcatcttct actgatattg
    16861 catttataaa cgttatttta ggatactgtt ttttagcttt agcgataaaa gtatttcgtc
    16921 tttcttctaa aatatcgtaa acaaacactg tagtgatggg acgaatggta gaaatcgcag
    16981 tcaaatgcga caatgcttgt tgaccagctc caattaaagc aagtgtttct gcatgcggat
    17041 ttgctaagta atctgttgcc agcgcagata cagcagctgt tcgaatccag gtgactgcat
    17101 ttgcgtttat caatgctttg ggtagtccgt tttgagcatc aaacaagaaa atctctccta
    17161 aatgagaagg taaatgatac tgatgattat ccggaaaagc ggtaatgact tttgtgccaa
    17221 aatatctgtt ctcccccaaa taagcaggca tcatcgcaaa catatttttg ccttttccgt
    17281 caggtaaaat ttccaccaat cgaggatttt ggctgatttt accactttca taatcaaaaa
    17341 aacatttttt cattacttga atcgcttctt caaaactaaa agaagctact acttcctcaa
    17401 aattaataat tttcatagac aattccctcc tcacaaaaga ttaacttcta aaatcaatac
    17461 aatcaatggt aaaattgtat ggtatacaat tttaaggagt ggagaaaatg cctattaatt
    17521 cttatgacaa ttacccgtta acttggcgac cagacaaaca aaaattgaag cgtccttatt
    17581 atcgatcgtt gattcaacaa ttagaacaag atattttaaa gaataaatta cagaaaaaca
    17641 ctcagttacc ctcccaaaga gagctagctg attatttaga tttaaatttt actaccgttg
    17701 gcaaggctta taaatatgga attgagaaag gtttgctgta tacgaatatt ggaagtggca
    17761 catttatttc tcccaatgtc tttgagtcca tcaccatctc taccaatgaa gtaaccgaac
    17821 atatcattga ttttggtctg gttagttctt ttgaagaatg taataaattc gttgtcccgt
    17881 ttattcaatc aactagtaat aacccaaatc ttgttggttt gcttaattac caagatccca
    17941 aaggcacaaa ttttcaattg actactgcag caaattggtt aaaaactcaa ggagtcattc
    18001 cgtccttaga atctctatct attgtttccg gtgttcaaaa tggattggct atcacacttt
    18061 ctgcactttt ctctccaggt gatagaatag caacagaccg atatacatac gctaatttca
    18121 ttgaattggc tcagttatat catttagaga tcgttccaat tgattttgac gagcaaggaa
    18181 tgcgacctga tatattgatt caggaatgta gaaaaaagaa gattcatggt gtctttctta
    18241 tgccttcttg taataatcct atagggtttc aaatctctca caaaagacga gagcaattag
    18301 ccgaagtgat tgaaaaagaa ggtctatggg ttattgaaga tgatatccat tcatttttaa
    18361 caacttacta tcaacatacg atcattcctc ctttccaaaa tttattacct aaccaaacaa
    18421 tctacttagc tggaatgaca aaatttattt gttctggatt gcgagttgct tatcttgtag
    18481 ttccagtaca tgtccaaagc aagatagaac aagcaatttt taatataaat gtgaaaactt
    18541 ctggattaga tgtggaagtc atcactcaaa tcctccattc aaatgaagca aataggatct
    18601 tagaaactaa attaagtctt acaaaaagag ccaatcagct ttttgattct cttttcaaag
    18661 tagaaaaacc aacgaatcgt gtcccttact atcgaattct tccagttgat cctgagattg
    18721 accctaagat aattgagaat gactttcttg ctaacggtat ccgactttat cattccagac
    18781 gctttactac acgtgcgcaa ccagatgcat tcgttcgaat ttccctatcc tctaataacc
    18841 ttgaaatttt agcttctgga ctaagtatcg taaaagaaaa aataaaaaaa tatgaattat
    18901 aacct
//
LOCUS       DAJSVD010000049        18416 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSVD010000049 DAJSVD010000000
VERSION     DAJSVD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.050"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            171..515
                     /locus_tag="ON498_001549"
     CDS             171..515
                     /locus_tag="ON498_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3939806.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ON498_001550"
                     /pseudo
     CDS             516..935
                     /locus_tag="ON498_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ON498_001551"
     CDS             complement(1027..1377)
                     /locus_tag="ON498_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939807.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ON498_001552"
     CDS             complement(1343..2674)
                     /locus_tag="ON498_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR3939808.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="ON498_001553"
     CDS             2930..3496
                     /locus_tag="ON498_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3939809.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="ON498_001554"
     tRNA            3641..3728
                     /locus_tag="ON498_001554"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /gene="bsh"
                     /locus_tag="ON498_001555"
     CDS             4062..5036
                     /gene="bsh"
                     /locus_tag="ON498_001555"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3939810.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="ON498_001556"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="ON498_001556"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR3939811.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="ON498_001557"
     CDS             complement(7451..7897)
                     /locus_tag="ON498_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3939812.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="ON498_001558"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="ON498_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3939813.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="ON498_001559"
     CDS             8362..8880
                     /locus_tag="ON498_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3939814.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="ON498_001560"
     CDS             complement(8957..9913)
                     /locus_tag="ON498_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3939815.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="ON498_001561"
     CDS             10079..10885
                     /locus_tag="ON498_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3939816.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="ON498_001562"
     CDS             10882..11871
                     /locus_tag="ON498_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3939817.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="ON498_001563"
     CDS             11868..12872
                     /locus_tag="ON498_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3939818.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="ON498_001564"
     CDS             13004..13546
                     /locus_tag="ON498_001564"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3939819.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="ON498_001565"
     CDS             13566..14264
                     /locus_tag="ON498_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3939820.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="ON498_001566"
     CDS             14285..14497
                     /locus_tag="ON498_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR3939821.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="ON498_001567"
     CDS             14497..15459
                     /locus_tag="ON498_001567"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR3939822.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="ON498_001568"
     CDS             15477..15872
                     /locus_tag="ON498_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3939823.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="ON498_001569"
     CDS             16035..16400
                     /locus_tag="ON498_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR3939824.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="ON498_001570"
     CDS             16397..18208
                     /locus_tag="ON498_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR3939825.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SCSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18416)
                     /locus_tag="ON498_001571"
     CDS             complement(18270..18416)
                     /locus_tag="ON498_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR3939826.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
BASE COUNT         5916 a         3118 c         3808 g         5574 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaaaaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attgttcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 catacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcagc caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatat cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt aagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga tcttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaatcga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaatga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tcttgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatat
//
LOCUS       DAJSVD010000050        17687 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSVD010000050 DAJSVD010000000
VERSION     DAJSVD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.051"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            74..904
                     /locus_tag="ON498_001572"
     CDS             74..904
                     /locus_tag="ON498_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR3939827.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1148..1466)
                     /locus_tag="ON498_001573"
                     /pseudo
     CDS             complement(1148..1466)
                     /locus_tag="ON498_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1588..1782)
                     /locus_tag="ON498_001574"
     CDS             complement(1588..1782)
                     /locus_tag="ON498_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939828.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2287..2472)
                     /locus_tag="ON498_001575"
     CDS             complement(2287..2472)
                     /locus_tag="ON498_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939829.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3322..3663)
                     /locus_tag="ON498_001576"
     CDS             complement(3322..3663)
                     /locus_tag="ON498_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3939830.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3663..3893)
                     /locus_tag="ON498_001577"
     CDS             complement(3663..3893)
                     /locus_tag="ON498_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3939831.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4180..4518
                     /locus_tag="ON498_001578"
     CDS             4180..4518
                     /locus_tag="ON498_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939832.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4723..4962)
                     /locus_tag="ON498_001579"
     CDS             complement(4723..4962)
                     /locus_tag="ON498_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3939833.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(4938..5285)
                     /locus_tag="ON498_001580"
     CDS             complement(4938..5285)
                     /locus_tag="ON498_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3939834.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(6017..6598)
                     /locus_tag="ON498_001581"
     CDS             complement(6017..6598)
                     /locus_tag="ON498_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3939835.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6781..7407)
                     /locus_tag="ON498_001582"
     CDS             complement(6781..7407)
                     /locus_tag="ON498_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3939836.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7429..8760)
                     /locus_tag="ON498_001583"
     CDS             complement(7429..8760)
                     /locus_tag="ON498_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR3939837.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8915..9316)
                     /locus_tag="ON498_001584"
     CDS             complement(8915..9316)
                     /locus_tag="ON498_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3939838.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9402..9608)
                     /locus_tag="ON498_001585"
     CDS             complement(9402..9608)
                     /locus_tag="ON498_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939839.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9703..11205)
                     /locus_tag="ON498_001586"
     CDS             complement(9703..11205)
                     /locus_tag="ON498_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR3939840.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11223..11678)
                     /gene="argR"
                     /locus_tag="ON498_001587"
     CDS             complement(11223..11678)
                     /gene="argR"
                     /locus_tag="ON498_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3939841.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11776..12393)
                     /locus_tag="ON498_001588"
     CDS             complement(11776..12393)
                     /locus_tag="ON498_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR3939842.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12614..13987
                     /locus_tag="ON498_001589"
     CDS             12614..13987
                     /locus_tag="ON498_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939843.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14062..15174
                     /locus_tag="ON498_001590"
     CDS             14062..15174
                     /locus_tag="ON498_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3939844.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15246..16883)
                     /locus_tag="ON498_001591"
     CDS             complement(15246..16883)
                     /locus_tag="ON498_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR3939845.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17051..17659)
                     /locus_tag="ON498_001592"
     CDS             complement(17051..17659)
                     /locus_tag="ON498_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3939846.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4989 a         3619 c         3105 g         5974 t
ORIGIN      
        1 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
       61 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
      121 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
      181 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
      241 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
      301 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
      361 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
      421 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
      481 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
      541 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
      601 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
      661 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
      721 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
      781 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
      841 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
      901 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
      961 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
     1021 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
     1081 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
     1141 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
     1201 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
     1261 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
     1321 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
     1381 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
     1441 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
     1501 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
     1561 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
     1621 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
     1681 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
     1741 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
     1801 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
     1861 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
     1921 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
     1981 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
     2041 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
     2101 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
     2161 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
     2221 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
     2281 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
     2341 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
     2401 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
     2461 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
     2521 aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac
     2581 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
     2641 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
     2701 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
     2761 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
     2821 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
     2881 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
     2941 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
     3001 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
     3061 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
     3121 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
     3181 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
     3241 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
     3301 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
     3361 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
     3421 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     3481 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     3541 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     3601 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     3661 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     3721 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     3781 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     3841 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     3901 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     3961 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     4021 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     4081 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     4141 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     4201 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     4261 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     4321 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     4381 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     4441 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     4501 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     4561 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     4621 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     4681 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     4741 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     4801 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     4861 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     4921 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     4981 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     5041 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     5101 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     5161 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     5221 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     5281 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     5341 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     5401 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     5461 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     5521 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     5581 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     5641 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     5701 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     5761 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     5821 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     5881 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
     5941 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
     6001 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
     6061 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
     6121 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
     6181 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
     6241 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
     6301 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
     6361 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
     6421 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
     6481 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
     6541 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
     6601 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
     6661 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
     6721 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
     6781 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
     6841 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
     6901 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
     6961 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
     7021 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
     7081 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
     7141 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
     7201 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
     7261 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
     7321 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
     7381 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
     7441 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
     7501 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
     7561 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
     7621 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
     7681 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
     7741 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
     7801 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
     7861 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
     7921 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
     7981 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
     8041 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
     8101 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
     8161 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
     8221 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
     8281 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
     8341 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
     8401 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
     8461 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
     8521 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
     8581 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
     8641 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
     8701 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
     8761 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
     8821 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
     8881 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
     8941 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
     9001 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
     9061 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
     9121 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
     9181 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
     9241 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
     9301 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
     9361 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
     9421 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
     9481 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
     9541 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
     9601 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
     9661 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
     9721 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
     9781 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
     9841 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
     9901 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
     9961 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
    10021 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
    10081 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
    10141 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
    10201 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
    10261 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
    10321 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
    10381 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
    10441 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
    10501 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
    10561 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
    10621 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
    10681 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
    10741 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
    10801 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
    10861 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
    10921 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
    10981 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
    11041 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
    11101 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
    11161 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
    11221 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
    11281 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
    11341 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
    11401 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
    11461 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
    11521 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
    11581 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
    11641 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
    11701 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
    11761 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
    11821 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
    11881 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
    11941 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
    12001 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
    12061 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
    12121 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
    12181 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
    12241 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
    12301 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
    12361 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
    12421 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    12481 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    12541 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    12601 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    12661 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    12721 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    12781 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    12841 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    12901 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    12961 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    13021 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    13081 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgtt
    13141 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    13201 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    13261 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    13321 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    13381 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    13441 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    13501 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    13561 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    13621 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    13681 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    13741 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    13801 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    13861 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    13921 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    13981 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    14041 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    14101 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    14161 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    14221 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    14281 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    14341 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    14401 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    14461 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    14521 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    14581 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    14641 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    14701 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    14761 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    14821 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    14881 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    14941 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    15001 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    15061 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    15121 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    15181 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    15241 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    15301 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    15361 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    15421 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    15481 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    15541 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    15601 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    15661 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    15721 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    15781 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    15841 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    15901 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    15961 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    16021 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    16081 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    16141 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    16201 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    16261 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    16321 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    16381 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    16441 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    16501 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    16561 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    16621 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    16681 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    16741 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    16801 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    16861 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    16921 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    16981 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    17041 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    17101 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    17161 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    17221 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    17281 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    17341 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    17401 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    17461 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    17521 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    17581 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    17641 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gtttttt
//
LOCUS       DAJSVD010000051        17546 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSVD010000051 DAJSVD010000000
VERSION     DAJSVD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.052"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            165..2699
                     /gene="secA"
                     /locus_tag="ON498_001593"
     CDS             165..2699
                     /gene="secA"
                     /locus_tag="ON498_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3939847.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2825..3926
                     /gene="prfB"
                     /locus_tag="ON498_001594"
     CDS             join(2825..2896,2898..3926)
                     /gene="prfB"
                     /locus_tag="ON498_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3939848.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4031..4717
                     /gene="ftsE"
                     /locus_tag="ON498_001595"
     CDS             4031..4717
                     /gene="ftsE"
                     /locus_tag="ON498_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3939849.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4701..5594
                     /locus_tag="ON498_001596"
     CDS             4701..5594
                     /locus_tag="ON498_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3939850.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5746..7194
                     /gene="cls"
                     /locus_tag="ON498_001597"
     CDS             5746..7194
                     /gene="cls"
                     /locus_tag="ON498_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3939851.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7531..8388
                     /locus_tag="ON498_001598"
     CDS             7531..8388
                     /locus_tag="ON498_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3939852.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8503..9423
                     /gene="pstC"
                     /locus_tag="ON498_001599"
     CDS             8503..9423
                     /gene="pstC"
                     /locus_tag="ON498_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR3939853.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9423..10307
                     /gene="pstA"
                     /locus_tag="ON498_001600"
     CDS             9423..10307
                     /gene="pstA"
                     /locus_tag="ON498_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3939854.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10322..11128
                     /gene="pstB"
                     /locus_tag="ON498_001601"
     CDS             10322..11128
                     /gene="pstB"
                     /locus_tag="ON498_001601"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3939855.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11144..11902
                     /locus_tag="ON498_001602"
     CDS             11144..11902
                     /locus_tag="ON498_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3939856.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11915..12592
                     /gene="phoU"
                     /locus_tag="ON498_001603"
     CDS             11915..12592
                     /gene="phoU"
                     /locus_tag="ON498_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3939857.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12815..13846
                     /locus_tag="ON498_001604"
     CDS             12815..13846
                     /locus_tag="ON498_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3939858.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13843..14610
                     /locus_tag="ON498_001605"
     CDS             13843..14610
                     /locus_tag="ON498_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3939859.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            14710..16278
                     /gene="liaX"
                     /locus_tag="ON498_001606"
     CDS             14710..16278
                     /gene="liaX"
                     /locus_tag="ON498_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR3939860.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSVIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16454..16762
                     /locus_tag="ON498_001607"
     CDS             16454..16762
                     /locus_tag="ON498_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR3939861.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            16762..17112
                     /locus_tag="ON498_001608"
     CDS             16762..17112
                     /locus_tag="ON498_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3939862.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17250..>17546
                     /locus_tag="ON498_001609"
     CDS             17250..>17546
                     /locus_tag="ON498_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3939863.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         5780 a         3017 c         3809 g         4940 t
ORIGIN      
        1 tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag ttcttacttt
       61 aatacgctta agatatttca attatctata actaatgata aaatgaaaag taaagtctac
      121 atagtctgga taatacacag aaggaaaagg agattacaca acgcatggcc aatttgattc
      181 gttcacttat cgaaaacgat aagaaagaac taaaacgttt agatagcatt gcaaaaaaag
      241 ttgaagcata cgctgaccaa atggcagcat taactgatga acaattacag gcaaaaactg
      301 ctgaattcaa gggcagatac caaaaaggag aaacattaga tcaattactt ccggaagctt
      361 ttgcagtcgt ccgagaagca gcaaaacgtg tattggggtt atatccatac cacgtacaat
      421 tgatgggtgg aatcgtgctt cacgatggaa atatccctga aatgcgtact ggtgaaggta
      481 aaaccttgac cgcaacgatg ccagtatatc taaatgctct gactggcgaa ggggtacacg
      541 tagttaccgt caatgaatat ttagcaaccc gtgactcgac ggaaatgggc gaactttata
      601 atttcttagg attaacagtg gggttgaata tcaactcaaa atcttcggat gaaaaacgcg
      661 aggcttataa ttgtgatatc acttatagta cgaataatga attaggattt gactacttga
      721 gagacaatat ggtcgtttac cgccatcaaa tggtacaacg tccgctgaat tatgccattg
      781 tcgatgaagt tgactcaatc ttgattgatg aagcgagaac gccgctgatc atttcagggc
      841 aagcagaaaa atccacagct ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag
      901 aagaagaaga ttataaaatc gatgtacaat caaaaacaat cagtttaacc gaagcaggga
      961 tcgaaaaagc agaagaaaca ttcggtttgg ataacttata cgacattgaa aatactgcat
     1021 tgacccatca cttggatcaa gcattgagag ccaactatat catgctttta gatatcgatt
     1081 atgttgtcca agacggaaaa gtactgattg ttgaccagtt cactggtcgg atcatggatg
     1141 gacgccgtta ttcagatgga cttcaccaag caatcgaagc taaagaaggc gtagagatcg
     1201 aagacgaaac gaaaaccatg gcgacgatca ctttccagaa ctatttccgt atgtacaaga
     1261 aacttgcggg aatgactggt acagctaaaa cagaagaaga agaattcaga gaaatttata
     1321 acatccaagt ataccagatt ccgacaaatc gtccggttat ccgtgatgat cgtccagatt
     1381 tactttaccc aacattacaa agcaagttcc acgctgtcgt gcaagatatc aaagatcgct
     1441 accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt tgaaacatct gagttattat
     1501 cagatatgct gaatcaagaa cgtattcctc atgaagtgct gaatgcgaaa aatcacttta
     1561 aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca
     1621 tggctggtcg gggaacagat atcaagttag gacttggggt aagagaactt ggtggtctag
     1681 cagttatcgg gacagaacga catgaatctc gtcgtatcga taaccagctt cgaggacgtg
     1741 caggacgtca aggagaccct ggtatgtcac aattctactt gtcattggaa gatgatttga
     1801 tgaaacgatt cggttcagaa cgaatcaaag ctttcttaga tcggatgaag atcggtgagg
     1861 aagatgctgt tatccaaagc aagatgctga cgaaacaagt agaatctgca caaaaacgtg
     1921 tagaaggaaa taactatgac acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg
     1981 aacaacgtga agtaatctat aaacagcgac aagaagtaat catggaagaa aaaagcttat
     2041 ccaagccact aatgaacatg gtcaaacgga caatcagtcg tgtagtagat gcgcatacac
     2101 aattagagaa agaaaattgg aatttggaaa gtatcatcga ttttgccggc agtactcttg
     2161 tccatgaaga tagtattact cttgcagata tcgaaggaaa aacatctcag gagatcaaag
     2221 agtatctaaa cgaacgagca caagaagtat ttaataccaa agcggctcaa ctaaatggtc
     2281 cagagcaact gttggaattt gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga
     2341 cagaccatat tgatgcaatg gatcaattac gtcagtcaat cggcttacgt gcttatggac
     2401 agaacaatcc attagtcgaa tatcaaacag aaggctataa gatgtttgaa gatatggtag
     2461 gcgccatcga gtatgaagtc actcgaatct ttatgaaagc tgagatccgt caaaacgtac
     2521 agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga agacggagat gccaaagaaa
     2581 atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa caaggtcgga agaaatgatc
     2641 cttgtccttg tggaagcggg aaaaaatata agaactgtca tggaaaaaat gcaaactaat
     2701 ttctgacagc aggattggtg agacaaattc tttttttaag aattcgtctc accttctttc
     2761 tgttatttgc tgattctgtt agactagata taatgaaaaa agtatagtga aagaaggaaa
     2821 aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag
     2881 tttcaggagg tctctttgac ttagatcagc tggaagaaga catcgcggaa gcagaagcaa
     2941 aaatggctga acctggtttt tgggatgaca gcgaagccgc acaaaaagtg atcagcgaaa
     3001 ataacgctaa taaagaaacc tatgaccaat tccattctct tgccgaagaa ctagaagaaa
     3061 tcgaattgat ggtggagatg ctgcaagaag aacctgatgc agcaatgcaa gaagaagcag
     3121 aaacacgtat caatgcatta gatgaaaaaa tgaagacgta tgaattatct atgctgttag
     3181 acggacctta tgatcgaaac aatgcgatcg tcgaattgca tccaggggct ggcggaacgg
     3241 agtcccaaga ctggggcagc atgcttttac gtatgtatac acgttgggca gagcagcatg
     3301 gctatcaagt agaaacacta gattatcaag caggtgatga agcagggatc aagagtgtga
     3361 ccctgctgat taaaggacat aacgcttttg gttatctaaa atcagaaaaa ggtgttcatc
     3421 gccttgtacg gatttcgcct tttgactcag cgaaacgacg tcatacttct ttttgttcgg
     3481 tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca gatgatttga
     3541 aaatcgatac gtaccgagca agtggtgcag gtggtcagca catcaataaa acagaatctg
     3601 ctgtacggat cactcacttg cctacaggaa cagtagtagc tagtcaggca caacgctcac
     3661 aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc taagctttat caattagaag
     3721 tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga acaaaaagaa atcggctggg
     3781 gttcacagat ccgttcttat gttttccatc cctattctat ggtaaaagac catcggacaa
     3841 acgtagagac aggaaatgtc caagcagtta tggacggtga ccttgattta tttattgacg
     3901 gctatttaaa aatgcatcta caataagaac tcattgttaa attgaaataa aattgtaaga
     3961 ttttgcaagc atgttgaaac attgccatgc tataatagct aaagactgaa aaagatatgg
     4021 agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc
     4081 tatccgcaat ctctcgattg tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc
     4141 tggagccggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg
     4201 aactttgaac gtcgcaggtc atgatctaat agcaatcaaa aaccgcgaag ttccttatct
     4261 acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa aaacagtata
     4321 tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa cctcgagata tcaaaaaacg
     4381 agtaatggaa gtgctggacc ttgttggttt gaaacacaaa gttcgagtct ttcctagcga
     4441 attatccggt ggggaacagc aacgtgtctc tattgctcgg gcaatcgtga atacgcctaa
     4501 agtacttatc gcggatgaac cgacaggaaa tctggatcca gaaaactcat gggaaatcat
     4561 gaaattatta gatcggatca atgcccaagg aactactgtc gtgatggcta cacataacag
     4621 tacgatcgta aatacgattc ggcaccgagt aattgctatc gaaaacggcc gcattattcg
     4681 agatcaagcg gaaggggaat atggatacga tgattagaac ctttttccgc catttattag
     4741 aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa
     4801 cgatcacgtt gactttagtc ggtgtattca tggcagttat catgaatgca actaaattag
     4861 ctcaggatat agaaggaaac gttgatgtat ctgtattcgt agatatcggt acaaaagacg
     4921 aagagatgaa aaagctagaa aaagaattga atgacttaga tcatgttaaa aaagttgaat
     4981 tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat
     5041 tgtttgaagg tgacaataac ccgttatatg atgtgtatgt agtaagtgct actgatcctt
     5101 cttatacaaa acaagtagca gaagaagcag cagatttgaa gaatgtttct cgttctgatt
     5161 acggtggagc ttcctcagat cggattttcc aaatcgctgg tgcagttcgg acatggggat
     5221 tagttgcagc ggcattgctg ctgttcgtag cgatgtttct catttcgaac acgattcgga
     5281 tcacgatttt gtctagacag cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg
     5341 gatatattcg ctggccgttt ttccttgaag gtggctggat cggtctgatc ggtgcgattc
     5401 ttccgatttt gatcatcaca tttggttatg catggttttt ccaattgatc aatccatcat
     5461 tattacgttc ccattattca ttgattcgtc cacaaaatat cgtttggaaa atcaatcttt
     5521 taatggtcgg cattggtgtg atcattggtt cacttggctc gatcatttct atgagacgtt
     5581 ttttgaaatt ctaataccac aatataaata aaatgagaaa gttggacagt ttcgttccag
     5641 ctttcttttt ttattggcta ggagaataga aggaagcggc ttttttccct ttactttaaa
     5701 aaacaacttg ggtattctta agttggtaga agaagaggtg aagccgtgaa aattatcgtt
     5761 gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat catcgttttt
     5821 cgagaaagaa aggaaactgc tcaaacatgg gcatggctgc tcgttttgat gttcattcca
     5881 gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga gaaaatcttt
     5941 gatttgaaga tccaagggaa aatcggtaag aacctggaaa ttgaagaaga caaacaggca
     6001 ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa acaattgatc
     6061 tatatgctca cggtatttga aagtacgctc tatacgacta aaaatgaaat cactttattt
     6121 acagatggtc gtgaaaaatt cgatgcgctg attgaagata tcaagcaggc agaagaccat
     6181 atccacttcc agtattatat ttatcgcagt gatgcattag gtgaagaagt tcgagatgca
     6241 ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc
     6301 acacaagttt cttccagctt ttttcagaat ttgaaaaaag caggcggaga agtcgcattt
     6361 ttctttcctc tatttgttcc gtatattaat cctcggatca attatcgcaa tcatcgtaaa
     6421 atcgtagtta ttgatgggaa aatcggatat acaggtgggt tcaatgttgg aaatgaatat
     6481 ctaggattgg taaagaaatt tggttattgg cgagacaatc atttgcgtat ttacggagaa
     6541 gcggtgtata ccttgcaaaa ccgttttttg atggactgga attctcagca tacaaaagaa
     6601 gtaggctatt ctcccaaatt tttcccgtcc attcacacta ctggtgatat agctgttcaa
     6661 atcgtgacaa gcggaccaga taccgaacat gaacaaatca aaatgactta tttgaaaatg
     6721 atttcgttag ccaaacggga gatattgatc caaacgcctt attacatacc ggatggatcg
     6781 attcatgaag cactgaagtt ggctcttctt tcaggcgtaa aagttcatat ccaaatacct
     6841 aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc agagctgatc
     6901 gagtatggag caaggatcga aacatatgag aatggattta ttcatgcaaa gacgatgatt
     6961 atcgatggtg gcattgtttc agttggctca gcaaatatcg atgttcgttc atttcgcttg
     7021 gactttgagg tcaatacact ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct
     7081 ttttttgaag actcgaaaat ttctacgcat ctcacaaaag aaatgtatga aaaccgagga
     7141 ataatcatca agatgaaaga aggattagca agattgattt cgccgctcct ttaaaacata
     7201 aaaaggacat gagaaccatc aacagatgga gatctcgtgt ccttttctaa cggggccatt
     7261 tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa ctttggggta
     7321 tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa agagacacat
     7381 ttcaaagcca gtatagcata gaaactttta atgagatagt tttttttaca attatgagaa
     7441 tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata
     7501 aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt cttaccaata
     7561 ataggattag ccattctttt atcaggctgt tcaaagtgga ttgatcgagg agaatccatt
     7621 acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga aacttaccag
     7681 acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac tggtttgtct
     7741 caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga agaaaaatcg
     7801 gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat cactcctatt
     7861 gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa actatttaca
     7921 ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt tatattgaac
     7981 cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga cggagaaaca
     8041 gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt tgccgacaca
     8101 cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga tacgttgaat
     8161 gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac gatttgggca
     8221 tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt tttagactat
     8281 atcttatctg atgaagtaca agataacatc gttggagaac taggttatat ccctgtttct
     8341 catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc tttacacaaa
     8401 atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt tcaatttgta
     8461 cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa
     8521 gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt catcagtttt
     8581 ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt agctagtaaa
     8641 ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt tggtacagat
     8701 tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt gcctatgatc
     8761 acaggttcgt ttattgtgac ctttttatct gctatcgttg ccacgccatt tgccattggt
     8821 gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt acagccggtc
     8881 atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt atcagttatc
     8941 gtgccttttg tcagaagtat ttttggcggg acagggtttg gtatcttagc tggtacattt
     9001 gtgttgttcg ttatgatctt gccgactgtc acaacgatga ctgttgatgc attaaaggca
     9061 gtaccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg gcaaacaatc
     9121 tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt ttttggtatg
     9181 gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc agcattgatg
     9241 ccaacaggac ttactactcc tgcttctaca ttgacgtcca tcttaacaat gggaatcgga
     9301 aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct gatcttgcta
     9361 ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa
     9421 taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatata gtttcgggga
     9481 tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga ataccgcaca
     9541 tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggtggt ggaatcggga
     9601 tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt tttcctattt
     9661 ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggcta acagatgtga
     9721 ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt
     9781 tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt gcattggctt
     9841 tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta caagcggtcc
     9901 attacacaca acgagaagca ggattggcct taggtttgtc acgctgggaa acagtgatga
     9961 aagtggtggt tccagaagcg acaccaggga ttttgactgg tgtgatccta agttctggaa
    10021 gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca cctgcgttag
    10081 attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc cgtcaagctg
    10141 aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct gatggtgttt
    10201 ctgtttcggc tggcgcttct gccgtgttga tcattgctgt tttactgttc aacttcggcg
    10261 ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga gaagaagcaa
    10321 aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt
    10381 agcactatat acggatgata tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt
    10441 ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg gctgcggaaa
    10501 atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata cgaaaatcac
    10561 tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg tttatgagat
    10621 gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt cgatttatga
    10681 caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat
    10741 cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa
    10801 aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgcattgctc gtgcgatcgc
    10861 gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc ctatttcgac
    10921 aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca tcatcgtgac
    10981 gcataacatg caacaagcag ctcgtatcag cgactatact gcctttttct atctaggaaa
    11041 agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc aggcaaccga
    11101 agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa aagaaattat
    11161 ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag ggatcgatct
    11221 aagtttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg gaaaatccac
    11281 ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga tcaccggaag
    11341 tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg aactaagaaa
    11401 agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct atgaaaatgt
    11461 catctatgga ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc aagtagttga
    11521 agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc
    11581 tttatcttta tccggcgggc agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa
    11641 tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt ctactgggaa
    11701 aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg tgacccataa
    11761 tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg gtcacttgat
    11821 cgaatacaac gatacgaaga agatattcat gaatccagaa aaacaagaga cagaagatta
    11881 tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga
    11941 attgctgaat ctccataatc agttctacga aatggggatg atggtgagca gcgccgtgca
    12001 caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag ttatcgaaaa
    12061 cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg aaatgatcgc
    12121 attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga aagctagctc
    12181 ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa
    12241 agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg attatgtgaa
    12301 aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac
    12361 gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata atgcaacaat
    12421 caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact tgaatgttgc
    12481 cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga tcgtctactt
    12541 ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct aaactgaagc
    12601 tgtgattatg ccgtttgttt atgaaaagag gttgtgaaaa agctgttcta catcaagtaa
    12661 taagaacagt caaacaaaaa atagctcctc atatttttcg tttggctgga cttattaccg
    12721 cagatgcaag cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa acatgtcaca
    12781 aacccttttc atcatttcat agaaaggata aagaatgcaa aaaaaaatta gcatcatcac
    12841 tatatttttt atttgtgtgc ttacttcatg ggttattcag catcaagccg ctttatatga
    12901 agtacctgtc atcgagatag aaaaagtacg aataaaagag gatcatcaat tgattacagg
    12961 aaaactgatg aactcggatc aaaaaggaga atcgatttat ttatcggttc cttatcaaaa
    13021 aaatcaaata gaaacagcaa atataaaagc gagacaaatg tatctagtcc aaatgcatgg
    13081 agatgaatgg cagctagtag caaaaaaaaa tgatggctgg cttttcttct tcacaagtat
    13141 cttcattggc gccatgttgc ttattggcgg aaaacagggt ctatttgctt taggaagtct
    13201 catcatcaat atcgttttac ttttactttt gttgctcatc tttactaaaa cagaagggat
    13261 ttctctatta aataaatcta tcctgttcgt cgttataggg atgattatta gcatattggc
    13321 tttagatgga tggaatagaa gcagtataat caaaatttca gctgcagtta cgagtgtttt
    13381 tttagcattt tttatttgtc ttttgacaat gaatcattgg aaagaccaag gactgcgcta
    13441 tgaggaactg aattatctga cacgaccgta tcgttctgtt tttctttcta gcgtactgat
    13501 tggaacagct ggaggaacac tcgataccgt tatcacagtc attgcgacat tagaagaatt
    13561 gactaagcga caaccagaaa taacagcgaa acagttatgg ctttcaggaa ggcaagtggg
    13621 tcgagatgtg attggctcga tgaccaatat ccttttgttc gtctatatca gtgggtcagt
    13681 tccatcgctt cttttatatt taggaaatca atggtctttc aaagaaacga tagaacagca
    13741 tttatcattg gaatttttac gcgtcattgc tggcagctta ggaatagtat tatcgatccc
    13801 catctcggtc ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt acttattata
    13861 cttgttggga ttttagcaat cagcgtgcta cttattggcg gtacgcaagg tatgtatatt
    13921 cttttaggct tattcatcaa tttagggatc tttttcctgt tgttattcgg ttatcatcag
    13981 aagtggccga tactggcatt gtcggtcata ggatttttat tgattgctgt ggttatctta
    14041 ttttttatca acggttataa tctaaaaatg cgtgcagctt ttgtgagtat actgatcttt
    14101 ttattttgtt tcctactatt gcttcctatc actgattttt tagcgatcca aggttttaca
    14161 tcggtagaac tagaagaatt atctggctta gataagactt tagcgattga ttttagatta
    14221 cttacgagat cgttgcttct gattagtctt agtggtgctg ttttagatgc gagtgtagca
    14281 atttctagtg ggacattcga agtatatcag gcaaatccgc aattatcttt ttaccaattg
    14341 cgccattcta gtttcgctat tgctaaaaaa atattggcgt caacagtaat gacacttctt
    14401 tttgcattca tgggaagttc tttagcgttg atcctctggt ttattgattt agacattcct
    14461 tttcagcaag tgatcaatga aaaatcattt gttctagaat atacgatggc tattcttacc
    14521 acattgtccg cactaattgt tcttccttta acctgcgtga ctacagcata tctctttaca
    14581 aaaaagaaag aacaactcaa gatggagtaa aagttacaac ttaagtccta tgacattttt
    14641 actgaatcag tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag
    14701 gcaatttcca tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg aatcctttcg
    14761 actgaagaag gcttagattt attagaaagc atggcaactg aaaaagacga aaagcaaatc
    14821 aaaaaagaag cagacaaggt aactgctagt cataaagaaa aagataaggc aagtcaattg
    14881 atcgataaac ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc aaaggaaaga
    14941 gaaagacaag atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat
    15001 aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa ggcagcacgc
    15061 aatgagaaac aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca
    15121 gaagaagaat tagcacagcg tcaggcgtta gaggcagaaa tcaaagagtt agaagctagt
    15181 ggcgatacac tgttagaaga acaattgaaa ttagaagcag aattaaaaga tatccgtaag
    15241 aatcaatggt ctgagaaaaa agaaacgttt actgataagt ttgaattgcc agatgattgg
    15301 aaagatcaag caacagatac actgaatcaa gtaggcgaaa aaatgtcaga agctggatca
    15361 cagttaggca aattcttgaa gaaaacattc caaactgttt cagaaacagt gaatgacaac
    15421 atggaatgga aagatgtcag cttacgtgtc cctgggattg cgacaacgaa atttgagcat
    15481 gaattttact atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac
    15541 gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg aagctaagat caaactttac
    15601 ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac ggagccagat tgaggttaat
    15661 gaagatcata tctccttcca aattccaaat aaacgtgtgc gtgcagacct tgttttctac
    15721 ttacctaaac gagtatacga ccacgcagct attaaacttc taaatggcaa catcatgatt
    15781 gaaacattgg aagctaaaga tatttataca aaatctacga atggtaacat catcgtggac
    15841 caattaactg caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac
    15901 gggaacatct tagattctgt tatcgaaaca gtaaatggta cagtgacatt tggtgcgaca
    15961 gcagaaaatc tttctgtgtc tttagtaaat ggtgatgtac gtcttacgat taaagaagat
    16021 aatttgaaaa aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc tttaccagat
    16081 ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa gtatcaatag ccgtctgtcc
    16141 aattacgaag tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt
    16201 gtttcagatg atgagatcgc gcaagtgcaa ctttcaacga caacaggaag tatctattta
    16261 aaagatacag ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc
    16321 gattttgtaa aatagcttcc cctattttac aaaattgtgc cttattgccg ataggctatc
    16381 tcttgaacac cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga
    16441 acagaggtga gacatgatga aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac
    16501 aggtacttta gcagggattg gggaatatat cgggattgat cccactgtta tccgtgtgct
    16561 ttatgtattc ctaagcttag ttgcttttgg aagtccaatc attttataca ttttattagc
    16621 tttgattatc ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta
    16681 tgaaaaaaac aattatcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
    16741 tgacgaatgg agtgactttt aatgtcgtac tttcagcgta ttttggttaa tacgttaaca
    16801 ttcatatcgt tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacagcc
    16861 gtccttgcca gcttcgtatt atctgtgttg aacatgtttg tcaaaccgat tctgacgatc
    16921 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
    16981 attcttaaaa tgacttcctt ttttgtagga gcagaacatt ttagtttttc ttcatttggt
    17041 gcagcgttat tcgtagctat cttgatgtca cttgtcaatg taatcgttag tgaacataac
    17101 agcagaaagt aaaaaaagct gtgccgagat ctcggcgcag cttttttgtt tatcgcgaat
    17161 tgtaaaatta agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga
    17221 cgaaagaaaa caaaggagtg taaccataaa tgacctacaa acatcttacc atagacgaac
    17281 tgacaatgat agaatcatat tatcttcaac ataataagcc ggttgaaatc gctaaccgaa
    17341 tgggacgtgc tatacaaact atttataatg tagtcaataa gttcaagcaa ggtaagactg
    17401 ctcttgatta ttggcaccag tataaagaaa ataagaaaaa atgtgggaga aaagtcattc
    17461 aattacctgc tcatgaggta gattacatta aagagaaagt cactcttggt tggacgcctg
    17521 acgtcattat cgggcgaaaa gaaagg
//
LOCUS       DAJSVD010000052        17543 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSVD010000052 DAJSVD010000000
VERSION     DAJSVD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.053"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            89..673
                     /locus_tag="ON498_001610"
     CDS             89..673
                     /locus_tag="ON498_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939864.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            684..929
                     /locus_tag="ON498_001611"
     CDS             684..929
                     /locus_tag="ON498_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939865.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            935..1657
                     /locus_tag="ON498_001612"
     CDS             935..1657
                     /locus_tag="ON498_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939866.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1788..2531
                     /locus_tag="ON498_001613"
     CDS             1788..2531
                     /locus_tag="ON498_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939867.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2537..2902
                     /locus_tag="ON498_001614"
     CDS             2537..2902
                     /locus_tag="ON498_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939868.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2918..3448
                     /locus_tag="ON498_001615"
     CDS             2918..3448
                     /locus_tag="ON498_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939869.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3610..3918
                     /locus_tag="ON498_001616"
     CDS             3610..3918
                     /locus_tag="ON498_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939870.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            3914..4333
                     /locus_tag="ON498_001617"
                     /pseudo
     CDS             3914..4333
                     /locus_tag="ON498_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4470..5270
                     /locus_tag="ON498_001618"
     CDS             4470..5270
                     /locus_tag="ON498_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939871.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5307..5489
                     /locus_tag="ON498_001619"
     CDS             5307..5489
                     /locus_tag="ON498_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939872.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5600..5917
                     /locus_tag="ON498_001620"
     CDS             5600..5917
                     /locus_tag="ON498_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939873.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            5920..6152
                     /locus_tag="ON498_001621"
                     /pseudo
     CDS             5920..6152
                     /locus_tag="ON498_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6267..6746)
                     /locus_tag="ON498_001622"
     CDS             complement(6267..6746)
                     /locus_tag="ON498_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939874.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            6866..8203
                     /locus_tag="ON498_001623"
     CDS             6866..8203
                     /locus_tag="ON498_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR3939875.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8226..8753
                     /locus_tag="ON498_001624"
     CDS             8226..8753
                     /locus_tag="ON498_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939876.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            8740..9303
                     /locus_tag="ON498_001625"
     CDS             8740..9303
                     /locus_tag="ON498_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939877.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            9726..10463
                     /locus_tag="ON498_001626"
     CDS             9726..10463
                     /locus_tag="ON498_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939878.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10480..10896)
                     /locus_tag="ON498_001627"
     CDS             complement(10480..10896)
                     /locus_tag="ON498_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939879.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11288..12535
                     /locus_tag="ON498_001628"
     CDS             11288..12535
                     /locus_tag="ON498_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3939880.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            12805..15195
                     /locus_tag="ON498_001629"
     CDS             12805..15195
                     /locus_tag="ON498_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HCR3939881.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15244..17079
                     /locus_tag="ON498_001630"
     CDS             15244..17079
                     /locus_tag="ON498_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3939882.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17148..>17543
                     /locus_tag="ON498_001631"
     CDS             17148..>17543
                     /locus_tag="ON498_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3939883.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGI"
BASE COUNT         6563 a         2444 c         3518 g         5018 t
ORIGIN      
        1 taccataaaa aaaaattaaa aagcgagaca tatttatgtt gttgtgttat cataataaca
       61 taaatatgta gagtgagatg atgaaaaaat ggatttgaaa caatcgtttt acatctggtt
      121 atatcctgat aatcctatgt caataatggc tgatattttt gtagttagca tcttattgta
      181 tttattttat ctaattttct ttcacaattt aatcgggtac atgattcttg attggaaagg
      241 aatgttcgat atagaaagaa aaccatttgc taacaatcaa acctataccg tcttcgaaag
      301 tggagattgg gaagaaaatg aagttgatgt gatccatgaa attggattag ctttatcagg
      361 aatacaagat gctgatgttt tggtatcaat gacaacggat actggataca acgcttatga
      421 ggtattttat cttatgtatt ttatatggcg tgatggtcaa gaaattatca atggtcgtgc
      481 tgaaaaaaca atcaattcta atactgaaat ttctttttgt atgggtgaag gggaaatgat
      541 tgtatataaa gatgggtcag caaacattaa aacagccttt ggtgatattg aaacaagtat
      601 taaggaactt agggaatatg tgcagaaccc atataatttt caatatttag caggaagaat
      661 aaatttagtt taggagtaaa aatatgaaaa aaattataaa tcaaccaaca aatcacttga
      721 taattgaagg tgttattttc gtaacaatgt attttactgt aggaaatggc atctcaaata
      781 caatcattca aaaaaatcca caatacctat tatttagtct ttatgcttgg attgtacaaa
      841 tggtttgggt ttccatattg atgctgttgt tttattggat agtacgggtt attcttttat
      901 tgttaaacaa aagaaaaaag gataggtgaa taaaatgaaa tcaactaaat tgaaggttgt
      961 attgttatcg acagcagcat taataaccct tacagcatgt ggtacaacta atagtgctaa
     1021 taataacaaa gacacaaagt ctagtagtac gatggttact aaaaacagca ctacatcggc
     1081 atcaacaaca atgtcttcaa tgactgaaaa taatgctgaa aagtatcaaa aatacgaaga
     1141 cattaataaa ggtcaaaagc cggatgaagt ttataacaac tttattaata cagtaaaaaa
     1201 tgatgaaaat tatgggaatg atccattgca aaataaaaat ctgactatcg ttctttacaa
     1261 acctgattgt ccagattgtc aaaaagcaga atcaaaaatc atgcaagaaa taaaagaagg
     1321 atttagttat gctccaacat taatgcatga tattaccata aataaaccag cttacgagta
     1381 ttacgcagtt gctgtagata ttactgatgg tgtacctgag tgggtacaaa acattcctgg
     1441 tctggctgat gagaacaatc aatatcatac gccaacagtt gcagtagtaa caccaaagaa
     1501 agttaacggc acactatact gggatgtaat gggaatgaca acaaatacaa ctgatgaaat
     1561 gattgaaagt acaactaaat attatatgga tttgggagca tataaattat atcaacaatc
     1621 agagcctgtt atggacgatc aatccaaagt taaatagatc tttagggcgt aagccctatt
     1681 tttttaaaat taagtgataa caaataaatg atttctgaaa caaacgttct tactcaacta
     1741 ctcgaaacac acaagctaat taactagatt ataaaggaga atgcgtaatg aaagaaatca
     1801 gtgcaaatga agctcatatt ccgcaaaaga atcaggttgg tatatataag gtgcttcatg
     1861 gtgaaggaat ttataaaata cagttactta ccggttacaa taatggtgca aatgggaagt
     1921 ggttaaaggc aacaagtatt cgtgaactta gaagtgaagt agaaaaaatt aggcttggtc
     1981 tcacaagtga agaaatagct aaactgaagg gaaataattc ttttgtaatt gaaaaagaaa
     2041 aggaaaaaca tctactttat cgtgagctga aaattgaaga atactatgaa attgatttac
     2101 ctgaaaaatt aagaattatt gatcaacgtt cagaagaaaa atggaaaact gaaatatatc
     2161 caagcttaaa tgactacaag acatcaggaa agcccatgta taactacaca ggctttttaa
     2221 cctacacaca atacaaatat gcctttcgca aattaaaagg cattaaacaa gctgaaattg
     2281 ccctgtatga gatgaaaaaa gtggatagat tgaaattgat tcaagattta gtgcaaaata
     2341 catggaatca agcgattgtc gagctagaaa aagaaaatgg gaaaatttta actaataaat
     2401 atctgatcgt aaatgaaatt gaagaattaa ttggtttatt ttcatgtgaa aaaggtgaag
     2461 tcttcttgaa gtcatcacca acctattcaa ttttggacag tccgtatcag tttatacagg
     2521 caaggagatg agttagatgt ctagcttgaa agatgactat atccaagcaa aacaagagta
     2581 tgaaaaaata cagatgttga aatctgagaa agtcgaatta tttttaacac aaaaattagc
     2641 agaggatcat attgctaact tagatgctaa aactttagag gaatatgatc ggcagtttaa
     2701 tcagatacat cacaatatca ttttgcttgt tgagcaggca aagcgaaggt ttgaaaaagc
     2761 ggaatatgat ctattagatc aaggaattgt atgcttggat aagggattgc gaagatatta
     2821 tggtgcagag ttaaaaaagc aaaatgtaga tagaaatgca ctaaaacatg aaatcttaga
     2881 attgataatg caacgaattt gaagggatag aaagcaaatg tcacaaaaca ttagtgctgt
     2941 aaaacaatta atagaagaag atttagagac cacagttcag agattagcgg aacgaatcca
     3001 ttttgcaaat tatcaaacca tctctaactg gtcaagcagg aacagaaatc cggaagaaat
     3061 tccaatgaaa tttttttatc gaatcggaaa agaatatggt gttgatccga taaaaatttt
     3121 cgagaagacc atgatttatt actatcatgg aacacctaac gaagtgttag aacatctttt
     3181 ggaagaggca aaagaaaaat catcaatatt agagctatcg caaaatgaca aagttattca
     3241 agtgaaatgc gttgatccaa caggaaagat tattgtcggt tttgatattg aaagaaaatc
     3301 caaaatagat tttattacac aattaaaaaa gataaataat ttagatattc taacgcttag
     3361 accagatcat gaacagttgt ttatgagttt taaaaaacaa ttgaaagaaa tacaaaaaag
     3421 atataacaag gtgtttccta atgcgtgaaa agctcaaaga aatcagagtt ttttggataa
     3481 ttaacaaaga atgtatcatc ttatatactc ttggatttct tagcatgatc ttgttttggt
     3541 ggttatattt aaaacagtaa ttggagaggg tcaagaggat ttttatcaag ataaggatgt
     3601 ggaaactgca tgtggaataa tagattaaaa acaggattgt tattaatagt tatttcctgc
     3661 gctatgatga ttggtatgag gatacaaagg gaacaatcct attttgaagt ttctgctaac
     3721 aacgtaatag aaaaatgcta ttacggtcaa cattattgga gtgaggaagt cagagaaaat
     3781 atagatcgtg aatatgttca aagaattgtt tgggatgcat attctatcaa agattatcct
     3841 aaaagtttaa cgagtagatt attttacagt gaaaaagata atcaaaaact ctcagatttg
     3901 atgatgaaaa aagtatgact attattacga aagagaaaca gagtgctata ttcgagggga
     3961 aaaagcctac aacaaaagtg actattgggg tgacaaggcg aagcgattaa ctgcaaattt
     4021 tagagtgtat gaatcagagt ttataaactt gacctttctt aatagtgttt ttattgatta
     4081 tgtaattact acgaaacgca ttggaagtta ttcaagtacc tacgcatcct tagttccaat
     4141 gttaaactcg atgagccgtt tcttaaaaga aagagaaaag aaagagctac agtatatcaa
     4201 agaaatcatg ccggattttg aaatgcacga atggcaaatt gctttatcag attggaaatt
     4261 ggcgaataag gttcgtagaa taacaccttt tcaagccaag cgctttttga gagctatgaa
     4321 atttgtgcaa taaaaaaagc caccagacaa gttgatggag actaacacaa ttaccaacag
     4381 ttgtaattgt gttagcttac aaattaatta tggtgtttag tcaatcccaa attgtgaaat
     4441 ggcaccaata attggaaagg aaaaatgaaa tgtctgaaga tttacgagag ctgaagttac
     4501 gaaagaagca gtatgatcag tatttttccg gtttgttgaa taaacatatt gaagaaggac
     4561 ttgatcatga ttttggaaaa gatgaatatt gtttatattg cgaacgttac aatgaaaaaa
     4621 tgtctgaata tctacagctg aaaaaagaga tacagaaatt agagaaaaaa gtacatgctt
     4681 ggaagtataa agattccatg tctggttatg ttgaatctca aaataagcca agaaaaaaga
     4741 agcaagcgta tgttctaaag atggtaaatg gggaagagtt aattgtatta gcgtacagtg
     4801 aagaagaggt tcgagaactc gttgaaacac ctgtcaggaa aaaggttgaa acaataaatg
     4861 agatagacga cagccaatac aatagcatct atattgaaac aagtcatggt ggatataaga
     4921 aattagcaac tttccttaga agttctaaag ttgggataat ttctaaaaaa gacataagtg
     4981 ctgttaccga tagtttttgt actttatctg ttcctaaagc ttatttccaa tacgatagta
     5041 aaagatttga acgcttagag aaattctgta gaccgtatac tgtattgaaa actaaaaaat
     5101 ttaataaaac taaaagagtt tctatacagt taccgactgg tcaaatctca ttaggtgaaa
     5161 aagactatct gataaaagta ggaaaagagt taattataaa agtaccggaa cacttattta
     5221 aagaaaattt tgagatacgt gaaaccttgg tatggaaaga agaaaaatag tttggaagac
     5281 atagaatggt ataaggggaa attgaaatgg cagtaaataa aatattgcag gtactaatca
     5341 tagacgaatt tggcggagga gaatggtttg atgtgcagtc catagacttt gaagaagaag
     5401 tcgtagttat agaaaatatt tacggcgaag tatattcata ttcgcttgaa ggtgagaata
     5461 ttgaggattt acggttgatt ggtgagtaga aaaaaacaga aatatcaaaa tggagataaa
     5521 aattaatagc atgtaaagat agtcatgctt gggttaaaaa tgttaaaatt caaaaaaatg
     5581 agtcagaagg gataaaagta tgaaaagaga ggaatacaaa caaagattaa acgaactgct
     5641 tgaagaggac gaaacattaa ctcatggttc gcctgatgaa attctataca tgattgataa
     5701 tatggtgatc tttggtggat atgaacttgg taataggtca gttgatcata atattttaga
     5761 atttgatgat gtatcatggg aagaaattct tgattgggga attttggcgg tacctgaaac
     5821 taaaacctat attagtgaca ctatggttcc tttttttgag gaattggatt ataaaagact
     5881 tcccaaaaat gaaaatcata tactgggagg aaactaacaa tgttaagtga cttatttatt
     5941 ttactgatct atctaggtgt aagtgaaatt attgcgtatt ttttgtttgt cattgctaat
     6001 tatggtattt ccagtgcaat cgttcaaatg tttagattac cagtaagtga actaaaaaaa
     6061 tgatgagatt atcagcatat cttttaatgt ttttgaatgt ggtcacgtta atctttgtag
     6121 taatcggtgt gactttcgat ttaatggaat agtgggaaat ctataatgga aaaaagagac
     6181 acgaatgaac atattttttg aatcaatatt gttgatggtt tttgcgatca gcgcttacca
     6241 gaaatctaaa aaagagtaat gattgtttaa tcatcactct ttttttgtag ggaaattgaa
     6301 atatgtggag cttgtattgg tttagttagg attgcttggc tataaggagc tagttcttta
     6361 tcaaaaccat caaagtaggt tttcagtagc gatttaactg atcgctcatc ttgtttgatt
     6421 tggataattt cagcatccaa atcggttgtg agatcaggag cttttgatgc gattacctga
     6481 ttttttttat cttccaactc tcttaggctg tttaacaagt tgtaaaaaga atcattttga
     6541 ttgttcattc caacgtgagt tgcaatttta acagaagttt cgtccgtatt gtcatcaaaa
     6601 gaactcattg gaatatcaag tagtaacgag gatgtttctt ttactgactt tttctttaca
     6661 gcattttttg tttttccatc ttttttgctc tttactcgag atgtttttgg agctagttta
     6721 ggtaaatcac ttgttttatc agtcatttat tagcttttcc tttctattaa tttatgttat
     6781 tatattatca taataacata aaaaagtaca ctttttgggg gcgtagttat tgaaaaaatc
     6841 taagagaaag attgccacat tgcttatgtt aatggtgcaa tttacaggga ttgcaatgag
     6901 tgtaatcaca ataaatccgc aagtgataca tgctgatggg atagctactt atgatgcaca
     6961 atttaactgt actactgaag gtagtgggtc aggaacagat agcgatggaa acgatactgg
     7021 aagtggcgct gtaacaggtg atgtagccaa aaatattgaa gatatttaca aatacatgaa
     7081 tggagaatat ggcttatcag gtgctagtat tgcaggtatt ttaggtaact ggaaacaaga
     7141 atcaggtatt gatcctcgag cagtagaagg gtattacgga gatggacaga gagaagttga
     7201 cttcatcaaa caatttactg atggtctgat gggtattggc tatggtcaat ggacagctgg
     7261 gcgacataca gcattagttg attggtctaa gaaaaataat ggcggttctt ggtggactac
     7321 agaagcccaa ttggacttta tgttcaaagg tgatggggga ttcgtaggaa tattaaaaga
     7381 ttatgcttta aatgcttctg atgatgtcgt agaaaatgca gtaacttttc ataaaaactg
     7441 ggaaatttca gctgattccg aatcagctgt aatagctgta cgtggtgaaa atgcaaaaga
     7501 aatctggaag tacatgaaaa gcaagggtat ggacggtgca aaagatgaaa gtaagatcaa
     7561 taaaatatcc ggaggttcaa gtaccggaga tggatcgaat aattcatcaa gttcagcatc
     7621 aggaacagta gaagatgcct gtgcaactac ggatgacgct aattctggtt ctggatcaat
     7681 tgggaaagaa ggaaaaatta acggtaaaaa aggtcaagta atagcgaata tgacttatga
     7741 agaagctttg aaaaaatatg ggaaagcgat cacgctacca aaattcaaag aaccagattg
     7801 gtcaacttca ccttttaacg gaatatatca aggtcagtgt acagagttga cgtgggctta
     7861 tatgagccaa ttatacgatg ggaatcaacc gactactgga aatggtggtt atgtatggga
     7921 aagctacaaa tcggcaggag caaaaataac gaacaaacca atcgtaggtt atgggtttag
     7981 tgcttcggat ggatggttat gggctgttgg agatgcaggg catactggtg tagttatagc
     8041 ggtgtttgaa gatggatcat ttctatgtgc taactttaat gttccaccaa cccaagctcc
     8101 aacaagggga gtttcggtaa cgttgattga tggagtggac ggatcagata atgtgcattt
     8161 ctttagcggt gtaggaaaag ctaaatttga aatcgaaaaa taaattggaa tgaaggacga
     8221 tatagatgaa tagtaaagtg aaagatacaa ttatcatttc tttgctttct ggtatgttat
     8281 tagcactgct tgttggaatc ctattcttca ataagcagaa tcaggacata gaagctgaaa
     8341 aacacagtgt gcatattgaa gataagctga cattctttaa agaggttaaa ccaaatgagg
     8401 tggatggatt acggaaagat ttagaaaatg atattcaaga aatgttgaat ggatcagttc
     8461 aaaataagtt tgcagatcaa tcaggtgaag aatttttgag aaaactattt attgtaacag
     8521 gtaaaccacc aattactgat aagagtagtg agaaagaaaa aatcgagtac tataagcctt
     8581 ttacttttaa gctaacaaac tttggaatta agacatctag tagtgggttg tatgaagcat
     8641 tgaccgattt tgaagttcaa tacaatggaa aaaaagtgta tgataccaca ttaaccatca
     8701 cactaaataa tgataagaaa gtgactgggg gtgcttttta tgcaaaatca tgaaatcact
     8761 actgctaaaa aggcgatcgg aatactttcc ataattgtga tcctttgttg tgtggtacta
     8821 tttcaaaaga aaaatgctta tgataataat gtcaaagaat tggaagacct taaaagtaaa
     8881 caaatcgaag tagtcaagaa taaccagaaa gtgaaagcta tgcaggaagc tcaatacaaa
     8941 gaattgggga atgctgaaat caaggaacaa agtaggaaat tctttaattt attttacaac
     9001 tggaattcat gggaaaaata tacgaagaat atgaaggaaa tacaaaaaga atttcctcag
     9061 atagtaaagt ctagtcctgt tgatatttct ggcacgatgg taggcactgg taatagtcca
     9121 atatctagct atactgctga ttactataca acaaatcaaa aaggtaaaat ggttgaattg
     9181 attacacaga caagaaatac tccaacaaca gcaacaacaa caatttggcg tggagtaaca
     9241 agcataggag aaaatcaatt gttttacttg gaggatttag atagctatgt aagagcggag
     9301 taaaataaaa aaagagctaa cggtaccaac gttagctctt tgtgctgacg atgataacaa
     9361 aacctaccga aaaaggattg tttcatcaca aacacaaaca gaaaagaatt gataaaaaac
     9421 ccgaacctca gaatttttat taatcaattc atttagatta taataaattg agctgaacat
     9481 tacaatacgt atatgttgta aaacgtaaaa aaagtcaaaa gtaaatactt tcttaaaaag
     9541 tcaaataaaa ctattaaaga caatatatat ggtttatttg acataagaaa atggtctaga
     9601 tgataattgt aatatagaat tgtaggctta aataaagcat aaaatacctt ttatgataag
     9661 gtatagctct taattttaag cagtttttag atataagagt gaaagtaaag tgggggagaa
     9721 taataatggc agatcgtaaa ttagaaaagt tattagaaga aacgtggaat ccaaaagaat
     9781 ttagtgaatt ttttatggag aattttgaaa ctgacttagc agtgattgtt aaagatgctc
     9841 ttagagagca agggtatcct gaaactgcaa actatataaa cattaatttt actttgtata
     9901 ctgaaaataa aggaacttgg gatttttggg ctactttagc aaataaggag ttaagtgata
     9961 aatcagacac aggaattcgt aacttttttg aatcaaacag agatgattat atgtatgcta
    10021 accatcaaga taaattgaac tttagagtag agtttgatga aacacctgaa gaatttatag
    10081 aaagacaacc gcctaaagaa aatgtagcaa aggtattgga agatcgttgg aattcagatg
    10141 aaatagtttc aacaattagc gaactaggcg gacaatatga accgctagta gaagcggtta
    10201 gagaagagct aagactgaat aagtttcctg atgttcaaaa tattgatgtt tctcaaattg
    10261 aaataaacgt caagatcacg aataaacttg attatggtag ttgggcagat atagcattag
    10321 aaaaatacat ctactcaacg ttaaaagagt ttatcgaaaa tagaatggat atcatgtatc
    10381 tacagcatcc acagtattta aactttggtg ttgaaatagc tacacctctt gaagaatgga
    10441 aaatggagca agggcttgac tagcctttgc tccatttttt tattttacaa atgaatacag
    10501 ttcgtcgttt ttaccaagaa tgattgtttg cccaactttg agattgtcag gtgatccttc
    10561 agcggtttgt aaggagaacg ttccgttgaa cttatcggat accgttatag gtagctttaa
    10621 ttctgatcct tcctcatctt tgagaattgc tgtttttcgt tcatcaaaat aaacagtctt
    10681 tattaaataa tttttcgtag tgatctcagc agatttgtct ttttttgtag tagtaacagt
    10741 ttctttagaa ttagaggtat tttctacgac tattccatct ttggcatttg agcttgtgtt
    10801 tatttgattg aatattttca gcgacaagat accgctaaga atagtcaata tagaaacaag
    10861 tgtaattaat actgataatt tccttttttt gttcattttt tcttaacttc ctttttgttt
    10921 tgattagtta gatattcctt atctaacaag cctttttagg ctattaaatg aagataaacc
    10981 ccaaaaaaat attgcaatta tgacagtgca atggtactat tattatacaa taaattttgc
    11041 gaaaggggta ggtctaaatc gcaaacaaaa agaaattgaa cctagacaaa tacaacttgt
    11101 acaccgcaag ggaagcaagt ttgattttgg gtaaaggtag aaattatgtt acaaacataa
    11161 tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt gacaaaacct
    11221 atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat aaaaacaaaa
    11281 agaaggaatg aatatgaaga aaaattcgct tggtatggtt ttatttgcat cagctgtact
    11341 tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa acacaccgga
    11401 tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag atactatttg
    11461 gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg agtttgccga
    11521 attgaacaat gttgatttgg caaaaggaga acaacggact atcccaattg gacgaacttt
    11581 caacatcagt aaagatacat taacagttaa ggaagcagat ggcgaagtag ttagtcaaac
    11641 tgttcaaaaa gataaacctg atcctgttga aaatacggag caagagaaca aaaaatccga
    11701 gactgcaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa caagtgcttc
    11761 aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga aagaagcatc
    11821 acagggaaca agtgaaagta gcactacgga aactgatact ggaacatcaa caaataactc
    11881 acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg atcaatcaca
    11941 aacaggttcg agtgatgaaa ctgacagttc tacttcacca agtaaacccg aacaaccaac
    12001 taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt gcgaaggaac
    12061 atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt taatgacttt
    12121 tgttaaatca atgccactat ctcctgataa taatccaaat aatggttttt ctggtgaatg
    12181 gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag tttcatgggg
    12241 tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag gattaaaaga
    12301 tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg aacctgctca
    12361 acataaatca atgttttggg gcgatgctat ttggaatcga catgtagtag ccgttgatgg
    12421 atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt ggattaatgc
    12481 aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac gttaataaaa
    12541 gagattgttt atatactaag tagttaagta tagtctttat ttgtccttgc ttaactactt
    12601 agaaaacttg ttgctcatgg tgggtagaac taaaaggggt gatgctatga atactagagc
    12661 gttgaaacag cttgctttca acgaaaaaag tgattaatag gtgtggtgat tttaacttag
    12721 gaatgggcgc gaaggtcatt accacaccga catatcgtgg ctcattttct tcgcctaact
    12781 ggtataaaga aggagtaacg agagatgaag catgaaaaaa cgataatttt aaaatcaaaa
    12841 ataatttatt gttttgcgac tttagtaatg gtgttatcta cgcttataat gcctttttca
    12901 gcaataagag ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac agaaactggc
    12961 gtaaaaacag acggttatat tagggttaat atgccgaatc atccaaatga atggatgatt
    13021 tcaagtcaat ttaaagattc tcatggaaat attggatatt gtatggattc agaattgcca
    13081 tccccaacag gaagtggagc cggaagcttg aagtacaagg gagctggcag tgatgagttc
    13141 tatcgtatgt ttaaaggtgg ttttccatca aaaacagcaa aggaactggg tgcaggaaat
    13201 gatacagaag catggtatgc aacacaatta gtttcatggg ttttagccgg aaactttaaa
    13261 gtttcacaga ttgtatggtc acatccaaac cataccgccg cagaaacagc tcgtgtaaaa
    13321 aaagcatttg aaaaaattta tgactatgct aagaatggaa aagatacgcc caacactgag
    13381 ttttctatca cagcttctaa gacagctgac gaaggaaaat atcacacttt cacatataaa
    13441 acagcatcaa ataagactgg taatgcgaag ctcactttta catcagcaaa accagctggt
    13501 atgaagattt atgatgcaga tggtaaagaa attactaaca acactgtgaa attaaattca
    13561 agttttacta ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt taaaggtact
    13621 gctaatgtta gcacaaccaa tccttttact tttgatggac gaggcgtgta tcaagatgcc
    13681 gtagttatga tcactacttc agaaacaaag gactctaaat ctttgagtgc taaatggaca
    13741 agagctgttg gtcaggtaac agttaagaaa gttgatcaag atggaaatgc tttaaaagga
    13801 gcggagttca ctctaactga caataacaat gctaaatctg ttaagacaac cggagatgat
    13861 gggttagcta cgtttgattt gcaacaaggc ttaacataca gcttagaaga aacaaaagtt
    13921 cctgaaggtt atcaaggttc atttaaaaag gatggtatta ctgttaatac cgatggacaa
    13981 aaatttagtt acactgctac gaacacaatt aaaaaaggta aagtaaaagt taaaaaggtt
    14041 gaccaagatg gaaaaccatt agctaatgct gaatttacct tgacggatga ttcatcccaa
    14101 acacaagcag aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt agcaaacaag
    14161 acctacagcc ttgaagagac cggagtacca actggttata caggcgcatt caagcaagaa
    14221 ggaattaccg ttaaagatga tggacaaact tttgaatata cagctacaaa cactttgaaa
    14281 aaaggtgtag taaaagttca taaagtcgat caagatggaa aacctttggc aaatgcagag
    14341 ttcactttaa ccgatgaaaa aggtgcaaca caagtagaaa aaacagatga aaatggactt
    14401 gcaagcttca atattgtagc aaacaagacg tatagtctag aagaaaccgg agtaccagta
    14461 ggttacacag gtgcatttaa gcaagagggt atcagtgtta aagacgatgg gcaaactttt
    14521 gaatatacag ctacaaataa tttgaaaaaa ggtgtagtaa aagttcataa agtcgatcaa
    14581 gatggaaaac ctttagcaga tgcagaattc accttaacgg atgaaaaagg tgtaaaagaa
    14641 gttaaaaaga cggatgcaac tggtctggct acgttcaaca ttattgcgaa caaaacttac
    14701 aacttagaag aaacaaaagt accaacaggt tacacaggcg cattcaaaca agaaggaatt
    14761 accgtcaaag atgatggaca agtctttgaa tatacagcta aaaacacttt gaaaaaaggt
    14821 gtgatcaaag ttcataaagt cgatgatcaa gggaaagcat tagcaaacgc agaattcact
    14881 ttgacggacg acaaaggtgt aaaagttgta caaaaaacgg acagaaatgg cttggcaaca
    14941 tttaatattg ttgccaacaa aacgtacaaa atcgaagaaa cacaaaaccc taatggttat
    15001 acagggaaat ggtctaaaga aggcatcacc gttgctgacg atggacaaac ctttgaatat
    15061 acagtaatta atcaccttgt acctgaaaca gtaagaacag gcggaatcgg tacatcagcc
    15121 ttccttcttg tatttctaat tgcaggattg agtatggtgc tactgtatta tcgagaacag
    15181 cacaaagcaa aataaaaaag tgttaatgga aaagaagtaa atatgaaaaa agaaggctga
    15241 cgtatgaaat caaaaaaaat tttttcaaat ttaatgctat ttggtacttt gctaggagtg
    15301 ggattgggcg caagctcaat ctcttctagc cttggactag gggggcaagt agcatatgca
    15361 caacaaaact ataattatca aggaactgga acgattcata tccataaagt aacaaatact
    15421 aatggggatg ggaaccctgt ctcagatatt gataacaaag ggaaagaaat agctttacca
    15481 aatggtgtaa aacctcttga aggcgttgag ttctcttatg tgaaacttac taaagatgaa
    15541 gctaaatcct tgggatggac ggatagtgat actacaaaag gtcaacatat tgatgatgct
    15601 aaagcacgat tattactaaa caaaaaatac agtgaagtaa gtagaatgaa gactgattca
    15661 caagggaata tagacatacc tatacaagta aatacagacg atttaacaga taacttgtat
    15721 ttgattgttg aaacaggaac agtcaaaggt gtagttgaag aagcgtatcc tatgattgtt
    15781 aacgtacctg aactaaacgg atcagatgat caatacttta attatgatgt acatgtctat
    15841 cctaaaaact atttccaatt aaactcagca gagcttgata aaaaagacga agatggaaag
    15901 gctcttgccg gaactgtttt tgaattgcga aaagatgatg gatctgatca ggttgttgga
    15961 acttataaaa cagacagcac aggtaaatta actgtggatg gtttggaatt aggtgactat
    16021 tatttcctag aaaccgattt gggaacagat gctaatagaa caacctattt actagatggt
    16081 aaaaaattca aatttagcgt taaacgtggg gagactgcta aagtagttct tgatgttacc
    16141 aactacaaac gacctgtaat tactaaggta attgataaag aaaccgttga tactggcgaa
    16201 gatgttcaat atacattgaa accatcaatt cctggtaata ttggtaatta cgaaaaatat
    16261 gttgtcaaag atgttctagg tgcagagtta acttatgatt catcagaagt gatcgaagga
    16321 atgactttag atactgatta cagtatcgaa cgtgacggac aaacgacaaa ctatgtcttt
    16381 actgaatcag gtatggataa gcttcagaaa caatttgatg caggggatcg cttctttaca
    16441 attttgatga acgctcatac taatagtaaa gctaaaatgg atacaccact taagaatgat
    16501 gtttcaacga tctacaacaa tggatatgaa cccgaagacg aaacaccagt tgatcacgct
    16561 tatacaatca ttggtgatca ccaattcatt aaaacagacg gagatacaag caaagcattg
    16621 agtggagcaa aatttgtagt gaagaacaaa attgatccga ataaagttac ttacctaaaa
    16681 caagatccaa caacgaaagc tgttacttgg attgcagata aatctaaagc tactgaattc
    16741 gtttccggat cagacggtaa gttccaaatt gaaggtttgc aatataccta tgatgtagat
    16801 tttgataaac aaggtaatcc aacaatcaca gataaaaaaa tcaatgatta tgctcttgaa
    16861 gaagttgttg ctccttcagg atatacacca actagaaacg atgttccatt tgtggtatcg
    16921 aatgatgatg cttctagcga agtaacagac atcaagaacc ttaaaacacc tgaaacagtt
    16981 cgtacaggtg gaattggaac agtagtattt ctagtggttg caggtgtggc aggaacaggg
    17041 ctatatgttg tctacaaacg agaacaacac aaagcttaat gaaataaaat tccctagagt
    17101 gtttttggct ctagggtttc tttttaaaaa gtgtgtgaaa agtaggtatg agcatgaaaa
    17161 agaagaatat caagttgatc atcttaaaaa ttgggatgtt gctatgttta ggagtaggtc
    17221 tatatcctgt aattactcag gcttatacct actaccaaac tcaacaaaca ctaaactcct
    17281 ataaggaaaa atatgatgaa gcttctgaaa aggagaatga agaagtcttc aagagatatt
    17341 cagaagttaa taggaaacga aaagacacag aacaaacctc agtcacgatt cctgaagata
    17401 ataacaatat tccgtcaaat ggaaatcttc agaaggataa attaggtgaa ttagtgggtg
    17461 aaattcttat accaaagatt aatgtggatt tgccaattta tgatggtgta aacgatttcc
    17521 aactatcagc aggagcagga ata
//
LOCUS       DAJSVD010000053        17272 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSVD010000053 DAJSVD010000000
VERSION     DAJSVD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.054"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            71..418
                     /locus_tag="ON498_001632"
     CDS             71..418
                     /locus_tag="ON498_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939884.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
     gene            513..1397
                     /locus_tag="ON498_001633"
     CDS             513..1397
                     /locus_tag="ON498_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3939885.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1621..4788
                     /locus_tag="ON498_001634"
     CDS             1621..4788
                     /locus_tag="ON498_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939886.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4808..5353
                     /locus_tag="ON498_001635"
     CDS             4808..5353
                     /locus_tag="ON498_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCR3939887.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5381..5836
                     /locus_tag="ON498_001636"
     CDS             5381..5836
                     /locus_tag="ON498_001636"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCR3939888.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5833..6591
                     /locus_tag="ON498_001637"
     CDS             5833..6591
                     /locus_tag="ON498_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3939889.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6677..7417)
                     /locus_tag="ON498_001638"
     CDS             complement(6677..7417)
                     /locus_tag="ON498_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3939890.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7478..8731
                     /locus_tag="ON498_001639"
     CDS             7478..8731
                     /locus_tag="ON498_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3939891.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9053..9280
                     /locus_tag="ON498_001640"
     CDS             9053..9280
                     /locus_tag="ON498_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3939892.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9403..9729
                     /locus_tag="ON498_001641"
     CDS             9403..9729
                     /locus_tag="ON498_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3939893.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9797..10510
                     /locus_tag="ON498_001642"
     CDS             9797..10510
                     /locus_tag="ON498_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3939894.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10820..11671
                     /locus_tag="ON498_001643"
     CDS             10820..11671
                     /locus_tag="ON498_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3939895.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11792..13657
                     /locus_tag="ON498_001644"
     CDS             11792..13657
                     /locus_tag="ON498_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3939896.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13705..15114
                     /locus_tag="ON498_001645"
     CDS             13705..15114
                     /locus_tag="ON498_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3939897.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15222..15923
                     /locus_tag="ON498_001646"
     CDS             15222..15923
                     /locus_tag="ON498_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3939898.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16082..16561
                     /locus_tag="ON498_001647"
                     /pseudo
     CDS             16082..16561
                     /locus_tag="ON498_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5760 a         2904 c         3713 g         4895 t
ORIGIN      
        1 tcttttattt ctagaagtta ttcacgtata cggatttgtt tttctcacaa ataaaaaggc
       61 aggaagtaca atggatcatt tgtatataaa atatattcat caattttccg ttttggtgtt
      121 aggcacacta attatagcgg ggattgtcgc atttattatt caaggccgtg acatattaac
      181 agtacatcaa gagtttttca gttatgcatt tggtattcct ctgatcgcta taaggtctga
      241 taacaaaaag tggaagaaac gctattacat tctcttgcta tttttgtgtt tttatctgcc
      301 acctaagatt tttactttat taggtataca atgcctatca ttttctttta tttcctatat
      361 cattgtttta attttcctca gtttcatagt atggatgttt tatcttgtca aaaaataaat
      421 gaatgtttca tgaacagtac ttcatcacag cctaattttt ttctttatgc tatcgttata
      481 ttaaagaaat caatcagaag ggaaagaaag tgatgaaaaa agttttatta gtggtaaacc
      541 ccagttcagg cggtgaacaa gcaaaggaat ttgagcaatt agcgatagca aaattggaat
      601 cggtgtttga tgaagtggtc gtcttgcata caaaaaaagc aggggatgca aaaaacttta
      661 ctcgcgaagc ggctacggaa gggtatcata gtgtgtttgt catgggcgga gatggaacag
      721 tcaacgaagg aatcagtgga atcgctgaac aagaacatcg gccaaatttc ggatttttcc
      781 cattgggtac ggtgaatgac ttggctcgag cattagggat accgctagaa cctgaggaag
      841 cgatcaatca tttttcgatt gaatcagtca agccattgga tattggaaaa ataaatgacg
      901 actattttat gaatgtcgtc gcaattggct ctatccctga atcaatcaat gatgtagatg
      961 cggagaagaa gacgaaattt ggaaaactag cttatttcat gtcaggaatc aaacagttag
     1021 cttccacaca atcctactcg tttcacgtag aagttgatgg aaagaaggaa gaaattgaga
     1081 gcagtacctt gctgatagga ctaactaact cagtcggcgg ttttgaaaca ttacttccaa
     1141 atgcaaaagt ggatgatggt aagctccatt tggtttattt aaaagattct tctttgatcg
     1201 atacgcttaa aacattgcca gacctattaa aaggagtaga tgaatctacg aaaaatctag
     1261 cctaccttac ttgtgagcaa ttagttgtgt cattagctga tgaggaagaa ttgacgacga
     1321 atgttgatgg agatgaaggg gataaattac ctgtgacaat caaagtattg ccctcacatt
     1381 tacaggttta ttgttaaaat ggaggttgtg accgaggagt tggcgggaga acaccgcttg
     1441 ttcgggataa gggactgtga caagactttt gtcacagcct tttttttgta caaaaaagaa
     1501 atttacacct aatagaaaaa tttttccata aaaatacata ttttaaatat gaaagtacaa
     1561 ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga
     1621 atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg
     1681 gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa
     1741 agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa
     1801 agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     1861 gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg
     1921 tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact
     1981 aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc
     2041 gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag
     2101 atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg
     2161 acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc
     2221 agatatcatt ttacgggaga aataaaattt accgggacac ttgatttgta tgatgggtcc
     2281 aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg
     2341 ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt
     2401 agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa
     2461 ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc
     2521 catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat
     2581 gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga
     2641 agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc
     2701 caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat
     2761 acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt
     2821 ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg
     2881 cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc
     2941 gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca
     3001 gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac
     3061 agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3121 ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat
     3181 tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa
     3241 acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat
     3301 gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga
     3361 agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag
     3421 atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa
     3481 aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc
     3541 aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3601 atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga
     3661 gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3721 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca
     3781 gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat
     3841 gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa
     3901 gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa
     3961 gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4021 cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca
     4081 ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa
     4141 acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca
     4201 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg
     4261 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     4321 gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc
     4381 tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa
     4441 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg
     4501 aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt
     4561 tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa
     4621 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat
     4681 ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     4741 tactcagcag agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg
     4801 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat
     4861 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     4921 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     4981 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     5041 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     5101 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     5161 aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa
     5221 agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa
     5281 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     5341 aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta
     5401 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     5461 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     5521 cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     5581 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     5641 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     5701 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     5761 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     5821 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     5881 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     5941 tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     6001 ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta
     6061 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     6121 acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag
     6181 aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa
     6241 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     6301 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt
     6361 tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact
     6421 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     6481 gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt
     6541 tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc
     6601 aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg
     6661 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     6721 tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat
     6781 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta
     6841 aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt
     6901 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     6961 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     7021 cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     7081 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     7141 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     7201 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     7261 aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag
     7321 tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt
     7381 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     7441 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     7501 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     7561 agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc
     7621 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     7681 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     7741 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     7801 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     7861 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     7921 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     7981 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     8041 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     8101 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     8161 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     8221 aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     8281 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     8341 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     8401 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     8461 tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc
     8521 gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     8581 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     8641 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     8701 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa
     8761 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     8821 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     8881 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     8941 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     9001 tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac
     9061 ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt
     9121 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     9181 agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga
     9241 cacgctactg catacacatg gtggctacct aaattattga ttttctatga catttatttt
     9301 aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg
     9361 tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa
     9421 gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct
     9481 atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa
     9541 gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat
     9601 tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg
     9661 atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca
     9721 gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag
     9781 tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct
     9841 cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta
     9901 ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa
     9961 aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga
    10021 aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt
    10081 cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta
    10141 cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag
    10201 ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc
    10261 caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa
    10321 agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact
    10381 aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag
    10441 tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa
    10501 aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa
    10561 tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat
    10621 ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat gaaaaggctt
    10681 gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg ttactgtacg
    10741 gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt ttttattttt
    10801 cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg tcgtcatttc
    10861 gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa
    10921 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgttttaa gagattcttt
    10981 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
    11041 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
    11101 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
    11161 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
    11221 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
    11281 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
    11341 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
    11401 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
    11461 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
    11521 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
    11581 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
    11641 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
    11701 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
    11761 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
    11821 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    11881 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    11941 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    12001 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    12061 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    12121 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    12181 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgcag cagatggtgt
    12241 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    12301 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    12361 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    12421 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    12481 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    12541 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    12601 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    12661 aagtttatgg caagtattcg tgatgttcgg catgcattgg ggattcgtac cgatcatgtt
    12721 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    12781 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    12841 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    12901 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    12961 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    13021 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    13081 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    13141 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggagaac cgattacttc
    13201 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    13261 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    13321 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    13381 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    13441 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    13501 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    13561 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    13621 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    13681 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    13741 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    13801 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    13861 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    13921 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    13981 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    14041 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    14101 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    14161 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    14221 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    14281 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    14341 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    14401 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    14461 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    14521 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    14581 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    14641 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    14701 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    14761 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    14821 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    14881 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    14941 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    15001 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    15061 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    15121 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    15181 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    15241 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    15301 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    15361 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    15421 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    15481 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    15541 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    15601 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    15661 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    15721 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    15781 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    15841 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    15901 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    15961 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    16021 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    16081 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    16141 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    16201 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    16261 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    16321 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    16381 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    16441 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    16501 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    16561 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    16621 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    16681 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    16741 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    16801 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    16861 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    16921 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    16981 cagctattta tcgttaacaa aaagattttt aggagaagct gatgaacaaa aatgatcaaa
    17041 ttttgatcgt ttagttcgtc ggctttttta tttagagaaa catcatttat cagtgaaaca
    17101 taacacgaga acaaaatgat tttttcgtta ataaaagaaa caagtatttg ttgtttaaaa
    17161 taatacacgt tattggcata taggacgaaa tcccagtatc ttctataagt aagtcatttc
    17221 atatcgatga aaacacgtta tttatttttg ttttatctat aaaatggctg aa
//
LOCUS       DAJSVD010000054        16839 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSVD010000054 DAJSVD010000000
VERSION     DAJSVD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.055"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            162..1103
                     /locus_tag="ON498_001648"
     CDS             162..1103
                     /locus_tag="ON498_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3939899.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1172..1903
                     /locus_tag="ON498_001649"
     CDS             1172..1903
                     /locus_tag="ON498_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR3939900.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2047..4227
                     /locus_tag="ON498_001650"
     CDS             2047..4227
                     /locus_tag="ON498_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3939901.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4247..4729
                     /locus_tag="ON498_001651"
     CDS             4247..4729
                     /locus_tag="ON498_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3939902.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4745..5506
                     /locus_tag="ON498_001652"
     CDS             4745..5506
                     /locus_tag="ON498_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3939903.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5637..6971
                     /locus_tag="ON498_001653"
     CDS             5637..6971
                     /locus_tag="ON498_001653"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3939904.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7278..7763
                     /locus_tag="ON498_001654"
     CDS             7278..7763
                     /locus_tag="ON498_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR3939905.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7756..8439
                     /locus_tag="ON498_001655"
     CDS             7756..8439
                     /locus_tag="ON498_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3939906.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8565..11210
                     /gene="polA"
                     /locus_tag="ON498_001656"
     CDS             8565..11210
                     /gene="polA"
                     /locus_tag="ON498_001656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3939907.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11255..12091
                     /gene="mutM"
                     /locus_tag="ON498_001657"
     CDS             11255..12091
                     /gene="mutM"
                     /locus_tag="ON498_001657"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR3939908.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQYIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12055..12684
                     /locus_tag="ON498_001658"
     CDS             12055..12684
                     /locus_tag="ON498_001658"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3939909.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12736..13068
                     /locus_tag="ON498_001659"
     CDS             12736..13068
                     /locus_tag="ON498_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3939910.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13380..13871
                     /gene="nrdR"
                     /locus_tag="ON498_001660"
     CDS             13380..13871
                     /gene="nrdR"
                     /locus_tag="ON498_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR3939911.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13894..15267
                     /locus_tag="ON498_001661"
     CDS             13894..15267
                     /locus_tag="ON498_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3939912.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15267..16193
                     /gene="dnaI"
                     /locus_tag="ON498_001662"
     CDS             15267..16193
                     /gene="dnaI"
                     /locus_tag="ON498_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR3939913.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16322..16762
                     /locus_tag="ON498_001663"
     CDS             16322..16762
                     /locus_tag="ON498_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3939914.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5750 a         2947 c         3709 g         4433 t
ORIGIN      
        1 ttttctgtgc gaaacaggaa agcgaagtct ttttaattca aataataata ttatgtaaac
       61 aaaaaacttt ccatgcgtaa ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc
      121 tttatactgt ttaggaacga aaagaaggaa ggaagaaata catggattat caagtactgc
      181 tttattacaa ttatacaaaa atcgaagaac cagaggaatt tgccaaaaat catttagcct
      241 tttgcaaatc tttggatctt aaaggtcgta ttctagtagc tgaagaagga ataaatggta
      301 ctgtgtctgg aaccgttgaa aacacgactg cctatatgaa agcaatgaaa gctgatcctc
      361 gatttgctga tacgatgttc aagatcgatc caagcgaagg acatgccttt aagaagatgt
      421 ttgtaagata ccgtccagag cttgtaagtt taaagctaga agaggacatt gatccaaacc
      481 aagtgacagg tgcctatctt tcaccgcaag aatttaaaga agctatttta gatgaacaga
      541 cagtggtgat tgatgcacgg aatgattatg aatatgactt gggacatttt cgaggggctg
      601 tacgcccaga tatacgcagt tttcgagagc ttccagactg gatccgcgag cacaaagaag
      661 aatttatgga aaaacgggta gtgacctact gtactggtgg cattcgttgc gagaagtttt
      721 ctggttggct tgtccgtgaa ggattcaaag atgtcggaca gctgcatggc ggtattgcta
      781 cttatggtaa ggatcctgaa gtccaaggag aattatggga cggcaagatg tatgtgtttg
      841 atgaacggat cgctgtcgat atcaaccaag tagataaaca aatcgtcggt cgcgactggt
      901 ttgacggaac accatgtgaa cgatatatca attgtagcaa tcctgagtgc aatcgtcaga
      961 ttcttgcttc cattgaaaat gaagaaaaac atttaggagc atgtagcata gagtgtgcga
     1021 aacatccgcg caatcgctat gttcaagacc atggaatctc tccagaagaa gcagaactgc
     1081 ggtttacagc tttaattaaa tagaaaacaa tagttttgat tgtcgaccat ttccaatatg
     1141 cgctatacta gcgttgagga tggtgatcca catgaaatca ttgatttatg ctattgggga
     1201 tgtccatggc aaacatgagc tttttgaaaa acttattcac tattatgatc caaagatcca
     1261 tcagcttgtt ttattagggg atctcaatga tcgaggtcct cgtacaaaag aatgtttttt
     1321 attaggaaag caattagtcg aagaaacagg agccatttac ctccggggaa accatgaaga
     1381 atattttttg caatttctgc aatcaccgga ggactggtat gcgccttata tgagaaatgg
     1441 tggaaaagaa acgtttgaaa gcctgattca cccaggggca tcggaggaat actccccaac
     1501 tgaaatctcg atgatgatcc aatcgagata ttcagaactg attaaatttt tagtccagcg
     1561 ccctttgtat tatgaatggg aaaattatct ttttgtccat gccggtgtgg atttgacaaa
     1621 gaaagactgg cgtaagacaa gtctaagaga ctttatttgg ataagggaac catttcatga
     1681 agggaaaaac aatacaggta agacaattgt atttggacat acgattacac caatgctcca
     1741 tggagatatg cagacaacag acctctggat tcaggatcat aaaatcggaa tggacggtgg
     1801 ggctgtattc ggagggtcat tgcatggggt tgtttttgac aaggaaggta ttcttcaaga
     1861 tattgaatat cagaacaacg aaggaccttg gcatggaatc tgactaatgg aaagaatgaa
     1921 ggagctgtga ctttgttgcg gctcttttta tttttataga gaatattgtt tctttcagta
     1981 gtttaaccca ggataaaaag tatgatagaa taaagaagca atttttagca gaaaagaggc
     2041 attttaatga cggaaaattt gaaccagacg atcattcagc ttgtacaaaa agaactttca
     2101 gactatcgtc ctaagcagtt gactactgta cttaatttgt taaatgaagg aaacactgtt
     2161 ccttttatcg cgcgttaccg aaaagaaatg actggaagtc ttgatgaagt ccagattcgt
     2221 gaaatcgaag agcggtatgc ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt
     2281 cggctaatcg atgaacaagg gaaactgact cccgagttag aagcagaaat cacccaagcc
     2341 gtgaaaatgc aacaagtgga agatttgtat cgtccataca aacaaaaacg acggacaaaa
     2401 gcaacgattg ctaaagaaaa aggacttgaa ccactcgcga agtggctgat gcagcttaca
     2461 gatggagagg tgcaatctga agcagaaaaa tatattgaca aagaaaaaga agtctcttct
     2521 gccgaagaag cgttgcacgg tgctcatgag attattgctg aacaagtcag cgataatgca
     2581 aaatttcgga cttggattcg gtcttatacg tataacaaag gtatgtatgt cagcaatgtc
     2641 aaggatgagc aagcagatga aaaaggcgta tatgaaatgt attatgactt tgcagaaccc
     2701 gtccataaaa tggtttctca tcgaatatta gctactaatc gtggagaaaa agaagatatt
     2761 ctgaaagtat tctttcaagt agatgaagca gcaattttag cttacttgga tcgccaaata
     2821 gtcaaaaatc ctgcctctcc ttcttcttca ttcgtcagag aagcctatca ggatagttat
     2881 aaacgcttta ttcagccagc aatcgagcga gaactgcgca atgaattgac tgaaaaggca
     2941 gatgagcaag caatcgctat tttcggcgaa aacctccgta atcttttgtt gcaaccgcca
     3001 ctaaaaggta aagttgtcct gggatttgat ccggcttatc gtactggatg taaactagca
     3061 gtcgtagatg cgacagggaa agttttagca atcgaagtta tttatcctca taaacctgca
     3121 gcgcaagcaa aaagagaagc tgctggacca gcttttatcc aattaattaa taaatatcaa
     3181 gtagatatgg tagctatcgg aaatggtaca gccagtcggg aatctgaact ttttgtggct
     3241 gagcagttga aatccgccga ccataaaacc tattacgcta tcgtcaacga agcaggcgct
     3301 tctgtttact ccgctagcga aattgcacga aaagaatttc cgcatctaca agttgaagaa
     3361 agaagtgctg tttctattgc ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc
     3421 gatcctaaag ctgtcggagt aggccagtac caacatgacg tttctcaaaa acgtttggct
     3481 gaacaattgg attttgttgt ggaaacagcg gttaaccaag taggtgtgga tgtgaataca
     3541 gcaagcccgc agctcttgca gcatatatca ggattgaata aaacaactgc ccaaaacatt
     3601 gtcatttatc gtgaagaaaa cggagaattc accgcacgaa ctcaattgaa aaaagtgcca
     3661 cgcttaggac caaaagcata tgaacaagca atcggattct tgcgagtccc tggtgggaaa
     3721 aacattttgg acaacacagg tatccatccc gaaagttatt ctattgcaaa agaaatatta
     3781 acttcagtcc aactttcaga aaaagagctt ggtacagaag aagcagtaga aaaattgaca
     3841 cgattatccg tagagaaact ggctgaaagc ctatcagtcg gagaagaaac gttagccgat
     3901 attttagctg ggctgacaca acctggacga gatatgcgtg atgagatgcc agcgccctta
     3961 ttacgtacag atgtattgag tatggaagac ttgaaaccag ggatggaatt gactggaacg
     4021 gtccgtaatg tcattgactt cggcgcattt gttgatatcg gtgtgaagca agacggactt
     4081 gtccatatat cgaaactcag caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta
     4141 ggagatatcg taacagtttg gatagaacaa gtcgatacga aaaaaggcag aatcagtttg
     4201 acgatgcttt ccccttatga agagtaacaa tgaggagtaa caaaaattga cacaagaaga
     4261 actgcagcag ttagtagaag agatatcatt aaaaagcttc cagcttcctt tcaagcacca
     4321 agcaatgttc aatccacgct tacgtacaac tggcgggcgc tatcatttaa acgatcatca
     4381 tttagatttt aatgcgaagc tatttgaaga gtcagacgaa gagacgattg caggaatcat
     4441 caaacatgaa ctttgccatt atcatcttca tctagctgga agagggtatc agcataaaga
     4501 tatggatttc aagtatcttt tgaaaaaaac aggagggttg cgctatgcac cagccttgac
     4561 gaagaaacag ataaagatgg agtattatcg atgccaagat tgttctgcca cgatctgccg
     4621 caagagaaga atcgatgtac gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg
     4681 gattgagaca aaaagagaag actttaatga agcacaaaaa atcagatgaa aggatagaag
     4741 gcctttggaa aaaagtgagc agaggaatct aaaagactgg aatcagctat taaatttttt
     4801 agccgcacct gaagagggaa attttgagga actgacggca cctacactta ttttagctgg
     4861 aaactgtctt cctatcttag ctgataaagc agcacaaatg tatttaaatg gccaagtaga
     4921 tcaattgttt ttggtcggag gggtggggca tgcgactaga atcctttatg agaattttga
     4981 aaagcaagga tttcattttg aagatggcat gagtgaaagt gaaatctgtc gtcagtatct
     5041 aaaagaagtc tatgatttgc ccgataaggc ttttctgata gaatcgaagt ctacaaattc
     5101 tggagagaac gccacttttt cactggagat tcttcattcc ttaggagctg taccagaaaa
     5161 aatcttattg atgaatgatc cgacactgca acgcaggact cgagcaactt ttgagaaagt
     5221 atggcaaaat gaacaaacta ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca
     5281 cttttctgaa gagatcatct ttacagcaaa agaactgaat catcaatggc ctaaggaata
     5341 tttccatgcg ctagtattag gagaaatgga acgtctccat gacgatgaga acggctatgg
     5401 tccgaaagga aaagatttta ttcctcacat tgatatatca gaggaagttt ggtcttctta
     5461 caccagaatt aaagagtcaa taaaaacgga tttttctcgt acttgaccat tttagcaagc
     5521 gaatcctcct ttccaaataa atttggttga aattttcaat cgtttataag ataatgagga
     5581 gcgtgttgaa tgtaatagct aaaagaatgg acgctagata agagaagagg attcaaatgg
     5641 aaaatcaaaa taaattgtat cattttgttg gtatcaaagg ttcaggaatg agctccttgg
     5701 ctcttgtttt acatgaacaa ggtctaaatg tccaaggatc agacattgaa aaatatttct
     5761 ttacacaaag agatttagaa aaagcaaata tcacgattct gccatttaac gcagataatg
     5821 taaaaccagg gatgacgatc attgcaggaa atgcatttcc tgattcacat gaagaaatcc
     5881 agcgagcaaa agaattaggt ttggaagtga ttcgctatca cgactttatt ggtcatttta
     5941 tccaaaatta tacgagtatc gctgtaacag gctctcacgg aaaaacaagt acgactggac
     6001 tgctctctca tgtattgagt ggtgtccgtc caacaagtta tctaattgga gatggaacag
     6061 gacatggtga tccgcaagcg gaattctttt catttgaggc ctgtgaatat cgccgtcatt
     6121 tccttgccta ttcaccagac tatgcaatca tgaccaatat cgattttgat catccggatt
     6181 attatacaag tatcgacgat gtatttacag ctttccaaac aatggctgga caagtgaaaa
     6241 aagctatttt tgcgtatggt gacgatgctt atttacgcaa attaaaagca aatgtgccga
     6301 tttattatta tggtgtcaca gaaaacgatg atatccaagc acgaaacatc gaacgcacaa
     6361 cttctggttc tgcttttgat gtctatcatg gggatgaatt tgttgggcac ttcactgtac
     6421 cagcattcgg taaacacaat atcttaaatg ctttaggtgt tattgcagta gcttatttcg
     6481 aaaaactgga tctgaaagaa gttgcagaag aaatgctgac tttcccagga gtaaaacgtc
     6541 gattcagtga aaagatcgta gcagacatga ccgtagtcga tgattatgcg catcaccctg
     6601 cagagatcaa agcaacgatt gatggcgcac gccaaaaata tccagataaa gaaataattg
     6661 cagttttcca accccatacg tttacgcgta cgattgcatt gatggatgaa tttgcagaag
     6721 cacttgattt agctgataaa gtttacttat gcgatatttt tggttcggct cgggaagaac
     6781 aaggaaacgt taagattgaa gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca
     6841 aagaaaataa tgtatctcca ttgttagatt atcatgatgc agtagtaatc ttcatgggtg
     6901 ctggagatgt tcaaaaattt gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa
     6961 atgtactgta atacaattac tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa
     7021 aaatcttgcc aagccccctt ttttgcaact tatgataaac ggtgttcaaa agcttgcatc
     7081 tgcggtaata agcccaatca aatcaaaaac atgagaagct gttttttctt ggctgttctt
     7141 attatttgat gcaggacagc tttttcacga cctctgtaaa ctataggaat ctgttaaatc
     7201 aagggagcag aacttgcctt ttacgctact tttggtacaa taatgtatga ttaaaaataa
     7261 actggagggt ttctatcttg aacataggta agccgagaaa actaggaaaa acaataaatg
     7321 aaatcgagga aggcgactcg ttatccttaa cagaatctat tgaagataac cagcttttgc
     7381 tgtatttggg attgacgaat gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa
     7441 aaacagaata tcaacagcca atcgttccat cagtgatgct gatggggatc atcacaagtg
     7501 caatttcaaa gcatcttcct ggaccaggat cacatgttgt caatttttct gtcaatttta
     7561 ttgaaccggt tttccattat gaaacactga cttttcagtt tgaggtcata aaagtagata
     7621 agatgaagga tgtggtgacg atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt
     7681 tggatgctgt cgttatggtc caaccgccac aagttcctga attggaaata gcaatcgaaa
     7741 gtgaaggtga attgaatgaa taatccaagt acatcaatgt caggtatcaa tcgtttttat
     7801 gcaaaagttt atggattttt aggtatggga cttgccctta gtgcagtcac ttcttacttg
     7861 gctctttggg tatttccttt gcaattagca acttttatca ataattttcc actaggcttt
     7921 gtgggtctgt ggatcgttga actgatttta gtaatggttc taggcgtaaa agcacaaaag
     7981 aatccgagct tagcaatcgg tggatttatt gcctactctc ttttgaatgg cataacatta
     8041 gcggttactc ttgcatacta cgatattgga acagtaacgc aagctttctt gacagctact
     8101 ttgatgttca tggggatgtc tgttgtcgga gtaacaacaa aaagagatct tagcggggtt
     8161 ggaagagcag gacaggcagc tttaatcgga gtgatcattg cactagtctt gaacatcttc
     8221 attttgagaa gtagcgtagt tgagctgttc atctccctac tgttagtcgt catctttgca
     8281 gggattacag cttatgataa ccaaagcatc cgtaatatct atgtccagac aaacggaaca
     8341 gcacatagtg gaattgctgt attcatggca ttgaagctgt atttggactt tatcaacttg
     8401 ttcttggcgt ttctgagaat ctttggacgg aacaactaaa aaagaaaaaa attcatcagt
     8461 tggggctata ccgaattatt tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt
     8521 ctttgttaga atgaataaga actttacagt aaggagttaa gaagatgaca aaaaataaat
     8581 tattactcgt tgatgggaac agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc
     8641 ttgaacgttt caaaaataga aacggtcttc atacaaatgc aatctatgca ttcaatacga
     8701 tgtttgaaaa tgttatgcaa aaagagcaac cgactcatgt tctggttgcg ttcgatgcag
     8761 gaaaaacgac ttttcggaca gaaatgtatg cggagtacaa aggtggaaga tccaaaacac
     8821 ctggcgaatt caaagaacaa atgccatata tacgtgattt gttgacagga ctaggggtac
     8881 agtattacga attgcccaat tatgaagcag atgatattat cggtactttg gcagaaaaag
     8941 tagacaaaga tcaatttgat gtcgtcgtgc tttcaggtga tcgggatttg actcaattag
     9001 ctagtaaaga agtaaaagtg gatatcactg taaaaggtgt cagcgatatc gaaagctata
     9061 caccagaaca tgtagcggaa aaatatgatg ggttgacgcc aaaacagatt attgacatga
     9121 agggactagc cggagatgcc tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa
     9181 cagctatcaa gctattgaaa caatatggct cagtcgaagg aatttatgaa catatcgatg
     9241 aaatgaagca aagcaaaatg aaagaaaatc tgataaatga caaagagcag gctttcttat
     9301 cgaaaaaatt ggcgaccatc aatacttcag cgcctgtgga tgtctctatc gactcattga
     9361 agtatgaagg gaaaaatctc gataaactcg ttccgtttta taaagaaatg gatttcaatc
     9421 agttcctttc taaattgaat atcgtagaag aagaagtaaa aatggatgat attttgtttg
     9481 aagtcgttca tgaattcaaa gaggaaatgt ttactacgga tatggcgttg tacgtggaaa
     9541 tgatgggcga caattatcat acagaggaaa tcgtcggagt agcgtggggg acagaaaaga
     9601 aaatttatgt cacgaacgat ctatctgtat tcgaaaaccg agcgttccat tcttggatca
     9661 ctgatccgac tcggctgaaa aaagtatatg atgcgaaacg aacttatgtt gctttaaacc
     9721 gctatactgg aaaaacgaaa ggaatcgatt ttgatgtctt gctaggtgca taccttctcg
     9781 atacgaatga aaaaagcaca gatatcgaag gaatagctgc tcattacgga tacaacgata
     9841 tccaatcaga tgaagctgta tacggcaaag gggcgaaaaa aggtttacca gaagaagagg
     9901 aactcttttt tgctcattta gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat
     9961 tgagccaaga actggcagat aaaaaccaag aagatctttt ccggaaaatg gaactgccat
    10021 tgtctatcat tttggcagag atggaaataa gcgggatcaa agtagatgcg actcgcttac
    10081 aagaaatgaa gggcgagttc tctgctcgct taagagagat cgaacaaaaa atctacgaag
    10141 aagccggcga agaattcaat ttgaattcac caaagcaatt aggagtgatc ttatttgaaa
    10201 aaatgggatt acccgttatc aaaaaaacaa aaactggata ttccacagcc gtagatgtac
    10261 tggaacagct tcgtgaacaa gcaccaatcg tagaggatat cctcacctat cgtcaaattg
    10321 caaaaattca atctacctat gtcgagggac tgctgaaagt gatccaatca gatggaaaag
    10381 tgcatacccg ttatgtccag acattgaccc aaacaggacg attaagctct gttgatccta
    10441 atttgcagaa catcccgatt cgattagatg aagggagaaa gatacgtcag gcatttgttc
    10501 ctagagaaaa agactggctg atttattctt ctgactattc acaaatcgag ttacgcgtac
    10561 ttgcgcatat ctcaaatgat gaacatttga aagctgcctt cctagaaggt caagatatcc
    10621 atgccagcac agcgatgcgt gtatttggaa tcgaaaaggc agaggatgtc acaccaaata
    10681 tgcgtaggca ggcaaaagca gtgaattttg gtatcgtgta tggcatctct gattacggac
    10741 tttcacaaaa tctaggaatc agccgaaaag cagcacagca atacatcgat acgtactttg
    10801 aaaaataccc aggtgtcaaa gaatatatgg aaagaatcgt acgcgaagca aaagaccaag
    10861 gctacgtcga aacgctgtat caccgccgtc gatatctgcc agacatcaat tcaagaaatt
    10921 acaatatccg ttcttttgcg gaacgaacag ctatcaatac accaatccaa ggaagtgctg
    10981 ctgatatttt gaaaatcgcg atgatcgaat tggataaacg attaaaagaa acaggtcttc
    11041 aagctacgat gctgttacaa gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac
    11101 ttgaatcatt agataaattg gtgaaggaag ttatggaaca agctgtttct cttcatgtac
    11161 cactgatcac tgacagcagt tggggaaaaa cgtggtacga agcaaaataa acattgttta
    11221 agacagagca atataatgaa ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac
    11281 cgttcgtaaa ggattggaac ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt
    11341 gtggcccaag atcatcgagc aaccagagac acccatcttt gaggcttctc ttgttggcga
    11401 aaccatccaa tacattggac gtagagggaa gtttttaatt tttcatctcg atcattgtga
    11461 actgatctca cacttgcgaa tggaaggaaa atatcaattt acgaaagaaa atacaccaat
    11521 cgataaacat acgcatgtac ttttcttttt tgaggatgga agccaacttc gctacaacga
    11581 tgtacgaaaa tttggtcgta tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg
    11641 aatcatgaaa ttgggacctg agcctttgcc agatagcttc ttattagcag atttcgcaaa
    11701 tggactgaaa aagtcccaca aagcaatcaa gccattgctg ctagaccaac gtctagtgac
    11761 tggtctaggt aatatttatg tagatgaagc attatgggaa gcgaagatcc atccagaaca
    11821 accggcgaac acactgcgcc ccaaagaggt agaacaatta cgtctatcga tcattgatgt
    11881 tttggatcgt gcaatagaag ccggaggtac cacgattcgc agttatttga atgcattagg
    11941 agaatcaggc ggatttcaag tagcactgca tgtctatcag caaactggca aaccatgtat
    12001 acgttgtgga acgccgatcg tgaaaacaaa agtggcacaa agggggacgc actattgtcc
    12061 gaattgtcaa aggctaaaaa tcgcaaagta ggatttgttt taggtttgac gggtggaata
    12121 gctaccggta aaagtacggc agccaaagtc tttcagtctc atggatttcc tttgatcgat
    12181 ggcgatgtga ttgccagaga aactgttgag cctggaacgc ttgctctgag gaaaattgta
    12241 tcagtgtttg gccaagaggt attgcagcct gatgggcaac tagatcgagg gaaacttggg
    12301 atgattgttt ttccatcaaa ggaaaaacgg caaaaacttg atcagctatt agatccgttt
    12361 atccgaaaag cgataaaaga tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt
    12421 gtagatatcc cattgctcta cgaaggtcat tatgaccact atatggatgc agtagctgtc
    12481 gtttatacta cgccagaaac tcagcttcag cgtttgatga aacgtaatca actgacgcta
    12541 gaacaagcgc agcagagaat tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg
    12601 gatattttgt tcgacaacaa tggaacaaaa gaaaaacttg tgggacaaat cgaaaattgg
    12661 ctttttgcta atcgctttct ttgagtatct gtgtcaattt tgctatcctg agtatataga
    12721 aagtgaggga tagcgatggg atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca
    12781 aaagaaaaaa tcggcgagat gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa
    12841 tcagaaacag cagaagaaaa agaaaagcag taccaagcaa gaatcgatga aatagaagac
    12901 cagcaggcag aagaagaagc gaagcggcaa gatttgactc aagaacgaaa aaatccgaca
    12961 cctgatcgta gtaaaacacc gctcccagaa agtgaccaca tttccgatga gcaaaaaatc
    13021 gaggaacagc gaatgaaaca ctatacagga atcgaagaga agttataaaa tagtaaaaaa
    13081 tcgagatgtg acttgaagca gatgagaagg tgttcatctg cttcaagttt tttgtgctag
    13141 atgactaaat cagcttttgt gtaagttatt tctcagtaat caaggtgatc agtattggaa
    13201 aattgaaatt tagtaaggac aggggacaca attacgcatg aaagtagaaa aaattgttct
    13261 tatatccgtg ttttttgggt aggacagtcg cgtttgtgat ttgtttcgtg ttataataca
    13321 actagttgta aagaaagaca catcctttat ataaaatgcg atgcaagagg tgagacaaaa
    13381 tgagatgtcc cagatgtcaa cataataatt cacgagtaat cgacagccgt caagcagatg
    13441 atggacgtgc gatccgtcgc cgtcgcgaat gtgagaattg ttcgttccga ttcactacat
    13501 ttgaacgaat tgaagctgct ccgttattag taatcaagaa aaacggggaa cgcgaagagt
    13561 tcaatcgcga taaaatttta agaggactca tccgttcagc agaaaaaaga cctgtagcta
    13621 tggaacaaat ggaacaaatc gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa
    13681 acgaagtatc gacgaatcta attggtgaat atgtgatgga agatttagtt gatttagacg
    13741 aaatcgctta tattcgtttt gcaagtgttt atcgccagtt caaagatatg tctgttttct
    13801 taaaagagtt gcaagatatc gtggataaag caaaagaaaa taataaagag aataataaag
    13861 aaaacaatta agaaacaaaa aggaggcatt tccttggaaa atgcttggaa agagcttcaa
    13921 ccaaaaaata tttaccaagg aagaaaagcg gctccgctga gagaagacag caagaacgcc
    13981 ttgctgtatc tttatcagcc actaatcggc ggagaagcct tgagtatcta tttcaccctg
    14041 ttatcagaaa tctctcaaga atctggcaaa ggtcctgaag ggatgcatgc agatctgctt
    14101 agtagtctag gctgcggtat cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc
    14161 ggcttactgg atgtttattt taaagaagat agtgatctgg gacctcgttt tatttatgag
    14221 ctgttagaac cactcattcc taaagcattt ttcaaagact ctgttctggc ttttcttctt
    14281 ctggaaaaag ttggtgaacg acgattccag aagcttaccc aattctttga acctaaaagg
    14341 catacagcag agggatatca aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat
    14401 gaagcagtgt acgctgctaa tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa
    14461 tctgaatcag tagcattact tgaagcagat tcgacattcg attggcagtt tttgaatgat
    14521 tggctggcaa aaaaacatat tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg
    14581 tatcatcagc tttacggttt cgatgaattg acacttggcg aactgatcag tcaatcttat
    14641 gactttagtg aacaaaaagt atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag
    14701 agacgtccgg tgaagcaaac aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc
    14761 tcgtcaaacg aacatttgac aaacgcagcg cttcagctga taaaagaagc acagagcatt
    14821 ccaccgatga aatatctgga agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa
    14881 gaaaactggt tactgcaaga tcttgtttct caatcaggtc tttccagtag cgtgatcaat
    14941 gttttgctga attatgtact agtgatcaag aataatgcta gcttaaatgc cagttatgta
    15001 aatacagtgg caaatgaatg ggcacaaaag aaaatcgcca cagcagaaga agcgatccag
    15061 cacattcggc aaataagtaa tcaagcaaaa caatcaaaac aaaagaagca aacaaattat
    15121 caaactggca agcgaaatgt tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa
    15181 gatgaaaaac agatcagcag cgagaaaaaa gcagagattg accgccgttt taaagaatac
    15241 ctggcaaaaa aggagggtga tatctagtgg aagacgtagg caaagaattg aagaggatca
    15301 tcagcaaacg caattatcaa gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg
    15361 cggatgttca agcttttcta agcgcacacc aagaagaatt gacacaagcg gatattgaaa
    15421 aaagttacgc taaattgtat gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc
    15481 caacgatgat cgctcccggc tatgagccga agctgacgtt gaattttcat tatgtggatg
    15541 tcacttatgt accaacggaa gcattgttag cacggcaaag acaagaagaa atcagaagcc
    15601 gagtaaaagc aatggatatg ccaaaagata ttcaatcggc ttcttttgaa aactttgaac
    15661 gtacagatgg acgaggatat gcgagcttag aagccctaga ttttgtagag aaatatactg
    15721 ctgatccaaa agggtatcat aaaggactgt atctagtagg aagttttggt attggaaaaa
    15781 catatttatt aggagcaaca gcgagagaat tagcgattgc tggattcacc acgacgctag
    15841 tccattttcc gacatttgcc gttgagatga aacaggcaat cggaaaagat caagtggcag
    15901 aaaagttaga tgcagtgaaa cgttcaccta ttttgatgat cgatgacatt ggtgcagatg
    15961 cgatgagtag ttggatacga gacgaagtat tcggtgttat ccttcaatat cggatgcagg
    16021 aacagttacc aaccttcttc tcatcgaatt ttacaatgga tgaattggaa cagcatttgt
    16081 ccgtcactca acgtggagat gaagagccgc taaaagcgaa acggataatg gaacgcatac
    16141 gttatttgac aaaagaaatc gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg
    16201 acaagtccaa ctgacatttg acctagtagt aaaagcaggt cagcgaatca ttatatggac
    16261 agaaatgacc ggagacaatc accgcaacaa gtaatgcaga aacttaaacg gaggattttt
    16321 tatgagggct attttttcta gtatatctgt agttggtcta ttttttcttt tagcaggctg
    16381 cggtagcgca gatagcaaca caggttctgt tgattcgatt gatgcgggtc aaacagtaaa
    16441 aacaagcagt acgaaaacgg agagacaaag catatttgtc ggaacgattg aaagtattac
    16501 tcaagtagaa gcagaagaag aaacgcttca gttgaaactc gtcgatgtca tggccaaaga
    16561 agatccagcg aatatgggaa cgtcattcag caatgacgga gtgatcttaa atgctactgc
    16621 tgaaaaaata agcgtggaca taaatgaatt gaaaatcggt gacaaagtcc aatttacatt
    16681 agttgagatg ccgatcatga cgatgtctat cccaccgcaa gttcctggag attcgattat
    16741 tgatgtttca ttgaacaaat aaccaataac attttttcaa aaataagaaa atgttgtttt
    16801 atagaacgaa aaggtatatt ttttaaggag atatgggaa
//
LOCUS       DAJSVD010000055        16815 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSVD010000055 DAJSVD010000000
VERSION     DAJSVD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.056"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..456)
                     /locus_tag="ON498_001664"
     CDS             complement(<1..456)
                     /locus_tag="ON498_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3939915.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="ON498_001665"
     CDS             complement(561..1424)
                     /locus_tag="ON498_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3939916.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLAEEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="ON498_001666"
     CDS             complement(1620..3719)
                     /locus_tag="ON498_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3939917.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLSEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="ON498_001667"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="ON498_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3939918.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="ON498_001668"
     CDS             complement(5072..6913)
                     /locus_tag="ON498_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3939919.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="ON498_001669"
     CDS             complement(7095..9410)
                     /locus_tag="ON498_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3939920.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="ON498_001670"
     CDS             complement(9595..10455)
                     /locus_tag="ON498_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR3939921.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="ON498_001671"
     CDS             complement(10514..11035)
                     /locus_tag="ON498_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR3939922.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="ON498_001672"
     CDS             11313..12074
                     /locus_tag="ON498_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3939923.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="ON498_001673"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="ON498_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3939924.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="ON498_001674"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="ON498_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3939925.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="ON498_001675"
     CDS             16434..16790
                     /locus_tag="ON498_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3939926.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4613 a         3492 c         2945 g         5765 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttcc
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccgacag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gcataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttaga
//
LOCUS       DAJSVD010000056        16502 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSVD010000056 DAJSVD010000000
VERSION     DAJSVD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.057"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(28..726)
                     /locus_tag="ON498_001676"
     CDS             complement(28..726)
                     /locus_tag="ON498_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR3939927.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="ON498_001677"
     CDS             complement(719..2071)
                     /locus_tag="ON498_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR3939928.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="ON498_001678"
     CDS             complement(2294..3694)
                     /locus_tag="ON498_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR3939929.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="ON498_001679"
     CDS             3855..4592
                     /locus_tag="ON498_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR3939930.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="ON498_001680"
     CDS             4717..5196
                     /locus_tag="ON498_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR3939931.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="ON498_001681"
     CDS             5193..5960
                     /locus_tag="ON498_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939932.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="ON498_001682"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="ON498_001682"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3939933.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="ON498_001683"
     CDS             complement(6863..7465)
                     /locus_tag="ON498_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3939934.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="ON498_001684"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="ON498_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="ON498_001685"
     CDS             8146..8730
                     /locus_tag="ON498_001685"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3939935.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="ON498_001686"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="ON498_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3939936.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="ON498_001687"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="ON498_001687"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3939937.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="ON498_001688"
     CDS             10631..10810
                     /locus_tag="ON498_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939938.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="ON498_001689"
     CDS             10832..11857
                     /locus_tag="ON498_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3939939.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="ON498_001690"
     CDS             12032..13276
                     /locus_tag="ON498_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3939940.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="ON498_001691"
     CDS             13429..14376
                     /locus_tag="ON498_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3939941.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="ON498_001692"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="ON498_001692"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3939942.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="ON498_001693"
     CDS             complement(15278..15520)
                     /locus_tag="ON498_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939943.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="ON498_001694"
     tRNA            15641..15713
                     /locus_tag="ON498_001694"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="ON498_001695"
     CDS             complement(15832..16470)
                     /locus_tag="ON498_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3939944.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5625 a         2947 c         3318 g         4612 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 ga
//
LOCUS       DAJSVD010000057        16408 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSVD010000057 DAJSVD010000000
VERSION     DAJSVD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.058"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..1835)
                     /locus_tag="ON498_001696"
     CDS             complement(<1..1835)
                     /locus_tag="ON498_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3939945.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ON498_001697"
     CDS             complement(1832..2536)
                     /locus_tag="ON498_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3939946.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ON498_001698"
     CDS             complement(2548..4857)
                     /locus_tag="ON498_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3939947.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ON498_001699"
     CDS             complement(5052..5516)
                     /locus_tag="ON498_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939948.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ON498_001700"
     CDS             complement(5516..6142)
                     /locus_tag="ON498_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3939949.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ON498_001701"
     CDS             complement(6149..6514)
                     /locus_tag="ON498_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR3939950.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ON498_001702"
     CDS             complement(6504..6842)
                     /locus_tag="ON498_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939951.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ON498_001703"
     CDS             complement(6832..7158)
                     /locus_tag="ON498_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3939952.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ON498_001704"
     CDS             complement(7136..7417)
                     /locus_tag="ON498_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR3939953.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ON498_001705"
     CDS             complement(7419..8783)
                     /locus_tag="ON498_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3939954.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ON498_001706"
     CDS             complement(8796..9365)
                     /locus_tag="ON498_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR3939955.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ON498_001707"
     CDS             complement(9337..10524)
                     /locus_tag="ON498_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3939956.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ON498_001708"
     CDS             complement(10588..12315)
                     /locus_tag="ON498_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3939957.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ON498_001709"
     CDS             complement(12312..12764)
                     /locus_tag="ON498_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR3939958.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ON498_001710"
     CDS             complement(12876..13256)
                     /locus_tag="ON498_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3939959.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ON498_001711"
     CDS             complement(13253..13639)
                     /locus_tag="ON498_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939960.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ON498_001712"
     CDS             complement(13641..13919)
                     /locus_tag="ON498_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939961.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ON498_001713"
     CDS             13967..14695
                     /locus_tag="ON498_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR3939962.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ON498_001714"
     CDS             complement(14841..15317)
                     /locus_tag="ON498_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HCR3939963.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15624)
                     /locus_tag="ON498_001715"
     CDS             complement(15277..15624)
                     /locus_tag="ON498_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939964.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ON498_001716"
     CDS             complement(15611..15796)
                     /locus_tag="ON498_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939965.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ON498_001717"
     CDS             complement(15797..16069)
                     /locus_tag="ON498_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HCR3939966.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16269)
                     /locus_tag="ON498_001718"
     CDS             complement(16066..16269)
                     /locus_tag="ON498_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939967.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT         4606 a         3462 c         2546 g         5794 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcatc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattatcttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcagcttttt tatttgatat aatacttgta tcgattaatc aaatcgaaat taaatttaaa
    16381 aaggtgattt atcatgaata tcaacaat
//
LOCUS       DAJSVD010000058        16232 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSVD010000058 DAJSVD010000000
VERSION     DAJSVD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.059"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            226..894
                     /locus_tag="ON498_001719"
     CDS             226..894
                     /locus_tag="ON498_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3939968.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="ON498_001720"
     CDS             complement(1094..1324)
                     /locus_tag="ON498_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939969.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="ON498_001721"
     CDS             complement(1451..2833)
                     /locus_tag="ON498_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3939970.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ON498_001722"
     CDS             complement(2989..4176)
                     /locus_tag="ON498_001722"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3939971.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ON498_001723"
     CDS             complement(4183..5214)
                     /locus_tag="ON498_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3939972.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ON498_001724"
     CDS             complement(5230..6684)
                     /locus_tag="ON498_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3939973.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="ON498_001725"
     CDS             6859..7764
                     /locus_tag="ON498_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3939974.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ON498_001726"
     CDS             7855..8532
                     /locus_tag="ON498_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3939975.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ON498_001727"
     CDS             8529..8699
                     /locus_tag="ON498_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939976.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ON498_001728"
     CDS             8813..9709
                     /locus_tag="ON498_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3939977.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ON498_001729"
     CDS             complement(9764..11134)
                     /locus_tag="ON498_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3939978.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ON498_001730"
     CDS             11372..12118
                     /locus_tag="ON498_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3939979.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ON498_001731"
     CDS             12115..12954
                     /locus_tag="ON498_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3939980.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ON498_001732"
     CDS             12955..13905
                     /locus_tag="ON498_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3939981.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ON498_001733"
     CDS             14046..14798
                     /locus_tag="ON498_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3939982.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ON498_001734"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ON498_001734"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3939983.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5274 a         3019 c         3251 g         4688 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catagaaaaa cgattacttt gcttatacaa caaggctttt atggttttgt
    16201 atcaaaaaaa tatttgttta aatgttacaa at
//
LOCUS       DAJSVD010000059        16002 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSVD010000059 DAJSVD010000000
VERSION     DAJSVD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.060"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            293..979
                     /locus_tag="ON498_001735"
     CDS             293..979
                     /locus_tag="ON498_001735"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3939984.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            999..4580
                     /gene="smc"
                     /locus_tag="ON498_001736"
     CDS             999..4580
                     /gene="smc"
                     /locus_tag="ON498_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR3939985.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4589..5398
                     /locus_tag="ON498_001737"
     CDS             4589..5398
                     /locus_tag="ON498_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3939986.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5409..6407
                     /gene="ftsY"
                     /locus_tag="ON498_001738"
     CDS             5409..6407
                     /gene="ftsY"
                     /locus_tag="ON498_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3939987.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6594..8861
                     /gene="gshAB"
                     /locus_tag="ON498_001739"
     CDS             6594..8861
                     /gene="gshAB"
                     /locus_tag="ON498_001739"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR3939988.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(8950..9402)
                     /locus_tag="ON498_001740"
     CDS             complement(8950..9402)
                     /locus_tag="ON498_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR3939989.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            9552..10502
                     /locus_tag="ON498_001741"
     CDS             9552..10502
                     /locus_tag="ON498_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3939990.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10495..11454
                     /locus_tag="ON498_001742"
     CDS             10495..11454
                     /locus_tag="ON498_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3939991.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            11451..12206
                     /locus_tag="ON498_001743"
     CDS             11451..12206
                     /locus_tag="ON498_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3939992.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            12229..13182
                     /locus_tag="ON498_001744"
     CDS             12229..13182
                     /locus_tag="ON498_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3939993.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(13826..14122)
                     /locus_tag="ON498_001745"
     CDS             complement(13826..14122)
                     /locus_tag="ON498_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR3939994.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            14477..14833
                     /locus_tag="ON498_001746"
     CDS             14477..14833
                     /locus_tag="ON498_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3939995.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            14843..15742
                     /locus_tag="ON498_001747"
     CDS             14843..15742
                     /locus_tag="ON498_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR3939996.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         5331 a         2776 c         3381 g         4514 t
ORIGIN      
        1 ctgtttattt ctctaatatt caggtcaagg cgctgatttc atgacttctc caataaacgg
       61 tgttcaaagt ccagtttctc ggtattaagt caaatttatg caaaaatatg gaaagccatt
      121 ttcacaaaat tttttcttca tactcgaaac taaacgaatt tttcacaact tctcgtcaat
      181 cactaggatt attaaagctt tacttacctt ttgaccgaac catttcaaat ttgacaaaaa
      241 accagtataa taaggaagta tcgaaaaaat atccataaaa ggaatgtgga aaatggacaa
      301 ccagctgaca aaagaactaa aagaaaacta taacatcgtt tttcacgatc taaatttgct
      361 tgaacaagct tttacccatt catcctatgt gaatgagcat cgcaatctgc aattatcgga
      421 taatgagcga cttgaatttt taggagatgc cgttttagaa ctgatggttt ctcagtattt
      481 attccgttta tatcctcatt tgccagaagg aaaattgaca aaaacacgag ctgcgatcgt
      541 tcgcgaagac agcttgtcga aatttgccaa agaatgtcac tttgatcaat atgttttgtt
      601 gggcaaggga gaagagaatt caggaggacg tacgcgacca gcattgcttt gcgacctatt
      661 cgaagctttt ttaggcgcgt tatatcttga ccagggattc gacacgaccc atgcattcat
      721 cgaaaaagtg atttttccta aagtcaaagc aggtgctttt tcacatgaga tggatcataa
      781 aacaaaacta caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat accgattgat
      841 caatgaagaa ggtccagctc atgaacgagt attttggatc gaagtttatg tagatgatca
      901 attgattggc actggacaag ggaaatcgaa aaaattagca gaacaagctg cggcggaaaa
      961 tgcattggca gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa cgaattgaaa
     1021 tagccggttt taaatcattt gcagacaaga cagtcattga ttttgaaaat agcgtcacag
     1081 cagtggttgg accaaatggc agcggaaaaa gcaatattac agaggctatc cgctgggtat
     1141 taggagaaca atctgctaaa agtctgcgag gcggaaaaat gccggatatc atctttgccg
     1201 gttccgatac ccgtaagcag ctgaatattg cagaagtcac agtgatttta gacaatagtg
     1261 atcattatct gccattggag tacaatgaga tcagtgtaac ccgccgttat cgacggactg
     1321 gggaaagcga gtttttcatc aataaacaaa gctgcagatt aaaagatatc caagaattgt
     1381 tcttagattc tggattagga aaagaatcgt tctcaatcat ttctcaagga aaagtagaag
     1441 cgattttcag tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca gctggtgtct
     1501 tgaaatacaa gcagcggaaa aagaaagcag aacagaagct ttttgaaaca gaagacaacc
     1561 tcagccgcgt ccaagacatc atccacgaat tggaagaaca gttaactcct ttggccgccc
     1621 agagtgaggc agcaaaggag tttttacggc taaaagaaac actgactcaa accgatgtct
     1681 ctttgatggt tgctgaaatc aagacagcaa aaaaagattg ggacaacaaa caagcacagc
     1741 ttgcgaaatt caatctagaa ttaggcaaac tatctgagtc gatccaagaa caagaatcca
     1801 ttttggcgaa gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa aaaaatcagc
     1861 aagtattatt agatctttcg gaaaaactga agcagacaga aggacaaaaa gatgttttgc
     1921 aggagcggac aaagcataca caaaaaagct cgcaagaata tcaaacttca ttagcagaag
     1981 cacaaaaaaa ggttaaacat tttgaaaagc ttcaagaaag cttgatgaaa gcagcagcag
     2041 aaaaagaaac agagatccaa aaagctgaag caaatctcat aaaaacacag caagaattag
     2101 aaaaatacca aaaatcaacg aaagaattat tggcagaatt aagagatcaa tatgttgatt
     2161 tgatgcaaga acaagcagct gtaggaaacg aactgaagta tctagaacgt caatatatac
     2221 aagaaacagc aaaaagcaag cagacgttag ccaagcaatc ggaagtagaa gcatcagtag
     2281 accgattgat cttacaaaaa gaagaattga cacaaaagca ggcgcagcta aaatcatctt
     2341 taactgaaac aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag ttccaagaag
     2401 cattagcaaa agaacagcct aaaatgtatc aattaatgaa tcaagtccag cagctacgtg
     2461 ctcgtaagaa aagccttcaa gaaatccaag aaaactattt tggtttttac caaggggttc
     2521 gattggtctt gcagcataaa cagcagcttt cgggtattgt cggagcagtg gctgaactga
     2581 tcgatgttcc tgcagatttc acgttagcta tcgaagcagc tttaggtggg gcagcccagc
     2641 atgtcatcgt tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg aaacagcaaa
     2701 gaggaggaag agcaacattc cttccactga cgacaatcaa accaagacaa ttaccggctc
     2761 atatattgac acaagcagca gctgtagaag ggtttatagg gattgccagt gaacaagtca
     2821 gttatcccga tcaaatccaa actgtggttc aaaacctgct aggaacgatc cttttagcca
     2881 aagacttaac tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat cgagtggttt
     2941 ccttagaagg tgatgtgatg aatgccggag gatccatgac aggtggagcg aataaacgag
     3001 gaaaccaagg cagtttgttt gtacaaaatc aagaattgaa acagttgact agtgaatttg
     3061 aagaagcaga taaacaactc caagcacaag aaaaaaaagt tcaggagctg caacaagaaa
     3121 cagctcgttt agccgaagaa caagaagttt tgcgtacacg aggcgaacaa ttacgttttg
     3181 aagaacaaga agcgacaaat cagctacaaa acatcataaa tgaacttgaa cgttttgaaa
     3241 aagaaaagca aatctcaact tttgagacac gtgaactgca gcagtttatc gaagattacg
     3301 aaaagcaaac gaacgaattg aaagacaaac aaacagatct tgaatcgcaa cgacaaaaaa
     3361 tcgacgaaga aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc agaagagcac
     3421 aagtacaaag tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa gagcagttta
     3481 atcatctgca gatccagctc cgcggtgccc gagtccaaaa agcagaggca accgaacgtc
     3541 aagaagcgat agaaaagcag ttggcgacat tgacagcaga cttttctgat catgaagtca
     3601 cagaagaaag tttggaaaaa caaattaacg aattatctgc tcagcgagaa acattaaaag
     3661 ccgagttggc aaaagccaaa gagcagcgag atagaaggca aaaggagatc gataagcttg
     3721 aagcagtctt ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa caatccaaat
     3781 tagaagtcca aaaagaccga gctgaaatga ttttagataa tcatttgacc tatctgcagt
     3841 cagaatatca gctgacattt gagaaagctt cccaagacta tcaagaaaca accgatatcg
     3901 aagacagtag aacaaaggtt tcatccttaa aagaacagat tgaaaaactg ggaccagtaa
     3961 acttaaattc aatcgaacaa tacgaacaag tcagtgaaag acatacattc ttagcaacac
     4021 agcgagatga tctgttagca gcgaaaaacc aattgtttga aacgatggat gagatggatg
     4081 atgaagtgcg gacaagattc aaagaagtat ttgaagctat ccgtcaagaa ttcaaaattg
     4141 tctttccaaa tatgtttggc ggcggtaaag ctgaattagt actgacggaa ccaactgatt
     4201 tgctgaaaac aggtattgag attgaagtac aacctcctgg caaaaaactg caaagtctaa
     4261 gtttgctttc tggaggagaa cgagcactta cagccatcgc gttgctcttt tccattatcc
     4321 gtgtctgtcc tgttccgttt tgtatcttag atgaggtaga agcagcgtta gatgaagcca
     4381 acgtgaaacg ttttggtcgt tacttgagcg atttccaaga tgacacacaa tttatcgtag
     4441 tcacacaccg caaaggaacg atggtcgcag cagatgtact gtacggagtg acgatgcagg
     4501 aatccggtgt atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa gaggcgaaaa
     4561 tcaacgaaac gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg atttggacgg
     4621 tacattatta aatgaacaaa agctgatttc agatgagaat aaacaagcat tagcacaagc
     4681 aaaacagcaa ggggtcaaga tcgttctatg tacaggacgc cccttagctg cgatggcgca
     4741 ttatctgcaa gaattaggac tggtagatga aggtgactac agcatcactt tcaacggcgg
     4801 tttggtacaa aaaaatgata ctggtgaaat catggaaaag aaagtgatgg aagtatcaga
     4861 tatccaacgc ttatacaaac tggctcagca attggatctg ccactagatg ttttgtctga
     4921 caatgtggtt ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca atgtgttgaa
     4981 taagctgctg caattccaac cagcggatat aagcgatatt acagatgaat ttgtgcttaa
     5041 taaggcagta atcgcttatg accaagaaga attagatcct aaaatcaaag aaataccagc
     5101 agaattccat gaaatgtatg agatcatcaa aacaagaagt atgcttttgg aatttatgcc
     5161 aaaaggtgtt acaaaagctt atggtatttc tttattggca aaagatcttg gtttgaaaca
     5221 agaagaaatc atggcgatcg gtgatgagga aaatgattta ccgatgatcc aatatgctgg
     5281 actaggcgta gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag atttcgtgac
     5341 agccagcaat gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat tagagtaggg
     5401 ggagtcaaat gggatttttt gataaaatca agcgtgcttt tactggcgaa gagaaaacag
     5461 aagaagttga agtacaggag aagtacgaca agggtttaga aaagacgcgt aaaacatttg
     5521 gtgaacggat gaatgagttg tttgccaatt ttcgaacagt cgatgaagac ttttttgaag
     5581 aattagaaga aacacttatc ggtgcagatg taggttttga aacagcgata cgaatcaccg
     5641 aagcattacg tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg
     5701 cgatcatcga gaaacttgtg gatctctatg aagcggaagg aatcaatgaa gtaaatgaac
     5761 taaatatcca agaagacgga ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga
     5821 aaacaacaag tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga
     5881 tggcagcagc agataccttc cgcgctggag caatcgacca gttagttgtc tggggcgaac
     5941 gcgctggtgt tgaagttgtt cgtggcaatg caggaggcga tccagcagct gtcgtattcg
     6001 atgctttgga aagagcaaag acagagcagg cagatgtttt actagttgat actgcaggcc
     6061 gactgcaaaa taaagtgaac ttgatgaacg aattagaaaa aataaaacga gtaatccagc
     6121 gggaattacc agaagcaccc caagaaacac ttcttgtatt agatgcaacc actggtcaaa
     6181 acgcgatggt tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt cttgttttga
     6241 caaaattaga tggtacagca aagggcggta tcgtacttgc tatccgtaat gaattgcatt
     6301 taccagtgaa attggtcggt cttggtgaag gaatcgatga cttggagccg tttgatccaa
     6361 atgattttgt cgttggattg ttcaaaggat tactaaaaga agaataaccc atgtctaaaa
     6421 agcaggagct gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt aacatagctg
     6481 tgtttttgat gccttcattt ttatacttat ttgtaagcgg ttctcaaaca atttcgttct
     6541 ttttcttaga ctttttgatt ataattagat tcatatgaat tggaggtcac ttaatgatga
     6601 attttaagca attattattg catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg
     6661 gtatagaacg ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc
     6721 cagcaatctt tggcgatcga tcctatcacc cctatatcca aacagatttt agcgaaacac
     6781 aaacagagat gatcacccct gttaccgatt ctattcccga attatttcag tatctcgctg
     6841 ctgtttatga tgtgactgct cgttctatac cgaaagaaga gatgatctgg ccattaagta
     6901 tgccacctgc cttaccggaa aaagacgaag agatcattat tgcaaaatta aaaaatttcg
     6961 aagatgtctt gtatcgacgt tatttagcaa aagaatacgg gaagcgaaaa cagatggtaa
     7021 gcggtattca tttcaatttt gaatttggcg atgagttgct aagaacattg ttcagccatc
     7081 aggaagaatt tcaagatttt tccgaattca aaacagaact ttatttgaaa acagcgagaa
     7141 actttatgcg ctatcgctgg atgatcactt atttattcgg tgcttcacca atgagtgaga
     7201 agaactactt tttagatgaa tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg
     7261 cgctgggcta tacgaatcat ccaaatgtga aagtttccta tgcttctatg aaacagtatt
     7321 tagcagatat cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa gaattttata
     7381 caccgcttcg tttccgaggc ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt
     7441 atattgaatt gcggaatatc gatttgaatc cttatgcaag attgggtatc aatccagagc
     7501 aagttcggtt cttacaatta ttcctgatgt acatgttatg gacagaagaa aaagaagact
     7561 gtgaccaatg ggtagcagaa ggtacgactc gaaataacaa agtagcacta gaacaaccat
     7621 cagatcaaac agaatttcat caagaaggta gagaaattct tgaaggaatg aagcaaatgc
     7681 tggttgaatt ggattggttg gattctcttt atttagtaga ggaagcattg actcaaatgg
     7741 atcatcctga acaaacgtta gcagcgaaac tctatcaaga agcacagcta tcgagtcagc
     7801 aagaagttgc tgtggcattg ggacatcaat attataaaga aagccatgaa cgtccctatc
     7861 agttggccgg ttttcgcgaa atggaacttt ctactcagat atttatgttc gatgccatcc
     7921 aaaaaggtgt ccaagtcaaa gtattagatg agtcggatca atttttgcgt ctgcaattcc
     7981 aagaccatgt agaatatgtg aaaaatgcaa acatgacaag taaggacagc tatatcgtcc
     8041 cacttatcat ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct gggttccggg
     8101 taccaggagg cgcagaattt tcatccatgg aagaagcggt aaaagcttat ccgagatttg
     8161 ccgagcaggc atttgtgatc aaacctaaat caaccaatta cgggttaggt atcacgattt
     8221 tcaaggaagg agctagtctg gaagattatc aagcaggttt agccattgct tttcgtgaag
     8281 acagttcggt tttagtagaa gaatttatgc cgggaacgga atatcgtttc tttgtgatcg
     8341 acggagaagt ccaagccatc atgttgcgag tccctgctaa tgttattggt gacagtatcc
     8401 gaacagtgaa agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc
     8461 gtgcaccttt agaattgatc caattaggtg aattagaaca attgatgttg aaagaacaag
     8521 ggttgacgat cgaatcagtt ccccaagcca atcaaattgt ttatctaagg gagaactcaa
     8581 atatcagtac aggtggtgat tcgatcgata tgactgatga attttctgaa gcttacaaaa
     8641 aaatcgccgt ttctgcggta gaagcactag gagctaagat tagtggtatc gatttgatta
     8701 taccagataa agaaatcgat cctacaactg ataaaaaagc atatgggatc atcgaagcaa
     8761 atttcaatcc agccatgcat atgcatgttt atccttttgc aggaaaaggc agaagattga
     8821 cgatgaatgt attgaaattg ttatatcctg aagtatttta aaagaaaaag cttttgacag
     8881 attgtcctat ttgatgtcaa tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt
     8941 tttttcgttt taaaataaac gatcccagaa gctttttttc ttttttggtt tttctttttc
     9001 agctggcgaa gagttttctt ctgggtattt tttttcgata tcaatcacag gttcatttac
     9061 tgcttctttt gctgttaaga gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt
     9121 gactacagtg aaacggctat tgtttttcgt tgctagctgg atatattggt tttggacaga
     9181 aaggggtaga gccccattca agagtatgga agcatctgga tgttttttga gttcttttaa
     9241 aaagttttct ttgttttttg gttggatcat ttcagcaaca gtcatactaa gatagcagcg
     9301 ttccctgaat gtccctaaat atttatgctg ttcttcaggg ttcactaaag gtgtaccata
     9361 aagtccctta tccaaatggc tttttacatc atcttgtgcc ataaaaaata ccctccttct
     9421 tctctttgtc tttcttacga ttatagcata ttcccaataa tacctaaaac atatgaaaaa
     9481 gcggttgctt ttgttcaacg attcacttaa aataagaatg aaaatcattc tcaattagaa
     9541 aggtggattg gatgcgctac ggacgactag tagcattatt agttatttta agtagtattt
     9601 cattgttcat cggagtatcg aatgtacaat tgtcagaact gctccagtta gatgggcaaa
     9661 gcttacaaat cttgctagtg actcgtttgc cacgaacgat ttgtttgatc cttgtgggag
     9721 cgaccagtag tatttgtggg ttgatcatgc aacatttgac ccaaaataaa tttgtttcac
     9781 caacgactgc gggcacgatg gatagtgcgc gtttaggtat tttagtggct atgattttcc
     9841 tacctggtgc atccttgatt gtccgatcct tgacagcatt ttgctttgct tttgcaggaa
     9901 cattgctttt tttatccctt acgcgattgt tcctccagaa aaatcaattg atgcttccgt
     9961 tgattggagt catgttaggg aatatcattg gttcagttgc tacttttttt gcttatcaat
    10021 tccagttggt acaaaatatg acttcatggc ttcaagggaa ttttgcgact gtgatacgag
    10081 gaaattatga gttattgtat gctgttgttc cattgttgct cttggtttgg ctatttgctt
    10141 accgatttac gatcattggt ctgggcgaat cgtttgcagt aaatctggga atagattacc
    10201 agaagatgca gtttttaggc attggcttag tatcattggc tagtgcagtc atgttgatca
    10261 tggtaggaag cattcctttt atcggagtga tcattccgaa tcttgtctct agaatatatg
    10321 gagatcaagt gcacaaaaca attggcgtaa ctgctttagc aggtagtttg ttcttagtat
    10381 tttgcgatct tgtcgctcgc gtgttgattt ttccttatga aataccagtc agtgtgatcg
    10441 ttggaatcat cggtggggct atcttccttt atcttctgat aaggggacga cgatatgctt
    10501 aaaaaaaagc catctatttt tattggtatg cttgttcttg ttttatttgt tacaggaatg
    10561 tacttgacga tccatacata tggaaactgg cattttgcat gtgcattgag gagtaagaaa
    10621 atttttgcat ttatccttgt ctcgctagct gtcagcagtg ccacaatcag ctttcagaca
    10681 attacgcata accagttttt gactccttca gtattgggac ttgagtcgtt atatgtcctt
    10741 gtccaaacgg tccttttctt ctttatcgga ggagtgacga tgcttcagca gacaggtaca
    10801 gtttttttct tattgaacgt tgggctgatg gtcgggctga gtctattgtt atttttcttt
    10861 cttttgaaaa aaatgagagc gaatcttttt ttattactga tgtcagggat gattttaggt
    10921 acgctttttg gtagtgtcag taccttttta caagtcatca tggatccgaa tgaatatgat
    10981 ttgcttcaag gcagattatt tgcaagcttt ggcaatatcc agagtcagta tcttttgcct
    11041 gcttctttga tcattatcgg ctgctggtac ctgttatttc gatatcgaca tgtgctagac
    11101 gttttccatc tcggaaatga acaagcagta aacctcggga tagacacaga tcgttttgaa
    11161 tttttgattt tatgttgtgt cagtcttttg acagggacat caacggctct tgtgggtcca
    11221 gttacttttt tagggtttat tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt
    11281 catggtcagc tatttatcgg aagctttctg gtagctatgc tgtttctgac aggaggacag
    11341 ctcgcagtag agcagatttt cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc
    11401 ggaggaattt attttgtcac aaaattgatt cgcgaaagga agagtatcct atgattgatt
    11461 tacaaaaggt caataaactg taccatggaa aatcagtggt taaagatgta cagttaacga
    11521 ttcctgaaaa gaagttgact gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt
    11581 tgtcgatgat cagtcgtttg ataccaaaag atacaggtga aatctattta gaccataacg
    11641 aagtaaaggc ttggaaatcc aatgaattag ccaaaaaatt atcgatccta cgacaatcga
    11701 atgaaataaa tgtccaaatc accgtgaaag aattagtaga atttggacga tttccttata
    11761 cgaaaggaaa attgacgaca aaagacgagg aaatcgttca ggaatcactg gaacacattg
    11821 ggttagaaga aatagcggat caatcgattg acactctttc aggcggtcaa ctccaacgtg
    11881 cgtatattgc aatggtgcta gcgcaagaca cggattatat tttactggat gaacctttga
    11941 acaatctaga tatgaatttt gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag
    12001 aaatcgggaa aacaattgtt attgttcttc atgatatcaa ctttgctgca agttatgcag
    12061 acgaaattgt tgcaatgaaa gatggacagt tatttacgca aggaacgacc caagaaatga
    12121 taaaaaaaga tgtattggat gaattatacg aaatggacat acggatttgt gagattgaag
    12181 gccgcagatt ctgtctttat ttctaaaact aacattagga gagaagagat gaaaaaatta
    12241 gtagtaggta tcttgattct agcatcattt ggtttagcag catgtggaaa tagcaacaac
    12301 accagtcagg cagacacaaa aaaaagcagc acacaaactg aaactacatt gacgataaca
    12361 gatagtaatg gagaccaaat cgaggtgcca aataatccag aaaaagtcgt cgtattcgac
    12421 aatggttctt tagatacgat ggatgctctt ggtgttggag ataaggtagt tggagcagct
    12481 acaagcagcc ttcctgagta tctttcatcc tataaaaaag tagaatcagc aggtggaata
    12541 aaagaaccag acttagaaaa aattaatcag cttcagccag atctaatcat catttctggc
    12601 cgacaaagag attttcaaaa agatctaagt caaattgctc cgaccatttt cctttcttta
    12661 gatgcgaaaa atccatggga atctttccag caaaatgtca ctgctttagg ggagatcttt
    12721 ggtaagcaag aagaagctaa aactcaatta gaggaattat ctagtgcaat cgatcagacg
    12781 aaaaagaaag cagaagctac tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa
    12841 ttatcagcct acggatctgg ttctagattc ggtttcatcc atgatttatt tggctttgaa
    12901 caagcagatg atcaaatcga agcatccact cacggtcaaa gtgtttctta tgaatatgtg
    12961 ctggaaaaaa atccggatat cttatttgta gtagacagaa caaaagctat tggtggagac
    13021 gacagcaaag atgatatttc tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat
    13081 cagcagatca tctcccttga accagatgtc tggtatttaa gcgggggcgg tttagaatca
    13141 atgaaactga tgatcgaaga tgtgaatcaa gcatttaaat agcataaata acagaggatt
    13201 aaactctcat actatgtaac aataatgttt cttacattct taaatagcaa gagcctatcc
    13261 tcaaagaagt tatcaaaaac catccgtaaa atcgtattga tgattttacg gatggttttt
    13321 atttatttga gaaatctttt ttcaacgtga attcattatc tacatcatac gggttgattc
    13381 tagaatgggg agtggcaaaa aatagtgtga tccatttttt cagcatcaag atcgcctcct
    13441 ttgatttgaa tcaaaggtac aaagcgaaac ttagataaaa cttaaattta gatattttta
    13501 ttttgtaccg atctcttctt taaacgatgt tccatctgct gcttctcggc aataagtcca
    13561 ctagactaga aaatatgaga agctattttc tgtccagcgt tcttattact caaaacaaat
    13621 cgcagttgtc acaatctctt ctaaacgatg ttccatctgc tgcttctcgg caataagccc
    13681 actagactag aaaatatgag aagctatttt ctgtccagcg ttcttattac tcaaagcaaa
    13741 tcgcagttgt cacaatctct tctaaacaat gttcaatcag ctgaccttcg gtattagctc
    13801 aaagcactga tttcacaacc tctatttagg catgtgtctc agcaagcggt tgatagttga
    13861 tgatctgcat ttttcgataa aaatcggcgg gaagcttact ccaccgtttt agtaattgat
    13921 agaaataact ctcttctgct ttgattttat actcgcttaa tatcaatcca aaactattcg
    13981 ttttttttga agctaatttt ttataaatat ccaatgcttt ggttttatct tcattgacaa
    14041 agagtgtttt tacttcaata taagaaccct cgatattttg tgtttcttcg ataaaataga
    14101 tgttttttat atattccttc atagtgattc ctccaaagaa gtcttttatt tgataagttt
    14161 actctaccga agagttgtta ataatccgtg aacaaaaagt aattcttttt ccaatagttg
    14221 ttaaaggttt tttatttttt atagtatttt ttgttattta agaaaggaat aaggttaaac
    14281 aaataacagg ttgctttttg ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa
    14341 agttgtctct tttgtgataa gagaaatcat aataaacgaa tgacttactt tccaataaat
    14401 tgagaacagt cagaaccaga caaattagtt gcatctgatg aataaagtga ttattctatg
    14461 acatgaggtg atacagatga aattagaagt gacaaaagaa gcacaagaag tattgaagaa
    14521 taaattagaa gcagacgatc aacttttatt agatatcgaa aacggtgacg gtccatttgc
    14581 agaaagccaa gtcagttgcc aattggatac ggcatttcga ttgatcattg tcaaaaaaga
    14641 tacacaaaca gatctaagtc tttattctgt agtggctgat acaccagttg gaccaatcaa
    14701 aatcaaagac agtgcaatac tttacatgga tgatccatcc acacttgctt tagaaccaac
    14761 ttataatagt ttacaattaa aaggaccaag cggcttgaga aaaggcaact tgcaagttgt
    14821 tcgaaaagac tagagggata aaatgggaaa attggcgatc atcagcgacc tacatgtgga
    14881 catcaataaa ttcggcgaat cagagctgat gcagctttgg caagttttgc aaaagcacaa
    14941 gatcactcgg cttcatctag ccggagatac ggctaataaa gtagaccgtt gcctagctgt
    15001 tgtcgaattt ttcgtcaata aaggattatc tacgacattt catttcggca atcacgagtt
    15061 ggcagatgtg cccaatgaac agatgatgga ccattttcca gacccacatt ttttaaatga
    15121 acggtatatt gctttaaatg agcagaccgt tcttttaggg atgaatggct ggtacgacta
    15181 tcagttttcc gaattgaaag attacgaggc aatcgtacga atgaagcgtt tgtattggta
    15241 tgatcggttc atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc
    15301 tgcaaaaaat ttgctagacc agctggaagc aaaaaaactt gaggtgattt tatccactca
    15361 tttcgttcct aaacgggcat ttatcatgta tcaatctgct ccttatgagc gttggaacaa
    15421 attaaatgca tttttaggat cagaatcatt cggagaatta cttgaccgtt attcaaatat
    15481 caaacaagta gtttttggcc atacgcatcg tcgctttgaa gataaatcca tcggtcacac
    15541 gatttacagc tgcagacctt ttggttatta ttatgaatgg cagctgaccc gagattttgt
    15601 cttgaaagaa cagttgctag acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc
    15661 ccatcaccca gcttttcagg cctatcaaac acaacatttg caagaggaat ttgaacaagc
    15721 gatgacaatc gttccgtatt gagacagcat tagtaatgtg taaaagaaaa taccttgtaa
    15781 aacataagga taagtaagag cagtagatcc atttatcgtg gatatgctgc tttttttgcg
    15841 gaaaataatc agcgaatgtt tatttgatcg gatgatttgt aactcgcaga gtactagatg
    15901 caagcgtgta ttcttttttt gaacgataat cacaaaaaaa aatataatat acatttttta
    15961 tttaattata attaatattg attgaagtct aggcagtcaa gt
//
LOCUS       DAJSVD010000060        15946 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSVD010000060 DAJSVD010000000
VERSION     DAJSVD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.061"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            292..1431
                     /locus_tag="ON498_001748"
     CDS             292..1431
                     /locus_tag="ON498_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR3939997.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="ON498_001749"
     CDS             complement(1498..2073)
                     /locus_tag="ON498_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3939998.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="ON498_001750"
     CDS             complement(2078..3052)
                     /locus_tag="ON498_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR3939999.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="ON498_001751"
     CDS             3335..4303
                     /locus_tag="ON498_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3940000.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="ON498_001752"
     CDS             4416..4694
                     /locus_tag="ON498_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3940001.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="ON498_001753"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="ON498_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3940002.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="ON498_001754"
     CDS             5151..5906
                     /locus_tag="ON498_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3940003.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="ON498_001755"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="ON498_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR3940004.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="ON498_001756"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="ON498_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR3940005.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="ON498_001757"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="ON498_001757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3940006.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="ON498_001758"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="ON498_001758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR3940007.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="ON498_001759"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="ON498_001759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3940008.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="ON498_001760"
     CDS             10643..11185
                     /locus_tag="ON498_001760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3940009.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="ON498_001761"
     CDS             11208..12764
                     /locus_tag="ON498_001761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3940010.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="ON498_001762"
     CDS             12778..13680
                     /locus_tag="ON498_001762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3940011.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="ON498_001763"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="ON498_001763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3940012.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="ON498_001764"
     CDS             15123..15545
                     /locus_tag="ON498_001764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3940013.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5265 a         2877 c         3444 g         4360 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcct
//
LOCUS       DAJSVD010000061        15606 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSVD010000061 DAJSVD010000000
VERSION     DAJSVD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.062"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(58..285)
                     /locus_tag="ON498_001765"
     CDS             complement(58..285)
                     /locus_tag="ON498_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3940014.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(388..2976)
                     /locus_tag="ON498_001766"
     CDS             complement(388..2976)
                     /locus_tag="ON498_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR3940015.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2996..3655)
                     /locus_tag="ON498_001767"
     CDS             complement(2996..3655)
                     /locus_tag="ON498_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3940016.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="ON498_001768"
     CDS             complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="ON498_001768"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3940017.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4428..5294)
                     /locus_tag="ON498_001769"
     CDS             complement(4428..5294)
                     /locus_tag="ON498_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3940018.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5403..6044
                     /locus_tag="ON498_001770"
     CDS             5403..6044
                     /locus_tag="ON498_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3940019.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="ON498_001771"
     CDS             complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="ON498_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR3940020.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7485..8384)
                     /locus_tag="ON498_001772"
     CDS             complement(7485..8384)
                     /locus_tag="ON498_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3940021.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8386..9183)
                     /locus_tag="ON498_001773"
     CDS             complement(8386..9183)
                     /locus_tag="ON498_001773"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR3940022.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9161..9865)
                     /locus_tag="ON498_001774"
     CDS             complement(9161..9865)
                     /locus_tag="ON498_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR3940023.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            9996..10571
                     /locus_tag="ON498_001775"
     CDS             9996..10571
                     /locus_tag="ON498_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR3940024.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10689..11366
                     /locus_tag="ON498_001776"
     CDS             10689..11366
                     /locus_tag="ON498_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3940025.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(11445..13253)
                     /gene="pepF"
                     /locus_tag="ON498_001777"
     CDS             complement(11445..13253)
                     /gene="pepF"
                     /locus_tag="ON498_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR3940026.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(13429..14427)
                     /locus_tag="ON498_001778"
     CDS             complement(13429..14427)
                     /locus_tag="ON498_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR3940027.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(14653..15306)
                     /locus_tag="ON498_001779"
     CDS             complement(14653..15306)
                     /locus_tag="ON498_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR3940028.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         4476 a         3365 c         2697 g         5068 t
ORIGIN      
        1 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
       61 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
      121 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
      181 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
      241 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
      301 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
      361 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
      421 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
      481 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
      541 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
      601 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
      661 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
      721 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
      781 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
      841 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
      901 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
      961 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     1021 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     1081 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     1141 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     1201 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     1261 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     1321 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     1381 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     1441 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     1501 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     1561 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     1621 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     1681 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     1741 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     1801 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     1861 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     1921 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     1981 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     2041 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     2101 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     2161 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     2221 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     2281 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     2341 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     2401 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     2461 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     2521 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     2581 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     2641 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     2701 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     2761 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     2821 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     2881 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     2941 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     3001 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     3061 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     3121 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     3181 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgttgg gcaaccgcat
     3241 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     3301 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     3361 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     3421 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     3481 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     3541 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     3601 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     3661 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     3721 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     3781 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     3841 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     3901 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     3961 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     4021 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     4081 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     4141 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     4201 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     4261 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     4321 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     4381 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     4441 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     4501 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     4561 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     4621 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     4681 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     4741 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     4801 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     4861 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     4921 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     4981 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     5041 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     5101 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     5161 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
     5221 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
     5281 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
     5341 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
     5401 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
     5461 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
     5521 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
     5581 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
     5641 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
     5701 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
     5761 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
     5821 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
     5881 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
     5941 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
     6001 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
     6061 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
     6121 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
     6181 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
     6241 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
     6301 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
     6361 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
     6421 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
     6481 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
     6541 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
     6601 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
     6661 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
     6721 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
     6781 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
     6841 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
     6901 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
     6961 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
     7021 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
     7081 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
     7141 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
     7201 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
     7261 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
     7321 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
     7381 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
     7441 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
     7501 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
     7561 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
     7621 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
     7681 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
     7741 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
     7801 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
     7861 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
     7921 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc agatgtatcc
     7981 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
     8041 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
     8101 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
     8161 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
     8221 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
     8281 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
     8341 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
     8401 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
     8461 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
     8521 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
     8581 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
     8641 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
     8701 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
     8761 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
     8821 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
     8881 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
     8941 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
     9001 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
     9061 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
     9121 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
     9181 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
     9241 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
     9301 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
     9361 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
     9421 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
     9481 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
     9541 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
     9601 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
     9661 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
     9721 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
     9781 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
     9841 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
     9901 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
     9961 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    10021 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    10081 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    10141 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    10201 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    10261 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    10321 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgggctgaat
    10381 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    10441 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    10501 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    10561 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    10621 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    10681 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    10741 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    10801 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    10861 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    10921 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    10981 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    11041 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    11101 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    11161 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    11221 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    11281 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    11341 gccatttaca cttgctttct aattaaaaaa atacgtcgac tcccttacaa gagagccgac
    11401 gtattttttt cgtttctatt cgagaaaatc aaaaatagaa agccttataa acgatcgatc
    11461 aattcttcta attcatttag acgttgttca aacatcgaca ttgcatcttc aatatatgca
    11521 gcctgtgtca tatcaactcc tgcttttttc attacttcca caggataatc gctgttacct
    11581 gcttttaggt aagccaagta gttctctagt gcctctggtt cctggtttag tattttcttc
    11641 gccagtgctg aagcagcaga aaagccagtc gagtattgga aaacatagta attataataa
    11701 aaatgcggta tccgtgacca ttcgaattta atttctgggt cttcttcgac tgctggacca
    11761 taatattttg cattcaattt accataacta tcacttaggt attcactagt caatggcaca
    11821 cctttttcat cttccgtatg catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa
    11881 actgttcctt taaacccatc caagtaatgg ttgagtacat aagcccgtac acgagggtct
    11941 ttttctgttt ccaataaata ctccgttagg atattttcat tcgttgtcga agcaatttct
    12001 gctaaaaaga tggagtagtc gccgtacaca taaggctggt tcgaacgagt gaaataacta
    12061 tgaacactat gtcccatttc gtggacaagc gtaaatagct gatccagcgt atcatgccaa
    12121 ttcaataaaa tatatggatt tgtgtcatag cttcccgaag aataagcatc gcttcgtttc
    12181 cctttatttt cgacaacatc gatccaacgt tcagagaatg ctttttctac gatggccatg
    12241 tattcttcac ccattggttt cagtgcttct aaagcttttt cttttgcttc ttcgtacgta
    12301 aaggtaattg gagcttcccc taagaccggt gtataaagat catacatgtg cagtttttcc
    12361 acttctaata aacgtttccg taattccatg tatcgatgta acaaaggcaa atgcttgttt
    12421 accacgtcta ccaaagtatc gtatacactt tcaggaatat gattattgct caaagacgct
    12481 tctctggcag agctgtaatt acggactttc gctttaaaat tatgtccttt tatatgtgtg
    12541 cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa tcccttaaat
    12601 gctgcttcgc gcacccttcg atccgtgctt tctagcaact gaccatacac gccatgagat
    12661 aattggacta tttcaccatt ttctccttca atcgttggaa aaactagatc tgcattattc
    12721 aaaacagcaa atgtatcact tgaagcatca aagatttcac ctgctccagc aagaagagat
    12781 tcctgctcag cagataagac atgggctcga ttatctacta tttgctggat ataatggcga
    12841 tagacttcca attttggttc ttctttgaaa tattgccaaa tctggtcatc tgacaattgc
    12901 aatatttctg gttcaaacca ggaaacggct tcactaactt tcgaaaacag actgcttgct
    12961 cttgcataaa gcgcttggta atctgtattt ccagtgtctt gatcgttttt aaggtgcgca
    13021 tatacataaa tgacttcagt ttggcgataa accctcaata cgaattcaat cgcatttaaa
    13081 aattgagaag cgccttgatc aagtgttcct ttgtgttttt cagattgttt taactcttct
    13141 gataattcca aatatttctc atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc
    13201 caagttaaat tttcaggcaa ttcttctcgt tttggcaact gctttacttc catgaaatcc
    13261 actccttttc ttcccttagc tctatctatc aaaatagatc atagtctaac atccacatgt
    13321 cacagcatct aacttttcta ctcattcatg tgtctgcttc catcaatttt agcataacta
    13381 aatcttgttt tacatagaaa accgattcct agcctatttt tatacttact attttttcaa
    13441 agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag accatctctt catcaatcat
    13501 aggaaaaagc cattcccttt tatgttcttt tatttggcaa agccatgagt tgaaatcagg
    13561 aactttctgg ttttccaagg cttcttcgta caacattcga taaagaaaaa cctgttcttg
    13621 taaataaaag aaaaaccgac ttttttgata gatccacgat gatagatgca aaagatgttt
    13681 atgacgcaag tagcattgtt cttgtatgga tactgtattc ttttgcttgt gaataagttg
    13741 gcgtgtcagc catttcttcc catcttcact cactttttct atttttttca tttggttcat
    13801 tttaatttcg ttactagtga atatttcttt aaggtcccta ctagaaaaag tccagcagct
    13861 actgcaaaag gaaatatgcc ctgatacagt ctctgtaata tgatgataca aataaagctt
    13921 ttgtttcttg agatcagctt tccagcaatg cactcctctt tttctattat agtagcagca
    13981 acttttttct atcaatgaga attgcgattg tccaagaaaa gaattaccaa gtatccacca
    14041 tacttggtaa ctgttattca aataattttg ggttctttca ataaatctct ggtgagacag
    14101 tctagaacat tggatttcta ttgctagatt cgccaacaaa atatccggcc tttgccgcaa
    14161 cctcggcaaa taggcttcca aaaatactgg ttctactgac tgtcccagcc aatccatgaa
    14221 gcattccttt aactgtaagt gctcttttga ttctctttca ctaaagaccg aacagttatc
    14281 tgatttttga tgggcaaaat gagcacattt tttctttccc tttttcaaaa tcactctttt
    14341 tcgacagcca cgacaataat atttctttcc tttttctgca tgtttagcaa aacagcactt
    14401 attatattca ttcattgcaa gcagcatttt tctccctctg aacttattta cgtctaagaa
    14461 tttatttaca cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta ataagaacag
    14521 ctaaacaaaa atagttccca atatttttcg tttggttggg cttgttaccg cagatgcaag
    14581 cttttgaaca catttatacg aaaaaaagac tgcgacacaa tttttgtcac agtctcttac
    14641 tataatgctg atttatacaa aataaaattt cgttcgctcg atagcattac gcgccatcag
    14701 acattgtcca tattcattca atgtttctgg tgtacgatcg ctcttttcag caaactccaa
    14761 aattcgagca atctgattct ctacatccgc ttggttgaga ttttccatcc agaagtgaac
    14821 gactaggtaa tagcgctctt cgaataaata caaatcagtc caagcagact ctagttcgac
    14881 atctgttgca agttggatca cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc
    14941 ctgttcttct agggcgcttc cagataattc ttcctccgga tcgctgccaa taatctgctg
    15001 acgaagaaag tcactcacat cttctgaatc aacagtagaa tcttctgatt gagaaatttc
    15061 ttcatccgga agatttttac tgataaacag ttctaatccg tctccttttg gcaaaacttg
    15121 gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata tcaacttctt ccaaaatact
    15181 ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg
    15241 ttcctctaaa tcttcatgac caattagtac acggatcgta ttttcattaa tatgttccat
    15301 ttccattttg ctacacctca ctttgccaca atgtttaata tagattcatt gtagcgaaat
    15361 cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa accagcggat
    15421 ttttcaacct ttcaagctat cttatttaac tataacattt ttggcagacg aatactatat
    15481 agccgacttt tcagctaatt ttccctctct atttttcgtg caatcatcat cttctactac
    15541 ctttttatac atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat
    15601 ctttgt
//
LOCUS       DAJSVD010000062        15393 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSVD010000062 DAJSVD010000000
VERSION     DAJSVD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.063"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(292..364)
                     /locus_tag="ON498_001780"
     tRNA            complement(292..364)
                     /locus_tag="ON498_001780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(329..331),aa:Lys,seq:ctt)
     gene            745..1314
                     /locus_tag="ON498_001781"
     CDS             745..1314
                     /locus_tag="ON498_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR3940029.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1301..2161
                     /locus_tag="ON498_001782"
     CDS             1301..2161
                     /locus_tag="ON498_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3940030.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2351..3055
                     /locus_tag="ON498_001783"
     CDS             2351..3055
                     /locus_tag="ON498_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3940031.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3060..4895
                     /gene="walK"
                     /locus_tag="ON498_001784"
     CDS             3060..4895
                     /gene="walK"
                     /locus_tag="ON498_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR3940032.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4892..6208
                     /locus_tag="ON498_001785"
     CDS             4892..6208
                     /locus_tag="ON498_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940033.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6209..7078
                     /locus_tag="ON498_001786"
     CDS             6209..7078
                     /locus_tag="ON498_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR3940034.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7133..7942
                     /locus_tag="ON498_001787"
     CDS             7133..7942
                     /locus_tag="ON498_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3940035.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8045..8674)
                     /locus_tag="ON498_001788"
     CDS             complement(8045..8674)
                     /locus_tag="ON498_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3940036.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8844..10136
                     /locus_tag="ON498_001789"
     CDS             8844..10136
                     /locus_tag="ON498_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940037.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10212..10874)
                     /locus_tag="ON498_001790"
     CDS             complement(10212..10874)
                     /locus_tag="ON498_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3940038.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11099..11383
                     /gene="groES"
                     /locus_tag="ON498_001791"
     CDS             11099..11383
                     /gene="groES"
                     /locus_tag="ON498_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3940039.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11434..13059
                     /gene="groL"
                     /locus_tag="ON498_001792"
     CDS             11434..13059
                     /gene="groL"
                     /locus_tag="ON498_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3940040.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13148..14296)
                     /locus_tag="ON498_001793"
     CDS             complement(13148..14296)
                     /locus_tag="ON498_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3940041.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLTLHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14398..14982)
                     /locus_tag="ON498_001794"
     CDS             complement(14398..14982)
                     /locus_tag="ON498_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR3940042.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15109..>15393)
                     /locus_tag="ON498_001795"
     CDS             complement(15109..>15393)
                     /locus_tag="ON498_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR3940043.1"
                     /translation="LSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNI
                     YIENELTDESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         4995 a         2643 c         3190 g         4565 t
ORIGIN      
        1 attaaatatt cttcactttt taacctttta tagtacttag atttaatcct atatgtttag
       61 agagttcatt ggcagcatga ttttgcaaag cttctcgaat tattgttata ttagaaactt
      121 tactgttcat aattatgctt cttttttaac tcaaagtcca aagggctaca gtggactttg
      181 agttaaaaat aaaaagccct agttattttg aaaaataaaa aaagaacagc gtaaatgttt
      241 tcgaaatgct ttcgcaccaa catttacctg ttccgtgggt ttggcaccca atgggccgtg
      301 aggggctcga acccgcgacc cgctgattaa gagtcagctg ctctaccaac tgagctaacg
      361 gcccgtacaa aaagtactta ataatagtag cactaagaat tttaaaatgc aagactacat
      421 ttaaagaaaa tcagaaaaat ctttacttcc ttataggaaa tcattcatag attattcaca
      481 tttgctcgct attatataag cataccaaca aacaaagttc ttgcaagaga acaaacccta
      541 acactttttt cattttttac tcctttcccg ccgtctcccc ggcgggtctc ctgttcttaa
      601 cgataaaaga caagaaggct tagccttctt gttttttgtt ttcatggaat cccgctaatc
      661 aaatcaccta gttaaagcaa ctagattgaa tgtttatcat aagtctgata tcatgtttat
      721 gaaatgaaaa aaggagcggt tatgatggaa aaaatgcgtc agcaattaat ggagtggggg
      781 aaggaactag aaacattccg tctgcctcac tgggaagaac tccctgactt agaactttat
      841 atggaccaag tcagaacgtt agttgatcgt tatctttcac ctgtgataca aggagaaaag
      901 catccattgc ttacctcttc tatggtaaac aattacgtta aactaggttt gataccagca
      961 ccagttaaaa aaagatataa caaagaacac gtagcatttt tacttgcaat cactacgttg
     1021 aaacaagtat tgactattcc tgaaatcaaa gaagggatat tgttccaagg aaaaacagta
     1081 ggaattcgtg aagcctataa tctattttgc gatgaacaag aagcagctgt gtggatggtc
     1141 agccaattag cacaaggtaa atctcatccg caaaaattcg accaggcaac ccctgtagaa
     1201 tatattgctg taagagcggc aactttatcg tttgcaatga aacttcttgc ggaaaaaacg
     1261 attgttctag aaaccgaata tttaaaagag gagaaaacaa atgaaaaaca ataaaatcgc
     1321 aattttagta gattcaggta cggatgttcc agaagaattg ataaaaaaat acgagatgta
     1381 tatgatccca ttgaaaatta tttataaaga tcaggtatat acagataaaa ttgacattac
     1441 ccctgaagag atctatcaac gtttgccaga ggaaatccct ggtacatctt tgccggatgg
     1501 tgagtcgatc acagaaatct ttgagcaaat caaaaaagac ggttatgaaa aggtacttgc
     1561 ggtaacaatt tccagtggat tgagcgggac atataatgtg gttcgtttaa ttgcacaaca
     1621 gcaagaagaa ttggaagcat atgtactaga tacgaaaaat attggtattg gtgcaggttt
     1681 ttctgcgatc caagcagcaa aatggctgga agaaggagta gaatgggatc aactgatcag
     1741 taatctgaat gagttagtcg aaagaacaaa agttttcttt aatgttgcga cattggaata
     1801 tcttcaaaaa ggtggacgga tcggtttagt tgcttctatt gtaggaaccg ctttaaagct
     1861 taacccaatc atctcatgta atgaagaagg gatttattac acagtaggaa aagctagggg
     1921 acgtaaaaaa agtttggata aaacggttgc ttacgtcaaa gaaaaaatcg gtaatgcaaa
     1981 ggtcttcaac ttagctgttg cccatgggga tgcgaaagaa gaagcaattg aaatggaagc
     2041 gagacttaaa agagagttcc cacaagcaaa ccaaatctac tttggccaga tttctccggc
     2101 attagtagtc cacactggtc cgggattatt gggtgtcggt gtacaagttt tagaaggata
     2161 aatcaaaaaa aggaaacact gaggtgaaaa tcagtgtttt ccttttttat tgaagttaga
     2221 acaaaaaata ataaagtatt ttgcactttc attttaattt tctcttttgt tttataggca
     2281 aagatgaaac aaaagcagaa atacggtata atgagaaggt atgacaataa tatataagag
     2341 gtggctcttt atgaaaaaag ttttagttgt cgatgatgag aagccgattt cggatatcgt
     2401 caaattcaat ttagccaaag aaggctatga cgtttatact gcatatgatg gggaagaagc
     2461 cttggaaaaa gttgctgagg tagaacctga tttgatttta cttgatttga tgctacctaa
     2521 aatggacggc ttagaagttg ctcgtgaggt acgcaaaact tatgatatgc cgatcattat
     2581 ggtaacagcc aaagattctg aaattgataa agtcctaggc ttagagttag gcgccgatga
     2641 ttacgtgacc aagccatttt ccaaccgaga attagttgca cgtgtcaaag caaatcttcg
     2701 tcgcggtgcc acggctgcga aggaaccaga agaggcagca cctgcggaat tgacgattgg
     2761 tgatttgacg atccatccag aagcttacat ggtgacgaaa cgaggagaaa caatcgagtt
     2821 aacacatcgt gaattcgaac tactctttta cttggcgaag caccttggac aagtgatgac
     2881 aagagaacat ttattacaaa cggtttgggg ttatgattat tttggagatg tacgaacagt
     2941 ggatgtaact gtacgtcgtt tgcgtgaaaa gatcgaagat aatccaagcc atccaaatta
     3001 tttagtgaca cgtcgtggtg tcggttacta tttgcgtaac ccagaacagg agtaattgga
     3061 tgaaaagaaa agtccgcttt tttcgatcag tcaattttaa gatcgctatt acctttattc
     3121 tgattttatt gatctcgatc gaaattattg gtgcctactt tatacgtggt ttagaacgtt
     3181 ccacgatcaa tacatttatt aaagatatga atcaaacggt ggaatcatta gcaacaacaa
     3241 tcagcccgga attgaatcga aaagataatg cagatgatga agaggtaaat gcgaatatca
     3301 aaagatttat tgaaaacagt gcgactagcg atatcatcga aatacgggtc gttgacgaaa
     3361 aagggatcat ccgaggaaca accgatgtca atgaacaaag cgctgtggga aagaaaaatg
     3421 attatgttga tatcaatgat ttcacgacta aacgttatgt tgctttagat aacgacgata
     3481 aacgggtgaa tatcaatgtt caaccgatcc tgtccccgac cggcgatgcc gttatcgggg
     3541 ctctttacgt caaaagtaat atcgaacaaa aatattcgga aatcaataac acagcagtca
     3601 ttttctttac tgcctcacta attgcggcat ttatttcaat gatcgtatct gttttagttg
     3661 ctcgttcgat tacacagccg atcggtgaaa tgagagaaca agccattcga atcgctagag
     3721 gagactacag tcggaaagtc aaagtgcatg gacaagatga acttggacag ctggctgata
     3781 ccttcaatca gttagctgaa cgtattgaag aagctcaaga tacaatggaa gctgaacgca
     3841 atcgtttgga tagcgtgcta tcccatatga cagacggcgt cattgcgaca gatcgcaggg
     3901 gtaaagtcat tacgatcaat gatatggctg tttccttgct agatatcaaa aacgaggaag
     3961 caatcggaca atcgattttg acacttttag atatcgaaga agaatatacg ttgcggaaat
     4021 tattagaaga tccaaacgaa atggtacttg accgttcaac cagtacattg gaaagtgatc
     4081 agatgatctt gcgagtagac ttttctatga tccgtcgcga atcaggtttt atcagcggat
     4141 tagtagctgt cttgcatgat gtgacagaac aagaaaaaac agaacgggaa cgtcgtgaat
     4201 tcgtctctaa tgtttcccat gaacttcgta caccgttgac aagtatgcgt agttatatcg
     4261 aagcattgag cgaaggtgcg tggaaagacg aggaagtagc accaaatttt ttgaaggtaa
     4321 cgctagatga aacagaccga atgattcgaa tgatcaatga cctcttaaat ctatctcgca
     4381 tggatacagg taatacccag cttcaattag aatatgtcaa tttcaacgag ttagtgaatt
     4441 ttgttcttga ccggtttgat atgatggtgg gaaatcagga aaaaaattat aagatccgtc
     4501 gtgaatttac gcaaagagat ctttgggtcg aagtagatac agataaaatc atccaagtag
     4561 tcgataatat catgaataac gcgatcaagt attcaccaga tgggggaacg attacttgtc
     4621 gcttattaga aacgcataat aacgtgattt taagtatcac cgatcaagga ttggggattc
     4681 ctaaaaaaga tctgaaccga gtgttcgagc ggttctatcg agtagataaa gcacgtgcaa
     4741 gagcacaagg tgggacagga cttggattag ctatttctag agaagtgatt aaggcgcatc
     4801 gaggcgctat ttgggcagag agcaaggaag gaaaagggtc gactttttat atctcgctgc
     4861 catatgaacc ttatgaggag gaatggtggg aatgagaatc tctgaaaaag tcgttcgtat
     4921 aggactcatt ttgatggttg cgttaagcat atacttctct tatgcaatct ggctgagccc
     4981 agcaggaaaa agttcaatca acgtagatga gacaaacact caaatgatcg agtctcaaag
     5041 caatcgaaaa acttccgaag tctttcttcc gcttcatgtt acatggcacc attcgggaat
     5101 gatccaagaa accaacagtg aaaatctcgt gtaccgattg caattgatca tcgataaagc
     5161 aagatttgga aagctttctg aagtagtttc aggagacaaa tcacagtttg aacaggtgaa
     5221 aaatctgaac tctggttttg agttggccta taatgcgcct tttttgctaa gagaatacaa
     5281 ggaagcattt gatttagatt tagatgtgtc gaacttggca aaagaaagcg aaaatatgta
     5341 ttttactaga atacaattcg atcaaggaaa taaaaaaatc cgatttttga attattcgaa
     5401 ccatctcgta tatgaagcaa atatttcgtt gaattggaca gatatcaata aagaattaac
     5461 agcaaaagac acaaaatgga cagaaatgac aacagaacca tcgacgatcg acacgcaata
     5521 tgctacgaaa cattcgatca aactgaagaa gtatagttat atcctgtcac agcaacccta
     5581 tacacttttt agaaatgctt ttttccagaa accagatagc gtgaaaaata atgatgaaag
     5641 caatgaattg attttttacg gtgggaatga aacaatgtct atccattcgg aaacgcaagt
     5701 agttgatttt cgtggagagc tgccagagaa aaaagaaaat gctagtgtat tttctgaaag
     5761 tttcccctat attagcaaat taggcagttc tttagggaac ctcagatatt ttgaccgcag
     5821 tgacaatacg atcgattatc ggatattcgt ggaaggcttc ccggtttttg ggacagacag
     5881 cagaggacag attggtgtgg aagttggcgg tgaaacgaat ggttctgtgc aagtgaatat
     5941 ccaaaccagt ttgaatacga ttcaagtgcc gataccttca gacgaagaag tagaacttgc
     6001 aagtacgaat gaaatcatcc agcagttaaa agctaaggga gcagatagca acaagattgg
     6061 ttcagtgatc gttggctata catggcaaaa tgtcaaagaa acgaatcgtg tcgttgatct
     6121 cttacctgaa tggtatgtga aatataaaga taactggtat tcagctaacg accttctgga
     6181 aaaattacct ggattggagg cgaattaatt ggatttcaaa aaaatcgaat ggatattttt
     6241 cgtcgctttt ttagggttga atgtgttttt attcagcagt tatcatgacg cggtatacca
     6301 agaaaacaat gtcattcgct ctaatcagac tgaatcaatc caacaacgtc tggctagcga
     6361 cgatatcact tacacgggga agatttcgtc tgagaacaag caaggctatt atcttagcgc
     6421 ggagcagacc aatcttagca cttcattagc gaattatcgc aaggctggta aaagcggctt
     6481 gctggcaagt ggtgtgtttt tagacggtga tgttttgacg aaatctttat ccagttcaga
     6541 tgctgaaaag aacgtgatcg atccagataa aattagtagt tccttggaag attttttatc
     6601 taacaaagac aatattttat tcggtaatga ttatgtgtac acgaaaaact tttctcattt
     6661 agaagaagaa ccactagaaa tccaagcaag ccaagagtat gaaggcatcc cattcaatga
     6721 tgatacagca aaaatcgttc tttctttaga aaaaaacgat aatgattata gcatcacgaa
     6781 atacatgcag acgcatttat ctgatatcga gcagctgcgc gaaaagacgg aactgtatac
     6841 ggaagaggaa gcaatcaata cattgtacat caataacaaa ataccaagag gatcaaaaat
     6901 tttatggcgt cagctagctt attcctgtat tctgaaagtc cgggaaaaaa atgtgtatgt
     6961 tcctgtttgg catgtagcga tcgaaacaag cgataaagtg gtccaaattg agagcgtcaa
     7021 cgctttcagc aatacgatcg tgacaaacaa tactgtacca aaagtggaag atcaataaaa
     7081 aagcgagtat aatgaaaaaa gtttacaaag taagaaggga agtaggagtc aaatggctga
     7141 agaaaaacca gcttttaaaa tcagtatcct agcaagtgga agttcgggta attcacttta
     7201 tatcgaaagt gaaaaaaaac ggcttttggt cgatgcagga cttagcggta aaaaaatcac
     7261 ttccttgatg gctcaaattg atcggacacc ggaccaatta gatggaatac ttgtgacgca
     7321 tgaacatcga gatcatatac atggcgtggg tgtcttggct cgtaagtatc atttggatgt
     7381 ttttgccaat cagcagactt gggaagcgat ggaaccactg attggaaatg taccagtgga
     7441 acaaaaacat ctatttgaga tggggaaagt cagaacattc ggcgatatcg atattgagag
     7501 tttcggcgtc tcccatgacg cagcggctcc gcaattttat cgtttccata aagaaaatcg
     7561 gtcattcgtc atgttgacgg acaccggata ttgcagtgat tatatgcgag gtgtcattga
     7621 aaatgccgat ggttatttga tggaaagtaa tcatgatctt gagatgttac gcatgggacc
     7681 ttatccgtgg aatttaaaac agcgtatttt aggagataga ggacatcttt ccaacgaaga
     7741 cggtgcactt gtcatgactg atgtgatcgg taatcggacc aaacgaatct atcttggcca
     7801 tcttagcaag gaaaacaata tgaaagaact tgctcattta acaatggaaa atgttttgaa
     7861 agaaaaagat tttggcgttg gacatgattt tgaaatctat gatacagatc cagatgaagc
     7921 aactgagcta ttttcgattt aaacagttca atcaataaaa atataaaaag cagaagataa
     7981 aatcaatcag ggttggtttt atcttctatt tttatttttt tagcatatta aaaaaataac
     8041 cgtattatcg actgactaca ctgatgtgct gcttttcatg tttctcatcc agtatctcat
     8101 ctactaatgc aatcgcgtaa tctgccatgc tgatcgtact gtcgctagca gcgtttcttt
     8161 gtaaataatc atcacttaac aaataagtac ctgtacgttt gccatgtgga tcaaataaag
     8221 aagctggact gatataagtc caatgtagat ttttagattg tttcaatttt aacaatcctt
     8281 cgtacatatt gaaagctgtt ggatagaagg gtgcattttt aggtacttgg tcaatcatac
     8341 gagtcgtctt ttctgtatct aagaataacg aagaggcacc cccaacgaca attaagcgtg
     8401 tatcggttcc ttcaagcatc tcaatcaaat gggacaaaga agtttgatgc atttcttctt
     8461 ttcctttggg agcgttgaaa gcatcaacga caacatcaaa agatgcaaga tcagtagttg
     8521 tcaactcaaa taaatccttt tgtataaacg gaacatctga tggcaatttt tctggatgac
     8581 gcacgattgc cgtcacgtct aaatctctgg acaatgcttc gtctaaaata aatgaacctg
     8641 catggccagt agctccaatg agtgcaattt tcataaaaaa cctccttttt tattcctgtc
     8701 cattgtaagc agttgctgaa tattcgtcaa agaatccgat ttttgaaaag ctcagtgcgc
     8761 attcctttca tggttcgtga tcattgggta tagttaaatt aagaaaactg attgataaaa
     8821 atagaagttg aaggagataa gaaatgaaag agtggttaga tcgtgccttt ttaccaatga
     8881 taacatctgt tgtgaatctt agacccttaa ctgctttgcg taatggaatg acttacacga
     8941 tcccaataat tattgttggc tcgctgtttt tcttatttgc tcattttcct gtggattaca
     9001 tcgctcagtg gatagacaaa acaggatttt ctatgtattt tacacaagta taccaggcta
     9061 catttgatgt gatagccttt tggatcgttt ttgccattgc atacgcttat gcaaaagaag
     9121 aagacatcga tcgcttacca gtcggcataa cagccttgtc tgcttatcta ctgcttattc
     9181 agccactaaa tactcagata gatactgtct ggacaggtgc tgaagggata ctaggagcag
     9241 ttacaactgg gctcatcatc ggatggggct atgcacgttt agtaagactt gcgaaagggt
     9301 ggaagcaaag agagaagata cctgaaaatg tttggcagtc ttttacttca ttgattccaa
     9361 tcattgttat agtcactttt acgcttatcg gttcagcggt ttttcaacat ttgttcagaa
     9421 tgactgttgt tcaaggagta tggacatatg tccagcttcc cattcaatcg ttcatagaca
     9481 cgttgtttgg tgtgctttta ttaggttttt ttgttccgtt tttatggtta tttggtcttc
     9541 atggatctgc gatggtcaat gggttggtta gtcccgtctt gcaagcgaat tcattagcaa
     9601 atgcagaaat cttagcctca ggacaagagt tgacggtggc aaatggcgga catatcgtta
     9661 ctcagcaatt tctcgatcag ttcatgacgg ttacgggtgc tggattgacg ttaggggctg
     9721 tattttttat gacggttttt gcgaaatcga agaaatacag agaattagga aatcttgctc
     9781 ttttaccagc ctttttcaac atcaatgaat caattatttt ttccacaccg atcgtgatga
     9841 acccgatgat ggctgttcct tttattttcg caccggtcgt ttcaggattg attacctatt
     9901 ccgctttata ttttggattc gttcctttat ttacagcagt ccaagtccct tggacaacac
     9961 cgccgatcct ctcgggtttc ttagtaggcg gagtgcctgc agcgatttta caaggtatcg
    10021 tgttgtcaat tagttttttc atttatttcc catttttgaa aaaaatagat tctgcaaact
    10081 gcaaaaagga aagacagaca aaaaatgatg gagcagcaga aaagatgaac gactaaaaaa
    10141 caaaaatact cctgtcgctt tagattggga aaatcaccaa tctaggatga caggagtatt
    10201 tatagtcatg tttatactag ccctttttgg acagctaatt gaacaagaac aactaatgtg
    10261 ttcattccta cgtgtgtgat catcgatgtc cagatccgac cagttgcttt gtactctagg
    10321 ctgaagaaga aacctaggaa aaagtagatt agcagatgac catcattatg agcaaacgca
    10381 aacagcaaag agctgatcac tgctccgacc caaaaatttg aatatcgact gatgatcccc
    10441 agtatactgc ggcggaatac gaactcttcc atgatgggtc cagcaacagt tgtcgccaaa
    10501 ataaaagcgg gtgcttccat gatcatctgg atgatatttt gtgtattctc ggaaggggtt
    10561 gctttgccaa acaatacaga ttctaacatg acagcgatgc tttgcagaat caatgcgaca
    10621 aagattccaa ccaatccata aataacaatc tttggagcag aagctttttt taccgctgat
    10681 tcgatcgcta agggttcacg ttgcttaaaa taaaaaatag ctaggacaac ggcgcctagt
    10741 atatacatga ctgcagttgc tgtgatcaat tcagacgtag attttacaag tcctatcgaa
    10801 gctaggatca agggtgagat aaaaattaga ccatagcaaa aaatgctgag gaagctatat
    10861 ttttttaaag acatgtaatt cctcctttta agggataaca agcatgcaag gcaagcttat
    10921 atcgattgat ttcaattata tcataatttc aataagcagc tgtcagaata aaatttcttt
    10981 tctttgattt aaaagcttgc aaaaaagagg gagaatgagt attattaaaa atgtaggtta
    11041 gcactcgaag gttaagagtg ctaacagatt aaaaaaagag ttatcggagg gatttatcgt
    11101 gttaaaacca ttaggtgatc gcgtcattat cgaagtcgca aaagaagaag aaaaaactgt
    11161 cggaggaatc gttttagctt ctgctgctaa agagaaaccc caaactggga cagttgttgc
    11221 agtcggtgaa ggccgtttgt tagaaaacgg tgaaaaagtt cctgccgctg taaaagcagg
    11281 cgaccaagtt atgttcgaaa aatacgccgg tacagaagta aaatacgaag gaaaagaata
    11341 tctgatcgtc gcaggaaaag atatcatggc gattgttgag taaatttgga agataaaaaa
    11401 ataatcaaaa ataatcaatg aggtgaatca atcatggcaa aagaattgaa atttgcagaa
    11461 gacgcacgcg cagctatgtt acgcggggta gataaattag cagatacagt gaaagtaaca
    11521 ttaggaccta aaggccgaaa tgtcgtacta gaaaaatctt acggctctcc attgatcaca
    11581 aatgatggtg tgacgatcgc aaaagaaatc gaattagaag atcattttga aaacatgggt
    11641 gcaaaacttg tatcagaagt agcatcaaaa acaaatgata ttgcaggaga cggtacaacg
    11701 acagcaactg ttctgacaca agcaatcgtt cgcgaaggtt taaaaaacgt aactgcaggt
    11761 gctaatcctc taggcattcg tcgcgggatc gaattggcaa caaaagcagc agtcgaagaa
    11821 ttgcataata tctcaactgt tgttgattca aaagaagcca ttgcacaagt agcagctgtt
    11881 tcttcgggtt ctgataaagt tggacatttg attgctgatg caatggaaaa agttggaaac
    11941 gacggtgtca tcacaatcga agaatcaaaa gggatcgaaa cagaactaga tgttgtagaa
    12001 ggtatgcagt ttgatcgtgg ttacttatca caatatatgg taacagataa cgacaaaatg
    12061 gaagctgttc tagaaaaccc atatatcttg atcacagaca aaaaaatctc taacatccaa
    12121 gatattctgc cattgttaga acaaatcttg caacaatcac gtccattgtt gattattgct
    12181 gacgatgtag atggtgaagc attgccaact cttgtattga acaaaatccg tggaacattc
    12241 aacgtagtag ctgtaaaagc tcctggtttc ggcgatcgtc gtaaagcaat gcttgaagat
    12301 atcgctattt tgacaggtgg tacagtaatt actgacgatc taggtttaga attgaaagat
    12361 gcaacaatcg aaaacttagg aaatgcttct aaagttgttg tagataaaga caatacaacg
    12421 atcgtagaag gttctggaga aaaagaagca atcgaagctc gtgtgcaatt gatcaaaaac
    12481 caaattgctg aaacaacttc tgattttgac cgcgaaaaat tacaagaacg cttagctaaa
    12541 ttagcaggtg gtgtagctgt tgttaaagtt ggtgcagcta ctgaaacaga attgaaagaa
    12601 ttaaaattac gcattgaaga tgcattgaat gctacacgtg ccgcagtaga agaaggaatg
    12661 gtctcaggtg gtggtacagc tctagtgaac gttatcagca aagtttctgc tgtagaagca
    12721 gaaggagacg tggcaacagg tatcaagatc gttgttcgtg ctttagaaga accaattcgt
    12781 caaatcgctg aaaacgctgg ttatgaagga tcagtgattg ttgataaatt gaagaacgtg
    12841 gaactaggca ctggtttcaa tgctgctact ggagaatggg taaacatggt tgaagcaggt
    12901 atcgtagacc caactaaagt aacacgttct gccttgcaaa atgccgcttc cgtttctgcc
    12961 ttgttattaa cgacagaagc agtcgttgca gataaaccag aaccagcagc gccagcagct
    13021 cctgcaatgg atccatcaat gatgggcggt atgatgtagt atttaataga gaaccttgat
    13081 atatcagggt tctctattct ttttttgttt tattttaaac gaaaggggca aagaaggggc
    13141 aaaaatatca aaggctattt agtttatcaa ttacttgttg tttggctctt ttggttacat
    13201 ggttataaat agacagagtt gtatttgcat cagaatgccc tacacgctcc ataatggctt
    13261 taagaggtac gcctaattca gataatagtg aaacgtggct atgtctgaat atatgtgagg
    13321 atagactttt ttctaattcc aactcttcct ctactttatg gagtatagcg ttaaatgagt
    13381 gcagcgtaag cggagtgcca cttgtagata taaatatata ttgatcggga tctgtgggtc
    13441 tacctgcaag aatattgtca gctatcacac tttcaatcaa ttcttttgca cgattgggta
    13501 attgcacttc acgttgcgaa taagtatttt ttggagttgt ttttatagca ttatccattt
    13561 tcacagatgt gtaatctaag gtcccattaa tggaaatttt cccatcctca tagtccttca
    13621 tttgcaaagc tagcaattcc ccatatctca aaccagtcaa atatagaaat tcagctatta
    13681 tgccgtgtag ttttctgcga ggattggaat ataactgttt cagtatttga tcaatttctt
    13741 ctttctccag atatttttta tccatagaaa gctttctttt ttcttcttcc acttttttag
    13801 gatggatttt aactgctaga gcagggtttc tttgaatgta ttttctatca attgcatagt
    13861 ttagcataac ggatagagtt gtttttgttt gctttgtgta gttcagtgag agatcaccaa
    13921 aagtgtacat atcttcaatc atcttattaa tgagtgtctc atcaatgttt ctaacgattg
    13981 tatcatcgct tatgtgctta gaaacatgtt tcatcatcat tggaaccttc aaatagctag
    14041 tacgtttaac atgctgctta taatattcat accattcttt atacagctca ccaaaagtga
    14101 tatctgattt attgtaattt tcgagtgctt ctttaatttt tttatctaga attttctgag
    14161 cttttttcca cgcttgtggt gaattacttg taagtgttgt agattttttt cgtgtttttt
    14221 ctgtataagg atctacatat ctttcaataa acttgaattg gccatctttg gtttgttcaa
    14281 cccacacttt taacatctcc tatcatttgc tataataggc ataacaaata gacctatata
    14341 ggtttgtttt ctaaaagcac gctcttactc tggacggtgg ggcgtgtttt ttatttatta
    14401 tacagttgct ttaaaaatag ctgctttttc aaatgatact aaaggagaga agtggatttc
    14461 tatttctccc ttttggttta acgtgaaatg agcagttaca tccatttgtt ttcctggagc
    14521 aacagatccc attgtatttt cgttggcata tgtctctgat tttttgtcat ctggtccata
    14581 tacttccaca tctgtaccta cagggatatc tgaatcgcca tcatttttta cggtataagt
    14641 aatttttact acttgtgcag gttggttttc ttcaaattga tttctttcat cagttagttc
    14701 tacactattt agtgtatatt cagcatcacc aacttttaca gtgtcaccaa tcttatagaa
    14761 ggtatcgctt ttttcttctt tagaagatga agcagaggtt gaagatttag ttactttttc
    14821 gccaccatta tcacttgctt tattactatc tgatccacca tttaaagcag aaccaataat
    14881 tataattaaa attactgcta atacccaaaa ccatactttt ttgtaaaaag gtttactaac
    14941 tttatacatt tttccgtctt gacccataac tttttttgcc atttaaatat tcctcacttc
    15001 ttgttataat atatttgcga tctcagaaat gaggtatgag tccgtgtgcc agcacgggct
    15061 tttttaatgt tttgctgaaa tcggtttttt taataattct tgatgtattc atacatattg
    15121 ccttgcgtaa gaatattttt tcttaaaata gcattggcag acagcataac aagtgtatct
    15181 gagcttattc tgaatagaat aatactccat aaatttttcg agattaaact gagattcatc
    15241 agtcagttcg ttctcaatat agatatttaa taaaattaga atagctattt tatctgcttc
    15301 tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac aaaactgaaa attcaaaatg
    15361 agaggcgctg aaatgtgcaa gttcatgaga taa
//
LOCUS       DAJSVD010000063        15286 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSVD010000063 DAJSVD010000000
VERSION     DAJSVD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.064"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ON498_001796"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ON498_001796"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ON498_001797"
     CDS             complement(580..1353)
                     /locus_tag="ON498_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3940044.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ON498_001798"
     CDS             1497..1847
                     /locus_tag="ON498_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3940045.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ON498_001799"
     CDS             1828..2544
                     /locus_tag="ON498_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3940046.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ON498_001800"
     CDS             2544..3992
                     /locus_tag="ON498_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3940047.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ON498_001801"
     CDS             complement(4062..4571)
                     /locus_tag="ON498_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940048.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ON498_001802"
     CDS             complement(4561..5286)
                     /locus_tag="ON498_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940049.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5509..7629)
                     /locus_tag="ON498_001803"
     CDS             complement(5509..7629)
                     /locus_tag="ON498_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3940050.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7859..9037
                     /locus_tag="ON498_001804"
     CDS             7859..9037
                     /locus_tag="ON498_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR3940051.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9053..9811
                     /gene="aroD"
                     /locus_tag="ON498_001805"
     CDS             9053..9811
                     /gene="aroD"
                     /locus_tag="ON498_001805"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3940052.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9834..10319
                     /gene="ybaK"
                     /locus_tag="ON498_001806"
     CDS             9834..10319
                     /gene="ybaK"
                     /locus_tag="ON498_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR3940053.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10390..11643)
                     /locus_tag="ON498_001807"
     CDS             complement(10390..11643)
                     /locus_tag="ON498_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3940054.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11760..13109
                     /gene="gdhA"
                     /locus_tag="ON498_001808"
     CDS             11760..13109
                     /gene="gdhA"
                     /locus_tag="ON498_001808"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3940055.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13221..14567
                     /locus_tag="ON498_001809"
     CDS             13221..14567
                     /locus_tag="ON498_001809"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3940056.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14875..15261)
                     /locus_tag="ON498_001810"
     CDS             complement(14875..15261)
                     /locus_tag="ON498_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3940057.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4990 a         2737 c         3104 g         4455 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
     5521 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
     5581 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
     5641 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
     5701 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
     5761 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
     5821 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
     5881 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
     5941 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
     6001 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
     6061 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
     6121 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
     6181 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
     6241 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
     6301 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
     6361 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     6421 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     6481 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     6541 gaatgtacca gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat
     6601 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     6661 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     6721 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     6781 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     6841 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     6901 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     6961 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     7021 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     7081 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     7141 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     7201 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     7261 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     7321 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     7381 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     7441 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     7501 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     7561 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     7621 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     7681 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     7741 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     7801 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     7861 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     7921 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     7981 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     8041 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     8101 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     8161 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     8221 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     8281 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     8341 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     8401 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     8461 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     8521 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     8581 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     8641 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     8701 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     8761 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     8821 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     8881 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     8941 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     9001 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     9061 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     9121 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     9181 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     9241 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     9301 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     9361 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     9421 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     9481 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     9541 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     9601 ggagttgact agacgggcaa atgctttttt ctccatgcct attattacga tgtccatggg
     9661 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     9721 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     9781 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     9841 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     9901 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     9961 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
    10021 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
    10081 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
    10141 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
    10201 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
    10261 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
    10321 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
    10381 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
    10441 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
    10501 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
    10561 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
    10621 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
    10681 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
    10741 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
    10801 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
    10861 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
    10921 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
    10981 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
    11041 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
    11101 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
    11161 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
    11221 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
    11281 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
    11341 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
    11401 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
    11461 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
    11521 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
    11581 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
    11641 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
    11701 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
    11761 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
    11821 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
    11881 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
    11941 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
    12001 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
    12061 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
    12121 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
    12181 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
    12241 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
    12301 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
    12361 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
    12421 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
    12481 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
    12541 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
    12601 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
    12661 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
    12721 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
    12781 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
    12841 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
    12901 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
    12961 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
    13021 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
    13081 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
    13141 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
    13201 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
    13261 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
    13321 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
    13381 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
    13441 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
    13501 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
    13561 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
    13621 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
    13681 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
    13741 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
    13801 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
    13861 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
    13921 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
    13981 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
    14041 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
    14101 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
    14161 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
    14221 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
    14281 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
    14341 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
    14401 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
    14461 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
    14521 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
    14581 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
    14641 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
    14701 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
    14761 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
    14821 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
    14881 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
    14941 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
    15001 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
    15061 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
    15121 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
    15181 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
    15241 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DAJSVD010000064        14870 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSVD010000064 DAJSVD010000000
VERSION     DAJSVD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.065"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..809
                     /locus_tag="ON498_001811"
     CDS             <1..809
                     /locus_tag="ON498_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3940058.1"
                     /translation="IIIFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKK
                     LADQLLEQQVRMQQDYIKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYD
                     QTILPTRQMMNLFPANLSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDT
                     KHTVNLVRMLGIILDNAIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLK
                     QKGFSTKTNPKNEGLGLFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            822..1550
                     /locus_tag="ON498_001812"
     CDS             822..1550
                     /locus_tag="ON498_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3940059.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(1619..3142)
                     /locus_tag="ON498_001813"
     CDS             complement(1619..3142)
                     /locus_tag="ON498_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3940060.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            3394..4575
                     /locus_tag="ON498_001814"
     CDS             3394..4575
                     /locus_tag="ON498_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3940061.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(4635..6263)
                     /gene="treC"
                     /locus_tag="ON498_001815"
     CDS             complement(4635..6263)
                     /gene="treC"
                     /locus_tag="ON498_001815"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3940062.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(6714..9425)
                     /locus_tag="ON498_001816"
     CDS             complement(6714..9425)
                     /locus_tag="ON498_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940063.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(9435..10136)
                     /locus_tag="ON498_001817"
     CDS             complement(9435..10136)
                     /locus_tag="ON498_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940064.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            10322..10921
                     /locus_tag="ON498_001818"
     CDS             10322..10921
                     /locus_tag="ON498_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3940065.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            11002..12015
                     /locus_tag="ON498_001819"
     CDS             11002..12015
                     /locus_tag="ON498_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3940066.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            12123..13040
                     /gene="rbsK"
                     /locus_tag="ON498_001820"
     CDS             12123..13040
                     /gene="rbsK"
                     /locus_tag="ON498_001820"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3940067.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            13068..13463
                     /gene="rbsD"
                     /locus_tag="ON498_001821"
     CDS             13068..13463
                     /gene="rbsD"
                     /locus_tag="ON498_001821"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3940068.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            13463..14836
                     /locus_tag="ON498_001822"
     CDS             13463..14836
                     /locus_tag="ON498_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3940069.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         4341 a         2925 c         2732 g         4872 t
ORIGIN      
        1 atatcattat tttttatacc cgtttttctg gtgaaacacc tgatgtctta gctttgaata
       61 cgagtttttt cattctatat ctgtgtgttg gcctgatcat cttggtttat tattggaaaa
      121 tctctaataa gaaattagct gatcagttat tagagcaaca agtcaggatg caacaggatt
      181 atatcaaaga tctagaaaaa aactatcaag acttgagaga attcaaacat gattaccaaa
      241 atctgctttt ttctcttcac agctatattt cagaaggcga cttagaagga ttacggtctt
      301 attatgatca aacgatatta cctacacgcc agatgatgaa cctttttccg gccaatctaa
      361 gtatgttgga taatatgaaa gttcctgaga tccgtagttt actgtcatta aaactcatga
      421 tggcacagga aaaagggttg atcgtccagc ttcattttcc tgagccaatc gaactggata
      481 cgaaacatac ggtaaacctt gtacgtatgc ttggtattat cttggacaat gcgattgaag
      541 gagctagtgc tgcaaagaag aaaaaaatcg aattgaccat actcaagaag aatcattccg
      601 tgatgattcg tgtgatcaat acaacaacaa atacattacc gttaaaccag ttgaaacaaa
      661 aagggttctc aacaaaaaca aatccaaaaa acgaaggcct tggcttgttt atcttggatg
      721 aattgacaaa gaccaatcct tatcttttcc ttgaaacatc aattgaaaac cagcgttttt
      781 ctcaaacgat ttatatacaa ataaaataaa ggagtaatga tatgattccc atttatattt
      841 gtgaagacga tccaaaacaa ttagaagttt tatcaacggt gatcaaaaat agaatcatga
      901 ttgaaaactt agacagttat gtccatattt ctacctctga tccggaagaa cttctcaatg
      961 cagccaagat cagaacttct tcttttggta tttacttttt agatatcgaa ctagaagaca
     1021 gtgaaatgga tggtgtagcg atcggtaaat tgatccgtga attagatcct ttaggaaaaa
     1081 taatctatgt gacttcccat acggaaatgt cgatgaaaat cttaaaaagc aacatcgaac
     1141 cgactgatta tatcgtcaaa gaagatcttt atgatttaaa agaaaatgtc gagcatatcc
     1201 tgagcaaaat tttcgaagat tttcaaatgt cccaaatcca aaaagatatc ttcaaaatag
     1261 agtttaatga tgagatcaaa tttttaccga tcaaagacat ccaatatttt tctacttcgc
     1321 taggagcgcc acataaactg gaagtccatt tgactcatgc ccaattacaa ttctatgaga
     1381 agatcaaaga aatcgagaaa atgcatcgga attttatccg ttgtcataaa tcctatgtta
     1441 tcaatacaca aaatgtgcgg tcgattaata aaaaaaccag agaagtcacg ttggcaaacg
     1501 gcgagaccat cccagcctct atccgtggat taaagaaatt gatttcataa aaagaaccga
     1561 atagtttgga ccgactaaat gaccagccga tccaaaccct tcggttcttt tttgcctatc
     1621 agtgcaaaag catttcctgt gcaatttcac gtccatccat tgtatctgga taaaatgttg
     1681 gccaattctc catttctttc agcaatactt cttgactttc atttcctgca tataaatgaa
     1741 aatgttcgga gtcagccggt gcaatcgtgt gatcactgaa ttgaatatat ctaaaagcgc
     1801 cactttcctt atcttctgct tcgaatagat accttacgcc acggtttcct gcctcgtagg
     1861 tcaagatttc tttccctaca taacgataat ttgaacgttt cgttgttcca ttcttaaacg
     1921 taaatgccat ctctttctca ctgatcttga ttgaagcaat atctgtttga tagcccttcg
     1981 tataatattc tttatattct tgtgctgtct tatcctgatt caatttttct ttatattcaa
     2041 aaacaggatc aaaagtccca tcttctacat acgtatctac tgtttgccaa gttcctgccc
     2101 aatctgaaag agagcgatct tgtacatcct cgtcttcaaa ataaccgttg tagacgctct
     2161 tttctttttt gggggtcaaa gtatcttcta gcgggttttc tgtctctgtc gtttttttca
     2221 acgcttcgag gtttgcttcc atgatagaaa gatagttttc tccattttct atttgttcat
     2281 tcgtcaatcc ttccaaagga ttcaatactt ctaaagatac acctgcttcg tttgctaacg
     2341 ttctggcaat actatctttg gcgttttctt caaaataaat atagttgatt ccatgttctt
     2401 taacaaagtg tttcagttca gcaattctcg ccgcagacgg ttcttcacta gaagaaagac
     2461 cagcaatcga tacttgattc aatccatatt ctaacgctag atagttgaat gcagcatgtt
     2521 gcgtgacaaa agatctttgt tttgcttgct taaaggtttc ttgataaagc tgatggagtg
     2581 cttgaagttt ctcggtatag gctgctgcat tttttgtcca tacttcctgt ttttctgggt
     2641 aagcttcaat cagctgatca cgtatgtttg ctacctgctt gattgctaaa cttggagcta
     2701 gccatacatg tgggtctagc tcatgtgtat ggccttcttc cccgtgttca tggtcatgat
     2761 catgtccttc ttcacttccc ggaagtaaaa gcataccttc tgtcgctttt atcgtatgga
     2821 cattgccttc ttgtaatgtt ttttggattg cggggaccca tgtttccatg ttttcatcat
     2881 tataaacaaa gatatctgta tcttggatct ttgccatgtc tttagcagaa ggttcataat
     2941 cgtgggaatc agtccctgct ggaatcaata actctacttc tccttcatcc ccgacgattg
     3001 cttttgtaaa atcatacatg ggataaaagg tcgtcatcac gctcaatttt tctttactgt
     3061 cctcggcttt cccacttgcc ccgcaagcag ttaacatacc tgctaaaacc agcatggcca
     3121 atatacctat caatcgtttc atttatgaca ctcctcttct catcttcttt catttgaatg
     3181 ttccgattcg cttttcgcaa accgtaatag ttacgtttta gcaacaggat ttaatttatc
     3241 atatggttaa aagaactgtc aagcgttttc ttattttaat tgatgagaaa gtttatatga
     3301 atgattgacg atatttttgc aatcaacctc tatcattttc cttttatacc cgcttttgtc
     3361 tataatgaat ttatagataa aggaggaaaa agagtgaaaa aagaaacaga tacaacgaat
     3421 cgtttgatcc gctttttagg aggaaaaacc tcttactatg ttttaggaat agttatctta
     3481 cttgcgctga tcatttattt tttccatcag atctcgttta tattcaagcc tgtcggaatc
     3541 attatcgcta ctatcttgcc cccattattg tttgcactga tcttgtatta tcttttggaa
     3601 ccagtcatca aacgtttgag caagcacctt tcgaggaact tgtcagtcat tatcgtttat
     3661 gtgattattt tatttttatt ggggcttgga agcatatggc ttattccttt tcttgaagaa
     3721 caggcaaaga cattgatcag tagtttgcca gatttgtttc aagattttca agagagtctc
     3781 cgacaatttt tggagaaaac accttttgcc gattcattta atcaattttt cgcttctatt
     3841 gacgacgtga cgtctgatat agctggattt attggaaatt attgggaaag tggcgcacaa
     3901 cggatcagca acattttttc aactgttaca gctattttta ttactttatt tactggtcca
     3961 attgtcgctt ttttcctttt gagagatcct caaaaatttt atcagtcagt tttagcgatt
     4021 gtcccaccag catttcgtac agattttaaa aatttgacaa agattgctaa tcaacaattg
     4081 ggttcatttc taaaggggca aatcattgct tcatttatcc ttggagcagt ctactgggtt
     4141 tgtttcttac tgatcggatt agaatttgct agtgttttag ctatttctgc tgggttgtta
     4201 tgtatcattc catatattgg gccgtttatc gccttctttc caggtttgat cattgcattc
     4261 caagactcta cttttatggc tgtcaaattt gtcattgtct ggtttgccgt acaacttctc
     4321 cacggtgact tggtgattcc tcgagtaatg ggtgatcgtt tacagatcca tccgattacg
     4381 atcttaatcg ttttacttgt catgggtgat ctaatgggaa ttgtgggcgt tatttttggt
     4441 attcctatct atacgttagt caaattactc gttactttta ttttccgtaa attcaaacaa
     4501 cgctacaata aattttatgg cgataaagga gagtatgaaa actcttcatt ttctgaagaa
     4561 gattatttta aataaaacac aaaacgtcga tcttacgaga atagctcgta ggaacgacgt
     4621 tttgtgtttt tgttttaatt tttcagcaaa gcaattgcct ggtatggttt tagctccaga
     4681 ctttgatgaa gaacagtatc ggaataatta tcgataagaa tattggcttc tgtgaatcct
     4741 tctgggatgg cgatccttgt atctttatca tagaaatttg ttaatacaag cagctgctgt
     4801 ccttcaaatt ggcgaagaaa gccataaact tccggatgat ttgcttcata agcatgataa
     4861 tctccttgtg caacgattgg atatgttttt ctcagccgaa tcaatttctt gtaataggac
     4921 aaaatagaat cttcagaaga tagttctttt tctacattaa tctcttctag cttgcctcca
     4981 acttttaacc aaggtttccc tgtagtaaat cctgcttgtt ctgtgtctga ccattgcatg
     5041 ggtgttcgag agttatcgcg agattttgct ttgatccgac gaaacgcttc tttttctgtg
     5101 aatccttgtt caaggagtaa cttataagca ttcaaacttt ctacgtcaat atagtcggaa
     5161 atcgatgaat agtctgggtc gatcatgccg atttcttctc ccatataaat atagggcgtc
     5221 ccacggttca aatggatcat ggttgctagc atctctgctc cttgttttcg atactgatcg
     5281 atagctacaa atcgattgag cgcacgtggc tgatcatgat tattaaaaaa tagagcattc
     5341 cagccgttac cctcactcat cttttctccc catgtatgaa atagtcgctt taattctgca
     5401 aaatcaaatg gaggagtaga ccatttattc ccattttcat aatcgacttt caaatgatgg
     5461 aaattaaaga ccattgataa ttcttctcta ctaggttctg tatacaagat acaattttcg
     5521 atcgtcgtag agctcatttc accgactgtg atgatagtgg aatccgtccc aaacgttgct
     5581 tggttcaatt cttttagata cgtatgggtg attggtttgt ccgtatattc aggtttgcca
     5641 tcattttctt tattattttt caattgttcg tctttaccga taacattgat aacatcgaaa
     5701 cggaatcctt ttaccccttt ttccatccaa aaacgaacaa ctttgaatag ttcctctctt
     5761 actgcttggt tccgccagtt caagtcagct tgtgtccgat catataagtg caaataatat
     5821 ttccccgtat ctccaaatgg cgaccaagca cttccaccaa attttgatac ccaatccgtt
     5881 ggctcttctc ttaaaatgaa gaagtcctga taatgctggt cccctgctag tgcttttttg
     5941 aaccattcat gttctgtcga aacgtgattc agcaccatat cgagcatgat ttctatccgg
     6001 tatttctttg cttcggctat tagctgttcg aattcctcca tcgtaccaaa gataggatcg
     6061 atggctgtgt aatcagagat atcataaccg ttgtcttttt gagggcttgg ataaataggg
     6121 tttagccaaa tcatgtcgat gcctagatca ttcagatacg ggagtttttc tttgattcct
     6181 tttaaatctc caattccatc accattactg tctttgtaag atttgggata gatttggtaa
     6241 atgactttgt ctttaaatgt catggatatt tacctccatt caactgattt attacctacc
     6301 acaacttata tatacaagtt atcatttatt attataactg aaccgaaagc gtttgtaaaa
     6361 tttatcttta tacattctaa gaggtctgtt gcccaataaa aaacaagaga tcagacatgt
     6421 atttgtctat cctcttgttt aaaatagcaa aatattcttg tttcttctat aaatggtgtt
     6481 caaaagcttt tatctacggc aatcagtctt ctcaaatgta aaacatgagg agctaatttt
     6541 atttgagtat tctttttgct cgatgcagga cggctttttt acaacctctg attcatggtg
     6601 ttcaatcagc tgtccttcgg tattagctca aaaaacggca aaatatgaaa aactattttg
     6661 ccgtttgttt ctctaatact caggtcaaac cgctgatttc acaacctctg tttttattca
     6721 ttcgatttca acgcatcaat catatcgata tgctttaatt tgaaatgggt aacaatcatc
     6781 acaataaccg taaatacgat agttaatcct gctgataaga cataagccca ccatgtgatc
     6841 actaatggaa agatgacatt ctccatagac gcttgttgca agatgaagtc agtcaatata
     6901 tatcctacac caaatcctcc gatgattcct aaaagagtaa agatgatatt ttctcgaaca
     6961 atgtacatcg tgacttcttt atcaaaaaat ccaaggactt ttattgtaga taattcgcgt
     7021 acacgctcgg aaatattgat attagttaga ttatacaaga cgataaaagc cagcagtcct
     7081 gataatacga caaagattaa gacaaccgag tctaagttcg ccattgattc ttcttgcgtt
     7141 tcgatttgtt tggacatgaa agacgtattt gtcacagcgt ctgtggcaag cagtttctcg
     7201 gaaagcattt cttcttcttt tgttgtcatc ttatctgtac gcagcaaaaa tgcattatcc
     7261 atcatcgatt gatcagtaac tgaagcataa tattcagggg acatataaat aaagctacct
     7321 agatagtttt tcacgatttc ttttacttcg actttccatt ctttttgatc ttcatcataa
     7381 agcgaaatat gatctcctgt agataagtca aatagttctg ctactttttg cgtaatagcg
     7441 attcctgttt taggcaagga gcgtgggttc tcttcaagat cagttaaatg gataaaacga
     7501 tccaaatcag ctgttttact tggtacgatc actgtcagac tttgcgaagc atgtcctttt
     7561 tttctcaatt ctacggtttg agtatgaata gctaggatct catttacttt ttcttgcttc
     7621 tgcagcatat ccgctgcttt ttgacgacta tcttccgtgg atgaatccaa tgtaacgatt
     7681 gcttgatagt caatgatcgg tccaaattgt ttggctgaaa cagaacttag agaatctttc
     7741 aatcccacgc ctgcaaccat cagcccggca catccagcaa ttccgataat cgccataagc
     7801 attcttgact tatatcggaa gagatttcgg taactgattt tttgattaaa gctaaggcgt
     7861 gaccacagcg gagtgatgta ttcaagaaaa atgcgtttcc ctggcttcgg tgctttcggt
     7921 tgaagcaatg cagcaggccg ttctcttaaa tctttgaata aaacgatcat tgcagcgcca
     7981 aatgcggcaa tgaagaatgc ggcagctgct tgtaaaatcg gcggccagtc ataaaaaata
     8041 agcgcactcc caatatcata ccgctcgtta gacagctcaa aaatgatcct tggcaaaaga
     8101 ttcgttccta atatcgttcc taaaacaata cctatccctg aggctagact tgcatagatt
     8161 gcatattttt ttgcgatttc aactttcgta taacctaacg cctttaatgt cccgatttcc
     8221 ctgcggtttt cttctaccat acgtgtcatt gtcgtaaagg tgatcaatgc ggcaataaag
     8281 aaaaagaaaa caggaaaaac attggcaatc gcagcgatac gatcagacag atcaccaaat
     8341 tcttgaaaac cgacattttc tgaacgttca ttgtataggt atgatggttt agttaaattt
     8401 tcaatttttt ctgcatttcg attcaattct tcctctgcag aggccagttc acgttctttc
     8461 tcaactaatt gcctttcttg ttcagcaagt tgagcttctt gttgagataa tgtttgaaca
     8521 gcgattgttc gttgcgcttc aggaagctgc tggatagttg ctgactgttg cgctaactgt
     8581 tttttagcat cttctaaccg tgttcttgct tgcgttgttt gtgctttgcc atccgctatt
     8641 ttttgctgtg ccggttctaa ttcttcgttg gccgattttt tcagctcttc taagcgttct
     8701 tcgggtcgat cttgtaaata cttttctgcc ttttcttgat tttgctccat tttatctttg
     8761 taagctttgc tgtacgtatt caaattcttt acgtttgtaa aatcaagata taagacagaa
     8821 taaatatctg ttttaaaaac ggattcaggt aaatagacaa aataatcgac gctaccattt
     8881 ccgacgttcg cgtatcctct ctccaaattg ttgatgaaca atggagaacg gacaaagccc
     8941 gtgatggtat actctttttc tttcaattca tctgaatcaa tctgataaat atcaccgatt
     9001 ttataccctt catcttccgc tttttcatcc aatacaagct ggtttggttt ttccgggagt
     9061 tttccatcag ttaattgtag cttgttctgc ttgttatttt tgttataaga aaatatttga
     9121 atcacttcgt ttttgccagt attggaataa tagatctgtt ttcctgtttc tacttttgca
     9181 tctgggatct tttctgcttg agcaatatct ttttgtgtca agccaccagt agaaacgatt
     9241 tgcaaatcag aaagtcctgc tgtaccaaca tagtgatcca tcgttttttg catgacaggt
     9301 ccagcagttt tgacacctac aaataaaagt gttcctagca tgacaataag gatgattgca
     9361 atgaatcgtc ctttagattg ccgaatttcg cgaaaacctg ctttaagata tgttcttgtt
     9421 ttcataaaat ccccctacca tacaatctca tcaatggatg aaggatgatc atttaattca
     9481 acagacctta ctttagcatc attaatgtga atgactctat cagcgatggg cgctaaagca
     9541 gaattatgtg taatgatcaa aacggtattg ccgtgttgtc tgctagcgtt ttgcaataat
     9601 ttcaatactt gcttgcccgt ttcaaaatcc agcgcgcctg tcggttcatc acataaaaga
     9661 agtttgggat ttttagctag tgcacgagca attgaaaccc tctgctgttc gcctcctgat
     9721 aattgggacg gaaagttatt caatcgatga gctaggccca cttgcctcaa tacctctaca
     9781 ggatcaagcg catcaggaga aacttccgtt gccaattcca cattttcttt tgctgttaga
     9841 ttaggaacga gattgtagaa ttggaaaacg aaccctacat ctgttcgtcg gtaagcagta
     9901 agctgtttat ctgaaaattg cgcaatatct gtgtcatcaa taatgatctg accttcatca
     9961 ggtgtatcca tcccacctaa aatattaaga atcgttgact ttcctgctcc acttggacca
    10021 agtataatca ctaattcgcc tttttcaatt gaaaaagaaa tatcgtcatt tgctgtaatg
    10081 gtcgtttctc ccattttgta tcgtttgtat tcatttttta cttcgacata cttcactctg
    10141 attcctccat tttcacgata tttctcgcta tatctatgca tcgaatgcgt tttctcacac
    10201 ttatttattt taccactgaa tttctctaga ctaaagaaag gaatccttta atttagttga
    10261 caaataaacg atttatgatt tacttacata aaaaacattg ttatttgaaa gaaggaacat
    10321 catgaatgat ttaaaggaaa atatgatttt tgatttatca aaaactgctt ttgtcgcgat
    10381 cgacatgcaa aaagagatcg taaataacgg tacactctct ccacacactg cagcatctat
    10441 tctgacaaac aatgatttgt tagtaagaac attgaaacgt accgctgcct tgaaagtttt
    10501 agtgaacgta gacatctcca ctttccctta tttgagtcaa caaactgata tgggtggaat
    10561 gactgcttct gtacctccag aattcactga tttacttttg aaggattctt tgaaagatac
    10621 agataatatg ttgactatca caaaatacaa tccaagtgct ttttttggta cttctcttga
    10681 tcttcaactt cgccggcgtg gaatcgaaac tatcatttta agcggtgtag cgacaacaaa
    10741 cggtgtatat gctactgcat tagatgcctt tcaacacggg taccatatcg tgcttgctga
    10801 agatgcttgc agtgatcgtg acaaagaatc ccatcaattg ttcataaaaa agatatttcc
    10861 gaaaactgca cgagtacgct caacgaaaca aataatcgaa gctatccaac aaagcaaata
    10921 agtactggca cacgtgattt tcatttactt ctttttgatt tcatgggaaa ataaccacag
    10981 gatttagata ggagtatgaa catggtgaaa aaaaagatca caatcaaaga agtcgctaaa
    11041 ctttccggcg tttcaatcac aactgtctcc ttgatattaa atggaaatac ggagaaattc
    11101 agtccgaaaa ctgttcagaa agttttagct gccaaagaag aattaggcta tcagcctgac
    11161 tattttgcta gacaaatgat taccaaggaa acgaaaacaa ttggtgtact cgttcctgat
    11221 atcacgaatc cattcttcag tacactgatg cgaggaattg aggatatctt gtataagcag
    11281 aatttcgtca cgatattatg taatgctgat tctgatcatc aaaaagaaat cgaatatcta
    11341 gccgagctca cacgtcgagg tgtggatgga ttcattatcg cgacttctgc tgtctctaca
    11401 gatgcaatca acgaaaattt gaaaaaacaa ggccggccct tcattgtttt ggatcagaaa
    11461 aaatctgaag gatttagtga tgccgtacga acagacgatt ttcgcggtgg ttacctagcg
    11521 ggcatgcact tgcttagtct aggacatcaa acgatcgccc ttgtctatcc tgaaaatcca
    11581 ccggaaaatg tccacgctcg gattgagggg tttaaatctg ctcttgatgt ttaccaaatc
    11641 cctcacgacc aactgatcct cttacctaca cagttctcaa aacaaggtgg ctaccagatc
    11701 acagcagaac ttcttgattc tgctgcaact ggtgtgtttg ctttaaatga tgaattagct
    11761 tttggattat atcgcggatt agaggaagct ggaaaaagca tccctgaaga ctacagcatt
    11821 attggctatg ataatataga tatgtgcgag tatatcaagc cgaaactgac aacgatcgca
    11881 caaccaatct tcgaattggg acaaacatct gccaagttat tgttagatcg gatccaattc
    11941 ccagaaaaag aatgggaaga aaaaagatta cctgtacgat tagaaaaaag attttcaact
    12001 gcacccttga aatagctttc aaggtgtgct atatttttat cgaactatta aaacgtttta
    12061 ctaaaacgtt ttaaaaaata attttcccat caaaataaat gagatagttg gaggtttgta
    12121 aaatgaacac ggttacagta attggaagta ttaatatgga cactaccctt cgtttaacca
    12181 acatgcccaa gccaggcgaa acgatgcatg cgcatgagat ttttcacgct ggcggaggta
    12241 aaggtgccaa ccaagcagtg gcagccaaac gttcaggcgc acggacatct tttattggag
    12301 gcgttggggc tgacagcgaa ggacaacagt tattagatct actgacaaaa gaaaacatcg
    12361 atacatctgg gatagctgaa atccaaggcg ccacaaccgg acaagcaatg attatggtag
    12421 acgcagcagg agaaaatagt atcttgatcc atgcaggggc aaataatgct ttccatgaac
    12481 aagaagtcct aaaaaacaag caactgatta caaacagtga ctttattatt gctcagtttg
    12541 aaagtagctt agatgcgacg atcctagctt tttctattgc aaaagacgca ggaaaaacaa
    12601 cgatcctgaa tcctgcacca gctcgtgaaa cgattcctac tgaactactt gaaaagacag
    12661 atatcattat accaaatgaa acagaaacag aaatcattac aggaatcaga gtgacagatc
    12721 ataacagttt ggtcgcagca gccgaaaaac tccatgaatt aggtattgga acagtaatca
    12781 ttacactagg cagtgccggc gctttctatc atacagaaaa agaacacggt atcgtccctg
    12841 cttttaaagt ggatgccgtt gacacaacag ccgcaggtga tacatttatt ggcgcattaa
    12901 gcagcacact tcagccagac cttagtaact tgaaagaagc gatcctttat ggaaatctag
    12961 cctcttctgt tgctgtgcag tcttatggtg cgcaaccttc tatcccttac cgcgaagctt
    13021 tagaacaatc agcagtctaa aattaacaat gagaggaaga aacaaacatg aaaaaaggaa
    13081 aagtaatcaa ttcagatatt tcacgagtaa tcgcacaaat ggggcacttt gataaattaa
    13141 gtatcgggga tgcaggcatg cctgtcccct ttggtactga aaaaatagat ttggctgtct
    13201 caaacggtct tccaagtttt ttatctgttt tagaaaacgt tttagaagaa cttgaggtac
    13261 aatcgattga attggcggaa gaaataaaga caatgaaccc ggaaatattg gaaaaaatca
    13321 aattattgtt accagataca ccgattagct ttatcccaca tgaagaaatg aaaaaagagt
    13381 tgaataactg tcatgcattt attcgtacag gcgaaatgac accttacgcc aatattatat
    13441 tagtgagtgg agtggtcttc taatggaaac gacaatgaat cctgaacaga tggtactgtc
    13501 cgagcagatt catatgctga ttgaacagtt gggaggaaaa gacaatattg attctgtcct
    13561 tccatgtgcg actcggcttc gtatcaaatt gaaaaaacag ccagtagcca atgcagaaaa
    13621 attaaaacag caaatccctt ccattaagta tttcatttta ctcgatagat atgcagaaat
    13681 aatcatggat cagggattgg ctcctgctta caaggaattg acgcgtcaaa tcgatcattc
    13741 agctccgaaa gaaaagctaa atccgatcga aaaactattt ggtttgatat cggaaatctt
    13801 tacgccgatc attccgctgt ttgccggttc tggtatatta aaaggtctgg ttgttttagc
    13861 gactacgatt ggctggctta gtgaaacaag cggaacttat catattttgt ctgctgcgtc
    13921 gaatgccgtc ttttattttc tgccaatctt tcttgctttt tctgctgcca aaacgtttaa
    13981 agtgaatgga tatattgcgg ctgtcattgc tgcagcactg attgaaccga atctgacctc
    14041 tttactttca gacagtggca aagcagctac tgattttctg gggattcctg tagtactgat
    14101 gcagtatacg tctactgtca tgccggcgat tttaggtatt ttcttttatt cttttgttga
    14161 aaaattcctc aaaaaatata ttcgcgaaaa tatgcaatta gtatttgtac ctttactttc
    14221 attagttatc acggttcctt tgacgttaat ggtattcggg cctgcgggtg tttaccttgg
    14281 cgaaggttta gctgccgcaa ttacttggat gatggatatt aacggaccga tcagtggcgc
    14341 aatcatcgct ggtggttgga atattttggt aatatttggt atccaatggg ctgtaaaccc
    14401 tgtcatgatc agtaatatct ctgcttatgg gttcgaccga atcgttccgt tgacaggagc
    14461 agccaatttt ggaatggctg gtgcagcttt aggtgtattt ctgcgaagta aacgttcaaa
    14521 aacccgttcg atctccggtt ctgcttttgc ttcgatttta cttgcgggag tgaccgaacc
    14581 aactgtttac ggaattgcga tcccattgaa aaaaccattt gttgctgctt gcattggtgc
    14641 agctgctggt ggagctgtaa tgggatttgc ccaagtgaaa gcgatcgctt ttgtatttgg
    14701 tagtctgacc acattgcctg cattcatttc cggtactttt ttctggtact tagcaggact
    14761 ggctgttagt ctagtcgtag caatgatcac tactctcgtt agcgggtttg atgaagacct
    14821 aatgtcttat gaatagttaa tgaaaaagac tgaagtatga gatgtttatc
//
LOCUS       DAJSVD010000065        14841 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSVD010000065 DAJSVD010000000
VERSION     DAJSVD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.066"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            364..1305
                     /locus_tag="ON498_001823"
     CDS             364..1305
                     /locus_tag="ON498_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-xylanase"
                     /protein_id="HCR3940070.1"
                     /translation="METINGITFGFMSQRGDWQDPMSRESLRMMKEELAASHVILPIT
                     VTQAHPQSTKIDWQSDNVLIDAEVKGMIAYAQGIGLKVILKPMVNVADGTWRAHINFF
                     DYDVPCEPTWSQWFDSYREYLNHYAKLAEETGCVMLVIGCELVNSDRREAQWRETISQ
                     IRQHYNGLLTYNCDKYQENNLTWWDAVDVISSSGYYPIDKWEQELDRIEQVVNHYQKP
                     FFFCEVGCPSREGSQYLPNDWSFSGEVSMIAQSRWFEKMFTACDKRNWIQGFGIWDWK
                     ARLYSREMALKDDDYGVYGKPAAAIIREFYKSTKQYI"
     gene            1428..2738
                     /locus_tag="ON498_001824"
     CDS             1428..2738
                     /locus_tag="ON498_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3940071.1"
                     /translation="MKKKMLGSMIVLGVAGLVLGACGNNKDSANDKDGGKTETITFIN
                     HKTDWETNGKWDEYMTKFNEKYPDIKVEIQTITDYAGQIKTRMNSEEYGDVLMIPGDI
                     KPEDYGNFFEPLGKKEELSKEYMGLNDRSYDGTSYGLPSQMNATGMVVNQKVFKDAGI
                     DEFPQTPEDFVAALKTIKEKNTDVMPLYTNYAAGWTLSNWDFVRSGASGDKDFTNKMT
                     SDKEPFAAGKVMNTIYKTLYDVSNQGLIEADPTTSDWEQSKVDMANGKIGIMVLGSWA
                     VPQIKDINADTAEDIIFQPFPMTAEDGKQYLPIGADYNYGINVHSKHKEAARKLIDWL
                     VNESDYAEENGGLSAKIGADYPESLKAAQDAGVELIEENPAPAGKESLFSSINDQSEL
                     GLGTTDAEKQRIIDAAVGNSNESFEAIMKDFNTRWSDTIAKVGE"
     gene            2958..3875
                     /locus_tag="ON498_001825"
     CDS             2958..3875
                     /locus_tag="ON498_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3940072.1"
                     /translation="MNKQGEFTMNKTVKKSRFSQKTEKRILIFTFTIIPVFLLLMLSY
                     YPLVKMFQYSLTEWNGYSPDSKFVGLDNYKTVLTNPKYFTVFKTSLYYFFATFLQLGV
                     ALLFATILSFKVKFANFWKGILFFPYLLNGVAIGFIFLYFFKGDGTLDTVLSALGLES
                     QIRLWLGDRSINNISLAFTSLWRYTGFNFLIFLGAIQSVNPEVYEAAEIDGANRWDEF
                     RYIIVPSIRKIIFLNLILGISGSLSVFDIPYIMTGGSNGTMTFVIQTIDTAFKYNKVG
                     LASAMAVILLVIVLVVTLIQRKFISEKEA"
     gene            3879..4703
                     /locus_tag="ON498_001826"
     CDS             3879..4703
                     /locus_tag="ON498_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3940073.1"
                     /translation="MKRQFSIGRILQYLILIIGAVVAVLPILVVFIGSFKSNNEFLST
                     SVLSLPKSLDFSNYKTAFINGQMLTGFKNTLLIFSISMVGKLLLGSMFAYVMSRFDFK
                     LKKPIMALFMMAMLIPGITSQVATFQIIEGIGLFNTIWSVILLNLGTDVISIYVFLQY
                     LDEIPVSLDESAYLDGANYFGIFWRIILPNLKAPIVTMLIISGVGVYNDFYNPFLYMP
                     DRKLKVISTALFAFKGPYGTNWPVILAGVIIVILPILVVFLALQKYIYNGVAGSVK"
     gene            4729..5334
                     /locus_tag="ON498_001827"
     CDS             4729..5334
                     /locus_tag="ON498_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940074.1"
                     /translation="MTEKSSKLVVAVELLYVLVKCSLHFWGWLVTGGFIYGWVSSHKK
                     LVFCVDHPEALQEKLCRLTKKLPPTKLCEKVLSVGVFLSLAIFLSGAWLGSTSLGLFF
                     KVTGGLSLLLFLLYNAHWVLGTATYDEMKEAPVAIGYQLLLQTLRPSLLNGFILGTYA
                     FWILLLLVSPLLFFLVAPGGVAYLLKKGSQKFREMEGINHD"
     gene            5327..7549
                     /locus_tag="ON498_001828"
     CDS             5327..7549
                     /locus_tag="ON498_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3940075.1"
                     /translation="MISVNPERREFHLKNQNISYIFRVMEELDVLEQLYFGSSIPIYD
                     QYDFLIEREIRPANNQIKQNYTTSLEHIKQEVGLYGTTDFRYPMLRVRYPKGDRISHF
                     TYQHYRIELGKQALSGLPGSFGEAGETLIITLQDRYSPLQVELYYTLFGEESVVTRQM
                     KIINPSETTFELLDCLSFNLDLPNQHYDWLHLDGAWARETQISRNSIAYGVQEVASTR
                     GASSHVHNPFMAVCESNATEEQGKVYGFSLIYSGNFVARLQLDTYDILRIQMGINPFE
                     FAWQLTPKSTFLTPEAVLVYSEAGFNGMSQSFHHFFQEHLISPRWSHKQRPVLINNWE
                     ATYFDFDSEKLLQIAEKAAAIGVDLFVLDDGWFGTRNSDNGSLGNWQVQLAKIPEGIS
                     AFATKINQLGLEFGLWFEPEMISTDTPLYEMHPEWVIGNSEKQISHGRNQFVLDFSNP
                     EVVETIFQQIDCVLACGKITYLKWDMNRYISEPFSNYLADGNQGELFHRYILGVYDLY
                     EKILAKYPDLLIESCAGGGGRFDPGLLYYAPQTWASDDTDGVERLKIQYGASMVYPLA
                     SIGSHLSQIPNHQVGRSPGLTFRNQVAMFGTYGLELDITRMSDVELQQVKAAIATFKE
                     YRELIHEGRFYRLNSPFDNTQCSWMVVSKDLSEALVADYQVLGKPNPAYRRLLLAGLD
                     LEANYQINQLPQLRSGKDLSSIGLIMGGNYVGRAKDYWSRQLPGDYASQLYHLRRVDK
                     "
     gene            7637..8350
                     /locus_tag="ON498_001829"
     CDS             7637..8350
                     /locus_tag="ON498_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3940076.1"
                     /translation="MKKYILISSDIRKKILEGVYQANQQIPFEKDLCVEYDASKMTVK
                     KALDMLVSEGLIIKRRGSGTFVKDLATDEIERIAVANQFRGTTALNPGKEVKSKVLEF
                     KVIEADNLVKNKLNMQEDTFVYDIYRVRHIDGKPLVMEKMYMPIDLIPGLKKANIEGS
                     IYEYIEEDLGLKIQSGHRRVSVRKATEIEQQYLELDSGDPVAVAEQIAYFDTGIAFEY
                     SISVHRYDSFSVEFVLTRE"
     gene            8481..9365
                     /locus_tag="ON498_001830"
     CDS             8481..9365
                     /locus_tag="ON498_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940077.1"
                     /translation="MSILAIDIGGTSIKYALWAAEALTEKGSFVTPVTWEQMKMDLKA
                     TLHNLTSCSQTPIEAVSISVPGAVDSDQGIIGGYSAIPYIHKIHFVAELEELLEKPVF
                     VENDANCAGLAEAFYGAAKDAKSSVSFIIGSGIGGAVVLERKLVKGHNLFGGEFGYML
                     AENGQTLSELASPVHAAKRYCEQKGLVSGITGRELFDIADTGDQLAQQEVQQLFRALA
                     IGIYNVCLVIDPEVVCIGGGISMRKDLLAPVKAQLCLLQQHHHSEDQRITVKICQFQQ
                     DANLIGAVANYHLRKEQV"
     gene            9365..10342
                     /locus_tag="ON498_001831"
     CDS             9365..10342
                     /locus_tag="ON498_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HCR3940078.1"
                     /translation="MYAIDAKKRWQNYQGSQRKPADFEAFWQAGFQEVDDLGTDYHLE
                     PHPLNSSVAEAFDLTFTGVQGATVHCQLIRPKRLQKKSPLLLQFHGYHSDAGDWSDKV
                     AMAAEGFWVVALDVRGQGGLSEDVGLTTKTSLKGHIIRGVESGPNDLFYRSVFLDIYQ
                     LTRLMVAEPQVDTSQLYTYGASQGGALALICAAVAPEIKESFVCYPFLSDYREAYRLD
                     VENSAYEELAYWFRFKDPLHQREEAFFETLDYIDLQYFAPWIKGKVHWAIGLADTVCP
                     PKTQFAVYNQLSSAADKEMLFYPEYGHEYLPKFGDHMRQCLADVLREKE"
     gene            10349..11737
                     /locus_tag="ON498_001832"
     CDS             10349..11737
                     /locus_tag="ON498_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3940079.1"
                     /translation="MLTFPKDFWWGAATSGPQSEGRFQKKHANMFDHWYDIEPEVFYD
                     RVGPDMASNFYNSYREDIALMKQTGLNSLRTSIQWTRLIKDLETNEVDPVGVAFYNNV
                     IDEFLSQGIRPIFNLHHFDLPVELYHKYGGWESKKVVDLFVGFAEQCFALFGDRVKDW
                     VTHNEPMVVVDGEYLFQFHYPKLVDGKKAVQVAYNLNLASAKVMAKFREMGQPQAGGR
                     IGTVLNLTPTYAASDAEADQEAARIAELWNNKMFLDPAIKGEFPVELLELLTKDGVLW
                     QSTADELALIKENTVDFIGVNFYHPNRVKTPDIAPNSVGAWMPDRYYDAYQMPGRRMN
                     IDKGWEIYPKAVYDIAINIRDNYGNLPWFVSENGMGVSREERFMDEKGQILDDYRIEF
                     IKEHLQWLHKGIEEGSNCFGYHLWTPIDCWSWSNAYRNRYGLIANDIHTQIKTIKKSG
                     EWFKTLAENNGF"
     gene            12291..12737
                     /locus_tag="ON498_001833"
     CDS             12291..12737
                     /locus_tag="ON498_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3940080.1"
                     /translation="MSDYKKIYETTATNTGGRDGVSYLNDGSFEVKISTPKEMGGSGS
                     GVNPEQLFALGYSACFHGALEIVKGQRKIHQSSQVTHTVKLYKKPDDVDFKLSAEIQV
                     AIQDLDLEEVQKIAEEAHKVCPYSKAVKDSIEVSVRAVLYDADKEK"
     gene            12823..13512
                     /locus_tag="ON498_001834"
     CDS             12823..13512
                     /locus_tag="ON498_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3940081.1"
                     /translation="MIDFTKYDHCISTMPLFQGLSLTEIQFIQSDITEQAFSAGQFIY
                     QAEDILESLYLIQEGKVKVYRLGSSGKEQVIRILEQGDFVGELALFEEQHYGCFIEAI
                     SPTKVCSIQKDNFKRILLSHPQIALELLSEMARRLDNTERLTAGITTERAETRLIQYL
                     LEENIKQNTEQIKLETTKKELASYLGMTPETLSRYLTKLTRNNLIKQPIPNIIELTNV
                     TELKNLMDKVR"
     gene            complement(13557..13748)
                     /locus_tag="ON498_001835"
     CDS             complement(13557..13748)
                     /locus_tag="ON498_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940082.1"
                     /translation="MNTLKEDFILAKAGNEEAIEAILKRFSSLIHKKSWRNGKYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            complement(13824..14210)
                     /locus_tag="ON498_001836"
     CDS             complement(13824..14210)
                     /locus_tag="ON498_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3940083.1"
                     /translation="MSENDERVNAQLIRFFEKVIENTANSYFKKQKKISDHEQFDQFP
                     QHFFTENKIKIKQPVTILNVTFLVEDAQLAEIIPLLKEKEQIFLVEKFIFEKTDKEIG
                     ESLGITRQGTTNLKHRLYKKFQELLI"
     gene            complement(14269..14730)
                     /locus_tag="ON498_001837"
     CDS             complement(14269..14730)
                     /locus_tag="ON498_001837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940084.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRNLSKDYYELKSRLFTLDSMESH
                     ENSNKNKDFARKSSATISKEAQHQLYISERTNKNKQSIAYSTISLTIASILKGAGRPL
                     SNKEIFKILTNDHGLSISYENLTHNILPRINIDSSINVERAYRGYWQYRLH"
BASE COUNT         4503 a         2503 c         3279 g         4556 t
ORIGIN      
        1 cattgtcaag gacaatcgga atagcatacg gaaagaagtt ctgcacctta taaaatttgt
       61 caaaaaaact gttgccattc catcaagcag ctcttattag tagtgggatc ttatcccgac
      121 gaccgtaaaa accatcatga attagatcca tcttaccttg ccagaagggg atgatttttt
      181 gagtgagctg cgtgtacaat tttttcttat ccaaaaaaga cctccaattt tattatattt
      241 actatatcat attgtataaa tcattataat caaaagtata tatttttgtt gtttttttct
      301 tcttcgtcct ttataatata gaattgaaat cgattacaaa ataaaacgtg gagaagtgaa
      361 aagatggaaa ctatcaatgg gataactttt gggtttatga gccaacgggg agactggcaa
      421 gatccaatga gtcgagaatc attacgcatg atgaaagaag agttagctgc aagtcatgtg
      481 atattaccaa ttactgtcac acaagctcat cctcagtcta ccaaaatcga ttggcaaagt
      541 gacaacgttt taatagatgc agaagtgaaa gggatgatcg cttacgctca gggaatcggg
      601 ttgaaagtaa tcttgaaacc catggtcaat gtggcagatg gtacgtggcg agcgcacatc
      661 aatttttttg attacgatgt tccctgcgag ccgacttgga gccaatggtt tgattcctat
      721 cgtgaatacc tgaatcacta tgcaaaactc gctgaagaga cagggtgtgt catgttggta
      781 atcggctgtg aattggttaa tagtgatcga cgggaagctc agtggcggga aactatttcc
      841 cagattcgac agcattacaa tggtctacta acttataatt gtgacaagta tcaagaaaat
      901 aatctaactt ggtgggatgc tgtagatgtc atctcttcat caggctatta tccgattgac
      961 aaatgggaac aagaattgga tcggattgaa caagtggtaa atcactacca aaaacctttc
     1021 ttcttttgtg aagtgggttg tccaagtcgt gaaggaagtc aatatctacc caatgattgg
     1081 agtttttcag gggaagttag catgatagcg caatcacgat ggtttgaaaa gatgtttact
     1141 gcttgtgaca agagaaactg gattcaaggc tttggtattt gggactggaa ggcgcggtta
     1201 tattcgcgag agatggcgct caaagacgac gattacgggg tttacggtaa accggctgcg
     1261 gcgatcattc gagaatttta caaaagtaca aaacaatata tataaaagta tatactttta
     1321 aatgggaatg cagtattata tttatgtaat cgttttccat ttctgacgta acaggccatg
     1381 aacagttttt ggtaacagca ctttattaat ttataggagg aagtaacatg aagaaaaaaa
     1441 tgctaggttc aatgatcgtc ttgggagtag caggtttagt tctaggggca tgtggaaaca
     1501 acaaagattc agctaatgac aaggatggtg ggaaaactga gaccattacc ttcatcaacc
     1561 acaaaaccga ctgggaaacc aatggaaaat gggatgaata catgacaaaa ttcaatgaga
     1621 agtacccgga tatcaaagta gaaattcaaa cgattacaga ctatgctggc caaattaaaa
     1681 cccggatgaa tagtgaagaa tatggggatg ttttgatgat tccaggagat attaagccag
     1741 aagattatgg gaactttttt gaaccacttg gtaaaaagga agagctcagc aaagagtata
     1801 tgggtttaaa tgatcggtcc tatgatggca cgagttatgg cttaccgtca cagatgaacg
     1861 ccactgggat ggtggtcaat caaaaggtct tcaaagatgc tggtattgac gaatttccac
     1921 aaacaccaga agattttgta gcagcattaa aaacaatcaa agaaaaaaat actgatgtga
     1981 tgccgctata tactaactat gcggctggtt ggacattatc aaactgggac tttgttcgca
     2041 gtggtgcttc tggtgataaa gactttacca ataagatgac ttcagacaaa gagccatttg
     2101 cagcgggtaa agtgatgaat acgatttata aaaccctata cgatgttagc aatcaagggt
     2161 tgattgaggc tgacccaact acatctgatt gggaacaatc aaaagtagat atggctaatg
     2221 gtaagattgg gatcatggtt ttgggaagtt gggcggtacc acaaatcaaa gacatcaatg
     2281 ctgatactgc tgaggacatt atcttccaac catttccaat gactgccgag gatggtaaac
     2341 aatatttgcc aattggagca gattataact atgggatcaa cgttcatagt aagcacaaag
     2401 aagcggcgcg caagttgatc gactggttag tcaatgaatc agactacgcg gaagaaaacg
     2461 ggggcttgtc agcaaaaatt ggagcagatt atccagaatc attgaaggct gcacaagatg
     2521 ctggggttga gttaattgaa gaaaatccag caccagccgg aaaagaatca ttgtttagtt
     2581 caatcaatga ccaatctgaa ctaggtttgg gtacgacaga tgctgaaaag caacgaatca
     2641 tcgatgcagc agttggtaac tctaacgaaa gcttcgaagc tattatgaaa gacttcaata
     2701 cacgctggtc agatacgata gcaaaagtgg gagagtaaaa tttttttaga agtcacccta
     2761 gcagttagga aaagactttt gattgatagc aaataaagtt ttgattgtag gcactaactt
     2821 ttgtctggca acttgtgcca ggcaaaagca cagtgtcgta aagaaaactt atcttggtag
     2881 taacttcttt tgcaggagta catagcgatt gcggaccgtg gtcgatatga ggggttttag
     2941 aataaggagg agccaacatg aataaacagg gagagtttac catgaataag acggtgaaaa
     3001 aatcccgttt tagccaaaaa actgaaaaaa gaatcttgat ctttactttt accattattc
     3061 cggtattttt attattaatg ttatcttatt atcctttagt gaagatgttc cagtacagtt
     3121 tgacagagtg gaatggttat tccccagaca gtaaatttgt cggactagat aactacaaga
     3181 cagtcttaac caatccaaaa tactttaccg tttttaaaac cagcttgtat tacttctttg
     3241 caactttttt gcaattgggt gtcgctttat tgtttgcaac gattttgtcc tttaaggtca
     3301 aatttgctaa tttttggaag gggattttgt ttttccctta tttactaaat ggggtagcaa
     3361 ttggatttat tttcttgtac ttctttaaag gtgatggaac tttggataca gtgctatcgg
     3421 cgctaggttt ggagagtcaa attcggctat ggttaggaga tcgctccatc aataatattt
     3481 ctttggcctt tacttccttg tggcgttata ccggctttaa ctttttgatt tttttaggag
     3541 caatccagtc ggtcaatcca gaagtttatg aagcagccga aatagatggg gctaatcgtt
     3601 gggatgagtt tcgttatatc atcgtaccga gtattcgcaa aatcatcttc ctaaatctga
     3661 ttttgggcat aagcggctcc ttgagtgtct ttgatattcc atatattatg actggaggat
     3721 ccaacggcac catgaccttt gtaatccaaa ccatcgatac tgcctttaag tataataaag
     3781 ttggtttagc ttcagcgatg gcagttattt tactcgtgat tgtcttagtg gtgacgttga
     3841 ttcaacggaa atttattagc gagaaggagg catagaagat gaaaagacaa ttttctatcg
     3901 gacgtatact tcaatatttg attttgatta tcggggcagt ggttgccgta ctaccaatcc
     3961 tggtagtttt tattggctct ttcaaatcca ataatgaatt tttgtctacc agtgtcttgt
     4021 cgctaccaaa gtcattggac tttagtaatt acaaaactgc gtttatcaat ggtcagatgt
     4081 tgacaggttt taaaaatacg ttgcttattt tttcaattag tatggtaggt aagcttttgt
     4141 taggctcgat gtttgcctat gttatgagcc gttttgattt taaactgaaa aagccgatta
     4201 tggctttgtt tatgatggca atgttgattc ctggcattac tagtcaagta gcgacgttcc
     4261 aaatcattga aggaattggc ttgttcaaca cgatttggtc agttatttta ctaaatttgg
     4321 gaacagatgt gatttccatc tatgtttttt tgcaatactt agatgaaatc ccagtctctc
     4381 tagacgaatc ggcctatttg gacggggcca actattttgg tatcttttgg cggattattt
     4441 taccgaactt aaaggcacct attgtcacta tgctaatcat cagcggtgtg ggtgtttaca
     4501 atgatttcta taatccattc ttgtatatgc ccgatcgtaa actaaaagtg atctccacgg
     4561 cactctttgc attcaaaggc ccttatggaa ccaactggcc agtgatctta gccggggtaa
     4621 ttattgttat tttaccgatt ttggttgtct tcttggcttt gcaaaaatac atttacaatg
     4681 gtgttgctgg ttctgtgaaa taagcttacc ggaaaaggag ggttactggt gaccgagaag
     4741 tccagtaaat tagtagttgc agtggaatta ctctatgtcc tagtaaagtg ttccttgcat
     4801 ttttggggct ggttagtcac tggtggcttt atttatggct gggttagtag ccataaaaag
     4861 ttagtttttt gtgtcgacca tccagaagcc ttacaagaaa agctctgtcg actaaccaag
     4921 aagctaccac ctacgaaact gtgcgaaaaa gtgctatctg tgggcgtatt tttgtccttg
     4981 gcgatctttt tatctggtgc gtggctcggt agcacttcac taggcttgtt ttttaaagtt
     5041 actgggggat tatctttgct tcttttctta ctctataatg cacactgggt cttgggaaca
     5101 gcgacgtatg acgaaatgaa ggaagctcct gttgctattg ggtatcagct attacttcag
     5161 acattacgac ccagtttgct aaatggcttc atcttgggga cctatgcctt ttggatccta
     5221 ttattgctgg tcagtccctt gctgtttttc ttggtggctc ctggcggagt ggcctattta
     5281 ctgaaaaaag gctcgcaaaa atttcgtgaa atggaaggaa taaatcatga ttagtgtcaa
     5341 tcctgaaaga agagagtttc acctaaaaaa tcagaacatt agttatattt ttcgggtaat
     5401 ggaagagtta gacgtcttgg aacaactcta ttttggaagt agtattccta tttatgatca
     5461 atatgatttt ttgattgaaa gagaaatacg tcccgctaac aaccaaatca agcaaaacta
     5521 cactacttcg ttggaacata tcaaacaaga agtgggcctc tatggtacca ctgattttcg
     5581 ctatcccatg ctacgggtac gctatccaaa aggagatcgt attagtcact ttacctatca
     5641 acactatcgg attgaattag gaaaacaggc actttctggc ttgcctggca gttttggtga
     5701 agcgggggag actttaatta tcaccttgca agatcgttat tcacctttgc aagtcgagct
     5761 ttactatacc ttgtttggtg aagaatctgt agtgaccaga caaatgaaga tcatcaatcc
     5821 tagtgagacg acctttgagt tattggactg tctgagtttt aatctggatc ttccaaatca
     5881 gcactatgat tggctacact tggatggagc ttgggcgaga gaaacccaga ttagtagaaa
     5941 ttccattgct tatggtgtgc aagaagtcgc cagtactcgt ggagccagca gtcatgtcca
     6001 caatcctttt atggctgttt gtgagtccaa tgcgacggaa gaacaaggaa aggtctatgg
     6061 ctttagctta atttatagtg gcaattttgt ggcacgtttg cagctggata cttatgacat
     6121 tttgcgaatt caaatgggta tcaatccttt tgaatttgct tggcaattaa ctccaaaaag
     6181 cacatttttg acaccggaag cagtactggt gtatagtgag gccggtttta atggtatgag
     6241 tcaaagcttc catcattttt tccaagagca cttgatatca cctcgctggt ctcataagca
     6301 acgacctgtt ttaatcaaca attgggaagc cacttatttt gattttgaca gtgagaaatt
     6361 attacaaatc gctgaaaaag cagcagcaat cggtgtggat ctgtttgtgt tggatgacgg
     6421 ctggtttggt actcggaata gtgataacgg ctctttaggt aattggcagg tgcagctagc
     6481 caaaatacca gaggggatct ctgcctttgc gacgaaaatc aatcaattag gtttggaatt
     6541 tgggctgtgg tttgaacccg aaatgatttc tacggataca cccttgtatg agatgcaccc
     6601 tgagtgggta attggtaatt ctgagaagca aatctctcat ggacggaatc agtttgtctt
     6661 ggattttagc aacccagaag tggtggagac aattttccag caaatcgatt gcgttcttgc
     6721 ttgtggcaaa attacatacc tgaaatggga tatgaatcgt tatatttcag aaccattttc
     6781 caattacctc gccgatggta atcaaggaga gttgttccat cgctacattt taggtgttta
     6841 tgacctgtat gaaaaaatct tagcaaaata ccccgatttg ttgattgaat cctgcgctgg
     6901 tggcggtggc cgttttgatc ctgggttact ctactatgca ccccagactt gggccagtga
     6961 tgatactgat ggagtcgaac gcttgaaaat tcaatacggg gcttcgatgg tttacccatt
     7021 agctagtatc ggtagtcatt tgtcacagat tcccaatcat caagtagggc gttcgccagg
     7081 actgactttt cgtaatcagg tagcgatgtt tggtacttat ggtttggagt tggatattac
     7141 gaggatgtca gatgtagagc tacaacaggt aaaagcagcg attgccacct ttaaagaata
     7201 ccgagaattg atccacgagg ggcgttttta tcgtctcaat agtccttttg ataataccca
     7261 gtgtagttgg atggtagttt caaaagattt gagtgaagct ttggtggcgg attatcaagt
     7321 gttgggtaaa ccgaatccgg cttatcgtcg tttattattg gccggtttag atttggaagc
     7381 taattatcaa atcaatcaac tgccacaatt acgttctggt aaggatcttt ctagcattgg
     7441 cttgattatg ggtggcaact atgtcggtcg agcaaaagac tattggtccc gtcaattgcc
     7501 gggggattat gcgtctcaac tgtatcattt acgacgagta gataaataat ttcaaaagga
     7561 cagtggctgg ctatttccat tcggtagcag ataggataag atattaggat aagccatacg
     7621 tggaggtaac atgacgatga aaaaatatat ccttatttcc agtgatatcc gtaagaagat
     7681 actggaaggt gtatatcaag caaatcaaca aattcccttc gaaaaagatc tttgtgtgga
     7741 atatgacgct agtaagatga cggtgaaaaa ggccttagat atgttagtgt cagagggatt
     7801 gattatcaaa cgtcgtggat ccggtacttt tgtcaaggat ttggcgaccg atgaaataga
     7861 acgaattgcg gtggctaatc agtttcgtgg gacgactgct ctcaatccag gaaaagaagt
     7921 taaaagtaaa gttttggagt ttaaggtaat agaagcggat aacttggtaa aaaataaatt
     7981 aaatatgcag gaagatactt tcgtgtatga tatttatcgg gtgcgtcaca ttgatggcaa
     8041 gccgttggtt atggaaaaaa tgtacatgcc tatcgatttg attcctggtt tgaaaaaagc
     8101 caatattgaa gggtccattt atgaatatat tgaagaagat ctcggtttga aaatccaaag
     8161 tgggcatcga cgcgtcagtg tccgaaaggc gactgaaatc gaacaacagt atctagaatt
     8221 ggattcggga gatccggtgg cagtggcaga gcaaattgct tattttgata caggcatcgc
     8281 ttttgagtac tccatttcag ttcatcgtta tgatagtttt tcggtggagt ttgtcttaac
     8341 gagagaataa gtaagcggaa aaatcctctt tccgcttttt attttgattt tcaaatttta
     8401 aaagtatata cctaaaaata aataactact tacaatgaaa attagtgtag gtaagtagac
     8461 tgagaaagta gggggaattc ttgagtattt tagcaattga tattgggggt acgagtatca
     8521 aatatgctct gtgggcagca gaagccttga ccgaaaaagg ttcctttgtg acacccgtga
     8581 cttgggagca aatgaagatg gacctaaaag ccactttgca taatttaacc agttgtagtc
     8641 agacaccgat tgaagcggtg tcgatcagtg tacccggagc agtggacagt gatcagggga
     8701 ttatcggggg ctacagtgcg atcccctata ttcataaaat tcattttgtt gcagaattag
     8761 aggagttatt ggaaaaacct gtctttgtgg aaaatgatgc caattgtgca gggttagcag
     8821 aagcctttta tggtgctgcc aaagatgcca aaagttcagt ttctttcatc attggatcgg
     8881 gaatcggtgg agccgtggtg ttggagcgga aactagtcaa ggggcataac ttatttggag
     8941 gcgaatttgg ctacatgttg gcggaaaatg gacaaacgtt aagtgagtta gccagccctg
     9001 ttcatgctgc aaagcgctat tgcgaacaga aaggtttggt atcaggtatt acaggtcgag
     9061 aactatttga tattgctgac actggagatc aattggctca acaagaagtt caacaattgt
     9121 tccgagcctt ggcgattggt atttataatg tttgcttggt catcgatcct gaagtagtct
     9181 gtatcggtgg tggcatatca atgcgcaaag atctattggc tccggtaaaa gcccaattat
     9241 gtttgttaca acaacatcat cattcagagg accaacgaat tactgtgaag atttgtcagt
     9301 ttcaacaaga tgctaatctg ataggagcag tggcaaacta tcacttacga aaggagcaag
     9361 tttaatgtat gcaatagatg caaaaaaaag gtggcaaaac tatcaaggga gtcagcgaaa
     9421 gcctgctgat tttgaagctt tttggcaggc agggtttcaa gaggtagatg atttggggac
     9481 agactatcat ttagagcctc atcccttgaa ttcttcagta gcagaagcat ttgatttaac
     9541 ttttacaggt gttcaaggag ccaccgtcca ttgtcaactc attcggccta aacgactgca
     9601 aaaaaagtct cccctcttgc ttcaatttca tgggtatcac tcagatgctg gggattggtc
     9661 agacaaggtc gctatggctg ctgaaggctt ttgggtagtg gcactcgatg ttagaggaca
     9721 aggtggtttg tcagaagatg tgggattgac cacgaaaacc agtctgaaag ggcatataat
     9781 ccggggagtg gagtctggac caaatgattt attttatcga agtgtgtttc tcgatattta
     9841 ccagttgact cggctaatgg tagcagaacc ccaagtggat accagccaac tgtataccta
     9901 tggtgcgtca caaggaggag ctttggcgtt gatttgtgcg gctgttgctc cggaaatcaa
     9961 ggaatctttt gtttgttatc cgttcctaag tgattatcgt gaagcttatc gcttagatgt
    10021 agaaaactcc gcctatgaag agttggctta ttggtttcgc tttaaagacc ccttgcacca
    10081 acgagaagaa gctttctttg aaacattgga ttacattgat ttgcaatact ttgctccttg
    10141 gatcaaaggg aaggtgcatt gggcaattgg ccttgcagat acagtctgtc caccgaaaac
    10201 ccagtttgct gtgtacaatc agctaagtag cgcagctgac aaagaaatgc tattttatcc
    10261 agagtatggc catgaatact tgccaaaatt tggcgatcat atgcggcagt gtttagccga
    10321 cgttttgaga gaaaaggagt agaatgctat gttgacattt ccaaaagatt tctggtgggg
    10381 tgcagccaca tctggtcccc aatcagaagg ccgttttcaa aagaaacatg ccaatatgtt
    10441 tgatcattgg tacgatattg aacctgaagt tttttatgac cgagtggggc cagatatggc
    10501 ttcgaacttt tacaatagct accgagaaga tatcgcattg atgaagcaaa cgggattgaa
    10561 ttcccttcgc acctcgattc aatggacccg tctcatcaag gatttggaaa ccaatgaagt
    10621 ggatccagtg ggtgttgctt tttacaataa tgtgattgat gagttcctct ctcaaggtat
    10681 ccgaccgatt tttaacctcc atcattttga tttgccagtg gagctttacc ataaatatgg
    10741 cggttgggaa tcgaagaaag ttgtcgattt gtttgtggga tttgctgaac aatgttttgc
    10801 tttgtttggt gaccgtgtga aagactgggt gacccacaat gagccgatgg tggtagtaga
    10861 tggggagtac ttgttccaat ttcattatcc aaaattggtg gatggcaaga aagcagtaca
    10921 agtggcatac aatttaaatc tcgcttccgc taaggtcatg gcgaagtttc gcgaaatggg
    10981 tcaaccacaa gctggtggcc ggattggtac cgtgttaaac ttgacgccaa cttacgcagc
    11041 ttcagatgct gaagcagatc aagaggcagc ccgaattgcc gaactgtgga acaataaaat
    11101 gtttctcgac ccagccatca aaggggaatt tcctgtggaa ttgctagaat tgctgactaa
    11161 ggatggggta ttgtggcagt caactgctga tgagttagct ttgattaagg aaaatactgt
    11221 tgattttatc ggagtgaatt tctatcatcc aaaccgggtc aagacacctg acattgctcc
    11281 aaattcagtg ggtgcctgga tgcctgatcg ttattatgat gcatatcaaa tgcctggccg
    11341 tcgcatgaat attgacaaag gttgggaaat ctatcccaaa gcagtgtatg acattgccat
    11401 caatattcgc gataattatg gcaatctacc ttggtttgtt tctgaaaatg gcatgggggt
    11461 ttctcgggaa gaacgcttta tggatgaaaa agggcaaatt ctagacgatt accgcataga
    11521 atttatcaaa gagcatctcc aatggttgca taaagggatt gaagaaggtt ccaactgctt
    11581 tggctaccat ctatggactc ctatcgattg ctggtcctgg agcaatgcct accgtaaccg
    11641 ctacggctta atcgctaatg atattcacac tcagattaag acgattaaga aatctgggga
    11701 atggtttaag acattggcgg agaataatgg tttttaagaa gatttaggag ggaggagtga
    11761 atttgttttc aaaaactcct ttgtgttgga gtaaatggaa aatctgaacg aacaataggg
    11821 tttattgatg aaaagtcatt ctagtttctt cagtgctttg tcaaatggtg ttgttttgac
    11881 caaatcacac tttaggaaat gctttggcat ttcctttttt gatgtaccct gcacgggtag
    11941 caactttgta gctgatggaa tctgaaggaa acctaaagca aaaaattata tcatagttca
    12001 tccattttat aactaaatca aatatcattc gaattcaagg aacagtggat gtgtttattg
    12061 cgataggtgg ttcttttgga ggcgcaataa tagctttgtt aatatttatt taccttaaca
    12121 aaaagaccaa taaacatagc taataaagtt caagtaaaga gatatactgc atccaacgat
    12181 attttttcat aaagttattc ttaactttat gattcttgat ttagatcaat gccaacacca
    12241 actttatttc ctaaaatagg accataaaag tgaaaaggag tgacttcaat atgtcagatt
    12301 acaaaaaaat ttacgaaaca acagcgacta ataccggtgg ccgtgatgga gtgagctatt
    12361 taaatgatgg atcatttgaa gtgaagattt ctacacctaa agaaatggga ggatctggga
    12421 gtggagtaaa cccagaacaa ttatttgctt taggatacag tgcttgtttt cacggggcat
    12481 tagaaattgt taagggtcaa cgtaaaattc atcaatcttc acaagttaca catacagtaa
    12541 aattatacaa aaagccagac gatgtggact ttaaattaag tgctgagatt caagttgcaa
    12601 ttcaagattt agatttagaa gaagttcaaa aaattgctga agaagcgcat aaagtttgtc
    12661 catattctaa agcagtaaag gattccattg aagtttcagt acgcgctgtt ctttatgatg
    12721 cagataaaga aaaataagta tagaaaatcg ttcactgatg atattacatg agtgaacgtt
    12781 tttttatgct atactaacca caaaaagagg agtagtaaga acatgattga tttcacaaaa
    12841 tatgatcatt gtataagtac aatgccttta tttcagggat tgtccctcac tgaaattcaa
    12901 tttatccaaa gtgatattac agaacaagct ttttctgcag gacagtttat ctatcaagca
    12961 gaagatatac ttgaatctct ttatcttatt caagaaggaa aagtaaaagt ttatcgctta
    13021 ggttcttcag gtaaagaaca ggtgattcgt attttagaac agggtgattt tgtgggcgag
    13081 ctggcattat ttgaagaaca acattatgga tgctttatag aagcaatatc accaactaaa
    13141 gtatgttcca ttcaaaagga caactttaag cggattttac tatcgcatcc ccaaatagct
    13201 ctagaacttt tatcagaaat ggcgagacgt cttgataata cagaacgatt aactgcagga
    13261 atcacgactg aaagagcaga aacacgctta attcagtatt tgttagaaga aaatataaag
    13321 caaaatacag agcagataaa attggaaact acgaaaaaag aacttgcttc ttatttaggg
    13381 atgactccag aaacacttag tagatattta acaaaactca ctaggaataa tctaataaag
    13441 cagccaattc ctaatataat cgagttaaca aatgtaactg aattgaaaaa tttaatggat
    13501 aaggtgaggt agataagaag aaagggagaa gcaaaatgca tctccccaaa atgagcttat
    13561 tccttgattt caaattttga aattgctaga tagactgcaa tcatacattc ttgataacaa
    13621 tcttgatcgt attttccgtt tctccacgat tttttgtgta tcaacgagct gaatctttta
    13681 agaatggctt caatggcttc ttcatttcca gcttttgcta aaataaaatc ttctttcagt
    13741 gtattcatag accatcttcc tttccataaa tttaaatatt atttatggga ggtggtcgta
    13801 ttaaaagtga aatcgagtaa gttttaaata agcaattctt ggaatttttt ataaagacgg
    13861 tgctttaggt tagtagtccc ttgtcttgta atacctaaag attctccaat ctctttatct
    13921 gtcttctcaa aaataaattt ttcaacaaga aatatctgtt ctttttcttt taataaagga
    13981 ataatctcag ctaattgagc atcttcaact agaaaagtta catttaaaat ggtaacaggt
    14041 tgcttgattt tgattttatt ttcagtaaag aaatgttgag ggaattgatc gaattgttcg
    14101 tggtcactta tttttttctg ctttttaaaa taggaatttg cagtattctc aatgaccttt
    14161 tcaaaaaaac gaataagttg ggcattcacg cgttcatcat tttctgacat cctactcact
    14221 tcttccaata aaatttaaaa ggagaaggaa cagaaagcgt gaaagaagct aatgcaatct
    14281 atactgccaa taacctctat aagctctttc tacattgatg ctactatcaa tgtttattct
    14341 gggaagaata ttatgagtta agttttcgta ggaaatgctt aatccatgat cattagttaa
    14401 aattttgaag atttctttat ttgatagtgg gcggccggca ccctttaaaa ttgaagcaat
    14461 ggttaaactg atagtcgagt atgcaatcga ttgtttattc ttattggttc gttcactaat
    14521 atatagttga tgttgtgcct ctttagagat ggtcgctgat gatttcctag caaaatcttt
    14581 atttttgttt gaattttcgt gtgattccat actatctaag gtaaaaagtc gagattttaa
    14641 ctcataataa tctttactaa ggttcctcct ttcctcaagt atcattctca tttgtgtata
    14701 aattgcatct ttttcttctt ctagtttcat cttcttctcc tttcataatt aacaacgaat
    14761 aaaaagcatg aatggacaca aaagaaatat ttttttagaa gaaataaaaa gtgaacgttc
    14821 atattgaaat tgttttaaaa c
//
LOCUS       DAJSVD010000066        14703 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSVD010000066 DAJSVD010000000
VERSION     DAJSVD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.067"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(30..743)
                     /locus_tag="ON498_001838"
     CDS             complement(30..743)
                     /locus_tag="ON498_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3940085.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            918..2342
                     /locus_tag="ON498_001839"
     CDS             918..2342
                     /locus_tag="ON498_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3940086.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2641..3348
                     /locus_tag="ON498_001840"
     CDS             2641..3348
                     /locus_tag="ON498_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3940087.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3335..3658
                     /locus_tag="ON498_001841"
     CDS             3335..3658
                     /locus_tag="ON498_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3940088.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3747..4784)
                     /locus_tag="ON498_001842"
     CDS             complement(3747..4784)
                     /locus_tag="ON498_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3940089.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5105..6088)
                     /locus_tag="ON498_001843"
     CDS             complement(5105..6088)
                     /locus_tag="ON498_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR3940090.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6503..7273)
                     /locus_tag="ON498_001844"
     CDS             complement(6503..7273)
                     /locus_tag="ON498_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940091.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7266..8201)
                     /locus_tag="ON498_001845"
     CDS             complement(7266..8201)
                     /locus_tag="ON498_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940092.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8517..10166)
                     /locus_tag="ON498_001846"
     CDS             complement(8517..10166)
                     /locus_tag="ON498_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3940093.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10283..12886)
                     /locus_tag="ON498_001847"
     CDS             complement(10283..12886)
                     /locus_tag="ON498_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3940094.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12898..13947)
                     /locus_tag="ON498_001848"
     CDS             complement(12898..13947)
                     /locus_tag="ON498_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR3940095.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13973..14287)
                     /locus_tag="ON498_001849"
     CDS             complement(13973..14287)
                     /locus_tag="ON498_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR3940096.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14303..>14703)
                     /locus_tag="ON498_001850"
     CDS             complement(14303..>14703)
                     /locus_tag="ON498_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3940097.1"
                     /translation="ASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGK
                     NIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILA
                     ELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4353 a         3053 c         2485 g         4812 t
ORIGIN      
        1 taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca
       61 aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca
      121 acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca
      181 aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt
      241 tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg
      301 actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt
      361 cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc
      421 gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg
      481 ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact
      541 tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct
      601 ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt
      661 ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc
      721 atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata
      781 ctttaacata aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata
      841 tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa
      901 caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt
      961 ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga
     1021 ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct
     1081 ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg
     1141 ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg
     1201 gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata
     1261 acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata
     1321 aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt
     1381 ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta
     1441 ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag
     1501 cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac
     1561 tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc
     1621 aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt
     1681 tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata
     1741 atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc
     1801 aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg
     1861 atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac
     1921 gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg
     1981 atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg
     2041 cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt
     2101 atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg
     2161 ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat
     2221 atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat
     2281 tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat
     2341 aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc
     2401 aataacaaat actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca
     2461 aatgtagtga tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact
     2521 acgtccagtc ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag
     2581 aatattcaca agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
     2641 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt
     2701 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg
     2761 gtcgtcacgt tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc
     2821 atgcttgcta gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc
     2881 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
     2941 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
     3001 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
     3061 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
     3121 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
     3181 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
     3241 attctggtat atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca
     3301 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
     3361 gactatttta ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact
     3421 gaaaaaattc gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
     3481 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
     3541 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
     3601 cttattggtg atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat
     3661 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
     3721 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
     3781 cctaatatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
     3841 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
     3901 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
     3961 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
     4021 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca
     4081 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
     4141 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
     4201 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
     4261 agttgattca aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg
     4321 cggttggcat tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc
     4381 aatgaggtca acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg
     4441 acaatacgtt cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct
     4501 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact
     4561 ttcccgactc catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga
     4621 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
     4681 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac
     4741 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
     4801 cttcatcaag ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt
     4861 tccactttta ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg
     4921 ttaaaaaaga ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa
     4981 ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg
     5041 ttcttattac ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa
     5101 gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc
     5161 gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa
     5221 atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt
     5281 ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg
     5341 tatgttccct cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca
     5401 tgatgccatc tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat
     5461 ctccgttgat cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag
     5521 ggatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag
     5581 aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt
     5641 ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa
     5701 tcaattgtgc cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta
     5761 cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag
     5821 caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct
     5881 gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga
     5941 aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga
     6001 catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg
     6061 gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg
     6121 aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag
     6181 tgaacagctt aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca
     6241 caattccttt tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac
     6301 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca
     6361 gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
     6421 acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa
     6481 cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc
     6541 aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg
     6601 gcacatcgat tgttccaagg aatccgtaac ggaaaccgaa gatcatataa acgatcggat
     6661 tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac
     6721 ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact
     6781 gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg
     6841 taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg
     6901 ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga
     6961 tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag
     7021 agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac
     7081 caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca
     7141 tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga
     7201 cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat
     7261 aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt
     7321 tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc
     7381 tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca
     7441 tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca
     7501 aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc
     7561 agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa
     7621 tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc
     7681 tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt
     7741 tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt
     7801 gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc
     7861 atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc
     7921 gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg
     7981 actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact
     8041 aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca
     8101 tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca
     8161 taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat
     8221 agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc
     8281 gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag
     8341 tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa
     8401 caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     8461 acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat
     8521 cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct
     8581 actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa
     8641 ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca
     8701 aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg
     8761 acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc
     8821 cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg
     8881 gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg
     8941 ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta
     9001 aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt
     9061 ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag
     9121 ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca
     9181 ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt
     9241 gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc
     9301 ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg
     9361 acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat
     9421 ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc
     9481 agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc
     9541 tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg
     9601 attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga
     9661 agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct
     9721 agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt
     9781 ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct
     9841 tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag
     9901 gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg
     9961 atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct
    10021 tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt
    10081 aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt
    10141 aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt
    10201 atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt
    10261 ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa taatcatagg
    10321 gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa
    10381 ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc
    10441 cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa
    10501 tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta
    10561 acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt
    10621 tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa
    10681 aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt
    10741 catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt
    10801 cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca tactctttta
    10861 aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga
    10921 atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat
    10981 caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta
    11041 aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc
    11101 cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt tcttccattt
    11161 cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa
    11221 ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt
    11281 ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca
    11341 aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa
    11401 aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta
    11461 atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat
    11521 ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa
    11581 tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg
    11641 gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac
    11701 tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat
    11761 cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga
    11821 ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag
    11881 cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac
    11941 catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt
    12001 taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt
    12061 aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt
    12121 ctacataaga aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct
    12181 ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa
    12241 tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg
    12301 caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg
    12361 gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg
    12421 gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat
    12481 gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca
    12541 ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg
    12601 attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc
    12661 ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat
    12721 cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat
    12781 ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc
    12841 attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta
    12901 ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc
    12961 attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat
    13021 attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa
    13081 aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa
    13141 aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat
    13201 agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc
    13261 taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa
    13321 gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc
    13381 catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga
    13441 catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac
    13501 ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt
    13561 tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca
    13621 agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc
    13681 agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga
    13741 aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa
    13801 tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa
    13861 ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat
    13921 ttctcttcct tgtttcttga attccattag taactccccc acgatatatt ttttatttgt
    13981 taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc
    14041 ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg
    14101 caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca
    14161 tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat
    14221 tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt
    14281 ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc
    14341 tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta
    14401 aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat
    14461 caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca
    14521 ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc
    14581 ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca
    14641 caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg
    14701 cat
//
LOCUS       DAJSVD010000067        14550 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSVD010000067 DAJSVD010000000
VERSION     DAJSVD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.068"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            4..816
                     /locus_tag="ON498_001851"
     CDS             4..816
                     /locus_tag="ON498_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR3940098.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ON498_001852"
     CDS             813..1613
                     /locus_tag="ON498_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3940099.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ON498_001853"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ON498_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR3940100.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ON498_001854"
     CDS             3110..4819
                     /locus_tag="ON498_001854"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3940101.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ON498_001855"
     CDS             5031..9389
                     /locus_tag="ON498_001855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR3940102.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ON498_001856"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ON498_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3940103.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ON498_001857"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ON498_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3940104.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ON498_001858"
     CDS             11227..11520
                     /locus_tag="ON498_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR3940105.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ON498_001859"
     CDS             11517..11828
                     /locus_tag="ON498_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR3940106.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ON498_001860"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ON498_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3940107.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ON498_001861"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ON498_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3940108.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2584 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc
//
LOCUS       DAJSVD010000068        14486 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSVD010000068 DAJSVD010000000
VERSION     DAJSVD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.069"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(324..893)
                     /locus_tag="ON498_001862"
     CDS             complement(324..893)
                     /locus_tag="ON498_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3940109.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ON498_001863"
     CDS             complement(967..2256)
                     /locus_tag="ON498_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940110.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="ON498_001864"
     CDS             2610..3458
                     /locus_tag="ON498_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR3940111.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="ON498_001865"
     CDS             3472..4545
                     /locus_tag="ON498_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR3940112.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ON498_001866"
     CDS             4535..6706
                     /locus_tag="ON498_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HCR3940113.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="ON498_001867"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="ON498_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR3940114.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="ON498_001868"
     CDS             complement(9117..11303)
                     /locus_tag="ON498_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR3940115.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="ON498_001869"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="ON498_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR3940116.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="ON498_001870"
     CDS             complement(11525..11965)
                     /locus_tag="ON498_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR3940117.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="ON498_001871"
     CDS             complement(12039..12512)
                     /locus_tag="ON498_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HCR3940118.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12822..14288)
                     /locus_tag="ON498_001872"
     CDS             complement(12822..14288)
                     /locus_tag="ON498_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3940119.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4128 a         3025 c         2555 g         4778 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 caagggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaataaatg gtgttcaatc
    12661 agctgacctt cagcattagc tcaaaaaacg gcaaaatatg aggagctatt tttgtttggc
    12721 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaga
    12781 agtagcttct gtcacaacct cttgtaacaa gtatttctat tttaaggctg aaccactgtt
    12841 tctggttcag taatttcttt gtttttgtct cgacgagcaa ttgctggcaa gatcatacct
    12901 aataagatca agacaattgg tgtgatgata ttcgtgaata attggaacca ccatgcttta
    12961 ggatcagcag tataatctac ttttggcacc attcctagga tacatgcaaa cgcagtgaag
    13021 gcaaagcacc aagcaccaaa gataaaaccg atttttggat ttttaacaaa tttatattca
    13081 gaattgaatt tcttataagc tttattcaac atcatatatg ccaagaacac ccataggtaa
    13141 cgcattggca tgacaactga gttcaagttc gtcaaccatt ttactaattc attcatatct
    13201 ttgataccaa agatcggcaa caaaataatg attcctacta gtatacctgt cagaagataa
    13261 ccattcacca atgtaccttt acgattacgt ttacgtagcc agtttggtac aaattctgga
    13321 tccgcatctg ctaacaagat ttttagtggt gcatcaattg aaaaggctaa tgcggagatt
    13381 tgtccaatca tgtttgtcaa tgcgtaaatg atcatcaatg agttaccgat accatagtaa
    13441 ttacctaaaa tttgaaaagc actgtatgcc ccatttgcca tcaagtcttt agggatatta
    13501 tcgctagcaa acagcatacc cattgcgact gagcctaata cagcacaaat ccctaccatt
    13561 gctgctaaga agatcatccc tttaggaaat tcctttgcag gatttcttgt agagttgaca
    13621 taaggggata ttttttccgc ccctccaact gcaaatacta gcatcgaaac ggttgtgaaa
    13681 taactgaaat caaatgttgg aatatatgtt ttcacttgat tcatatgtgg tgtggcaaat
    13741 tccatcgttg gattgatgaa cggtgccgtt acagctaaaa gaataaataa gatcgacatg
    13801 acgaacatcg ctgttccggc cagtccgcca atcactttca aagtagaaag tccttttgtt
    13861 gataaatata aaaaggcaag aaatataata agtgaaataa ctgctaccat ttgtactgac
    13921 atcgtattta cgatatttcc atttccttgg actgcccaac caccagcaat cagaattgct
    13981 tgaggctttt gtgccaaata aggaatatgg acgacccagt aagtccaagc ggcatagtat
    14041 gccagtcgtt tattagatgt cgtattttcg acccatgaac tgacgcctcc tttactatcc
    14101 ttgaaggctg atcctaattg tccaacgatc aatgcgtatg gaataaaata aatggccaaa
    14161 atcaatagcc aagaggtgac aactgatact ccttgctgag catagttatt aactacattc
    14221 cccatgcccc aaaccatgtt aaatgcaatt aacgcaacag taaaccagcg taattgtttg
    14281 ttgttcattc gttctctcct ttatgataaa aatttgttat ccagatgtaa tcatattcaa
    14341 tttttaacaa acttacaaga cagtgttatt ttatgtgtgt attcttttat ttaatacaca
    14401 tcgttttttc atattttttc catcaaaaaa ataagttatt ttcttttatt tcattgacct
    14461 tattgaattt caagctattt tgaatt
//
LOCUS       DAJSVD010000069        14191 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSVD010000069 DAJSVD010000000
VERSION     DAJSVD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.070"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ON498_001873"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ON498_001873"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3940120.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ON498_001874"
     CDS             complement(2260..2511)
                     /locus_tag="ON498_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR3940121.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ON498_001875"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ON498_001875"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR3940122.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ON498_001876"
     CDS             3461..3679
                     /locus_tag="ON498_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940123.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ON498_001877"
     CDS             complement(3968..4480)
                     /locus_tag="ON498_001877"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3940124.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ON498_001878"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ON498_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3940125.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ON498_001879"
     CDS             complement(6933..7376)
                     /locus_tag="ON498_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR3940126.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ON498_001880"
     CDS             complement(7377..8165)
                     /locus_tag="ON498_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3940127.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="ON498_001881"
     CDS             complement(8162..9103)
                     /locus_tag="ON498_001881"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR3940128.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ON498_001882"
     CDS             complement(9280..9867)
                     /locus_tag="ON498_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR3940129.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="ON498_001883"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="ON498_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR3940130.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ON498_001884"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ON498_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3940131.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ON498_001885"
     CDS             complement(12784..13719)
                     /locus_tag="ON498_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3940132.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..>14191
                     /locus_tag="ON498_001886"
     CDS             13905..>14191
                     /locus_tag="ON498_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940133.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGR"
BASE COUNT         3959 a         3159 c         2506 g         4567 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc g
//
LOCUS       DAJSVD010000070        13846 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSVD010000070 DAJSVD010000000
VERSION     DAJSVD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.071"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            375..1814
                     /locus_tag="ON498_001887"
     CDS             375..1814
                     /locus_tag="ON498_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3940134.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2109..2327
                     /locus_tag="ON498_001888"
                     /pseudo
     CDS             2109..2327
                     /locus_tag="ON498_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2576..2791
                     /locus_tag="ON498_001889"
     CDS             2576..2791
                     /locus_tag="ON498_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940135.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3008..3264)
                     /locus_tag="ON498_001890"
                     /pseudo
     CDS             complement(3008..3264)
                     /locus_tag="ON498_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3451..4362)
                     /locus_tag="ON498_001891"
     CDS             complement(3451..4362)
                     /locus_tag="ON498_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3940136.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4381..5385)
                     /locus_tag="ON498_001892"
     CDS             complement(4381..5385)
                     /locus_tag="ON498_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3940137.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5382..7505)
                     /locus_tag="ON498_001893"
     CDS             complement(5382..7505)
                     /locus_tag="ON498_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3940138.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7510..9957)
                     /locus_tag="ON498_001894"
     CDS             complement(7510..9957)
                     /locus_tag="ON498_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3940139.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9944..10333)
                     /locus_tag="ON498_001895"
     CDS             complement(9944..10333)
                     /locus_tag="ON498_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3940140.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10393..10896)
                     /locus_tag="ON498_001896"
     CDS             complement(10393..10896)
                     /locus_tag="ON498_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3940141.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10909..11133)
                     /locus_tag="ON498_001897"
     CDS             complement(10909..11133)
                     /locus_tag="ON498_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940142.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11237..13147)
                     /gene="ltrA"
                     /locus_tag="ON498_001898"
     CDS             complement(11237..13147)
                     /gene="ltrA"
                     /locus_tag="ON498_001898"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3940143.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         3924 a         2891 c         2426 g         4605 t
ORIGIN      
        1 tcctatggaa ttgaatatca tagcaatgta tttgaaaagc aaattggaaa acaaataggt
       61 gcaacaccat aaatatgaaa aaattgggaa aaagtagaat ggaaatttgc ttacgtctca
      121 cgagatagct cacactatta atctgaagaa caatgaagtc gacttttaga aattgctaga
      181 agaaagtaaa gcgaattatc actagttact atagagtaag ttcaaataaa gcatctccaa
      241 cttacatggt tacatgagga tatcaaatcg aaattactca agcttcctat ggctagagtc
      301 gcgagaagtt gttgggcagt agacataggt tatattgttc ttagctacac gatcattaga
      361 aaggaaatcc tattatgtat agttatcaag aagatgcaaa agtccaaaaa aatcgctggt
      421 ggattttgat tgctatatct atgtttacct tcatgtccac attggatggc agtattgtca
      481 atattgcact acctaccatc tcagatgata tgggcatacc aatgaatcaa gcggaatggg
      541 tagtgtccgt ttatctgatg attgtctgtt cttgtttgtt gctgtttggt aaaattggcg
      601 atagccgtgg gaaaatcaaa gtatttcgga ttggtacgct gatctttgtc ttgggatcgc
      661 tgttgtgtgg gttcaatcat tcgctaggta ttttattgtt cgcacgggtg gtacaagcca
      721 ttggtgctag tatgactatg gctacaaatg ccggaatcat cacggaagtc tttcccatac
      781 atgaacgtgg tcgggcattg ggagcaatag gtgcgtttgt ttcattaggc tcgattgctg
      841 gaccaggtct tggcggcttg attttgtccc aattttcttg ggcgtacatt ttttggatca
      901 atgtaccggt gggaatcgta acgatgttga ttgctgaaaa attcttacca aaagatatca
      961 ccatgagcgg ccatacaatc gatggagggg gctttgctgc cattgcggct acgattatca
     1021 gcttttttgg agcaatcttt ttggggcaag aaagtggctt tttccatcca gtcccccttg
     1081 tactgttcgt aatttcagtg attgcctttg tcagcttttt aaaagttgaa aagaaagcag
     1141 cctctccatt gatttctttc ggtattttta agaacaagat ttttaccatg agcttgatta
     1201 cagccttgtt gattttttct tccaacttct ttgtcaatgt agtgattcct ttttatctgc
     1261 aaaatgctcg cgggttgagt cctagctatg ctgggctgtt gatgatggtc tttcctttga
     1321 tgatggtcgt cggggcgcct ttaagcggct acctgaccga ccgggttggc ccggagattt
     1381 tggtattagt aggcttgggc ttattgtcgg taacccaggt catgtatatg tttttgtcag
     1441 tggactctcc tttgtggcta tacattgtcg ctactgccat catgggtgcc ggcaatgcct
     1501 tgttccagtc ccccaataat acgatggtta tgagctcggt cactatcgaa aacttgggac
     1561 tggcaggctc gatgaattcc tttgcccgaa atttggggat ggtcagcggg atttcgcttt
     1621 ctacgacggt cttatatcaa gcgatgtctg ctaagatggg ccagcaagta actaactata
     1681 tcgcaaatcg gccggatgtc tttttatatg gaatgaagat taccttttta ggcagctttg
     1741 ttttgtgttt tgttgccttt ttcctgacta tctggcgtat caaaaaacaa ggaggtattc
     1801 agaaaaatgt ctgaaaaaac cactgttgaa gaatttttgg aggcctgcgt atgggtgagt
     1861 gggatttcca tggatgatgg aagatgaagt gaacttctaa aagccgaaaa actcaagtat
     1921 tatcttcaaa atactagtgc tcaatgagat gcttgatttg atcacaagaa gactgcgaaa
     1981 gccgctggca accgcaagct aggtatccca ccgatggact acgttttaga ggctactggt
     2041 tatgaatacg gagccaacgc tctcatctgc ataagaatac atcacccaga ttatgtaaag
     2101 attttcgata cttctgtcaa aaaatcatta agatagataa atgatttccg tctagtcaaa
     2161 ttggttcaga atgtagacac acagatggta agaagtcact cacaattcga gaatggactt
     2221 gtcctatttg ccgtatacat cataatcgaa acgtcaacga tagtatcaat attttgacca
     2281 agggctatga atacaagcat tgccttagaa aacatcaaga accttaggaa caacggggat
     2341 agcttggtta ataagagata ccgctgttta ggcttgcaag tatactcttt tatcaagaat
     2401 tccgtggctt tagtcatgag aggcttaata ctatgaaaat tttctttagc atttttgatt
     2461 taggttcctt ttctaattat aataagatga ccactacagt aacctactga aatatataaa
     2521 cagccactgt agtaccattt tcaaagtagt ctatgagagg agaatcttca ttcttatgaa
     2581 cgctggaatt gcggatatga atttaataaa aaaaaccctt aatgatttta cttccaattc
     2641 aatatcaaaa ggtactggta tcaatttaag cacaataaaa aagctgaaaa gtggtgaaag
     2701 atcagtggaa aagctgaatc ttttagatgc tataaaaata acggaatttg ctatgaaaaa
     2761 tggaaaagct gagattgaaa tatggagata attatcgtct agtgaattac ttgccactta
     2821 gctgaattta acgatccttg gtgcttgaag ggggagattc ttgggaatag agcatacttg
     2881 caagcctatg tctttactaa caacgatatc tcttattaac caagctatcc ccgtagttct
     2941 aaggctttta cttttatcta agcaataatc gtattcaaag gaaatttttg acccttagaa
     3001 agtctcattt tatcagacgt gagcttaggc attgctgaat tttgttaatt cggttttagg
     3061 ttgtgctttg agcataatat gaaagtattc tttatcacga tttcattcat ctaaggtaat
     3121 gtgatgtgat tctgaaattc tttcaaaggc agtttttgca taagctgaca ttacatcatc
     3181 aatgacatgt ttacaatgtt tcactactaa aataaggggg taaccaagaa gaaaaactga
     3241 atgattattt gtatctaatt ccatggtata tcaaccattt cctatataga ctgattatga
     3301 catggattaa aataaaaagg cactatgtct taattgtcgg ttttcgagtt accgaatggc
     3361 attcatcacc cacctataga ggatgggcga cttctgcctg aaataagtta cactacttat
     3421 aacttctaat aaaattagat tgagtcatta ttatttaaca tcaacgattt tccagtttcc
     3481 atttttttgt aatactaatt tgtactgaaa gtagctagtg gtttttgata cattatcaag
     3541 gtattttatc tttgtactca ctagaaaatt ttttccgttt tgtagataaa ctggattctc
     3601 taactccgaa aactttaagt caatattgat tggtcgcatc gcaccatttt caacataata
     3661 ctccaattct ttttcagaag ctgatggata aagtttaaaa aatgtttcta aaaaatcggt
     3721 gatttcttcc gttgttttgg catcaatttc tgaatcgttt tctacttgtt ttgcttcata
     3781 ttttgccttg tctggctttc caatcattgt tggcaaacta gttactaaat gattcctgtt
     3841 ttcatcttca aagattgtca ctcgatatgc agaagaaatt gattgcgtct tttttccctc
     3901 tgtaatatgt tgctccacta aaaatgaaac gacaaattct tccgagtgtt tttcaacatc
     3961 tagaattttt acagattgaa cttcggaact cgtgggaata tctgctcgaa ccatatcttg
     4021 gattaaagcg agaccgtttt ctgtaaggta ctgttcgaga ttactcattc tcttttcaat
     4081 tgcttctttg ttatttttcc atgaaaaata ttctttcgca aaatcctctg taaaactttc
     4141 aacgccagaa gtattcacta ccttctcttt tataaccact ttttcatgag ttgtatgttg
     4201 atcaatggcg gtgaaatttt tataaacgcc aaaagcaaga ctacaagaca ataaaagcca
     4261 aagggcaagc accatttttc gatgcttacc cacggataca actcgttctt ttttcttttt
     4321 tgggctcttt tccttttgat tccgttcaat ttttatcttc attctttttc ctcttttctc
     4381 ttatttgtat cggccagctc cgataagatg ctgccgccaa tagttttccg ttagattcgt
     4441 ccaccccact ggatctcctg catgaaacat gcgattctcc cctgcgtaaa tgcccacatg
     4501 ggtcacataa gtaccggcat cataggttga atgaaagaaa actaaatcac ctggtttggc
     4561 atcttttaga tcaatatgag tcatggcatc atattgatcc tgagccaccc gaggaagttt
     4621 caggtcaact ttggcataac accattgggt caagccacta caatcaaacc ccgtttctgg
     4681 acttgagcca ccaaacacat aggccgttcc ttcatactta aatgcctcat caaaaatttc
     4741 ttggactatt ttgtcatcaa actgagttgt agttaggtat tgcttcacta ataaacagta
     4801 aaacatattg ccataattat accgccaccc accattttcc ttaatcgcga ttgggttggg
     4861 gtaatcgact ttttgaccac ctgatttttc ctttgaaaaa gattcagcta aagcaaaact
     4921 atgcttcttc ccgtttttcc cgacatgatc tagatacccg ccaccatagt tataggcctg
     4981 aataatacta tcttgatcac accctagttc tttggaacgc ttaaccaaag acgcaaaata
     5041 gagacagcct tgtttgatgg atgcctcggt acttaatgaa ttaggtggta atccggctga
     5101 ctcagaactt tgcataacgt cttccattgt gccaccagat tccacttgaa taatcgccaa
     5161 aagaattgga atatactctg ttatatccga ttctttggca tacttttcca ccattgcacg
     5221 gtgtttcaat acttcttctg agacactagt tccgccagga gaagtgccag aactaccatt
     5281 tccctcactg tcttcaccaa acaacaaacc cacacacatc aaaataccca taaaagagat
     5341 ggtcagaaaa gttaatgaaa taacacccac tttcttcaag ttcatttttt ctcacctgac
     5401 cttgattttg gacgatggtt cattcttttt tgtacttttt gattggtctg ctttggcaga
     5461 gtggacctct gtttcacttg ttggaaagaa tttttcttac ttgctgattg gcttttccct
     5521 tttgaaatga tttcttgact agttagtttt cgtttgattt cgggattcag atttgctctc
     5581 acaatccgct ttggtgcttc ttttttcagc atcatatctt tctgaggttg agctgcttta
     5641 tctattggaa tattcgccct tctttttccc tgacgatttg ttgcttcact cagtatcttg
     5701 cgtttttcgt ccattttcgc atttcttcgt tgtcgttgtt tttctcgcaa atccactcga
     5761 tccgcttttt cttggaacag tccgcgcttg aattcttctg gtgctttttt cgacttttcg
     5821 attcctttat ggagattgta tttcaaattc gttggcgtat tccttacctg atcctttact
     5881 tgtttagtct tatctttcac caaagcttgg gtatccaaaa ctttgccgat ttttctccca
     5941 gccaaattca ttcgggattg tttcttatta tttgaaactg gattctcctt agttaagtca
     6001 gatagtattt cgtgatcgtt cggtaatcgt gaattctttc gtagagaaga accggatggc
     6061 ttaaatttgg attttgattg ttcctttgcc aacttatttc cgactgctgc accaccacca
     6121 aaagccaaag ctcgtcccac atttcgattg agttgccgca attttcgatt cattaacatt
     6181 tgcggttttc gcatgacgcg tcttcccatt gactggctat cattactctg gaggttgaac
     6241 atactcataa tgtcgcccaa tttgaaatag ataccggcaa aggtcacaat ttgcagaaaa
     6301 gcaaccatga aaaatggata ggttgccgaa atattgaaaa acatcgtgga gatgctaaat
     6361 gcagtcgtaa tcaccaaagt aacacccgct cgcatcataa tcgtattgaa taaacgaata
     6421 atcgccttct ttcctaaact ttcataagtc ggcaacatgg acaataagaa actaattggc
     6481 agaaacatcg caaagataat gaagagaatt tgcgagaaca acataatccc tgttagaaga
     6541 aaaacaaaga aggaaatgcc aatattgaac aataccaaaa agaccacggt ccctaaacgg
     6601 ttcatggtct tggtaatact gagattcgca ttgtcattgt cttcaatctc tgccttgacc
     6661 gcttcttccc gatctttgcc tttgttttca tctggactca cagatagaat cttgttgacc
     6721 cggtcttcac caatttcttc tatgttggaa tcatcaaatt gcagtagtaa ccaaggttgt
     6781 tggacttgaa tcgaaaacaa actatcccga atcaaatcca cactatcttt tccttggctt
     6841 tctgaattgg gtacaacgat ctcggttcca agggtaaggg ccgcttcact aacatctgag
     6901 ctaaagtcat tgatttttgt gatataggta ggcgcataag caatgaacga acccgatagt
     6961 agaaaaatca tcacaaaatt aatcacggct cgaactgctt tcgtggtttc tcgttttaat
     7021 aatccggtat acgccacata cacccccaga gctaaaatca tcaataatag aaacccaaaa
     7081 taaaatccgg aggtctggag tccattttca gtaataccag ccaatgtttg gatattcttt
     7141 ccaatacttt cagccgtatc cgaaatgaaa tctaatttgt aagcctcttg aaccacataa
     7201 cccgtggcgt tcgacaaata caaactcacc gtccaaatga aattagtaat ggcataaaga
     7261 ccatacatca cgcttttccc taagccatca ccccaattcc aaggcaacca atcccaagag
     7321 ctatccacaa agaaatctag ttgatagttg ttcagtgaat atttggaata tagatttcct
     7381 gcttcaactg tatcatcaac tagccctgtc gcctcggcta ccgtaccaac taaactcaat
     7441 agaactatca gagtgagtat gctaataaaa acgattccca caatccgaaa aagtttcttt
     7501 ttcatcccct cactccattc tctcttgaac aggtggacgg gtatcaaagg catggaagag
     7561 ttcttcaaag actggatgta cctgaaccac gcctacccga ccatacaaat cctgcatcaa
     7621 gcattggcca ttttccaaat ctcgtaggcg tttttgattg ctttcatctt ctcgatccaa
     7681 gccaaagaat tccaaggttt tcttgatttc atgaatatcg gtactgcgaa acgcaaactt
     7741 caacccgata ttattcttca agcgttcatc cagtaaatca tcactatttt gcgtgacaaa
     7801 gtaaaccccg gcattcatag cccgcccagc acgaacgagc ttattcgata atgttttccc
     7861 ttgcgctact tgaagaaaac tccacgcctc atctaagtct acaattttga agatttctcg
     7921 attactatgg ataaagtcca aggcaaaagt ggaaatgacg attagcatgg aaacactaag
     7981 caattccatg gtggtatatt cttcaaaagt ggtttctgca tctggtaaaa ccaaatccgc
     8041 tacttgaatg atatttaact gcctatctaa actaatcgac tgggtaatcg aaccatctga
     8101 gaaaagcaaa tgggcaaaat cataatcggt aaaggactcg atgtgatccg cgatattttc
     8161 cgcaactggt gagccttctt ttcgaagctc attaattact tgtaataatc cccgttgatt
     8221 gctttgggta accgtccgaa tggccttacg caacactgga aacttctccc catctcgact
     8281 agaaattcct gttaaaaagg tcagaatatc aattgctaga ctttcagaat ccttcttttt
     8341 ctccataatc acataaggat caagtaatcc ttgatttttt ggcttactag ttaagttgag
     8401 aatattgatt tcatccgcaa tttctggcaa ggtttctttc cagccacccc gttccccttt
     8461 tggatcgaca atcacagctt gtccaccaaa aaggacggcg taataaacta agagattgtt
     8521 actaaaggac tttccgccac cgagtgaacc gaggaatgcc gctgctagcg cattggtaac
     8581 tgaatccttg accccttgtg aggccaatgc cggtttcagg taaacattgc gccccgtatc
     8641 tacgttataa ccaaagtaaa tgccttccaa ctcccctaac atctgagtgg cgccaaaacc
     8701 aagtccggcg agaaaatcag acgtaacata ctggatataa tcattcatgt atcgcttact
     8761 agaaggtaag aattcttcat gtagccccat catatcgcca aaaggccgga ctagttttac
     8821 attggtatca tcataaaaat ctagcacttc atcacaccgg cgctttagtt catctactga
     8881 ttcggcactt actcgaacca catagctcag tttgtacatg gattctttgg actgatccaa
     8941 ggtagtttcc aattcatcta cggaatccaa tgcatctaag acattggaat ttgtttcatt
     9001 gtcagattga taggcatggt tgtccaagtc ttttaattcc tttttcttat tgcgaacggt
     9061 agcaagtgcc tttttgttcg tcacaatctc cacattcata ctagtgtcaa ttgggaatgt
     9121 aaattgttgt tgctggtaat agaaaagttc tgaggaggga aactccattt cccccacaat
     9181 tgtattaatt gttaagtacg ctacatatga ttcatgattc tcatgttcca tgcataagta
     9241 ccggggcttt tcttcaatca aacaacgact gggacgtaat aagtcgtaac gtttcaccaa
     9301 agtttccgac ttcagctttt tctttggtag ctgaaattca tattcttcaa atggtgtacc
     9361 cttttcgcca tagatatgtt caatcaaata catcagatca ctcggtgtga ctcgtctgac
     9421 tttaaatctt cgagccaatt tactttccat tagtttttca agcttggtat atcggcgaat
     9481 ttcttcattg gataaggaaa caaaatcgcc catcaaatga tgattcactc catagacaaa
     9541 ttcttgaaag ccggagaaga aagatttctt gagattcttt agattgacct cttcatctgt
     9601 cgcaatcagt ttgaagccaa taaaaaagcg ataatcaatc tgactatcgc caatcatgga
     9661 tactaatgcc tctgtctgta aatcaattcg ttgtttcgct acttctgtta atcgcccggt
     9721 aatttccttt tttgatcgtt cttgcgtggc acgaatacta ctttctgtgg caatttgtaa
     9781 ggcatgaatc ttgccttcac gattttgcgc aatcaattga cgaaagttat cgtggacttg
     9841 atatttctgt tcaggtgaca aaaaggagta attgtaaggt attaactcat agtaggcaaa
     9901 acattctccc tcttgattaa agacaagatt attttcaata tacttaatcg ggtacatagt
     9961 tcacactcct taccagagta atgggttcgt cccatcgttt cttctgttct ttgactttct
    10021 ttcctgcgaa agtgacctta aatcgtaagt gatagctaat aaaagatcgt aaaaatccca
    10081 atggtttctt accgtcaaac gttttttgcg acatgaacca agtaactgcc accggtatac
    10141 ctaagtattt taaaaaggcc ccctctatca tagatagtgg aggcacatgg gcaaatacga
    10201 tgacaaacag caaggataaa acaaaccaag ccatctggtt aaaagtcaca ggaaaaggca
    10261 gttgaaagtc attaattgca taaatgacct tttcaactgc ccaaatactg gtgtagcttt
    10321 ttattttctt catcaaatca ttcctttaaa aagaaggata gtttagaaat caactaccct
    10381 tcaataattt tgttaatatg gatactcaaa aacacctcga ctcgttacca aataattgcc
    10441 tccaatctcc aagtctctac cacacgcatc aaaatcaata tacgtcttca agtgttctgg
    10501 tacttctcca aaatcaccag cttctacaat caagaaatat gctacatcac tcatatcatc
    10561 gcaatctgga taatagataa tatcatccac atgctctacc atctcctcaa aagaattgaa
    10621 gaaagcatgt tgaatctcag aagcaacttc accaactggc gttccttcaa gttcctccgc
    10681 taagccacac aaccgattaa tttcctcaat tggcgtatac tcatcaatct caaagggaag
    10741 ttcataatca tgaattgcat attcctcgta ttcgtcattt aacccaattc gttctttcac
    10801 ttcatcaaaa tcgacaggtg gggtaaacca cgcccccacc aattcacctt cgttatattt
    10861 cccgaggttc gcaatataaa cacgcatctg ttccatcgtg cttccccctt acgcaccgac
    10921 aattcggtta aacaaactga gtaagacatc cttcacccca gaagcattaa agaccaatcc
    10981 gaccgcaatc aaagcaatca ccaaaaagcc aatcagtttg gaaaactccc gcttaaatcc
    11041 taaaaacaaa ccaatgacca caattgccag taagactaag gattgggcat tggttaaaaa
    11101 ccattgatat atggataggt aagttcctga tattataaat atctcaaact tttcccccca
    11161 ttcacacgga gcgtgcgact ttcaccgcac tccgcgttcc atcgtgttta tattgaaaag
    11221 tatttatgta gtagacctat atcaagttta attcttgatt gccagctaat actctatatt
    11281 tatttagtct tatgagctgg actttattta gcttcaatag agctatatat ttttgtattg
    11341 tttcttctgt tttagcgtgt attagacggt gtaccgcctc ttttaccaaa ataagatttt
    11401 tataatcgtc atttccacca gctccagttg gtacaatatg atgacaatgt atttcattag
    11461 cttgttgaaa ttcttccaga gttatagcac attttccatg ttgagcagaa aatagcgaaa
    11521 ttcgattgtc tgcgtattct attgtgcgat tttccaaaga taatttcatt aactggtaca
    11581 tcagtaattt atcaacactt agattggtgt ggataaattt tcttccctct agagtgtagc
    11641 aattcgctaa atacaccttt gccattggtt ttctgtgttg gatatatcca acagggaaaa
    11701 taggttcgcc ctcgccagca acataccgaa gcattttaga tttaccatat cttgttgcct
    11761 cctttgtcgt aagttgtctg cctgtttttc tcaatctgcc ccttttgttt gtacctaatc
    11821 tgttagtgaa gatagccatt acagctctat tcagtttcat acaatccaga tttacattcg
    11881 ttgcaatctg ataataattt tgcataccta ataccattga attataaagt cttgtttctt
    11941 ctaattccgt tttccctttt gagggagaag caattttctt tgcctgtgat accagcttgt
    12001 ctttttggtt tttgaattgt ttatcactta tatgtgattt aacaactaac ttctttcttc
    12061 tcggtatcat tttaatcttg aaaccaagaa agtcggagta tctttttcta acatttacaa
    12121 ttcttgtttt ttcttgtgat acttccaatt ttaatcgttg ttcaatccat tgagtgattg
    12181 ctattttggc tttttcagca ctttccttgt agcggcagaa aactctaaaa tcatctgcat
    12241 aacgaaccat atacatttcc tttaactttg ttttcttcat ttctttgtaa gcgttgcttt
    12301 tacatgggct accgctttta ttgatatgaa ccacatagtt ttttgtaaca ggattttcct
    12361 gccattggct ttctacccaa tggtctaact cattaagaac aatgtttgct agtagtgggg
    12421 atattattcc cccttgcgga gttcctttct ctggatatgt tattgtaccg ttagtcattt
    12481 tgattggagc ttttaatatc ctttttaaaa tgaatatcaa gtgcttgtcc cgtattccca
    12541 tagcccatat ctgttttatc agcttggaat ggttcacgtt gtcaaagaaa cccttaatat
    12601 caaattcaat cacataatgt agctggctga tttgtaggcg gttataggtt gcttttattg
    12661 cattttcaac tgacctatta ggtctaaaac catagctgtt ttcactaaat ttagcttcgc
    12721 agattggttc taatacttgt tttatacatt gttgtataag cctgtcccac atacaaggaa
    12781 tacctagagg tctggtttta ccatttggtt ttggtatttc ttttcgcctc actggcttag
    12841 gtcgataccc atgtggactg cctgcaacaa tgtatcttac ttttttgaca actgtttctg
    12901 ctggcaattt gccaatatct ttaattattg taccgtctgt tcctgctgtt ttactgcctg
    12961 catttgcttt gatatttcga tatgccagta atatattttc tctcgatagg ataatatcca
    13021 taagatgagt gaaaatatct ccattcttac ttttggcata taaatcatca aaggtgtttt
    13081 gcatttcgta atattcagcg tgtcttaaat cttctacaca caatgtttca tttttatttt
    13141 taggcataag gcttcactcc tttacttgaa attgagtgag cttttttatc ttactcgtaa
    13201 tccttattga aacaacataa tacttttcta atatatacac gacttgaggg cattcctcca
    13261 ttctcattac aagaatttct taggttcgcc ctctgctttt cagcattagt tcaactgctt
    13321 attgttcttc gtcagtttat tctcttgatg agaacccaat acctttcctc gttccgataa
    13381 tcttatctat acatatatat ccttaggtca ctagctataa gcctgctggc ttgataccgc
    13441 ctgtaacgtt atatggcatt tcataaccta tattcttact ttgccacacc agctacgatt
    13501 tctcgtacct ttgcatttct acaaagttca aatttagacc cgtacattcg ctgtttcgtc
    13561 agttccttat agaacattct aaccatagat attttataga cccccgacct gttggatacg
    13621 ctacccacct ctggatagtt ttcgatagaa gatatatgtt catcaactat tacggtatct
    13681 ttcagccgtc tttaccgagc ttcaaacatt ctgagtttcc tcatacaatg cttgtcggag
    13741 gctcagggca ggcgtttcag agcgttactt catcattcca cctgtcagat tatcagttct
    13801 cctaataacc aaactttttc gttcttttat tagtgcctta gctttt
//
LOCUS       DAJSVD010000071        13802 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSVD010000071 DAJSVD010000000
VERSION     DAJSVD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.073"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            39..557
                     /locus_tag="ON498_001899"
     CDS             39..557
                     /locus_tag="ON498_001899"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3940144.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            685..1368
                     /locus_tag="ON498_001900"
     CDS             685..1368
                     /locus_tag="ON498_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR3940145.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1677..1750
                     /locus_tag="ON498_001901"
     tRNA            1677..1750
                     /locus_tag="ON498_001901"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1711..1713,aa:Arg,seq:tct)
     gene            1909..2268
                     /locus_tag="ON498_001902"
     CDS             1909..2268
                     /locus_tag="ON498_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR3940146.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2391..4022
                     /locus_tag="ON498_001903"
     CDS             2391..4022
                     /locus_tag="ON498_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR3940147.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4084..4725)
                     /locus_tag="ON498_001904"
     CDS             complement(4084..4725)
                     /locus_tag="ON498_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3940148.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4911..4987
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5007..5315
                     /gene="rplU"
                     /locus_tag="ON498_001905"
     CDS             5007..5315
                     /gene="rplU"
                     /locus_tag="ON498_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3940149.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5342..5686
                     /locus_tag="ON498_001906"
     CDS             5342..5686
                     /locus_tag="ON498_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR3940150.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5699..5992
                     /gene="rpmA"
                     /locus_tag="ON498_001907"
     CDS             5699..5992
                     /gene="rpmA"
                     /locus_tag="ON498_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3940151.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6085..7233)
                     /locus_tag="ON498_001908"
     CDS             complement(6085..7233)
                     /locus_tag="ON498_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3940152.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7245..7556)
                     /locus_tag="ON498_001909"
     CDS             complement(7245..7556)
                     /locus_tag="ON498_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940153.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7609..8004)
                     /locus_tag="ON498_001910"
     CDS             complement(7609..8004)
                     /locus_tag="ON498_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3940154.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8040..8384)
                     /locus_tag="ON498_001911"
     CDS             complement(8040..8384)
                     /locus_tag="ON498_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940155.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8685..8906
                     /locus_tag="ON498_001912"
     CDS             8685..8906
                     /locus_tag="ON498_001912"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940156.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9033..9809
                     /locus_tag="ON498_001913"
     CDS             9033..9809
                     /locus_tag="ON498_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3940157.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9812..10000
                     /locus_tag="ON498_001914"
     CDS             9812..10000
                     /locus_tag="ON498_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940158.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            10002..10286
                     /locus_tag="ON498_001915"
     CDS             10002..10286
                     /locus_tag="ON498_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940159.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10307..10495
                     /locus_tag="ON498_001916"
     CDS             10307..10495
                     /locus_tag="ON498_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940160.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10821..11060
                     /locus_tag="ON498_001917"
     CDS             10821..11060
                     /locus_tag="ON498_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940161.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            11066..11752
                     /locus_tag="ON498_001918"
     CDS             11066..11752
                     /locus_tag="ON498_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940162.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11755..12585
                     /locus_tag="ON498_001919"
     CDS             11755..12585
                     /locus_tag="ON498_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR3940163.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            12602..13453
                     /locus_tag="ON498_001920"
     CDS             12602..13453
                     /locus_tag="ON498_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3940164.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13450..>13802
                     /locus_tag="ON498_001921"
     CDS             13450..>13802
                     /locus_tag="ON498_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR3940165.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEER"
BASE COUNT         4600 a         2253 c         2714 g         4235 t
ORIGIN      
        1 gattaagaag gattgaataa agaaagaagt tgaaaacgat acctgattac aaaaaaataa
       61 cttttataag ttctataacg ttgattatat ttttaattct tattcttctc gtcgtcttgg
      121 ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg agtccatatt
      181 atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt ttagtcggag
      241 atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg
      301 ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat attgagcctg
      361 gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca tatattggac
      421 tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg attgcttttt
      481 gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag cggagacgaa
      541 cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc caacgactct
      601 tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca gttgttcgta
      661 taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa
      721 gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga gctaggcaaa
      781 ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac agttgtattt
      841 ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg agcagctatc
      901 ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt tacgacaatt
      961 gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct aggactgaca
     1021 gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt gtctggtccg
     1081 tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc
     1141 ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca aggaggaacg
     1201 atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg taaattaaat
     1261 gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt gccattaggt
     1321 gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata catgattatg
     1381 gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt aaaagtttct
     1441 atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta gcatgaagtc
     1501 aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat gtctcgtttt
     1561 aaaagattga tttagtagtt gggccaaaat aattcattat ttgctaatta aataactatt
     1621 tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg attcttgtca
     1681 tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc
     1741 tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt
     1801 gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca
     1861 attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta
     1921 tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg
     1981 gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc
     2041 agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg
     2101 ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta
     2161 atatctattt tttttgttgc attatatggc agtttgacta gcgatcgttg gattttactc
     2221 aaacaaggtg ggctagctct gttggcattg ctttctattt tcttataaat atagaaaaaa
     2281 taagaattta tctaacagga aactttgtga aaaatattac tttatctcat gaaatatgat
     2341 attctaatga atggtatttt agaaaatttc atgaaaaagg agttagatag gtgttcaaaa
     2401 attatgtttc ctcactaaaa aaagaatttt caggttatga tctttcaaga tttcagaaag
     2461 atcttttggc tgggatcaca gtagctgctg tagcattgcc tttagccttg gcttttgggg
     2521 tatcaagcgg agctgatgcg gccgcaggtt tgattacggc tatcattgcc ggtatcgtta
     2581 ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta
     2641 tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt attgcttgct acttttttag
     2701 cgggtctatt tttacttgca gcaggacttt tccgattagg aacactgact tcttttatcc
     2761 cagcacccgt aattacgggg tttacttcag gtattgctat cattattgca ttaggtcaga
     2821 tcgataattt atttggtgtt cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt
     2881 atcaaacgat aggatttccg ataaacacag cctctttgat catgggaggg ctagtcattt
     2941 tagggatgtt tatctatcca aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta
     3001 tcattttggc aacagcagtt atgctattgt tccatttacc agtagcaact gtgggtaaga
     3061 ttccacaaac attgatcagt agtaatcgtt tgaatatggg agattttagc ttttctgcct
     3121 tgcaacaagt ggcagttcca gctattagca ttgctttatt aggaatgatt gaaagcttgc
     3181 tatgtggtgc ttcggctggc cgaatggcaa ataagccctt agatagcaat caagaattaa
     3241 tggcgcaagg aattggaaat ctgttgcttc ctttctttgg cgggattcct gctactgcgg
     3301 ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca aactagatta gctggtatga
     3361 tacatgcagt tgtcttactg ctctccatgc ttgtttttgc gccagtgatg tcacagattc
     3421 caatgccagc acttgcaggt gttttgattg tgacggcttg gcgtatgaat gaatgggaaa
     3481 cgatcaagga gctatttact aataaatatt ggtcagcagt tctattgttt atccttacga
     3541 tgatttgtac agttatcttt gatttaagta ttgctatcgt gatcggggtg atcggcggct
     3601 gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc attagaaacg atcgattggc
     3661 aaagaatggg gctgccagaa acggaaaagc tgaataactg gacagtagtg tatattagtg
     3721 gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc
     3781 acgatggtgt cattttctcc atgcgaggcg taccaagtat tgatttgacg gcacgtgcag
     3841 tttttgatga atttcaagaa aaagcagagc aaagaggaca aacgattatc tatacttcct
     3901 tgcagccaga agtagaaaaa caactttctc acttgtggga acatacattc acgcatcaac
     3961 atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa atatactgca attagccatt
     4021 aaaacaatga aaaagaacag acaagactgt aaactgaaga ttttatggtc ttgtctgttc
     4081 tttttaatat tgctgtaaaa gtgaagctag tacttgccag tcttgatgga taacaggtac
     4141 aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt ggaagaattc gatagttttt
     4201 ttctgaccat tcgtaggcat ccttttgttc tgttaattgt aagacaggcc ctgtatagag
     4261 ttgaccatga tttttggtaa ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt
     4321 tccaagtgtc gcaattaatt ttggctggac cttcatcagt tcgtaatcca atattggtgc
     4381 atgcgcaaat acttcggaac gagtaggtgt ccgatttgga taaacagttt ctgactgttg
     4441 tgtccgttta ttgatccgat gagtcacgcg atacgggcgg ctccttacag cactggtgat
     4501 atacacctct tctcgtgtta agccagttga agaaagtgcc tgcatcaatt cttttccagc
     4561 ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc cctggggctt ctcccacgag
     4621 catcagttta ggatgaagag gtccttgccc agcgataaat ccttctacag gacggccgtt
     4681 cattcttaaa gaagcttctt gtatcagttc ttttgggtat tccatttatg ctcctccttt
     4741 ttcttttatt ataagaataa aagaaacaca atgcgactat tttatttggt agaactgtga
     4801 ggatagaaaa gcagaagtat tgcttgaatt tttgttttac tttttgattt taaatttagt
     4861 tgactaacgg ttgctgttct ggtaagctag tactgttgta attgtggact ccacagctac
     4921 aaccgctcag aacaagtaat aagcacagca tatgctgtca cttcggatgg cgagtctaag
     4981 tatatcagaa ggaggtgctt tatagcatgt acgcaattat taaaactggt ggtaaacaag
     5041 taaaagttga agtgggacaa gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa
     5101 aagttgtttt tgacgaagtt atcttagtag gcggagaatc tacaaaagta ggcgctccaa
     5161 ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg
     5221 ttactttcaa atacaaacct aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat
     5281 atacaaaagt ggttatcgac gcgatcaacg cttaatagcg tttgagaaag aaggcctata
     5341 tatgatcaaa ggaacattta agcgaaatga cgcaggtcag atcgtttcat tcactttgac
     5401 cgggcatgct gatgctggtc cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt
     5461 agcgatcagc actgtcaacg ggattgcctc attagccggt tttgagccga ttgttgaaat
     5521 gaatgaagaa gaaggcggtt atctttatac cgaagtcact tcaggaatga cacaagaaca
     5581 aaacaatatt gctgagattc tattagaaaa tcttttatta ggattacaat cgatagaagc
     5641 tgaaaactca gaatatatcc aaattaaaac aattaatgat aaataggagg tgcagaccat
     5701 gttgttatct atgaatctac aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa
     5761 cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt gctgatggac aaacagttac
     5821 aggtggatca attctttacc gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg
     5881 tatcggtgga gacgacactt tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa
     5941 aggccgtaac aaaaaacaag tgtctgttta tccagtagct caagaagctt aataaataaa
     6001 ctgctctatc tcttgtgtag caagggatgg agcttttttg taaattatgg gatttttttt
     6061 gaccatactt ttgaccatac tttcttagaa gttaatataa tttgttagtt tttgtgttgt
     6121 ttcttctttt ttgctgttag taatgtgtgt atatgtgtcc atagtagttt ttatatttgc
     6181 gtggcccatt ctttcttgga cttctttgtg atctgcgcct gcctcatata gcattgatgc
     6241 atgtgtatgt ctaaaaccat gcagaccgat ataaggtaag ccggcacgct cacgatatat
     6301 attgtatctt tctgccactg actgattaat tgtgaaatta ttttcgtcat tggtaaacaa
     6361 ataattagct ttagtaaatc ctaattgaaa taagtacttt ctttgttcga gcttccaaaa
     6421 ttttagttgc tttatagttt tttcatctaa ataaattatt cgatttgatt ttttggtttt
     6481 aggagtttca gatatataat atttatctga acgagctaca gtttttttaa tggccatttc
     6541 atttgtttta aaattaatat tgtcccaagt gagtgctaat atttcgccga ttctacatcc
     6601 gctgaatgct aatagtctaa agagcgtata gtctctgttt ttaaaatatg gattctgttc
     6661 ttgagatact tcatttagaa ataattccaa ctgttctttt gtatataatt ttaatttctt
     6721 ttctgtttta atttgtggct ttggaataag taaatttaat gttggattat cagatgttaa
     6781 accaatattt atagcatact taaaaacttt attcatatag tttatgaaaa gagggtactg
     6841 ctttgaagtt cctttttcac tccaatcatt tactaccttt tgacaatatg ccgtattaat
     6901 tttagaaagc ttaatatttc caaatttggg atatatatgt ttattaaaga ttattttagt
     6961 ccttgaatat gaactttctt ttacagtttt tttgtagctt tctagccata attctgctgc
     7021 ttctttgaaa gtagtatttg tacttgtggg cattcctgtt ttttgtaatt ctagttctaa
     7081 cctagaaagt gctgtttttg cttctttttg tgttctaaat cctcgtttag tagtatattt
     7141 tttctttcca gttagcggat caataccgag ataagtttta aagtaccatg ctttttcgcc
     7201 atttttcttt ttatattgtt ttatcattgc catcaatatt acccctattc aaattctaat
     7261 tctgccatgc tatcattgaa aaattcgatc agtccttgtt cctctataaa ttcaagccat
     7321 atttccttcc cgttaattaa tggaactcct ttttctttac gaattgtttg atacaagtta
     7381 gtcacgactt gtttccacat ttctaataca gctttatcta ttttcttatc atcccagcgt
     7441 tttttttggg gtctattttt gtttttttca atttgataat tagtagcttt gatgataaat
     7501 gtttcgtatc cggttgtgct tttttctaac cattctttga attctgtata tatcattttt
     7561 gttcaccttt ctatttttga acgtatgttc ttttttgctt aaaaaatttt agagagctgc
     7621 ataacaatag atgcgagctt ttaataattc tttcacaagt ggttcgaagc gaacgcttaa
     7681 attgttatct tctataaatt tcatgtaatt gatattgtgt ggttctaatt cagactttgc
     7741 aatatatcta tctaataatt tttcaatcat gaagcgatca gcctcatatt ccatttttga
     7801 atggagagag aatgctaagt tatataaaaa ataattatca tgatgtttag aagcgtgtcc
     7861 taattcatgt aataaggttc tttgtcttct atacttagat aaattagctt ttattactat
     7921 ggtattcata caagcaacat aatggccatc ggcatcaagg ttttcacgct cttctacctt
     7981 aacgccgagt tcattaatta tcatttcaat ttgactgtcc aaatgactca cctacttatt
     8041 tagttatctt ttttctctaa atatgcttca atcatagctg atagtatttc cctatcatta
     8101 tcggtgagcg gttttccgtc gctactcata acagatgcta aagcttcttc cacggttagt
     8161 tttctttcct ctggtgcaac tcccgcgtta ggatcatctg tgcgtcctag caaataatct
     8221 gttgatacat ggaagtagtc agcaactttt tgcaaagttt ctattttagg actttgtgtc
     8281 ttccatttat aaatagagtt ttttccaaat cctattctat tttctaattc agaaatagaa
     8341 atacctcttt ttttacagag ctctttaacc ctttcgaaaa gcatgtcaaa tcaaccttcc
     8401 tagagcgtac gataataatt tatacaaaaa gatagaaagg tagttgacaa atctatccaa
     8461 ttgtcttata tttaactcgt aagctaattt aataagctaa caagcaacaa gaaatacacc
     8521 taataaaata aacgaattcg aggtcggcaa actaagaatg ttaattttta gggtttttct
     8581 aggcttattt aactgtatat aaatataaga cagttggata gaaatgtcaa taaaaattag
     8641 ctaattttat tagcttacat atttaaagaa aaggaggaaa catcatgaaa caaaataaaa
     8701 aaactccttc cgcaaaagga gctttaataa ctttaaattt taaagaccaa aagtttagtg
     8761 ctgttgttga tgaaattcat ccacaagagc atcaaaaaaa ttcttttgca actgacgaga
     8821 aaaagactca gaaacgctct gagaaagttc ttcgaataat tcaatttctg ttggaaaaga
     8881 ttcgtcattt tggaagtcta ttttgaattc tttattaaca gtcttgaata cggtttgttt
     8941 gaataaggcg tcgacatcca tattaaattc ttccatacta ttttcacctc gctttctatt
     9001 caaaattata tcaaagaaag gaatgagcaa aaatgaacac accgcaaatt tttaactttg
     9061 aacaacatga agtaagaaca gtaacaatac atgatgaacc attttttgtt ggaaaagatg
     9121 tggctaaagt tctagggtac caaaatggta gtagagatat taacagacac gtcgatgtag
     9181 aagatcggca aaactaccaa aacggtactt ttgaatcgcc tagagggtta actattatta
     9241 acgaatcagg tttgtactcc ttaattttag gaagcaaaca accaaatgct aagaaattta
     9301 aacgttgggt gactagcgaa gtgttgccag caattagaaa acatggaggt tatctaactc
     9361 cagaaaaagt agaagaagct ttgcttaatc cagatacaat catccaatta gcaactaagt
     9421 tgaaagaaga acgtactgga agactaatcg cagaacaaaa aattgcagaa tacgaaccca
     9481 aaatttcgta tttggatagt atcttatctt ctacagattc agtaactatt agtcaaatcg
     9541 cggctgatta tgggatgtct ccacaacaga tgaacaaact acttcataag ctaggtattc
     9601 agaaaaaagt aggcaaccaa tggttattat gcaaaaaaca catgagacag ggttacacaa
     9661 aatctcatac aactgagatt ccgaaatctg atggcggaac aaaagttgtg atgaatacaa
     9721 aatggactca aaaagggcgt ctgttcattt atgagtcgct gaaaaaagaa ggatatattc
     9781 cagaaatcga tttattagag gaggaatgac gatgtcacaa gaatttattt tgaaagtcag
     9841 aattcaatta gcaaaatacg gaaagtctca gaactggtta gctgatacta tcggaatttc
     9901 tagaccatat atgtcggaca ttatgaatgg gcgtagaaaa ccagataaac aaatcaagcc
     9961 aattgaggca gcactagcag agttagagaa ggagaaataa catgcaaata acaattccag
    10021 ataatttagt agtttccgaa ttaactacac agattacgaa tgctgttctt aattcattgg
    10081 acgaacgatt acaccttatg aacaaatcag tagagcttcc tccatatcca aacaaatcag
    10141 aggtaaaaaa agttttaggc attggtgatg acaaattaac acattggata agcctaggct
    10201 taaaaacaca gcagtggagc aagttagaca tcagaattga acgatcggaa ctccaaagat
    10261 ttttgaaaga aaactttgag ttctaaaggc aaaggagaat gattttatgt cctacacatt
    10321 gcaacaagaa catcaaattc taggtctgat taaacagcgt agaaaacaat tacaagatga
    10381 tcgtgcagcg cttagaaaat ccgatgagct atcagataga caagctgaac taattgcttc
    10441 tgaacttgag gatttgagaa tgctagaaat aaaaaatagg gagattagat tatgaagaag
    10501 acagacacac tttttatagg attcattttg gggttattag tgattgtagc gcaccaaagt
    10561 attatcggag gaagcttgtt cgcagcattg atggttttaa tcaatctgct tgactcaaaa
    10621 gaaaggagca actatggcac gagaagaagc gctaaaaatc ggtaaagtga ttgctgataa
    10681 ttggtgggca aatagccgtt ctattatttt aagcaagcaa catatcgaaa agaaaaaagc
    10741 atggcaacaa ataaaaaagt gactccgccg accaaagcaa tgagtcactc aaaaaatcat
    10801 aactaaggag attttaacat atggaaaatg aactttccac tctagatcaa tatttggccg
    10861 atcctgattg gggcaaatcg aatttacaac tggttgctaa caagtacgaa aaatcaattg
    10921 atgaagcaat tgttgcattg actgatgcag cttccataac cgctgatgac agtaaatgga
    10981 ccaagagaga cttgggcatt ctaaaacgag gcgttaattg gcttgaagat cagtacagag
    11041 aagaaacaaa agagaagtga tatgagtgtt taaaccacta atagattcat actcggcggt
    11101 actaaagaaa tttaaaggta acgacattgg tgcaacaatc aacgaagaag taaatatcga
    11161 acggctgaaa acgatgtatg acggatatga tggcgatcga gtcattgaaa ttcgctttat
    11221 tgatccacga cggttcacag tacagcaaag aaacttcatc tatgcgctca taggcgatat
    11281 tttcatcgat acaggcatgc caacggactt ctggaaggaa ttcttctact tccgttttga
    11341 aggtgtcaca gggcgcgaaa taagcctcaa agacgaatcg aatacaaccg tgagtgatgc
    11401 caatatctta gcgaatatca tcctagattt catctttgaa catcatattc ctttcaaaga
    11461 aggatatgag attttaccag cgaatcaaga gtattacttc tacaaatgca ttacaaaaag
    11521 agtctgctgc atctgtggca aaacaggagc tgatatcgat cactttgaca aagcgctggg
    11581 aagacgaaag cgcaaagaag ttgatcattc agagtacaca tttgcagcac tctgcagaat
    11641 ccatcacaca gagaaacaca aaataggtgt gatcaatttc aaaaataaat atcaaatcaa
    11701 agggatcaag ttaaaccagg aaacaattaa aaagttaagg ataggaggat aaatttggct
    11761 gagataagtt ggatcaaact taaaactact atgtttgacg atgaaaaaat acgattaatc
    11821 caagctgttc ctgagtcgga tgccatcatc gttatatgga ttcgattact agttttagca
    11881 ggaaagacta acgacgatgg tctgatatat atccagagga acatgcctta taccgaagaa
    11941 atgcttgcta cattgtttgg caaaaacgta aatacggttc gcttagcgct aactacattg
    12001 gcaaatttca acatgattga tctaagcagt gatggactaa ttgccatcag taattgggaa
    12061 aaacatcaaa atatcgaggg tatggataaa gtaaggctaa aaaatgctga aagaaaccgt
    12121 aaatacaggg aaagaaagag acaggaacgt ctcaaattgg aaaatgacgt tagcgtgacg
    12181 tcacgtgacg gtacagataa agatatagaa gaagataaag atatagataa agaagaaaag
    12241 aaaggtaagt attctgacga acacttacgc cttgctaaaa agttgcaaag taatttaact
    12301 gaagattttc caaaagaaat gaacaaagta gatatcgaaa aatgggcaga cacaatcagg
    12361 ttgatggaag aaagagataa agcatctata gaagcgattg agtatgtgat caattggcta
    12421 cctacaaatg aattttggtt tggaaatatt agaagtgcta agaaattgag agaaaaattt
    12481 gagaagctca aattcgaaat caaagcagac aagaagaatc ataaaaagca aagtcaaaaa
    12541 ctacagtaca gcaatcctag tgaatatgac gacttgccaa tttaaaaagg agatgcatca
    12601 catggaaagc ctagcaaatg ctatggagaa actaataaga agagtattag tgcaaagcgg
    12661 aaaatgtcca gaatgtagcg aacctttgta tagttggcga gctaaaaata aggatggttc
    12721 agaacgttgt aaaccaacat gcatgagttg tggttataaa gcgttacgtg tgaaagagga
    12781 tatacagacc gaacggatat ataacgatag cttaaaagca cgagcgttga gtttttttca
    12841 aaatggttcg gtattaacag ataaaacttt gtttaaatgc aaaatggaaa attatcacgt
    12901 agtggatcaa gaaacaaaaa tcgctttaga aaaagcaaaa agctatacga atgaggttct
    12961 gctgaaccat cctgcacatt tcattctatc agggaaatca ggaagcggaa aaagccactt
    13021 atcaatggcc acagcttggg aaatacttga gcgctcaaat tatgacaaga aaatactttt
    13081 tataagctat caagaattat tagagcaaat aaagttttct tataacaata ctgaactgag
    13141 aaaagaaatt gaaggatcgc ttatagccga tattaaaaca actgatttgg tggtttttga
    13201 cgatattgga gctgaattag gtagtggggt atcaaatagt aggcagttta caaacaacac
    13261 gttaaacacg ctcttagaag ccagacagaa caaggcaacg atcatcacaa caaacttatc
    13321 tggtcctgaa ctaagagaag cctacggtga aagaattgtt tctaggatat ttaagaattc
    13381 agaaggttat gcgctgaaat tccaacaaac agcagacaag cgcataaaac cagtgaaagg
    13441 tagtatcgca tgaataaata ccgtaataaa aaaactgttc atcgaggtat caagtttgat
    13501 tctatcgcag aagcagagta ttacgatcta gccttgtggc aagctgaagc aaatggctgg
    13561 aaagtaaaac ttcaggaaag atttgagctg atgccgaaat ttgaactaga cggaaagaag
    13621 tatcgcaaga tcgagtatat tcccgacttc acattttata aaaacggcaa acttgtcaaa
    13681 gtcgtagatg tcaaaggaat gcagacaaaa gactttaaga tcaaggcaaa gttgttctgt
    13741 catcaatatc aagcgccgtt gatattagct aaaaaatatc ggaatacgtt caaggaagag
    13801 cg
//
LOCUS       DAJSVD010000072        13543 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSVD010000072 DAJSVD010000000
VERSION     DAJSVD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.074"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(163..387)
                     /locus_tag="ON498_001922"
     CDS             complement(163..387)
                     /locus_tag="ON498_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCR3940166.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(585..2165)
                     /locus_tag="ON498_001923"
     CDS             complement(585..2165)
                     /locus_tag="ON498_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3940167.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2566..3942)
                     /locus_tag="ON498_001924"
     CDS             complement(2566..3942)
                     /locus_tag="ON498_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3940168.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4070..5188)
                     /locus_tag="ON498_001925"
     CDS             complement(4070..5188)
                     /locus_tag="ON498_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3940169.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5330..5764
                     /locus_tag="ON498_001926"
     CDS             5330..5764
                     /locus_tag="ON498_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940170.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5842..6375)
                     /locus_tag="ON498_001927"
     CDS             complement(5842..6375)
                     /locus_tag="ON498_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR3940171.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6469..7647)
                     /gene="mutY"
                     /locus_tag="ON498_001928"
     CDS             complement(6469..7647)
                     /gene="mutY"
                     /locus_tag="ON498_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3940172.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7640..8437)
                     /gene="recX"
                     /locus_tag="ON498_001929"
     CDS             complement(7640..8437)
                     /gene="recX"
                     /locus_tag="ON498_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3940173.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8544..9926
                     /gene="rlmD"
                     /locus_tag="ON498_001930"
     CDS             8544..9926
                     /gene="rlmD"
                     /locus_tag="ON498_001930"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3940174.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10100..10678)
                     /locus_tag="ON498_001931"
     CDS             complement(10100..10678)
                     /locus_tag="ON498_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR3940175.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11000..11470
                     /locus_tag="ON498_001932"
     CDS             11000..11470
                     /locus_tag="ON498_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR3940176.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11578..12804)
                     /locus_tag="ON498_001933"
     CDS             complement(11578..12804)
                     /locus_tag="ON498_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3940177.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3983 a         2730 c         2255 g         4575 t
ORIGIN      
        1 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag aagcaactcc
       61 ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa ttccagctta
      121 tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt ttgatttttc
      181 tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt tatttttaac
      241 tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt ttttgtccca
      301 gtcttttaca tctaataatc gacataatcg taacatctct tcttttttct gtttttttct
      361 ttcacgtctt tgccattctg tttccatgtg tcacactctc ctttctttaa tcatatgtac
      421 gcaaaataat ttttatattt ttttgattag acatttctat gattttaata tagtaaaaag
      481 agccgtcacg gaaaatccgt cacggctcta aacaaaatat gagaggctgt ttttgtttgg
      541 ctattcttat tacttgatgc agggcagctt gtgtcaagac ttctttacat caaacttttt
      601 aattttacat tgggatattt atccgcaaac cagcgttctg caaattggtt ctcgaataag
      661 aacaacggtt ggtcgaaacg gtcacgagcc aagatattgc gacttgaact catcttctca
      721 tccaaatctt ctggatcgat ccagcgagcg attttgtgtc ccattggaga catgattact
      781 tcggcgttat actcatttaa catacgatat tggaatactt cgaattgtag ctgaccaaca
      841 gctccaatga tatattcttc cgttagatat gttttataaa gctgaatcgc tccttcttga
      901 actaattgat agattccttt gtggaaggat ttttgtttca tgacattttt tgcagtcact
      961 ttcataaaca gctctggagt aaatgaaggg agttcttcat aagcaacttt tagttttcct
     1021 tcgaacaatg tatccccaat ctgataattg cctgtatcat atacaccgat gatatcgcct
     1081 gctactgctt tttcgacgtt ttctctcgaa tctgccataa attgtgtgac attgcttaat
     1141 ttcagttttt tatttgtacg ttccaaccag acatccattc ctcgttcgaa tgttccagaa
     1201 cagattcgga caaaagcgat tcggtcacga tgggctggat tcatatttgc ttggatcttg
     1261 aatacgaaac ctgaaaattc ttcctcataa ggacttacca tctgatcatc ttgtgttttg
     1321 tgtccataag gagctggtgc caattcaacg aaagtctcta aaaaagtctg tacaccgaag
     1381 ttagtcaatg cagaaccaaa aaatactggt gtctgatcgc cacgagcaat tttttcttga
     1441 ttaaatgcat ctccagcctc tttgattagt tctacttctt ctaagacttg ttcatataag
     1501 gaattttcag ttagaggatg attttcggga agcagtccat cttcatccaa ttctactaat
     1561 cgttcatcct gataattttc cggacgataa aactctacac gattatggta gatatcataa
     1621 agaccttcta gccctttacc cataccgatt ggccaattca tcggataaga ttcgatatct
     1681 aatagctctt ctaattcttc taacaattcc aatggttcac gaccatcacg atctagtttg
     1741 ttgataaaag taaatatcgg gatgccacgt ttttttacaa cttggaataa tttctttgtc
     1801 tgtgcttcga tcccttttgc actatcgatt accatgaccg cactatccac ggccatcaaa
     1861 gtacgataag tatcttcaga aaaatcttca tgtcctggtg tatccaagat atttactcgt
     1921 ttcccttgat aatcaaactg cataacagaa ctagtaacag aaatcccacg ttgtttctcg
     1981 atttccatcc agtctgattt ggcaaaatta cctgtttttt tgccttttac ggtcccggct
     2041 tgacggatag ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt cccagcatcc
     2101 ggatgggaaa tgatcgcaaa agttctgcga ttgtccactt gttgctttaa ttttggatta
     2161 ttcataacct aactccattt cttccattca attatttata ttttctctag gcacagattg
     2221 tccgatctct taaagaaaga caatttcata tagggctgtg acaaaagccg tgtagcagtc
     2281 tctttttccg agtaaacggc gttctagaga cagcctatca gcaataagcc cacccaagcc
     2341 aaaaatacga aaagctgttt ttgcttggct gctcttatta cttaatgcag gatagctttt
     2401 tcacaacctt tgtcaactac tacaactgaa tcagtatagc atggattcag cgaatgaaaa
     2461 agccaccaaa ccataagagt caaatatttt tttgtcagct gactaaaaga aaagacgtgt
     2521 tgcttgttta ttcaagcaac acgtctttca taaccaagtt tccaattatc ttcttggctc
     2581 gtcatcttcg aattcttttc tgaagtatct tcgttcttct tcaggttccg cttctgtttc
     2641 ttccggatca tgaaaaatga cacggatttt aaggattcga gaaccttcca tttcttcaga
     2701 aaccagcgta atattatcga catcaaacga taacttttcg ccttcatccg gaatcatgcc
     2761 taatgctgta atcaaataac cagccatcgt atctacatca ctcatatgca gatcactgtc
     2821 aaaagcttca ttgaactcat caatcagcat acgtccttgg atgatatatt cgtgttcatc
     2881 gattttttca taaagattct caacttcatc tgattcatca tcgatttctc cgacgatttc
     2941 ttctaaaagg tcttctagtg ttgctaaccc aacgacgccg ccatattcat ctaataaaat
     3001 agccatctga ttctgcgtct ttttcatttc atataacaga tcatcaataa agaccgtttc
     3061 aggaacgaat aaaggctcct gcatgatttt cttgatatct aaattatcaa agccgaattt
     3121 atgggcagct tttagtaaat tctttgtatg aaggattccg actactttat ctttgtcttc
     3181 gttataaaca gggattcttg aatagttttc ggataaaact tcattcacat tttcttccac
     3241 agtgtcgttg atatcaatca taaatgcatc cgttcgtggg accatgactt ctcgggcaac
     3301 tttcgtatca agagaaaaaa ccccttggag catttctaat tcttcattat tcaatacgcc
     3361 ttcgttttca agcatataac gcatttcatc tctggtcatt tttgaatctt catcatcaaa
     3421 agtcatcggc gtgatacgcg ccaataaact tgtggaagca gaaagcagcc agacgaaggg
     3481 cttagctact acaccaatca agcgtatcag ccctgaagtg aactttgcga cttcttctga
     3541 tttatttaaa gcaatacgtt tagggtacaa ttctccaaaa acaatcgata cataggtcaa
     3601 aatagccagc acaatgatat ttgcaatatt ttttgccgct gtgccgccac ctaataccgg
     3661 agctaaccgt tccgataaag tattagctag tgaagcacca gacaaaatgt tgaccaaagt
     3721 gattcctact tggattgtcg ataggaaatt atttggattc tgcaatattt ttaaaagctt
     3781 ttgagatttc acatctcctt catcagcttt ttgctcgatt cgattcttat tgatcgaaac
     3841 aacagcaatt tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga tcaataataa
     3901 aatctgcgct aacagcgact gactctcagg gtcagcattc ataatcgcat tctctcctta
     3961 taaaattaat ttttagttta cgatagaaaa aaacgtggca ctatagccac gttaaaatgg
     4021 tatcacaaag caattttctt tgcaataaaa acaagtgtac taaaactacc tacgaggtcg
     4081 tagcaatttt cttcttgttt tcattttgct tgtcttcttt gacgattttg attatatagg
     4141 aaatgatcgt cttgcagatt gcgtaaaaag gaacacctaa gaagatacca agcaatccgg
     4201 caatatttcc agctactaga agaacgataa tgatcgttaa tggatggatt ttcagtgatt
     4261 tcccaattac attaggataa atgatattcc catccaactg ttggacgatt agcacaacca
     4321 cacaacaaag taatgctttt accggatcat tgaagactgt tactagaaat gcaggtgcta
     4381 gtcctagata aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg accccaaata
     4441 agaaagcata atccacacca attaccatat atcctaaaaa tgtgaaggta ccaacgaata
     4501 aacattcgat tgcttgtccg ctaatgtaat tagccaatgt tttattcagt tgccccaata
     4561 actccacaat ttggtttctt cttttttctg ggaaaaactg ttggatgttt ggcactagtt
     4621 tttctccgtc tttcaacata tagaacaaga tgaatggtgc tgtaatgatt actacggttg
     4681 ccgaagcgat cgttccaacg atcgaaccta aactattgga tactccacta aggaattgct
     4741 gaatgattgt cccgtaagaa atgtctaatt gctcaaaata tttattgata tctacttcct
     4801 tgaacaaagg caattcggct acctctttta accagctttc tacctgtttg atgaaatcag
     4861 gcatattaga tgccaatgag gaaatttgct gtactagact tgggatgaca ctaagtaaaa
     4921 tccaaataat tgctgcaact aaaaggagca gcacaattgc gacagcccat attcttttga
     4981 tttttgcttt catcaacagc ttgacaatag gattcaataa atagtaaaga aagcctgcaa
     5041 tcagtacagg tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa ataaagttta
     5101 tttctgatga aatcatgatc aatgcagcta tgatcaataa ttcaagcgac caaaacatta
     5161 gtttagactg tctaagtttt tcaaacaata gatttcctcc tttctttctt actagtatat
     5221 attatattcg ttaaaaattc ttttattctt tttttatttt tgaaaacttg ccatactttt
     5281 tatttgtttt attttccagt atactatacg aaaggaaggt ggctagacca tgaaagttgt
     5341 acaaacaaaa gatacaatga gcaatattta cttagatgct gtacggatca ggcatcaagt
     5401 ttttgtagtc gaacagggtg tgcctttgtc tcgagaaata gacaaagatg aagcccattg
     5461 tatccacttc gtcctgtact cagataaaaa agaaccgcaa ggaactgtac gtcttttgcc
     5521 tttagaaaac ggaaaaatga aattacagcg gatggcgatt ctttctgaat acagacacca
     5581 agggttagga aaaatattga ttgaagaagc agaaaacttt gctaagaatc aaggctataa
     5641 tacgatttta ttaggggcac agtccacagc tgaaactttt tacgaaaaac taggatatac
     5701 agcttacggc gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt
     5761 ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg
     5821 gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat
     5881 atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt
     5941 cacattttct tttaggatga agtcaatatc gttcgggtaa ttcatcattt tgctgtgcat
     6001 ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa
     6061 gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca
     6121 ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg
     6181 aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa
     6241 tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg
     6301 ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc
     6361 ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt
     6421 tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt
     6481 cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac
     6541 gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg
     6601 ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa
     6661 aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa
     6721 tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt
     6781 ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt
     6841 ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg
     6901 attttgaaga gctgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac
     6961 aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt
     7021 ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt
     7081 gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct
     7141 gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac
     7201 gttgccgtca actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt
     7261 atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc
     7321 aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc
     7381 aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt
     7441 aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg
     7501 aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt
     7561 tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc
     7621 tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga
     7681 tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt
     7741 taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat
     7801 attctatttc ttgatctgct tcttgcggaa gatcagctat cgcttgttgg atcacttctt
     7861 gagaaaagcc tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt
     7921 ttccgtgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt
     7981 caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca
     8041 tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac
     8101 tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat
     8161 ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc
     8221 gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct
     8281 tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta
     8341 ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag
     8401 ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat
     8461 tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag
     8521 aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc
     8581 ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga
     8641 tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa
     8701 ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag
     8761 tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc
     8821 aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag
     8881 caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata
     8941 gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt
     9001 tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc
     9061 gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct
     9121 atgatgaacg gacaaacagt ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa
     9181 ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa
     9241 tgatccgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg
     9301 cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca
     9361 ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca
     9421 atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta
     9481 atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac
     9541 aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa
     9601 atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc
     9661 tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta
     9721 cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt
     9781 atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca
     9841 agattgaata tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg
     9901 tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc
     9961 aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac tgtacttgtg
    10021 accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg
    10081 tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg
    10141 tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa
    10201 gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga
    10261 tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt
    10321 agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc
    10381 ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc
    10441 cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac
    10501 tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc
    10561 ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa
    10621 aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt
    10681 agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt
    10741 atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa
    10801 cagaagtaga cgagaaaagt acaaatagaa aacatgagaa tcaaatcata tgatcttttt
    10861 caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat tatcaatttt
    10921 tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta aactaatatt
    10981 atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat
    11041 aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg gaaaatatta
    11101 ctattctttg caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac
    11161 cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa
    11221 acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc
    11281 tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt
    11341 tattttcttg tcagtgctta tgtatcagtt gtggctgcat catcgcctgg caatgtggct
    11401 catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt
    11461 tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct
    11521 aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta
    11581 gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg
    11641 tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc
    11701 ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata
    11761 ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc
    11821 ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc
    11881 gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt
    11941 tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg
    12001 tttgattgtt tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc
    12061 taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc
    12121 atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg
    12181 accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc
    12241 attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt
    12301 attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt
    12361 tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac
    12421 accaccaaat gcgtaggcat gatttaattt atctaccgtt ccataaccta cgatattcgt
    12481 ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac
    12541 taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc
    12601 taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga
    12661 ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa
    12721 tctgattccg tatgctgata atccggcaat cagcaaaacg aataccccta acaaggttaa
    12781 tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc
    12841 tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat
    12901 ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat cataccatac
    12961 cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttatg
    13021 tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga
    13081 tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga
    13141 tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt
    13201 atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca ttaccatcat
    13261 cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga
    13321 attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc
    13381 caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa
    13441 ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc taaaaacttc
    13501 ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctt
//
LOCUS       DAJSVD010000073        13404 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSVD010000073 DAJSVD010000000
VERSION     DAJSVD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.075"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..218)
                     /locus_tag="ON498_001934"
     CDS             complement(<1..218)
                     /locus_tag="ON498_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3940178.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFK"
     gene            complement(697..1416)
                     /locus_tag="ON498_001935"
     CDS             complement(697..1416)
                     /locus_tag="ON498_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3940179.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1465..3111)
                     /locus_tag="ON498_001936"
     CDS             complement(1465..3111)
                     /locus_tag="ON498_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3940180.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3290..3880
                     /locus_tag="ON498_001937"
     CDS             3290..3880
                     /locus_tag="ON498_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3940181.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3972..5477
                     /locus_tag="ON498_001938"
     CDS             3972..5477
                     /locus_tag="ON498_001938"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR3940182.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5561..6913)
                     /gene="celB"
                     /locus_tag="ON498_001939"
     CDS             complement(5561..6913)
                     /gene="celB"
                     /locus_tag="ON498_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3940183.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6913..7431)
                     /locus_tag="ON498_001940"
     CDS             complement(6913..7431)
                     /locus_tag="ON498_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940184.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7447..7773)
                     /locus_tag="ON498_001941"
     CDS             complement(7447..7773)
                     /locus_tag="ON498_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR3940185.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7786..9765)
                     /locus_tag="ON498_001942"
     CDS             complement(7786..9765)
                     /locus_tag="ON498_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3940186.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9786..10100)
                     /locus_tag="ON498_001943"
     CDS             complement(9786..10100)
                     /locus_tag="ON498_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR3940187.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10268..10561
                     /locus_tag="ON498_001944"
     CDS             10268..10561
                     /locus_tag="ON498_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3940188.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10639..11751)
                     /locus_tag="ON498_001945"
     CDS             complement(10639..11751)
                     /locus_tag="ON498_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3940189.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12138..12317
                     /locus_tag="ON498_001946"
     CDS             12138..12317
                     /locus_tag="ON498_001946"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940190.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12511..12681
                     /locus_tag="ON498_001947"
     CDS             12511..12681
                     /locus_tag="ON498_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940191.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13105..>13404)
                     /locus_tag="ON498_001948"
     CDS             complement(13105..>13404)
                     /locus_tag="ON498_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3940192.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4245 a         2695 c         2280 g         4184 t
ORIGIN      
        1 aatttgaaat tttcccaacc cacccaagga ctttccctca agctagccaa gaacccatct
       61 gctgaaatat gaaaattttt gaatgctacg acattcgcca ttacaggtat gtaaaagaaa
      121 aaaatcatcc aaagcatacc cggtacagcc atcaggacaa gtgctttata acgccataga
      181 ttcataaaaa aatgattgaa cccctttcgt tttttcatct aaaaacccct ttcgattcat
      241 tcgcaacgta attatagcgc tttcaataat gagaataaat aaacaaagta tagggaaaat
      301 gaaacaaagt ataggagagg ttgtgaaatc agcgttttgg cctgagtatt agagaaaaaa
      361 acgggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
      421 tgaacaccgt ttatcagagg ttgtgaattc agcgttttgg cctgagtatt agggagatcg
      481 tgtccttact atctaactct gagtcacaag gaacaatggg gactgtaaca aaagtcttgt
      541 cacagtccct tattccgaac aaacggtgtt ccagaagtag cttctacgga aataagccga
      601 aatttcacaa aaaagccatc cgaaagtcag acggacaagt ctgcttttca gatggcgcta
      661 caactactct gcttattctt ttagctatcc attcaattat ttttctctca cttgttctag
      721 ctcttctttg gtcatgggtc gatattgccc tttattcaaa tctttaggca gccaaaattt
      781 tcccatacgg attcttttta aatataagac ttcttttcct actgctttga ccattcgctt
      841 aacttggtga aactttcctt catgcaaaat cagacggata attgaagttt ctgtctcttg
      901 atctacttga tcaatttgga gttttgccgg caatgtctgc tcatcgccat caataatcag
      961 tccttctgca aataaacgga tatcttcttc tgtcattata ccgctaacct cagctagata
     1021 ttctttctcc acatgttttt taggtgatag taactgatgg gcaaacaccc cgtcattcgt
     1081 taaaatcaag aggccctctg tgtctttatc taatcggccg actggaaaaa gatcttcccg
     1141 ataatcctgg tcttccaata aatcgattac ggtttgatca tgattatcct gagtagcaga
     1201 aatgactcct gcaggtttat gcaaaatgta gtaaaaatct ttttgatacg taatgatttc
     1261 tcctaaataa gtaaccgcat cttcgaattc atttacttgc gctttatcgc ttttcaccac
     1321 ttgtccattg acttcgatcg ttcctttttt gatcaatcct ttgacttctt tgcggctgcc
     1381 aagtcccata tctgctaaaa acttatctaa acgcatgcgt ttcccccatt tcaaaagagc
     1441 atcaatctag ctcttctgat tcacttattt tatttttaat ttatgtcgta aacgcgctat
     1501 tcctggtcct agtagtcttt ctgccaagcg gattttcaaa gctaaataac catacactaa
     1561 agcccctata cctgctacta caagaatcaa caatagagac tggaatttgc tatcttcgtt
     1621 taaaaaagca ccaaaaatca tctttgtgat ccacgaagcc aaaagcataa tcaaggtaag
     1681 aataaagatc agcaatgtcc tgcgccagac aaaacttggt ttaaagcggg ctacttcatg
     1741 gattctcttt aagatcaagg tacaagacac agcaaatccg atcattgtcg ccaataaagg
     1801 gccgtatact tcaaataaac gaatggctgg gtattgaaga aggagtttca ctagaaaccc
     1861 aataaccaaa taattaattg ccgccttgtt ttcaaacatt ccttgcagca tgttggaaac
     1921 tagcatatat agtcctagga agagtcctac aaaacttgct tggatcagta cattacttcc
     1981 tagagaatcc ggtgtataaa ataatgtatt tagcggatag gcaagtaaca taacaccaaa
     2041 tgtcgccgga aacataaaaa atgaaaacaa ctgtaaattt ccgcttgtca attttgccag
     2101 acctactcga tctttcaacg taacagcttc tgtaatcaac ggtaacccag tagacgcgat
     2161 cgatgttgcc aatgcaataa ctaccatcgt caacttatct ggattggcac taaaaatcga
     2221 aaaaagatcc agtaactgtg cattggaata ctcggtggat gcagacatga tcttcataaa
     2281 agtgaactga tcgactaatt tgaagatcgt tacccctgaa cccacaataa taaacggaat
     2341 cgcttcttta acggtatcta acaacaattc cttcgttgca atatttattt tgttttcgct
     2401 gtagtgaata aacgccgatg tatacgcttg atgtttcttc aaaaaataca aaaggacaag
     2461 aatacttgct agcataccga taaatgccgc gaaagttgat tgcgtgaccg ccgtcacata
     2521 gtcaccatcc atgactttca tgatgatgaa tgtagccaat agcatataaa aaatacgtgc
     2581 cacctgttct acaatctgcg acagcgcata aggcatcatt tcttgattgc cttgaaaata
     2641 ccctcgtatc acactcatac aaggaaaaac caatacagcg atactcaaag aacgcatcgt
     2701 tggaatcagt tcttctccgc caccagaagc atgagctagc caaggagagg cgatgtacat
     2761 gaacaaagca aacaacaggc ctagcccgcc catcatctgt aatgcacgga taaataatcg
     2821 tcgtgaagtg ccatattcat tcaatgaatt atagcgtgca gtttgttttg caatcgcagc
     2881 aggtattcct gctgtagaga tcagtagaaa caatgcatat atattgtatc ccatattgaa
     2941 taatccattg gcagccttag cattttcgcc catccaagca taccagggaa tgatatagac
     3001 agcgcctagc aatcttgaaa tgatattact ggcggtcatc caagcagagc ctcgtgccat
     3061 tttttcttgc ggtgttaatt gatctaatgc tggttttctt tgattatcca tcggtcagat
     3121 tccaactttc tacaaaacat tactatcatt ttaattggta aaacagaaac tggcaatcga
     3181 ttttatataa cttttataag aacaattgtt tttttgaatt cggtttcgtt tttttggtaa
     3241 agtaaagaaa tatacagcct tttatggcta acatttatag gagatatcaa tgacacaaga
     3301 aataaaaaaa ctacccgtta tatttttagg tcttttcttt atcacagttg gattgaattg
     3361 gttcttgctc cctcacgata tagcttccgc cggagtgggc gccatcggac atttagttga
     3421 aacggccttt tctatccaac gctcagttac tgtttggagc atcaatctta tgatgctgct
     3481 tctagccgct gtcctattag gaaaatcagt attttttaag actgtagcgg gaagtctgct
     3541 ttttcctgtt gttttagaga ttgttccaaa agtggaaatg atctcctcac atttcttttc
     3601 actgatttca ggaagtcttt tgttcagtct tggggtttac acattgtaca cagtcggggc
     3661 ctcaaatggc ggtataacga taccgccgat tattttccac aaattttttc ggcttccaat
     3721 gccgcaagga cttttactta ccaatagtct tatcgtcttt ttgaattatt atgtttttgg
     3781 attgttggag acactattcg ttctcctatc tattacagtc agttcattgt ttatgaaaat
     3841 actcatccgc ttgtcccctg tgtcaagaaa atttgaataa agctggattt tgtcatagcg
     3901 gaaacaaacg atatattaaa aagagtgtgc tataataaac caagaaattg aataaagcga
     3961 gggatcaatc aatgagcttc tctttagaag acatccgtca tttactatta aaagaaaatc
     4021 tattaaaaga atttgtttca acaagaggct ggcatcttga tgtaccagaa aaagctgaat
     4081 ttcttcagtt aagttatgat tcaagaaaag ctgatgcttc tactttgttt ttctgtaaag
     4141 gaatgaattt caaagaagaa tatttggatt cagcaatcga acagggtatt caatattatg
     4201 tatcagaaca accatacgaa aattcagcag taggaatcat cgtaacggat atccgtaaag
     4261 caatggcgct tcttgctatg gctttttacg actatccgca aaataaattg aaagtgatcg
     4321 gattcactgg aacaaaaggt aaaacaactg ctgcgtactt tacaaaagca atcttggatc
     4381 acacaacaaa taaaaaaaca gcattgcttt ctactatgaa tacaacgtta gatggcaaaa
     4441 catatttcaa atcccatttg acaacgcctg agtcattaga tttatatcgt atgatgtcag
     4501 aagcagttga aaatggtatg actcatctag tgatggaagt ttcttcacaa gcttacaaaa
     4561 cacaacgcgt atacggcttg actttagatg tcggaatctt cttgaatatc tcgcctgacc
     4621 atatcagtcc gatcgaacat ccgacatttg atgactattt ctattgcaag cgacaattga
     4681 tcttgaattc aaaaacagtt gttttaaatc atgaaagtga ctattttgat ttacttaggg
     4741 aaacagccga tctattccag attccaacga tcacttatgg tcgttcggaa gaaagcaatt
     4801 atcaagtgat ccgttcagat aaaggtactc acggttttac tttatcttct catgaagatc
     4861 gcttagctat ctgcgatact gcttatgata ttttgctggc tggaggattc aaccaagaaa
     4921 atgcagccag tgcgatcatt gccgcagcac taacaggtgc ctccagtcaa gatgcacaag
     4981 acggattaaa agaagctcgt gtaccaggcc gaatggatca attgatccaa tccaatggag
     5041 cgcatgtcta tgtggactat gcacataatt atctaagctt gaaaacattg ctggaatttg
     5101 ccaaaaatga acatccagat ggccgtgtaa ttgttgtatt aggcagccca ggaaacaaag
     5161 caatctcccg tcgacatgat tttggaaaag tgctatcaga aacagctgac atcgcctttt
     5221 tgacggcaga tgacccagca tttgaagatc cgaaaaaaat cgcggaagaa atcaatgaag
     5281 caattactaa tccagatttg atcgttcatt atgaaatgga tcgtcctgaa gcgattcgac
     5341 gcgctttagc agaaagcgct ccgcaagatt cagtcgttat tgcgggaaaa ggcgtggatc
     5401 cctatcaaaa aatcaatggt gtagatgaac cttatgaagg ggattatgca atcgtgaagc
     5461 ggttgattga agaataatgg tatttatata ctagtggttt cccatttcta agaaaaaaag
     5521 ggactgtgat tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag
     5581 ctaattctgc tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa
     5641 atgggaacca gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc
     5701 aatcaccacc actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg
     5761 caagcatcgg tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca
     5821 tcggagctaa gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa
     5881 tgattggttc attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca
     5941 gctgatcaga tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc
     6001 caccaacggt tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg
     6061 cgttagctgc catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc
     6121 catggatccc tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc
     6181 atgtattcgt caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg
     6241 ttcctagtgc aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg
     6301 ggatcagagc agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga
     6361 cccaatttct cttgacacac atacaataca attcgaccgc aataatcgac ataataatag
     6421 ctgtaaagat tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta
     6481 ctacagtttc tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca
     6541 attcagggat acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa
     6601 catttaattt ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa
     6661 agtaaataga taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt
     6721 tgaaaaaagt tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta
     6781 aaaccaaaaa catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga
     6841 cagcccgaac gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca
     6901 aaaaatcaaa cattagttat tctcctttcc taaatataac gctttttcat attgtttaat
     6961 acggatatac tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc
     7021 actggcaaaa ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt
     7081 tgccataaaa ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa
     7141 attaaagaca ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt
     7201 tcgataaagc tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg
     7261 taaactactt cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa
     7321 aaagaaaaag gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa
     7381 actaagttgc tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct
     7441 tcctttctat ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt
     7501 catcgtcgtc atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt
     7561 cccgctggca tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc
     7621 atttgcttgt cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa
     7681 tgcttcatgt atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac
     7741 ttgtagtgtt tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt
     7801 gataaattta ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt
     7861 tagctggtca actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac
     7921 ataagaaggc gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc
     7981 agaaggaatc aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg
     8041 gactgccact gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt
     8101 tcgcatttct cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg
     8161 aagaatttct tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg
     8221 ttcggtgtaa atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga
     8281 atgatagggt atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa
     8341 aaaaatactg acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga
     8401 aataatcagg tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact
     8461 taccactgta atatttttac cgaattcact gactacgcga ttctttaata gttgggcact
     8521 gccgtaaccc gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc
     8581 caatgccgcc atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt
     8641 tttcaaagcc ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc
     8701 tttctttatc tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat
     8761 catcggtgac aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa
     8821 atgaaattcc tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc
     8881 attttgattt tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc
     8941 ttcttcggga aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac
     9001 ttgatactct actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc
     9061 ttctcggagc cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc
     9121 cgaaactttt aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa
     9181 actgatgtct gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg
     9241 gtttccaaaa aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca
     9301 tgtcccttga ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat
     9361 ttcttgcatg tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc
     9421 cataaaaaga atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc
     9481 ggactcataa aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa
     9541 aaacaagtca aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc
     9601 aatgatctta cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat
     9661 tgtccgatca gataataaca attcagaagc taaacgttga ctggtgacat actccttctt
     9721 gtgatcaatc aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta
     9781 ttccatcata tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg
     9841 acatacgctt ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg
     9901 aatttatcca aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc
     9961 ttaatcatat tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt
    10021 tgcttaaaga aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa
    10081 ataatcaatg cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc
    10141 ttgtaagcgt tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc
    10201 atatcttgaa taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg
    10261 ggatttagtg agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa
    10321 atttgcagaa gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa
    10381 cgagaaatat gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg
    10441 ttggaaagat gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa
    10501 cgaactccgt aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta
    10561 aaaagataca catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta
    10621 ttttttattc gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag
    10681 tatttcagtc accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat
    10741 gcggatcgtt actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa
    10801 agaattaaaa actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa
    10861 tataccgacc cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat
    10921 caatgagcca atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata
    10981 gctagataca atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata
    11041 ctctcctgca acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc
    11101 ctgcgaatac atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc
    11161 tcgatgtttt ctgtaaagaa gaactgaaaa ccaacaaaaa aacaaaaata ataagctact
    11221 tgtttgtgta aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag
    11281 atatgaaaag ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa
    11341 tatgctgctt agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg
    11401 gacatacggt gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga
    11461 aatcttataa ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc
    11521 gtatggttta tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa
    11581 caatacgatg aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt
    11641 agaagctgct tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata
    11701 aaaatccacc tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag
    11761 gttgtaaata ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta
    11821 ttcatgtttt tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa
    11881 caactgagag aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag
    11941 acaaaaattt gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga
    12001 tcgttgctgc agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg
    12061 tagatggaaa taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact
    12121 ttgaataata taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta
    12181 aaaactgtcg aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt
    12241 caaatcatgt cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta
    12301 tttcgaaaag ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa
    12361 gctatttacc aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg
    12421 ttctcacgtt tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa
    12481 attaatcgag aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc
    12541 tgcactaatc ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag
    12601 ctcaccaaac atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc
    12661 acgcctttca aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa
    12721 gacccgactt gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca
    12781 actgatcgct gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat
    12841 taggcaaaaa acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta
    12901 ttaactagta ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt
    12961 tacttattta ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac
    13021 cccttctaaa atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat
    13081 gcttattcaa ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat
    13141 gaaaacaagt agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt
    13201 ttttggaaga tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc
    13261 agcaactttt ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt
    13321 gaaagttact ggtgtcgcat ccaattcata ccccgttggc gcttgagttt ctaccagctg
    13381 atatgctcct ggtgctaagc catc
//
LOCUS       DAJSVD010000074        13212 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSVD010000074 DAJSVD010000000
VERSION     DAJSVD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.076"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            147..1016
                     /locus_tag="ON498_001949"
     CDS             147..1016
                     /locus_tag="ON498_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR3940193.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ON498_001950"
     CDS             1225..2250
                     /locus_tag="ON498_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3940194.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ON498_001951"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ON498_001951"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR3940195.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ON498_001952"
     CDS             3333..5036
                     /locus_tag="ON498_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3940196.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ON498_001953"
     CDS             5038..7071
                     /locus_tag="ON498_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3940197.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ON498_001954"
     CDS             7082..7522
                     /locus_tag="ON498_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3940198.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ON498_001955"
     CDS             complement(7722..8849)
                     /locus_tag="ON498_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940199.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ON498_001956"
     CDS             complement(8852..9529)
                     /locus_tag="ON498_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940200.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ON498_001957"
     CDS             9793..11559
                     /locus_tag="ON498_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3940201.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ON498_001958"
     CDS             11574..12011
                     /locus_tag="ON498_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3940202.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ON498_001959"
     CDS             12024..13175
                     /locus_tag="ON498_001959"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3940203.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAJSVD010000075        13073 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSVD010000075 DAJSVD010000000
VERSION     DAJSVD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.077"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="ON498_001960"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="ON498_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3940204.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="ON498_001961"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="ON498_001961"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3940205.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..3432
                     /locus_tag="ON498_001962"
     CDS             3229..3432
                     /locus_tag="ON498_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3940206.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3533..4195)
                     /locus_tag="ON498_001963"
     CDS             complement(3533..4195)
                     /locus_tag="ON498_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940207.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4208..5710)
                     /locus_tag="ON498_001964"
     CDS             complement(4208..5710)
                     /locus_tag="ON498_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940208.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6258..7937
                     /locus_tag="ON498_001965"
     CDS             6258..7937
                     /locus_tag="ON498_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3940209.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8064..8543
                     /locus_tag="ON498_001966"
     CDS             8064..8543
                     /locus_tag="ON498_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR3940210.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8762..9442
                     /locus_tag="ON498_001967"
     CDS             8762..9442
                     /locus_tag="ON498_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3940211.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9450..10781
                     /locus_tag="ON498_001968"
     CDS             9450..10781
                     /locus_tag="ON498_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3940212.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10796..11758
                     /locus_tag="ON498_001969"
     CDS             10796..11758
                     /locus_tag="ON498_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR3940213.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11869..12207
                     /locus_tag="ON498_001970"
     CDS             11869..12207
                     /locus_tag="ON498_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR3940214.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12286..12993
                     /locus_tag="ON498_001971"
     CDS             12286..12993
                     /locus_tag="ON498_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR3940215.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4154 a         2313 c         2876 g         3730 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3421 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3481 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3541 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3601 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3661 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3721 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3781 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3841 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3901 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3961 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4021 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4081 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4141 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4201 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4261 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4321 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4381 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4441 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4501 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4561 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4621 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4681 tgataatacc ggctacagtc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4741 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4801 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4861 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4921 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4981 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5041 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5101 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5161 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5221 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5281 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5341 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5401 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5461 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5521 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5581 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5641 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5701 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5761 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5821 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5881 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5941 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6001 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6061 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6121 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6181 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6241 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6301 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6361 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6421 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6481 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6541 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6601 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6661 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6721 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6781 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6841 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6901 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6961 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7021 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7081 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7141 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7201 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7261 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7321 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7381 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7441 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7501 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7561 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7621 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7681 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7741 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7801 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7861 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7921 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7981 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8041 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8101 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8161 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8221 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8281 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8341 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8401 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8461 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8521 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8581 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8641 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8701 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8761 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8821 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8881 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8941 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9001 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9061 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9121 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9181 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9241 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9301 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9361 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9421 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9481 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9541 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9601 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9661 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9721 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9781 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9841 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9901 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9961 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10021 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10081 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10141 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10201 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10261 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10321 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10381 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10441 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10501 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10561 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10621 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10681 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10741 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10801 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10861 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10921 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10981 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11041 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11101 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11161 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11221 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11281 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11341 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11401 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11461 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11521 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11581 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11641 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11701 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11761 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11821 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11881 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11941 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12001 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12061 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12121 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12181 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12241 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12301 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12361 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12421 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12481 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12541 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12601 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12661 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12721 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12781 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12841 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12901 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12961 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13021 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ata
//
LOCUS       DAJSVD010000076        12820 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSVD010000076 DAJSVD010000000
VERSION     DAJSVD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.078"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            105..1148
                     /locus_tag="ON498_001972"
     CDS             105..1148
                     /locus_tag="ON498_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3940216.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="ON498_001973"
     CDS             1321..1545
                     /locus_tag="ON498_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940217.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="ON498_001974"
     CDS             1548..1805
                     /locus_tag="ON498_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940218.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2022..2897
                     /locus_tag="ON498_001975"
     CDS             2022..2897
                     /locus_tag="ON498_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940219.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2907..3701
                     /locus_tag="ON498_001976"
     CDS             2907..3701
                     /locus_tag="ON498_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940220.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3730..4848
                     /locus_tag="ON498_001977"
     CDS             3730..4848
                     /locus_tag="ON498_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940221.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4881..5201
                     /locus_tag="ON498_001978"
     CDS             4881..5201
                     /locus_tag="ON498_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940222.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5456..6145
                     /locus_tag="ON498_001979"
     CDS             5456..6145
                     /locus_tag="ON498_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HCR3940223.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6182..6349
                     /locus_tag="ON498_001980"
     CDS             6182..6349
                     /locus_tag="ON498_001980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940224.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            6369..7997
                     /locus_tag="ON498_001981"
     CDS             6369..7997
                     /locus_tag="ON498_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940225.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8048..8419
                     /locus_tag="ON498_001982"
     CDS             8048..8419
                     /locus_tag="ON498_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940226.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8504..8704
                     /locus_tag="ON498_001983"
     CDS             8504..8704
                     /locus_tag="ON498_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940227.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8838..10064
                     /locus_tag="ON498_001984"
     CDS             8838..10064
                     /locus_tag="ON498_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940228.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10914..12239
                     /locus_tag="ON498_001985"
     CDS             10914..12239
                     /locus_tag="ON498_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3940229.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            12232..12579
                     /locus_tag="ON498_001986"
     CDS             12232..12579
                     /locus_tag="ON498_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940230.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT         4976 a         1591 c         2553 g         3700 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagcta cttttttcaa
     1621 ataagtacac agccaaagag ttggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaaggaatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggat ttcaagctga caatgattct aaaaaagttg
     1801 aatagtttaa tagtcgccaa gaaaagtacc aggacgaggg gaaaggtaga ttacaaagga
     1861 aaacactttt aaataaactt cgcaaaagca tcaggttatg agtggctagg gctatacgaa
     1921 tgtgatgaaa acaaagggct ttaatgtcaa agaaaacgca ataggggggg aacaaaattt
     1981 ttaaaaaaga tatttatgtt tggtttaatg gaaaaaaata tatgactact aacaaaaggt
     2041 ggtcagagct agtacaaaat acagttaaaa aagtcatgcc agatggatat catttcaact
     2101 atatacaatg ctcacaaaca actgaaagta tttatttttc aatattaaat gagaatagac
     2161 tctattattt cagggtaagt tcacaccata ggaaaaagag agaagataat gttacgacat
     2221 ttttatcggg aaaatacaat ggcttcaaag agttaagtga agccattggt gagtatctaa
     2281 aaagtaatag atttgtcaga atttctgaca aacatatatt gatcttgtta ctttatttga
     2341 ataatagctt caaattgttt tggagagacg acactttcta ctatattgag aacggcatgg
     2401 atatttattc tggtatcaac ggcatattaa aacattatat taaggcgtta cttcgatatg
     2461 gggttcttta tcaagaagat ttaagtaacc aaattaagat tagtaattct ggaaagtcat
     2521 tactggcaaa atataaaaaa ttgctttctc ctatctacaa aggaatagat atggaagctg
     2581 taaattctga aactttattt aatttagttg ttgatgaaaa gtttgcgaat gaatttttta
     2641 aaagtaaaaa aattgaaaga ctaaataacc tgtttcctga aaatgcaata aaagcttatg
     2701 tagataataa taaatcacga ttatacaact ggtttattcc caaacggttg cgaggaaaaa
     2761 tcaaaattgg cgatcaagtg aaagtaatta atctaaatca tactaagatc gttaccgtaa
     2821 cggagttgta tatagctgaa aaagaagata ttacaaaaat gaaaccagtt attaaaatac
     2881 tagataacaa agagtgagtg gaatatatgg tagcaaaaaa gaatactcta aaagaagctt
     2941 ctgaaaaata tcaagatatg aaaagacgag gattaatacc aaaggacaaa aaaggtatat
     3001 ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt gttggacgat
     3061 atacagcaaa aggttatagc aggcgcttaa ggatcactgt tgaatatgga acgaatgaat
     3121 cagctacaga gctgaagcaa aggtacttag atacacgaag agaagcttca aaaaaatatg
     3181 atgatttcaa gagcagaggt ctaattccta agaagaaaaa ggggatttgg cgtgagtttt
     3241 tcaaagaaga agatagcaat cggaataagg gagacttcat tggacgatac acagcaaaag
     3301 gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt tcatgggaat
     3361 tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta ttggagttgt
     3421 tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa ggttttatga
     3481 tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg aaaaaagcaa
     3541 gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga aaccggagaa
     3601 ctgaatcgag tgagattgaa gattactatg atgcctttac acacgatctg ggagcctttg
     3661 tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa gccggagatg
     3721 agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa aattaaaaga
     3781 cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac gatcaaatat
     3841 cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt gggctatgat
     3901 aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag tacctccggt
     3961 agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata tgatttcaac
     4021 gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta tttgaaagca
     4081 aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt aagaccgtat
     4141 gaaaatttaa aaacgtatat gttctttttg gttaattttg aaggaacagc caaagaagga
     4201 acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa tcccaaaaca
     4261 gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga ttattatgat
     4321 attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg attactcaaa
     4381 gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa gctttataaa
     4441 aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga aggagaaaca
     4501 agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt agcaggcgta
     4561 tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata tgggctatta
     4621 aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga agagctgtat
     4681 aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt cgaaaaagct
     4741 ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc taaagatgaa
     4801 gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat actctttgga
     4861 aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta ggtgttatta
     4921 ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa aaagttgcac
     4981 gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta aaaggggatc
     5041 agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt gattataaga
     5101 aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa tgggaaggta
     5161 ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa aacagttctg
     5221 actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc attcaaaaat
     5281 aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa gggtgttgtg
     5341 atagacgatg atttcaagtt tgattttgac tagatttttt tgcaatgttt aagagtaaac
     5401 aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg aatgtatgag
     5461 aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa aaagtcgaga
     5521 acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata gtgaagttcc
     5581 aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt ttcatgtcag
     5641 ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt ctatctctca
     5701 atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg cttttgattt
     5761 tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg agaagctaaa
     5821 aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt tctactccga
     5881 agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt ctcataagtt
     5941 atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta aacttgaaga
     6001 agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg aatttgacga
     6061 acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg aacttagcta
     6121 tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata cgatcgcaga
     6181 tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc acgtaaacga
     6241 tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat taaaaaggtt
     6301 tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag gagagagtgt
     6361 gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa aaacagttag
     6421 atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag gtacagatac
     6481 taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa cccaaggcat
     6541 tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat atgatcaaaa
     6601 agtatagacg agataattca aaagttgaag acgttttatt caacggtacg gcaaaacatg
     6661 atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt aatcagacag
     6721 atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata agtagcgaaa
     6781 caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat aaaaaattga
     6841 tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta ttcaaaacag
     6901 gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca gcattttatc
     6961 cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt gaaattaaaa
     7021 agatgaaaga actattaggt ataggatttg taaagcccaa ttcaaaagtc actattgctt
     7081 cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat tcagatatag
     7141 atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg atgggtattg
     7201 atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat attgtgtatt
     7261 tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa attgactatt
     7321 tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt aaaaatgcta
     7381 aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca agtattagaa
     7441 aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg agtaaagaaa
     7501 acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt ttgaagcaag
     7561 agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt gaaaataaag
     7621 tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa actgaagagc
     7681 cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta gtgggaggac
     7741 atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg tttgatgtaa
     7801 gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt tttctatata
     7861 cagattactt gaatcatggt ctatattatc gtgttatgag tgaaatttct aataaagata
     7921 agatatttta tttggataat catgtgaata tgaacttaac aattaaacgt atttacgatc
     7981 ttttatcact tatataaaga gagaagtgat aaataggaca caaaatttgg aaagtaggta
     8041 agtcactatg agagaactaa ctttatcttc taaggaatta gaaaagaaaa ctgctttatt
     8101 aaacgaaata tttagtgctg attgtataat ccaaagttta aaagaaagtt cattttcaaa
     8161 actaacagat caggaaaaaa tttcttatat gctaagttta gataatctag gaactaaata
     8221 tgatgaatca gcaagtagcg ataagctttt gattgaatta gtatcaagtc ttagtagttt
     8281 tggaaaaatg aaaagattta atgtgatatt agaaattttt gcttggttaa tcccttcatt
     8341 tatttggttt ttttatattt tcatagttga atctgagtta atagccaagg tctctcttgg
     8401 actgttaatt ccgatataaa taggttcagc aatttacttt aataacttgt atacacgtat
     8461 tcaaataaaa aagcagttat tacatgaagg attaagtggt aatatgttaa aaagaattgc
     8521 atgggataaa gataataaaa cagatattga gcttaaagaa tataaataca attatttctc
     8581 gagatgtata gatagaaagt tactgtttaa aaaagaagga ttagaattta gttttgatga
     8641 agatgaattt gaaaaattag tgatggactg gcgtaagaat ttactggata cggtacaatt
     8701 ctaaaagcaa ttgaggtcga aattccgata cttgaatcat gatctatatt atcgtgttat
     8761 gagtgaaatt tctaataaag ataagatatt tcatttggac agtcatgtga atatgagctt
     8821 aacaattaaa cgtatttatg atcttttata tgtttataaa cattaatgag gtgctaaaat
     8881 tgacaacaat aactatgaca aaaaaaatag aagtatatgt tgatggatca caagaagaaa
     8941 gggattcctt ttttgatttt ttttggaaaa atagtagaga atatgtaaaa gcgttgaatc
     9001 tggtatataa acagctaatg tttgaagagt tgataaaaga tgcaattaaa gaaatggatc
     9061 aaggttatgc tgagagagaa gaaagtattc aaaatcggat tgaaaagtta gcatatactg
     9121 atccaataga tcaaaaaaaa cttgataaag caaaagctga attatctaaa ttgagaaatg
     9181 aaaaaaataa agaagcgaga gaaatattaa cgcaagcgat agggttgaag agtcaaacga
     9241 gaacacgaga tgtagcaaaa aaattagact ttgactttgg agattttgtt gattccgcaa
     9301 atatgaaagc tagtaaagat tttggcaatg attttatcgg aattgtgacc ggacaaagaa
     9361 cgcctagaat atataaaaat agaaacaaat atggattact accttttaga ggtcgagata
     9421 ttaaaatcca taaagatgat aagttttata tttatttacc taaaggcttt aggtttaaag
     9481 tgaatttagg tagcaagcca agaaaagcaa gagatgttgc ttcagtttta caacacatta
     9541 tggatggcga ttacaaatta aatcaaagta atcttatttt tgatggaaga aaattatttt
     9601 ttaatctaac aatttctttt gaaaaaagct ataatgaaat ggaattcaag aaaggtaatg
     9661 aattgatcgt aaactttgca gatttgtgta gcggtgaatg tttaattaat gataaatctg
     9721 taatgtcttt tggtgatagc caatatataa gaaacttcaa aagtaaaatt ttagcaatga
     9781 aatcaaaaga acaaagccga ggagtctttt ctaagggcgg tcatggtcga aaaagaaaat
     9841 tagatcaaga tcgatgggaa gcaataaaga aaagagaaag taattttgtt aaaacttata
     9901 ataatcaatg ttctgcaaaa atcgttaaaa ctgctattcg caataaagct caaattattc
     9961 gtatcattga accagataag gcttatggcg aatgggctta ctatcaattg aaagaacaac
    10021 tagtaaacaa aggaaaaaga tatgggatag aagtcagaag ttagtaaatt aaacaatcgt
    10081 tgatttggca acgaaatagt gagggaaaat ccactcactg aagggtaaac tccgtcaagt
    10141 tgtgtgacca gacggaagga cgaatgttgt tgaggtttta gaaccatata aaacagcatg
    10201 tttctcaaat ttttaggata agggaaattg ctagcagttt gttttagaac catataaaac
    10261 agcatgtttc tcaaatgagt actttaaatt aattgatggc gttcctgctt tagaaccata
    10321 taaaacaaca tggatttaaa acaagaagta agtcagttct ccttttactt tagtaaggaa
    10381 aaataaagta ttctcaataa aacttaaaat aagcaaacct ttgttttaat agctaatcga
    10441 agtaaaaccc aaagccatat gaacccctga atcagcgaaa gtttatgatg atttggatta
    10501 ctaatagttt aggaattaga tggtaaatct aaattaaaca atcgttgatt tggcaacgaa
    10561 atagtgaggg aaaatccact cactgaaggg taaactccgt caagttatgt gaccagacgg
    10621 aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatagttct caaattgttt
    10681 aaaatgattg tcaagttaaa aaaatctggg atcatataaa atagtatagt tctcaaatat
    10741 gattagaaac cctattaaag agcaatatta agccaactta cttaaatcgt ttaattaaga
    10801 acaataatct cttttagtca gacttttagt ctgacttttt tgtatttcca aaatttattc
    10861 tttacaaaaa cgaacatacg tttgtataat tgcttgtgaa tgggggaata aaaatgtttg
    10921 attacagtag agaaccggtg aatgactatt ttttaatcga catgaagtct ttctatgcta
    10981 gcgtggaatg tattgaacga aatttagacc caatgactac accgttaatt gtcatgagta
    11041 gatcggacaa cactggttca ggtctgactc tagcttcttc acctactgca aagaaaaaat
    11101 atcatattac taatgttagt agagctactg atttacctta tccattacca aaagaacttc
    11161 atattgttcc accacgaatg aacttatata taaaaaaaaa tatggaagtt aatcagattt
    11221 ttagaaagta tgtcgcagat gaagatttat tgatttattc gattgatgaa tcaattttaa
    11281 aagtgacaaa atccttaaat ttatttacta gttcaggaac aagaagcgag agaagaagag
    11341 aattagctaa aataatcaaa gatgagattc agaaagaaac aggtctaata gcaacagtcg
    11401 gaataggaga taacccatta ttagctaaac ttgcactgga caatgaagcg aagagtagta
    11461 aagaatttat agctgagtgg acttatgaga ctgtacctga aaaaatatgg aaaataaacg
    11521 agatgactga cttttggggc attggatcac gcatgaaaaa aagattgaat catatgggaa
    11581 ttatgagtat agaagattta gcaaattgga atccctacac aattaaaagt cagttaggtg
    11641 taatcggact ccagttatat ttccatacca atggaattga tcgtacggat attgctattc
    11701 caccagaacc aaccaaagaa aagtcttatg ggaattcaca agttctacca aaagattata
    11761 cgaggagaga agaagtagaa ctagtagtca aggagatggc agagcaagta gccattcgaa
    11821 ttagacaaca caattgcaaa actggttgtg tacgcttagc aattggtacg tcaattttgg
    11881 aagaaaaatc aggtttctcg caccagatga aaataccaat tacagataat acgaaagaat
    11941 tacaaaatta ttgtcttttt ttattcaata agttttatga ggggcaggaa gtacgacata
    12001 taggagtaac ttatagcaga ttagtataca cagattccct acaattagat ttatttagtg
    12061 atccaagaaa aaagattgct gaagaaaatt tggataggat tattgataag attagacaaa
    12121 aatatggatt tacagctatt atacatgctt catctatgtt agaaggtgca agaagtatta
    12181 cgagaagcac tttaatagga ggacacgcag gaggattagg aggtttgcat aatgattgaa
    12241 tataacgata gaggacgttt aaaatggtcg ggattttatt tgtctgatca tactgaaaga
    12301 atcaatgcag acaatgtaca acgtgctaat cataatttgg caaaagaaca aatgacgact
    12361 gaagagattg cgaaaatact gagaactgct gtcataaaaa atagacccgt ctgcattcaa
    12421 aaagaggaac gaaacgctga aggttactat ccagatgatg tagtaggaaa aattattgga
    12481 tatgacgagc ttggtgtatt tgtggaagaa gaaaaagttc actatgatga aattaggaat
    12541 gtttctttct cggaattatt aaaatggagt cagatatgaa cattattaaa aaatacgaat
    12601 tcggttatat tacgtatgaa cagctaatag aggaattatg gggatatggt caacaattaa
    12661 ttaatgaagt ggggattgat tgcttttgtt tctatattga atccggagca ggataccata
    12721 agaacagata ctatatattt ccatatgcac caaaatagtc tcttagatat ttttaaattt
    12781 tctccaaata ctttcaatta ttgtgaaata tttcccttta
//
LOCUS       DAJSVD010000077        12736 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSVD010000077 DAJSVD010000000
VERSION     DAJSVD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.079"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON498_001987"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON498_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3940231.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ON498_001988"
     CDS             complement(322..891)
                     /locus_tag="ON498_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR3940232.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ON498_001989"
     CDS             1077..1979
                     /locus_tag="ON498_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3940233.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON498_001990"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON498_001990"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3940234.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON498_001991"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON498_001991"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3940235.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ON498_001992"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ON498_001992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3940236.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ON498_001993"
     CDS             8547..8732
                     /locus_tag="ON498_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR3940237.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ON498_001994"
     CDS             complement(8794..9990)
                     /locus_tag="ON498_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3940238.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ON498_001995"
     CDS             complement(10160..11596)
                     /locus_tag="ON498_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3940239.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ON498_001996"
     CDS             11889..12698
                     /locus_tag="ON498_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3940240.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4010 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaat
//
LOCUS       DAJSVD010000078        12704 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSVD010000078 DAJSVD010000000
VERSION     DAJSVD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.080"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..2124
                     /gene="acm"
                     /locus_tag="ON498_001997"
     CDS             <1..2124
                     /gene="acm"
                     /locus_tag="ON498_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR3940241.1"
                     /translation="ITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKF
                     SFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFN
                     ENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVF
                     YYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGE
                     QKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQ
                     INFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGI
                     PNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLS
                     SNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWE
                     PVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGL
                     VVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRF
                     TNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFP
                     EKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKS
                     SFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2396..2881)
                     /locus_tag="ON498_001998"
     CDS             complement(2396..2881)
                     /locus_tag="ON498_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR3940242.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2971..3316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3361..4002
                     /locus_tag="ON498_001999"
     CDS             3361..4002
                     /locus_tag="ON498_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3940243.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4021..4656
                     /locus_tag="ON498_002000"
     CDS             4021..4656
                     /locus_tag="ON498_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3940244.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4674..5489
                     /locus_tag="ON498_002001"
     CDS             4674..5489
                     /locus_tag="ON498_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3940245.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5808..7244
                     /locus_tag="ON498_002002"
     CDS             5808..7244
                     /locus_tag="ON498_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3940246.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7433..9880
                     /locus_tag="ON498_002003"
     CDS             7433..9880
                     /locus_tag="ON498_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3940247.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10110..11177
                     /gene="rlmN"
                     /locus_tag="ON498_002004"
     CDS             10110..11177
                     /gene="rlmN"
                     /locus_tag="ON498_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3940248.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11370..12473)
                     /locus_tag="ON498_002005"
     CDS             complement(11370..12473)
                     /locus_tag="ON498_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3940249.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4333 a         2153 c         2703 g         3515 t
ORIGIN      
        1 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
       61 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
      121 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
      181 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
      241 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
      301 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
      361 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
      421 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
      481 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
      541 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
      601 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
      661 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
      721 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
      781 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
      841 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
      901 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
      961 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
     1021 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
     1081 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
     1141 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
     1201 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
     1261 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
     1321 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
     1381 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
     1441 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
     1501 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
     1561 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
     1621 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
     1681 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
     1741 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
     1801 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
     1861 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
     1921 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
     1981 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
     2041 aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc
     2101 ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa
     2161 tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt
     2221 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
     2281 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
     2341 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
     2401 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
     2461 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
     2521 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
     2581 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
     2641 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
     2701 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
     2761 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
     2821 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
     2881 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
     2941 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
     3001 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
     3061 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
     3121 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
     3181 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     3241 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     3301 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     3361 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     3421 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     3481 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     3541 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     3601 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     3661 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     3721 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     3781 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     3841 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     3901 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     3961 ataatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     4021 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     4081 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     4141 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     4201 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     4261 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     4321 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     4381 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     4441 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     4501 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     4561 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     4621 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     4681 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     4741 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     4801 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     4861 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     4921 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     4981 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     5041 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     5101 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     5161 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     5221 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     5281 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     5341 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     5401 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     5461 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     5521 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     5581 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     5641 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     5701 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     5761 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     5821 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     5881 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     5941 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     6001 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     6061 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     6121 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     6181 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     6241 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     6301 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     6361 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     6421 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     6481 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     6541 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     6601 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     6661 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     6721 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     6781 aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     6841 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     6901 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     6961 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     7021 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     7081 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     7141 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     7201 actggtacca aaaactgatt gaagaaaatg gagagaacct ttaattcaca tacttaaaac
     7261 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     7321 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     7381 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     7441 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     7501 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     7561 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     7621 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     7681 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     7741 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     7801 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     7861 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     7921 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     7981 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     8041 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     8101 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa
     8161 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     8221 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     8281 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     8341 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     8401 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     8461 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     8521 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     8581 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     8641 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     8701 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     8761 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     8821 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     8881 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     8941 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     9001 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     9061 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     9121 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     9181 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     9241 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     9301 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     9361 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     9421 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     9481 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     9541 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     9601 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     9661 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     9721 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     9781 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     9841 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
     9901 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
     9961 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
    10021 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
    10081 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
    10141 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
    10201 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
    10261 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
    10321 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
    10381 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
    10441 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
    10501 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
    10561 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
    10621 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
    10681 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
    10741 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
    10801 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
    10861 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
    10921 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
    10981 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
    11041 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
    11101 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
    11161 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
    11221 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
    11281 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
    11341 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
    11401 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
    11461 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
    11521 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
    11581 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
    11641 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
    11701 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
    11761 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
    11821 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
    11881 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
    11941 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
    12001 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
    12061 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
    12121 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    12181 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    12241 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    12301 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    12361 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    12421 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    12481 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    12541 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    12601 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    12661 taatgaattt ttgttcctct tagtatttct accgattccc tttg
//
LOCUS       DAJSVD010000079        12657 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSVD010000079 DAJSVD010000000
VERSION     DAJSVD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.081"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(287..649)
                     /locus_tag="ON498_002006"
     CDS             complement(287..649)
                     /locus_tag="ON498_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940250.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ON498_002007"
     CDS             complement(723..1103)
                     /locus_tag="ON498_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR3940251.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="ON498_002008"
     CDS             1167..2360
                     /locus_tag="ON498_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3940252.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2436..3020)
                     /locus_tag="ON498_002009"
     CDS             complement(2436..3020)
                     /locus_tag="ON498_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3940253.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3221..4219
                     /locus_tag="ON498_002010"
     CDS             3221..4219
                     /locus_tag="ON498_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3940254.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4331..4840
                     /locus_tag="ON498_002011"
     CDS             4331..4840
                     /locus_tag="ON498_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3940255.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4870..5529)
                     /locus_tag="ON498_002012"
     CDS             complement(4870..5529)
                     /locus_tag="ON498_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR3940256.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5531..6295)
                     /locus_tag="ON498_002013"
     CDS             complement(5531..6295)
                     /locus_tag="ON498_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR3940257.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6303..6980)
                     /locus_tag="ON498_002014"
     CDS             complement(6303..6980)
                     /locus_tag="ON498_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR3940258.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7017..8402)
                     /locus_tag="ON498_002015"
     CDS             complement(7017..8402)
                     /locus_tag="ON498_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3940259.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="ON498_002016"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON498_002016"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="ON498_002017"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="ON498_002017"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3940260.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="ON498_002018"
     CDS             9341..10198
                     /locus_tag="ON498_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940261.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="ON498_002019"
     CDS             complement(10279..10614)
                     /locus_tag="ON498_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940262.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="ON498_002020"
     CDS             complement(10681..12051)
                     /locus_tag="ON498_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR3940263.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..>12657)
                     /locus_tag="ON498_002021"
     CDS             complement(12129..>12657)
                     /locus_tag="ON498_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3940264.1"
                     /translation="RVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYT
                     HFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDL
                     DMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNE
                     VTDEYLENILKEQSP"
BASE COUNT         3763 a         2561 c         2119 g         4214 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt
     2401 cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca
     2461 atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct
     2521 actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga
     2581 gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt
     2641 ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg
     2701 tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat
     2761 tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg
     2821 ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct
     2881 tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga
     2941 acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa
     3001 tctagttgtg ggaatgccat attgtttcct cctaatattt gcatacttca taataataat
     3061 aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact
     3121 agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct
     3181 caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac
     3241 aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc
     3301 taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc
     3361 cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga
     3421 tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct
     3481 ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc
     3541 caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga
     3601 taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta
     3661 tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat
     3721 gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca
     3781 cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat
     3841 cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag
     3901 tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa
     3961 tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc
     4021 agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg
     4081 aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt
     4141 acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa
     4201 aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca
     4261 gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg
     4321 gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa
     4381 ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta
     4441 tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg
     4501 tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa
     4561 attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga
     4621 gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta
     4681 tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt
     4741 ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc
     4801 acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa
     4861 aacgtctatt tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa
     4921 aagaaaaaca gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa
     4981 aaattcttct ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc
     5041 gttgttgaaa aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa
     5101 aaaaccgaaa acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag
     5161 ccgatcattc ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa
     5221 cacagcataa tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc
     5281 agaaactggg aaatctggca agctgaggat ggttttgaag ggattattga gataattcat
     5341 taggtgatca aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg
     5401 caaatgtccg atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag
     5461 actgatcagt gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga
     5521 atttttcata ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag
     5581 gcaacccagg tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat
     5641 tattgcgtat cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt
     5701 ctttttgcag ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga
     5761 tgtagatcgg cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag
     5821 gaagaaggcc gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac
     5881 ctttttgaat agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc
     5941 ctaccactac atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt
     6001 caatcaatcc tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa
     6061 aatcgattgt tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact
     6121 catctgctag acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca
     6181 agaaaggtaa ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt
     6241 gtgtcccacg ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc
     6301 cactactctt ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt
     6361 tgttcttgtt tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata
     6421 accggttttt tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta
     6481 tggttttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg
     6541 atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg
     6601 attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt
     6661 tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt
     6721 tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt
     6781 tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct
     6841 gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt
     6901 tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt
     6961 tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat
     7021 ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac
     7081 aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga
     7141 tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact
     7201 tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg
     7261 cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct
     7321 aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc
     7381 ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac
     7441 agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct
     7501 acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct
     7561 acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca
     7621 tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt
     7681 tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg
     7741 gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat
     7801 cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg
     7861 agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc
     7921 aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg gtactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctc
//
LOCUS       DAJSVD010000080        12626 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSVD010000080 DAJSVD010000000
VERSION     DAJSVD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.082"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(89..289)
                     /locus_tag="ON498_002022"
     CDS             complement(89..289)
                     /locus_tag="ON498_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3940265.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(741..1061)
                     /locus_tag="ON498_002023"
     CDS             complement(741..1061)
                     /locus_tag="ON498_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940266.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1362..2480)
                     /locus_tag="ON498_002024"
     CDS             complement(1362..2480)
                     /locus_tag="ON498_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940267.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2951..4258
                     /locus_tag="ON498_002025"
     CDS             2951..4258
                     /locus_tag="ON498_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3940268.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTTGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            4377..5576
                     /locus_tag="ON498_002026"
     CDS             4377..5576
                     /locus_tag="ON498_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3940269.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5603..6871)
                     /locus_tag="ON498_002027"
     CDS             complement(5603..6871)
                     /locus_tag="ON498_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR3940270.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(7034..7681)
                     /locus_tag="ON498_002028"
     CDS             complement(7034..7681)
                     /locus_tag="ON498_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR3940271.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8089..8592)
                     /locus_tag="ON498_002029"
     CDS             complement(8089..8592)
                     /locus_tag="ON498_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3940272.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8715..9479)
                     /locus_tag="ON498_002030"
     CDS             complement(8715..9479)
                     /locus_tag="ON498_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR3940273.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9586..9861
                     /locus_tag="ON498_002031"
     CDS             9586..9861
                     /locus_tag="ON498_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3940274.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10578..11507
                     /gene="yidC"
                     /locus_tag="ON498_002032"
     CDS             10578..11507
                     /gene="yidC"
                     /locus_tag="ON498_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3940275.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            11640..12134
                     /locus_tag="ON498_002033"
     CDS             11640..12134
                     /locus_tag="ON498_002033"
                     /inference="COORDINATES: protein motif:HMM:NF017416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940276.1"
                     /translation="MEEAKISLDALVPKNHLVHKIDKILLFDFIYPIVESTYSTIGRP
                     SVDPVILVKLVFIQYLFRIRSMRQSIKEAETNLAYRWFLGLSLIDKIPHFSTFGKNYV
                     RHFRETDCLNRYLSLSLNKQLKLDLFMKKFCIWIQPILKRMRKNIFLQEKWYINKPFY
                     TKNN"
     gene            12131..>12626
                     /locus_tag="ON498_002034"
     CDS             12131..>12626
                     /locus_tag="ON498_002034"
                     /inference="COORDINATES: protein motif:HMM:NF013752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940277.1"
                     /translation="MKEKINEARIAEGKRPFGSIIHQEVVEGKISVADPESGYYVKTE
                     QEKQFAYSAHTVCDENGFVLDVMITPGNLHDSRMLVPQIERVKSCCGVAADAAYKTPW
                     NAKYLIDRKLRPIFPYTRPKRSKERFKKKDFYYDPYYNWYLCPNDQTLQFRTTTRDGY
                     KKYVS"
BASE COUNT         3870 a         2549 c         2274 g         3933 t
ORIGIN      
        1 aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa cgcccagaca cgcaaatgtc
       61 tgggcgttta ttttttagta taggttaatt aagctttagt aacgtttgtt gcttgaggtc
      121 cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa gttttgaatc
      181 cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct gcagtgatga
      241 atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata ataaaaatcc
      301 tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg gaagatgttt
      361 atttgaaaca agctaccaca gattaccgaa caaaaactac tcctatagta tatcataccg
      421 tttacaaaaa agctagagtt tttcgacttt ttttattaaa aaaagttttc tcagcgatta
      481 cataaagtga aaaaacgaac tttttttaaa ataaacgaca tattgttcag ttggtgtttt
      541 tttgctctga tgttcttctt taaactaaaa aaattattct ttctgactac ttatcggttc
      601 tgtttcaacg gtcgaggctg gggaagattc tttagtaggt ctgatgttct catggtattt
      661 ctgatagcct atgacagtga ccagaagaac aaggcaaacc aatacgatca acgagattag
      721 tttttctttt ttgctcatat ctatacctca taaataacag gatgatgtcc atcatatttc
      781 tgagtatgag taaaagcaaa agctgcagct gtagaatgta agaaatgcca gcaactttca
      841 taatcacagg tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga ccgttcttct
      901 gtttcaggaa tcgcagaatc aagatctgtt gtctgtacat aaacattttt gtacagacgt
      961 gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa ctggctgact tgttaaactt
     1021 agggatagaa acagcaaaca ccataacagt ctttttttca taaagacctc cttatcgata
     1081 agtttttccc aattaaaatc atatctcaag aacgcccgtt attcacattt tgttcataaa
     1141 tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca acctcttttt
     1201 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
     1261 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
     1321 tccttaatac tcaggtcaaa acactgattt cacaacctct tttaataacg aagaatcgct
     1381 gtcagactct tttcatctgg cgcatctttt tgatccaaaa taaacacttt cccgccatta
     1441 atgatcacat catctgctaa tgcattcaac acttgacgcc gatcatattc tgtttcagga
     1501 tcttcaccga aaccatcagc taagtttgac gtagcgataa ataaatgtga cacttttcct
     1561 tctttcgcag ctggaacgat atcaactaac tgatcgatga atttcttatc taagagttta
     1621 ttgtattctt ttgattcttg agctgcgagt tcacttgaca gcttcttcgc cccttcttcg
     1681 atatctctaa aggatagctg cgcaggagaa gcagaaatcg caacgtctgc acaataatag
     1741 ggatttttag caattttttt gaacatcgtc tgattttctg gtaatgcaaa taaataaatc
     1801 ggccattgtt caggattttc gaaagaatcc tttaagtacg tatcaacagc ttggtaatag
     1861 ttgacccaat cgatctctac ttcttcatct tttggattta cgccatggaa agttactcca
     1921 tctcgggctc cgcctcctcc attagaagag aagttcaaac cgccacctgt cagttcagat
     1981 cccaatgcag taacgacatc aaccggtgcc ccttctggta gagatacttc ttttacttgt
     2041 ttgttgctga cctcatacaa tttcatggag tcgcggttca atcccaataa ataataagaa
     2101 tagttgaact gcccattttt gataatagcc agtaaataag gaagatcact tacatagtat
     2161 tgatcgtcta ccgggatact caaacgatgg atataggtct ggtcagcagt tataatgacc
     2221 gctacactcg tagttgcact acgccaaaac gaaggatctc ctaacaaatt ttcaattttt
     2281 tcttggaacg gagaccagtc atgttcggaa tattttttct caaaacgttt ctttgcttct
     2341 ttcgcaaaat ttttgaattt gatctgatct ttttccacat cttgatgggc gacatgcgta
     2401 ttcagcatga tcgtgacgaa tggtccttta gcgccttctg aagtcaattg tgtcaataat
     2461 tctttatcct tttttgccat aaacgtaccc ctcctaaaaa aatcattcct ttgtttctat
     2521 ctaaagtcta ccacaacact atttttccat acaataaaac gtatctaaaa aaataaaacg
     2581 gtttctacgg ttttaaattt agctattttt ttaagttatc ttcataattt cctcaattat
     2641 ttagccccag tcttcaacta ttcttcatga aatgctgata aactaagaaa aagaataaaa
     2701 gaggtggatc ttatgcattt aattatgatt ttcagtttat taagtattat cagtttattt
     2761 gctttcagcg cttcccgctt attagatgcg gaaacacggc ctattcctgt aaaagtacgt
     2821 aaaaacgacc gctaaacttc cataacgcgc gttcttatcc taaagttttt tcagaaacat
     2881 tgaaactctc tcccctctct gataaaattt taatttgtag cgaaacaaat taattacaag
     2941 gagatcggat atgtccataa aaaaaacacg tctagtccta ttattggcta gtctcattct
     3001 ttgccttgct tcttttctac cagctgttgc tgtgcaagct gaagatacgt tcaaagtaaa
     3061 tgcaaaagca gcttttgctg tagatgcgca aagcggtaag attctttatg atcaagatgg
     3121 agaaaaaacc acggggatcg cttcaatcac aaaaatcatc ggtctttaca tcgtccttga
     3181 tcaagtaaaa gaaggaaaac tgtcatggga tgataaagtt tcgatttcag attatgcaga
     3241 aaatttgagt atcaccccag atttatcgaa tgttcctttg cataaagaaa acacctacac
     3301 tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc aatgcttcaa tggtggcctt
     3361 ggcagaaaaa atctctggca gcgaagctaa attcactgaa cgaatgaaag aacaattgaa
     3421 agattgggga atcaaagatg caacaatcgt taatgcatca ggattgaaca actcttatct
     3481 tggcgaaaac cgaccagaag gcactggaga aaacgacgaa aatcaaatgt ctgctcaaga
     3541 tgtggccatc gttgcccgcc atttgatctt ggatttccca gaaatactgg atgtatcaag
     3601 tactacgacc caaatgtttg gcgaaaatac gcaatcacca gtggaaatgg tcaactggaa
     3661 ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg gacggattaa aaactggtac
     3721 gactgagctt gcaggtgctt gtttcgttgg tacgatcact aaaaacgatc aacggatcat
     3781 cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg agtgcccgct tcaatgaaac
     3841 agaccgtttg atggactact gctatgacaa ttggtcagaa aaagagctcg gaaaagctaa
     3901 cgcaagtatt cctagtctaa aaaccattga tgtaaaagac ggaaaagaat caacagttcc
     3961 tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac gatatggata ctggcaaatt
     4021 aacgatcacg ccaacacttg acaagaagca agtcactgac aatgagatcc aagcacctat
     4081 ggataaaggt acaaaaatcg gtaccgctac gatcaccttg gcagacgata aattaggcta
     4141 tttagaagac aacaatgcac catcaacaga gatcattacg gataaaacag tggaaaaagc
     4201 gaatatattc gtgattggct ggcgacgcgt cgcagaattt ttcgacaact tgttttaatt
     4261 gaacaataag aaaaacatct ttccatcgga aagatgtttt tttgcttttt ctagtttaaa
     4321 taacagcttt tttcatctat tttcgttata ctgtataaga aaaaggagtt gaatttatga
     4381 atctaaccag agatctgatc tttttagttc tctatcttat tggggtattc tctatcgttt
     4441 attggcgaaa acagaagaag gatccttcct tgttctttta cggcagtacg tggacattta
     4501 cggtaatctt aacttatttt gtcgtgttgg aaatttttta ttcgaccttt tattttttga
     4561 ttccattgtt gttctttagt atctttgctt tttcttattt cacagaaaaa cgaaggctgc
     4621 tgaatggtct tttattcaat gttttcctca tcagtttcgg catctacttg tttgtcttgc
     4681 tgtatgaaac gcaaaatatt ttccttggcg gactgatcgc cttgatcacg attccattgc
     4741 tacttgtcct tttatttggt atttatggat tgattgtttt tcttttttgg aacggggtca
     4801 cagttttacg tagagaatcg cattcattag ctaatctttt aacgttgata ctagccattt
     4861 ttcttacact gtttcttgtt tttgactttt tcttgttaaa atatttgccg caatggataa
     4921 atgctttgtt tttctgcgtt cccctgatct tgatctatct atttatcgta ttctacaact
     4981 ttttgactgt ctcattcttg tatcagttca accggcctcg atacaatcaa gacttcatcg
     5041 ttgtgttagg cgctgggttg atcaatggag aaacagtttc tcctcttcta gccaagcgaa
     5101 tcaataaggc aattgctttt tatcgggctc aaagccgtgc tacattaaat ccgcccatct
     5161 tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc agaggccatt gcgatgaaac
     5221 aatatgccat ggaacaagga atccctgaaa gagacattct tgtcgaaacc aattcgacaa
     5281 ccactttgga gaacatgctc tattcaaagg aaatcatgga tcagcaaatg aaagggccat
     5341 accgcgcgat ctttagttcc aacaattacc acatttttag agccggtctt tatgcgtggc
     5401 aagccaaatt gaaagcaaat ggtattggtg caaaaacagc tttctactac ttgcctaatg
     5461 cgtttttaag agaatacatc gccatcttga tgatgaacaa aaaaagacat atgatcattt
     5521 gcggattgct tttcgcaggc tccgcattct tatcgctgat tactttactt ttctaaaaaa
     5581 acaaggtgtg acaaaacata tgtcacacct tgtttttttc tttcattctc ttaactattg
     5641 gcaaaaagag tatccctttt ttgaccgcta cttgctgggc agaataaata ccgctgttgc
     5701 ctgaacacat aaagctgatc aagcagatca taaagaagta tactgccgct tgtgaaccga
     5761 acaattcgat ccccatcaca aagcaggcaa tcggtgtatt tgtcgcacca gaaaatactc
     5821 cgataaagcc caatcctgca agaaaaggaa cagaaacatg aagcaaaggc gctagtgctg
     5881 caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc tcccccttga tatcccgcgc
     5941 ctaaagacaa aactgtgaag aataacttcc caataaaatc atagacatgt gcctgtccat
     6001 taaatgattc ttgaagtaac ggaagactca atccgagata acgttgtgtt tgcaaaatca
     6061 atgctgctgc cacaactaca gctccaccta tcgtatttcg ccaaacagga tgaggaatcc
     6121 attgtgcata tcgcgctttt aaaaagacga tcgaacggct gaatacccaa ccaatcaaac
     6181 caaaagcaat cccagcaaca aaaactttga agaacagcaa catactccat tcaggaatca
     6241 cacccatttg gtaatgtaga tgagaaacac catagctttc tgtcatgaaa tttgcaaaca
     6301 gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac tttcccgatt gccaacactt
     6361 ctaatccaaa taatgttcca gctaaaggtg tgccaaatac agaactaaaa cccgcactga
     6421 ttccactgat aatcaagatt tcccgttctg ctttatccaa tcggaacaaa cgtgctgtat
     6481 tatctgctaa cgatcccccc atttggactg ctgttccttc acggccaaca gaccccccaa
     6541 ataaatgggt agttatggtt ccaaacagtg taagtgtaat aagccgcaac gggatttttt
     6601 cttctccacc atttccttga tcgatcacta agttgtttcc acgactagaa agaccgccgt
     6661 gatacgcata gagataagca aaaagcgcac cactaactgg tagtagataa aggagccaag
     6721 gatgtgatag tcggatatct gtcacaactg tcaggctatt taagaaaaaa gcagacaagc
     6781 tccccatgaa tataccgaga atcgctgtga tcaccagcca cttcagcatg tacactaaaa
     6841 tgcgcattgg actcggtaat tgtttttcca taaaaaattc ctcctcaata aaaaaacgcc
     6901 tactaaagaa aggggctgtc cccagacttt agtaggcgtc attagctatc tttcgttagc
     6961 atttaaaggc gaacaccatc acctattcac ttccattaaa agatagcata tttttctgta
     7021 aaaaacaata aaattatcgt tctttcttta cacgttcagc aaattcatag aaaggagcca
     7081 atttcgttaa ctgttgatta cattggtcac tatcatgaca aatataattc ccctttttcg
     7141 tatacgtgcc atcagaacct gctttcgttg tagaaagaaa aagcgacaca ttcgatgttt
     7201 tctgacagat tgcacagatt cccttattta tcgttggtga taaagtaccg ctaatccctt
     7261 ttaatttttc atcttcgtaa tagaccatga attttttctg cgtacctgga tcattccagc
     7321 cgatgtacgt atgttcttgc aagtcgattt cattccaagc tggttgtttg agttttttta
     7381 cttttcggaa taatttctcg atttgtggtt tgctaggctg cataaacggc tgaacgattt
     7441 gcttcagctt ctccaatgct ctttctgctt gcgcttttgt cagttttacg tctaaaactg
     7501 tcgttaaaaa tgtatcgatt tctggcagtt cgtgatttaa tatgtctcga atcttttcag
     7561 ctgctagtgc ctgaactgtt tgtaccgttt ttggatcatt gacagatgag tacgcattta
     7621 ataactgttc tacttctctt tttataaaaa aatattggta tggttttatt gtttgttcca
     7681 tgattttaaa gtcctttctt ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct
     7741 gcaaagtatg tgttacctga agatcccgga ctcttttaaa taagatacgt tttgcatctt
     7801 atcatcaccc cctttaatgc ttattcttct atgtacatga ttcattcgat aaataaaagt
     7861 aaaccgtatc ttgaacagat agtcaactgc aaacagaaaa aaggactgtg acaaaagtcg
     7921 tgtcaaagtc ccttgtttcg aatgaatggt gttcaaaagc ttgcatctgc ggtaataagc
     7981 cgaaacttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
     8041 gaccacttct gtcacaacct caatatggtc tattattggt tttacctgtt aaatcgagga
     8101 ttctttccca aagtaacgtt gccatctctt acgcatactt gcagacatgg gctgagccgc
     8161 ttctttgact gcgcagtatt tgtccacgaa tgtcacaata tatccttctt tatacttagg
     8221 cggtgcgatc gttgcacccc acatgtgctt caaaatgata tctcgctcaa gatccgacaa
     8281 atcggtgatt ttttctgcat tctttacagc aattcttgga tggatatacg catgtgtccc
     8341 ctcgtcaaat ttcgtcgtac gccaatcata gtagaacaga tcatgcaaaa gtccagcacg
     8401 cgctgttgct ttagcattgc caccccattt ttttgcgagc agataactat agtaagaaac
     8461 actgatggaa tgctccaaac gagtggaatg aacatgctgt gtgtaattcg ctaatttttt
     8521 cactgcttct gtttctaaaa gatcttgtac ataagacata tattcttcgt cttcacgcca
     8581 tttttctgtc ataattatcg atctgccacc tttttgaatt tcattttctt ctgaaattga
     8641 tctaagtata ccaccagtgt tcggattatt ctagtcgctc tagataaaat agctaaagct
     8701 taatagttcc ttaatcataa agaaaaatga taaagaacga tccctgctgc gactccgaca
     8761 ttcaatgatt ccgcatttcc tttcattggg atatatacgt tctgatcagc cgaagacaag
     8821 atttcctgac ttactccctg accttcgttt cccatgatta tagcgaaatc ctctgttttt
     8881 tctactttgt ctaatgcatg tgcttcttca tttaattctg taccgtacac cgtaaaccct
     8941 ttgcctttca ttacagggat cacttctgcc aaatcttttt gtaacacagg caaatgataa
     9001 ttgcttccct gcatgcttcg cagcaccttt gtactataaa tatctgccgt tcctttacct
     9061 aataaaacac cagccatacc aaacgcatct gccgtacgga tcatcgttcc cacatttcca
     9121 ggatcctgta cattatctag taaaagccag cctcctgaaa aatctggcgt atcttcagat
     9181 gttaacgtca tcactgctaa gatcccttgc ggtgtaggta gatctgacat agcggtcatg
     9241 acctcatctg aaactagaaa agtagaaatc tctttgttta cagccagcca gtctccccat
     9301 tctttcatac ctctctgtgt cacaaaaagt tcttttattt ctgcatcaga cgacgcagct
     9361 tcttcgatca gatggaaccc ttccaaaaga tattctgcct tttcttctct gtatttcttt
     9421 ttatgtagtt tttttaattc tttgattctt ggatttttta ctgattggat ctctttcaca
     9481 ttcctcacct caactgatta tatcatggaa taggtgcttg agcttgtttc atcctaaaaa
     9541 aatcggtatc attgatttat aaaacaggaa agaaaggtga cgaacatgcg caaagttaaa
     9601 atgaatgtcc aaggacgtgt ccagggcgtg ggatttcgct atatgaccaa gatggttgct
     9661 gatcagttag gcgtgaccgg aacggtaaaa aacgaagacg acggctcagt cacaatcgaa
     9721 gcaatcggaa atgacgacat catccaaaaa tttatcgaag aagtcaagaa gtctccgagc
     9781 ccaagtggac gagtgcagta tgtggatgtc caagaagatc cgatgattga ggaacgaaag
     9841 aaatttgatg tggtgggata aggttgtttt tgaagcgctt tgcctcgagt attaaggaga
     9901 aacattttcc aaatagcttc tcatattttg aaatgtaatg acttaatacc gagaggcagc
     9961 tgataaaaca ccgtcaataa ggttgtgaaa aagctggtct gcatcaagca ttaaggactg
    10021 gttgtgaaaa atagctctcc atatttttca acaggctgga cttaatgccg tagatgcaag
    10081 cttttgaaca ccgtcgataa ggttgcgaaa aagctgatct gcatcaagca ttaaggacta
    10141 gttgtgaaaa agtcgcttag ttttgagtat taaggaaaat tttgtgaaaa tggctttcca
    10201 catttttgca taaatttgtc ttaataccaa gaaactgact tttgaacacc gtcgataagg
    10261 ttgtttttga ggcgctttgc cccaagtatt aaagacaggt agtaaaaaag ctgatctatc
    10321 acttgcagtg agttcatttg actcctctat ttcgggcaga ataaaaaaag tttttagttt
    10381 tttaaataag aaagaaacac ttggaatcac tcttttcttt ccaagtattt ctttcttgat
    10441 tttttgtctt ttttcaaaat ttttcgctac gaaaaagaaa gaatatttat atgaaatttc
    10501 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
    10561 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
    10621 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
    10681 caggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
    10741 agaatttcaa ttggtcttac ggctgggcag ttattgcaat gactgtgatc gtccgtatca
    10801 tcatcttgcc actaggtatc agtcaatcaa aaaaaacaat gatccaatct gaaaaaatgc
    10861 aagcgttaaa accacaagtt gaagcagcac aacaaaaact aaaagctgca acaacacgtg
    10921 aagaacaaat ggcagcacaa gcagaaatgc aacaagtata tcgcgaaaac ggattgagta
    10981 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
    11041 attttacggc tcgctacaca gaaggtatcc gtgaatcatc tttttacggc atcgatctag
    11101 gctcaccaag tatggtgctt gttgccattg caggggtcgc ttatctgctt caaggatata
    11161 tttcaacaat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
    11221 ctcctgctat gatcgttttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg
    11281 tagtgggtgg tgtgttcagt tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
    11341 gtttgaaagc acaagtggca gaagagttga aacaaaatcc gccaaaacaa gtcgtcactc
    11401 caagaaaaga cgtgacagaa tcggcaaagc cagctgacaa taaacaacca aaaaacagta
    11461 caaacaacaa aggccgcaac gcaggaaagc agcaaaagcg gaaataaaaa tatatgacaa
    11521 aaaactgtag acaaataaaa aaatttcttt gttctacagt ttttttgtta caatcaaaga
    11581 aaacgtcgct tttcgaggag gaatgtataa tgatgtccaa ataatcaatg gatagtcgaa
    11641 tggaagaagc caaaatttcg ctagatgcac ttgttcctaa aaatcatttg gtccataaaa
    11701 ttgacaaaat attgttattt gattttattt atccaattgt tgaatcaact tactcgacca
    11761 ttggtcgtcc tagcgttgat cctgtcatct tagtaaaatt agtattcatt caatatttat
    11821 ttaggattcg ttcgatgcgt cagtccatta aagaagctga aacaaaccta gcttatcgtt
    11881 ggtttcttgg gcttagttta atcgataaaa ttcctcattt ttcaacgttt ggtaaaaact
    11941 atgtacggca tttcagagaa accgattgtt tgaacagata tttatcatta tccttgaaca
    12001 agcagttgaa gctagattta ttcatgaaga aattttgtat atggattcaa cctatattaa
    12061 agcgaatgcg aaaaaacata tttttacaag agaaatggta cataaataag ccattttata
    12121 ccaaaaacaa ttgaaagaaa aaattaatga agcaagaatt gcagaaggca aacgtccttt
    12181 tggttcaatc attcatcagg aggtggtaga agggaaaatt agtgtcgcag atccagaaag
    12241 tggttattat gtaaaaacag aacaagaaaa acagtttgct tattctgctc ataccgtttg
    12301 tgatgaaaat ggttttgtat tagatgtcat gattacgcct ggaaatctac atgacagccg
    12361 aatgctcgtg ccacaaatcg aacgtgttaa atcatgttgt ggtgtagctg ctgatgctgc
    12421 gtacaaaaca ccttggaacg ccaagtattt aatcgatcga aaactacgac ccatttttcc
    12481 ttatactcga cccaaaagaa gcaaagaacg ttttaaaaag aaagattttt attatgatcc
    12541 ctattataac tggtacctat gcccaaatga tcaaacactt cagtttcgca caactactcg
    12601 agatggttat aaaaaatatg tatcta
//
LOCUS       DAJSVD010000081        12446 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSVD010000081 DAJSVD010000000
VERSION     DAJSVD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.083"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(12..818)
                     /locus_tag="ON498_002035"
     CDS             complement(12..818)
                     /locus_tag="ON498_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940278.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            956..2470
                     /locus_tag="ON498_002036"
     CDS             956..2470
                     /locus_tag="ON498_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR3940279.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2873..3856)
                     /locus_tag="ON498_002037"
     CDS             complement(2873..3856)
                     /locus_tag="ON498_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR3940280.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3861..4508)
                     /locus_tag="ON498_002038"
     CDS             complement(3861..4508)
                     /locus_tag="ON498_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3940281.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4593..5225)
                     /locus_tag="ON498_002039"
     CDS             complement(4593..5225)
                     /locus_tag="ON498_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3940282.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5241..5792)
                     /gene="coaC"
                     /locus_tag="ON498_002040"
     CDS             complement(5241..5792)
                     /gene="coaC"
                     /locus_tag="ON498_002040"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR3940283.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5789..6526)
                     /locus_tag="ON498_002041"
     CDS             complement(5789..6526)
                     /locus_tag="ON498_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR3940284.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6523..7512)
                     /locus_tag="ON498_002042"
     CDS             complement(6523..7512)
                     /locus_tag="ON498_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3940285.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7770..8312
                     /locus_tag="ON498_002043"
     CDS             7770..8312
                     /locus_tag="ON498_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940286.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8329..9414
                     /locus_tag="ON498_002044"
     CDS             8329..9414
                     /locus_tag="ON498_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940287.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9426..10232
                     /locus_tag="ON498_002045"
     CDS             9426..10232
                     /locus_tag="ON498_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940288.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10233..11060
                     /locus_tag="ON498_002046"
     CDS             10233..11060
                     /locus_tag="ON498_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940289.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11057..12130
                     /locus_tag="ON498_002047"
     CDS             11057..12130
                     /locus_tag="ON498_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3940290.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3891 a         2247 c         2567 g         3741 t
ORIGIN      
        1 tttagcatgt tttatcgttt gattttttta ggaataaaat tcttgatgaa cgtaaatttt
       61 tggtaatcgt ttcgactata gaggtaacca aatacactga aaacaactgc tccaataaaa
      121 ttaacaaata aatccttcat agtatccatg attcccacgt ctaagtaacc attttggatg
      181 attgttttat ctgttttacc atctgcattt tgactttcaa tgattgtccg agtgatatct
      241 ggaacatgat agacagtgtt tccgtcttta cttaactcca cactattgat ttgctggatg
      301 atcgtatctt tttgcatatc gaattgcagt aatttatctg ctgaaaattc cacaaactcc
      361 cacataacac caactgtcat cgaaaaacag aaggaaacaa ctgctagtaa taaaggagtc
      421 aagttcatgg tttttgtatt tttattgagc aaataaacaa gggaaaagcc gacaccccca
      481 gctaaaaatc cgctgaatgc atgtagaaaa gtatcccaca ttgaaaaata gccataaaaa
      541 tcacttaact ctcctaaaac tgttgaacta aaaataaaag ataacaaaat gatttctagc
      601 atatttggaa tctgtatctt taatatcttt tccagaaaat aagggatttc aaataaaatt
      661 aatgtcagta ataaaagcat tgtatttccc cagtgttggt tccagacctg atccactagc
      721 ataataacga cgatgatacg taataaaata tagattccgt ttgtgattct acctgcagtt
      781 tttgtactgt tcgtaaaaaa ggttttcaaa tgattcataa aataactcct tcatgtatat
      841 ccttaattat actaagaagc gtgtgtttac caaagctata tgacttaaag aaaaacagag
      901 ttgatattgt gaaaaaaatc acttaatata gtataaggag aaaaggggga tacaaatgaa
      961 aaaattagtt gctggtttat taacagccgt tgcacttctt gctatgagcg catgcggcgc
     1021 agatcagaaa aatacagcgc agacaagttc tagtacgaag gaatctgccg aagtatcatc
     1081 aggcgcttct gaacaagtat atacagatcc aaaagaaatg aaagaatctt atgatgtcgt
     1141 gattattgga gctggtggtg cagggatggc agcagctatc gaagcaaaag atgctgggtt
     1201 gaatccagta attttggaaa agatgcctgt tgccggagga aacacatcta aatcatcaag
     1261 cggtatgaat gcttcagaaa caaaatttca aaaagaagaa ggaatcaaag actcgaatga
     1321 tgccttctat gaagaaacgc taaaaggtgg tggaggaacg aatgataaag aaatgcttcg
     1381 atactttgtc gatcactctg ctgatgcggt cgattggcta gatcaaaacg gtatcaaact
     1441 ggacaactta acgatcactg gagggatgag tgaaaagagg acacatcgtc cgtctgatgg
     1501 ctcggctatt ggtggatact tagtggaggg gttattgaat aacgtccatg agcgggaaat
     1561 ccctattttt gtcaacgcag atgtgacaga cattaagaaa aatggggaaa cggtcaatga
     1621 agtgactgta catgttcaag gagaagaacc aaaagaggtc accggaaaag caatcgtagt
     1681 taccacaggc ggttttggtg ccagcaaaga atttattgaa aaatatcgtc ccgatttagc
     1741 tgattatatt acgacaaatc aagaaggatc aactggagat ggtatcacca tgattgaaaa
     1801 attaggtgga caaaccgttg atatggataa aatacaaatt catccaactg tgcagcagga
     1861 tgagggggtc ttgattggtg aggttgttcg cggagaagga gcaatcttgg tcgatcaaga
     1921 ggggaaaaga tttgtcaatg aaatggatac aagggataaa gtatctgcag caattacagc
     1981 attgcctgaa aaatcagctt atttgatttt tgaccaaggc gtgcgtgatc gtgccacagc
     2041 tattgaattt tatgaccaaa aaggatatgt cacaactgga aagacaatgg aagaattagc
     2101 agaaaaaatc gatttaccaa aagaaacact agcaaaaaca gttgatggat ggaatacgga
     2161 tgttgcggag aaaaaagatg ctcaattcaa ccgtgccaca gccatggaac atgacttatc
     2221 gtctccgaat tattatgcga tcaaaatcgc accaggaatc cattacacga tgggcggtgt
     2281 gaaaatcaat acgaacactg aagtattaga taaagaaaac cagccaatta gggggttata
     2341 tgctgctggt gaagtcacag gtggcttaca tggaaacaac cggatcggtg gaaattcagt
     2401 tgcggagatc attatttttg ggcgacaagc gggcatacaa gcagctcgat ttgcttcgga
     2461 aaatgaatga aatagaacgt aaataaaaag aggttgtgaa atcagcgttt tgagctaata
     2521 ccgagggtca gctgattgaa cactgtttag tgaagaggtt gtgaaaaagc tgccctgcat
     2581 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gttggactta
     2641 ttaccgcaga tgcaagcttt tgaacaccgt ttattcggaa aaagagcgtg ggacaaaagt
     2701 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagaaattcc
     2761 ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa tttcttctta
     2821 tttcttggag ttaaacgctt ctgtcccagc ctcttttttg atcagcatga ttttattcag
     2881 ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc catatattca tgcgcttgtt
     2941 tgactgcgct gaattggaat gtacggtcaa tgattggccg aatcttgcca ttttctagaa
     3001 aaggcccagc tttttgcata aaagtttgtg cgagctctgc tttatactga tcacttctag
     3061 gtgtcaataa tgtaccaagc agttggacac gtttcccaat caatacacca aggtctatct
     3121 caggcacgat ggtcccacct agcataccaa tcaagaccca acgtccatca acggctatag
     3181 aagctaaatt cttttcccag taagaagcgc cgatgaaatc caaaataaca tcgactcccc
     3241 gaccttcagt gatttttcgt acctcttctt gaaagtcttg ttttttatag ttgatcaatt
     3301 catctgcacc aagttcttta caaaaggcga gtttttcttc tgatcctgca gtaacgatga
     3361 ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc tgttcccaca ccacttgctc
     3421 ctgcatggat caagacagtt tcgccttctt gtaattttcc aagccagtaa agtgtttgat
     3481 aagcagtcaa aaaaacttct gagattccag cagcagaaac atagtctagc gtatcaggga
     3541 gaaggattgc acgatcggca ggcatcacag catattcggc ataaccacct agattgacta
     3601 atccgtatac ttttgtccca ggagtaaaag tggagatatc acttttattc tcaactacct
     3661 ctccagctac ttcgactccc aaaatagggt agggaggttt caaagagact gattctcttt
     3721 tcataatatc tgttcgattg acagctgctg tatggacttt gatcagtaat tcgccttgtt
     3781 ttggtttagg aacaggcact tcaatttgtt ctaatacatc tacagaacca ggatgcttga
     3841 ttgaaatggc acgcatggat ctattcctcc tttgcccatt cttggaatgc tgtattcaac
     3901 tctttgctac cttcagaaag tactagagtc gtacgagaaa tatccgtttc ttccactaga
     3961 gcggcaagta cagcagcaac tgtagagcga gtgatcgttt ttgatgcctt ctcattaggc
     4021 ttagttaatt gaatggaagt aacttcttca tcgttagtca atgtgactgg ttgaagaatg
     4081 acaaaatcta agttagttcg tttcagccat tcatctgcat aatgtttagt aatataataa
     4141 tctgtcatgg actcaggcca ttttgctggt tcatctgcat aaacagcact gaccatcaga
     4201 tagcgagaga catgcgcatt ttcagctgcg atcactgttt tgattgcgcc atccaagtct
     4261 acttgcagaa gattctttcc ttgagagccg gcagcaaaaa tcaatacatc gatgcctttg
     4321 aatgcttcgg tcattttttc cacggaccaa gttaaatcaa aagagacata cgaaacattc
     4381 tcttttttta ctaatgtttg ggaagcaaca tctctcaccc ctgcaaaaat ttcatgggaa
     4441 cttggagcta aatcttttat caggtgtcgg ccaatttgtc cgtttgcacc tacaacaaaa
     4501 attttcattt ggatcatcct ttcgacgtca aatctttcca ttcaatagta acaccaaaag
     4561 agaaaaaaat cagagatcgg ctacgatgac tatcagttag taagagaagg gtgcttgaag
     4621 tatcttaaca agacattgca tactgctgca gtcgcaagac ctgcgacgat tccttcaggt
     4681 ataccacttg taaaaataac cgctagaata gctgaataaa cagcatttaa atttgttccg
     4741 atgactttgg cataatcttg ctggaataaa aagtagatca tattcatcac taaaatcgta
     4801 ttggtcaatg aaccggaaag tccagctaag aataaagaaa cggaatctgc tttttgtttc
     4861 attagtttgc gcagaccttt ataaacgaaa taaggaacca ccccaatcaa gatgcgagga
     4921 ataaacgcga tcagcaaggc tttccagctt ccatgttctg ttccgatgac tgggataaac
     4981 ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc tacggatcat gctgatcagt
     5041 ccaaatgtac cgccaagaaa agcacctagt cttggtccta aaacgatcga agcaatgatt
     5101 acaggaatat gcatggttgt tgcattgatt ggaccgatag ggataaaacc taaaggcgta
     5161 acagctaata aaatcaaaat agctaaaaac atcgcagtta atgtaaagtt ttttgtattt
     5221 ttcataagta ctcctttttt ctaagcattt ctttcattaa gagtcagcat gacctgttcg
     5281 atgatcgtct gaatatcagc taaggcacct tttccaaaat ctccacaagc taaaagagat
     5341 tctcttggtt caatttcctg atagccgaca gatctcaatg tttccagatt acgctgagtg
     5401 atcgggtttt gatacatatt cgtgttcatg gcaggggcaa tcaattttgg tgttcccact
     5461 ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa tgccgtttgc tagcttgccg
     5521 ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag cttgttttgc tagttcgata
     5581 tgattgatta cctgcggatt tgattcttgc atgacgtctg tatggactgc gcgcttcgac
     5641 agagattgca aggttaatgg cgtgataaaa tgtgtgctgc ttttagacat aatcacatct
     5701 acctgggcac ctaatttgac gaattgactt gtaagatcag cgcttttgta agcagaaata
     5761 cttccagtaa cgcctagtaa gatctttttc attttgattc ctccagtcta attttctcag
     5821 taatcaatgc agcaatctca gatttacttt gagcttcttc cactgttcca tctttagata
     5881 agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa atcatttgcg aaaataaaat
     5941 cagttttgtt atttatcaga ctattttttg cgactgccag aagagattcc tttggtacat
     6001 ctactaatag tttgaaacca atgatttttg cttttggctg ccattcacgt agataagaaa
     6061 tgatttttgg tgttttcttc aaaacgagca caagatgctc ggtgttagaa gagatctttg
     6121 aaacagtggt atctgtctgt tcattttccg caaaccaatt gtccagttct tcttgcgttg
     6181 aagaagtggg taggtttttt gccagctgtt cttctgaaaa actaaaagca ggagtaaaat
     6241 cactgactgc catactgtgg ataatcgcat cgtagtgttc ttttttcgtt agttcttcta
     6301 attgtaaaaa taagtccagt gcagattcga tcgtataaat cgttatattg gagtgtcttt
     6361 tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt ggtatccgga ttggcagcta
     6421 aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg atttgtaata gcacgtactt
     6481 gatcgatttt ttcagtcgtt cctccagctg tgattagaat cttcatttat ggtcacctac
     6541 atttactagt gttcttcctt ggtgtgttcc agcaagtagt ttagatgctg tttcaggcaa
     6601 ttgatccaga gtaatctctt ggacgaccag ttcatctgca atctgcaaat ccgccatctg
     6661 ttgccataaa gaaagacgtt gatctattgg tacattgaca gaatcaattc caatcaactg
     6721 gatgtttcgc agaataaaag gcaagacagt cgcgtttagt ttgattcccc cagcattgcc
     6781 acataaagct actgcaccgt tataagagat caatggaaga agactgctta gctgctcgcc
     6841 acctactgtg tcgatgacat agtcgatttg ctgttttcct aaagcttttg ttgtctctgg
     6901 cagaaattca tcaggtcgta cgacttgcaa agcacctaga ctttttagcc atgtgacaga
     6961 ctcttctttt ctggaaaagg caatgattga ctggaatcct agtttatgta acagggctat
     7021 cgctgtactg cctacaccgc cagaagcacc agtaacgaca acttttttat cggaggtcat
     7081 accttgacgg attaaagcgt tcacacacaa catagcagta aaccctgctg taccaaggat
     7141 catcgcttga cgcagcgtca tattttttgg tagaggaact aaccagtctc ctggtacttt
     7201 ttgatattgg ctataaccgc ctggatgggt gacgccaagc ccaaaaccag tagcaagtac
     7261 aagatcttct tttgaaaaac gtggattgtt actttggacg acagttccgg ataaatcaat
     7321 ccctggagtc atcggatatg ttcgtatcac ctgaccagac tcgctcattg ccaatgcatc
     7381 tttataattg acatcagaat aggcaatttt taccaagact tcattttcag ccaactcagt
     7441 aaatggcaga tcactgattg ctgtatggaa aacaggttcc tttattacct gaaaagatcg
     7501 aaaagcgttc attcgtgaga cactcctttt aaaattttct tttatcatac cataaagcct
     7561 actcttttat tcttgttctt ccgtaaattt agacaaaaat taggtgaaaa ataaagaatc
     7621 aaacaactag aatttattcc aaaagataaa gaaatattca gcaaaaaaaa ttttacaaag
     7681 ggttgccttt ttcaataaat ttcgtatact aatacgagtg gtagtaaaca ggaagtttac
     7741 tattagaaaa cttcgattcg gaggaaatta tggaaattgg tgagaagtta agaaatttgc
     7801 gcatccaaaa gaatttgaca caagaagaac taggcgaacg cacagatttg tcaaaagggt
     7861 atatttccca gttggaacga gatttaagtt caccatcaat ggaaacattc ttttctattc
     7921 ttgaagtctt aggtgtgact ccagaagaat tttttcatca agaagcagcg aatctgcaag
     7981 tggtttattc aaaagaagat catacggttt atttcgatga agaaaacggg tatgaattgg
     8041 aatggctcgt ggctgattcg aatgaaaaag aaatggagcc tgtcttgctg acttttgaag
     8101 gtgcaggaga atataagacg tttgaacctt cattatctga aacgtttatt tttgtattag
     8161 aaggggaatt agagttaagc ttaggagaga cggtctatgt ggcaaaagca ggacagtcga
     8221 tctattatca agcgacaaaa caacatcaat taaggaatca tctcaaaaaa cgaacaaaag
     8281 tattgattgt tgcaacgcaa tcatatttgt aatatagggg gtaaaaacgt ggggaaacca
     8341 atcatttcat ttgatcaagt agtgaaacgc tatgatgatg aaacagtatt aaaaaaagta
     8401 agttttgaga tcgaacaagg aaaattttat acactgcttg gtccttccgg ctgtggtaaa
     8461 acaacgatcc tacgtattat agctggattc accgaagcta cagaagggga catctatttt
     8521 gaagggaaac ggttgaatga tttgccagct aataagcgac aagtaaatac agtcttccaa
     8581 gattatgcac tttttcctca tatgaatgtt tttgataacg ttgcttttgg attgaagatc
     8641 aagaaaatgt ccaaatcaga tatcgaaaag aaggtaaaag atgctttgcg tatggtccag
     8701 cttcctggat acgaaaaacg ggagattagt gaaatgtctg gtggtcaaag acagcgtgta
     8761 gcgattgcac gagcaattgt aaatgaaccg aaagtattgc tattagatga accgttatcg
     8821 gcgcttgatt tgaaactacg tacagatatg cagtatgaac tgcgggatct gcaacaacga
     8881 ttaggaatca cgtttatttt tgttactcat gaccaagaag aagcgttggc aatgagtgat
     8941 gagatttttg tcatgaataa agggaaaatc gtccaaagtg gtacacccgt agatatttat
     9001 gatgagccaa tcaatcattt cgttgctgat tttgttgggg agagcaatat cgtggacggt
     9061 gtaatgatcg aagataatct agttgagttt gttggtaagc aatttgaatg tgtcgatggc
     9121 gggatgcgtc taaatgaacc agttgaagtt gttttacgcc cagaggattt gacgatcacg
     9181 acaccagata aaggaaaact agtcgtaacg gtcgatactc agctgtttcg tggtgtccat
     9241 tatgaaatca tctgttatga tgaacagcaa aatgaatgga tggtccattc aacgaaaaaa
     9301 gctaaagaag gaagcaaagt aggattggcc tttgagccag aagatatcca tgtcatgcgt
     9361 ttcaacgagt ctgaagaaga attcgatgca cgtcttgaca gctacgaaga atagggggga
     9421 caagaatgaa aacaatgcgc aggatctatt ccgtccctta tattttgtgg ctcggtttat
     9481 tcgtgatcgc gcctgtatta atgatcattt atcaatcttt ttttgatatg aatgggcaat
     9541 tcacgctgaa taattatcta tcttatttta cgtcggggac atcgctttct atgacgatca
     9601 attctgtatg gtatgcattt ttgatcacgt ttttcacgct gatcatcagt tacccgactg
     9661 cttatttttt aacaaaactg aagcacaagc aattatggct gatgctgatt attttgccta
     9721 catgggtgaa tctgctgctt aaagcttacg catttattgg gattttcagt gtgcacggca
     9781 gcgtgaatca tttctttgat tttatcggga tcgggccgaa acagatatta ttcacagact
     9841 tcagttttct gttcgtggca acgtatatcg agattccatt tatgattatg ccaattttca
     9901 atgcattgga agagatgaat ccttctttga tcagtgctag tagagattta ggcgcgaata
     9961 gctttgagac cttccgacga gtgatttttc cgttatcatt aaatggtgtg aaaagtggtg
    10021 tccaagcagt atttattcct tctctttctt tattcatgct gactcgtctg attggcggaa
    10081 atcgggtgat cactttagga acggctatcg aacagcattt cttggttaca caaaactggg
    10141 ggatgggttc tacaatcggt gtgattttaa tcattgctat gttcatcgtg atgctcctga
    10201 caggcgagaa gaaaaaagga ggtcgctcat aaatgaaaaa atttaaatgg tcgtatcttt
    10261 atttagggat cgtcttttta ttcttatatg caccgatctt ctatctgatt ttttattcct
    10321 tcaatgaagg aggaacgatg aacagcttta caggatttac ttgggaaaat tatgaagctg
    10381 tttttgaaga tacacgtttg atcattatcg tattgaatac gttcatgctg gctttcttat
    10441 ctgcattgat cgccacgatc atcgggacat ttggggctat gggaatctat tacacgaaaa
    10501 aacgacgtgc acggacagcg ttgttgagca tgaataacat cttattagtc tctcctgatg
    10561 ttattatcgg tgctagtttc ttgattttct ttacactact aggattcgga ttaggttttc
    10621 agagtgtatt gctttcccat attgctttca gcattccaat cgtcgtcttg atggtcttgc
    10681 caaaattgca ggaaatgaac gattcaatgg tcgatgcggc tagagacctt ggtgctaata
    10741 atttccaagt agtcaaaaat atcattttgc cttatctttc tccaggaatc atagctggat
    10801 attttatggc atttacctat tctcttgatg attttgcggt tacatttttc gtcacaggaa
    10861 atggctttac gacgctctca gtagagatct attctcgtgc aagacaagga atcagtttag
    10921 aaatcaatgc gctaagtgcg ttagttttct tgttctccat gatattggtc attggatact
    10981 actttattag taaagaaaac cgtccaaaac ggctgaagaa aaacagaaga gtacgcgagg
    11041 gggtggcacg gctgcgatga aaaaattaca atctctgttt ttaggtattc tcatcattat
    11101 tgcggtgctg ctgcttggtg ttcgtcaact tgaaaaaaca agtggcatga gtggagcaaa
    11161 ggtacttacc atctataact ggggagatta tatcgatcct agtttgatca cgaaatttga
    11221 aaaagaatat ggatacaaag tgaattatga aacatttgat tctaatgaag cgatgttcac
    11281 aaaaatccag caaggtggga caaactacga catcgctata ccaagtgaat atatgatcca
    11341 aaaaatgatc aaagaaaaac tagtacttcc attagatcat tcgaagatca aaggattaaa
    11401 taatattgat ccacgatttt tagacttgga ttttgatcca gataatcagt attcgattcc
    11461 atatttttgg ggaactttag ggatcgtata taacgataaa ttctttgatg caaatcaaat
    11521 taagcattgg gatgatttgt ggaagccgga attaaaagac agtttgatgc tgattgacgg
    11581 tgcacgagaa gttatggggc tttctttgaa tagtttaggc tattctttga atagtaagaa
    11641 tatggcacag ctgacagaag cagcaaataa attgaatcat ttaacaccaa atgtcaaagc
    11701 aatcgtggca gatgagatca aaatgtatat gatcaatgaa gaagcctccg tggcagtgac
    11761 attttctggc gaagctgccg atatgatgtg ggaaaatgaa catttacatt atgtgatccc
    11821 accagaagga tccaatcttt ggtttgataa tatcgttatg ccaaaaaccg tgaaaaataa
    11881 agaaggtgcc tatgatttca tcaactttat gctagaaccg gaaaatgcgg cacaaaatgc
    11941 agagtatatt ggttattcta caccaaatga agaagcaaaa gcgttattgc caaaagacat
    12001 ttcgtcggac gaacaattct atccatctga tgacacgatc tcccatcttg aagtctacaa
    12061 agatctagga tcgaaatacc taggcatcta taatgaccta ttcttagaat tcaagatgta
    12121 taggagataa tggcaaaact cagtaagaat ggggacttat caacgttcta aagagctaaa
    12181 aatcgtcctt tttctatctg agttttccaa tcttctacca aaagcggatg aaacgccgct
    12241 ttttttgtgt tattttattt tttctaccac gtttttctac cagagaaaag tggacaaaac
    12301 cattgtaaaa tagggcccga gacgggctag taaatctaag attgaaaaag ttacagaagg
    12361 ataaagtaat tggaaactat gcaaatatac ttaacccttc tgtaatctat taataaaact
    12421 aatatatgaa tccagttcaa tatttg
//
LOCUS       DAJSVD010000082        12319 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSVD010000082 DAJSVD010000000
VERSION     DAJSVD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.084"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(116..673)
                     /gene="frr"
                     /locus_tag="ON498_002048"
     CDS             complement(116..673)
                     /gene="frr"
                     /locus_tag="ON498_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3940291.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(676..1398)
                     /locus_tag="ON498_002049"
     CDS             complement(676..1398)
                     /locus_tag="ON498_002049"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3940292.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1534..2415)
                     /locus_tag="ON498_002050"
     CDS             complement(1534..2415)
                     /locus_tag="ON498_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3940293.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2514..3296)
                     /gene="rpsB"
                     /locus_tag="ON498_002051"
     CDS             complement(2514..3296)
                     /gene="rpsB"
                     /locus_tag="ON498_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3940294.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3655..4134
                     /locus_tag="ON498_002052"
     CDS             3655..4134
                     /locus_tag="ON498_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR3940295.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4351..5442
                     /gene="phnW"
                     /locus_tag="ON498_002053"
     CDS             4351..5442
                     /gene="phnW"
                     /locus_tag="ON498_002053"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR3940296.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5435..5563
                     /locus_tag="ON498_002054"
     CDS             5435..5563
                     /locus_tag="ON498_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3940297.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5567..6112
                     /locus_tag="ON498_002055"
     CDS             5567..6112
                     /locus_tag="ON498_002055"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3940298.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6566..8257)
                     /locus_tag="ON498_002056"
     CDS             complement(6566..8257)
                     /locus_tag="ON498_002056"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3940299.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8677..9624)
                     /gene="arcC"
                     /locus_tag="ON498_002057"
     CDS             complement(8677..9624)
                     /gene="arcC"
                     /locus_tag="ON498_002057"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3940300.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9739..10758)
                     /gene="argF"
                     /locus_tag="ON498_002058"
     CDS             complement(9739..10758)
                     /gene="argF"
                     /locus_tag="ON498_002058"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3940301.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10849..12078)
                     /gene="arcA"
                     /locus_tag="ON498_002059"
     CDS             complement(10849..12078)
                     /gene="arcA"
                     /locus_tag="ON498_002059"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR3940302.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3391 a         2536 c         2208 g         4184 t
ORIGIN      
        1 ttgttttttt atttaaaaac tgaataattt tttctttttt taatttttaa ataaaagaaa
       61 tataaataaa aacagaggaa gcggaatgat ccgcccctct gtttttatga ttttattaaa
      121 cttccaataa ttctttttct ttatcagcag cgatcttatc tacttctttg atgctgttat
      181 ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc atcttcggta atatcaccat
      241 ttttttgttg ttttttatat tcatcgatcg catcacgacg gatgttacga atcgcgattt
      301 ttgcattttc tgcttctttt ttcacatctt tagctaattc ttttctgcgt tcttctgtca
      361 gttgcgggat aactaagcga atgacatttc catcattggt tggactgatt ccgatatcac
      421 ttgccataat agctttttca acatcttgaa gaatgctctt gtcaaaaggt gtgatcatta
      481 aaacacgtgc ttctggaatt tggattgatg ccatttggtt caatggtgtt ggcgcaccgt
      541 agtagttcac agtaatacgg tccaaaaggc tagcgttcgc acggcctgcg cggatttgcc
      601 caagttcacg ttgaagattt tgtgctgctt ttttcatttt ttcttttgct tctgtcataa
      661 ttgcatctgc catttttatt tcccccttac agttgttccg atattttcgc ctaagattgc
      721 acggcgaata tttccgtgtt catttaaatt gaagacaagc agtggaatat cattatccat
      781 gcttagtgaa cttgcagttg aatccatgac ttgtaatcct ttggcaataa cttccagatg
      841 agtcagttct tcaaatttta ctgcatttgc atctactttc ggatcagcag aataaacacc
      901 gtctacattg tttttagcca tcaggataac atctgcgccg atttctgccg cgcgtaatgc
      961 tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg cctgcaaaga taacgatccg
     1021 tcctttttct aaatgacgtt ctgctttacg gcgaatatat ggctctgcga tttgacgcat
     1081 ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt tccaacgtat cctgtaaagc
     1141 taaggcattc ataactgtag ctaacatacc catgtaatca gcttgagcac gttccatgcc
     1201 catttgtgct ccgatttgtc cgcgccagat atttccgccg cccacaacga ttgccatttc
     1261 aacgcccagc tcgtgaactt ctttgatttc ttggatgatt tctttgatta ctggtggttt
     1321 gattccaaat cctgcgtccc cagctaaagc ttctccactc aattttaaga ctacacgctg
     1381 atatttcggt gtgaccatgt tcattcctcc aatattttct attgccattt taccatactt
     1441 agatgatttc gacactctaa gactggtttt tcttgttttg cttaaaaaaa gggagcgcac
     1501 aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt ttcacttggc tcataacttc
     1561 ttctacaaag ttatcttcgc gtttttcgat accttcgcca acttcgaaac gtacgaatgt
     1621 tttaacagtt gcgccttttg atgcaacgta tttttcaaca gtcatatcag gatctttaac
     1681 gaatggttgg tccaccaaag cgatttctgc tttgaatttc ttcaagcgtc cttctaccat
     1741 tttttcaacg atattcgctg gtttgccttc gttcaatgct tgttctgtaa gaacagtttt
     1801 ttcatgttct aattcagctt ctggaatttg tgattcgttt acataacgag gattgatagc
     1861 tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca gttgttccat ctagaacagt
     1921 caatacggca atacgtccgc ccatatgcaa gtatccacca aatgcagcat tgtcgtcttt
     1981 ttctacaact tcaaagcgac ggaagctgat tttttctccg ataacttgag ttgcttcgat
     2041 caagtcagat tcaattgtac ctttatctgt tttgattttc attgcagctt ccatgtctgc
     2101 tggtttgttt tcagcaacaa gttctgcaat ttcttttact aaatcttgga acatttcgtt
     2161 tttagaaaca aagtctgttt ctgagtttac ttcaacgatt gctgcagtgt tacctttcac
     2221 agcaactgaa gccaatcctt cagctgcaat gcggtcattt ttcttcgcag cctttgccat
     2281 tcctttttca cgtaaaagat caactgcttt ttccatgtcg ccttctactt caactaatgc
     2341 ttttttcgca tccatcattc ctacgccagt catttcgcgt agttctttta ccattttagc
     2401 tgtaacgtct gccatgttta actcctcctg tgtgtttttc tttttaaaaa aagctgcttc
     2461 aaaggagagt gaggcttgtc gcccagcctt tgaaacagct ccatcattca aaattattct
     2521 gctgaagaat tgtcgccttc aacaacatct acgatttctt cgattgatgt tgctgtgttt
     2581 tcttctgtga aatcttcttc aactacttga tcttcacctt ggtttccttc gatgaaagca
     2641 tcagccattt ttgaagtaat caatttaacg gcacgaattg catcgtcgtt tgaagggatt
     2701 actacgtcga tttcatctgg atcgcagttt gtatcaacca tagctacgat tgggatattt
     2761 aatttgtgtg cttcttgaac agcaatacgt tctttacgtg ggtcaacgat gtacattaca
     2821 tctgggattc ttggcatatc agcgataccg cctaagaatt tttcaagacg ttcacgttct
     2881 ttgttcaaac cagcaacttc ttttttaggt agaacttcaa aagttccatc ttcttccatt
     2941 gcattgattt gtttcaaacg gctgatacgt ttttggatag tatcccagtt tgtcaatgtt
     3001 ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac gagttgcttc ttctttgatt
     3061 gcttcttgtg cttgtttttt tgtacctacg aataatgcta cgccgccttc ttcagcaacg
     3121 ctcttcatgt aatcataagc tgcatctact aatttaactg ttttttgcaa gtcaatgatg
     3181 tagattccgt ttctttctgt gaagatatat ttcttcattt ttggattcca gcgacgagtt
     3241 tggtgaccaa agtgtacgcc ggcttctagt aattgtttca tagaaattac tgccatgttt
     3301 gtttcctccg atttggtttt atttttcctc ttcttatctt ctaccttgcc ggcaaacttt
     3361 ttcaagcacc taccgacaat ccaataagaa tgtggatttg ctgatgcata aagcaccctc
     3421 agctattata cagacaaaag atccgtatcg caagagcaaa tatacttttc tgctcaacat
     3481 tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa cagtagattt ctctttgttt
     3541 ctttattatc ctttttccat aaaataattt taggtataaa agacgaatat tattgggcaa
     3601 tccttctttt ttttgttatg atacatgtga aatctttcgt aaaggagaaa gaaaatgcgc
     3661 aaagctcaaa gacatttgct gattaaacaa gtcattgaag aattcccgat ccgtacgcaa
     3721 gaagaactat taaacaaact tgggcattat ggcgttaccg ccacgcaggc aacgatttct
     3781 cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt ttcccgcttc
     3841 gtattgtttc gcgaaaaagg aactcctgag actaggactg aggaagagga tcggctggtt
     3901 caaatgattg aagacattgt attgaaagtc gaacgagtcc actttttgac gatcgtcaat
     3961 accttgccag acaatgctca tttatttgca tctgttttag atgagatcaa acctccctct
     4021 attgtcagta caattgctgg attcgatacg atcatggtca tctcggaaaa tgaggaggac
     4081 gcaaaacgag tagaagaatt tcttcaccat ccaactgaag tatcactttt ttaatcagag
     4141 cacgtattcc taatatggaa tgcgtgtttt tttattttca atattttcct tattgaataa
     4201 acctttacac tctttacaaa aatacatcat tttttattca aatttttcaa caccttgatt
     4261 gaatcacaat aatcatcatt tatattggat gttaacaaga gcagcttctt gctctttgtg
     4321 tatttcaaaa aagaaatgaa aggaaatcat atgtataaat tattaacttc tggaccattg
     4381 acgacttctg aaacagtgaa aaatgctatg ctgatcgatc attgtacttg ggatcaagaa
     4441 tacaaagaaa ttacacaatg gataagaaaa aaattattgg atcttgctca agtatcggaa
     4501 ggaatataca caacggtctt aatgcaaggt agcagctcct tcggggtaga atcggtatta
     4561 agttctgtga tcaggccaga agatactgta ctgatttgtt caaacggcgc atatagacaa
     4621 cgaatgatca acatgtgcga atgtctgaaa ctgaactata ccgtttatga ggtatcggaa
     4681 catcaaatcc tccaagctca tgtgattaca cagctgattg aagagaatcc gtctatctcc
     4741 gcactagcaa tggtccatag cgagactact tccggtattt tgaataattt ggaagagatt
     4801 gctgctgtca cacaagaaag agatcttttg tttatagtgg acgcgatgtc aagctttggt
     4861 gggataccga tttgtgtagc cgaactaggg attgattttt tgatttccag ctcaaacaaa
     4921 tgtattcaag gtgttccagg cttttctttt gtacttgcta ataaagcgga actggaaaaa
     4981 cgaaaaaatg ttagccgaag tttgtcactt gatctatatg accagtaccg cgtgatggaa
     5041 aacgagaatg gcaaatggcg tttcacttcc cctacccata cggttttggc ttttcaacaa
     5101 gcgctgaagg aactagaggc agaaggagga atcgaaactc gatattcacg ttatctcgcc
     5161 aataatcatg tgctacgaca aaaaatgaca gagatggggt tccagccttt cattcaagaa
     5221 tatcaggggc cattcatcac ctcttttctt taccctaagg actggcgatt tgattttaat
     5281 catttttatc agtatttaaa gcaaaatgga tttgtgatct atcccgggaa aatcagcgat
     5341 catgaatgtt ttcgtatcgg aaatattggc gagatctatc cggaagatat tttggaactg
     5401 acagcaatta tcaaagacta taaggagaac gttaatgatt aaaactgtaa tttttgattg
     5461 ggcaggaaca actgtggact ttggctgtat ggctcctgtc cacgctttta gaaatgcttt
     5521 tctagaaaaa ggaacccaac tgactgacaa agaaatccgc taaccgatgg gaaaattaaa
     5581 atgggatcat atccaacagc ttctttccct cccttctgtc aaaaaacaat ggatagctat
     5641 ttatggacaa ctacctcagg aaaaagaggt ggaggaattg tatcaacaat ttgaacatta
     5701 tctgtttaaa gatttggtca agcattctac cttgaaaccc gacacattgg aaacgatcga
     5761 acggttgaga aacgctggga tcaatattgg ttccacgaat ggatttaatg ctcctatgat
     5821 gacaatcgta gcagaaaccg caaaaaaaga aaggtattca ccagacttca tggcaacttt
     5881 tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg atccataaaa acatgcagta
     5941 cttcaaaaac aagtcggttg atgaagtaat caaagtcgga gatacggtct cagatatcca
     6001 ggaagggaaa aatgccggag tgttgacagt aggtgtaatt gaaggtagtt cgctaatggg
     6061 gatttcttat gatgagtacc aaaaattaga gatggggaaa aagatcgttt aaaacagtct
     6121 gttgctctta gattccaaca agccggcgct gatatttgtg taaacaattt aaaagagtta
     6181 acagaataca ttttgtacgc tgacaagaat ccagaatgac ttttatacaa aataagaggt
     6241 tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca agcaaaaata gctcctcata
     6301 tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
     6361 aaaaaaaagg gagctgtgac acgacttttg tcacagtccc cttatttgca aacaaacagt
     6421 gtttaagaga caacctctcc actaaacggt gttcattcag ctgactctcg gtatttgctc
     6481 aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt tctctaatac tcggatcaga
     6541 acgctgattt cacattctct ttttcttaca tcttatctgg tgcatgaagt cctagcaaac
     6601 gaaggtcttc tttcaataat acagtcactg cataaactag agctaagcgt gcttcttttt
     6661 gttcatcatc agctaaaatc ttcgtatgag catagtattt gttgaatgct tgtgctaatt
     6721 tgattgcatg tttcgcaatc actgacggtt cgtatttttc cccagcagac aagaccgttt
     6781 cagggtattt ttgaactaat ttaaccactt cccagctggt atcgtcattc aatgcatagt
     6841 ctgctgtttc tgaagggaca aatcctgctt tttccaacag gctgactgca cgagcatgtg
     6901 tatattgcac ataaggtcca gtttctcctt caaatcgaac aacttcttcc aggttaaagt
     6961 caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat gactgcccca accccaactt
     7021 gtttagctac agcatctttg ttttccaaat cagggttttt ctcgctgatt tgttctttag
     7081 cagattggat tgcttcattt aatacttctt ctaataagac gatctttcct ttacgagttg
     7141 ataatttttt accaccttgc gtgatcaaac caaatggaat atggtgcatg tcgtcagacc
     7201 agtcaaagcc catttccttc aagacagctt tcaactgttt aaaatgatag ctttgttcat
     7261 tccctactac atatagagat tgtttgaagt cgtaagtacg tttacggtac aatgcagcag
     7321 ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac gctggattca
     7381 ggtcataagc agataggtca acgatctctg cacctttatc ctcgttcagc aaatgttttt
     7441 cttctaaaag tttgacgatt tcatccatct tgtcattgta gaaagcttca ccgttcaacg
     7501 aatcgaaacg tacttccagc agatcataga ttttattgaa ttctttcatc gattcatcac
     7561 ggaaccattg ccataattga atagcctctt tatcgccttc ttccagacgt ttgaaccaag
     7621 cacgagcttc ttcatttaat tctggttctg tttcagctac ttcatggaat tggacgtata
     7681 aacgaagcaa ttcattgatt ggttctgctt taacagcttc ttctgtaccc cattttttgt
     7741 aagcgacgat cagttttcca aactgtgttc cccagtcacc taaatggttg atgcggattg
     7801 gctgataacc gattttttcc ataatgaaac cgatcgaatt accgataact gtcgaacgca
     7861 aatgtcccat tgagattggt ttggcaatat taggagaaga catgtcgatt ggtactgttc
     7921 cttgattccc gatgttgcta tccccataat gttctttttc tttcactact gtttgcaaaa
     7981 cttttttgct gataagttct ttattcatga aaaagttcaa gtaaggtccg acaacttcga
     8041 ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt tgctgtggcg
     8101 cttttcggta aacttttgct aatgaaaagg ctggaaatgc aacatctccg tgttcagctg
     8161 attttggatt ttctagtaat tgctcgactt gttccagtgt taaatcatct ttgacagcgt
     8221 catagacagc ttttgcgaca agatctttat tattcatctt tattcctcct aaagtgttcc
     8281 cttgattgtt aaacaaaaaa aatctctagc gacggcatac ttagtatgcc atcactagag
     8341 acgagatttc ccgcggtacc actccaattg tcctaaaaaa ggaccagctc catctgatat
     8401 cggtagacga ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg tatcttcttc
     8461 attcggcttc caccacttcc gaattcgcta gccaagaatg atacaagtac tctcttgttc
     8521 aacaaatctt agtcttatta aactttcttg aattatatca gaaaaaaaaa cgaaaggcta
     8581 gtcgtttcat aaaaaaaaaa tccagatggc ctaaaggaga agaaatacca cctggatagc
     8641 tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga taatcgttcc
     8701 ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt
     8761 tggttttgct tcaacaaatg caatagctgc ttctactttc ggaagcatgc ttcctggagc
     8821 gaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt ctaatttctt
     8881 ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata cgatcagcaa
     8941 atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga ttacggcttc
     9001 tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac caacagagac
     9061 tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa tcgagatggg
     9121 tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga atgtcacagt
     9181 ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac cgactggttt
     9241 acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga ctaaagaaac
     9301 cacatcttta tcgatccctt tttccttcag tacttcgccc atggcgtttt gtaaccagta
     9361 accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc
     9421 agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat ttccgtggga
     9481 gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg tttccattaa
     9541 cgcttcttgt tgggcttttg cacttgcatc cgtagacaag atggcattgc cccctaaagc
     9601 gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac cgaattttct
     9661 agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg ttttttcgtg
     9721 agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg ttgctgccat
     9781 gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag catatttgct
     9841 gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt tcatttgttc
     9901 accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga tcaaacgatc
     9961 tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca acaatttgat
    10021 acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat aaaggacgtc
    10081 tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac cggatttttc
    10141 agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac
    10201 gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag ccatattgtt
    10261 tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt tttcttggat
    10321 agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc cattccaaac
    10381 tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc cacggaattc
    10441 gattccgtca aacatacgtc ctagtacttt agctgtatct tctgtggatt cttttttacc
    10501 taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga tcgctgcagt
    10561 agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc
    10621 ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc
    10681 gatcaaatat tgtaattctt cttttgtaaa gtctttttct gctaataagc ttcttccttg
    10741 gaatactgat tctttcatat taaacattct cctttagatt gtttttattt aagattaaag
    10801 aggaaatccc gacggcagct gacagtaaga ctgccgtcag gaaacaaatt acttcaagtc
    10861 ttcacggaca agtggttggc tcatgcagcg tggaccacca cgacctcgtg acaattcact
    10921 tgaattgatt tctaacactt tgatgccata gctacgaagc aattcgttcg atacataatt
    10981 tcggttataa gtgactacaa cacctggagc gattgccaat gtgtttgatc catcattcca
    11041 ttgttctcga ggtgcaacga tctcatctcc attaccagtt gggatcagta caagatcatc
    11101 taatccaaga gcagctttca tcgtttcgtg taagttatct gaatgagtaa tcttgatatc
    11161 atcaccatct ggttcgattg taaagacatc catcttaccg tctttacttt gaattgctgg
    11221 atggatagtg aatttatcgt gatctaccat tgtaaatacg gtatccaaat gcatcattgc
    11281 acggttgttc ggaattttga ttgccaacac tttttcaaat cctgaatttt ttgcgaatag
    11341 gtggcgtgct aatttttcta aagctttcgc attagttcgt tgagaaatcc caacagctac
    11401 tactttatcg cttaagatca attcgtctcc gccttcgata tgatctggat tttcacgatc
    11461 caaccatact tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa tatattctgt
    11521 aaacatcgat tcacgacgac gtgcttcaaa agtcatctta ttgactgtca tcccatttcc
    11581 cattgatgcg gaagggtcgc gtgtaaagta caagtttggc attgggtcca tgtaaaatgg
    11641 atagtcatcg tctgctgaca gatcatatag actgctagaa cgtacttcga tatcttttgt
    11701 acgaacccca gccatgattt tatctaccat ttcttgcgtt tccattgata gaaggaactc
    11761 atgaagaccc tctcttacag cattcgatgc aatatgagat tcatccaaca ttttattcaa
    11821 gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag
    11881 agtctcgacg ccttcatttt gcaatgtctt cgcaaagttg tcatgttctt cttgagcaat
    11941 cggaagatat ggaatatcat cgaacaacag acgatccata atatctggtg tcaaattttc
    12001 cacttcttgt ccaggccgtt tcagtaaaac tgtctttaat ttccctattt cagagaaaac
    12061 gtgaataggt ttatccatgt tgtgttgcct ccttcttttt gattacatgt ataggatatc
    12121 ctccttttgt aatcactttc ataattttgt ctcattcaaa atgagacatt cctcacatta
    12181 atattcgata attaaataat taaacgtttt tctattttta agcgtaattt attcattttt
    12241 tattctgtat acactttcaa aaaaatattt attcattgaa aaaatttttt tgaataaatt
    12301 aaaaaaataa attatatta
//
LOCUS       DAJSVD010000083        12205 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSVD010000083 DAJSVD010000000
VERSION     DAJSVD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.085"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(102..290)
                     /locus_tag="ON498_002060"
     CDS             complement(102..290)
                     /locus_tag="ON498_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3940303.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            560..922
                     /locus_tag="ON498_002061"
     CDS             560..922
                     /locus_tag="ON498_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3940304.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            974..2656
                     /locus_tag="ON498_002062"
     CDS             974..2656
                     /locus_tag="ON498_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR3940305.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2773..4809
                     /gene="recG"
                     /locus_tag="ON498_002063"
     CDS             2773..4809
                     /gene="recG"
                     /locus_tag="ON498_002063"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3940306.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4858..5859
                     /gene="plsX"
                     /locus_tag="ON498_002064"
     CDS             4858..5859
                     /gene="plsX"
                     /locus_tag="ON498_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3940307.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5981..6223
                     /gene="acpP"
                     /locus_tag="ON498_002065"
     CDS             5981..6223
                     /gene="acpP"
                     /locus_tag="ON498_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3940308.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6491..7495
                     /locus_tag="ON498_002066"
     CDS             6491..7495
                     /locus_tag="ON498_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940309.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7496..8440
                     /locus_tag="ON498_002067"
     CDS             7496..8440
                     /locus_tag="ON498_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940310.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8440..9402
                     /locus_tag="ON498_002068"
     CDS             8440..9402
                     /locus_tag="ON498_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940311.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9429..10343
                     /locus_tag="ON498_002069"
     CDS             9429..10343
                     /locus_tag="ON498_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940312.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10369..12150
                     /locus_tag="ON498_002070"
     CDS             10369..12150
                     /locus_tag="ON498_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3940313.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT         4014 a         2184 c         2714 g         3293 t
ORIGIN      
        1 aaataaacgc aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt tttattaaat
       61 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
      121 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      181 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      241 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      301 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      361 tagaatggga atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      421 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      481 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      541 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      601 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      661 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      721 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      781 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      841 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      901 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      961 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
     1021 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1081 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1141 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1201 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1261 taagcaaatt ccagatgttg tctcattaaa tgcttctgat cttgctgcag ccttcactca
     1321 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1381 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1441 agtaatgaca gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc cagacttgct
     1501 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1561 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1621 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1681 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1741 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1801 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1861 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1921 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1981 gaaaaaagaa ataccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     2041 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtggac aaacaatgaa
     2101 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2161 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2221 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2281 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2341 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2401 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2461 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2521 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2581 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2641 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2701 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg
     2761 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2821 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2881 tatccttttc gctacgaaga tattcaggaa agaaatctaa acgagatcca ggatcaagaa
     2941 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     3001 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3061 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3121 tgggatgcaa agcgtaaagc cttgaacggg atgaagattt taggttctca aacaatggat
     3181 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3241 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3301 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3361 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3421 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3481 ctttatgatg tccaaaaatt gaaacaattt actcaaaagc taccttttga actgacaaat
     3541 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3601 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3661 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3721 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgatt
     3781 acggggtcaa cgaaagcaaa agaacgacgt gagctccttg aaaaactaga gcaaggggaa
     3841 attaacgtcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3901 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3961 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     4021 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4081 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4141 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4201 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4261 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4321 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4381 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4441 gcacagctgc accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4501 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4561 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4621 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4681 gttttggagg tagccagaga agaggctgca gccctttggc aaataaagga ttggcagatg
     4741 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4801 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4861 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4921 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4981 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     5041 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5101 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5161 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5221 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5281 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5341 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5401 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5461 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5521 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5581 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5641 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5701 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5761 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5821 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcgat
     5881 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5941 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     6001 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6061 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6121 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6181 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6241 gaaggataaa aagctgcatg cagcttttta tccttctttt tttaatcttt ttgacacgta
     6301 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcggt
     6361 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6421 gatttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6481 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6541 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6601 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6661 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6721 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6781 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6841 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6901 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6961 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     7021 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7081 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7141 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7201 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7261 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7321 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7381 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7441 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7501 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7561 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7621 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7681 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7741 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7801 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7861 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7921 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7981 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     8041 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8101 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8161 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8221 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8281 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8341 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8401 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8461 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8521 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8581 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8641 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8701 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8761 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8821 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8881 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8941 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     9001 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9061 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9121 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9181 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9241 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9301 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9361 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9421 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9481 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9541 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9601 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9661 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9721 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9781 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9841 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9901 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9961 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
    10021 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10081 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10141 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10201 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10261 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10321 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10381 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10441 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10501 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10561 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10621 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10681 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10741 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10801 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10861 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10921 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10981 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    11041 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11101 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11161 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11221 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11281 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11341 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11401 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11461 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11521 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11581 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11641 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11701 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11761 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11821 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11881 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11941 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    12001 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12061 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12121 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12181 cctttttcgg ttttttgata ttttt
//
LOCUS       DAJSVD010000084        12164 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSVD010000084 DAJSVD010000000
VERSION     DAJSVD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.086"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="ON498_002071"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="ON498_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="ON498_002072"
     CDS             complement(1437..2279)
                     /locus_tag="ON498_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3940314.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="ON498_002073"
     CDS             2604..3020
                     /locus_tag="ON498_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3940315.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ON498_002074"
     CDS             3017..3487
                     /locus_tag="ON498_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940316.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ON498_002075"
     CDS             3501..4274
                     /locus_tag="ON498_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940317.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ON498_002076"
     CDS             4288..5100
                     /locus_tag="ON498_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3940318.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ON498_002077"
     CDS             5154..6119
                     /locus_tag="ON498_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3940319.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="ON498_002078"
     CDS             6256..8979
                     /locus_tag="ON498_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3940320.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="ON498_002079"
     CDS             8992..9624
                     /locus_tag="ON498_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3940321.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="ON498_002080"
     CDS             9829..10056
                     /locus_tag="ON498_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR3940322.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="ON498_002081"
     CDS             10125..10391
                     /locus_tag="ON498_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3940323.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="ON498_002082"
     CDS             10381..10650
                     /locus_tag="ON498_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3940324.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="ON498_002083"
     CDS             complement(10829..12130)
                     /locus_tag="ON498_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3940325.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3972 a         1973 c         2387 g         3832 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgtggtg attc
//
LOCUS       DAJSVD010000085        11863 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSVD010000085 DAJSVD010000000
VERSION     DAJSVD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.087"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ON498_002084"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="ON498_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3940326.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ON498_002085"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="ON498_002085"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3940327.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="ON498_002086"
     CDS             complement(2169..2906)
                     /locus_tag="ON498_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3940328.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="ON498_002087"
     CDS             complement(2997..3524)
                     /locus_tag="ON498_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940329.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="ON498_002088"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="ON498_002088"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3940330.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="ON498_002089"
     CDS             complement(4401..4886)
                     /locus_tag="ON498_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3940331.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="ON498_002090"
     CDS             complement(5039..5236)
                     /locus_tag="ON498_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940332.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ON498_002091"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="ON498_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR3940333.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ON498_002092"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="ON498_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR3940334.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="ON498_002093"
     CDS             9211..9423
                     /locus_tag="ON498_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR3940335.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="ON498_002094"
     CDS             9425..11104
                     /locus_tag="ON498_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3940336.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="ON498_002095"
     CDS             complement(11358..11558)
                     /locus_tag="ON498_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3940337.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..>11863)
                     /locus_tag="ON498_002096"
     CDS             complement(11708..>11863)
                     /locus_tag="ON498_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HCR3940338.1"
                     /translation="SGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLRE
                     EGMMEEK"
BASE COUNT         3431 a         2567 c         2064 g         3801 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcaacggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc aga
//
LOCUS       DAJSVD010000086        11571 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSVD010000086 DAJSVD010000000
VERSION     DAJSVD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.088"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..366)
                     /locus_tag="ON498_002097"
     CDS             complement(<1..366)
                     /locus_tag="ON498_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940339.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTR"
     gene            complement(666..1547)
                     /gene="galU"
                     /locus_tag="ON498_002098"
     CDS             complement(666..1547)
                     /gene="galU"
                     /locus_tag="ON498_002098"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3940340.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1590..2609)
                     /locus_tag="ON498_002099"
     CDS             complement(1590..2609)
                     /locus_tag="ON498_002099"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3940341.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3242..4246)
                     /locus_tag="ON498_002100"
     CDS             complement(3242..4246)
                     /locus_tag="ON498_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3940342.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4259..5308)
                     /locus_tag="ON498_002101"
     CDS             complement(4259..5308)
                     /locus_tag="ON498_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3940343.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5325..6146)
                     /locus_tag="ON498_002102"
     CDS             complement(5325..6146)
                     /locus_tag="ON498_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3940344.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6133..6912)
                     /locus_tag="ON498_002103"
     CDS             complement(6133..6912)
                     /locus_tag="ON498_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940345.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6929..7399)
                     /locus_tag="ON498_002104"
     CDS             complement(6929..7399)
                     /locus_tag="ON498_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940346.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7411..7830)
                     /locus_tag="ON498_002105"
     CDS             complement(7411..7830)
                     /locus_tag="ON498_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR3940347.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7911..10688)
                     /locus_tag="ON498_002106"
     CDS             complement(7911..10688)
                     /locus_tag="ON498_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3940348.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            10879..11103
                     /locus_tag="ON498_002107"
     CDS             10879..11103
                     /locus_tag="ON498_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940349.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3336 a         2446 c         1889 g         3900 t
ORIGIN      
        1 tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc
       61 ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta gatacagtac
      121 agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa
      181 ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct
      241 gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt
      301 gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt
      361 tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt attctactat
      421 gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa
      481 aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc
      541 tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa
      601 tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt
      661 tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa
      721 gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta
      781 tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta
      841 agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct
      901 aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa attacttggt
      961 gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta
     1021 tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg
     1081 gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata
     1141 tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct
     1201 tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga
     1261 ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa
     1321 ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc
     1381 gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc
     1441 gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa
     1501 cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcacttg ctgttcctcc
     1561 taaacgacga ttaactattg atcatctatt taacgaagag aaaattcatt ctccattttt
     1621 ccgtcacgta acatgatttc ttttgctgct tttttgatat cttctccatt atacaaaaca
     1681 ttatagattg tttgagtaat tggcatctca acacctagct gttgagctag ctctactgct
     1741 gcttttgtcg ttgccacacc ctcaacgacc attcccatgt tttctagaac ttcttccagc
     1801 ttatgtccct gtcctaacaa gttaccggca cgccaattgc gagaatgaac acttgtacaa
     1861 gtaacgatca agtcccccac accactcaaa ccgataaatg tcagaggatt ggcacccatc
     1921 gcgacaccaa gacggctgat ttctgccaat cctcgagtca tgattgctgc ttttgcatca
     1981 tctccgaatc ctaatccatg gatagcgccg gcaccaattg caatgatatt tttcagtgct
     2041 gcaccagttt ctacgccaat cacatcatta tttgtataaa tcctgaaata atcattcatg
     2101 aacagttttt gtacataggc tgctgctttt tgatcaatac ttgcagctgt aattgttgtg
     2161 atatcatgta ctgccacttc ctcagcatgg ctaggaccag ataatacaac aattgcctgt
     2221 cttttttctt ctggaatttc ttcagccaaa acttctgaga ttcgtttatg cgtatcttgt
     2281 tctagtcctt tacttgcatg tataatgatt ggcttagtat tcattttacc aataagttcc
     2341 tgtgcgactg aacgaatcgc ttttgttgga acaacaaata aaatagcatc tgcgttatcc
     2401 actgcttctg ccaattcttt atatccgata atcgattctg ggatcgtcaa atcaggtaaa
     2461 aaatgctgat ttgtatgata cttattgatt tcatcaatct gttcaggtac atttccccaa
     2521 atacggacat catgcccgtt ttctgccaat gtttgtgcta aagcagtgcc ccaagatcca
     2581 ggaccaagaa ctgcgatttt ttgtttcatg gatattcctc caaataaata attgataatt
     2641 tccattatag tatacatttc ttaaagttgg gactaatttc cattgttttt aagtgtttgt
     2701 ttacttcttt tatcgttttt acttgaaata atcaatgata tgttacaata gtctacattt
     2761 tatttttaca ctcgttatgc tttgcttgtt cctaagagga agcagttttg acaatgttac
     2821 taccagcatt actattagga ggttaggaaa cttctcctag tgagtattga tttaaaatac
     2881 ataagagaca gattgaacat ttctaaatct gtcaggtcct gcaacggaca gattgtctct
     2941 gttgacggtt gacataggaa cttttgtttt agtcaacatt ctggatacaa aacgctatgt
     3001 aaactggcgc aactcgttgt acttggactt ttactacgga tccatgcaac aactaaatag
     3061 tactattgca taacaaaaaa tggttggcac ccacatgtcc cattaaacag attataagtt
     3121 gaactgtaga taatgcaaaa aatgtgtttt ataacgtaat tgatgccaat attttaaaaa
     3181 aataaaggga gcagttatcg ctgctctctt tattttttat attcttttta tccagtctat
     3241 cctaataatc aaactgacga tagtagcggc gtatctctaa tggatggcag ttcaactgct
     3301 ccacatgagc atcgatgcgt tgtgtcacta catgcatcaa taagaaagac aaaacacgtc
     3361 cgcgatattt ttcactgata ccttctactg gatagtcttt tgtgtctatc aatgtataat
     3421 tgctgcaaat ttttggcaaa agtttagcca cacgttcaga taacactctc tgttcatctt
     3481 cgcctaaaaa taatgtgact ggggttgttt cttctacgat ctctaacgtc ccgtgaagaa
     3541 actcggcagc atggatagat ttcgatggaa gccagctttg ttcttcccag taacacatgg
     3601 catatgaata aacagctccc cattgattcc ctgctccaat aaaataatgc atactatcac
     3661 gtcgatgttt ttctgcaaat gataagccaa atgcatctgc ttttttctct gcttccacta
     3721 accctgttgg taaataacgt tcgagctgtt catagtattc tgaataagct tcgaattctc
     3781 cattcaaata catcaatcga tctgctgcca taaaaaattt gatttgttct gttccaggag
     3841 ctggataagt gaccacaaaa tcgcatagct gagacaactt actgttcgct ttatcgataa
     3901 atccaatcag tgtcgctcct actttcttga tttgttctac tgctttcact acttcttgag
     3961 ttgtaccggt aacagaggac aaaataacga tagaatcttt tgttaatctt ttgtttccag
     4021 tagtgtaata ttctgcagca tgctgtacaa agacaggcaa gttgcttttc ccattcatat
     4081 aagtgacagc ttgcatcgag gaagcatacg ttccaccgat tcctagataa tagacagcgt
     4141 caaatttttc cgcataaagt ttatcgatga tttcttctac ttgcggacgt aaagctagtg
     4201 cgccttttat tgctttgatt tgttcttctt cattgaattt cagcatgatt tccacctctt
     4261 ataatttact tttcaagact gtatcaaagt cttcaaaaat ctttttgttc aagtcaatac
     4321 ctttagctac tgcggtttta taagcgaata gttgaaacgg aacgacaagc gctaatacag
     4381 acagatattc atcacattct attgctaatc caagtgtttt ctcgtttctt gctttttttg
     4441 tggtaatggt gagagtctct ccaacgtacc gactcatata tctttgcaac ttttgcgaac
     4501 gttccttatt tacactttcc ccttcaataa agaatagcaa atgcgaagca tctgcctcca
     4561 ggtatgggcc gtgcatataa gcttctaact caaatccttg tgacggaacg cggattgttt
     4621 cagtcaattt cgtctctgct tctttagctg ttccccagtt tggaccatat ccaatggtga
     4681 cgaaacgctg tgctaaacga aataaacttt gatatccatc aaaaaattct tccgtttttt
     4741 gaataatcgc tggtaaatgg ttgattattt tgcgcaactg ttgcatgtag tcttgttcta
     4801 tttttttgct gattttattt ttacttattc caattcctat ccctaagagt agtaactgta
     4861 aaacagtcgc agaatagcct tttgtaacga agcctactga ttctaccccg gcacctaaat
     4921 caataatttg tgcagcttct aaagcaagtg gactttgcac gtcatttgtg atagcaacaa
     4981 caggtaaccc ttgtttttgt atcattttta ctgcttcaac agtagaagca cttttcccgc
     5041 tttgtgacac agcaatcaca gtatcgattg aaggagaaag ttttccataa ttgaaaaaat
     5101 aaaaaggttc ttgaatatcg acagtaagat caccataaga ttccaatgct ggcttcgctg
     5161 ctaaacaagc gttataagat gaacccgttg ctaagatcag taaattcact gtttttttca
     5221 tatgttctct tgtcgaaaaa tcattctttt tcaagatcgt acttagtact tccttttctt
     5281 catagatata atcttgcatc gtaaacattt tctctgctcc ttttttaaaa aatccctatc
     5341 cacgaaccaa aaattccaaa gaaagctagt ccgatcaaga tcgttaatgg tttcacttct
     5401 ttcttcaata gataaaacac tatgccaaat gctccaagac ataagatatt tggaacgata
     5461 tcatcaaaaa tactttgaac agtcacagca ttttctcctg aaccgatttt taaaggaata
     5521 ttgatggtga tcatcgtggc agtcattgct ccaacgacca tcagtccaat aatagacgcg
     5581 cctaatgtca aactttccat cataccgttt gcctggatct ttttcaaaaa acccgttcct
     5641 aagcgatatc cccaacctgt tgctagatag cgaaacagaa tgtgcggaat attgaaaatc
     5701 aaaatgaaca agatagggcc taaaatattt ccttgcatgg ctaaagaggt acccacacct
     5761 gtggcaatca atcgcaaagt tccccaaaag aaagaatctc ctaaccctgc aagaggtccc
     5821 atcaaagatg ttttgatact atcgatcgca gtcacatcaa attcaggatc atccttattt
     5881 tcttcctcca ttgcagccgt gattcctaaa atcagtgtaa cgatatgtgg ggtcgtgtta
     5941 aaaaactcta gatgccgttt caaagctgct gccatttctt ctttcgactg atataacttt
     6001 ttcaaaatcg gaatcattgc ataaacataa gccatattca tctgacgttc ataattccaa
     6061 gaaaactcca tttgaaaaga acgccaaaat acgtgattta gctctttttt cgttattttg
     6121 ctgtttttga tctcagaatt cttcatcttc ttctacctcc cctgcagttg tttgaagtgg
     6181 tagttgacgg acattcataa tattgactac cacaactgct aaaatcgcac caaaaatagc
     6241 tactccggtc acaggaatct ctaagtaaga agctatagca aaacctaaga aaaaatatgg
     6301 tgcaacttgt ttgttgatca gcaaacgagc aagcatcgca aaaccaagag ctggaatcaa
     6361 ccctgtagct actgacaacc ccttttgaac gaagtcagga atcatattta atagatttcc
     6421 gatcgtattg cttcccacca tataagaaac cattaccaca acaccaagca tcaaggaaag
     6481 accgataccg gcaagcaagt gcatatgctc gactccttta taatttcctt cttctgcata
     6541 attgtctgct ttatggttca taattggaat caaaagccct aagtaaatat ttttcaatat
     6601 caatgtcaac gtagcaatcg gcaaacctag taaaagcgcg gtttctgtac cggctccagc
     6661 tgtaatagca aaagccgtcc ctaaaatccc accggtcaca acatcaggtg gaatcgaagc
     6721 acctactgag aacgatccga taaatgctaa ctctaaagtt gcgcccataa tgatcccagt
     6781 ttttacatca cctaacacga ttcctgtaaa cagccccgtc acaatcggac gagaaagcaa
     6841 cgatgtccct agtgcatatt ctgattgtgc gacaaatgca acaattccta ataagactgc
     6901 ttgtacaatc attgaggttc ttcctttctt acaaaacttc tcttacatcc acttttttat
     6961 ctgctggtac ttgacggatt tctacttcta tttgtttgtc aattaatcgt ttcaagcgtt
     7021 cctcctcttc tggtaaaata tttaccgctt ttccgatgct gcgagtacca ggttttgcct
     7081 tgatccctcc taagttgatg gttttgattt ctggatacgc ttctgccagc ttatatgcat
     7141 cctcgatcga ttccactacg atgaataact tatatttatc tgtcaccccg cttttcaacg
     7201 cggcaatcga gtcatcaatt gatttgatca ctagtttgac tccttgtggc ttggctaatt
     7261 tgatcgttgt ctttcgtatc tcattcgtag gtacatcatc attagcaatc aaaatacagt
     7321 ctgcgcctat tgtttgtgtc catgaaaaag ctacttgtcc gtgtaataac cgatggtcga
     7381 ctcttgtcaa tacaatcatt tttcttcctc ctaaaattcg tcttcttcta ttttctgatt
     7441 gagccgctcg ttacaaaact ggatcatttt ttttgaatca gttagcacct gctggatcaa
     7501 aacagttgtc tctacttcag cgtccaaccg agatactagc tcaatcaata gtggcaaatt
     7561 caatccggca acgagataga catgatcccg attgatatat tgtaagaatt cgttgttgac
     7621 acttccgcca aaaagatccg tcagaacaat gatttcttta tctttgtacc tcataaaaag
     7681 ctgccttact gtttccgcta aatcaaaatt ctcatcagta tagcaatcga atacagtaat
     7741 agaatgattt ttcccataga tcagctctaa tgaatctaat atccctgaag caaattttcc
     7801 atgggaagct aagataattt ctctatccac tttttcatct cctcttttca cttacacata
     7861 ctaaaaaagc aagttgcatg ccaaaattag tggcaacaac ttgcttatgt tcaaaattct
     7921 tcttctatcg agagaaactc tgtatcttca ctcaaaatat catagatata ggcaatctca
     7981 gaattgggga tcatgacact ataatgctct tcaatgacac taaacgcaga tttaatagca
     8041 gataaccctt ctttttgaca ctgcttccat ttctctaatc cttgataggt ttcgattggc
     8101 atttgtcgga tcaatcgttc aatgagacag ctcacatgga catacaacgc taattttttg
     8161 ggattgctga tgcttcgatt caactgcacc tctaattctc tcataaaatg atcgatttct
     8221 cccatcacct tctctgtatc tagaatagtc acggaatcca gtactttttc caatgaaaaa
     8281 ttacgaatga tgcgctgatt gaatatttcc ttctctgaag cattcatagt agtgtccaac
     8341 cattcagcta gtacagacat cttttccccc gagatgaggt cttctaatga aagatagtcg
     8401 atattggtga tatcaggatc tgtggtgcca atcattccta cgacttcgta tatggaaaat
     8461 aatgggtcac tgtttttctt atcttttaat tgctgatatt catatgggat gattttcaac
     8521 tgacaagccg taggtaagct tttttctagc agttcactta agtggaccgc cgtaccgatt
     8581 ccagtgaagc aagtagtaat gagtgctttg ggtttgtttt cctctggata gagcagtcgc
     8641 cattctggct ggatctgtcg aacagtctct tctgctatct cttctaatga tacatttttt
     8701 tgcaggcatt cccctactgt gattgctaaa ggtgtcgtca cattattcaa aatcaaaagc
     8761 ggtacggata actgtttttt aaaaaactga tggatttctt tcagagatcc catgtctact
     8821 aatatgatca atccatttga aacatcgctg ttttcgcaat aatcaaggat ttctgaagca
     8881 atctgctgtg gtgtgacatc tagtggcata tcaaatgatt caaatagatc ctcttgtaat
     8941 aaacgattta ctacatttgc catactgcta gcagttgcat aaccgtgagc agcaatcatc
     9001 gcttttggca gtcgtttttc ttttgaccat ttcgcttttt ttagatagat cgtcagtaaa
     9061 atccgatcca tacttgtgtc ttcaatatct aatttgatct tacttaattc cagaattcgt
     9121 tggacatagt gataactaga tggataagac tgagaaactt gttgttctaa ttctttgatt
     9181 agttggataa tatcatgttc ttccggtaac catttgatag tgcttcgctg atagagatag
     9241 taactcaagg catagacact gtttccatcg aatttgattt gataggcagt ttccatttgt
     9301 cgaaaggtct ctcggataga ttgtgtccaa taaaccagca gctcgtgttt ttcttgtcta
     9361 ctggtctcaa acagcaaaaa atcaaataat tgaatcactt cttgctttaa ttttccttcg
     9421 caagaatata gcagacagtt atttttcttg aaatcaatta ggatttgctc aaaactttta
     9481 acgattcgct gctgttcggg ctgttgcttc tccattaatt gctctaaggt ctggtcttgc
     9541 gtaatataga ttccttgctg tgctgtattt ttagtaatag gctgcgcaag tagttctttt
     9601 tgcaaatgat ggatcgtgac atggatttca ggctgttcac gctgttcagc atatgctttt
     9661 gctgctgaaa ctttgacgat atttttcagc tctcctatgt ttccggataa tttctgactt
     9721 gttaaaagtg acaaagctgg tccactgact gacaaagggc tccccatttt tctttgttct
     9781 tcgagaaaaa aagagtaaat cagttctagc cgttcattac gactacgctg attaagtgca
     9841 ggtagttcga ttcggatagg tattcttcga atgaaagttg ttaagaaatg actagtaatc
     9901 tcttcagtcg tagcaaaaaa gagacgtgtt ttgatccggc gtgacaaatt aggttctccc
     9961 atacgataaa tcacaccttg atcaagatag gtgaacagtt tttcctgacc ttctggtgtt
    10021 aagcgatgta cctcgtctaa aaacaagatc ccaccatctg ctgcttcaaa agcccctttt
    10081 ttattttctt ctgcaccagt gtacgctccc ttaacatgtc caaacaaatt gctagtcaaa
    10141 agttcaggat tatctgcata ttgtgcacaa ttaaaagtga tgaatggtgc agaatcttct
    10201 agcagatcat gaagcagaca atactgatag accaaattaa ctaaataact tttacctgtg
    10261 ccactttcgc ctgtaatcaa aagtggtaat ccgccatctg gatagtaaag agctgttttg
    10321 atttgttcga tactttcttt caaactggcg tcgtatccga tcataaaaga gaataagtct
    10381 tttggttgcg cgaaaaaagg ctgttcctct ttgagatcat cgatagaaaa ataaaaattc
    10441 gtgcgtaact taaaaaattg tttttcaaaa gcttctttgt ggaaataaat cactggtcga
    10501 gaattgattt tgactagtag ttgctcatca tttaactggt tcaaataatg actgactgtg
    10561 tttcttttta ccccaaaatg atcagctaac ctttgtgctg taaagacctc gttcatatgt
    10621 tctaaatcga aaaaggctgt ctggttctct aagtaacata aaatttcctt cttcaacaac
    10681 ttattcaccc ctcctccatc atagaaaacg gattcatttt gtcaataaaa tctttaataa
    10741 agaattgaaa aatatatctt attatagaca gcagcctact tatcccttat aatttttatt
    10801 ctaaaaaacg tacagttcct tccagttttt atactggaat ttcaacatat gaaagaataa
    10861 ggaggctatg actattcaat gaaatcggat aaatataaaa acaggaaaca tacttcctgg
    10921 aaaacttttc gagaaatcat cggatcttat ctcgctgtag ttgtattttt gcctttgctt
    10981 tatatggcta tgacagaaca aactcgtttt attggcgtag atacgcaata tatagccgaa
    11041 acaggatttt tattcatctt tatcttgatt tttgcgttct ataaatcctt ccatacaaaa
    11101 taagattcac agatttcaac tttcctgtct gtttttagct attacaaatc cttatatgtg
    11161 gtataacaac aatttattgg cataatgagg aaaaacgagt tatgatgctt tcagagagta
    11221 aagaggtact gttgtcacca tcctttttat tgattacgta caccactctt agttttttcc
    11281 cattgattac gaaaacaagg tagaaacatt gatgttccta ccttgttttc tttttttatt
    11341 ttcagatcaa aatctcaagt agcttttgac ttgatgtcgg gaggttgtct tcgttccatc
    11401 aaacctgata gaatctgcgc caaaccatag ttttctccac tattatttat tcctctaaga
    11461 tagtacccac aataaacgaa taaatatttt tctcattttc atactccttc tttgaaaatc
    11521 aattagattt ctctcaaccc acttttttac aaaaattacg tagcaaaaaa g
//
LOCUS       DAJSVD010000087        11515 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSVD010000087 DAJSVD010000000
VERSION     DAJSVD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.089"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(79..2154)
                     /locus_tag="ON498_002108"
     CDS             complement(79..2154)
                     /locus_tag="ON498_002108"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3940350.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ON498_002109"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="ON498_002109"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3940351.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ON498_002110"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="ON498_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3940352.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="ON498_002111"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="ON498_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3940353.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="ON498_002112"
     CDS             complement(5326..5721)
                     /locus_tag="ON498_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR3940354.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ON498_002113"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="ON498_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3940355.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ON498_002114"
     CDS             complement(6198..8390)
                     /locus_tag="ON498_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR3940356.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ON498_002115"
     CDS             complement(8414..9385)
                     /locus_tag="ON498_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3940357.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="ON498_002116"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="ON498_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3940358.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3112 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaaa
//
LOCUS       DAJSVD010000088        11323 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSVD010000088 DAJSVD010000000
VERSION     DAJSVD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.090"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            632..1603
                     /locus_tag="ON498_002117"
     CDS             632..1603
                     /locus_tag="ON498_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3940359.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1681..1917)
                     /locus_tag="ON498_002118"
     CDS             complement(1681..1917)
                     /locus_tag="ON498_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3940360.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1886..2017)
                     /locus_tag="ON498_002119"
     CDS             complement(1886..2017)
                     /locus_tag="ON498_002119"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3940361.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2093..2476)
                     /locus_tag="ON498_002120"
     CDS             complement(2093..2476)
                     /locus_tag="ON498_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3940362.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2509..3294)
                     /locus_tag="ON498_002121"
     CDS             complement(2509..3294)
                     /locus_tag="ON498_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3940363.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3472..4572
                     /locus_tag="ON498_002122"
     CDS             3472..4572
                     /locus_tag="ON498_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3940364.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4562..5386
                     /locus_tag="ON498_002123"
     CDS             4562..5386
                     /locus_tag="ON498_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCR3940365.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5383..6198
                     /locus_tag="ON498_002124"
     CDS             5383..6198
                     /locus_tag="ON498_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3940366.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6195..7025
                     /locus_tag="ON498_002125"
     CDS             6195..7025
                     /locus_tag="ON498_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940367.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7032..8063
                     /locus_tag="ON498_002126"
     CDS             7032..8063
                     /locus_tag="ON498_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940368.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8170..9443)
                     /locus_tag="ON498_002127"
                     /pseudo
     CDS             complement(8170..9443)
                     /locus_tag="ON498_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9670..9855
                     /locus_tag="ON498_002128"
     CDS             9670..9855
                     /locus_tag="ON498_002128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940369.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9979..10695
                     /locus_tag="ON498_002129"
     CDS             9979..10695
                     /locus_tag="ON498_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCR3940370.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10597..11117)
                     /locus_tag="ON498_002130"
                     /pseudo
     CDS             complement(10597..11117)
                     /locus_tag="ON498_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3185 a         2150 c         2604 g         3384 t
ORIGIN      
        1 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
       61 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      121 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      181 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      241 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      301 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      361 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      421 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaaa
      481 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      541 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      601 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
      661 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
      721 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
      781 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
      841 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
      901 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
      961 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1021 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1081 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1141 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1201 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1261 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1321 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1381 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1441 acactcacta gctgcagcca gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1501 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1561 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1621 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     1681 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     1741 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     1801 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     1861 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     1921 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     1981 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     2041 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     2101 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     2161 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     2221 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     2281 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     2341 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     2401 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     2461 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2521 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2581 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     2641 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     2701 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     2761 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     2821 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     2881 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     2941 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     3001 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     3061 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     3121 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     3181 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     3241 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     3301 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     3361 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     3421 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3481 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3541 ctgacaggct gcggctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3601 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     3661 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     3721 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     3781 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     3841 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     3901 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     3961 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     4021 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     4081 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     4141 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     4201 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     4261 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     4321 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     4381 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     4441 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4501 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4561 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4621 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     4681 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     4741 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     4801 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     4861 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     4921 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     4981 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     5041 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     5101 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     5161 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     5221 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     5281 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     5341 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     5401 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     5461 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5521 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5581 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     5641 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     5701 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     5761 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     5821 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     5881 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     5941 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     6001 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     6061 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     6121 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     6181 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     6241 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     6301 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     6361 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     6421 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6481 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6541 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6601 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     6661 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     6721 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     6781 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     6841 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     6901 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     6961 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     7021 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     7081 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     7141 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     7201 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     7261 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     7321 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     7381 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     7441 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7501 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7561 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7621 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     7681 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     7741 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     7801 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     7861 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     7921 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     7981 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     8041 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     8101 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     8161 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     8221 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     8281 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cgcttttttt
     8341 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     8401 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     8461 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8521 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8581 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     8641 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     8701 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     8761 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     8821 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     8881 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     8941 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     9001 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     9061 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     9121 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     9181 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     9241 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     9301 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     9361 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     9421 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9481 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9541 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9601 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
     9661 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
     9721 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
     9781 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
     9841 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
     9901 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
     9961 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
    10021 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
    10081 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
    10141 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
    10201 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
    10261 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
    10321 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
    10381 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
    10441 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10501 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10561 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10621 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    10681 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    10741 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    10801 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    10861 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    10921 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    10981 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    11041 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    11101 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    11161 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    11221 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    11281 gtctgtttgc atttattaaa aaaaagaaaa agtattggca aca
//
LOCUS       DAJSVD010000089        11245 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSVD010000089 DAJSVD010000000
VERSION     DAJSVD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.091"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            212..1081
                     /locus_tag="ON498_002131"
     CDS             212..1081
                     /locus_tag="ON498_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3940371.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            1101..1448
                     /locus_tag="ON498_002132"
     CDS             1101..1448
                     /locus_tag="ON498_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940372.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1461..2723
                     /locus_tag="ON498_002133"
     CDS             1461..2723
                     /locus_tag="ON498_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940373.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2803..4179
                     /locus_tag="ON498_002134"
     CDS             2803..4179
                     /locus_tag="ON498_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCR3940374.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            4176..4805
                     /locus_tag="ON498_002135"
     CDS             4176..4805
                     /locus_tag="ON498_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940375.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4824..5303
                     /locus_tag="ON498_002136"
     CDS             4824..5303
                     /locus_tag="ON498_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940376.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5805..5858
                     /locus_tag="ON498_002137"
                     /pseudo
     CDS             5805..5858
                     /locus_tag="ON498_002137"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5862..6461
                     /locus_tag="ON498_002138"
     CDS             5862..6461
                     /locus_tag="ON498_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3940377.1"
                     /translation="MREKNKNSSRYLVSTVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6477..6953
                     /locus_tag="ON498_002139"
     CDS             6477..6953
                     /locus_tag="ON498_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940378.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            6970..7872
                     /locus_tag="ON498_002140"
     CDS             6970..7872
                     /locus_tag="ON498_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940379.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7945..8136
                     /locus_tag="ON498_002141"
     CDS             7945..8136
                     /locus_tag="ON498_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940380.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8300..8887
                     /locus_tag="ON498_002142"
     CDS             8300..8887
                     /locus_tag="ON498_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940381.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8887..9786
                     /locus_tag="ON498_002143"
     CDS             8887..9786
                     /locus_tag="ON498_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR3940382.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9786..10700
                     /locus_tag="ON498_002144"
     CDS             9786..10700
                     /locus_tag="ON498_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940383.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10752..10967
                     /locus_tag="ON498_002145"
     CDS             10752..10967
                     /locus_tag="ON498_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940384.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4315 a         1510 c         2267 g         3153 t
ORIGIN      
        1 ttttttttga taaaaaaact agctattttt ttaaaaataa tttggttcaa aatttatttt
       61 tttaacttga taattttttg ccatcctttt ttaaattggc tatatatcaa ggtttatagt
      121 ggggataaat aaaaatacat ttgacaaaat ttacaatata gtttattata tatttatctt
      181 attatgataa cacaataaaa ggaatgatgg tatgaaaaag aagaagttta gtcgatcgtt
      241 gttaggaatt ttagttttag ttgcggttgg tcttagtggc aagggttact ttcagaataa
      301 cagcactcag gaacatgatt ttcagatcaa aagtagtgtt caggtaggta ataaagcaga
      361 cagtgaatca aaagaatttt tagggatacg ttatgatcca aacaaatatc caaataatat
      421 cgtaaacatt aatgatggga aatatacact gacaaaagaa gaccatgaag cattatcaga
      481 aaaaggcagt caaacctttt gggtaaatta tgcagattta gatcaacttg gaagagcagg
      541 agatgttgaa gcgttgatca cctatgatgc tataaattct cactcatcta aaaatgtgga
      601 acgtccatct ttcaaaagtt ctgtccatat tgcaggagaa tacaaggatg gagtatttga
      661 tcaagagaga gatacttggg tcggcaaaga ttcaaacaat gaaatcactc agctagaagg
      721 atataaaggt tatctctata acaaaagtca ttcattagca tggtcgctag gaggagatat
      781 ggaggtacac aatttaacac ttggaacgag agcgcaaaat gttgggaaaa atgatcagaa
      841 tggagggatg gcttactcag aaacgattgt acgtgatgca atctataaaa ataagaatga
      901 aaaagtattg tataaagttg atccaattta taaagggaat gaattggttc ctcgtggttc
      961 tcatgtcgaa gctttctcag ttaacgataa tggaaaaacg gtaaacctta atgtttatgt
     1021 atttaacaat caaaaaggaa tcaacatcaa ctatcaaaat ggtacttggg taagagatta
     1081 gaaattgaga aggtgaagaa ttggaaacaa taagcatcaa tggattagat aaatcagttg
     1141 caaatcgaat aaaaaaagga aatggctact atcttctgaa gaagggtaaa tattcagttg
     1201 ctagagagtt agaaacaatg agtatcgtgt atgtagctaa gaatcatttt gacgtagaac
     1261 aggctatttt tgatccagat tctgaaatag ggaaatgtgt tgaattatca gagaaaactg
     1321 ttagtacaaa aggggcgatg atcattgaga gagaaatgtt agacgaaatc agagtaatca
     1381 ttcctaaaga tacgcagatg tgtgaggaca gcataagtga cgatgaacca aaatcagaaa
     1441 gaaaataagg agatattcag atgagcgaaa aatccattga attaaaggaa tataagcgaa
     1501 gcgatggaga aaaatattat cctgtagcca gtttttctaa atcagtatat ctggttggac
     1561 gtgcattggt agttggtgca attggcatat atattttgca ttgggtaaca aaggttgtac
     1621 aactgcttgt agatacctta gtaagtattc ttgtctcgtt aaatgatgtt aacggaacag
     1681 atgatttagg attaaaagag atgtttaaag agttactcaa tgcgtttttt cccaattatt
     1741 taagttattc atataatgta caaagctttt ttgcaaagct acacttggta gacatctcta
     1801 ttttagtatt agaagcagga acggtattct ttgttggata tttggttact ttgatgttca
     1861 ggcatcacta tggagaacaa agaccatttt ttgatgatat ggaaagtaga aggttgaaaa
     1921 gagaagctct agaagctatg gatgtgaagt atgatccaaa caagattata ggagatgaaa
     1981 cgaacaacaa aaaaagaaaa cgaaacaaaa cgcaattaga atccgatgtg ctatcaacgc
     2041 ttaaaaatat caaaatttct gttcatacaa gacgtgatct aagtgtcaat gaagatattc
     2101 gagaatatat cgtaaaaatc ccgcaaccaa aagggaagaa agttagagaa acagttttac
     2161 gacaaattca agatttaggg tcgatcctga caagagcaac aagcggacga gccaaatttg
     2221 gtgaaaaaga agatgaagtt tcaggcgatt atttcatttt ccgagcatca gtaaatattg
     2281 aaagagaacg taaggaaaga gtaagtacca aaaacaaaga aacggaaaga gcaacatctt
     2341 caatcgcagt ttcaaattca caattttctt ttccaatcaa tctctttgcc gataattcca
     2401 aaaatatcca aaaccaaacc gaaaaagcta gaaaatatgc cgaaactcaa aagcaatctt
     2461 tagaacttta tctatcttct gctgacgtac ctgcaagtct aacagatatg cacgttggga
     2521 atacatctat tgaatatttg tttactttat ctcgtaccag aaaaattcaa ctaagctcaa
     2581 tgaaagaaga aattgaaact tacttgcata tatcaggtgt aatcgtgcgg atcaacgcag
     2641 ggcgtattca aattacttta ccattaggag aaaatgatcg tattccaatt gatgtaccat
     2701 caatgatcaa tagcgtgttt tagaagacat cacaaataga aacagaaaat acaaactacc
     2761 agttcacaaa cagaaaagta ccgagaaaag tgaggaatag aaatggctat gaaattatta
     2821 gagaaagtca aaaagagagc cgaaaaagct gaatctattt tgctagataa agaacctaat
     2881 ataaagctta atggaaaatt cagtgtcaat atgttgaaca caaaggctaa agacccaaca
     2941 gatgctattt ttggaattaa gcctgatggc acaattgctc atttttgcat cagcgaaatg
     3001 ccacacatgt tggttgcagg aacgacagga tcagggaaat cagtattact gaatgaaatt
     3061 cttgttacag taatgtgtca ctcaacgcct gatgaattaa aaataggaat tgtagaccca
     3121 aaaagggttg aatttggaag atacaaaaaa ttgccgttca tgttggctga tccaattaca
     3181 gatatggatg aagcttatga tttttttgaa tatcttgtga tcttgatgca tgaacgttac
     3241 aaactgatgg aaaaagcagg gttacaaaac atcacgctat ataacgaata tgccgaaaaa
     3301 aatggattag atagatttcc ctatgtgatc ttattagttg atgaatattc acagttggtt
     3361 ggaacacata aagaagtaga aggtttgatt gttcaattag gacaaatggc aagggcagca
     3421 ggtattcatg ttatcttagc aacacaatct cctcgatcaa cagttgtcac tggtattatc
     3481 aaagccaatt ttccgtcacg tgtttgcctt atggtagcaa gtgatttaga aagtaggatt
     3541 gtattagatg aaggcggtgg agaaagtcta agtccaaagg gagatatgct tattaagctt
     3601 gttgatggaa gtatggttag agcgcaggga gcttacatca gtaacaaaga aattgaaaca
     3661 atcttcaatc accttagaaa tactatgcca gaaccagaat atgttgatta ccgagcaatc
     3721 gtagctgaaa ctcgtggtga gggcggtgaa gacgaggatt ttggtggaat tggtaaagct
     3781 cctgtaagaa aatctgctaa aagaaatttg aacacacctt tagcaaatcc atcatctaaa
     3841 gagccatcaa ttgaaaagaa gagtgcgttt gctattaagc ttgaaaagaa caaagaagaa
     3901 aaagtcgagc agggagctaa aacactagaa aaactgcaag cagaaaaact agaaaaagtt
     3961 ttaagggcat taggaaaagc tgatcctgaa ccgcaaacct caaacaacac gaataataaa
     4021 cagccgattg catctaaaac caaacctaaa gctaatgatc caaaaggctc agaaaacgta
     4081 gtgatcacaa ctgataaatc tgaaaagaaa caaataaatg gaagtacttc acctatcatg
     4141 caaaaaatgt taaaagcaaa aaaacaggtg cataaatgat catcgtcagt tcttttttaa
     4201 gatggcttac tccatataaa tatacagatg aagtagttgt gaaacgagcg aagaaaagag
     4261 ataaaaaagc ggtactaact cctaaacaga tgcttgatca tggcatttta ggggtgaagg
     4321 ttttagaagt gaataaaccg tttgaatcaa atagtgcaag agaaaagttg cttggagctg
     4381 aagccgatgt cgcatatttt taccatgaca aaagcaagca tcaaatagct attaaattca
     4441 atccaaaatc ggcatggcgg tatcaagcaa gagatgactt gaaacgtagg aaacttagac
     4501 caatgctcta tccagaagga aaagaagcat ttgatagaac ccacatcgtt cccattggtt
     4561 ttcatggtac agaatcagat aaccgcctat tagtaggttg gaacagcaat caaaatagag
     4621 ttcagatcaa taaatttgag aagaaagtaa ggaaattcaa cgatcgaaaa acgatcctat
     4681 ggtttgcaga tatttgtaaa cagccggatg gaacagcaaa gtgggaagcg gtgatatttg
     4741 acgaaaatgg taaaaaagta atgtctgaaa catggcatga taaagctaaa tttgtttggc
     4801 agtagcagta gaggtgaaaa agattgctag aactttttga agcaaataga acaggcttta
     4861 atcaaaagtt agaagtggga gaaactatag aatataaaga taaaaactat attattatcg
     4921 gaatctataa cacgagaatt aacagaatag gtgatccacg tataaccagt gacttggttt
     4981 gtcaatctgt taactattca cctgatctga caagcaggta taagaaatac gtgttattag
     5041 aatatagaca caagattact gatgaaattg gtgtaaacaa cacacgaaac atatttaaga
     5101 tcggaacagt gattccttat agtaacaacg aagaaaaaat attttatcag atttacggaa
     5161 tagaaaagtt tgaatatgaa catgtcgatc tgctagtgac gtaccaaatg cgtttaatag
     5221 aaccttggtc acaggcagaa attgataaag cagtaaaatt gaaccgatta tcaaaattta
     5281 acgtattagg aaatgccttt taaaaatcgc aatacacaaa cagaaaaata ccaaaaaatt
     5341 agcaatgaac gtaggtaaga acatgacaat aaataataat aaccctaaaa aatataatgc
     5401 tcttgtccaa tgctatatcg aaaacaaaat tacaatgcaa gaagtcgctg atgaattagg
     5461 caaatcaaag agcctagtat cacacgattt taagcacaat gtttttccga acccgattga
     5521 gctgaggaaa aaatttcaaa agagagttta tcaagaaata ggtaagtgga ttgatgaagg
     5581 gaatgcaatt gaccctttaa ttgaaaaata tcctttttta aaaagcaaaa ataaaattca
     5641 aaggttaatt cgtcaaggcg atattcaagc taaagaacct gttttgacaa ccaatgattt
     5701 ggctaaaata aatcttcata aacaaattat tgaagacttt gaaaaaaatc tttcaacaac
     5761 tgaaatttta aaaaaataca atattccgag aggaaagcta acatcgactc tttttagaga
     5821 atatggagaa tcggcagtta ctaaagcaaa ggaagtagcg aatgagagaa aaaaacaaaa
     5881 atagtagtcg atacctagta tcgactgtag tttttgtttg tgtattgatt ttcctgtttg
     5941 attttttgca gattggaaaa agcataattg gaaatacatt gagtgcaaag gaaatggttg
     6001 ctttaggtgg tttgtatgaa ggacacttgt tcccgaatat attcatttct atcgtttctc
     6061 atattaactt gtaccattta atttctaatt tattgttctt agtagttctt gaagaagcac
     6121 tgaatgatca gtatgagtgg ttaacattat tgatcgtttt aatcatatca ggatttctag
     6181 gaagcttagt tccttttctg ctacacagca acatcgtaag tatggggctt agtggaggta
     6241 tttttggatg gattgcttta tttgcgattg catacatccg ttttgatagc aagaaacatt
     6301 tattactaag tgtttttgta ttattgataa tcggcatttt attggtatct acttttgaat
     6361 caggcgaggc aaatgtcttg tcacatgtaa tcggatttgt ctgtggaatg atcttgggat
     6421 ttattctaaa gaacgtttca ttgatcaaac aaaagaatta aaagggagtg ctaaaaatgc
     6481 caaaaagaaa agaaaagaaa attccagtgt ttttgtctgt ctcagaaatg ttatcacttc
     6541 aaatactgtt aagaaaaaag attgaaacaa tagacgaaga agttagaaat aaaagggcta
     6601 tgtacgaaca acgtgaagcg ctaagagaac gaatccacta ttttgatgat atacacacta
     6661 aaatctatga agcacgtaca aatcacgaag acgaaagtaa attaggatta gctcaacaac
     6721 ggatacttga atggcttaat acgaatactg atcaagaacc gttaagagtg attagtaatc
     6781 tttttgaatt tggaacgaag cattggttta atgctgtaga gaaggatgct gacttacaag
     6841 aagcgtttga tacgctaagt aaggatagca aatccatgcg agatttattg attacttatc
     6901 taaatgagca tgagtttgaa caaacgaaaa ccaaaaagaa aaataaagaa tagaaagttg
     6961 tgatgaatca tgctattaac cctgttaggt tatttatgtt taattcttgc tttttttggg
     7021 gcaatcgcta taattattgg tcaatttcaa acaaataatc cgattatgcg taacataggc
     7081 attggaattg tgattgtaac ctttattgtg tttaatgcca ctaagatttt tggaagtaac
     7141 gataaacaat ttggtatatc gggagttaat aatagttatg ctccagattc tttgggaagg
     7201 gtagtaatag atggaacatt tgaaaatgta acgagttata aaaatgtttc gctgacagtg
     7261 aaggaagatg gcaagaaagc atcagacgac gttctcgcta ctattaagcc gtctcaatca
     7321 caatttgaag tgttttatac agttaaagag aatagccctg tacacaagct gacattggtt
     7381 tttaacgttg atggagaaga atactataaa gatgtaaaaa taaataaaac atcaacgaaa
     7441 aagattatcg aatcagcgaa aaaagaagta gctacagaag aaagtacaac tgaatcaagt
     7501 tctactgtcg aagcggaact accagataaa attgattttg atcctgaaaa agtcaaatcg
     7561 tttgaatctg tttctggttc tatggctgat cggtttaaca ctgaagtctt caaagctatc
     7621 aaaactgtta aattggatac tgaaaagcaa atgattaatg ttaccttatt caattcagtt
     7681 gaatctatgg atcaggataa aaaagttacg gttgcaagag aactaggaaa aagagtaatt
     7741 ggtttgtatc gtggtactgc taaaccagat tcagacggtg cagatttgcc agttcatgta
     7801 gtttatgaag acggtaaagt ttttggagat tctacagcaa acgaagcatt aacgattaaa
     7861 ttggctaaat agccagaaag ttataataaa caaatggtgt cacaccaata atatataggt
     7921 gtgacacctt ggagatgata gctgatggta gtaaataaag aaaaagttag aaagtatgtg
     7981 actttaaaaa tagcttctgt taaatattta gaaaagcgag ctaaagaaga agaaagagca
     8041 agaggtaaga aagtaacagt ttcagagttg ttagaagaat tgatcgagaa tgatcaaaca
     8101 ataagacgtg catttactaa taggtctatc agataacaat ataattaata aaaaagaaag
     8161 agtgatttgg tcttagacta aatcactctt tttatataaa gtttctttac actcattccg
     8221 aataatgtta aaattcttta tatgatatat agttccttgt cagaggaaga gataaccagc
     8281 aatattggag ggataataag tggaaaacaa taatgatatt agacatttta gcattgggga
     8341 aagagttaca acagtaagtg aattatcaaa ggattataga gataaagagg gaacgattat
     8401 ttctcttaat aagaatcgaa gaggcgtttg gcaatatgtt gttgatttag gtgatggcgt
     8461 tacagttgag tgtaaagata gtcaactgat cagaaataat ggcagtcatt ccattgaaaa
     8521 agcaattctt gtatacccaa aagtatcaag taagatcatg aatgactggt tgcttggaaa
     8581 atttggtgag tacaataaaa aagaaatcgc agaagtgatc ggcaaatctt atccaacagt
     8641 attagggtat ctaaaaaaga taacgagtaa agaagcaaaa attgaggatg ttttaacagt
     8701 aagagagctt caatcattaa tcgagtatcg gaataaacaa ataacaaatc ctgagtacct
     8761 tagaaaagaa gatggtctat tgaaacaatt ggaacaagta aataaaatcc ggaagatgca
     8821 aaaggtagct cctttaagcc ttgatacctt taaaaaaatc tatttgaaag agatcaaaaa
     8881 agactaatgt ggactgtaac tttaccgttc caacctgtat ctgcaccaag accaagtgca
     8941 agaaggaatg ctgctgatga aactaatgac gtagcagaga aaaggatttc aacctactat
     9001 gataaaaagt atcttgatta tttgaaaaaa ataaagagct ttatcgaaga tcaagattta
     9061 ttagatgatg aattttactc gattgtaaat gatcctatgg gcgcaattat ggaagtggac
     9121 ttttactacc agataccaaa aagctataaa aaagtctata acattatgaa gacaacagcg
     9181 ccagatttag ataatttagt aaaaagtgct atggatggta tttttaacat ctcagctata
     9241 cgagatagcc atatcacagg cttaattgct tttaaatata atgtggcgaa cgaaggaaaa
     9301 acagttgtga ggatcaaacg atattcagaa tttgagtggg atacttctga aggattgaac
     9361 ggagatatat gggaagctac atttgatttt aaaccagtag caacgccaag accaaagtct
     9421 aaatttagag gaaatgggat tatcacttac tatccaaaag agtacaacga ctatttggaa
     9481 aatatcaaga atacgcttaa agagaaagat ttggtaaatg atcaactgaa aaaggtaatg
     9541 aatgctccta tgggggttat agctcagata gattattttt accaagtgag aaaagataag
     9601 aaaaagcttg attcactgat gcgtacttca cagccggata ttgataattt agtcaaagga
     9661 acattggacg gaatttttaa caaggaaata ggcatcaaag atagcagggt tgtagggtta
     9721 attgctttta aatacaatac ttttgaaaag tcgaaaacaa acgtgagact acaggagatg
     9781 ggctgatgaa taataatgaa aaaattcttc atgtacttga tagctttgaa ataattcaag
     9841 aagaactaaa aaaatatcgg gatgttttgg aacaaagata tgattttgtc aatcagcaaa
     9901 agagtaatca catggatttc attctcaata tgaatgactt gaagaagaaa ttagttgaac
     9961 gaaaagaaca agaaaagttg atcaaagctt actttgaact cggtgaaaaa gaagttaaaa
    10021 atgcaatgga actgaatgaa gagcgcaggg ttttagatca gctgttagaa caattattgg
    10081 tgatgtttca aaaaggacga atagatgagg atttaattga agaaggattg agaaaatatc
    10141 cttcaaactc aggtatcgga atagtgctga aagcgattga tgaagaagaa attgaagctt
    10201 tcataccgcc agaggatttt gaatcggcaa tggaatatat aaaatattat agccaaggga
    10261 tcactgcttt tagagagttt gatcctgaag atgttattca tgacttgaac aacctaaaag
    10321 aatggtgtga aagttatgga gtcgatgatt ccggattgga ctacttgatc agtattatgg
    10381 aaatcgaaga agagatgccg gataagcctg atccaacaga tattttagaa ttgatccatg
    10441 aagctagaaa tccaattgcc tatatttcta gaggatatac tgtattggaa tactataagc
    10501 cttatatatc agcgatgaac catttaagac gtgtattaag ggaaaaacgt gaataccgct
    10561 cagtgttgaa tgcaacgaat agattagaaa aagctgtttc agagctagat gcttattatc
    10621 gtgaacatta cctgcaagca ggtggtatgc caagaaatac gaaagcaaat atcagtaggt
    10681 atataaaaaa agctgagtag gcggagttag cccataaaat ggcaaaaggt aacctgaaag
    10741 gggcattttg aatgaaaagg gatgtttttg aattgtacgt attagaacgt ctagaagacg
    10801 gcatgatggc acaagagata attgatgaaa ttgatgatat gatcagcaga tttggtcaat
    10861 tggagttgaa tgatggaaac aaaaattcgg tagtgctaaa acaattttgg ttgaacaatt
    10921 tttcgtataa ggattttatc aaagaaaagg caaatacatt gtactgacta aattcacgag
    10981 gagcagtgat gagagcttat tgctaagagc agttaggctc ttttttgttt gtgtaaaatt
    11041 atttgtttta taatacacat ttatacacaa aaaaatattg acagatagta cacaaatgtg
    11101 gtaggatata tttgtaagca agttagatta acaagttaaa ggaagagcag ttatgaaaaa
    11161 agaagaacta atagatatat ttcaaattgt tgaacgagca aataatatgg gaattatgtt
    11221 ttttgatcgt atttctttaa agatg
//
LOCUS       DAJSVD010000090        11167 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSVD010000090 DAJSVD010000000
VERSION     DAJSVD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.092"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            129..1388
                     /gene="lysA"
                     /locus_tag="ON498_002146"
     CDS             129..1388
                     /gene="lysA"
                     /locus_tag="ON498_002146"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3940385.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1375..2175
                     /gene="dapF"
                     /locus_tag="ON498_002147"
     CDS             1375..2175
                     /gene="dapF"
                     /locus_tag="ON498_002147"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3940386.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2178..3059
                     /locus_tag="ON498_002148"
     CDS             2178..3059
                     /locus_tag="ON498_002148"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3940387.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3056..3772
                     /locus_tag="ON498_002149"
     CDS             3056..3772
                     /locus_tag="ON498_002149"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3940388.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3778..4473
                     /gene="dapD"
                     /locus_tag="ON498_002150"
     CDS             3778..4473
                     /gene="dapD"
                     /locus_tag="ON498_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR3940389.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4489..5490
                     /locus_tag="ON498_002151"
     CDS             4489..5490
                     /locus_tag="ON498_002151"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3940390.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5487..6848
                     /locus_tag="ON498_002152"
     CDS             5487..6848
                     /locus_tag="ON498_002152"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR3940391.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6808..7959
                     /locus_tag="ON498_002153"
     CDS             6808..7959
                     /locus_tag="ON498_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR3940392.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7934..9103
                     /locus_tag="ON498_002154"
     CDS             7934..9103
                     /locus_tag="ON498_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3940393.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9278..10657
                     /locus_tag="ON498_002155"
     CDS             9278..10657
                     /locus_tag="ON498_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3940394.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10938..11167
                     /locus_tag="ON498_002156"
                     /pseudo
     CDS             10938..11167
                     /locus_tag="ON498_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3724 a         1949 c         2497 g         2997 t
ORIGIN      
        1 aaaaaaatat ttcgaaatat actagacaat tcagaaaatt ttgtttaata ttattccaat
       61 aaaattcaaa tctttatcaa atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga
      121 atgaaaaaat gacagaattg ataattggcg gagtcacagc aagcacattg gcagaaaagt
      181 ataagacacc tctgtatgtg tatgatgaag aaaagatgag acaaacgatg ctgtcattca
      241 agcgaggctt tgtatcgaaa cgttttgata caaaagtgct ttacgcctct aaagcattcc
      301 aaacgattga gatgctgcat ttagttcgtg aatatggatt cgggctagat gttgtcagtg
      361 gtggggaaat ctataccgct ttgcaagctg attttcctag agatactatc tacttccatg
      421 gtaacaacaa gacgccagag gaactgatct atgcacttga aaatgatttg ttgcatattg
      481 ttgccgataa tttgatggaa gttgaattat tagcagaatt aagccaacgc tatttaaaaa
      541 aaatctgtat catgcttcgg ttgaatgtcg gagtagaagc acatactcat gaatatatcg
      601 tcactgcgca tattgattca aaattcggca tgagctatga aagtaaagag tgccaacaag
      661 cccttcagtt gataaaagaa agcagctatc tagagttaga aggatttcat gctcatatcg
      721 ggtcacaaat ttttgatgtt actgcttggt tagctgaaat cgacaaattg acgaattact
      781 taaccgattt tgaccaacct ctttccttga atcttggcgg agggtttggg attcgttata
      841 cacaaaatga tcagccgatc ccagtcgaag aggcactaaa aaaactgatt gaatatacgg
      901 aagaagcttt ggaaaaacga aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa
      961 gtattgttgg agaagctggt acaacactat acacgattgg tttcatcaaa aagacaccac
     1021 ataaaaaata ttactttgta gatggcggga tgacagataa tatccgtcca gctctgtatc
     1081 aggcagaata cgattgtgat ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga
     1141 cgattgcagg aaaaatgtgt gaatcaggcg atgtggtaat taaagaagct tatttgccga
     1201 aagcagaatc gggtgatctt ttagctgttt attccacggg agcttatgga tactcgatga
     1261 gcagcaacta caatcgagcg acacgaccag cagttgtttt tgtcaataat ggccaatcaa
     1321 gactgattgt tcgcagacaa gcattttctg atctactaag gggggaagtt tcctatgatc
     1381 cttcataaat ataatggctg cggaaatgat tttatcttat tagattacgc agaagacaca
     1441 gattatagtc aattagccgc ttctttatgc acaaaagaaa aatatgatat agacggattg
     1501 atcgccgtaa aaatcgatcc gttggaaatg atctattaca acaaagatgg cagccgcgcg
     1561 ccgatgtgcg gaaatggcat tcgctgtttt gctcgatacg taaatgatca gaacatgatg
     1621 aatgaacagg aattcaacgt agagacatta gcaggcacaa gaaaaattac ggtgttggat
     1681 ccctcacctt tttattgcag cgtagatatg ggagtgccag atgattctcc ggaaaaaatc
     1741 aaagctaatc aaacttatcc aataaaaaac caggaaatag agatcaatgg tgaaaaagta
     1801 acgatcacta gtttattttt aggaacgatt cataccgttg tttttgttga aaatgcgcgt
     1861 gaggaagtct ggagaaaaag aggtgaactg atctgtcatc atcctttgtt caaagaaaaa
     1921 accaatgtca actttgtaga agtagtcaat gaacaagaat tgatcgtccg tacctatgaa
     1981 agaggagtag gttggacact tgcttgcggt acaggttgct gtgcctctta tgtggttgct
     2041 aaggaaactg ggaaaatttc agcagatcag gtagttgttc acttggaaca aggagacttg
     2101 atgatctctg gtgaggatgc gatacaaatg gctggcccag cagttcatga atggacaaga
     2161 atattggagg aatgaagatg ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg
     2221 aagagggatc agtagattat gctggtttgg aaaaattgat ccaattccat ttagatgaac
     2281 aaacagatgg attattagtt ttaggtacaa caggagaatc ttctacgctc acacaatctg
     2341 aggaggagca gatccttcaa ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg
     2401 caggagctgg aacgaataat acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt
     2461 tgggggcaga cgcacttttg gtcatcaccc cttactacaa caaaacaagt gatgcaggac
     2521 tagctgctca ttttacagca attgcagaag cctcaccgat tccgcttatt ttgtataatg
     2581 ttccttctag aacaggcatg tcgattccaa tccatgtatt agtaaattta gcagaacatc
     2641 ctaatatcat tggtttgaaa gaagcctcag gagatatggc ttatgtgatg gatgccgctc
     2701 ggttgattgg agaagaattc ttcctttaca gtggaaatga tgatttgatc ctaccagtaa
     2761 tgagtgtagg aggaagcggt gtaataagtg tatgggcgaa tatccaacct aaaatagtcc
     2821 atgaattagt aaaagatacg caggacggaa gatggcagca agcaaaagaa aagcaattaa
     2881 atgcacttga actgatccat gccttgttca gtgagaccaa tcctattcct gtcaaagcag
     2941 ccatgtcatt attagactta ccttccggac ctctacgcct gcctttagtt tctctttcaa
     3001 aagagaaaaa gaaacaatta gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa
     3061 aatcggactg attggcagtg gacagatggg aaatagagtc gaagaagttg cacaagaaag
     3121 aggagatacg gtccttcttt tacggacggc acctaaagaa gcaactacgc ttgcttttac
     3181 ggaactgcca gaactgctta ttgatttttc tcatccagat aatttagaga aaatcctttc
     3241 gtatagctta tcatatcaac ttccgttagt cattggcacg acaggttata cacaatcaca
     3301 gttccaagaa ataaaagaac atgccaaaca tgtacctgtg atgtatagtg ctaatttttc
     3361 gttcggcatc atggtgatga atcaactaat caagcaagca gctgcaatgt tgcaaggctg
     3421 gcagatcgaa ctgattgaaa aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc
     3481 aaatatgctg cttcaaacaa tccaagaagt gcaaaagctg cagcccgtat acaactggca
     3541 agggaaaaaa agagaagaaa accaaattgg tgtacacagt attcgtgcag gttctttacc
     3601 tggtgaacat gatgtattgt ttgctgctac tgatgaaata tttacgatca aacatgaatc
     3661 tttttctaat cgaatctttg ctgcaggcgc agtagaagca gcggactggt tgaaagatca
     3721 gtcaccaggt tattacaaat tagaagattt attaatggac aaaggggtgt aaaaaacgtg
     3781 gaagctcaag aaatcatccg ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc
     3841 tatctgaatg taaccgagcc aatcgaattt aaagaaagta aagtttttgg tgaaggaagc
     3901 agcttcgttg tttttggtga ctatgagacg attgctccgg ttttagaagc tgaatcagaa
     3961 aaaatcattg aaatcgaaat cgaaactgct gcaagataca gcgcagttcc tttgctagat
     4021 attaaaaaaa ttaatgctcg tatcgaacca ggtgctatta tcagagatca agtgtcaatc
     4081 ggcaacaatg ccgtgatcat gatgggagcg attatcaata ttggtgcagt aatcggtgaa
     4141 aacacgatga ttgatatggg tgctgtctta ggtggacgag cgacagtggg caaaaactgc
     4201 catattggtg caggagctgt cttagcaggt gtgatcgaac cagcatcggc caaaccggtt
     4261 atcgtagaag acggcgtact ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc
     4321 ggaaaagatg cagtcgtggc agcaggagca gtggttttag aagatgtggc agcagaaact
     4381 gttgtcggtg gaatacctgc acgtgtatta aaaataagtg atgatcagac aaaagaaaat
     4441 acagcattga ttgctgcgtt acgcgaatta tagaggagat ggagaaaaat gcgcgaaata
     4501 gatacctgta ttgtgggggc aaccggatta gtcggccaaa cgatgctcaa agtgttagaa
     4561 gaatatgatt ttccagtagg acgattgaaa ttattagctt caaaacgttc agccggtaaa
     4621 gtttgtgtat tcaagaaaaa gaaacacact gtacaggaat tgacagaaac ttcatttgaa
     4681 ggatatgatt tagcgctatt ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc
     4741 gcacgaagtc agggactatt tgtcatagat aattcctcag catggcggga agattcggat
     4801 attccattaa tcgttccaga ggtcaatctg gaagactata cattgaatcg gctgattgca
     4861 aatccaaatt gttcaaccat tcaagcggta cttccgctta aagctttaca agaagccttt
     4921 ggtatccagc gagtcaatta ttcgacctat caagcagttt ctggttccgg gcaaaaaggc
     4981 attgaagatc ttcatcgtac gcaaaatgac gaggaaccag ttttgtatcc ttataatatt
     5041 agccaaacgg tgattccgga aatcgatcag cctttggaaa atgggtatac aaaagaagaa
     5101 cagaaaatga taaatgagac ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca
     5161 acctgcgtac gtgtgccaat cgaaaatgga catggagtct ctgtggcagt tcaactgcaa
     5221 aaagagttta cgacggaaga aattagagag tgtctcagcc agtttccagg gattcaatta
     5281 gaagatgatc tagcagcaca taaatatccg acgagcattc ttgctagagg aacagatatg
     5341 gtttatgttg ggaggatcag aaaagacact tctttagaga acggattgtt gttctatacg
     5401 actgccgata acatacgcaa aggagccgct gcgaatgccg tacagattgc tgcagcttta
     5461 atgcagaaaa tagcggaggt ggcaagatga gtaatacgct gaaagtagcg aaattcggcg
     5521 gaacatcgat gtctcatgaa gagcaattca aaaaagtccg tacaattgtt ctttcagatc
     5581 ccaatcgcca agtgatcgtt gtttctgctt taggcaaacg tacgtcagat gatgacaaag
     5641 taacagactt actttatttg atttatgctt accttcagca tcatgttgat tggacacccc
     5701 tttggaaaag gatttgtgat cgtttcattc aagtaagaga tgttcttgcc ctgcagttac
     5761 ctattgaaga tgaactagca cgcatacaga aacaactgga agacgaaaag ataactgaag
     5821 attatttagt aagtagaggt gaatatctga ctgctcaact gatggctgag tatcttggtt
     5881 accagtttat tgacgcaaaa gacctgattt tctttgacta tgatgggaaa atcgatcagg
     5941 aaaaaacagc acaggaaatc acaaaagcat ttaaaagata tccgaaaatg gttgttccag
     6001 ggttttacgg ggcgaaccct gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca
     6061 ttaccggcgc gatactggcg aagatgttgc aagcggaaag ttatgagaat tggacggatg
     6121 tttcaggaat catgatggct gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa
     6181 tctcttttag agaactgaga gagatggctt atatgggggc taacgttctt catgaagcag
     6241 ctgttttccc agttcaagag gctgatatcc ccatccaaat aaaaaatacc aatgatcctt
     6301 tcgctcaagg gacaaaaata tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa
     6361 tagctggacg caaggatttt ctttccatca ctttattcaa acgtcatatg tccgatgaaa
     6421 tcgggtttat ctggaaagca atgagcattt ttgctaaaca tgagatatca atcgaacata
     6481 ttccttcagg gatcgacaat atcggagtga tcgtctcagc agaagcgatt gccgataagt
     6541 tatttctcat cacgaaagaa ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg
     6601 aagacttagc tttgatctct gtcgttgggg gtccgcataa agaattgatt ggattatctg
     6661 ggaaagtgtt atcgatattg aacaaattag aaattcgcac atcgatctta tcacaaggag
     6721 cacaggagtt gaatctgatc atcggtgtac cgaataatca atatgaaaca gtagtgaagg
     6781 gtatctatga agggatggtg aatacggatg ctgaaagaat atcgaatggc actccatcaa
     6841 attcctgaaa tcgggtttca tgaattcaag acaaaaaaat atttatatga acaagtaaaa
     6901 aacagcgagg ccgttatcca tgaaatcggc gaaacaggac tactccttta ttttgataat
     6961 cagcaagaag aaacgattgc ttttcgaaca gatatcgatg cactgccagt caaagaagaa
     7021 acgaatctct cttttgcttc taagcatgaa ggttatatgc atgcatgtgg tcatgacgga
     7081 catatggcga tgcttttagg gttgactgac tttataacta aacacattga ggatcttatt
     7141 tataatgtag tactgatcta tcagccatca gaagaaattt ccggtggcgc acaaagcgtt
     7201 attcagtcag gattgttgga taaatatgaa gtacaagcaa tatttggctt tcatctatgg
     7261 cctggactag ctaagggaga agttttctct cgaccaggac ctttgatggc acaaagcagt
     7321 gaaacagaca ttatcattaa aggccagtct gcacatattg cttctagtga aaaaggaatc
     7381 gatagtctgg aggcagctgt acgatttttg aagaatgttt atgattttga ccggcaatta
     7441 ccggcagagt ggaatcatct aatgaaattt ggccaaatcc aaggtggaac gatccgaaat
     7501 gttttagctg gagaagttat tgtatccgga agtatccgtt cttacagttc agatatgcaa
     7561 aattatttga agaggggatt agaccattta gcagaacagt ttcactctac agctactatt
     7621 tcttttcgct ataatgacgg ctatccagct gtaatcaacc atagaggctt atatgcagca
     7681 ctgttagatt caaacaaact acatgaatta gatgctccag ttcttcaagc cgaagatttt
     7741 ggcatatata cgcaacaata tccatgtgta tttttctttt taggtgtcgg tgatacccct
     7801 gctttacatg atccgacgtt cgattttgat atggatgttt tagaaaatgg tgtagaatgg
     7861 tacaaaacga ttctttttac agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag
     7921 gagcgagtaa ataatgatga atttatccaa tcgatttagt cctaggcttt ctagaataga
     7981 agtatcaaaa atacgacaat tcgatcagca aatatcgtcg ataccagatg tgatcaaact
     8041 caccctggga gaaccggatt ttccaactcc tgaacatgtc aagcaagcag gaatagcagc
     8101 gattgaagaa gacttttctc actataccgg aatgagagga ttagaggaac tgcgggaagc
     8161 ggcttgtata tttcagcaac agcgttatgg attaacttat gatccgcaaa cggaagtttt
     8221 gacgacagtt ggtgcaacgg aagcgattgc ctctgcgttg ttatctgttt tggaagaagg
     8281 agacaaggtt cttattccag cgcctgctta ttcaggatat cagccattag tggaactagc
     8341 tggtgctgaa ttgatcccga tagatacttc tgacacaggg tttgtctgtc agccggaaca
     8401 atttgaacga gcatttgaac aatatggatc agctgtcaaa gcagtcattt taaactatcc
     8461 taacaatcct actgggacga cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt
     8521 aaaaaaatat ccggtatttg ttataagtga tgaagtatat gctgaattga cttatagtgg
     8581 aacgcatatg tccatagcta cttatcttcc agaacaaaca atcgtgataa gcgggttatc
     8641 caagtcacat gcgatgactg gttggcgagt gggctttatt tttgctcaaa aagcccttat
     8701 tgacgaacta attaaagtcc atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa
     8761 agctgcggct gaagcgctaa taaatggggt gaaagatagc gagaatatga aagagcaata
     8821 tcaaacacga agagactatc tggtccagca attggcgcct ttaggatttg aagtgaccca
     8881 gccaaacggt gcattttact tgttttgcaa actcccagct gccattcaaa ctgactcttg
     8941 gcagttttgt gtagaattag cggaacaggc aaaagttgcg tgtatcccag gaattgcatt
     9001 tggcccagaa ggagaaggtt atatccggat ttcttatgct agcagcatgg aaaaattaca
     9061 tgaggcctgc aaacggatca aagacttttt aacagaacag tgattcatag attttatcaa
     9121 aaaaacaatc gttaattttg cggtatattc tgcaaggaga cgattgtttt ttaaatttag
     9181 ttcaaatgaa cgcgtttctt ttgattcata actaatgtaa tgttaagttt aaattgttta
     9241 gaatgatttt aaaagaatat tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat
     9301 tgtaggtgca tctcatggag ggcatcaatc gatcttggaa ctattgacac gatacggtga
     9361 aaatgtagat atcacgctat ttgaagcagg agactatatt tccttcatgt catgtggtat
     9421 ggagctgtat ttagaagaca aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga
     9481 aaactttccg caatccaatg ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc
     9541 cgataaaaaa acagtaacgg ttactagcgc atcagataac gaaacaaaag aatatcctta
     9601 tgacaaattg attttgagtt ctggtgtcaa accaaacaat ctgccagtac caggagcaga
     9661 tctagaaaat gtctacttga tgcgtggata caattgggca accaaaatca aagaaaaatt
     9721 aaatgatccg gctattaaaa aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc
     9781 agcagaagca agccgaaaag ctgggaaaga agtcgtattg cttgatgtga tcgatagacc
     9841 tttaggcact tatttagatg ctgagatgac agatatttta gaacaacacc taaatgaaaa
     9901 cggtattgaa gttttgacaa atgcaaaaat cgaagctttt actggtaacg gaaaagttga
     9961 ggcaatcaaa acaagtgaaa aagaaatccc agcagatcta gtcattcaag ctgccggtgt
    10021 gaaggcaaat acagaatggt taaaagggat cgtcgactta gatgaacgtg gctggatcca
    10081 aacaaatgaa tacttgcaaa cgaatcttcc agatgtgtat gcagtaggag atgctacact
    10141 tgcttattct atcccagcac gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag
    10201 agaagcacgt tatgtagtaa aacatctttt tgaagaagta ccaaacaaac cgttcggcgg
    10261 agtcgtaggt tcctctgcat taagtgtctt tgactatcat tttgcagcaa gcggattgaa
    10321 tagctttaca gcagcaaaag caggagtaga tgtacgagtg tcttattatg aagatacgtt
    10381 acgaccaaaa tatgtaccag aagaacaagg aaatccaaaa gtgtccatcc aattattctc
    10441 ggatccaatg acccatcaat tgctaggagg agcagtatta tctacctatg atgttacagg
    10501 tcaaggaaat gtattggcac tagcgatcca aaacaaactg actttagaag aactagcaga
    10561 agctgacttc ttcttccagc caggctttga ccgacaatgg agcatgttga atctagccgc
    10621 tcaacaagcg ttaggagaag aaccatttgt agaataagaa aacgaaatat ttgaaaagag
    10681 gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa cagcttctta
    10741 tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac cgtttatttt
    10801 caaaaaaggg actgtgacac gatttgtgta ccgaccccca aaagttagac caaaaaatct
    10861 aacttttgga ggtcggtatt ttttatggtt aaatatactt ttgaacttaa attaaagatc
    10921 gttcatgatt atttggatgg caaaggcgat tggaccgtaa aaatccagat caagagattg
    10981 aagcggaaat gcttaaaatt cgggagaagc acaaggatta tggctgttta cggatgacca
    11041 atgaactaag aaaccgcggt ttctctatca ataaaaagaa agttcagcgc ttgatcaaga
    11101 agctaggaat aaaagtaacg gcatacacgc gtaaatcacg tcgctacaat tcttatcgag
    11161 gaaaagt
//
LOCUS       DAJSVD010000091        10980 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSVD010000091 DAJSVD010000000
VERSION     DAJSVD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.093"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(448..978)
                     /locus_tag="ON498_002157"
     CDS             complement(448..978)
                     /locus_tag="ON498_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR3940395.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1083..1634
                     /locus_tag="ON498_002158"
     CDS             1083..1634
                     /locus_tag="ON498_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3940396.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1787..2260)
                     /locus_tag="ON498_002159"
     CDS             complement(1787..2260)
                     /locus_tag="ON498_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3940397.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2398..3870
                     /locus_tag="ON498_002160"
     CDS             2398..3870
                     /locus_tag="ON498_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3940398.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3867..4577
                     /locus_tag="ON498_002161"
     CDS             3867..4577
                     /locus_tag="ON498_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR3940399.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4929..5213
                     /locus_tag="ON498_002162"
     CDS             4929..5213
                     /locus_tag="ON498_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR3940400.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(5494..5913)
                     /locus_tag="ON498_002163"
     CDS             complement(5494..5913)
                     /locus_tag="ON498_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR3940401.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5915..6499)
                     /locus_tag="ON498_002164"
     CDS             complement(5915..6499)
                     /locus_tag="ON498_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3940402.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(6546..8021)
                     /locus_tag="ON498_002165"
     CDS             complement(6546..8021)
                     /locus_tag="ON498_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3940403.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8202..8513
                     /locus_tag="ON498_002166"
     CDS             8202..8513
                     /locus_tag="ON498_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940404.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8628..10964)
                     /locus_tag="ON498_002167"
     CDS             complement(8628..10964)
                     /locus_tag="ON498_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3940405.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
BASE COUNT         3272 a         2059 c         2146 g         3503 t
ORIGIN      
        1 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
       61 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
      121 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
      181 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
      241 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
      301 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      361 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
      421 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
      481 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
      541 attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt
      601 tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa
      661 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
      721 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
      781 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
      841 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
      901 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
      961 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
     1021 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
     1081 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
     1141 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
     1201 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
     1261 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
     1321 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
     1381 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
     1441 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa
     1501 cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc
     1561 atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta
     1621 accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa
     1681 acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt
     1741 agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta
     1801 acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg
     1861 tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta
     1921 aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac
     1981 ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa
     2041 atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt
     2101 ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc
     2161 ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt
     2221 gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc
     2281 tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg
     2341 aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg
     2401 agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt
     2461 gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt
     2521 cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt
     2581 ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt
     2641 tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt
     2701 acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga
     2761 gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga
     2821 tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta
     2881 tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc
     2941 gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat
     3001 cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag tttactttac
     3061 ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt
     3121 gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc
     3181 ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta
     3241 acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt
     3301 ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct
     3361 atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca
     3421 gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca
     3481 gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg
     3541 ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg
     3601 gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg
     3661 ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta
     3721 gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta
     3781 gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct
     3841 gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg
     3901 cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt
     3961 tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg
     4021 gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag
     4081 gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc
     4141 gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag
     4201 tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa
     4261 acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag
     4321 tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt
     4381 tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc
     4441 aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac
     4501 aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag
     4561 aagcaaaagg cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc
     4621 atcaagcagt aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct
     4681 tagtgccgta gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt
     4741 taactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt
     4801 gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca
     4861 agacttttgt cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga
     4921 gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca
     4981 agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag
     5041 ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg
     5101 tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa
     5161 acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt
     5221 tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt
     5281 agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact
     5341 cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg
     5401 gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact
     5461 caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata
     5521 ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc
     5581 taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg
     5641 gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt
     5701 ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg
     5761 catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt
     5821 taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag
     5881 tttggttact tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat
     5941 gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt
     6001 ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc
     6061 gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa
     6121 tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc
     6181 ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat
     6241 cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat
     6301 caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg
     6361 cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta
     6421 ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc
     6481 gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc
     6541 attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa
     6601 tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg
     6661 aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc
     6721 attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact
     6781 acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct
     6841 ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt
     6901 tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca
     6961 tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga
     7021 ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg
     7081 tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca
     7141 gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt
     7201 gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca
     7261 tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat
     7321 agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct
     7381 ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt
     7441 tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct
     7501 tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag
     7561 actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa
     7621 atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg
     7681 tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga
     7741 ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt
     7801 ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct
     7861 tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat
     7921 tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt
     7981 tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt
     8041 gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa
     8101 agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta
     8161 tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt
     8221 catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa
     8281 acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat
     8341 ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga
     8401 ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt
     8461 atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa
     8521 ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac
     8581 tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc
     8641 aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc
     8701 gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca
     8761 ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca
     8821 ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg
     8881 cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt
     8941 tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc
     9001 cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc
     9061 atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa
     9121 tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata
     9181 cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca
     9241 actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat
     9301 tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat
     9361 aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca
     9421 gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt
     9481 tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa
     9541 tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta
     9601 ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca
     9661 agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa
     9721 cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta
     9781 tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga
     9841 ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt
     9901 acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg
     9961 acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca
    10021 tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt
    10081 tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg
    10141 attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt
    10201 cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag
    10261 ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca
    10321 tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc
    10381 atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt
    10441 tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt
    10501 gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc
    10561 atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct
    10621 ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca
    10681 atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca
    10741 ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga atcataatta
    10801 tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc
    10861 gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt
    10921 tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg
//
LOCUS       DAJSVD010000092        10845 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSVD010000092 DAJSVD010000000
VERSION     DAJSVD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.094"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            63..2852
                     /locus_tag="ON498_002168"
     CDS             63..2852
                     /locus_tag="ON498_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3940406.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2899..3426
                     /locus_tag="ON498_002169"
     CDS             2899..3426
                     /locus_tag="ON498_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR3940407.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3447..4637
                     /locus_tag="ON498_002170"
     CDS             3447..4637
                     /locus_tag="ON498_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3940408.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4677..5975
                     /gene="asnS"
                     /locus_tag="ON498_002171"
     CDS             4677..5975
                     /gene="asnS"
                     /locus_tag="ON498_002171"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3940409.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            6770..7444
                     /locus_tag="ON498_002172"
     CDS             6770..7444
                     /locus_tag="ON498_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3940410.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            7441..7782
                     /locus_tag="ON498_002173"
     CDS             7441..7782
                     /locus_tag="ON498_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940411.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            7812..8171
                     /locus_tag="ON498_002174"
     CDS             7812..8171
                     /locus_tag="ON498_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3940412.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            8695..10779
                     /gene="cadA"
                     /locus_tag="ON498_002175"
     CDS             8695..10779
                     /gene="cadA"
                     /locus_tag="ON498_002175"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3940413.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         3614 a         1910 c         2370 g         2951 t
ORIGIN      
        1 ttttctgcac tttgtagtat aatttcaaaa gaacattaga ggcaggagaa gaggaagata
       61 ttttgaaacc aagaacttat gcagtcgttg atatcgaaac gactgggacg aatccaaaag
      121 aagatcggat catccaattt ggctgcgtca tgattgaatc aggtcggatc acttcacgtt
      181 tttcgatcga tattcatcca gggcggaaga tttcaaaaca aatccagcat ttaacaggga
      241 tcacgaatca acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa acgatttaca
      301 atcttttggc agacaccata tttgtcgcgc ataatattta ttttgattat aattttttga
      361 accatgaact catgcgatgc ggacttccta gtttgaagat accaggcatc gatacagtag
      421 aacttgcaca aattttcttg ccgacggaac caagctttcg cttagctgat ttgtcagaaa
      481 gccttggatt tattcatgag aatcctcatc aggcagatag tgatgccgaa gtgacaggtc
      541 aacttctttt gctgatcgaa gaacggatga gaaaattgcc aatcatcacg ttagagcaga
      601 ttgcgcgatt aagtagacac acaggtatgg atacgagtcg tttcatttat catgtgattg
      661 aagaaatgaa agaaaaacca gagccacttg attcctcatt agaaatcgtc gatgggctag
      721 ctttgcagaa aaaagaagtg gaactattca catccgtcca ttacggcgag cggacatatc
      781 caagaaaaaa acaagcaaaa gaaaagatgt ttggtaaaac actgatgtat cgaaaagaac
      841 agaacagact gatgaatgcg atctacgacc atttcacaaa agatgaatca aaagatttga
      901 tgatcgaagc agctacagga atgggtaaaa caatcggtta tctgttgcca ttgagttatt
      961 tggctacacc tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg cagcagcaga
     1021 tcatagaaaa agaaattcca ttgctgaatc aattgctaga tcagcccata caacctgtaa
     1081 ttatcaaaag ttatcgccac tatatcgacc tgcaacgatt taaaggaaca cttgtggaac
     1141 caccagaaca aaaacaatat gctttgtatc aaatggcggt cttagtttgg ctgacgcaaa
     1201 cgaagaccgg tgatttggat gaattacatt taacaaactt gaaccatgca ttctttgcag
     1261 atatcgcgca cagaggtact gggtttttag caagaaatca gtcgttttac gaacaagact
     1321 ttgttcgttt cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc aatcatgcgt
     1381 ttttagtaca agaaacacag cgaaaagaac cattgcttcc agctagtcca tttttgttga
     1441 tcgatgaagt acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa cgattaagta
     1501 ttaactattt ccataaacaa atccagcaat tagaagaacc aaatcagctg tttgaccgaa
     1561 tacaaagcta tttaccgaaa gaacatgaag ccttacgctg gttatcgatt tatcgagaag
     1621 aactttctgc gctatgcgaa gcacagattt gtatggcaga aggattaatg agtttagtga
     1681 gcggcaagca acccaacgga cagtatccag aagaagtcat gatgacaaaa gagatgctgg
     1741 atcagctgcc actggaatct gggacagcca ttgatcacct gcagcttttt tatcaggaga
     1801 ttcagcagct acagtcacaa atgcgtctga cttttgagat tatccaagaa agctggacaa
     1861 accagatgcg gatcgattta gcaagtttat atagcttatt tgatcaatta gacaagcagt
     1921 acttgctgat ggatcaatgg ctggaagact ggcgagatca tttgatccat tggttcgttc
     1981 ctgggaacac agcgcagcag atgatttttc atctccatga tttccaagcg gctatgttgc
     2041 cgtcgactgt ttggtatcct cgatatgatc gtattcttta tattggagga acgctgaaga
     2101 tcggccctaa tcgacactat ctctctaaga ggcttggcat tgaagcagca cctcttaaag
     2161 tgattgcctc tccttatgat tatgagaaac aggcaaggat ctatgtgcca aaagaggcac
     2221 ctgccgtcca tcaagtcagc aatcaagcat acatcgatta tttagagaca gcacttacag
     2281 gtctgatcga acaagaagat cggccaatac ttgtgttatt cacttctcat gatgttttgc
     2341 agaaggtcta tcagaaaatg catctgtcta tgctagaaaa aggacgagaa atactagctc
     2401 aagggtttgg tggaagccgg gagaagcttt tgaaacgctt cattttgtca gaaaaaagcg
     2461 tattgtttgg cgctgacagc ttttgggaag gtgtagattt gcctggagat gctttgcaga
     2521 tcgttgtagt cacacgttta cccttcgata atccgcttcg cccgatggtc aaagctagga
     2581 acgcttattt agaatcagag gggatcaatc ctttttacca ggaatccgta cctaaagcag
     2641 ctttgaagtt acgccaagca ttaggtcgct tgataaggac ggagacagat cgtggcgtat
     2701 tgcttatttt ggatcgaaga ctagtcactg caaaatatgg cgcacggatc atccatgcat
     2761 taccgaaaaa agtaccgatc aaagagctag ctatggagga aatctatcag gatatccatg
     2821 aattcttaaa gaaaaatgaa gaaggaagtt agaggagctg tcttttttca taattaaaat
     2881 ctgctataat gaaaaggaat gaagataaag gagcgacgac ccttgaagaa aaaacagaca
     2941 aaggaaaaaa cgattaatcg cgtgttactt ggcttgatcg tatttttatt agcggtcatc
     3001 gtgatcagct cgatttttta cttgcgttcc agtcgtccgg cggtgcaggc gaagaaagaa
     3061 gcagtagaaa tcgcaaagaa atatgcaaat cttgaggaaa ccgatcattt ttattggttt
     3121 actcgaaaga agacatactt ttcactgaca ggtacaaatg ataaaggaaa agaaatcgcc
     3181 gttatcattc ctaaatctgg tgaaaaagta aaagtattgg atcaaacaaa gggactgact
     3241 gaaaacgaag caaaacagac aattgctgcg gcccatcctg aaatccaaat cgaaaaagct
     3301 gctttaggga tgtatgatga tcagcctgtg tgggaagtag taggaaaaga aacaaatggg
     3361 aaactgaatt attacctgat tggctttgaa tctggagatg aagtcaagac aatcaaggga
     3421 atttaaaagg taaaggaatg aatgggatgg agttatcaaa aagaacaaaa cgattagaac
     3481 cgtctgtcac tttggcagct gcggcaaagg ccaaggcatt aaaagcaaaa ggcagagatg
     3541 tgctgagttt gactgtggga gaaccggatt ttgcgacacc agaaaacatc caagaagcag
     3601 caatcgaagc aatccgcaat gggaaagcta gctattatac accaacagca ggaatcccag
     3661 agcttcgaca ggcaatcgtt gattatctga agaaaaacta tggacttgtc tatgaacctt
     3721 cacaaacagt cgtaacagac ggagcgaaat ttgctttgta tacccttttc caaacgatcc
     3781 ttgacccgca agatgaagtc atcattcctg tgccttactg ggtcagttac ggtgagcaag
     3841 taaaactggc agaaggactt cctgtctttg tgaaagggga agaatccaat agctggaaag
     3901 ttactgttga acagctagaa caagcacgtt cagaaaaaac aaaagccgta atcatcaatt
     3961 ctccatcgaa tccgactgga atgatttata gcaagaatga acttcaggca atcggtgaat
     4021 gggcagtaaa aaatgatatt ttgatagttg ctgatgatat ttatggtcgt ttagtttata
     4081 atggcaatga gtttactcca attgcaacga tttcagaagc aatcaaaaat cagaccatca
     4141 tcatcaatgg cgtgtctaaa acatatgcga tgacaggctg gcgcattggt tatgcggtgg
     4201 ggaattctga gatcatcaat ggaatgattg ctgttgcttc ccagtcaacc agtaatccga
     4261 cagcagtcag tcaatatgca gctgttgaag cattgaaagg tgagcaagat acagtagaag
     4321 aaatgcggaa cgcctttgaa gaacgcctga atacgttata tccgcttgtg gcagaactgc
     4381 caggtgtttc tcttgaaaag ccgcaaggag ctttctatct ttttcctaat gtaaaagaaa
     4441 cgctacgcat gtgtaaatat gaaaatgtca cacaatgggt agaagatctt ttagaagaaa
     4501 caggggtagc gcttgtgact ggcgaaggct ttggggcacc agaaaatgtc cgcatgagtt
     4561 atgctactga tcgcgctacg ttagaagaag cggtacgcag aatcaagcaa tttattgaga
     4621 gtaaaagtca aaattaatcc tgaagaaatt caaggaatat aaaaggagag tcatacgtgg
     4681 aacaaattca aatcatcgat tcgaaaaacc atgtgggaga aacagtaaca atcggcgctt
     4741 gggtcgcaaa taagcgatca agcggaaaaa tcacattttt acaattaaga gacggaacag
     4801 cctttttcca aggtgtagtt gtaaaaagcg aagtgtcgga agaagttttc caaacagcta
     4861 aaaatctaca ccaagaaaca tccgtgttag taactggaga aatccgtgaa gacagtcgtt
     4921 caaaatttgg ttatgaaatc ggaattacaa atgtggaagt ggtaagcgag agccacgatt
     4981 atccgatcac accaaaagaa cacggaacag acttcttgat ggatcatcgt catttatggc
     5041 tgcgttcttc aagacaacat gcgattatgc agatcagaaa tgaaatcatt cgtgcaacgt
     5101 atgaattctt taatcaacat cattttgtca aaattgatcc gccgatcttg actggttctg
     5161 caccagaagg cactacggat ctattcgaaa caaattactt tgatcaaaaa gcttatttat
     5221 ctcaaagtgg ccaattatac atggaagctg ctgctatggc tttcggaaaa gtcttttcat
     5281 tcggtccgac tttccgtgct gaaaaatcaa aaacacgacg tcatttgatc gaattctgga
     5341 tgatcgaacc agaaatggct ttcactcatc aagaagaaag tttagaagta caggaacaat
     5401 atgtagcatt cttagtacaa aatgttttag atcattgcga atacgcatta gatgtactag
     5461 gacgagacaa agaagtctta aaaacttata caaaattgcc attccctcgt attacttacg
     5521 atgatgcaat cgaattactg aagaaaaatg gatttgatga catcgaatgg ggcgaagact
     5581 ttggttcacc tcatgaaacg ttcattgcga attcattcga acagcctgtc tttatcctaa
     5641 actatccaaa agccatcaag ccattctaca tgaaaccaca tccaacaaga gaagacgtgg
     5701 taatttgtgc agatatgatc gcaccagaag gttacggtga aattattggc ggaagcgaac
     5761 gggcaacgga ctatgatttc ttgctagaac aaatccgtaa tcacggtttg gatgaaaaag
     5821 aatattcatg gtatcttgat ctacgtaaat acggttctgt tccgcactct ggttttggtc
     5881 taggtcttga acgaacagtc acttggattg ctggtatcga ccatgtacgt gaagctattc
     5941 cattcccacg tttgttgaac cgaatttatc cataagaggt tgtgcgcctg ccgttcagct
     6001 ccgagtcaca aggagtaatt tcaaaaaaca gtttttcatg tttgatgact attgtgactt
     6061 gtggccggag agcaacgttt atttaaaaaa aagaactgcg aactctcgca gttctttttt
     6121 ttaaatacgg gataatggaa gtttccagca gacaagagaa gaaagcaaaa gaaattgcta
     6181 tttgattcac aaggttctat aacgaaatcg caatcaatca aacatttaca aagaaggtaa
     6241 ttcaaatgga aatctgattt gatgatgctt ctgctgttga aataaagcag catctagcac
     6301 ctgaaaacaa actaatagta acattcgaag atggagtagg tccgtatttt cagcatgcaa
     6361 taatccatat gtaagttcaa ttcacccttg aaatcatcgg accagataaa gttatgacca
     6421 atctatccca agcaatctag aagagatatt agtaaaggga catttactag aagtcttaga
     6481 tgagcctata aatatatgtt tcgatggcgt catcagtggt ctgattgacg gaagcttggg
     6541 tgtgatcgat ctctctggac caaacaacga aggaatcaag gggaatccgg cacgttaaga
     6601 aaaaaatagc ataaaaaact agacacttga tgagcgttca ttcaattgtt tgaaggaact
     6661 aggctggtgt cattttttat acatatattt tctctttcgt cttgataggg cttatgggat
     6721 aatataagga agaaaatcat cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg
     6781 tatattattt atcggtgata gcattacaga cgcaggaaga taccgagcag aagcaactga
     6841 tctaggtaaa ggttatccac ttttgattgc cggaaaacta atctcccaat atccaacgat
     6901 ggaatttgaa tgcatgaacc gtgggatagg aggagacaaa attgcagatc tgcaagtacg
     6961 ctggcaagaa gattgtttag ctttacaacc tgactatgta tcgattttga tcggaatcaa
     7021 tgatacctgg cataatacta gcgatcaggc tatattcgga actgagaaat ctgctcagca
     7081 atttgaatcc gtttatcgaa aattactaaa tgaacttcaa acagcggaaa tctgcaacat
     7141 catcttgatg gagccgtttg tacttcctat aaccgacgac cgtcatggct ggcgtaaaga
     7201 cttagaccct aaaatccaaa ttgttcgtaa acttgctaag gaatatggcg cattgctggt
     7261 tcctttagat ggtatattca atgctttagg gattgcttat ggtttttcga aatatacaag
     7321 agaagatggg gtccacccaa cgattggcgg acatgagatc attgcacaaa gctggataga
     7381 tgcaattgtc gcagtcgatt tttttagtgg agataaaaat gagcgaaaga aaagtgaaaa
     7441 atgatggaaa taaggtgggc agtaaaagaa gatttacctc aaatcaatca gctgattact
     7501 gatcatccag aatcattcgt aaaagcaaaa acggcagctg acctaaatga taagaataaa
     7561 ctattgctcg ttgctgtaac aaaagaaaaa cggattatcg gatttgcgca agcggacgtg
     7621 caacagtcag aagaacgatt ggatcagcga ccatcaggaa aactaatcca acacacatta
     7681 gtaacaaaag aaaatacgga agaaatccaa aaaaaatttg cagaatactt gatgctggaa
     7741 gctaaaaaaa gaagaataaa atcaattttt gcccagtttt aatcaaaaac agtaatagaa
     7801 ggagaaatac aatgagttgt ttaggaaatg tcatctggtt tatattcggc ggactaatcg
     7861 gtggtcttag ctggttagca gcggggatac tttggtgtat cacgatcata ggtatcccaa
     7921 tcggtatgca atgttttaag atcgcctccc tttcttttgc gccttttggc aaagaagtaa
     7981 tttatcacca gtctggcatg tcattgatcg ttaatatcat ctggctgatc gtttcaggcc
     8041 taccaattgc tctcgcacat gtcgtcagcg gcatccttct atgtatcacg atcataggga
     8101 taccttttgc caagcaatct ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag
     8161 aaattgtata aaagagaata ataaaggtta gataaagcat agagccaggt gggatacatg
     8221 tatttcacct gactctatgc gttttcctgt ataagttctt ctatgtgact tttacgtctt
     8281 ttatttatat ggggttactg tttatggaaa gttgaaataa ttttcattaa agaactcttt
     8341 ctatattttt gaaagcgtag tggagcttcg tatatctggg aaggacattt tgcagggatt
     8401 gaaaaaagat attgtcctat cgaaaaatta atgaatttag acaaattgcc agcaacaggc
     8461 ttcaaactta gttgctatcc tgtcaagatc aagaaggcaa gtgcgggttg gataagagca
     8521 gtggcaatga tcgaagagaa aaagaaagaa taagccaaaa catctgtatg gaagaaattt
     8581 tccgatgcag atgtttttca ttttttcttt cggatacctt gacatatgaa taaccattca
     8641 tttataatta gttatgaaga gatattcata tgttgaggcg aggaggtcat taaaatgaaa
     8701 agttacagat tagaaggttt agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt
     8761 cagaaaatca atggagtcaa ggaagcaacg gtaaacttca cttctggaaa actaacgatt
     8821 gacgcagagg aagatcattt ggctacgatt gaacaagaaa caaaaaaagt agtgaaggaa
     8881 ttagaaccag atgtcaaagt aacagaaata gataaggaaa aagtatctga acatggaaat
     8941 gaaaaaaaga gaaatacgct gttccggatt ttattttccc ttgcaggtat cgctttattg
     9001 ctattatttg attttaatga accaattcgc ttgatagggt atttattgat ttatctgctg
     9061 ataggatatg atgtagtgaa gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat
     9121 gagaattttc ttatgtctgt tgctacgatt ggcgctatgt tgatcggtga atatccagaa
     9181 gccgtagcag tgatgctatt ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt
     9241 cattcaagaa aatcgattcg cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag
     9301 acggcggaag gattgatgac cgtgaaccct gaagacgtgc tgatcggtca gtttgtactt
     9361 gtgaagcctg gtgaacgtgt accgttagat ggaacgatca tcgaaggaga atcattagtc
     9421 gatacgtcag cacttacagg agaatcagtc cctaaatctg tatatgttgg tgaaacagta
     9481 ttaagcggat ttatcaataa aaataaacca tttttagttc aagtagaaaa atcttatgaa
     9541 aattctacta tcagcaaatt attagaatta gtagaaaatg ccagcagtaa aaaagcacca
     9601 gctgaaaatt tcattactaa attcgcgcgc tattacacac cagtcgttgt aggattggcc
     9661 gttttattag ctgttttacc tccacttgtt gtttctagag cggcgttttc tgaatggatc
     9721 tatcgagcat tgacattttt agtgatctca tgtccatgtg cattagtgat ttccgtgcca
     9781 cttagtttct ttggcggtat cggaggagca agtaaaatcg gtgtattagt aaaaggttca
     9841 aattacttgg agttgcttgc acaaactgag acagtggtat tcgataaaac tggcacatta
     9901 acgaaaggtg acttttcgat ccagacgatc gatccaacag gaattgatcc aaagaaattt
     9961 atgcaatatg ttgcaagtgc agaacagttt tctacacatc cgattgctca atctgtttta
    10021 gaaggctacg caggtccgct tttaccaaca gctaatatcc aggaatttgc aggtgaaggg
    10081 atcttagctg aagttgatgg caaacaagta ttagtaggta atcacaaatt gatggaaaga
    10141 tttgaaatct cttttccttc aagtcaagaa atcggtacat tgctttatct agcgatcgat
    10201 caaagttaca gtggttatct tgttattgca gataccttga aagaagatgc cgtagacgca
    10261 ttggttcaat taaaacaggc tggtgtgaaa aacacagtca tgttgacggg cgactcgaaa
    10321 aaaattgctg atcatatcgg gaagcaagta ggcgtggaca agatatatag cgaattgctt
    10381 ccagaagata aagtgcagcg gttagaggaa atcttacaaa gtaacaataa aaagaccgca
    10441 tttgtcggag atggaatcaa cgatgcaccg gtgctagcaa gagcagatgt aggaatcgca
    10501 atgggtggat tgggaagcga tgctgcaatt gaagcagcag atgtagtcat catgaatgat
    10561 caaccatcga agattgcaga agcaatccat ttagcaaaga aaacattgaa aatcgtcaaa
    10621 cagaacattg tctttgctat tgggataaaa attttggtac tttccttagg tgcttttggt
    10681 tttgcatcaa tgggagatgc agtatttgca gatgttgggg tgacagtatt agctgttttg
    10741 aatgccatgc gaagcttgca tgtaaaaaat aaacgttgat caatgaagag gacgaatgaa
    10801 cgtcctcttt tttttatatc cttttcttaa tgaaaaatgt aaaga
//
LOCUS       DAJSVD010000093        10825 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSVD010000093 DAJSVD010000000
VERSION     DAJSVD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.095"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..652)
                     /locus_tag="ON498_002176"
     CDS             complement(<1..652)
                     /locus_tag="ON498_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCR3940414.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLK"
     gene            1298..4105
                     /locus_tag="ON498_002177"
     CDS             1298..4105
                     /locus_tag="ON498_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3940415.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4296..4787
                     /locus_tag="ON498_002178"
     CDS             4296..4787
                     /locus_tag="ON498_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940416.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4890..5879
                     /gene="manX"
                     /locus_tag="ON498_002179"
     CDS             4890..5879
                     /gene="manX"
                     /locus_tag="ON498_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3940417.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5912..6715
                     /locus_tag="ON498_002180"
     CDS             5912..6715
                     /locus_tag="ON498_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR3940418.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            6736..7650
                     /locus_tag="ON498_002181"
     CDS             6736..7650
                     /locus_tag="ON498_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR3940419.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            7733..8107
                     /locus_tag="ON498_002182"
     CDS             7733..8107
                     /locus_tag="ON498_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR3940420.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8246..8491
                     /locus_tag="ON498_002183"
     CDS             8246..8491
                     /locus_tag="ON498_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3940421.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8663..9727
                     /locus_tag="ON498_002184"
     CDS             8663..9727
                     /locus_tag="ON498_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3940422.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9973..10398
                     /locus_tag="ON498_002185"
     CDS             9973..10398
                     /locus_tag="ON498_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3940423.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10391..>10825
                     /gene="nusB"
                     /locus_tag="ON498_002186"
     CDS             10391..>10825
                     /gene="nusB"
                     /locus_tag="ON498_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3940424.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT         3520 a         1774 c         2310 g         3221 t
ORIGIN      
        1 tttttaaatt ataaataatt tcgtttaaag aatcacttat cctatcaaca gtatctttat
       61 caactgcaac attcaatact ttaccaagag tatcaatagt taatgtaccc aacagcactc
      121 ctcttttttt actaatatta tttaaattgt ctaatataat actttgtaca ttattgccta
      181 tcattttttg ttgagctaca attaaacgtt tattagttaa tgcgtatgca taattattgt
      241 catgcttagt tgatgagata taattatgca atcctacaaa gcagaagatt acgtattcgt
      301 cactgttaag ttgatctgat attaagttaa aatgatgagt tgtccatttt ttagtcattc
      361 ctttaccgta gccgtattta agacaaaatt ctaccatctc ttcagtggtt ctcattctat
      421 cattacctat atcatgattg ttttgtgcag tatgatcaga tatgtttaat tttctttttt
      481 ctgatggatc cttatctaaa tcttctaaat tcaaagatct tattatttta taaggagtag
      541 tcataccgta accagctttt ttcatacaat tgccgcaaac aatttgttta tctttgattt
      601 tatatgaagt taagccaatt ttacctccgc actttgcaca ttgtttactc acattagttc
      661 ctcctagata tcagctttct actactatta acattctatc aatagtgacc atactttgac
      721 catacttttt agaaagaata gaaaattcca agatatttaa taatagaaga aaagcactaa
      781 catcatgggt tcagaagaat tgagagctta tcaggaaaca gtataattga agtgtctgtt
      841 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
      901 ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac gaagtttact
      961 ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt tgataactat
     1021 cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat atggtaaaca
     1081 gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt cttttaaatt
     1141 gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa
     1201 acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct atagaaaagg
     1261 agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc
     1321 tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg ctgaagtgtt
     1381 agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact
     1441 gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta cgcacgtaaa
     1501 gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag aaaaaaatac
     1561 actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg gcagcatgaa
     1621 aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat taaattgttt
     1681 gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt atcattttgc
     1741 aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt gtgctgatta
     1801 tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag gtgcttttac
     1861 aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta tgctgttttt
     1921 ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact ttatggatca
     1981 tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg tacggatcgt
     2041 aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc ggcgtatccc
     2101 aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag tcgatttagt
     2161 aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt tgacagaaga
     2221 tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa
     2281 tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg aaatctcgat
     2341 cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag caagcaaccg
     2401 cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag caccaaatga
     2461 tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat atgatatcat
     2521 cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa tcaatcattt
     2581 catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg gtttttcttt
     2641 taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag tgaccaatca
     2701 gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact ttatttatgc
     2761 tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag aacggcatac
     2821 gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg ttgcgaaaga
     2881 agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg aagactatta
     2941 cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa tcggtgtagt
     3001 cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc aacagttgtt
     3061 agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga aaacagcatt
     3121 ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca tactcttagt
     3181 agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg gaattccagt
     3241 acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa aggtctcagt
     3301 attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag gttacgcaga
     3361 aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa aagctgtttt
     3421 ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga tcgaacgagc
     3481 ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg tggaagtgaa
     3541 agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg ggattactga
     3601 tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa atatcgaagt
     3661 cattttggat cagttgctga tggaatcaga attggaggaa actgaattca tctccctaga
     3721 tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca tctttattaa
     3781 tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc agacgacggg
     3841 aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag caggaatgat
     3901 cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga caaatacaac
     3961 gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag atcagataaa
     4021 agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata atcagttaga
     4081 taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat agtgtatcgt
     4141 ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa aagttggcac
     4201 gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa tgataggggt
     4261 ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg gcacgtatag
     4321 atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac
     4381 gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt ctactaaaag
     4441 aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg attcaaatct
     4501 atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat ccccaagatg
     4561 ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt
     4621 ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa gatattgatt
     4681 cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta cctgctgaca
     4741 ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt
     4801 tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt ttgaatttgt
     4861 tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca agtcatggac
     4921 aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa gaaaacgtca
     4981 aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa ttggaagaag
     5041 cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta tggggcggaa
     5101 caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg gcaatcgtca
     5161 gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca atggaatctg
     5221 ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg
     5281 aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa cctaaaggag
     5341 cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg
     5401 actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg caaccaaacc
     5461 gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac
     5521 aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg atcgaagtag
     5581 caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat cctgaagacg
     5641 tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc
     5701 attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa gacgttgaag
     5761 cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta cctaatgatt
     5821 cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca aaagcgtaat
     5881 aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa ttttagtcgt
     5941 attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat ttcatcaacc
     6001 actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg gtatcgtact
     6061 tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc
     6121 agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg gacaaggtgt
     6181 taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag ggttgttctt
     6241 aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg cggctgctga
     6301 agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt
     6361 tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg tgcaatcatt
     6421 ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg gaatggtcgt
     6481 agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat ggccattctt
     6541 cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc taggtgcttt
     6601 aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt catcaaataa
     6661 tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact attaatcttg
     6721 aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga tcgtttagct
     6781 gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat gcaaaacggt
     6841 ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa agaagaacgt
     6901 tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat tgcttctcca
     6961 attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat
     7021 gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt tggtgaccca
     7081 gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt agctatggga
     7141 ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg ttgggcattc
     7201 atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga tgacctttct
     7261 ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt cgtattagct
     7321 gcccttgtag aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta
     7381 gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg aatcaagagt
     7441 gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac aacattacaa
     7501 gacaacttag accaattgat tcctggttta gctgcattag gattaacatt gttatgtatg
     7561 tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt cgtagtcggt
     7621 gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg gcgttgtccc
     7681 gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga aaatggttca
     7741 atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg gtctgacaga
     7801 ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc gagatagacg
     7861 caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag tcatgctaaa
     7921 gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat acacattttc
     7981 ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg aagatagaat
     8041 ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg
     8101 caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt actttgcaga
     8161 agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata tgctaaaatt
     8221 gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg ttggagccgt
     8281 gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa
     8341 tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg aatggggacc
     8401 aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt tggtagcagt
     8461 tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa ctaaaaggag
     8521 ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa gaagttttcc
     8581 ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg
     8641 caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca
     8701 agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt acttaacgaa
     8761 ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt ttattactga
     8821 ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt
     8881 cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc actcattagc
     8941 ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa tcatcgatgc
     9001 ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag atgaagaaga
     9061 aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc acatcctgaa
     9121 aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact tttatatgcg
     9181 ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat tgcgttcggc
     9241 tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga tcacattgga
     9301 ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg ctattggtga
     9361 tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat tagcagttat
     9421 tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta gagattatat
     9481 ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga tcggtttaga
     9541 gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac caggaaatgt
     9601 gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta ttgaggatga
     9661 cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat
     9721 cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag actttgttat
     9781 agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta agagataata
     9841 aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca gttttcttac
     9901 cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg
     9961 aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca agatttagga
    10021 gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc ttctaaagta
    10081 gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt attaggacgt
    10141 gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa agtagatctt
    10201 tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat gcaggaacgg
    10261 gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa tatccatgta
    10321 gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt tgaagacgaa
    10381 gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg cattgcaggc
    10441 tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt tcaacgcaat
    10501 cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc ctgtttatct
    10561 tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa
    10621 acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg tgatcttgcg
    10681 tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg ctttaaatga
    10741 agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg tcaacggtgt
    10801 tttatccaat gtattgaaag aatta
//
LOCUS       DAJSVD010000094        10290 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSVD010000094 DAJSVD010000000
VERSION     DAJSVD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.096"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            169..318
                     /locus_tag="ON498_002187"
     CDS             169..318
                     /locus_tag="ON498_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR3940425.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            329..1843
                     /gene="dltA"
                     /locus_tag="ON498_002188"
     CDS             329..1843
                     /gene="dltA"
                     /locus_tag="ON498_002188"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR3940426.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1840..3045
                     /gene="dltB"
                     /locus_tag="ON498_002189"
     CDS             1840..3045
                     /gene="dltB"
                     /locus_tag="ON498_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR3940427.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3090..3323
                     /gene="dltC"
                     /locus_tag="ON498_002190"
     CDS             3090..3323
                     /gene="dltC"
                     /locus_tag="ON498_002190"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR3940428.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3326..4594
                     /gene="dltD"
                     /locus_tag="ON498_002191"
     CDS             3326..4594
                     /gene="dltD"
                     /locus_tag="ON498_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR3940429.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4735..5817
                     /locus_tag="ON498_002192"
     CDS             4735..5817
                     /locus_tag="ON498_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR3940430.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5817..6620
                     /locus_tag="ON498_002193"
     CDS             5817..6620
                     /locus_tag="ON498_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3940431.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6690..7430
                     /locus_tag="ON498_002194"
     CDS             6690..7430
                     /locus_tag="ON498_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3940432.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7441..8445
                     /locus_tag="ON498_002195"
     CDS             7441..8445
                     /locus_tag="ON498_002195"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR3940433.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8490..10253)
                     /locus_tag="ON498_002196"
     CDS             complement(8490..10253)
                     /locus_tag="ON498_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940434.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3310 a         1702 c         2085 g         3193 t
ORIGIN      
        1 aaaagtacga gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc
       61 tatgtgacaa aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt
      121 gtcaaaatta gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg
      181 cgttctaatc caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt
      241 gcagtactgc tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt
      301 atctataacg aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga
      361 tgattgggga ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt
      421 tggtgaattg aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg
      481 cgatggacca attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg
      541 ggttgtcaaa tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat
      601 cctatcgatc tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga
      661 agatattcct tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc
      721 agtaggattt tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac
      781 ttctggtact actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt
      841 tgttcaatgg acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc
      901 gccattttct tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat
      961 gctgaagccg ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc
     1021 agccttgaag ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga
     1081 acctacattc aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga
     1141 attgacgaaa aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa
     1201 tacttacggt ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt
     1261 actagatagt tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat
     1321 tgtccaagaa ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc
     1381 aagcgtatca aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca
     1441 aaatgactta gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt
     1501 attttatgaa ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg
     1561 ggatatcgaa cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa
     1621 ataccaagga acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa
     1681 acagccggat tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga
     1741 ttatatgatt cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa
     1801 aatcgatcga aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc
     1861 ccttatgcag agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta
     1921 tctttattga tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt
     1981 ttatttgtca gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt
     2041 ttgtggcaga cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca
     2101 ggatggtttt ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact
     2161 cctttttttg aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc
     2221 tttaaatctg tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca
     2281 tttcgttata tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc
     2341 tataggagat ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt
     2401 ttgggaaaag gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat
     2461 tattttggtc aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt
     2521 tcatgggcat tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct
     2581 ggctatagct tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat
     2641 ttcaataaac cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg
     2701 ctgtcctttt ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa
     2761 aaatttttta aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta
     2821 atgggtatat ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt
     2881 ctgatctgtc tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct
     2941 tctaacaaat ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc
     3001 ttcctgattt tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat
     3061 taatttatat aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag
     3121 aaataacagg aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt
     3181 tgcttgattc tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc
     3241 aagtccctgt atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac
     3301 aagcaactgc attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt
     3361 tgttttagct gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc
     3421 taatccagaa acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga
     3481 tgcgataaaa aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga
     3541 attaagcaga atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta
     3601 tcgtccattt ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg
     3661 ttctgcagga gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt
     3721 cgttaaaaat ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata
     3781 cgactggttg ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt
     3841 attaactttc tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa
     3901 aaaagggaag ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct
     3961 gaagagagag gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga
     4021 tcatgagtca aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa
     4081 tcaaattgga gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac
     4141 gcatcgtatc cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg
     4201 tttttcgcca gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa
     4261 cgaagtattg ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc
     4321 tcaagaaatg ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt
     4381 caatcgaata gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat
     4441 ccatctaggc tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga
     4501 aaatcatatt actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca
     4561 acatcaaata ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt
     4621 gttttcttta tttttgaata ttctgaataa aagataaaaa taccaagttt gcctgaacaa
     4681 aaatcataag caatggtata atcacaatat gaatatagaa atgggggaaa acatttggat
     4741 acaaaagaag aacagctact tattgattta acttctgcaa aaggagtacc tggaaatgaa
     4801 gaagaggtaa gagaagtatt cagagaatat gccaaaccat tcgctgatga tattttttat
     4861 gacggtttag gcagtgtgat tgccaaacat ggtgctggcg gaggccccaa ggtctttatt
     4921 tctggacata tggacgaagt tggttttatg gtaacaaaaa taaccgaaaa aggattcttg
     4981 gaattccaaa ctcttggagg ttggtggggg caagtcatgc ttgctcagca agttgaaatc
     5041 aaaacacgtg aagggaaaat cgtccacggt gtgattggtt ccaaaccacc acatgtttta
     5101 acacctcaag taagaaataa accttatgaa ataaaggata tgttcattga tattggcgca
     5161 tcaagccaag aagaagccaa agagtggggc atacgtcctg gagatatggt cactccatat
     5221 attgaataca aacgaatgaa tggcagtaaa tatttactgg caaaagcatg ggataatcgt
     5281 atcgggacag ctgtttctct gcgagtatta gagaatctat ctaaagaagc acacccaaat
     5341 gttttattcg caggaagcga tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt
     5401 acacaccttg taaatcccga tattgctttt gctttagata ctggtacagc aggagacact
     5461 ccaggaatga caccaaaaga agcagattcg attttaggaa aaggcccaca gattttgatt
     5521 tttgatgcat ctatgatccc gcataaaaaa ttattgaatt ttgttattga tgtagcagag
     5581 gaaatggata ttccattcca atatacagtg atttctggcg gaggaactga tgcaggacaa
     5641 atgcatttga caagagatgg tgttccgtct gtagccatta ccgttcctgt tcgttatctg
     5701 cattcccata catcagtgat tcatgaagat gattatttga atacagtgaa actagtcaca
     5761 gaattagtga aacggcttga tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga
     5821 ataatagaat acctatttct gaatatcgga aaaaagcacg agtttctttg gaaaatcaat
     5881 ggggaatcaa tacttggctt gttttcttat ctataatatt tacgagcgtc atccagaact
     5941 tttttgacgg tttcagtcct tttccggaag aaagcatgca gcagcactta ttgagttttc
     6001 tagtcgataa tttactttta ttcgcattca actatgcatt gttttacatt gcgctagtag
     6061 ttgtacgggg tggcagacca aaaagtaacc ttttattttc agttttccaa agaaagtatt
     6121 ttggttcgat acttggcatc aatttgatca attcagtcat caactggcta ctcaacagtt
     6181 taatctttct gcccatgttt ttaatcagcg gagtgagtac ttatatgcag ctgcttttca
     6241 gttcaaatgc ggtacctacg ggtgtcatgc cagaagattt tcttgatatc agctttgttt
     6301 tatcgatgat agggatgatt tttgtttcag cactcatcat gagtattgtt ggcggtttat
     6361 tccaatttgc tgcatggaca aagttcgact atcctgattt gtcggttatt caatgtttga
     6421 aatatggctg gtttttattg aaagatagaa ttggtacgta tattttgttg cagctgtctt
     6481 ttatcggctg gtatttgcta ggagcaatcg ctttgttcat cggtcttttg tgggtcattg
     6541 tttatgtgaa tgtgtcagtt gcagaatttt atgaacaagc acggattgaa aaaggaggtc
     6601 cagacgccta ttttacttag catgtaagca gtttattcat caaaaaaata acttttgtgg
     6661 taggataaat ctttgagggg gaatgtacga tgaaattaac tgttttagga tgcttaggag
     6721 cgtatcctta taaaaatcaa gggacaacag gttatttgct tcaatctgac gggttcaatt
     6781 tgttgatcga tgcaggtagt gctacgctga tcaaattaca agaacacctt gatccacttg
     6841 atctagatgc tgtgatcctt agccattatc atcatgatca tattgctgac cttggtgtat
     6901 tacagtatta ctggcaactt catcctaaga gaaatccgga gacattgctt tcaatttatg
     6961 gacacacaaa agattccatt cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac
     7021 atccttattt tgaagctgaa gaattaaaaa ttggtccttt tctgattact tttatggaaa
     7081 caatccatcc agtagtatgt tacgccatgc gtatcgtaga agaaacaacg gggaaagtat
     7141 tcgtatttac aggtgattca ggttatttgg aaagtttcgc tgattttgcg aaagatgctg
     7201 atttgtttct agcagatacc tatctttttg aaggaaatga acatcatcat gcccatttca
     7261 cttcaaaaga agctggagaa cttgcgaaat cagctaacgt aaagaaactt gtacttacac
     7321 acctacctca gtttggtgat ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca
     7381 aagggattga tatagaactt gctgcagttg acaaagtgtt taacatttag gaggaagaac
     7441 atgatttttg taccaaatga aaaccacgac ccaagaatca atttagcaat tgaacaatat
     7501 ttgctgcaag aaatgccttt agatgaacct attttgctct tttatatcaa tgaaccttca
     7561 atcatcatcg gcagaaacca aaatacgctt gaagaaatca atcgagatta tgtggaagaa
     7621 aatggcattc atgttgtccg tcgcttaagt ggaggcggag ctgtctacca tgattttgga
     7681 aacctgaact ttagttttat catgcctgat gatggggatt cattccgtga ctttgcgaaa
     7741 gtaacacagc caatcatcca agcacttcat gaattgggtg ttgctggggc ggaattgaaa
     7801 ggccgaaatg atctagtcat tgatggcatg aaattttctg gcaacgcgat gtatgctacc
     7861 aacggtcgga tgtttgctca tggaacaatt atgttcgact ctgatatcaa tgaagtagtc
     7921 aatgcattaa aagtaaaaaa agacaaaatc gaatcaaaag gaatcaaatc gatccgttct
     7981 cgtgtaacga atatcaaacc atttcttcca gaagaaaagc aaaaaatgac aacagaagaa
     8041 tttcgtcaag aaatcttgtt gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg
     8101 tacgaattga ctgatgaaga ttggaaaaaa atcaatcgaa tctctgagga atattatcga
     8161 aattgggatt ggaattacgg gaagtctcca gatttcaatt tctctcgcca aaaacgtttt
     8221 tctatcggtt cgattgaagt ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa
     8281 atctttggcg atttcttcgg actaggagat atctctgacg tggaaaaaaa attggttgga
     8341 cagaaatatg ataaagcatc tctatcaaaa atagtcagcc aaattgatgt gaagaaatat
     8401 tttggtgcga tagaaccaga agaattgctt accttacttt attgaaaata aaaaatagcg
     8461 gaatccacat atatggattc cgctattttt tatacaggat tcgggtaatt tcgagagagt
     8521 tgaacagtga tccgatcaat gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga
     8581 tccgctacgt ctttcatcat cattaagtca aaacgttcct gatcatctaa ttcttctgtg
     8641 atgcctcgac taaataggtt tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa
     8701 tgttcctctt gttcgtgtat cgacaaataa atttttcgat acagtggatt ttcgatggca
     8761 tgagtttctc taaaggaatg ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg
     8821 ttgataggaa gttcttcggg aagatctgct tggaaggtta tctgaatatt ccgtttggca
     8881 gctaacttct ggtaacgcgc taagataaga tttgtcacct ggtggttgca aaaagaacac
     8941 agcatttcga aattttttgc ttcttctaat atcttttcca catagttttt agcttttgtt
     9001 tggtccgtat gcattaaatc ttgcagattc tccatctctt ggacaaattc caggcgaagt
     9061 gtatttactt cttggatatg ctgtcgaagc tgctcataat gttgctccaa caaatcattt
     9121 ttgattttta agaaatctac ttgtctccga tctttgtcgg tatttaagat gtgatcgatt
     9181 agattgtagc tgataataac gattagaatg ataatatata aaatcgtaga ccaattgatc
     9241 gttgtttgat agatacctaa tgccgtttgg atgtaaatca agcaatggat aatggctacc
     9301 aagacgatcc aaggtgtaca agctacataa aaacggttat tcttatatgc ttcgccaatg
     9361 catgcaagtg aagtgactaa agtgcttata acactagcaa tattagcaat cggcattaat
     9421 tcaggaagtt ctgcaggagt aaaagttgca taaagcagaa cgccgataaa aatcaaaagt
     9481 tggaacgtac aaaaaattcc gtaccatttt ttatagtaaa acattcggct ccaatagtag
     9541 cagatgatga aagctgacgt taagataata aataaatgag ataaaaaaga atgaacgaat
     9601 ggttcaaaaa gaataccact taaaatatct tctgatacag cttcaaatcc taatgccaaa
     9661 gcaaagcaac ttaagagaat aagtgagtat tcaagacgtt tttgtttata catcaagtag
     9721 atcgtataga aaaaggtccc tatagcgata aaaacagcga ttatcatcgt caatacttgc
     9781 ggcactgatt gtgaataaat aaaactaatc aatggtccag ttgttcctag ataaactttc
     9841 ggtggcagtc cagcatagtt ctgataagga gatgagactg taagggtcaa tttttttccg
     9901 atataatttg ctggtaaatc tatcacagcc aatgagagtc cgggattgct ttcttcctga
     9961 taatctttcc gttggtataa aatcttttcg cctagatata cagtaatcca ttgatggttt
    10021 cctttgatta gtaaatttgc ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat
    10081 gtttcgtctt tatccacggt aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca
    10141 ggatcatctt ttgtatagaa aacccacgaa tcttttattt ctttcatttg attgctgtcc
    10201 gcaggagaaa aaataaaata aaagaaataa gcaattcctg ttaaaaataa caatgataat
    10261 ataatgatta tgctccactt tatacgtaaa
//
LOCUS       DAJSVD010000095        10171 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSVD010000095 DAJSVD010000000
VERSION     DAJSVD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.097"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            64..2760
                     /locus_tag="ON498_002197"
     CDS             64..2760
                     /locus_tag="ON498_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3940435.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2837..3325
                     /locus_tag="ON498_002198"
     CDS             2837..3325
                     /locus_tag="ON498_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3940436.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            3337..3831
                     /locus_tag="ON498_002199"
     CDS             3337..3831
                     /locus_tag="ON498_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940437.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3852..4661
                     /locus_tag="ON498_002200"
     CDS             3852..4661
                     /locus_tag="ON498_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940438.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4658..5479
                     /locus_tag="ON498_002201"
     CDS             4658..5479
                     /locus_tag="ON498_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3940439.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5530..7629
                     /locus_tag="ON498_002202"
     CDS             5530..7629
                     /locus_tag="ON498_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR3940440.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7626..8744
                     /locus_tag="ON498_002203"
     CDS             7626..8744
                     /locus_tag="ON498_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3940441.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8783..9661
                     /locus_tag="ON498_002204"
     CDS             8783..9661
                     /locus_tag="ON498_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940442.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9852..10004
                     /locus_tag="ON498_002205"
     CDS             9852..10004
                     /locus_tag="ON498_002205"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3940443.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         3122 a         1670 c         2277 g         3102 t
ORIGIN      
        1 agataaatta ttttgtcaga tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa
       61 aaattggaat acaaatatag aatagacata ttgcagccga tagtttatta tgcttttaga
      121 cacattaaac tggaggaaaa aatgaaaaag agcgctaaag atcgagtttt tgatgagctg
      181 atacgactaa cagggaatat gtcttctgtc actgccgaga aaatttcgaa gaatctaggt
      241 atctctcgtc agaatgccag ccattatttg acgagattag tggaagataa aaaggtcgag
      301 aaattaccgg gaaagcctgt tttgtggaag cctttagatg aatatgcggt tgtagataat
      361 acggagcaaa taaacgaagc ctttcattct gtagttggtc atgacggaag tctgagagag
      421 gtcattcaaa aatgtatagc cgctgtcaaa tatccgccta acggactcag tgtattaata
      481 aatggagcca cgggtgttgg aaaaagtttt ttagccacaa aaatttttga atacgctatc
      541 catgagcaaa ttattgaaaa agatgcacca tttgctatct taaactgtgc cgattatgcg
      601 gacaatcccg aactgctttc ggcaacactg tttggataca agaagggggc ctttacgggg
      661 gctgaaaagg ataccgatgg tttattggca acggccaaca acggctatct ttttctcgat
      721 gaggtccatc gcctatcgaa agaaaatcaa gaaaagctct ttctatttat tgatactgga
      781 aactatcgtc cagtcggtga aaatgtaaat tggcattctg ctaaggttcg ttttattttc
      841 gccacgacgg aaaaaggtga aaattattta ttagacacct ttgatcggcg tattcaaatt
      901 agtgttatgt tgccaacttt tgaagatcgc cccattcgag aacgattaga gctgattcaa
      961 ttattttttc aaaatgaagc ggatgtttta caaaaagata ttattgtttc aagagaagcc
     1021 ttgtctttaa tgttatctca tccctttagt gggaatattg gtaagctgaa gaatatcatt
     1081 aaaatcagtt gtgcggatgt gtatagtcga acacaagaag aaccattaac gattgggaaa
     1141 aatgagatct tgattcagtt aaatatgctg gaatccgaag tagaaagctt gccgattgct
     1201 agtttattga taaagaaaaa tcaaaagata aaaacggatc gaatcaaacc gtttgattat
     1261 gattcgattt tggaaaaaat tactcagctg ataaatgaag gtaatttcaa tgaaaatttt
     1321 tcttcgataa agaatgaagt acagcatttg gtgcatcaca ttgataagga ttctttttta
     1381 tcaatgagta gaaaattgat gattcagatg tttagaaagg tctgggtatc cgttttagga
     1441 aaaacgtacg gattgacgac gagtggacct gttgcagaaa ttattagcag atcctatgtc
     1501 atttccccaa gctataagac agacttaacc gggataaatc gtacattagc tgcaaatcta
     1561 cctcggacga cttacttatg taatcagttc ttggagcatt taccaccgct ctcagaaaag
     1621 gacgagaact ttctaagact gcttattgtc gtgactttat ctgattatgt agatgaatcc
     1681 atcgagctga agggcctgtt gcttgctcac ggagatagta cggctagcag cattcaagca
     1741 gtagtgaatc aattatgcgg caattatgtt tttgaagcgt tggatatgcc agtagagagt
     1801 aatgtgtcag agattgtcga atcgacaagg aatttcgtga aacggcaagt gaatacagat
     1861 agtctagtgc tgatcgtaga tatgggctct ttaaatcaaa tttacacaca gatcaagaac
     1921 gatttaaaag gggagttact tcttctgaat aatttgacga cttccattgc acttgatatt
     1981 ggcttgaaga tgacgatgaa tgcgccattt aaacagattg cggaggatgc agcagacaag
     2041 tattcaatca atgttcaata ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc
     2101 tgtatgtcgg gtttggggat ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg
     2161 gatgaatcca tcgaagtttt cacgaaggac tacagagaac tacgtgaatt aatcgaccaa
     2221 aatgatgaaa cttattttga caagacaaaa ttggtcatca cgacttcgga tcttccacgt
     2281 tccttttcaa ttcctaatat taatgtttat gacatcttag acggtgatgg agcagccaat
     2341 ttggagcata ttttgagtcc tgaaatgaag ccggcagaat ttaaccgctt gattcaggag
     2401 ctagtaaaat tcttttcgtt ggaaggaatt gcggaccgtt tgaactttct gaacccaacg
     2461 atcgtgatga gtgaagtgga aacagtcatt tctaaatatg aaaattacta tcatttcact
     2521 ttaaatggcc gtgtaaaact aaatctctat atgcatacgg cattgatgat ggaacgttta
     2581 ttcttggctc ggacagaaaa atctgaagaa ccgaattatg atttgaaacc agatgaagat
     2641 gaattttatt atgtttctag aagtattttt caatcaatgg agatgaaata taattttaaa
     2701 gtcaatgctt atgagctttc gctgctctat gaattattag gaccgtatat tcaaaaataa
     2761 agtgtctaaa aaagtgtcta atctgttttt tttaggttag acactttttt taatgtgaat
     2821 taacgtgatt aaaccaatgt ttttaaaagt tggcacagga attgcttata taaaagtgaa
     2881 cagaaagaaa ggaattaacc atatgaagaa aattctaata gcgactcacg gtcacttagc
     2941 ttcaggcttt cttagcagta ttcaattact gacggggaag gttcaagaga ttacggtgat
     3001 taatgcttat gtggatgagt gtgattttga acaggagttg aatgtatttg taaaccaggt
     3061 gaacgagcag gatcagattt ttgtttttac cgatttattt ggcggaagtg tcaatcagaa
     3121 gattaccaaa acctttttgg agaaagagat tgctgtaact attttgtctg gctttaatct
     3181 gccaattctg ctcgaaatag tattggcaac caaggaatta acaaaggagg atattcagga
     3241 actggtaaat aaatgccaac aggaattaaa gatgaccatt cttgatgagg tacatgtagt
     3301 aaatgaagag gaagattttt tttaggagga agaaaaatga ttaaagcagt gcgaattgat
     3361 gaacgattaa ttcatggaca agtagcgatg acttggacaa agagtctaaa tttaaccggg
     3421 ttagtagtag caagcgatga ggcggctacc aacgatattc aaaagatgac attgaagatg
     3481 gctgcaccat cgaatgtcaa agtcattatt aaaacagttg atgacgcgat tcatttgtta
     3541 aaagatccgc gggcgaagga catgcgcttg atggttttag tccctaatgt caaagatgcg
     3601 gtaaaagttg cgaaagcttt cccaacggaa atcgaattaa tgaacgtggg gaatgctggg
     3661 aaaatgacac cgccatcgga agataagaaa accttatcaa aggaagtgat gttaacgacg
     3721 tcagaaattc aagacttaaa agaattagtt gaactttatc cagataccgt ttttcagcct
     3781 acaccggcga tggaaaaacg ttcagctatc tctattttga gtgattttta gaaaaaggaa
     3841 ggagaaaaat tatgtatgat gcatttgtag tcgctttggc cgtttttatt ggagttgctg
     3901 gacatgaaat gttcgggatg tccatgctga gtcgtccgat tgttgttgct ccgttagcag
     3961 gagcgttgat gggggatttg cagacggggt tgattgtcgg ggcgtcacta gaagccatct
     4021 ttatgggtgt cgtcaatatc ggaatctcta gtacggcgga gccttctttg gctgctgggc
     4081 tggctacagc gttcgcaatc aaaatgggag gaaacgtgga tgcgattatt cccattgtct
     4141 ttccactagc agtattagga ttacagttaa tgaatatgat tttttctttt gtatgtggac
     4201 cgatggctgc taaatttttc aaatttgcaa aatctggtga tcaaaaagga atcatcaatc
     4261 tgcacttctt tatgtggttt gtccactatg cgctttatgc tcttattcca ttctttgccg
     4321 ttttatttgg agcagatgtt gtcaaaagcg tcttggataa tattccagag gtcatcatga
     4381 acggattaac agtcgctgga aatctgctgc cggcagttgg gatggcgatg ctgttacgga
     4441 tgctttggag taaaaatatt gctgtgtggt ttttctttgg ggccgttgtt atggcgtatc
     4501 tcgagttacc attgattgca attgccgcag ttggaacgat tattgcaatt gttatagccc
     4561 aaagagatct acaactaaaa aatatatctg ttcatgcagt tcaaagcgat gctgctgtaa
     4621 atgcaacgct tgaccaagaa gaggaggatt tctttcaatg aaattagctg atggaatttc
     4681 taaagatgag aaaaaattgg ttcggaagat gttttggcgc tcgatgacta tgtatgcttc
     4741 ggtgaatccg gtgacgatgg gcggcggtgg tttcgcctat tctatgatgc ccttcattaa
     4801 tcatttctac aaaagtgaag aagatcgagc tgcggcgctg gaacgtcata cacagtattt
     4861 ttcgacgacc attccttgcg cgtcctttgt catggggatt gcggcatcga tggagaagga
     4921 aaattctgaa aaagctggtt ttgacgtgga aagtatcaac gcgatcaaaa cgagtttgat
     4981 gggaccattg gctggaattg gcgattcatt gttctgggga gtttggcgtg tcgttaccgc
     5041 gggactagcg atcaacttag ccaatgccgg aaatattttg gcaccaatta tctttttagt
     5101 tatgttcaat atacctaatt acttgatgcg atactatggc ggcgttttag gctattcatt
     5161 aggctctaaa tatattgaaa agctgtatgc gaacgggtta attgaaattc tgacaaaagc
     5221 tgctagtatc ttgggtctga tcatggtagg tgcaatgacg atcagtatgg tcaaattcaa
     5281 taccgttttg agcttttcaa tgaagggcga agaactaatg aatgtccaag aaatcctcga
     5341 tagtattttt aaaggaattg taccgttatt ggtaacattg agctgtttca aattactaga
     5401 taaaaaagtc ggcgtcatca aaattatttt tggaatggtc gtcttgggaa ttttattgtc
     5461 attacttgga atcgcatagt aggaagctgc agctactagg ctgcagtttt tttagcaagg
     5521 agggaaaaga tgactattaa tgaagtaatt tattcaccca aacacccatt ttttaatgct
     5581 gagttaaacg acggaaatgt gactacttca aacgaataca gctactatcg gaatattcct
     5641 gatgtaaagg gattgacctt cgatgtaagt aatcaaaaga cattagcaaa ccttgatgtt
     5701 tgcgggacga ttcaaacgtt gaccttttat cgagaaaatt tactaactga ggaaaaacca
     5761 ggtgtttggg tcaataaaca gctctctcaa accaattcgt tatccttaaa ggttgaagta
     5821 aacgggattg tctaccattt gtcagaatcg gatcatcgta ttgaagtcga tgtgattcaa
     5881 gactgtttgc ctagatatat tcatcattat tcgacgttta aggtcattgt agtatccttt
     5941 gctcccatac tggctaataa gcgtttaagt atgcttgttc aacaggttta cattgtgaat
     6001 gaaacacacg aaagccttca agtttcagtg aaagatgtgc cttcgtatca agagaagtat
     6061 tcggatcagc aaaatgtgtt gatttctcaa aaaggaaata aggacacgat cgcacaacag
     6121 gaagtggcaa gcttcgccat tgcttttgtg gacccaaatg cttttgaaga agtaatgact
     6181 tttcaggaat ctgatatcga aaaatggctg aaggaaacat tgaactattt tgctcagcta
     6241 tttgggcagc tgtcaatgcc aaacgataga gttgtccatc tctatcgtcg tgcactttat
     6301 caaagctttt ctagtttcgg tatgaatcga cagggacagg tcgtcggcag caattggggc
     6361 agctatcctg ctactaatcg aatttggaat aaggatatgt attacgcatc attgccattc
     6421 accatgtttg aaccggagtt atgtcaaaaa acggttctat ggtttgatca atatggcgtt
     6481 aagtttcctg gttcaaaatt tacaggaggt atcaatcatt ctctgagtaa ttcgttatcc
     6541 agtgcgttac tggctagtct ttattttgaa catactgggg atacaaaatt ctttgagcgg
     6601 tgtccgcagg ttctactgaa tgcttcaaga attattgagg acatattagc tcaacgtcgg
     6661 tcaggggaac caatgctttt tccatctgtt tggttatcag acgcctttgc acttggaaag
     6721 tatcataccg gcagcaatct ttgtctttgg aaggcctgta ttggacttgc agaaattttt
     6781 gaagccctag aacaactgag tcttagcaag cgatacaaaa acattgcctg taaactaaag
     6841 gaatcaattt gtaaacaaat gactatcgat ggtccttttg gtgagcaatt tttggaggga
     6901 atcggtgatg aggagaaaac aacctattca gtgaagcact atcaaaaacc gattcttgag
     6961 caagggttaa tctttttaag tgatgtgatt gtggatggaa aaattaattt attgatgcat
     7021 gatggagaag aatcagacac tacgttgatg cctttttatc aatttttaga taataaagat
     7081 catttatatc agaacaccat ggccttttct gctagttctt tcaatccaac ctatagcgag
     7141 gaaatcaaag gcatcacttg gggattagaa tccggcgcca cttttcctgg ctttatcact
     7201 gttttaatga gcgagctgca caaccctcaa gctttcgtaa cccgattaga agaactaatt
     7261 tgtttggcag atttagacgg ttcgtggtgg tggtggccgt atcgcttaga ggctcaacaa
     7321 ggagaagtcg tgcgagactt tggctgcggc aaatgcggct gggcatcggg tctttttgtc
     7381 agcctaatga tcagtcaata tttcggattg aaattcaaaa aaggagtatt gcaaattgat
     7441 cctgttgata atttaacgtt ttcttggaag aatttgaagt tcggtcaagc cttcatttcg
     7501 attgaatgta cgcaatccga gttgagtatt tcaaatttgt cagaaaagcc attgaaaata
     7561 agtgatacga aaaaaattct tcacgtagaa aagggaaaaa cgattcacat tcaaaggaga
     7621 aaaagatgag tatactaaaa aatattaccc aatttaaaaa aatccagagt cgagcaatca
     7681 ctcccgaaaa tccaacaggc gagaaaggta aagctgcgat gttgccaagc gagctaggac
     7741 caagccgcaa aggtcgtggc gcaattccgc tgacttctgg tgaagaaact gtgattgcgg
     7801 atattactgg aacgggaata attcgtcata tgtggatgac gattcgtgat cgaacagaat
     7861 ttggcagttt tgttttacgt gatttaatct taagaattta ttgggatggg tcggatagcc
     7921 ctgctgttga aagtccttta ggggatttct tttgtaatgg gtttggagag cgttgcgagg
     7981 tgaactcgtt gccgatagtg gttaatccta cagggggctt taattcctat ttcgaaatgc
     8041 ctttccaaac atccgctaaa attacgatcg agaatttaca tccgaaggat atgtcctcat
     8101 ttttttatac gataaattac gctttggtgg acgaattgga agaaaatagc ctatattttc
     8161 acgcaaattg gcatcgggaa cgcattacca aattacagga ggattataag ttaattgatc
     8221 gattaaaggg tcaaggctat tacgtgggta cttaccttgc gcttacctgc ctagaacgtt
     8281 attggtgggg cgaaggcgag tttaaattct acattgatgg ggatagtgag tatccgaccg
     8341 taacctcaac aggttcagaa gattattttg gcggggcgtg ggcctttcat gagtacgatg
     8401 agttcggacg tccacatgcg aaaaactatt caacagcctt tttagggtat ccttaccaat
     8461 caaatagaga tgccacaaga gatcgttttg aaacgggaaa attgaatgcc gttcatgcct
     8521 ttggtgacga cgcaatgcca cgacatggtc tttatcgttg gcatcttcaa gaccccatct
     8581 gttttaccga agatttatcg gtcactttac aacaaatcgg caacgatgat gttcgcttat
     8641 ttgagcggca agacgatgtt gctagtgtcg cttactggta ttcaacagaa gcttcaggaa
     8701 atactaaacg tcatttcact gatcgagaac gcttgccgcg ttaatgaagt gtttttcaca
     8761 tattcgaagg gaggaaccta tgatgatata tggggcaatt gaagctggag ggactaaatt
     8821 tgtctgtgca gtcggtactg aggaattaac gattttggaa agagtcagtt ttcccacaac
     8881 aactccagtt gaaacaatga agcaggtcat tgattttttt aaacctttta aggaacaatt
     8941 ggcaggcatc ggggtcggct gctttggtcc aattgatatt caatgcaaat caacgagttt
     9001 cggtcatatc acatcaacac caaaacttgc ttggcaaaat ttcaattttg taggaacgct
     9061 aaagcaatat ttcaatattc cgattgaatg gacaacagat gtcaatgcgg cttgctatgg
     9121 tgaatatgtt gctggttctg gcaaagggtt agcaggtgtt gcatacttca ctgtgggaac
     9181 aggtatcgga ggtggtgcct tgttcaatgg gactttcgtg gaagggttca gtcatccgga
     9241 aatggggcat atgttgatca agcgacatcc aaaggacaat ttttcaggaa actgtccctt
     9301 ccaccaagat tgtttagagg ggatggcagc aggaccagcg attgaaaaac ggttgggtgt
     9361 aaaaggccag aatttattgg ctgacgattc gttttggcag attgaagcct tttaccttgc
     9421 ccaatgtgca tacaatacaa cactgatgtt ttcgccagat agaattattt ttggcggtgg
     9481 tgtaatgaaa caagaacaca tgaagaaaaa agttcaggat aaatttgttg agcttataaa
     9541 tggatatgtt gaaataccac caattgatag ctacattatt acgcccgaat taggtgataa
     9601 tgccggaata atcggcggat tagcattggc cagaaaagcg gtaagaaata agcaaccata
     9661 attaatgaat gtttcattga tactcatgtc aaaagtattt aaccgagtaa tcaaatgatc
     9721 aaatgaagtt ggataaagcg aaaatgaatt caaataaaaa ggctctaagt caaatcgtgt
     9781 tgttttcatt aaatcactga aaggaaatgc ttctgcgttt ccttttttct ttttgttgtg
     9841 cgctgtatac cttgaggata ttcactggtg gcgtacactt aaatagtaat atattgtgtt
     9901 actttatgtc tgtggtaata gttatagttg cttaccaatg tatgttcatt tttcgaaatg
     9961 ttgacttcat aatccttggc ttaatgtttg aatttttttg gtgatagatt ttttcagccg
    10021 tataaaataa atcgtaattc tattatagag agttgtgatt ataaaaagat agcaagaaaa
    10081 aaaaagaaag tttctataca ttttaaatat tttattccta tttctattgc taaaaaagta
    10141 gaaattctat atttaccttt aactctcgct t
//
LOCUS       DAJSVD010000096        10142 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSVD010000096 DAJSVD010000000
VERSION     DAJSVD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.098"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            286..555
                     /gene="rpsN"
                     /locus_tag="ON498_002206"
     CDS             286..555
                     /gene="rpsN"
                     /locus_tag="ON498_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3940444.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            570..749
                     /gene="rpmF"
                     /locus_tag="ON498_002207"
     CDS             570..749
                     /gene="rpmF"
                     /locus_tag="ON498_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3940445.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            781..1158
                     /locus_tag="ON498_002208"
     CDS             781..1158
                     /locus_tag="ON498_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940446.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1179..1328
                     /gene="rpmG"
                     /locus_tag="ON498_002209"
     CDS             1179..1328
                     /gene="rpmG"
                     /locus_tag="ON498_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3940447.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1352..2335
                     /locus_tag="ON498_002210"
     CDS             1352..2335
                     /locus_tag="ON498_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3940448.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2365..2487
                     /locus_tag="ON498_002211"
     CDS             2365..2487
                     /locus_tag="ON498_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3940449.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2505..4028
                     /locus_tag="ON498_002212"
     CDS             2505..4028
                     /locus_tag="ON498_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3940450.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4052..4291
                     /locus_tag="ON498_002213"
     CDS             4052..4291
                     /locus_tag="ON498_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940451.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4484..4945)
                     /locus_tag="ON498_002214"
     CDS             complement(4484..4945)
                     /locus_tag="ON498_002214"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3940452.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5140..5358)
                     /locus_tag="ON498_002215"
     CDS             complement(5140..5358)
                     /locus_tag="ON498_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940453.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5476..7092
                     /locus_tag="ON498_002216"
     CDS             5476..7092
                     /locus_tag="ON498_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3940454.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7333..8673)
                     /locus_tag="ON498_002217"
                     /pseudo
     CDS             complement(7333..8673)
                     /locus_tag="ON498_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8949..9104)
                     /locus_tag="ON498_002218"
     CDS             complement(8949..9104)
                     /locus_tag="ON498_002218"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3940455.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9400..9732)
                     /locus_tag="ON498_002219"
     CDS             complement(9400..9732)
                     /locus_tag="ON498_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940456.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3371 a         1609 c         2006 g         3156 t
ORIGIN      
        1 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
       61 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
      121 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
      181 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
      241 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
      301 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
      361 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
      421 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
      481 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
      541 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
      601 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
      661 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
      721 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
      781 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
      841 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
      901 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
      961 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
     1021 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
     1081 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
     1141 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
     1201 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
     1261 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
     1321 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
     1381 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
     1441 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
     1501 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
     1561 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
     1621 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
     1681 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
     1741 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
     1801 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
     1861 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
     1921 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
     1981 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
     2041 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
     2101 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
     2161 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
     2221 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
     2281 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
     2341 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
     2401 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
     2461 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
     2521 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
     2581 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
     2641 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
     2701 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
     2761 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
     2821 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
     2881 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
     2941 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
     3001 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
     3061 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
     3121 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
     3181 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
     3241 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
     3301 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
     3361 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
     3421 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
     3481 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
     3541 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
     3601 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
     3661 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
     3721 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
     3781 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
     3841 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
     3901 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
     3961 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
     4021 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
     4081 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
     4141 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
     4201 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
     4261 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
     4321 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
     4381 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
     4441 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
     4501 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
     4561 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
     4621 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
     4681 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
     4741 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
     4801 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
     4861 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
     4921 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
     4981 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
     5041 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
     5101 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
     5161 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
     5221 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
     5281 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
     5341 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
     5401 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
     5461 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
     5521 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
     5581 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
     5641 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
     5701 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
     5761 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
     5821 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
     5881 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
     5941 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
     6001 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
     6061 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
     6121 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
     6181 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
     6241 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
     6301 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
     6361 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
     6421 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
     6481 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
     6541 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
     6601 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
     6661 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
     6721 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
     6781 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
     6841 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
     6901 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
     6961 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
     7021 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
     7081 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
     7141 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
     7201 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
     7261 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
     7321 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
     7381 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
     7441 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
     7501 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
     7561 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
     7621 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
     7681 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
     7741 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
     7801 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
     7861 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
     7921 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
     7981 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
     8041 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
     8101 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
     8161 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
     8221 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
     8281 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
     8341 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
     8401 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
     8461 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
     8521 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
     8581 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
     8641 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
     8701 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
     8761 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
     8821 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
     8881 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
     8941 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
     9001 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
     9061 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
     9121 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
     9181 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
     9241 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
     9301 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
     9361 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
     9421 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
     9481 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
     9541 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
     9601 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
     9661 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
     9721 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
     9781 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
     9841 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
     9901 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
     9961 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    10021 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    10081 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    10141 cg
//
LOCUS       DAJSVD010000097        10138 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSVD010000097 DAJSVD010000000
VERSION     DAJSVD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.099"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            278..787
                     /locus_tag="ON498_002220"
     CDS             278..787
                     /locus_tag="ON498_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3940457.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ON498_002221"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ON498_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3940458.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ON498_002222"
     CDS             2322..3302
                     /locus_tag="ON498_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3940459.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ON498_002223"
     CDS             3424..4842
                     /locus_tag="ON498_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR3940460.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ON498_002224"
     CDS             4913..6382
                     /locus_tag="ON498_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3940461.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ON498_002225"
     CDS             6392..6859
                     /locus_tag="ON498_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3940462.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="ON498_002226"
     CDS             6895..7767
                     /locus_tag="ON498_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940463.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..>10138
                     /locus_tag="ON498_002227"
     CDS             7849..>10138
                     /locus_tag="ON498_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3940464.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKY"
BASE COUNT         3522 a         1464 c         2013 g         3139 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatatt
//
LOCUS       DAJSVD010000098        10012 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSVD010000098 DAJSVD010000000
VERSION     DAJSVD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.100"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            195..3422
                     /gene="fss3"
                     /locus_tag="ON498_002228"
     CDS             195..3422
                     /gene="fss3"
                     /locus_tag="ON498_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3940465.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3535..3849
                     /locus_tag="ON498_002229"
     CDS             3535..3849
                     /locus_tag="ON498_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3940466.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3862..4236
                     /locus_tag="ON498_002230"
     CDS             3862..4236
                     /locus_tag="ON498_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3940467.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4246..4581
                     /locus_tag="ON498_002231"
     CDS             4246..4581
                     /locus_tag="ON498_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR3940468.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4664..6004
                     /locus_tag="ON498_002232"
     CDS             4664..6004
                     /locus_tag="ON498_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3940469.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6082..6756
                     /locus_tag="ON498_002233"
     CDS             6082..6756
                     /locus_tag="ON498_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3940470.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6749..6913
                     /locus_tag="ON498_002234"
     CDS             6749..6913
                     /locus_tag="ON498_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940471.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6989..8131
                     /gene="dcm"
                     /locus_tag="ON498_002235"
                     /pseudo
     CDS             6989..8131
                     /gene="dcm"
                     /locus_tag="ON498_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8121..8411
                     /locus_tag="ON498_002236"
     CDS             8121..8411
                     /locus_tag="ON498_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940472.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8670..9854
                     /locus_tag="ON498_002237"
     CDS             8670..9854
                     /locus_tag="ON498_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3940473.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9851..9988
                     /locus_tag="ON498_002238"
     CDS             9851..9988
                     /locus_tag="ON498_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3940474.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3312 a         1684 c         2257 g         2759 t
ORIGIN      
        1 tttgaaatgt ttgtgttcta aaatgtgttc taatttaaag gcagtatatt tcgtatttta
       61 gattaaattt ttaggctcat tcattctcaa atccattcta ttcagtcaat aaaaatttta
      121 aaaagacttt ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga
      181 aaggagaaca tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga
      241 gtttggctgt tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa
      301 ttacccatcc acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag
      361 gaacttttag tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca
      421 acggctttaa acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca
      481 ctcctggcta tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg
      541 ttttatggaa aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct
      601 ggcaagaagt taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa
      661 atatcgcagc cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa
      721 ccttccataa tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga
      781 ataatctgaa tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc
      841 gtgtgaatgg taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc
      901 tgacgcttaa gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac
      961 agactttaat ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg
     1021 aaacggaggg ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag
     1081 gaacggtttt ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca
     1141 aagaaggcat tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa
     1201 aatcagtacc ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag
     1261 gcgaaaccat ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata
     1321 aaagcggagt tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata
     1381 cgtttgccat tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg
     1441 aaaacggtca tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt
     1501 atgtgaccga aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg
     1561 aattgaagta tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc
     1621 aagaagtcac tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac
     1681 aaggtaaggc agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag
     1741 ctgttaaatg gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg
     1801 aaacggtcac tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca
     1861 acgagtatta ttgccaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt
     1921 atcctgtatc catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg
     1981 ttacggcaaa agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag
     2041 ctgcgggcac taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag
     2101 ggaccaatga aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag
     2161 ggtttgatgg ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag
     2221 tagaagcccc agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg
     2281 aaaacaaaga agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa
     2341 aacaaccaat taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg
     2401 ttagtttgaa ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc
     2461 ttgcgacttg gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg
     2521 ataaattgag ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca
     2581 ttgatgtgga tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga
     2641 ttgcagaaac aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc
     2701 ataaattaac cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg
     2761 aaaataagga agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata
     2821 accaagccca aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc
     2881 acctagaaaa cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg
     2941 tgtcgattac tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg
     3001 ctttattacc ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag
     3061 taaatgataa agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc
     3121 cagaaggaac caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg
     3181 gaaaacacaa tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa
     3241 ccatactaag tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat
     3301 ctagtcccac agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt
     3361 tcattggttt tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact
     3421 aaggtgatga gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa
     3481 aagtagtcgc gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa
     3541 ttgaaatttg tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa
     3601 ggagaaatta taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat
     3661 ttgtattcca gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt
     3721 gaaaaggatt ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa
     3781 ggctatgaga ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg
     3841 aaagcatagg aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta
     3901 acatttggga agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat
     3961 ggaacaattg gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg
     4021 cgaatgattc aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca
     4081 gaggtagagt tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga
     4141 actaccgtta attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa
     4201 ggaaatccta cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt
     4261 gaacatattt tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc
     4321 gttaaagcat taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga
     4381 gaaaatagcg acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct
     4441 ttctttatag ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa
     4501 gaaattggtg tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg
     4561 aaacaactcc cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg
     4621 aaatcctatt gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg
     4681 catacgcgca ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct
     4741 gttcatcggt tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa
     4801 gctatcactt tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg
     4861 aagtctcgct ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg
     4921 gcgaagcttg tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga
     4981 agtgaaacaa actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga
     5041 gactatcagt tacttcccca aaatctgtta tcgaatgaaa gatggattac tgtctattcg
     5101 cgttcaaatt tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga
     5161 aagcggactg tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac
     5221 tttactgtat gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg
     5281 gactctgcga ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat
     5341 cgctggtggc acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt
     5401 gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg
     5461 tacggtgatg cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt
     5521 ctatgaacgt atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg
     5581 agagaattat gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc
     5641 ttatatggag atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat
     5701 cgtgatgttg ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc
     5761 gaaatattta ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat
     5821 gagtgaactc ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca
     5881 catcaaaggt cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc
     5941 acttgtacca aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga
     6001 ttgagtgatc ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta
     6061 ggaagaaagg ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca
     6121 aataaggata agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac
     6181 gcagttttta tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct
     6241 gagaattatg ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat
     6301 tataatggaa aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca
     6361 tttagatatg tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta
     6421 agtgctgaaa aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat
     6481 gtttatcaag accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga
     6541 cgtcatgtgc cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca
     6601 aaagtaaaga gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat
     6661 aatatttccg aagttcatta tgctacggat aatctagaat tatttttacc actagggtat
     6721 agtaggaaag atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt
     6781 aaagaaatac aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa
     6841 aacggtgatt aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt
     6901 aaataaaaaa tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct
     6961 tttttctttg caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg
     7021 tggctttcgt ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga
     7081 taaatttgct agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca
     7141 tgacatcaca agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg
     7201 cggcggattt ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac
     7261 tcgaggtact ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt
     7321 attccttgag aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat
     7381 cctccgaacg ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc
     7441 ctacattccc caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac
     7501 cgaacaagta tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt
     7561 ggtaaatatc aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt
     7621 agcgccaacc ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat
     7681 ctctgtagca ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac
     7741 tggaaccgcc cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa
     7801 tgaaacagaa tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc
     7861 cacaagtcgc cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca
     7921 acaagcgatc gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg
     7981 acttcagggc tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca
     8041 gttgtataag caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag
     8101 attaaaagag ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga
     8161 ttggaaagat tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata
     8221 ttcgcaagaa tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg
     8281 ctcgaattga aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag
     8341 aaccagatat tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta
     8401 cagatgagta acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg
     8461 aagcagaaat cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt
     8521 ctcacccccg tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa
     8581 gaatgcttgt tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa
     8641 gttaacacct tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat
     8701 taaaagaccg acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac
     8761 ttagtcggcc gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca
     8821 tgaggaaaat ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt
     8881 ttgactatgt aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc
     8941 tccatttgaa atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac
     9001 agtatgtcat gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt
     9061 tagaattaaa ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta
     9121 gctggtacga ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact
     9181 tggcaatcaa tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga
     9241 aggaagaatg tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa
     9301 aggctgatga aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg
     9361 tttatttctg tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag
     9421 accaaactga gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg
     9481 cgattcaaga tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc
     9541 gttacttgcg atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg
     9601 aacaatgggg gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa
     9661 aaccgtatac cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga
     9721 aaatggctaa ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga
     9781 atgcacgctt atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa
     9841 atttgattat atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt
     9901 tggtggcagt atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga
     9961 tcgagcaatg gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DAJSVD010000099         9945 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSVD010000099 DAJSVD010000000
VERSION     DAJSVD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.101"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..418
                     /locus_tag="ON498_002239"
     CDS             <1..418
                     /locus_tag="ON498_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940475.1"
                     /translation="WIFYLFLMGYGLKEWNWKEKVRYKLPQVFISLIVLFGLIILMTQ
                     ISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLSDKLGIKGRGNFLAVFLIVTQ
                     VICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            431..2449
                     /locus_tag="ON498_002240"
     CDS             431..2449
                     /locus_tag="ON498_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR3940476.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2509..3225)
                     /locus_tag="ON498_002241"
     CDS             complement(2509..3225)
                     /locus_tag="ON498_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3940477.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(3452..3994)
                     /locus_tag="ON498_002242"
     CDS             complement(3452..3994)
                     /locus_tag="ON498_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3940478.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3999..4226)
                     /locus_tag="ON498_002243"
     CDS             complement(3999..4226)
                     /locus_tag="ON498_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3940479.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(4305..6185)
                     /locus_tag="ON498_002244"
     CDS             complement(4305..6185)
                     /locus_tag="ON498_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3940480.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(6374..6988)
                     /locus_tag="ON498_002245"
     CDS             complement(6374..6988)
                     /locus_tag="ON498_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940481.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            7130..7690
                     /locus_tag="ON498_002246"
     CDS             7130..7690
                     /locus_tag="ON498_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3940482.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7995..8309)
                     /locus_tag="ON498_002247"
                     /pseudo
     CDS             complement(7995..8309)
                     /locus_tag="ON498_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(8563..9727)
                     /locus_tag="ON498_002248"
                     /pseudo
     CDS             complement(8563..9727)
                     /locus_tag="ON498_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3062 a         1924 c         1610 g         3349 t
ORIGIN      
        1 atggattttt tatttatttt taatgggtta tggtttgaaa gaatggaact ggaaagaaaa
       61 ggtccgctat aagcttcctc aagtatttat cagcttgatt gttttattcg gtttgattat
      121 tttgatgaca caaatttcac tcatcatcag aggaaataca gatacagcgc tgaggttttc
      181 taaaccatat tctctcttcg caatgtatta tactttctct tcgtttttat ttttagaaat
      241 gctatctgat aaattaggta taaaaggaag aggaaatttt cttgctgtat ttttgattgt
      301 cactcaagtt atatgtaatt tccctttagt ttcttatcgc gtatctacat ttatgaaacg
      361 tgaatttcca aactcaggga aggcatggct gataaatatc gggcagtttt ttatttgata
      421 tttattagca gtgtccctcc tagttatctt tttattttat atacaaaaaa tccgtttcat
      481 tcatcgtttc atcgaatact tcgcttttga ttcgattgaa caattaactc agcgttggaa
      541 aaaaagaatt cattggcttt ttgttcataa aagttatttt ttagttgcag cattttttta
      601 ctgctttacc ttcgtgcaga ttttcgtttt agaaccaaaa gaaggttgga aagaaactat
      661 ccaggttgcc ttaaaaatat ttactcagcg acaagcaccc atgatcctga caatcatcat
      721 tattatggga tttttcttct tactattgtt gatttccaat cgtttttggt acgccttgac
      781 tgctaccttg atcattaact tactgctcac tatttcgaca gtaatcaaaa tggaaatgcg
      841 ggaagagcct gtctttcctt cagatttaaa aatgctgacc ggattgtcag aactattatc
      901 gatggttagc ccagtcttat taatcgtggg cggattgatc cttctcctat tattgattac
      961 tagtattatt gtacaacgta gactacaaaa acagtattcc ttgaagattc attggaaacg
     1021 tcgatttatc agtatcgctg tattattagg atgtttttcg ggtgtctttt tcattaatca
     1081 caaaaattcg ccttctttct tgctgtttaa tttatttaaa gtaaacaaga ctttctttaa
     1141 ccagcaaggc gccgtaaaag ataacggacc agtcattcaa ttcctaaaca atatcgacat
     1201 taaaatcatg gaagaacctg ctggctattc gaaagaaaaa attaatggaa taatgaaaaa
     1261 gtacaacaca aaggcagatg aaatcaacca aacaagaagt gactggttaa acaatcagac
     1321 gatcgttctc aatttaagtg aaagcttcag tgatcctgca cgtgtcccta atctgacagt
     1381 acctacagac cctattcctt acatcaaaag cattataaca gaaggaacag gaggattgat
     1441 gttatccgtc ggttatggtg gcggaacagc taacatggaa tgggaaggac tgacaggctt
     1501 gagtatcagt aacctgtctg cttcgttagt tacaccttat acccagctag tggagcgtca
     1561 gaaaataagt ccaaatatta ccgatctatt cgatgaaaaa atagccatcc atccttttac
     1621 agctgctctt tatcgcagaa aacaagtgtt tgataaattt ggttttgaca ggttctatta
     1681 tttagacagt ccggataaat taacttatac ggataaaata gaacaaagcc cacgcatatc
     1741 ggatgattca gcatacaaag aaaccttgaa agctttacaa agcaatacgg aaacgtcaca
     1801 attcatccaa ttatccacta tgcaaaacca tatgccttat gatgattact atagtgaatt
     1861 gaactacact gctgaaggaa ctgctgtcat tcctaataga agaaaagaat tacaaactta
     1921 tatgcaaggc cttcactata ccgatacagc agtaaaaaaa ttcatccaag aaatcgacaa
     1981 aatcgataaa ccgattacct ttgtttttta tggtgaccat ttacccgcaa tctatagtgg
     2041 gaatgattcg aaaaaatatg gattagaaca gcaccagaca gattatttca tttataacaa
     2101 caagtatagt cgtgaacaag cagctacgtt aaataaagaa gttgttgctc ctaataactt
     2161 tggtgctatg gctcttgagc atgcgaatat caaagtaact ccttactatg cgttgttgac
     2221 agaagtatct aatcatctac ctgcattaac aattgatcct acagaaagct tgtctaatcg
     2281 ttttaatgga aaacaggtct ttgttacaga ggatagtcaa attatccaag aaaaaaattt
     2341 aactgagaaa caaaaagaaa ttctagaaga ttatcgtctg atacaatatg atcttgttgc
     2401 aggtaaccag tacagtgcaa catgggcgac acaaaaaata aaagactaac aaataaactg
     2461 agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt aatttgcttg
     2521 tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct cgttgaagta
     2581 aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg gctgattttg
     2641 ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg catcggaata
     2701 gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt ctgcagaaac
     2761 aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag ggactttaag
     2821 accatataag tccacactca tttgattcgt ataacttttg tattgttgtc ccattgtttg
     2881 gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc acagtgtttg
     2941 ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag aacttgaagt
     3001 agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg tatctgctgt
     3061 ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag ttgcaactgt
     3121 tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg tacaaccagc
     3181 taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa aaactccttt
     3241 cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg atttttcgta
     3301 ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga tgttgtctgt
     3361 aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt tacctcacgc
     3421 tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc tcatccaaat
     3481 cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct ttattgaaac
     3541 ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca gctaatttga
     3601 ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt tccaacattg
     3661 ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa cgcatgactt
     3721 cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag attttattga
     3781 accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct tgcgaagatg
     3841 tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta aaatttgcta
     3901 atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct gcttgttttg
     3961 tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt tacttttgct
     4021 gattggactt cataacctaa tttttccaca actgcagcta attgttcagg gtggacttca
     4081 tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt attgcttacc
     4141 ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca agctagtgta
     4201 tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt gatagtctta
     4261 ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga tttttcttct
     4321 ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat caagatacta
     4381 gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa gccgttgagt
     4441 aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa aacagttttc
     4501 ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa gacaacgtca
     4561 ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc agccgcagcc
     4621 aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc ttgttcttgg
     4681 tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac ttcatcaatg
     4741 cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag catcactaac
     4801 tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg tcgaatcaca
     4861 tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat gaccgagctg
     4921 cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga aagtttgtga
     4981 gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc agctgtgatt
     5041 ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc aatgtctttc
     5101 tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt ttctaatcga
     5161 gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt ggatacagca
     5221 gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat tttggaaaaa
     5281 gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt accgattcct
     5341 gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag aaccaaaaca
     5401 gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat aagtaaaacg
     5461 gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga tttggtatct
     5521 tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc gaccttagtt
     5581 gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc atctccactg
     5641 attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata ccctttcccg
     5701 tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac gatatcgcct
     5761 tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac aacactagca
     5821 gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt caatgttttt
     5881 tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc cttgtattct
     5941 ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga gataactttg
     6001 actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat agaagctatg
     6061 gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa accaaaaata
     6121 atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca cttttgtaat
     6181 ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat acttatccat
     6241 cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt ggtagaatac
     6301 actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac tattgtcgtt
     6361 tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga tctatttctt
     6421 tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca cgtcgttcat
     6481 agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct ttctctcttt
     6541 tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca ggatttgcag
     6601 ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca agcatttgtt
     6661 catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt aaaaaatcga
     6721 atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct ccttttatct
     6781 ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt tgagttgctc
     6841 gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt tcattttcca
     6901 tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg taatctttct
     6961 tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa tgattgtgtt
     7021 atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct ataagtttgt
     7081 cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa tgaaaaagac
     7141 agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca cattattagt
     7201 aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag atgtacatcg
     7261 ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat tagaaagcaa
     7321 cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct atgaggaact
     7381 atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct tatttatcct
     7441 caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag aaaactatct
     7501 gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga gattctttac
     7561 tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa gcaattacaa
     7621 gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca tagagaaaat
     7681 catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct tctcgtataa
     7741 caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa atttctataa
     7801 ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt gatatatctt
     7861 gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa tttattacaa
     7921 cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca tccagtttat
     7981 tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc atctaacatt
     8041 ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc tggatgtttg
     8101 gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg aattggatct
     8161 tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac aaaccactca
     8221 ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg tttaatgaca
     8281 aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat tcaaagataa
     8341 ttttctcaat aaagattttt gattccagta agattccttt actcctttta caacatagcg
     8401 ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag cccaattctc
     8461 tataaagaag attaagcttc attattataa tataaagttt aaatttggtt tattacttac
     8521 tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca ttatttttat
     8581 atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac ataaactaat
     8641 taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga ttgtttctcc
     8701 ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt ccatttactt
     8761 ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct ttagattcat
     8821 gtactgtata gactattttc gaatccatca cttgataagg catagtatgc cattccttgc
     8881 caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc gtgggtgcat
     8941 agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct tgattcatta
     9001 ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt gcagcagata
     9061 cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc ttagctgatg
     9121 tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga aagcctaaga
     9181 ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac catggaaaaa
     9241 atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt tctttgcgat
     9301 taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg attcctaccg
     9361 taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca tttaatgctt
     9421 ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt tgtcggaaca
     9481 tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt gacaactcca
     9541 caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg cctaagacta
     9601 gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa atgagtaagc
     9661 tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt gcgtaagcat
     9721 aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca gaacgatgat
     9781 aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata aaagccgcat
     9841 cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta caaacattca
     9901 atagccaaac atataagata taactaacga ttaaattatt actta
//
LOCUS       DAJSVD010000100         9797 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSVD010000100 DAJSVD010000000
VERSION     DAJSVD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.102"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..106
                     /locus_tag="ON498_002249"
     CDS             <1..106
                     /locus_tag="ON498_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3940483.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            583..783
                     /locus_tag="ON498_002250"
     CDS             583..783
                     /locus_tag="ON498_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940484.1"
                     /translation="MEEEKIFEKRWQLASSEQRARYNNLITSYPTVDWTYKEKKYLLW
                     LCQLDIDTFETFEVILDKIKQN"
     gene            complement(1495..2100)
                     /locus_tag="ON498_002251"
     CDS             complement(1495..2100)
                     /locus_tag="ON498_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3940485.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2282..2617
                     /locus_tag="ON498_002252"
                     /pseudo
     CDS             2282..2617
                     /locus_tag="ON498_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2782..3009
                     /locus_tag="ON498_002253"
                     /pseudo
     CDS             2782..3009
                     /locus_tag="ON498_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3135..4106
                     /locus_tag="ON498_002254"
     CDS             3135..4106
                     /locus_tag="ON498_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3940486.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4246..5559
                     /locus_tag="ON498_002255"
     CDS             4246..5559
                     /locus_tag="ON498_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3940487.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5602..6468
                     /locus_tag="ON498_002256"
     CDS             5602..6468
                     /locus_tag="ON498_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3940488.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6465..7313
                     /locus_tag="ON498_002257"
     CDS             6465..7313
                     /locus_tag="ON498_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3940489.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7327..8799
                     /locus_tag="ON498_002258"
     CDS             7327..8799
                     /locus_tag="ON498_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3940490.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8816..9688
                     /locus_tag="ON498_002259"
     CDS             8816..9688
                     /locus_tag="ON498_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940491.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3077 a         1677 c         2038 g         3005 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagacattt
      121 aaaaagaaaa gtgacagata ggaataaaaa cttattaaat gtagttatga tctgttatat
      181 ttcatttcta atactaggtc ttccacaagc agtgtttcct gaaatgtttt ctggagactg
      241 gggtattgtc agctattcta tattaacatt gattgctatg gttttaggta cttcatttta
      301 taatgctttt gtaagtaagc atgttgagga atatgaagta ttaatgtact taaaagagaa
      361 aaaaacaagt aattaatatg agaacaccac gcatagtttt tattttgaga tattctagaa
      421 tgaaacacat taagaacgaa agcaaagcaa aagacgccag aacttctact accttctaag
      481 ttttttcata tttgtttgtt atgctataca tagatctgca taattagact tgcgctgccc
      541 gttattgaag tttcaggcag cgctcttttt gggggatcga taatggaaga agaaaaaata
      601 tttgagaaac gctggcagct agcatcaagt gaacaaagag caagatacaa taatttgatc
      661 acctcctacc ccactgttga ctggacatat aaggaaaaga aatacttact ttggctgtgc
      721 cagttagata tagatacctt tgaaacattt gaagtgatcc tagataaaat taaacagaat
      781 tgatcagaaa caagcgaagc cttctctcgg ttcgcttgtt ttgctattta ttagttggct
      841 cagggcaact ctaagctcgt ttgaaagaaa accgatggat aatccccttg tttgtaccac
      901 tttagaatac atggatgtac gtattgccat tgactcctat tagttactga aaaaaaattt
      961 aaacaagttg agtattgcag ataaatagct ccagtgaggt gaataactgc tccactcaag
     1021 gcaatagata gctccatcca gggcaacaaa ctgctccact ttcatattta atagatgaat
     1081 aaccttctgt ataatagaaa ggcatacctt tcggtatgac aacttataca gaatgttatt
     1141 ttattggttc actatcgaaa aattttggag ctttattttt ccggtatttc tcaacgaacg
     1201 attagtttaa ctgtgggaag ctccagacat acgattcgag aagttacttc tcgagcagac
     1261 aggaaggggg tgactgaact aaccgctgat atgagcgata cttggctgta ggaatatctt
     1321 tttccggaaa aacaaacaac agcacgaggt taccttcctg tagactggga atatgttcat
     1381 aaagaactaa tgaaaaagaa tatgacattg aaactccttc atagagaata tggggtacat
     1441 ggaacataga aacgaaaatg cacggtagaa aagcgaatga atatagtata aattctacaa
     1501 atcattcgct ttctttattt tatatatggt tggtcgagag agaccatttt ctttggcaat
     1561 ttctgcaact ggaactcctt gatttaacat gtccaccaca gttttaaaga ctaatcgttt
     1621 ttgcggatct gttgagttct cagaatattc aattggtcgc cccttatatc gaccagcttt
     1681 tttggccaat aatactcctt gccgctgacg ttcacggatt ttctcacgtt cgttttgtgc
     1741 aatataactt agcaatgata aaaacatatc gaacatagca gtatctagga gttcatttcc
     1801 agtattaaaa tctaaaaact gtgcatctag tatttttaat gctacttttt tctgtttcat
     1861 aaaggtaact gtatttttta tgtcttcata atctctgcct aggcgatcca gtgatgcaac
     1921 aatgacacaa tcaccttctc ggatataatt tagtaatttt tgaaactcag gtcgctggat
     1981 atctttacca ctggcttttt cttggaataa tttatcaatg ttaaggtctt ccatcattgc
     2041 caattgtcga tctaaatttt gatcttgtga agacactcta atatatccaa tttttgccat
     2101 aaaaactcct tttaaaaaat gatataattt acatgtaaaa tttacttgta gttttactgt
     2161 tatcatagca tttttaaaaa taaattttaa gtctacttat aatttacttt ggaggttttg
     2221 aatgaatgaa attaagcgca ataaaaattc ttttggtgtt tatttaggag agcctagctc
     2281 ggaacagtta aataaaggtg aagctcgtca tgctctatgt cgaaatatat tttatggtag
     2341 aaaaggcaaa ctttatcaaa cttattacga cggtatggag gaacagctca acgcattagg
     2401 attagtaact aatgctatca tatattggaa ttcactatat ttagaaaaag tctttgaaca
     2461 aattaaaacg gaagggttcg actgttccga agaagtgtta cgcaagcaat ttccattaat
     2521 tagtgatcac attaattttg ttggcaaata tagtttccgc tatgaaaaag aattatcgaa
     2581 tggaatacta cgtccgttaa atatagagaa ttattgagaa tgttcgtctt ttggttccta
     2641 ccgtgcattt tcgcacggat gttccagata ctatttttga atgtataaaa ttgaaaacac
     2701 aagaatacta ataaatcaac gattcttgtg tttttaattt ttcactaccg tgcattttcg
     2761 tttctatgtt ccatgtaccc caatcttgaa aaattaatta aagacttaga taaagccatt
     2821 gacgacttag tcgtggtgct accggagtat caatgtttaa ccagtatccc aggtattggt
     2881 cctgtttagg ctgctggaat actcgctgaa attggtccaa ttgaacgttt ttctgatgaa
     2941 ataaaattga caaagtatgc cggcctatat tggcgacaga gtcaatccgg taaatctgaa
     3001 tacgaaaatt tttttacgaa aagcttcacg tctaatatct attttaaaat aaaaatattc
     3061 ctgctaattt ggaatcgttt ccttcttttg gtataataat acctgaaatt cttttgagag
     3121 gagttgttcg tgaagtgaaa actactatga aggatgttgc tgcgttagca ggagttggtg
     3181 tgggtacggt atctagagta ataaatggag taaaggtgaa agaggttacc aaagaaaaag
     3241 taatggctgc aattagtgag cttaattatg agcctgatga atatgccaga ggtatgaaaa
     3301 cgaatagtag caatacaata gctcttattt tacctacgat ttggcatccc ttcttttccg
     3361 agtttgccta ctatgtagag gaagctctca gccaaaaaaa ttataagctt tttttgtgta
     3421 atgcgaacgg cagtgcggaa aaagaaaaag aatatatcca aatggtcaag cagagtaaag
     3481 tagacggaat tataggtatt acctattcag acattgataa atatgtatca tcaaatttac
     3541 catttgtcag tatagaccgc catttttcgg aacaggtttc ttatgtaacg gcagataatt
     3601 atttaggtgg gcaaattgcc gctcaagaac tacttactcg taactgtaaa aacttgtgct
     3661 atgttggggg tgtttcacct taccctaacg aaacaaacaa tcgtaaagct ggtttttaca
     3721 atatttgtga ggaaaataaa atagctgtga aggtattgga tatgcctgaa ccaattagca
     3781 atttagagga acaattgctc gctttttttt cggataatcc tgaaattgat agcattttta
     3841 ccatcaatga tttcatggct ttggatgtga taaaagccca aaaaaaacta gggaaggaag
     3901 cacctaagga ttttcaaatt attggttttg atggagtcaa gctatcgttg caaaaagact
     3961 atttactatc aacgattgta cagccgatag aggaaatggc caaggtttca gtagaaacac
     4021 tcatggcgat gattttaaat gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc
     4081 acgctggaga gacgactaaa gagtaaaaaa ccggtttata acgatttttt tgagataggt
     4141 tgacagaaag tgatttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa
     4201 ttgaaaaata aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaagagaatg
     4261 ttagcaattg cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag
     4321 gagaaaagtt caggtagcag tgatccagga gaaattaaga tttgggttca gttttctgat
     4381 gaaacgcctg aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag
     4441 ggtgaatata aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag
     4501 gataaagtaa atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga
     4561 ccgaatactg cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa
     4621 gatgcggaca tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa
     4681 atgtatgcgt ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgctgttc
     4741 aaagatgctg gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg
     4801 gaatttcaag caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg
     4861 caaatggcgt cgggagacga aatgctgacc tacgcatata caccatttat atggtcgaat
     4921 ggcggcgatg tcgttaatgc tgatggaacc aaagccgaag actattttaa cggtaaaaaa
     4981 tccgttgaag cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt
     5041 cctattgaaa aaggatttga aactggcaaa tatgccatga tgctgagcgg ttcttggaca
     5101 atggcagatt tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt
     5161 tcagatgcaa ctggggagtt agtttcacca accggaagct ggcagttagc aatgactgag
     5221 aaaacagaga aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct
     5281 agtcgtatta tgagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta
     5341 attaaagatg aagtgtcgga aggaatgcag gtattaatta agcaaaacca actttctgga
     5401 catgcccgcc cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt
     5461 caagatatta gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct
     5521 gctgaaatgc aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat
     5581 caagtgttaa aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca
     5641 gctttgctgc tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc
     5701 tttactgatt attatttgtt aacgccagat gctagacaat ttatcggctt agaaaatttt
     5761 aagcgactgt tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt
     5821 gtttggatta ttccggtcca aataggggca gctttaggaa tggcacttat tatcaacaaa
     5881 aatcgtaaag gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta
     5941 gtcgttattt cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg
     6001 ctatttgcaa agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg
     6061 tatacgcttg ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta
     6121 gggggaatgc aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct
     6181 aagtttcagc aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt
     6241 ttacttacca cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa
     6301 ggtggaccaa tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca
     6361 gaccgtatgg taggctactc cagctccatt gcgttaatct ttaccactat gattggttta
     6421 attacaattg ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg
     6481 gaccgctaat gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc
     6541 cgatgctgtg gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag
     6601 cttccttcaa agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag
     6661 aggttatttc ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa
     6721 cctttgcctg tgggtcaatc attgtaaact ctttggcggg ttttgccttt gcgaaggtta
     6781 atttttcagg caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg
     6841 agacggtttt aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct
     6901 tggcagtgat tttaccagcg atggccagcg cgtttaatat ctatctgttt agaaatttct
     6961 ttatcgcgat tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta
     7021 aaattttctt tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct
     7081 tatcctttat ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca
     7141 gcaaattttc aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca
     7201 atcaagtgat ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg
     7261 cacaaaaata tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa
     7321 tagtgaatgt tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca
     7381 gtcaatcaag aataccggaa taaatttcat ttgatggctc cgattggctg gatgaatgat
     7441 ccaaatggtt ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac
     7501 gacagcatct ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg
     7561 gagcatttgc cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc
     7621 ggcagtgcca tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg
     7681 gaagggcaag ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa
     7741 aaatatgccg gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa
     7801 gatttccgcg atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact
     7861 aaaactgcag atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg
     7921 gagttttatt ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca
     7981 gacttgtttg aactagatgg caaagaagtc ttgattcttt ctcctattca aatgactcca
     8041 caaggcaatg cttaccaaaa tacgaactca actgttgctt tcatcggtac tgtcgattgg
     8101 caaacaggca aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat
     8161 gcaccccaga cctgtgaaaa cgaagcaggc caaagaataa tgattgcttg gatgcaaatg
     8221 tggcagcgta aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg
     8281 cctagagagc tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat
     8341 caagtaattg atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgcttctta
     8401 ctgaaggact tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca
     8461 acgaaactag aacttcacta tgcgaaggga cccgaccata cattactgct taactatgat
     8521 gttgaacggg agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct
     8581 gaaaaggaag tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc
     8641 atcgaaattt tccgtgatac ttcagctatt gagctttttc taaatgatcg tctgactatg
     8701 agtacaactt tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa
     8761 gtaacctttt cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat
     8821 ttatggttca atcgaagcag gcggcacaaa atttgtgtgt gccattagcg atgaagaaat
     8881 gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa tacctttagt
     8941 cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg
     9001 gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaagt
     9061 ggcatggcaa aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc
     9121 ttggacaaca gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg
     9181 attgagcagt gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga
     9241 agggcgcttt atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca
     9301 tccaaaagat gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc
     9361 cgctggaccc gccatcgaaa aacgcttagg gaaaaaagga caagaggttg cagaagatga
     9421 tccttattgg gaaatcgaag cctcctatat tgctcaatgt gcctacaata caaccttgat
     9481 gttttctcca gatgtgatca tttttggcgg tggtgtgatg aaacaacgcc atatgctgca
     9541 aaacgtccac gacgcatttg caaaattggt caatgggtat gtcgaaacac ctgagttaag
     9601 caaatatatc gtgaccccag cattagaaga caacgctgga acgctcggtt gtttggcatt
     9661 ggctcgagaa gcggtcttac attcataaac gaaaaaaggc tcttcgacat ttagtgttgg
     9721 tagagaaaga tacaactgaa aatttagtca taaccagtta ggcagagtgc ttaactggtt
     9781 tttgttttac ttggaag
//
LOCUS       DAJSVD010000101         9550 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSVD010000101 DAJSVD010000000
VERSION     DAJSVD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.103"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(58..636)
                     /locus_tag="ON498_002260"
     CDS             complement(58..636)
                     /locus_tag="ON498_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3940492.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(713..1243)
                     /locus_tag="ON498_002261"
     CDS             complement(713..1243)
                     /locus_tag="ON498_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR3940493.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1247..2107)
                     /gene="rlmB"
                     /locus_tag="ON498_002262"
     CDS             complement(1247..2107)
                     /gene="rlmB"
                     /locus_tag="ON498_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3940494.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2100..2495)
                     /locus_tag="ON498_002263"
     CDS             complement(2100..2495)
                     /locus_tag="ON498_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR3940495.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2492..3904)
                     /locus_tag="ON498_002264"
     CDS             complement(2492..3904)
                     /locus_tag="ON498_002264"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3940496.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3901..4437)
                     /gene="cysE"
                     /locus_tag="ON498_002265"
     CDS             complement(3901..4437)
                     /gene="cysE"
                     /locus_tag="ON498_002265"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3940497.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4523..4762)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4759..6219)
                     /locus_tag="ON498_002266"
     CDS             complement(4759..6219)
                     /locus_tag="ON498_002266"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3940498.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6389..7546)
                     /locus_tag="ON498_002267"
     CDS             complement(6389..7546)
                     /locus_tag="ON498_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR3940499.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVSSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7580..8950)
                     /gene="radA"
                     /locus_tag="ON498_002268"
     CDS             complement(7580..8950)
                     /gene="radA"
                     /locus_tag="ON498_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3940500.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9013..9540)
                     /locus_tag="ON498_002269"
     CDS             complement(9013..9540)
                     /locus_tag="ON498_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3940501.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2414 a         2144 c         1733 g         3259 t
ORIGIN      
        1 taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta
       61 ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct
      121 cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg
      181 catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat
      241 tgcttctttt aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa
      301 aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg
      361 acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt
      421 ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa
      481 agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt
      541 tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc
      601 accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt
      661 ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat
      721 tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg
      781 gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt
      841 ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa
      901 agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat
      961 tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa
     1021 caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga
     1081 agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag
     1141 cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac
     1201 caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac
     1261 gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga
     1321 cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca
     1381 ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc catttttgga
     1441 agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca
     1501 cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag
     1561 tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga
     1621 cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat
     1681 ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa
     1741 gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc
     1801 tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga
     1861 tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc
     1921 ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta
     1981 atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat
     2041 atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt
     2101 tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg
     2161 gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac
     2221 gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa
     2281 taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct
     2341 tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct
     2401 ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc
     2461 aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt
     2521 gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc
     2581 ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt
     2641 catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata
     2701 aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca
     2761 gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca
     2821 tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg
     2881 ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc
     2941 gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt
     3001 attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat
     3061 gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta
     3121 catagccatt gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg
     3181 cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg
     3241 tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt
     3301 tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat
     3361 ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca
     3421 gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat
     3481 acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta
     3541 aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg
     3601 ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac
     3661 ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat
     3721 cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac
     3781 gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac ataccgacat
     3841 gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg tagattttta
     3901 tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac
     3961 cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt
     4021 ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt
     4081 aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa
     4141 taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc
     4201 gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg
     4261 ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga
     4321 gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga
     4381 acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac
     4441 tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc
     4501 gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat
     4561 aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct
     4621 ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc
     4681 aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact
     4741 tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg
     4801 accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac
     4861 acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat
     4921 ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc
     4981 gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac
     5041 aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg
     5101 aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc
     5161 tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc
     5221 cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg
     5281 tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc
     5341 gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag
     5401 atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag
     5461 ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca
     5521 gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg
     5581 taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg
     5641 cataccatcg cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat
     5701 agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac
     5761 aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca
     5821 tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa
     5881 ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag
     5941 cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc
     6001 aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc
     6061 atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt
     6121 gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc
     6181 agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac
     6241 tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta
     6301 aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat
     6361 ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct
     6421 cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag
     6481 cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc
     6541 catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg
     6601 tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg
     6661 catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag actttattca
     6721 agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc
     6781 tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt
     6841 ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat
     6901 cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc
     6961 cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat
     7021 ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa
     7081 cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt
     7141 cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa
     7201 tcggcaatac actactgacg aatggaattt ccatattgta aagaggggtc gagatcacaa
     7261 cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga
     7321 cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca
     7381 aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc
     7441 atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc
     7501 ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt
     7561 ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta
     7621 ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt
     7681 atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac
     7741 gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt
     7801 tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta
     7861 attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc
     7921 ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc
     7981 gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac
     8041 gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta
     8101 agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc
     8161 caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat
     8221 gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg
     8281 cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag
     8341 cggttttgag tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg
     8401 tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat
     8461 gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt
     8521 tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata
     8581 catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg
     8641 atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc
     8701 ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga
     8761 cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt
     8821 ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc
     8881 gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt
     8941 tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt
     9001 atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg
     9061 atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt
     9121 gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc
     9181 ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg
     9241 cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc
     9301 tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt
     9361 tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg
     9421 aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg
     9481 cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac
     9541 cggttttcct
//
LOCUS       DAJSVD010000102         9500 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSVD010000102 DAJSVD010000000
VERSION     DAJSVD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.104"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            81..749
                     /locus_tag="ON498_002270"
     CDS             81..749
                     /locus_tag="ON498_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3940502.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            752..1795
                     /locus_tag="ON498_002271"
     CDS             752..1795
                     /locus_tag="ON498_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3940503.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(2032..2913)
                     /locus_tag="ON498_002272"
     CDS             complement(2032..2913)
                     /locus_tag="ON498_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3940504.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            3072..3800
                     /locus_tag="ON498_002273"
     CDS             3072..3800
                     /locus_tag="ON498_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3940505.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            3956..4273
                     /locus_tag="ON498_002274"
     CDS             3956..4273
                     /locus_tag="ON498_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3940506.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            4266..4874
                     /locus_tag="ON498_002275"
     CDS             4266..4874
                     /locus_tag="ON498_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR3940507.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            4929..5720
                     /locus_tag="ON498_002276"
     CDS             4929..5720
                     /locus_tag="ON498_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR3940508.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            5858..6346
                     /locus_tag="ON498_002277"
     CDS             5858..6346
                     /locus_tag="ON498_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3940509.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            6397..7269
                     /locus_tag="ON498_002278"
     CDS             6397..7269
                     /locus_tag="ON498_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3940510.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            7445..7807
                     /locus_tag="ON498_002279"
     CDS             7445..7807
                     /locus_tag="ON498_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3940511.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            7816..8568
                     /locus_tag="ON498_002280"
     CDS             7816..8568
                     /locus_tag="ON498_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940512.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            8582..>9500
                     /locus_tag="ON498_002281"
     CDS             8582..>9500
                     /locus_tag="ON498_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HCR3940513.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFK"
BASE COUNT         3082 a         1535 c         2015 g         2868 t
ORIGIN      
        1 gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct agaaattttt
       61 caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat ccgcaattac
      121 agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc gcattgaatg
      181 gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt acagatatca
      241 tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc agatttgaca
      301 cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc gggttcaaat
      361 taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg
      421 aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc ggacaaacaa
      481 aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt ctgcctcaaa
      541 aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc actcgccaac
      601 agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg atcgatgtac
      661 atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc atcacagtac
      721 ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt cacaattatg
      781 gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt gttttctcgg
      841 ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg gaaaaccaac
      901 gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag gaatatcgat
      961 ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata tgagtctggt
     1021 agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag aagtacagca
     1081 actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga cactgaggaa
     1141 tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa tccaaggata
     1201 tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat ttttatatcg
     1261 gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa aattgaacaa
     1321 actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg aacaaatcag
     1381 agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat tggatatcga
     1441 acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt ggctgaatat
     1501 catggataac gccataaagt acaatcaaat caatggacag attcatatca aactatttga
     1561 gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga atgaagagac
     1621 cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa tttctgggaa
     1681 tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac gtattgatta
     1741 tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac aataggaaaa
     1801 agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc gtttttttgt
     1861 gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc ttattgaact
     1921 acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga gagcgaaagt
     1981 catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg cttagttttc
     2041 ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac cgactttacg
     2101 agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag tattttcttt
     2161 taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat ttgcaatcat
     2221 attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa atccagcttc
     2281 gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt
     2341 ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat gatttttagg
     2401 tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc
     2461 ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa
     2521 ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt tcaaaaactc
     2581 ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga tatttaatga
     2641 aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt attgttctac
     2701 gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga tttttctatg
     2761 ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct gtttagaaat
     2821 atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg tttcggctaa
     2881 atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta ttccaataaa
     2941 gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac aatctttctt
     3001 gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta gaaaaagaga
     3061 ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg tttggcggac
     3121 taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag ctgacgatgg
     3181 tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg aaaaatatgc
     3241 cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt catttaggta
     3301 atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc ttccctagtg
     3361 aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc attgttctat
     3421 tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca ggagacagta
     3481 cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt gcgtgtaatt
     3541 tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat attttgttct
     3601 tattggcaaa atttatttat ccattgacta caccagacat gatcttagat tttaaagctt
     3661 gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa atgttcccag
     3721 tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg tggacaaatt
     3781 ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg actaaaaagg
     3841 atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac tatattatat
     3901 gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg acgtaatgca
     3961 catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa gcaaggaaga
     4021 tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca gtgaatctac
     4081 actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct atgatgaacc
     4141 ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc gtcatttaga
     4201 agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat tggaggagag
     4261 cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt cctgaagacg
     4321 atcgttttga tttgatccag tattatgagg aatatttttt agattctgga aaaacattag
     4381 atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt
     4441 acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa agacaaattc
     4501 gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt ccactagcac
     4561 tggggtttat attggttatt gggggactat tggttgcttt actagcgctc atttttagta
     4621 tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc tcaggaatca
     4681 gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt ctgttcgcca
     4741 caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg cttttttatg
     4801 tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga gatctgcatt
     4861 cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg attaccggta
     4921 ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta ggcgctcaaa
     4981 agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa
     5041 tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg gtcatacgcg
     5101 gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa ataaaggaga
     5161 aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc attagtttag
     5221 aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta tctgaattga
     5281 caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa aaagtgaata
     5341 ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca ataactgcga
     5401 aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg atcgtcacag
     5461 gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga aaagtcatcc
     5521 taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact atccaaacgt
     5581 ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg ttagaacaag
     5641 gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg acggatcaag
     5701 aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa aacagcaaag
     5761 atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt aaaataagaa
     5821 agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag aaggtcaaag
     5881 agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac taagccaaac
     5941 cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg
     6001 ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga agaaagagct
     6061 ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg ggaagaagga
     6121 acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga tggatctaga
     6181 ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa aattggatta
     6241 tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg aagacaaaga
     6301 gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa gtaacattag
     6361 atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag agcattttat
     6421 atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg attgtttttc
     6481 ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat ttttctttgg
     6541 ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca agcaaaagta
     6601 attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt ccttacgata
     6661 cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag ttctgtttta
     6721 aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt ctttaaagtc
     6781 gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt agaaatccaa
     6841 agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt caatgagaat
     6901 gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa agagctgcaa
     6961 gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca tttagcctat
     7021 gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat tttgtctgct
     7081 cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga gcaaatcgaa
     7141 gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa taatttactt
     7201 gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga catgaaagac
     7261 cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat tattgtcttg
     7321 aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat gatagttgat
     7381 tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga gagaagggga
     7441 aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac ttgcaggggt
     7501 gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa ttacgacaga
     7561 tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact atagatatat
     7621 cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg gacaattgac
     7681 tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata aaggtgtcac
     7741 ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa aactaattaa
     7801 agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa agtatacgga
     7861 aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt agaaaaaggc
     7921 gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt gaatatttta
     7981 tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga tgtgaccaca
     8041 ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat tttccaagat
     8101 ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct ttctttacaa
     8161 ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc
     8221 tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca acgggtcgcc
     8281 gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc tacaggtgcg
     8341 ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa tacaaaagat
     8401 catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg ccaacgtatt
     8461 ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca aagccgtgat
     8521 tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg ggagattgac
     8581 tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact acttggtgtt
     8641 attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa tggcaagcaa
     8701 cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt tccgattcgg
     8761 ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt cctttttgat
     8821 gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag gcagaaaaat
     8881 cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt tagtgggttc
     8941 agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc agttagatct
     9001 caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg ttttgttttt
     9061 tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca aaaaaccgat
     9121 tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata aattcttgtt
     9181 tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata tgatgaaaca
     9241 cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg tattaggaac
     9301 ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga aatcagaaca
     9361 gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc gtattcgtga
     9421 atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg gcgctttgac
     9481 ggtaggatta ggttttaaaa
//
LOCUS       DAJSVD010000103         9428 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSVD010000103 DAJSVD010000000
VERSION     DAJSVD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.105"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..535
                     /locus_tag="ON498_002282"
     CDS             <1..535
                     /locus_tag="ON498_002282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940514.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKIRPFIF"
     gene            562..2373
                     /locus_tag="ON498_002283"
     CDS             562..2373
                     /locus_tag="ON498_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940515.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2434..3903
                     /locus_tag="ON498_002284"
     CDS             2434..3903
                     /locus_tag="ON498_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3940516.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3914..5923
                     /gene="topB"
                     /locus_tag="ON498_002285"
     CDS             3914..5923
                     /gene="topB"
                     /locus_tag="ON498_002285"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3940517.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6039..6188
                     /locus_tag="ON498_002286"
     CDS             6039..6188
                     /locus_tag="ON498_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940518.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6269..6865
                     /locus_tag="ON498_002287"
     CDS             6269..6865
                     /locus_tag="ON498_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3940519.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6878..7777
                     /locus_tag="ON498_002288"
     CDS             6878..7777
                     /locus_tag="ON498_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3940520.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7780..7911
                     /gene="ssb"
                     /locus_tag="ON498_002289"
     CDS             7780..7911
                     /gene="ssb"
                     /locus_tag="ON498_002289"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3940521.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7933..8265
                     /gene="ssb"
                     /locus_tag="ON498_002290"
     CDS             7933..8265
                     /gene="ssb"
                     /locus_tag="ON498_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3940522.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8287..8544
                     /locus_tag="ON498_002291"
     CDS             8287..8544
                     /locus_tag="ON498_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940523.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8703..8825)
                     /locus_tag="ON498_002292"
     CDS             complement(8703..8825)
                     /locus_tag="ON498_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3940524.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9015..9239
                     /locus_tag="ON498_002293"
     CDS             9015..9239
                     /locus_tag="ON498_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940525.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
BASE COUNT         3505 a         1601 c         2008 g         2314 t
ORIGIN      
        1 catggcagaa gaaaaagaac cactagacaa tagtcgtttg ggaaaatcca aaagaaaatt
       61 agtacgattg caaaatgaat taaacgaaca aattgaaaag atgtttgaac accagagaaa
      121 gaccaacggt caaccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      181 gcgaatcgaa aataaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaagt
      241 agaaaaactt gaaagacaag aagagataaa ggaaatgggc tataacaagt acggcggact
      301 ggatatgacg atcgagaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      361 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      421 acagctgaaa gaaagatcgg aaaaaggaaa agaaaacctt cttcctgaag tacaagcaat
      481 cattgacagc ggacgagtga cgcaatggaa aaaaatccga ccatttattt tctgaaaggc
      541 tatcgaaaag tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca
      661 actgattttt tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atactcattc tcctgaagac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa tagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaacttacta cacgattgaa
     1441 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     1501 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     1561 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     1621 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     1681 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     1741 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     1801 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtt ggagaaagct
     1861 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     1921 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     1981 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2041 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc tgttggttca
     2101 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2161 ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt aaaaggaaaa
     2221 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaattca
     2281 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2341 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2401 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     2461 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     2521 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     2581 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     2641 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     2701 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga gacagaaaag
     2761 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     2821 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgct
     2881 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
     2941 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
     3001 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
     3061 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
     3121 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
     3181 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
     3241 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
     3301 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
     3361 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
     3421 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
     3481 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
     3541 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
     3601 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
     3661 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
     3721 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
     3781 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
     3841 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     3901 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     3961 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     4021 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     4081 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     4141 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     4201 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     4261 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     4321 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     4381 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     4441 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     4501 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     4561 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     4621 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     4681 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     4741 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     4801 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     4861 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     4921 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     4981 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     5041 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     5101 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     5161 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     5221 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     5281 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     5341 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     5401 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     5461 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     5521 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     5581 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     5641 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     5701 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     5761 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     5821 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     5881 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     5941 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     6001 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     6061 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     6121 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     6181 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     6241 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     6301 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     6361 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     6421 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     6481 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     6541 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     6601 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     6661 tgggaatcct attatagctt atgatccaga aagaacttta tgtgacattt ggaatccctg
     6721 gtataccgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactatatgg agtcggacag
     6781 aaaaaatctt agaaaattga atgagtatcg acgaatttta ccaacagaca aaacgatgcg
     6841 ttcttatatt atggctttaa attaaaggag aagatacatg acacctacgc agttaaaagc
     6901 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     6961 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     7021 aaaaggcggc ttcttgattg gctcaaagta tggcattgaa aatcgcacaa ccaaagatat
     7081 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttaacaacgg ttctaaacga
     7141 aattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     7201 gagagaagcc gattactatc caggatttag cttacgtgtg ctggcacatt tggaaaacat
     7261 gcggccagat tttaaggtag atgtgactac aggtgactcg atctatcctg caacgatcac
     7321 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     7381 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     7441 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     7501 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     7561 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     7621 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     7681 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     7741 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     7801 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     7861 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     7921 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     7981 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     8041 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     8101 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     8161 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     8221 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     8281 gtaagcatgg caaaaaaaat tagtctgacg atggatctat tgatacccgt aattgcattt
     8341 atagacggga tttctttggg cgtcactgaa aatcctttgg taaatagaat tgcgaaagga
     8401 tcactatttc ttgtattcgt gtatcttgtc tgttcttatc ttttaaaaag agcaggaaag
     8461 gataattcct tttggcgatt gactaaagtc acagtagcag cgacagctgt tatacttacg
     8521 gctattctac cgctttatat gtagagttaa cgggaaatcg ttgaacttct tgcgaaatat
     8581 tgctataatg aacatgaaaa agaggagttg cgctaacaac ccctcattaa cagcaccgat
     8641 taaagaacgg tggcagaata ttagttattt ggaaataacc gctgacctgc taaagtagac
     8701 ggttatttct ttttgtcatt tttaagcata tttacaatca aactaatcaa agcaatggta
     8761 aacataccaa aaccaagaat ggtttgaatt gtctcatatg cggacaaaag gcttactcct
     8821 ttcatagatt ttagcactta cattcataag caccaccacc tttccagaag tcgccaccgt
     8881 cttcaaactt tactgtcttg actattatag catatctaaa atcgaataaa aattctcaat
     8941 tttagctgtt tttgctataa ttttagcgag gtgatcgtga tggaacaata tattggttta
     9001 ctcgataaat tagggtgatc aagacacagc cattgcttgt gcgatttaca ttacgcacga
     9061 gtcatgaacc aatcaattgt gtagtggcca atatagaaat agcggataaa ttgctgattt
     9121 tggaagaagg caagtatact atttcagtga caggtcactt caataaacga aatcaactag
     9181 tcattgaatc aatgcaaatt agaaaccctg atcattttac gagattaatg ggcatataaa
     9241 taaaagaaga gctgcagtcc taaatgagga ttgcagctct ttttgtttta catagaaaca
     9301 agaaaaatga aaaggccttt ttaggaaggc gcaagcgcct tctcaattga aaaacctctc
     9361 tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgttgc acgttcacta
     9421 agataagg
//
LOCUS       DAJSVD010000104         9343 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSVD010000104 DAJSVD010000000
VERSION     DAJSVD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.106"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(269..3910)
                     /locus_tag="ON498_002294"
     CDS             complement(269..3910)
                     /locus_tag="ON498_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940526.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPAPDN
                     QENNQETVINKSVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGELLKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(3913..5862)
                     /locus_tag="ON498_002295"
     CDS             complement(3913..5862)
                     /locus_tag="ON498_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3940527.1"
                     /translation="MENSTEFFKNIQNEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(5904..6962)
                     /locus_tag="ON498_002296"
     CDS             complement(5904..6962)
                     /locus_tag="ON498_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR3940528.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKEINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(7081..7536)
                     /locus_tag="ON498_002297"
     CDS             complement(7081..7536)
                     /locus_tag="ON498_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940529.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEDIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(8120..8923)
                     /locus_tag="ON498_002298"
     CDS             complement(8120..8923)
                     /locus_tag="ON498_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3940530.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         2700 a         1930 c         1336 g         3377 t
ORIGIN      
        1 taaattttcg atgattaaaa gagattttcc attaaattat ttcacttttt ttattggcta
       61 aaaatgctgg tttatcaagt ttttgtttat ataataattc tgttctttac cagcataagc
      121 cctttttaat tatacaactt tgaacagtaa atatttaatt catttataga caatttaaaa
      181 taattttttt gcataaatag aaattttcct tgacatcttc agttagaaat caaagtaaaa
      241 agaaggagca aatcaatgct ccttcttttt actcgtcttc agacttattt tcagttgatt
      301 tttgattacc gtattttaca atatagtcct tgactgacat ggtagatttc ttcactactt
      361 taccagtttt ggatgctcct gtagcagtag ccttggcaac cttagccgtt cctttaccta
      421 ttttaatagc ttcgttttta gcttcatagg ttatccctgc tagactacct tttggagctt
      481 ttgctaaagc tttatctgct gaggtgtaaa catttttacc agtttttgat agatattcaa
      541 cggctgattt agctgttgat ggtttcttgg ttgattgatt agatttccct gaattatctg
      601 aagtagattt agaattattt gttctattat ttttatttgt cagttcaggt tctctaattg
      661 attgattgtc tttcgacatt cctgatccaa tatttgtttc atatttatct gtttgattaa
      721 cattaggagt aggaatgaac tttttcttca catctgattg ttctttatcc gtttcttttg
      781 ttagcgattc tctgtcttta agtgctgtgt cattccgaat agcactatta tcaaagttgc
      841 tgttaagctc tcttgactga ctttcaccag atgctccatc tcctaattcc cgatgcactc
      901 tgtctgacac gtcctcacca ctcatttcat tcctcagttc ttgatgagtt tgatctaaag
      961 taatctcgtc atcttcattc tcaggaattg caacagcaat tggcgtatgt cgttgttgtt
     1021 cgagttctct atttcgatct gccacaagcg catctctcgt ctcaatgata ttcccatcct
     1081 catcgactaa atctccattc acattatatc catcgctgtg tttaccaagt ttattaccaa
     1141 actcatcaac aagatttcca tcttcatcaa ctaaacgccc tttttcgtca actaaacgtc
     1201 cgttactatc ctgaccaaaa tcaccattta gcaacctgcg tccattttcg tcaacaagat
     1261 ttccatcttc atcgactaag cgaccttttt catcaattaa acgtccttcg ctatccatac
     1321 caatattacc atcttcacta tcttccaatg gtttaccatt agtatctata aggtttccat
     1381 cttcatcgac caaacgccct ttttcgtcaa taaagttgcc tttagcatct ttaccgtatt
     1441 tactatcttt taataattca ccattttcat cgacaaagtt tccgttttca tcaaccatac
     1501 gtcctaaatg atcaatatat tgttgggagt tattacgtag ttgatcttga cgaactaaat
     1561 ctttgaaaga ggtattattg attgttgaac caactccgcc accgttgaat gacattcctt
     1621 ctggtgtttc aggaattact ttatcagcaa tggacttagc atcagcattg ctatctccca
     1681 tattgttcaa acgttcagcc attttttgcg ctccaactgc tccaagacct cctgcaacca
     1741 tatcttttac tgagctgttg ccaccatcat aatagggatt tttagctcct tggcttttct
     1801 tgattgcttt agaaacgccg tccacaactc gcacagcatc atttctatta ccaaataata
     1861 acttagcacg ttcatcaaac ccaaacgcta cttcaagtgg taagccaata agcatattaa
     1921 tcaaattatt tactttttcg ccttttttaa agactaaaag atatcctaca cctaaaatca
     1981 tagcagatcg aacaatattt gctacgccat cgccaactaa tgtattagga atgaatccca
     2041 cagcagtatc accaaatgca cgaataaaag taatcagaaa gataggtaat tgtagtccga
     2101 tcagagatgt tgtaacaaca cctaatgaaa ttgccatcac acctgctcct gatgcaagtg
     2161 atcctgttag aactacttcc caaatccgaa taaattcacg tttaaatgct ttcacgatat
     2221 tcacattgaa catcgcccaa gcacatgcta agtaaagaac tgctaaaacg attgattcag
     2281 cgttcatcgc tagccacgag ccaaacttac ccatcgctcc atgaccaact aaggatgctg
     2341 tacgccattc tgtttttact gttgacatat tcttagcact gccttgaatt cctgaaggag
     2401 cgatattata cgcataaaac ttagctcctg atgtatcaag tgccgattgg agcaataaag
     2461 ctaccgactg tgtgctaaaa gttgcattgt tagcaacgcc ccaaggttga gtagcatcaa
     2521 agttatgcgc tgttcctttt ccatcaggct tgcgattgtc gtctgtcaca ttttgatttg
     2581 ctgaccaaat atatccattt aaattggttg gtttcttaat tgcacttcct tcttttccat
     2641 cccctaaatt atagcctgtc caaacagcag gcgctcttcc catataagca tcattttcgg
     2701 cgacattaac atcaccggca taagtattaa tattgaaagt atcattcgat gaccattctt
     2761 ttaatagagc cataccgact gaattagaat ctagatcatt attttgtctg gcataactgc
     2821 tagtattgat cgctcttatc attgcagatt ttttagatgg ttcataatct gtatcaacga
     2881 acttgatttc ggatgtaggc tttccgtcag gtacatcacc accagtatca gatgatggag
     2941 caagattttg agtagaagcc caaagtcttg tgttaatgat gtagtcagaa acaacatttg
     3001 ttggtatatc tccatctttt tttgctgact ggatgccgtt gatataaact cccaatagcg
     3061 atcctgccac tggtaagaat ataaacaata caaaaatacg aacaatccat ttttcaagta
     3121 ctggtgataa tcccttcgag ttaacaccat ctttcgacaa taatttcatt ccttgataac
     3181 ctagtattgc tgtgaaaaag agcatagata agcttgctat cgctttaaat aattcattat
     3241 ttaatcccaa attgttaagc aagctagtta atgctttaga taccggatta gatacatccg
     3301 atccaccttc tccccacccc ataagagaaa atatattcat actcatgaaa aattttccaa
     3361 ggagttcatt tatggattca gcaatattag aaaggattaa actaattgat ccgataaaac
     3421 taccaatcgt cctaccaata cttcctagaa tggatgatgc ttgactggca atagcatttc
     3481 ctgagactgt ttcaatatat ccatagtaac tataagttgc tagagtagat gctagttgtg
     3541 tacctgcatt cccccctgca atcgaattac cttgtttaga aatatcagaa taaggaaaat
     3601 cagcactgat accaccttgt cctaacaaat tcatttgtcc agttttagca gcactatcat
     3661 tgacttcttt atctcccatc aagatacgag catatagtgt ccacatccct tgttctgccg
     3721 gtttactttc cgcataagca acgctcttat ttataactgt ttcttgattg ttttcttgat
     3781 tgtcaggagc tggatcattc attccaaatg gagcgcctaa agttactgct aatacccctc
     3841 caataactag acctcttacg acccatcgtt ttattctaag gttttgtagt aaatatttct
     3901 tgaatttcat agctacgccc ctccaatcga aaatacaatc ttgttttctt tactttcagc
     3961 aacggaaggc tttgcaataa tttcagcatt agaatcacct tttccttcaa acgctccagc
     4021 ttcactcaac ttaacatcga atatttttgc gtttggataa gtaattattg cttcagtaat
     4081 cgcttcttca aactcagatt caactaaatg caagatattc cttgcacccc ctgatttaga
     4141 atcctcttgt gaagccatgt caaaaactaa gtgcatggag agatcgtctg cataaaaatt
     4201 ttgaccatat actctttctt tcctaggtgg aagacgtaag tctatattta aatgttttcg
     4261 tactaattca gcactcttca acatttcatt ataagcaatt tccagaagag tttgttcgga
     4321 taaagcccaa aatggaacta ttcgtccaaa tcggttaata acttcaggct ttagaccttc
     4381 accaatcaag gcttgtttga tccctgatgc gacttttcgg tattctcttt ttagttcagt
     4441 gctgtattca ttagaatttt ccttgtttgt aacacgagat agtgagctat atcggtttaa
     4501 cgaatcgaac atctccgcac ctgcatttgt tgtcgcaaat aaaattgtat ttgacaatga
     4561 aacggtgtcg tgaacttctc gatcatccca tcctctttca gtaaaactta tcctaccttc
     4621 atctaaaatt gataaaaagg catcgagtgt ttctacacca gctttctcat attcatcaca
     4681 cacaataatc gcagaaggat catgccttac atgagtacct atgagtcgtc taaaacgcat
     4741 tgcatcatct ttggttttgt aatccggcat attaactaag agaactttat tttcatcgcc
     4801 ataaagtcct cttgccacgg ctttagccat tgcagtttta ccgacccctg ttgatcctgt
     4861 aaacagagca gttgcttttg gttgctttcg acctttaaaa cgatcaaatc caagcttcat
     4921 acgtttaaac aacattgtta tagtataaac tgctaacggt tgccctttta tggactttaa
     4981 ttcctcttga agtttttcca tatcaaaatc agaatctagc tgaatattat acttaccaaa
     5041 tatttgcaat agactttctc gattataagc atttccgtct actcgatctt ttgcaatcaa
     5101 atcctccatt acggcaattg atttagccgg ttcggacaaa tcctcacgat aaagcttgtt
     5161 tgcttgtacg atttcttgaa gtaattcatc aggaatcctt tgcgataatt taaaagaagc
     5221 ctgaggatca tctttaacag aggatgtatt tagccattgt ctcaaaatat cgacgacaac
     5281 atcgcttttt agttcattca attctaacgg ttgtaaccgt ctccctaagg catcatctct
     5341 tttgatatag ttttcatatt cttttcttgt agtagcggta atcaattgaa gtgattcatt
     5401 tgctaacggt tcttttagcg aatcaccccc aattttattg ccactcccaa aagaactaac
     5461 aactttatga aactcgtcaa tgaagaggac aaattcattt tctttgtcct cagattttaa
     5521 aattcgctca tattctttca atcttggaat taatgtttct aaacgtgttt ggaggacatc
     5581 atctccccca acacgcaatt gtccgattga taatttgtat acttgtactt tctttccatc
     5641 ttgtcgctgt ctttcagccc attcacttgc aagtgctgtt ttacctgttc cggcttcacc
     5701 taacaagata gcttgcggtg tttccggtct attcaagatc agtgacaact catggagttc
     5761 gtcgtcacgt cccactaact tatgtttcgg tggtttcatt ggtgtgagag catgattaaa
     5821 ttcattttga atattcttaa aaaactcggt actattttcc atctgtttgt gttcacttcc
     5881 tctaaaataa gaataattta cttctagtca gtcagagtta ggtacttaaa tgtcgtcttc
     5941 atgtctccaa aagaatcttt gttaaagtca gcatttttgt attgatcaat caatttttgt
     6001 gtatcattga tttctttaat ttcggtctcc attcgactgt ctagttgacg aatactttct
     6061 tgcgaatctt tatcgtcagg attttgttgt aaacgttcca gatactcagc ttttgtggct
     6121 tcaattttct tgatgttagt atttgaagca tttaactgat cttctagctt atttacaaca
     6181 ttattcttac ttgtaactgc tacaactttt gaataatcaa tatcgccatc tttggtaaga
     6241 aagcttcctt gatagacatt agttgaatct gaatatggat ttattctgaa attgatatta
     6301 tcaggagtat taacaggttt tgcttttttc ccaaaagaaa ttactccttc attattaaca
     6361 tcattcatag aatattgaga aagtgctttt gtaactgaat ctgcttcatt cacattgact
     6421 ttggtatcat cgtcagctgt atcatcacca gaaattagtg atacttggtt tgcaataaaa
     6481 acttgataag ctctttcatc taatttacct tttaggaata agaaagcatc cccatcagta
     6541 cttaacatcc catagctcat ttcaagcttt ttaggacgat ctttttcttt cgttgccata
     6601 tataagttgt agttttttcc ttgcgtagat aacaactttc tagcgttttg cgagtaaccg
     6661 agttttacta ctgttacatc tctgttttga tctgtccaca catctgaaat agtgatctct
     6721 gctcctgatc gagcaaactc caatgaagag cctataggtg ttgtattgtt cacgagataa
     6781 taattatagt ttcctaattg ataaatgtag ctacccaagc ctgttaataa aaaaagcact
     6841 cctagaaaag ttgcaagcat aatgcgtaat ccgtgacgtg ttctcttatc cttatcacca
     6901 agcaaccctc caataatact gtattgatct ttggtagatg ttgaattgac gtttttttcc
     6961 attagtttgt ctgccccctt tcataaaaga gcaaacaatc agacgttttt tcggtaattc
     7021 taggttcggg attttgaatc atttcggtat attcttcccc attaatattt aacacaaaaa
     7081 ttatcttcct ttcttcgttc ctttcttttg ctctttattt tccaaataat caatgatttt
     7141 tgctattgta ccaagatagt aattcccctc ggctttgttt tttcggattt tgctaaccat
     7201 tggagtaggt acaatatctt catctctcaa ttcactgaaa gattttttac ctagatattt
     7261 tccaataaca gattttaaaa aacgttcaaa atctttctcg taagttgttt tcttgccttt
     7321 tagttcatgg ttcatattgt caacgacttt tgcggtttca ataattgttt tagttctcag
     7381 cttaataatt tcatctggaa ttttcaatcc tcgtaatttc atcgttgtac taactggtaa
     7441 cccatgcttt actgcatcag caatactaaa actttttaga tcatctagta agctctccat
     7501 atagttaaca aattcatgcc aattaatagc attcattgtc tcaccccttc atttttttaa
     7561 aatgaggttc gggcgtaaca catttatgtt attataatat cataataaca taaataaatc
     7621 aatcagcaaa tttagatatc ccttaaagta tcagcaatta ttcaacaaga tatttatttt
     7681 agctatttca gtcatcatga aagactaatt agcaataaaa taactaatat tatccattta
     7741 tcagacgaaa ataaaaaact tatctaatta ctctatatta acttttaaca aatagttgac
     7801 ttgtccaaaa atgttacctt caatctcaca aagatattaa gatgtttcct accaaaactg
     7861 atatcaccgg actttttatt ctgtgttgtc caaaagtgtt acctttagtc gatcaacaaa
     7921 ttaaaaaaaa ttgttttttt cttgacttgt ccaaaagtgt tacccatagt tgttaaaatt
     7981 tgtatagatt agccattctt tcaaatatta tttagactga aacttgctta caaaaaaact
     8041 tcttgcattt attgtccaaa agtgttaccc atactttttt aaagaagcag gttgtattaa
     8101 aattacaacc tgcttcttct caatcttctt ctaaccaatt atgcattgaa atggttggca
     8161 agtttgattc tggctcacta aaagtgaatc tgagcctatc aactcttctt cctttctttg
     8221 catagatttt ttcataatca aaagtttcaa atacctgtat tccgttttca tcaggagcta
     8281 aaaattcttc tttcgcctgc tctagtattc ttttgtcaat atttgtcatt ctataacttg
     8341 aaggtatact taatgctctt ctaaaatctt caactttgac tacccaataa ccatttcgcc
     8401 actctttatt tctgtattgc atgagatgac gatacaattc ttttgtgtag ctacttctta
     8461 gttttgtaaa attatcaagt tcaaaacgag taaagttttt gccaattgag tttaagataa
     8521 attcaaattc ctcatttgcc ttaattgtaa ctgtttcttg ctgtttatct atccggaatt
     8581 tttgaaaaag ccacagctgt tcatagacgt ctgaatcttc aaaacggtag gttaaagtac
     8641 ctagtttgtc actcaaagct tttaacacag aataaaaact gtccttttct cttctgtcgt
     8701 atttgaccaa ataacgaaag aggttgaaat caaaagttac ttctgctgtt ccttgacgtt
     8761 tcatttttga taaaatagcc caaaacacat ccatttcaac aggcttaaat cctcgcaaag
     8821 ctgttgtatt cagaccattg ttataacgca caattgcatt tgtttgttct gatactaaat
     8881 ctacatcact atcaataatc ttttcggtaa cttcttgaga cattgattac tctccaatct
     8941 tctttttgcg ctcctttaat catgttaatc tggtactaat taaaggaaac aattttttct
     9001 gcttgttacc taaattaaat cacaactaaa ggtaacagtc aacaaagatt tgttactaat
     9061 ctaattttct atttttttgc cttccaaatc aagaaaaccg aaggtaacgt cttatcctag
     9121 tgtgatacct caaatgtcca cttttgttac ctttgctgtc caaaactgtt acccttactg
     9181 tccacttttg ttacctttac tgtccaaaag tgttaccttt ataccccttt aatacttact
     9241 ctcccaagag attaagcctc ccctaacaga tatattaaca gatatttaac agatatatta
     9301 acagatattt aataaaggag gattactcct ctcttatttc aaa
//
LOCUS       DAJSVD010000105         9103 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSVD010000105 DAJSVD010000000
VERSION     DAJSVD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.107"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            72..2681
                     /gene="clpB"
                     /locus_tag="ON498_002299"
     CDS             72..2681
                     /gene="clpB"
                     /locus_tag="ON498_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3940531.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2832..3518)
                     /locus_tag="ON498_002300"
     CDS             complement(2832..3518)
                     /locus_tag="ON498_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940532.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3496..3990)
                     /locus_tag="ON498_002301"
     CDS             complement(3496..3990)
                     /locus_tag="ON498_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3940533.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4010..5230)
                     /locus_tag="ON498_002302"
     CDS             complement(4010..5230)
                     /locus_tag="ON498_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940534.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5377..7212
                     /gene="lepA"
                     /locus_tag="ON498_002303"
     CDS             5377..7212
                     /gene="lepA"
                     /locus_tag="ON498_002303"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3940535.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7408..8319
                     /locus_tag="ON498_002304"
     CDS             7408..8319
                     /locus_tag="ON498_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940536.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         3088 a         1596 c         1849 g         2570 t
ORIGIN      
        1 agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt taattaggag
       61 ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg attgctgaag
      121 ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat gtatggaaaa
      181 tctttttaca acctaatcat tttggtcgaa atttctatac ggatgccgga ttggatgtcg
      241 aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt tctggtggaa
      301 acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat gaagcagacc
      361 aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt ttagcgttga
      421 tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta tctgaaaaag
      481 aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca caaaaccaag
      541 aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg
      601 gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt
      661 cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg aaaacggcaa
      721 tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata
      781 aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat
      841 ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct
      901 tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga agtatggatg
      961 ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc ggtgccacaa
     1021 ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt cgctttcaaa
     1081 aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt ggattaaaag
     1141 aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta gcagcagcta
     1201 ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc gatttgatcg
     1261 atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa ttagatcaag
     1321 ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg
     1381 atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt cgtgaagaag
     1441 caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct gtttcagcaa
     1501 aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat aactatgatt
     1561 tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa gaattgaagg
     1621 aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa
     1681 atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag
     1741 gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg attggtcaag
     1801 atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga ttgcaagacc
     1861 caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga aaaaccgaat
     1921 tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta cggatcgata
     1981 tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct ccaggctatg
     2041 taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct tatacaatcg
     2101 tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc
     2161 tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa aatacggttc
     2221 tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact gctgatggaa
     2281 cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac ttcaaaccag
     2341 aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta gacaacgtga
     2401 aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat caagaaatcc
     2461 tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat gaaccagcat
     2521 atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca ctagctaaag
     2581 aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta cttgatggac
     2641 aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt gagactataa
     2701 aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat aaacagtgtt
     2761 ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg cttctgtcac
     2821 aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa gtaacgctca
     2881 tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat cgtatttgtc
     2941 agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt gccatcagaa
     3001 gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc ccagaactcc
     3061 caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa ccaaggactg
     3121 gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc aagagctgtc
     3181 aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat actgatcaaa
     3241 tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa caaaacgatt
     3301 gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa aatcaatgca
     3361 atccctaata aagattccac gataaatgtg attccttgag aagtcgaata tttttgtgtg
     3421 ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa agctatttgc
     3481 acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt ttacttctga
     3541 aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac ctattgtata
     3601 taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga tttttttcgg
     3661 gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga tgcctttcgc
     3721 ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat ctagtaaagc
     3781 aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca ttcgactgtc
     3841 tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga tcgaaagctc
     3901 agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat cacttttagc
     3961 aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat caaaaataat
     4021 cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt gtttcttcat
     4081 ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc atcatcaagc
     4141 aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt aagacacgga
     4201 cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga gcgacaggat
     4261 cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt
     4321 cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg atctgactat
     4381 cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc cagtagatca
     4441 tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga tcaagataga
     4501 acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca cctcttggtt
     4561 catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg aaacgccaat
     4621 actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg gcaaatttgt
     4681 cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata ccacttactg
     4741 ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca gacatgattt
     4801 cccatccttt acgccgatag taggggatac tgtaaggata caaataagag atccattgct
     4861 tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat ccatggttag
     4921 catattctgg ataagttcca actccagtca caccacccat tttataacgt gcaccgtgaa
     4981 tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg tggaaccatc
     5041 cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct cgtttattct
     5101 caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa agcaattcat
     5161 tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct ctaaaatctt
     5221 tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa aattctacaa
     5281 aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt cgttggtata
     5341 atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa aatgaaagaa
     5401 cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg gaaatctaca
     5461 ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag
     5521 ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta
     5581 gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat tgacacccca
     5641 gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt
     5701 ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt ttatttagcg
     5761 ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc cgcagcagat
     5821 cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag tgaagctgta
     5881 ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat cgtggaatat
     5941 gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt tgactctatt
     6001 tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat ggtcaaaccc
     6061 ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga ggtcggggtc
     6121 ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca
     6181 gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt agcagacaat
     6241 cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta ttgtggtctt
     6301 tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa actgcaattg
     6361 aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt tgggttccgc
     6421 tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga acgggaattc
     6481 aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa aacggatggt
     6541 acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat tcaggatgta
     6601 gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg tgcagtaatg
     6661 gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga tgattaccga
     6721 gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt cgacaaattg
     6781 aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc
     6841 aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt aagctttatc
     6901 gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta
     6961 atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc
     7021 cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt
     7081 tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat gaaacaaatc
     7141 ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga tgagcaagat
     7201 agtgtgaaat aacttataat caatcgaaaa acctattaaa taaacctttt tgagtagatg
     7261 caacactgag gattatctgt ataaaagatt ttaaactgtc atttggttat cacggcgata
     7321 ctaaaattaa caaaattgga atatttttct tgaagatttt tagctgtcag tatatgatga
     7381 aagcatctca taggaggtgc ttttttcatg gttgataatt caagtggaag ggttcttaaa
     7441 tctctaagaa aagagaaaaa actatctcaa aaaaagttag ctgatttagc aggtatttca
     7501 caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa tccctaaaga cgtagataat
     7561 actctaagca agattctgaa tatagaaact ctaataaaag atgaggaaga taagattgag
     7621 attctcatcg gtaaactaat tgcatacaga gatatgaata agttacttaa caaagagtta
     7681 gctgataata taggaattag tgaagtatta ctcagttatg tgttaaatag aaagagaaat
     7741 ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat taagtaatga aaaagaaatt
     7801 ttgaaggaga ttaatcgtga tagtgagata tttagtttat caaaagacga taaaattgtt
     7861 atgggaaaaa gaatacgaga agtccgaaag aaccgggaag agacgcttga aaaatttggt
     7921 aaaaacttta ccatctatac aggcaaaaat gttataagta gatgggaaaa aggtattaat
     7981 attccagata ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac tgttccttac
     8041 cttatgtacg gagaagatta taagaatata cttccaaaag atgaaagagt tagcgatttt
     8101 aaaaagataa attcttttag tatgggatta aggatgagga aaattaggaa agattattat
     8161 ctagaaaggg aggagtttgg aaagcttttt tctccttcaa tttcaaaatg gtccatagat
     8221 aggtatgaga atggtagaga tatacccaat actaatcgaa ttatccaata tgcttatata
     8281 ggaaatcttt ctttagaatt tttgatttac gggatataaa aatagtttac gtcgatactt
     8341 attaagttat cgacgttttt tattatagaa aaaatttata aaaggaggtt tcttgatatg
     8401 ataagaaaca aaaagggttt gttgaaagtt gctttactgg cagctttttt ctaagtaact
     8461 ttttgacaag ctcaatggca acacaagcgg aagaagtaat acaagaacta actgttatga
     8521 ataacagcta gataaaatgg gctatgtatg ttatccaaca atttagtaat cactagaagc
     8581 ctagcgaaag ttcgcttgat aagttataga tgagtattga tttgtagtgt ttccaaatca
     8641 aaagggtaca gagccacatg aaatatgttg tcctctgcag cgtcaggatg ggaatccata
     8701 ccatggagat tcggagatct gtgagggaat acttacgtgg ttaatatgtc agttgtgttg
     8761 gacgtagtaa tggtcatagt gggtagattg actgaattct tatatgagaa aatgaaacca
     8821 acaagaagat agggttattt atacagaatt ggggatcttt tctggaaaag taatcggata
     8881 atgcgagatt cgtacggtct tttttaaatt tctctaaaaa aaacggcatc gaatgggttg
     8941 ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt ctatttatct
     9001 ttttacatct atcgtttttt caagtagccg caaattatat cagcattcaa caatttttgt
     9061 atacaagttc tagtaaaagg atctgaagaa aaagttggtt gtt
//
LOCUS       DAJSVD010000106         8988 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSVD010000106 DAJSVD010000000
VERSION     DAJSVD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.108"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            22..95
                     /locus_tag="ON498_002305"
     tRNA            22..95
                     /locus_tag="ON498_002305"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Ile,seq:cat)
     gene            complement(480..977)
                     /locus_tag="ON498_002306"
     CDS             complement(480..977)
                     /locus_tag="ON498_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3940537.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1017..1826)
                     /locus_tag="ON498_002307"
     CDS             complement(1017..1826)
                     /locus_tag="ON498_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3940538.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2160..3017
                     /locus_tag="ON498_002308"
     CDS             2160..3017
                     /locus_tag="ON498_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3940539.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            3010..3912
                     /locus_tag="ON498_002309"
     CDS             3010..3912
                     /locus_tag="ON498_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3940540.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3945..4295
                     /locus_tag="ON498_002310"
     CDS             3945..4295
                     /locus_tag="ON498_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3940541.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4344..5267
                     /locus_tag="ON498_002311"
     CDS             4344..5267
                     /locus_tag="ON498_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3940542.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5345..6238
                     /locus_tag="ON498_002312"
     CDS             5345..6238
                     /locus_tag="ON498_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3940543.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6276..6839
                     /gene="amaP"
                     /locus_tag="ON498_002313"
     CDS             6276..6839
                     /gene="amaP"
                     /locus_tag="ON498_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3940544.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6853..7041
                     /locus_tag="ON498_002314"
     CDS             6853..7041
                     /locus_tag="ON498_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3940545.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7054..7956
                     /locus_tag="ON498_002315"
     CDS             7054..7956
                     /locus_tag="ON498_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3940546.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7988..8230
                     /locus_tag="ON498_002316"
     CDS             7988..8230
                     /locus_tag="ON498_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3940547.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8486..8857)
                     /locus_tag="ON498_002317"
     CDS             complement(8486..8857)
                     /locus_tag="ON498_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940548.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3128 a         1553 c         1742 g         2565 t
ORIGIN      
        1 aaaataaatt ttatttatga tgtaccttta gctcagttgg ttagagcaga cggctcataa
       61 ccgtccggtc gtaggttcga gtcctacaag gtacataaga ggttgtgaaa aagctgccct
      121 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttac ttggctgttc
      181 ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg aaatcagcgt
      241 tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
      301 aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg tgagcctgcc
      361 gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac gtaggactaa
      421 aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga tagatatttt
      481 tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt
      541 tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa agcactctgc
      601 aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac ttcgatctct
      661 ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt ttcaggtgga
      721 atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag aagagggatc
      781 aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat atcccgtgtg
      841 aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat acgttctgcc
      901 aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg attttgcata
      961 tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta ttctttttaa
     1021 tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt tacttggttt
     1081 gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc
     1141 aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga ttcgattcca
     1201 aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg cgtgatttct
     1261 aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc tctttgtagc
     1321 ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct aagagaagga
     1381 tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa atgttcataa
     1441 taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc ttgggtttct
     1501 cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt tctcagtgta
     1561 tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt catttcagca
     1621 atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga aaaaatatct
     1681 ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt ttcatcaaat
     1741 ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag caaggtaccg
     1801 tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac atatctattt
     1861 tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg ctttgtcatt
     1921 atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta gtttttggtc
     1981 aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt
     2041 gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa
     2101 tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga
     2161 tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt
     2221 acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat
     2281 tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa
     2341 ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag
     2401 acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact
     2461 tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag
     2521 acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg
     2581 atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca
     2641 attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa
     2701 ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga
     2761 atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc
     2821 tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa
     2881 atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat acgttgaaag
     2941 atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag
     3001 gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta
     3061 ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt
     3121 cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca
     3181 gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt
     3241 acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg
     3301 gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct
     3361 ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt
     3421 gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt
     3481 tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg
     3541 caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat
     3601 gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa
     3661 ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa
     3721 gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc gtggagcaag
     3781 gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt
     3841 tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta
     3901 gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa attacaatgt
     3961 ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg
     4021 cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct
     4081 tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc
     4141 cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca
     4201 tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt
     4261 ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga
     4321 ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa
     4381 aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg
     4441 aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc
     4501 ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc
     4561 tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg
     4621 ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa
     4681 agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt
     4741 acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag
     4801 cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca
     4861 tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt
     4921 ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac
     4981 agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc
     5041 tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg
     5101 cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat
     5161 taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag
     5221 cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa
     5281 aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac
     5341 agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc
     5401 taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg
     5461 agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg
     5521 agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc
     5581 tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt
     5641 tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga
     5701 aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg
     5761 acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt
     5821 tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc
     5881 aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt
     5941 agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct
     6001 tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga caccattaca
     6061 actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga
     6121 acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc
     6181 aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga
     6241 agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg
     6301 gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa
     6361 cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg
     6421 tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac
     6481 ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg
     6541 agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg
     6601 aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg
     6661 taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag
     6721 aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga
     6781 aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg
     6841 gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta
     6901 cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc
     6961 ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta
     7021 gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa aaataataac
     7081 aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac accaaaccaa
     7141 tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca tccagatcca
     7201 gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga aaagcatgca
     7261 cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc aatcgatcct
     7321 tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca agaaaaactt
     7381 agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa taaccaagga
     7441 aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga agataaagtt
     7501 gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc tgcaaacggt
     7561 ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac ggctgggatc
     7621 gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt ggaatatggc
     7681 aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat
     7741 atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa
     7801 gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc
     7861 ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag cggcgcacaa
     7921 aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac attaggagga
     7981 acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag caattgcagg
     8041 agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag caggtttagt
     8101 tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg caggaatggc
     8161 aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat tctttgctcg
     8221 aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata
     8281 agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat attatcgaag
     8341 atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac ttttgtcaca
     8401 gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc cttaaatgtg
     8461 tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat tttcttgaga
     8521 taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg atccacaaat
     8581 ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag agattgaaaa
     8641 agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa taatagctcg
     8701 taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca gcaatcgtat
     8761 catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga atatagatca
     8821 cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta attgtgtgag
     8881 ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt cgtaacaaat
     8941 gttatctaat gtatttttaa ataaataatc attattatca aaataatg
//
LOCUS       DAJSVD010000107         8967 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSVD010000107 DAJSVD010000000
VERSION     DAJSVD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.109"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..177
                     /locus_tag="ON498_002318"
     CDS             <1..177
                     /locus_tag="ON498_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940549.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="ON498_002319"
     CDS             190..690
                     /locus_tag="ON498_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3940550.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ON498_002320"
     CDS             787..2634
                     /locus_tag="ON498_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR3940551.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ON498_002321"
     CDS             2637..3533
                     /locus_tag="ON498_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3940552.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ON498_002322"
     CDS             3583..3972
                     /locus_tag="ON498_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3940553.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..5461
                     /locus_tag="ON498_002323"
                     /pseudo
     CDS             3959..5461
                     /locus_tag="ON498_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6206..8032
                     /gene="ltrA"
                     /locus_tag="ON498_002324"
     CDS             6206..8032
                     /gene="ltrA"
                     /locus_tag="ON498_002324"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3940554.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            8166..>8967
                     /locus_tag="ON498_002325"
     CDS             8166..>8967
                     /locus_tag="ON498_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3940555.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3078 a         1401 c         1939 g         2549 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatttctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac ccttaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 gggtgcgaaa cgttcctact tgaaaagagt aggcacagca cttcattaaa tcaatattta
     5521 atgtgtgttg gagaactaaa cacacgaata gtcgaatgac ggggtaacgc cctgaaaggc
     5581 tactttaata ctctgactgg cagaaacttg agtaaacaag gctctgtcag aagctcggtg
     5641 aagtcggcag acagacaccg taacagataa tgctgacgaa agattcccag aggtgggcgt
     5701 tgtgtcctat atgccggaag tctataaaat atctatggtt agaatgtaga tgagaggtct
     5761 actgacgaat ccttgaatgt acaggtctat atcggggctg gatagaaatg tccagtgaca
     5821 caaacagtgt cgctactgtg ggtgagtaag actccgcatt tatgaaaatc catataacgt
     5881 taaaggcgtt atcaaggtag caggcttata gaggaaacct aaggatatat gtacagatag
     5941 tgtgtttgga acgttgaaag ctgacaacat ggagatttta ctgtctgtga agtgttcaca
     6001 tcagaaagtt attgtgagat tagcaatgat ataaggtgtg ttaatgtcag tgagagtggt
     6061 ggcacagtac ctatgaaacc gtgataacaa gcggtggagg gatagccact agtcatatga
     6121 agaatagaat tactaagaaa aatggtatgg gttcgagtaa gactaagaga tgtacttgct
     6181 cctgcaaaca aggagggtac agaccatgct aaagaaaaac aagctccgct ataacgagta
     6241 ttatgatatg caacatattt atgacgagtt atacgcacag agcaagaacg gtaataactt
     6301 ttataagtta cttgaaatta ttggttcgga acaaaatatt tgtttagctt accgtaatct
     6361 gaaatcaaac agtggcagta aaacggctgg aacggatgga atgacgatag acgatataaa
     6421 acaactcagc aatgctgaga ttgttgctac cgttcgggaa agccttagca actaccgccc
     6481 caaatcagtc agacgtgttt tcattccaaa agctgggagc gataaaatgc gtccattggg
     6541 gattccgtgt atctgggaca ggctggtaca acaatgtatc ctgcaagtcc tagagccgat
     6601 atgcgaacca aagttccata atcattccta tggattccgt gcgaatcgaa gtgcccacca
     6661 tgcagtcagc agagtaacga cactgataaa tttatcaaaa tatcattact gtgttgacgt
     6721 agacattaaa ggatttttcg ataatgtcaa tcatggaaaa ctattaaaac aaatctggac
     6781 acttggtatt cgggataaaa gattgatttg cattatcagc aaaatgctga aagccgaaat
     6841 tgacggcgag ggtgtcccag aaaaaggaac gccacaaggc ggtctgttaa gtccactgct
     6901 ctccctcata gtccttaatg aattagactg gtgggtaagc agtcagtggg aaacattcca
     6961 gccgaagaac cgcagtaaaa atgggtggct tcaatacgca aagaaatata ccaaactgaa
     7021 aagcggtttt attgtacgtt acgctgatga ttttaaaatc atgtgttcaa cctatggaga
     7081 agcacaaaga ttttaccaca gtaccgtaga ttttcttaat aaaaggctga aattagaaat
     7141 cagtccagag aaatcaaagg tagtaaatct gaagaaaaat tcatcagatt ttctggggtt
     7201 caaaatcaaa gtcattccaa aaggaagaac gaaacacggt tatgttgcca aaacagatat
     7261 gaaccaaaaa gcattaaaga aagcgaaaac aaatctaaag ttaaaggtaa aagatatagc
     7321 cagacatacg actggcttca atatcagcag atataatctg acagttatcg gtatgcagaa
     7381 ttactactgc atagcaacta acgtatataa caacctgaca gaagtaagtt acgctctctt
     7441 accgaccata cgaatccgtt taaggaacat tgcaaaatca gttccatttg aaagtacaag
     7501 ttcagaattt cagagcagga caaaaggaat cagaccaaaa acaaaaatag tgatgattgc
     7561 ggataatccg ctattgccga ttcaaggagt acaacacaaa aatcctatga atttctcgca
     7621 agatatttgt aattttacaa agcaagggag aaacaaggtg catgaggacg ttgtagttgt
     7681 aacaaaagaa gaaatccgag cgttgttgga aaatgaaaac cctgccgata gcgtagagtt
     7741 taacgacaat cgtatttcgg catatatcgc acaacaaggc aactgctatg taatgaatcg
     7801 tcgaggaact ccgtccactc tgatatgtat tcacaaatca gacaaaggag ataaccttga
     7861 cagatattct aatctggcat ttgtggaaat tcccattgca aaggcaatcc taacagaatc
     7921 agcagacgaa gcaaaatcac tgttaaaagg ttatgttctt aatagtcagc agaagaaaaa
     7981 actgaacaga atacggacga attacggata ccagacaata actgggaaat aacttataaa
     8041 tctatttgga tatgatggaa cgccgtatga agcgaaagtt tcatgtacgg tgtgaagcgg
     8101 gggaaaactt ggagataaca tcaaaaagtt acctatcgct atagcgagga gggtggaaag
     8161 aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa cttgacgagt aagtcagaaa
     8221 atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa gaaagactcg gaaagtttag
     8281 caattgatat tctgactttt ttaacaggaa tttcaagtcg agacggggag aagtttcctg
     8341 tgttacgtaa agccattcga acggtgactc aaagtgatca acgggggtta ttacaagtta
     8401 ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa tatcgccgat catatcgagt
     8461 cctttaccga ttatgatttt gctcatttgc ttttctcaga cggctcgatt acccaatcga
     8521 ttagtttaga taaacagcta aatatcattc aagtggcgga tttggtttta ccggatgcag
     8581 aaacgacttt tgaagaatat accaccatgg aattgcttag tgtttcgatg ctaatcgtca
     8641 tttccacttt tgccttggac tttatccata gtaaccgaga aatcttcaaa attgtcgatt
     8701 tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa aacgttatcg aataagcttg
     8761 ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt cactcaaaat agcgacgatt
     8821 tattagatga acgtttaaaa aataatatcg ggttgaagtt tgcttttcgc agtaccgata
     8881 ttcatgaaat caagaaaacc ttggaattct ttggtttgga tcaggaggat caaagcaatc
     8941 aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAJSVD010000108         8858 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSVD010000108 DAJSVD010000000
VERSION     DAJSVD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.110"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(230..2611)
                     /locus_tag="ON498_002326"
     CDS             complement(230..2611)
                     /locus_tag="ON498_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3940556.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2601..3248)
                     /gene="recU"
                     /locus_tag="ON498_002327"
     CDS             complement(2601..3248)
                     /gene="recU"
                     /locus_tag="ON498_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR3940557.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3312..3851
                     /locus_tag="ON498_002328"
     CDS             3312..3851
                     /locus_tag="ON498_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR3940558.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3941..4360
                     /gene="gpsB"
                     /locus_tag="ON498_002329"
     CDS             3941..4360
                     /gene="gpsB"
                     /locus_tag="ON498_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR3940559.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4382..4749
                     /gene="rnpB"
                     /locus_tag="ON498_002330"
     ncRNA           4382..4749
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON498_002330"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4958..6124
                     /locus_tag="ON498_002331"
     CDS             4958..6124
                     /locus_tag="ON498_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR3940560.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6170..7666
                     /locus_tag="ON498_002332"
     CDS             6170..7666
                     /locus_tag="ON498_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR3940561.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7747..8430
                     /locus_tag="ON498_002333"
     CDS             7747..8430
                     /locus_tag="ON498_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3940562.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2793 a         1593 c         1872 g         2600 t
ORIGIN      
        1 gtgtatttag ccgatttaat tataaacaag taaatggata aagtatatgt aatgataaaa
       61 gaaaaatgga ttttctaatt tttaagctaa atgttagatc acacatgtat atgctggata
      121 gtcttataaa aaatcatgta atacagcttg gtacatgatt tttatttgta gctgtttttt
      181 acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac tatctgtttc
      241 cagaagctga ggtgctgctg gatggtgtat ggtcatttgc agcatccgct ccgctagttg
      301 ctggctcact ggaagaagca ggaggagcag atgaagacgg tgtcgagctt tcttcggatg
      361 aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt
      421 ttgaggactc agcagttgat tcgctttgac tagtagagct agagctttga gtcgttgtct
      481 ctgttgttga gcttccagga gtctggacat agctggaaga tggaatagtt gttgatggtg
      541 taattgcatt cgatctagct gtatattgat ctttgtagta cagttcaccg cccacacgaa
      601 ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca tactgcataa
      661 gctcgcgata aacatcagag gctacgtgtg acgattctga agtgacaggt gtcatttttt
      721 tattgtaacc tgtccacacg gaaatcgaat aatttggcgt atagcccgca aataggatat
      781 ctggatacac cccagaagaa ataccaagtt ttgcatactc gtcatctgtg taattggatg
      841 ttccggtctt accggcttga tataacccag cgatttgcgc attcgtacca gttccttcag
      901 tgatcgtatc ttttaaaatg tctgtgatca tataagctgt ttctggagac atggctgttt
      961 ttccatctgg ctcataagtt tcttctgttc cgtcttggaa gacgatttta ttaacatatt
     1021 gaggtttgta ataagtccct ccattggcga aagccgcata ggctgctgcc atttttaagg
     1081 aagaagctcc gtattttgtc ccatcttgtt cttccgtatt actagagatc gcattcgatt
     1141 gatggatcgt actgtattcg atcccaagat tttttaagaa agaagccact ttatctgaac
     1201 cgacttcatt gaataatttg actgctggaa cattacggga taagtaaagt gcttggcgta
     1261 aagtaatggt gcccatatat tgattatccc agtttcctac aggagtcgaa gtcccttcat
     1321 agttatacgg tgcgtcagta atagtttttc cagtagaata tttcaaatat tcaaaagctg
     1381 gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta ttcactgcta
     1441 agttgttgcc taaagttacg tcttctgcga tgtgacgtcc gccaatctgg gcttttactt
     1501 tcccggtatt ggtgtcgata agggtggagg caacttgcat ctcgtcgtct gggtaagaga
     1561 catactgatc cgtgttgacg atatcatata gtttcttttg ggcgtccaga tccaaattcg
     1621 tataaatctc taaaccgtct gtgtagacat tcttatctgt tttttcttgt acttcattga
     1681 tgacttcttt gacataatta tcgacgatct tagtattgtc atcggattga gttagttctt
     1741 gcagaccatc cgttaccggt acattaacag cttgatcata ttctgtttga gagatttttt
     1801 cgttttgaag catcgtatac aaaacagtat ctcgtctttt cttcgcctga tctggataaa
     1861 cataaggatc ataggcggaa ggggcttgag gcatcccagc aagcaatgct gtttgcggta
     1921 aagaaagttc tgataatttc ttaccaaagt acatctcaga agctgtctcc atcccgtaca
     1981 agccgtttga catatacact ttatttacgt agtaggtcaa gatctcttgt ttcgattttt
     2041 tttgttccaa tcttacagcc atccacgctt cttgggcctt gcgtttcaga gtctgatcct
     2101 cggcactggt ggagaagaag gaaagtttga tcaattgctg tgtcaaagta cttccgcctt
     2161 gcagtccgcc agaggtaaag ttagagaggg cagaaccgat gatccgaata gggtcaaccc
     2221 cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat cgcatcttct aatgttttcg
     2281 gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg aatagttcgc
     2341 catcttgtgt atatagtttg gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg
     2401 gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg atacagaaaa
     2461 atgagagtat tcctaaaagg atcttgataa ataatccttt tttatgtgtt ccagaggact
     2521 ttccagaacc tttgccagaa ttcttcttca cactcttttt aggagtaggc tgtttctgtc
     2581 gtctagagct tctagtttgt tcatttgcca ttcttgcttt ctccttcaat agataatgtt
     2641 gataaataga agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc agttttgatc
     2701 tctattccta gttcttcgat caaagataaa ggtagtgatt tgcgtccttt ttccttttgg
     2761 gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa aacagcgctt caaagacgaa
     2821 aaccataaca agacaaagca gaccccattt tgttcgaggc atgctttcat atgttcgatt
     2881 tgatgggcat ggaagttttt aaagggaaaa gatgttttgt tcttggtttc ttttgcttca
     2941 aagtccaagt aaaagcctcg atagacgcca ttatagtctg tagttgaggc ttgggtaaag
     3001 taggcttcct tgatcacagc agcacttctc ttaggatagt ctactttcac gatttggata
     3061 ggggtaggct ttttatggat aaccgccaac ccgcgggaaa gataatattt attactttca
     3121 ttgattgctt cttcaaagcg catcccccga ttgccgaatt cagtgatctg atttcttttt
     3181 tcttgaattt tttcgacggg agaggcattc ttataataag gttggccatt tggatagcgc
     3241 aataccatct ttaatcacac tcctaggcca cattatagca aaaaagcgga tagaaagaaa
     3301 aggaatctga gatggataag tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac
     3361 tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag
     3421 aactccatca gttagcagaa gaagggctgg aatgggtact tgtcagtggg aatttgggcg
     3481 tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga agaatatccg gaactatctc
     3541 ttggtatttt gtatcctttt tctgattttg gtaataactg gaatgaaggg aaccaagaaa
     3601 aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc tgttagccat caaccctacc
     3661 aatcacctag tcagctaaag aatcacactc gttttatact tgagcataca gtgggctgtc
     3721 tgctgatcta tgatgaagaa ttccctggaa aaacgaagta tttttttgaa gatgcacaga
     3781 attaccaagc agatcatccc tacgaaattc ggctgatcag tatggacgat cttcaaaatt
     3841 ttagtgaata atcgatattg tgtttgattt atcaactttt ttcctataca atgaaaaagg
     3901 tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat atggcgaatt tggtttatag
     3961 tcctaaagac attttacaac aagaattcaa aacaaaaatg cgcggctatg atcccgttga
     4021 agttgatgaa ttcttagaca atatcatcaa agattatgaa acttacagca aagaactcct
     4081 tgctttacaa gaagaaaacg atcgattgag tgcaaaagta gctcaattat ctaaaactca
     4141 aggagcagct caaacacgcg tgcaacaaac agaggcacca aagagcgcag cagtgaccaa
     4201 ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga
     4261 tcaacaagca agtgcagtca aaccagcaca acctaatcca aataactaca caaatgcaga
     4321 cacaagctta gatgataatg aaaaaacacg tcaattttaa tttttgatta atgaatttgc
     4381 ggttttcggg taatcgcggt ctgacttgat caggctgagg aaagtccatg ctcgcacagg
     4441 ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag ctaaggtact ctttggagta
     4501 acggcaggaa aacaagctaa ggcttcggct atgcttaagt atccttgaaa gtgccacagt
     4561 gacgaagcgg ctggggaaac ccatccggtg gaacgcggta aacccctcga gcgagcaacc
     4621 caaacaatgg taggggcgct ttttgtctgg aagaagaaca ggacaaaaag gcagtataac
     4681 tgcagataga tggttaccgc tttgtttttt ctccctggaa aagcaaagta cagaacatgg
     4741 cttatagaaa accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg cgttataaga
     4801 aacaagaaaa acatttgaaa gctatttttc aatacttcag atgaatcgtt cttattacaa
     4861 aacaaacttt tgtatcaccg gttattcgga atttgagtaa aagtgtgggg taggactgat
     4921 gtcccacctc ctttttttat aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa
     4981 cctcgtcgca actgcggcaa gcgggctaga agcactggtt ggtaaagaat taagagatct
     5041 tggaatcgaa tgccaagttg agaacggacg tgcacgtttt gaaggaacaa tggaaacgat
     5101 cgctacagct aatttatggt tacgtacagc agatcgagta aaaatcgtgg ttggggaatt
     5161 cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa gcattgcctt gggaagattt
     5221 tcttccacta gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga aactatatag
     5281 cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta
     5341 tcaccgtcca gcttctgtac cactgacgga aactggcgca cattttcagt tggaagtagc
     5401 tttgctgaaa gaccatgtga tggtaactct ggatacgaca ggaccaagtt tattcaaacg
     5461 tggttatcgc ttagaaaaag gtggagcacc attgaaagaa aacatggcag cagcacttat
     5521 tatgttgaca aattggcgaa aagaccgtcc attttatgat cctgtatgtg gttcaggtac
     5581 gctttgtatt gaagcagcct taataggcca taatatcgca ccaggattca atcgtgagtt
     5641 tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg gaaaatgttc gatcagcagc
     5701 cgaagaaaaa gcagattatg atattgaatt agatattact ggttcagata tcaatggacg
     5761 gatgattgaa attgccaaag ccaatgcaga agaaatcgga ttaggctctt cgattacctt
     5821 taagcaacaa gcagtcaaag acttcaaaac ggataaagaa tacggggtca tcgtggctaa
     5881 tccgccttac ggagaacgtc taggagaaga agaaactgtt cggaaattat ataaagaaat
     5941 gggcgatgta ttccgtccat taaaaacatg gagtaaatat atcttgacta gcgacttagc
     6001 gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt
     6061 gcgtacggat ctattccagt attggggaac tcgtccgcct agaaaaaaag agaaagacga
     6121 ataatcttac taattgattc attcaattgg atgtttggag gaatagggaa tgaaagaaaa
     6181 agagttttta caagaagtaa aagaaatcaa tcttctacaa caagctttag gaatcctcga
     6241 ttgggataca caaacaggta tgcctgaaaa agctagtgca taccgaagtg aagtagacag
     6301 ctatctttac agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg
     6361 ctatttttct gaacatccgg aagaattgtc ggaagttgga actgccgttt ttgccaaagt
     6421 aaaagaagag tatgatttgg aacatcgcgt accggaagat cttatgagaa aatatacagc
     6481 tgccgtttct tcagcacacg gacaatggca aaaagctaga gaaacacaac aattttctga
     6541 ttttcaacac gcattgaatc aaaatattga attaacaaaa cagttgatac cttactggag
     6601 aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa tacgaacccg ggatgactgt
     6661 cgaaatcctt gataaggtat ttgatcagct taaagaagga attctttcga tccgccgtac
     6721 gctccaagaa aaagggactg aacctgaaac agacttttta aatcggagaa tgacgaaaaa
     6781 acaacagaag agatttgtag tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg
     6841 tcgtttagat gacactgtcc acccatttat gactggaatt aatccgaatg atgcacgaat
     6901 cacgacacga tggaacgaaa cagatttcaa aatggctgtt tttggagtga tccacgaggc
     6961 tggtcatggt atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga
     7021 aggtacatca atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag
     7081 tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt
     7141 cgatgatatt ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt
     7201 tcgtatcgag gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga
     7261 aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa
     7321 atatgaagag tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt
     7381 tcattggtca ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc
     7441 ggcacaatta cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga
     7501 cgattattct ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag
     7561 aaaaccaaat caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat
     7621 cgactatatg aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa
     7681 agatagctga aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg
     7741 gaacgaatgg ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat
     7801 caaacaggac atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg
     7861 gagacggaac cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg
     7921 attgatgata aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt
     7981 cgctctttgc caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg
     8041 tccttttcta aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa
     8101 gacgctgatc gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc
     8161 ggtggacatg ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa
     8221 gaaaattacc gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg
     8281 tctgataaaa tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg
     8341 cgtgaattct tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca
     8401 gacaattgga tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca
     8461 agatatttcg gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg
     8521 tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt
     8581 tgatgaatat tgtaacttgt gattcggaat tggcaggaga acaccgttta ttcggaagaa
     8641 taggactgtg acaagacttt tgtcacagtc cttttttcga attcaacaag taaaactttt
     8701 aagaaatatt cagagagtaa aattaaaaat tgctgataaa cgaagatgga aattttatgt
     8761 atttttattg gtctatgcaa taaataaatt aaatattcag aatacaattg attttaacga
     8821 aagggcttct atcaatggtt gtgttgtatt tatctgcc
//
LOCUS       DAJSVD010000109         8835 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSVD010000109 DAJSVD010000000
VERSION     DAJSVD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.111"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(133..306)
                     /locus_tag="ON498_002334"
     CDS             complement(133..306)
                     /locus_tag="ON498_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3940563.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="ON498_002335"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ON498_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ON498_002336"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ON498_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3940564.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ON498_002337"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ON498_002337"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR3940565.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ON498_002338"
     CDS             5704..6489
                     /locus_tag="ON498_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3940566.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ON498_002339"
     CDS             6502..7353
                     /locus_tag="ON498_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR3940567.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ON498_002340"
     CDS             complement(7477..7929)
                     /locus_tag="ON498_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940568.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ON498_002341"
     CDS             complement(8394..8762)
                     /locus_tag="ON498_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR3940569.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         2966 a         1487 c         1835 g         2547 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaa
//
LOCUS       DAJSVD010000110         8809 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSVD010000110 DAJSVD010000000
VERSION     DAJSVD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.112"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            34..333
                     /locus_tag="ON498_002342"
     CDS             34..333
                     /locus_tag="ON498_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940570.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(430..864)
                     /locus_tag="ON498_002343"
     CDS             complement(430..864)
                     /locus_tag="ON498_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940571.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1096..1806)
                     /locus_tag="ON498_002344"
     CDS             complement(1096..1806)
                     /locus_tag="ON498_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3940572.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1796..2656)
                     /locus_tag="ON498_002345"
     CDS             complement(1796..2656)
                     /locus_tag="ON498_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR3940573.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2661..3299)
                     /locus_tag="ON498_002346"
     CDS             complement(2661..3299)
                     /locus_tag="ON498_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3940574.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3314..3613)
                     /locus_tag="ON498_002347"
     CDS             complement(3314..3613)
                     /locus_tag="ON498_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940575.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3656..5140)
                     /locus_tag="ON498_002348"
     CDS             complement(3656..5140)
                     /locus_tag="ON498_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3940576.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5160..5621)
                     /locus_tag="ON498_002349"
     CDS             complement(5160..5621)
                     /locus_tag="ON498_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3940577.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5639..6706)
                     /gene="ulaG"
                     /locus_tag="ON498_002350"
     CDS             complement(5639..6706)
                     /gene="ulaG"
                     /locus_tag="ON498_002350"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3940578.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6924..7694
                     /locus_tag="ON498_002351"
     CDS             6924..7694
                     /locus_tag="ON498_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3940579.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7967..8794)
                     /locus_tag="ON498_002352"
     CDS             complement(7967..8794)
                     /locus_tag="ON498_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940580.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2668 a         1854 c         1388 g         2899 t
ORIGIN      
        1 ttataatgta tcagtataga aaggaaataa aacatggatt atactgttga atttgacagt
       61 gaactactta agcaatttcc acaagaagaa catttcatgt ataaagaagc aatttcccac
      121 aaagtgacag agtatttgga gcataatttt tttcgtataa agccagtgcg tactcgttcg
      181 gcaaaaggaa tatacgagat gaaagtcaaa attaataaaa aaaattatcg catagcattt
      241 gctttaaaaa aaaatcatgt gttcattttt tatattagtt cgaatttgca aaaaattgta
      301 tttgataaag aggtgagtaa aaaaataaaa tagctgagca ttggaggata gattgagtga
      361 tactaagtct atcctcttgt ttttctaaag aactaagcta gaattttttc ttttgtacgt
      421 tattatatgt tatttgtcat taccaaaaag cacctttttt cttctagtat ctatgacata
      481 agaaatacca cccatttcct ttacttgaac aaatcctact gattcataaa atttttttgt
      541 tttctcagta tcatctgtca ataattcgat ttggactggt ccataactag tgatcttctc
      601 caaagcttca ttcaaaagca ctttgcctat tccttggcgc tgatacctcg gatgaatgag
      661 gatatcttga ataaatgcga tcgtcgcaaa atcagtgatc caacgaacga ttccgataat
      721 cttcccttcc ccattacgct ttatcagaga ttcagattgt tcaaatgctc tagctaacct
      781 tgcagtgtct tttgtgtaat tcgtccaacc aacgctctta tataattcag aaatctcatc
      841 gatcataagg gaatcaatat tcattttttt cttcctttct tatacaattc tccctctaat
      901 aatacagttc tttgactatt taatctatca catataactc tcctgtaaag attctctcga
      961 ttccttaaaa ttcctctttc cttttagaat gcatactttt tgataaataa aaaggttgtg
     1021 ataaattttc ttcacaacct ctgtttctct ctatcaagtg ttatctttta attttctacc
     1081 cttaccctag catctctatt gcccgtagta tgcatacttt ccatgtttgc gaaaataatg
     1141 tttatctagt aaatattgcg ggatttcaca tatattgggg ttgatcagct cgttttcttt
     1201 ggccatcaga caaatttcat ctaagatcaa actgttttct actgctttca taggtgaatc
     1261 cccccatgtg aacggtccat gtccatagac aagtactcca gggacttcgt ttggatctaa
     1321 ttttctttcc ttaaacgtct cgacaatcac attccctgta tgaacttcgt aagcttctct
     1381 tacttcttct ttcgttaatt ggcgtgtaca aggaacctta ccataaaaag catcagcatg
     1441 agtcgtgcca taagctggca aatcgcgtcc tgcttgcgcc cacatgacgg agtgcgtgga
     1501 atgagtatga gtaatagcag ttatatcttc aaaagtttga tacaaaacaa catgtgtggg
     1561 gagatctgaa gatggtttta agctatcttc ttctattata tttcccgaca aatctgttac
     1621 caccatttga tctgcttgca tgcattcata tctgacaccg cttggcttaa tgacgatgac
     1681 tcctagctca cggtccactt cactcacatt gccccaagtc aatttgacta atccgtatcg
     1741 cggcaatgcc agatttgctt caaggacacg ttgcttcatt gctgcaattt tttcttcaca
     1801 agacatcata gccagcctcc ttcaatttag aacttaagta gtcttttgcc tgctggattt
     1861 ctcttcgaaa gtcttggctt ttttcactcc acatttcaat cagaaatgtt ccatcataat
     1921 ctagacgttt caaatgtctt aataatccaa gaaaatcaac acaaccgcta ccaaaaggaa
     1981 catcacgaaa ctggcctgaa tattcttttg ttacagggta agtatctttt aagtggatag
     2041 cagtgatata atcgatccct ttctcaagtt ctcgagccgg attattttcc ggccatgcgg
     2101 acaaattgcc taggtctgga tatacttgta gaaaaggtga atgaatctgt tctttgattt
     2161 ctaaaaattt tgaaatggag ttcataaatg gatcatccat gatttccacg gaaagaatga
     2221 tgccatattt agaagcttcc tttacgcttt cttttattcc ttcgataaag tcttctctgg
     2281 aattcattga tttctcttca taataaacat cgtatccagc tatttggatt actttgacgc
     2341 ttagattatg cgccaaatgg attgcttttt tcattagttg tttcgctgct tttttctttt
     2401 ctggatcagc agaaccaaac ggaaatcgcc gatgcgcact taaacaaatc gaatggatac
     2461 gtacaccgga gctgaacatc tcttctctca gctcagctat cttttcttca gaccaatcta
     2521 atcgtgctaa ccgctcatcc gtctcatcaa tcgacatctc gataaaatcg aagtccattt
     2581 cttttacaag ggaaaaacgt tctttccatg aaatatcttt gggtaacgct ttttcataaa
     2641 tcccaatttg agccatttcc ttacccccag atacgaatga tctcatcttt gaagttttgt
     2701 gcggccttca tcggatcatt tgctgctgta ataccacgac cagtgataaa ggtatacaca
     2761 tcgatccctt caaataagtg taatgtgtgt ggatttaatc caccagttac agaaacatta
     2821 aagcctagtt caatcagttt tttgatcttc gataaatctt tttcacccca agtttcacca
     2881 gataacagcg catcacggct ttgatggtag attacctgcc ggataccgat gttatgccaa
     2941 tccatggctt gttcaaaagt ccaattgccg tagagttcta cttgcaattc acctatttct
     3001 ttttgtgctg cctccatagt tggaatcgtt gcacaacaaa tgactgtcat ccagtctgct
     3061 cctgcttttg caacattact cgccactgtt cctccagcgt ctgcacattt cgtatcagcg
     3121 actagttttt tatctgggaa catactgcga atacagcgaa tcgctttttg tccttcctgt
     3181 aagcaaagaa ttgtgcccac ttcaataata tctactacat caccaacttt cataacatct
     3241 cctaatgcat gttctaagtc attgtgatct aacgcaattt gcaaattcgg cctgctcatc
     3301 ttagttcctt cctttacttg tttttttggt accactttgt ttcagcaaat ttttcactga
     3361 tttctttgac tgacatcaca tttttgatac cgataactgt aactcctttt ttctctgcct
     3421 gagtaaacat attcaaaaaa ttgactggcg taaaaactac atcgtattgg ccagctgaac
     3481 tttttccctc tgaaatggca caatgatgga ttttggtaat tggtacgttt agttctttca
     3541 ttgctttttc cacacttctc atcatcatca agcttgttcc tgatccattt gcacacgata
     3601 ctaaaattct catcttaatc cctccattat gagtgttgtc taatcagatt ttcttttata
     3661 ctctctgctt ttctgcatat gtgtcatagt cttctacaat caaaaaataa ccatcaggat
     3721 ttttccgata ttgtaactgc ggaatagcca ataaaccaat gacaactacg gcaattccag
     3781 cataacctag gactttcatg attactgtaa acaatggcca agctgttgcc cagtcgaaca
     3841 tcccgatata accaccgtat gttgacatcc caatccatgt agcgaataat gctgaaccgc
     3901 ctacttgtat gattcctgaa agaaacggga acaggcaagc ggctttgaat ccacctcggt
     3961 tattggcata aactgctata acggcattat caaagaacaa cggaatgaaa ccggctatga
     4021 cgatcgttgg tgatttcaat aaaatcagta aaccaatcat caaaaactgt cccaaagcac
     4081 caaacaagaa cccaatcgtt accgcatttg gcgaaccaaa gccaaaagta gcagctacgt
     4141 caattcctgg aacagctcct ggtaaaatcg tgtttgagat tccttggaaa gattgagtta
     4201 gttcatcgac gaatgtccga acacctaatt gcaagatcgc cagatatacc gcaaaattta
     4261 aagaagtcgt cataatgtag aataagaaac tctgcccttc ttgcatgaat tgcgcttgaa
     4321 tcaaataatc tttgcctagt acaatcagaa tgatcccaaa gaaaaatagc atgagtaacg
     4381 aagtagcaac catattctca ttgaaaatcg acaagaatcc tggtaatttg atatcttcaa
     4441 tttttttgtc ttgcttgatt gatctatttt tcttttcatc ccgctttttc atccactcag
     4501 ctaattttgc aaatataaag attccgaaca tttgctgatg tgccactgca aatccggcac
     4561 cttctgtcaa ttcctgcgtg attcctactg ttaagttaga agctactgtc caataacaac
     4621 ctaaaatcag tcccataaaa atcaatgctt gtatcctacc tagatcagga aagcaaaaca
     4681 gtaaaatcca aaaagcggta gctgcttgtt gaacctgaac gtttccggta gtgaaaacgg
     4741 aacgcagttt cgtatatttc tgaaaacgga ctaataagat atttaaaaca aatgcaatca
     4801 gtaataaaat catcacatct ccaaaggtac ggccaaatgt ctccattaat ccagcatcca
     4861 cagcattttg tccaaaataa ggatctgtaa ccatggcatc caaattgaac cgttccttta
     4921 gtccaaccaa gattggccga aaattattga ccaaaccacc agagccaaca gtcaaaataa
     4981 agtaaccgac cgttgccttt aagaatccag cgatgcattc atgaagcggg cgtttcagca
     5041 aaatatagcc taataacacg ataaaaccaa tcaggtatgc tggctgcgtc aagatatttg
     5101 ttgcaaaata cgtccaaatg cttaacaaaa attcccccat cttttttctc ctctatcaat
     5161 taattatatt ctgccatcac aacttggtaa tcatcgatgg agttcgtaga gatgagacgc
     5221 tcaaccaatc catctgtcat taataattcg gataattttt ggatattttc tagatgttct
     5281 tctggatctt ttgctgcgag tgtaaaaaac aaactagccg ttttctcctc attctcccct
     5341 tcactttcaa aacggattgg ctgtttcaac ttggtaaatg aaatagctgt tccaaaaaca
     5401 ccatcacttt tttctgaaga atgcggcatt gctacttgag gtgcgatcac aatataagga
     5461 ccgtattttt ctacacaatg gatgatctcc tccacatacg tttcatcaat aatttcctta
     5521 tcgatcaata caagacagct ttcggcgata gcttcacgcc aatccgtgat ttctttatct
     5581 gggtaatgaa tcaattgatg gtcaaaaaag tatttcagca agaaactccc cttctttctt
     5641 ataaaaacga cggaaacggt gtatcatttt caatcgagaa gacatcttca aatcctcgat
     5701 aaaaattaaa ctcaagatca tctttgtttg tcggatagat gaatttcccg cctacttgcc
     5761 agatataagg tttaaatcca tacttcaggc gatccttttt gaatttccaa atttcagtga
     5821 tctcttttgg atcagccata aaattagacc aaatatcgta gtggacagga atcactactt
     5881 ttgcattcaa tgactccgcc atgcgcagca tatcaacaga agtaactttg tctgttatcc
     5941 ctcgtgggtt ttcaccatag gcccctaaac agacatcgat tttgtgttca ttaccatgtt
     6001 tggcaaataa attggagtaa tgtgaatctc ctgcatgata aagattccct cctgatgttt
     6061 cgaataaata gtttactgca atttgatcca tctcctgagg catttttcct tttaatacct
     6121 cttctggatc ttcacaagtg accaacgctg tccgatcaaa cgcttctaaa gctactacat
     6181 tgatgtcttt gattttgact gcatcacctg gctttactgt aatcgttttt tcttcaggaa
     6241 ttccccattt ttgccacgtg cgaaccactt cttttggccc aatgaatgtt gcttcaggac
     6301 aattttgatg aactgctgcg gctgtatgga tatctagatg atcggaatgg atatgtgtaa
     6361 cgattaaagc gtctacattt tttatcgcaa aaggatcgat tacgaaaggc tgagtacgta
     6421 aattaggctg catattttga acgccactca tacgcatcat ctgatggcct tttttcatca
     6481 tgccattccc atgactacgt tttcccgtcc cacaccatag atcacataaa atattcgttc
     6541 cttcatgact tttcagccaa attcccgtac agcccagcca ccacatagca aaagttccag
     6601 gatccactac ttctgtttcg atttcttcat tcaagtatgt gccccactca ggaaatgttt
     6661 ctactaacca cttttctttt gttacttcat tgacagtttt actcatttgc ttgctccccc
     6721 catatcgttg ttccaactta caagtaaata ttaatgtgta tcagcatcca tgaaaaccct
     6781 tttatatgac cgtttgtttt ttatatgatt ttctttcagt aatcaatgat tgtttcagta
     6841 taataaagac agatatgaat gtttaaatga acaactagtc aaataaatcc atggatcaag
     6901 atgtccctca ggaggcaaaa gtcatgaaaa actcacttgt caatattgaa aaacgtcaaa
     6961 aagaaatttt ggaactgctt cagcagactg aacaactatc aactttcgaa ttagctgaat
     7021 cccttgatgt atctattagt acgatacgaa gagatttaaa tttattagaa gaaaaaaacg
     7081 agatcatacg caattacggt tattgttctt ataattacaa aaaccagact gattttgatc
     7141 aatctggtcc tgaaaggatt aaacaagcga ttgccagaga agctagtaaa tacattaacg
     7201 attatgatac gctgtttatc aattctagct cgactgcgtt gaaaacactg gattttcttc
     7261 aatctaacca tttgacagtc attacaaaca acgtgaaaat caattactct ccgcacaaag
     7321 ataattgtag ttatatttta actggtggag agatgcgctt tccaaaagaa gcactagtgg
     7381 gcgacatcgc attaaatacg atttcttcag tcaatgcaga catttgtatc attggctgta
     7441 gcggagtcga tgtagtacat ggggtcacaa caaagatatt aaatgaatca acgatcaacg
     7501 aaatgatggt caacaagaca atcaaatgta agattttagt cgctgatcat cgaaaaatcg
     7561 gactgacatc aaagttcaag attgctgata tttcagtctt tgattatttg atcactgata
     7621 aattttgttc tgaaaaagta ttagaggaga tcagaaatac ggggataaaa accattcaag
     7681 tagattcatt ttaagaacca ggacgtaaat atgtaaatag gattatcaaa aaaatgaata
     7741 acaaatttat cgataaatga aaaacacgct acgctcaaat tgataaagga atcccaagat
     7801 gtatccatcc aatcgatacc atcataatct attgcttctt ttataaccgt tatatgttgc
     7861 ctaaatagga atcaaacaat atcgaaaaaa ggaggtatga caaaagtcac acctcctttt
     7921 ttcgaataaa cggtattcaa atcagaacac tgatttcaca accactttat ttcaggtcgt
     7981 tttcaatcac ctttaccgcg taatcatttc cgttgaaacg cgctattttt tttgcgtcca
     8041 tatatctttc taaataatca tcttctccta attgcttatg gcaagaagca atttcataaa
     8101 gcaagtattc caatgcataa gtcgatttat gtttcttatt atactcaata cccttctcac
     8161 ataattggat tgctttatca taatcctcga ttttacggta ataggtggcg atatcataat
     8221 atacaatgca gaccatcaga ttatcaaaat cagtctcact caatatttta agtgtacgct
     8281 ccacataaac tttcgcaaaa tctaattctc cttttgacaa gtagatcatt gccacgtttg
     8341 cagctgcttg taagtgatgg atatctgttt cggtcgtttg ttccaacact tgattaaaat
     8401 aaaataatgc tgcatctggt tttttcgcat aaagatgatc aagcagtcct tgataaaata
     8461 aaatttgatt taaaagatca gaattactta ctttttcctg atcgatcgtt tgtaaaattt
     8521 tataagattc ttggtatttt gatacagaat tcaatgattg aatctcattt atttttttcc
     8581 tgattttgtc ttcttctgtt tctactaata tatcggagat ctcgacattc agctttttgc
     8641 aaagcgcact taagatattg atgcctggcg acatattctt gttttcgata ttgctgacag
     8701 ttgcttgttc acaaatgcct tctgctaatt gtacttgtgt taaccccttt ttctttcggt
     8761 attcttttag taatgctcca tctactttaa tcaaaacaat ttccttccc
//
LOCUS       DAJSVD010000111         8745 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSVD010000111 DAJSVD010000000
VERSION     DAJSVD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.113"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..125)
                     /locus_tag="ON498_002353"
     CDS             complement(<1..125)
                     /locus_tag="ON498_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR3940581.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="ON498_002354"
     CDS             complement(143..463)
                     /locus_tag="ON498_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940582.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..1538
                     /locus_tag="ON498_002355"
     CDS             762..1538
                     /locus_tag="ON498_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3940583.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1553..1756
                     /locus_tag="ON498_002356"
     CDS             1553..1756
                     /locus_tag="ON498_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940584.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1772..2110
                     /locus_tag="ON498_002357"
     CDS             1772..2110
                     /locus_tag="ON498_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCR3940585.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2097..2276
                     /locus_tag="ON498_002358"
     CDS             2097..2276
                     /locus_tag="ON498_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940586.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2319..2789)
                     /locus_tag="ON498_002359"
     CDS             complement(2319..2789)
                     /locus_tag="ON498_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940587.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2876..3574
                     /locus_tag="ON498_002360"
     CDS             2876..3574
                     /locus_tag="ON498_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3940588.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3752..4093
                     /locus_tag="ON498_002361"
     CDS             3752..4093
                     /locus_tag="ON498_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940589.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4086..4757
                     /locus_tag="ON498_002362"
     CDS             4086..4757
                     /locus_tag="ON498_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3940590.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4763..5449
                     /locus_tag="ON498_002363"
     CDS             4763..5449
                     /locus_tag="ON498_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940591.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5452..6201
                     /locus_tag="ON498_002364"
     CDS             5452..6201
                     /locus_tag="ON498_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940592.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            6213..6482
                     /locus_tag="ON498_002365"
     CDS             6213..6482
                     /locus_tag="ON498_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940593.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            6488..6652
                     /locus_tag="ON498_002366"
     CDS             6488..6652
                     /locus_tag="ON498_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940594.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            6645..6944
                     /locus_tag="ON498_002367"
     CDS             6645..6944
                     /locus_tag="ON498_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940595.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            6944..7246
                     /locus_tag="ON498_002368"
     CDS             6944..7246
                     /locus_tag="ON498_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR3940596.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            7286..7651
                     /locus_tag="ON498_002369"
     CDS             7286..7651
                     /locus_tag="ON498_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940597.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            8141..8281
                     /locus_tag="ON498_002370"
     CDS             8141..8281
                     /locus_tag="ON498_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940598.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            8278..8466
                     /locus_tag="ON498_002371"
     CDS             8278..8466
                     /locus_tag="ON498_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940599.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            8474..8668
                     /locus_tag="ON498_002372"
     CDS             8474..8668
                     /locus_tag="ON498_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940600.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         3220 a         1366 c         1755 g         2404 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 gatattaccc tcaaatggat ttagaggaaa ttggttagaa aggagtttta gtatgactga
     1561 cattgcagaa atcactcaac gagatagaga aaaaatcaaa gaatatgtcg aaagttcaaa
     1621 gttcttaact tacaccatgc ttgctgaaag atttggaatt agcaaaagct acttatcttt
     1681 aattttaaac ggtaaaaaga cttctgcaga agcaaacaga attatagatt cgattatcac
     1741 tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat ctagaagcaa aaatcccaat
     1801 tccagaaggc tatgtaatta tctcccaagt ggattatgag gagttaaaaa aagctgatga
     1861 tactggtaga tggatgacgt tgccagaagt gctagaacgg attaacagaa aatatgattg
     1921 gtttacttct agagttttaa agaacccaag atatagaaat attatcgata tcgagaaaaa
     1981 caaaaatgga tttgtctatt atccagttga aggaagagac acatatctat ttttaagaag
     2041 taaaacactt gaatttttag aaacaaattt ttcggaaatc ttaaggaggc aagcggatgg
     2101 gaaaatttaa tagagcgcta gtgttcagcg caccgctaat catctacgct ttaggacttt
     2161 ggggaagcag gcaagcgttg ataggaacga ttgtttacat ggtttggatt tttatggggc
     2221 ttgatgaagc tgaagctgag tacagagcga aaaagccaac cgagggggct gactaaaaat
     2281 gaatagaaaa gagaaactag aatgtatatt actattactc agtttaattc tttcactaat
     2341 ttctctattg ggaagtttgt atttttgata tcaacaattt tgtggatttg ggttctattt
     2401 agagtaaata tcatttttgt ttggtgatta aggagtttat taaaattaat tttccctact
     2461 tcaaatgcaa ggtaacaaga tatagcactt ttaggaggaa tatttagcgg tacagcttca
     2521 gagtatattc tattatcatc gactgatcct cctttagcaa ttaatttctt aaatggagta
     2581 gctgaccatc tgcggtttaa gttagcggat gaaagttcta aattaactaa catagctggt
     2641 tctgttgaat agttagaaat gattactttt gtataaatca tatctgcagc gaagtaagaa
     2701 gcattaagtt caacttctag ttgagggcgc attcctttta tttttatccc tgttaaaaac
     2761 gtactcaata taaaacttat tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc
     2821 aaaataaaca ccaccagttt ttaactaaat tataccaaaa aggagagaag aaataatgca
     2881 agaattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag tggcagaaag
     2941 cttcgagaaa aaacatcaac atgttttaag agatattgat gcactaaaaa aagatgtgtc
     3001 caattttgga cagatgtttg tagaaggtaa tgaaccagat tcatatggca gaaatcgacg
     3061 agttttcttc attagtagag atggattttt cttgctggct atggggttta caggaaagaa
     3121 agctatctac ttcaaacaaa aatacattga agcattcaac gaaatggaag atgttattcg
     3181 caagaatact gttcctcaaa caattgaaga tatgatgatc tatcaattag aagaaatgaa
     3241 agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg gacaacaaga
     3301 gtttgaaatt aagcaaaaag gaaatatgaa agttatggga gttctagggg gaaaagaaag
     3361 ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg
     3421 taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc
     3481 atttattgaa atgtggttgc cagaaactgc gatccgtatg gaaattgatc aactgaacag
     3541 acaacagaga ctttttggtg atgacaatga atagagctga agcgctaaga atagggacgg
     3601 caattgctaa ttgctggtgg aaatactata aaccaatcat cctaagccaa caacatattg
     3661 acaagcaaaa agcatggcaa caaataaaaa agtgactccg ccggcaagca aagagtcaca
     3721 aagaaaacac atcataagga gattttagca tatggaaaaa gaactttcca ctctagatca
     3781 atatttgatt gatcctgatt ggggcaagcc gaaaattgag gaaacaagtg gtcgaaaaat
     3841 cagacgaaat cttttgacga atgaagaact agcttgggat caagatgatt taggcaacca
     3901 tgtaactatt tgggatcatg tttatcttat ccatctatcg aagcattcga ataaacctga
     3961 atatatttac gtcatcgaag atggcttgat tgatgcgcta gaagagtacg acagagataa
     4021 cttgattgat atctcttatt acggaccagg taagaaatac attgctgaaa tggaggcaga
     4081 atttgatgag tgaaatcaaa gggacaacga actttgaaaa actttttagt cgtaagttaa
     4141 ataaaattct caagaaaaaa ggaaattttg attatttatc ttgggctcac gcgtgggaga
     4201 ttatgaaaaa gaatgatcca caggcaacgg taactattaa tgagtacaaa cactacaggg
     4261 ttgtttctgg aactcatcaa gactttcttg ttgaggaata taaacctttt cttatggatg
     4321 aaactgggac ttatgtatct gtctcagtaa cggttaaagg acacacggaa accgaattat
     4381 ttcctgtttt agattatcga aaccaaccag ttgttaaacc aaatgcaatg caaatcaata
     4441 actcattgaa gcgatgcttt gtgaaagcat tggctctaca cggactggga ttatatgtat
     4501 ttcaagggga agatattcca acaccaccta gaatcgatac aaagaaatta aacatgctag
     4561 agacgattct agaagctttc aatgagcaga tgggtaaaga tatgaccaaa accttaatcg
     4621 aatatgttaa tgagcagaca gataaattag ggctcttagc tgataacgtt gaaactattg
     4681 aacagttaag ctatgagcaa tgtgccttga tggagcgagc aatagcagct aagagaaaag
     4741 aattagataa gaagtgatat gagtgtttaa accattaatc gattcatatt cagcggttct
     4801 gaaaaagttc aaaggaaaag acataagcgc aaccatcaat gaggaagtga acattgatcg
     4861 actaaagacg atgtatgacg gctacgatgg tgatcgagtc attgaaattc gttttattga
     4921 tcctagacgt ttcaccgtac agcaacgaaa cttcatctat gcgctgatag gcgatatttt
     4981 tatcgataca ggcatgccaa cggacttctg gaaggaattc ttctactttc gttttgaagg
     5041 tgtcacaggg cgcaaaataa gcctgaaaga cgaatcgaat acaactgtga gtgatgccaa
     5101 tgtcttagca aatatcatct tagatttcat ctttgaacat catattcctt tcaaagaagg
     5161 ctatgagatt ttacctgcga atcaagaata ttacttctac aaatgcatca caaaaagagt
     5221 ctgctgcatc tgtggcaaaa caggagctga catcgatcac tttgacaaag cgctaggaag
     5281 acgaaagcgc aaagaagttg atcatgcaga gtacacattt gcagcactct gcagaatcca
     5341 tcacacagag aagcacaaaa taggtgtgat taatttcaaa aataaatatc aaatcaaagg
     5401 gatcaaatta aaccagaaaa caatcaaaaa gttaaatatt ggagggtaaa aatgacagaa
     5461 catcgaagtt attatgcgat tataccagcc aacgtaaggt acgacaaaag acttaaacca
     5521 aatactaagt tgttatacgg agagataacg gccttgtgta atgaaagagg cttttgttgg
     5581 gcaggcaatg agtactttgc agatttatat ggtgtgaata aagagaccat atcgcgatgg
     5641 gtaagtgatt tgattaagtt tggatacttg aatcgggaaa tcatttacaa agagggtacc
     5701 aatcaaataa tcaataggta cctacgaatt aatcaatacc ctattgacga aaaacgcaat
     5761 acccctattg acgaaaaagt caaagataat aatacatcta ttaataatac atttaataat
     5821 acaaaagaat atataagaga gttaccgcct tcgaaaaaat cgaaggctaa gcccgtccgt
     5881 cataaatacg gagagtataa aaatgttctt ttgtcagatg agcagatgga gaaactcaaa
     5941 acagaattcc ctaatgatta ccaagagcga atcgaacgac tgtcagagta ttgtgaatca
     6001 tctggtaaga cttataaaaa ctatttggca actattcgaa gttgggcaag gaaagaaaaa
     6061 agtgaaccta agaacgcaag cagtggatac aagcgcacag gaagacgaga gaagcttcct
     6121 gaatgggcaa tcgaccaaga agcctatctc aagaaaaaag cgctagaacg agctaataga
     6181 caatcaaaag caccattcta agaggtggaa aaatgaagat cgattatcta gaactaatta
     6241 atgaaatagc gaattataaa aagggcgagg aattagacgt cctgagagac gtatatgatc
     6301 aactcgaaga agctggaatt gaaggaatta agaatgatcg ttcgagttgg agtaaactca
     6361 gatactattt cgcactctat atcgatggaa cgcaattaag aaatttagca tataccaaat
     6421 tactattcat tgattgtgtt aaaggcttgc aaaaacatct taatgaactt gagcaggtgt
     6481 aaacaagatg gacctaaaga catttacagc acagatcgaa ttaatgcatc aagaagcttt
     6541 aagacaaagt gtgtcgtacg aagacaagtg gctcaacaca tttcatggcg gacgtgagag
     6601 cgcacttgat caagtactca aattactgaa aggagaatgt cgggatggat aagaaagcgg
     6661 caatgcagcg aattatcgaa ttgacttatt cagaagattg gcaggaagat aaaaaaatag
     6721 ttgcagaagt ccaaaagctc ggtaaatcaa tgtggactga aaagcccaaa cggagaacgc
     6781 cgagaaagat tgcaatctgg catggtgatc gaattctagt aacgggtacc gctaaacagt
     6841 tatctgaaat tactggtctg agcaaaaata ttatttggga tagagctagg agcttatgga
     6901 ttgattcaaa aggaagacag tttaggtatg tggaggagaa ataatggacg aactaatcgc
     6961 gaaagtagag cagtgggcaa aagataaggg attggatcaa gcggatttca gcaagcaaat
     7021 gttaaaaacg gtcgaagaaa tcggggaagt tgccgcttct ctagctagaa aagatgaaca
     7081 tggtttaaga gacggaattg gcgatgtagt agtaaccttg atcatcttgg caatgcaaaa
     7141 tgatatggat ttatacgagt gcttggattt tgcttatgac gaaatcaaag gtcggactgg
     7201 aaagatggtc aatggtgttt tcgttaagtc cagtgacctg aaataattcc attaatggaa
     7261 gaaataactg aataggagga tagacatgat tgatgcaaaa caagtgcaaa aacagaaaga
     7321 tggaatgttg atgtttgaag catatgttct accatttctt aatcaatttg aagttttgga
     7381 atgctctgcc agcggagaag aattggagta tgttgtaatt cgtgaaacca aagaaaacgt
     7441 tcagaagcta aacgaatttc tttgcactat taactgttgg gatatgatag ctccaggatt
     7501 tttatgtcca gctatgggag aatttttgga atattgtcgt cttgaagatg caggcacttt
     7561 ggatttagct tatctggtat acaactacct aaacatcaac acagatcatt tgtggtttgg
     7621 cactgccgaa agaaaatggg tagtccgcta accaccaata tcaatgaaaa aaggcggttt
     7681 cctgaaagtg gaattgccgc cattgctgta aggttcttta cgcgactcaa ggtcacagaa
     7741 atataaatgc aaatctcttt tgaagtgatc caaagctctc ggaaaatgga agttttttgt
     7801 ttcaaagaat aaatgagatt tgcgtcaaga acctgagagt tatttccaaa aaagcttcca
     7861 aagaaccgaa tgagctaagg acatcatgtg taaacacata atatagctct cctttcacgg
     7921 ctaatcataa catgaaaaat attcgatgtc agtaggaaaa aaggtgaatt atgaaaagaa
     7981 tattaaatta tcccggcagt aacagattct gcaattaaac tccgaagctg atgtatcagt
     8041 atcaactgta gaatattatg agcgtggaga aagtcaagca gtcacaaatg taaaaatcga
     8101 taacgtcaac ggtcttatct gtttagatga aggcgatagt atgaatgaaa ttgctgcaga
     8161 ccatgaatta tcagaaactg ctttaattga tcaaatcgaa ttagctagac aatactttgt
     8221 tccatatgca gatacctggt ccaagcataa gaatgacatc atttttccag aggaacaatg
     8281 agagcaactg atccagtaat tatccttgag gaagccaaat ttatttggac tcacgaagag
     8341 atagagcaag cacgcttgct cttttctcaa ggagttaagc cgagcaaagt agctgaaata
     8401 atgggtcaaa agattcttga tgtcggattg cttttgctcc atctagcaga aaaaaatttg
     8461 atttgaggtg aaaatgatga ttcaacttgc aggcatccaa acagggaaaa tttatttttc
     8521 tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa acttacacga acaataagaa
     8581 gctaaggaaa aaatatcctg atttgttgct aaaggatgat cagattatgc cggaaccgat
     8641 gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc agccgtgtgg gctggagttg
     8701 ctatttcttt ttaataatga gtttttctaa atttaattca ttctc
//
LOCUS       DAJSVD010000112         8527 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSVD010000112 DAJSVD010000000
VERSION     DAJSVD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.114"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(218..895)
                     /gene="nth"
                     /locus_tag="ON498_002373"
     CDS             complement(218..895)
                     /gene="nth"
                     /locus_tag="ON498_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3940601.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(902..1600)
                     /locus_tag="ON498_002374"
     CDS             complement(902..1600)
                     /locus_tag="ON498_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3940602.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1868..3052
                     /locus_tag="ON498_002375"
     CDS             1868..3052
                     /locus_tag="ON498_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR3940603.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3058..3501)
                     /locus_tag="ON498_002376"
     CDS             complement(3058..3501)
                     /locus_tag="ON498_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3940604.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3727..4014)
                     /locus_tag="ON498_002377"
     CDS             complement(3727..4014)
                     /locus_tag="ON498_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940605.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4185..5537)
                     /locus_tag="ON498_002378"
     CDS             complement(4185..5537)
                     /locus_tag="ON498_002378"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR3940606.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5810..6256)
                     /locus_tag="ON498_002379"
     CDS             complement(5810..6256)
                     /locus_tag="ON498_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3940607.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6492..7418)
                     /gene="cysK"
                     /locus_tag="ON498_002380"
     CDS             complement(6492..7418)
                     /gene="cysK"
                     /locus_tag="ON498_002380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3940608.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7558..8445)
                     /locus_tag="ON498_002381"
     CDS             complement(7558..8445)
                     /locus_tag="ON498_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3940609.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2321 a         1750 c         1417 g         3039 t
ORIGIN      
        1 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
       61 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      121 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      181 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      241 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      301 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      361 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      421 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      481 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      541 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      601 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
      661 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
      721 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
      781 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
      841 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
      901 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
      961 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1021 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1081 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1141 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1201 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1261 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1321 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1381 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1441 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1501 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1561 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1621 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     1681 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     1741 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     1801 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     1861 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     1921 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     1981 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2041 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2101 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2161 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2221 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2281 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2341 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2401 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2461 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2521 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2581 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     2641 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     2701 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     2761 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     2821 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     2881 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     2941 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3001 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3061 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3121 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3181 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3241 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3301 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3361 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3421 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3481 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3541 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa
     3601 aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag taaagagaca
     3661 gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc ttttcaaaaa
     3721 ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat atgtgtactc
     3781 tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag gaaaacgatg
     3841 agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt gcgtttcatt
     3901 caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt tcttcgaagc
     3961 acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat tcatcttctt
     4021 tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa cagctttatc
     4081 tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact tttcacaaaa
     4141 aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg tgcagtttcg
     4201 atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg gtcaaaatgt
     4261 ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt ttgaacagct
     4321 aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg agcacctacg
     4381 atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt agttggcatg
     4441 aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc gttcaaagtt
     4501 gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta aagtcctgaa
     4561 gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc cacaagcaaa
     4621 ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact tggattgtag
     4681 ttgactactg cagagtctcc agctgcaaaa atgtctggat tacttgtctg catgtattca
     4741 ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag ttttgtattt
     4801 ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt tggtgtagct
     4861 acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc gcctagcgca
     4921 agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg tttatctaga
     4981 tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc agattcgaca
     5041 aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac acgttttgct
     5101 tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag gagtacattt
     5161 ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt catcactaat
     5221 ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt tactgtttta
     5281 ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc taatgaagat
     5341 aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc gccgacataa
     5401 agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac tgtcacttct
     5461 gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg agtacagcca
     5521 attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg acaaaactac
     5581 tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa aggtttgttt
     5641 gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt attttaataa
     5701 tattcacttt tatatctaag attgtgaata aaagacaaaa aaataaaaaa gctagcgttt
     5761 tttttacaca gctagctttt tttaggtata acacttgtag aaactgtttt tacatttgcg
     5821 ccttttttgc tgactcttta caatccggac agagtccata caattccaac cgatgttcat
     5881 taatctcaaa atcagtcagt ctgcttgcag ccatttctat atcttcaagc cctgggtaat
     5941 gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc tttgaactga
     6001 aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca atcgaagtaa
     6061 ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga tgttcaagtg
     6121 ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat tctaaaaccg
     6181 cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata acctcttcga
     6241 ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca tactattttt
     6301 acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt ttaatgaaaa
     6361 aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt tttctcatca
     6421 taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta tctggagaaa
     6481 aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc accattgtcg
     6541 ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt tagcgcggca
     6601 gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc aacttctcta
     6661 gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac agaagtatcc
     6721 agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc cgcttctttc
     6781 ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt cggatacaca
     6841 cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc tgcaacaaac
     6901 gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt cttctcatgc
     6961 acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt ttcttttgcc
     7021 aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt caaaatcaac
     7081 tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc aggcatcacg
     7141 atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc tgtatttcca
     7201 gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc tgctttttct
     7261 atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa ctcaagcttt
     7321 acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat tggcgttttg
     7381 ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc cttgtctttg
     7441 ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg tctttgacta
     7501 gcaaaaaaaa gacaaagata taccatatat ggtaatttct ttgccttttt atctaaatca
     7561 aacgtttagt tcttgaccaa ctaataaaga ataactggac atagacatgc catttttcgc
     7621 cattagatca tcaacagaca tgccaaattt ttgtgcaata tcccatagcg tatcacctga
     7681 cacgactgta tatgtctgac cattatttgt atttacatct gttgttgaag atgatgacgt
     7741 tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggtt gttgtagccg aacctgttga
     7801 tggcgtatca aaacgggtta aattataagt attaatcaat gagttcaatt tggccccata
     7861 atttggatcg gtagcataac gacctgtcaa gtacgctgtt gcatcataaa aacttgttgt
     7921 attgcttttc cacacgccag cataatgata atcgcccgtg gaaaaactgg ttgaacgaag
     7981 aacattcgca tggtcttgga aggattccca ataagaagaa tattttcgga atggctcatt
     8041 catcgttacc cattgtccgt ttacatattc tttggttgcc atataaacga cctgtccgtt
     8101 atagcttcct ttcacaccga atagattata atttggcgca ctagctaatc cggaagtgcc
     8161 atatgcactt tccaacaacg cttgagcgat catgactgaa gcatacaagt cattacttgc
     8221 agcaacttct gacgcagacc agccgatttg atcgataaac gcttgaggat cagcgactgt
     8281 ttgagatcct tgttcatctg catgaacatg ttgcggtacc gctgtaacca tcatcggccc
     8341 aactaaaaga ctcgttccaa ttaaaggagc aatcgctttt ttattgatca ccatttttct
     8401 tttaggtcta tggtgccggg cactgcgggt cttcagctct tgcattcaaa ttcctcctca
     8461 aaataaaaaa acttaataaa aagttctctt cttaaaacaa acttaattaa ttaaattata
     8521 cttttaa
//
LOCUS       DAJSVD010000113         8494 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSVD010000113 DAJSVD010000000
VERSION     DAJSVD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.115"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..173)
                     /locus_tag="ON498_002382"
     CDS             complement(<1..173)
                     /locus_tag="ON498_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940610.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="ON498_002383"
     CDS             complement(593..1714)
                     /locus_tag="ON498_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR3940611.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ON498_002384"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ON498_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR3940612.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ON498_002385"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ON498_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR3940613.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ON498_002386"
     CDS             complement(4304..5215)
                     /locus_tag="ON498_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3940614.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ON498_002387"
     CDS             complement(5232..6239)
                     /locus_tag="ON498_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3940615.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ON498_002388"
     CDS             complement(6236..8365)
                     /locus_tag="ON498_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3940616.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="ON498_002389"
     CDS             complement(8370..>8494)
                     /locus_tag="ON498_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3940617.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAJSVD010000114         8349 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSVD010000114 DAJSVD010000000
VERSION     DAJSVD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.116"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            6..293
                     /locus_tag="ON498_002390"
     CDS             6..293
                     /locus_tag="ON498_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940618.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            709..2247
                     /locus_tag="ON498_002391"
     CDS             709..2247
                     /locus_tag="ON498_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR3940619.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2244..3209
                     /locus_tag="ON498_002392"
     CDS             2244..3209
                     /locus_tag="ON498_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940620.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3196..4518
                     /locus_tag="ON498_002393"
     CDS             3196..4518
                     /locus_tag="ON498_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3940621.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4581..4685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4759..5502
                     /locus_tag="ON498_002394"
     CDS             4759..5502
                     /locus_tag="ON498_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940622.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5517..6509
                     /locus_tag="ON498_002395"
     CDS             5517..6509
                     /locus_tag="ON498_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3940623.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6521..7258
                     /locus_tag="ON498_002396"
     CDS             6521..7258
                     /locus_tag="ON498_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940624.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7248..7970
                     /locus_tag="ON498_002397"
     CDS             7248..7970
                     /locus_tag="ON498_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3940625.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8054..>8349)
                     /locus_tag="ON498_002398"
     CDS             complement(8054..>8349)
                     /locus_tag="ON498_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3940626.1"
                     /translation="EKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTA
                     TNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2716 a         1437 c         1793 g         2403 t
ORIGIN      
        1 gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa atcacccaaa aaaacagagt
       61 atcaaggagt ttttttggct caagaacaaa aactggtgcc tgtgacaatg gtccagataa
      121 ataaagcaaa tcaatttatc tttttatttg aacacggcaa agcaccactt gctatgaaag
      181 acatctttct ttttttgatg aagaaccgtg agaaagctgt ttattatcag tcaaaagaag
      241 aactcattcc tctttatgga gtgaaagaga tagaaaataa agtggtgata taagtaaaaa
      301 taagaaaaca agacgtttat gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag
      361 tgtcatgata cagtataata ttcgttgaac aggccaataa aggtgttctc ccccacgaga
      421 tttctacttt attggctgtt tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata
      481 tagctgattc caagtgacaa tttcgtatag atttttatgg tattcgtagt gaaaaaacga
      541 gaaccaaaaa gttgatccga ctttgttgct tggattcttt ttttgtcagc atatttagag
      601 gtattttttt cttttttttg ttcgtgctgt aaggttaagg caagacaagt tagttgggaa
      661 aaaggaaaga aatgtttata cttaaagaat aacagaaagg aaggtcgcat gaataatttt
      721 atacaaacat taagtgaaag aaaaggcgag ttgctggaag ctgcatttca acatctgtcc
      781 atttctctca tttctttgct gattgcagca ctgattgcta ttcctttagc gatttgggca
      841 gccaatcata aaaaaatggc agaagtctta ttgcagatca cgggtgtgct ccaaacgatt
      901 ccttcacttg ccttattagg tttacttatc ccatttgtag ggatcggaac tgttccagca
      961 ttgattgcac ttgttattta cgctttgctt ccgattttcc aaaacacgta tattggtcta
     1021 gctgaaattg acccgtctat tgaagaagca gcggtagcat ttggtatgtc cagaatgaga
     1081 cgcttgctga aagtagagct tcccatcgca ttacctgtca ttatctcagg tatccgaact
     1141 gccttagttt taatcatcgg tacggctact ttggcagcat tgatcggagc aggtggatta
     1201 gggacattta ttttgttagg tattgaccga aatacacctg ttttgacatt gattggtgcg
     1261 atcagttctg ccttgcttgc aattatattc agcggactga ttcgctggtt gcagcataag
     1321 aaacctcgat attccatcat tactttagca gcagtggtgt gtttatttgg cggcgtttct
     1381 ttatatcagt ctgccttgtt taaaaatgaa accatcacga ttgctggaaa attaggagcg
     1441 gaacctgaaa tcctgatcca aatgtataaa gaattgattg aagacaacac aaaaacagaa
     1501 gttgaattaa aaccgaattt tggcaagacg agttttttgt tcaatgcact ggatcaaggt
     1561 caaatcgata tctatcctga gttcacaggt actgtcctag aaagcttggt gaaggtacct
     1621 gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc agaagaatta
     1681 ttgaataagc aattcgatat gacattatta aaaccaatgg cttaccaaaa tacgtatgcc
     1741 ttggcagtca agagatcttt tgcgaacgaa catcaactta aaaagatttc cgatttggca
     1801 gctatctctg atcagatcaa agcgggattc acactggaat ttattgatcg ttcagatgga
     1861 tataaaggaa tccaagaagc atatggcttg aatttttcaa gtgtacaaag catggagcca
     1921 agtttgcgtt accaagcaat caataatgga gatgtcaata ttgtggatgc ctattccaca
     1981 gatagccagc tcaaggagta cgatctagtg actctagaag atgatcgagg gttattccca
     2041 gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt
     2101 aaagcactga ataaattaag cggaaaaata acagaggatc aaatgattga tatgaattac
     2161 caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa
     2221 ggattagtaa aggaggaaga tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa
     2281 agtatgatgg aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa ggagaaatct
     2341 ttgttttggt tggtccttcc ggtagtggaa aaacaactac tttgaaaatg atcaatggat
     2401 tatctaaacc atcagcagga gacatctact tcaaaggaaa atcgttaaat gaatataatc
     2461 tgcagaaaat gcggtggaat atgggatatg tgctgcagca aatcgctttg ttcccgacca
     2521 tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa
     2581 gagcagaccg tgtagatgaa cttcttcaaa aagttggctt atctcctgat atttatcgag
     2641 atcgtatgcc tagagagttg tctggaggag aacagcaacg gatcgggatc atccgagcta
     2701 ttgctgcaag cccagatgtg attttgatgg acgagccatt tagcgcatta gatccgattt
     2761 caagaaactc attacaagaa ttggttttgt ccttacacga agaattgggc acgaccatcg
     2821 ttttcgttac gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt gcatttatga
     2881 aggatggaga aattatccaa tgtgacacac cagagcagtt attgatgaat cctaaaaacg
     2941 actacgttcg gcatttcttt gatgaaccga aacagacgaa agagtggcga gtagaagatt
     3001 tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct
     3061 ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt gtatccaagc attgtgatag
     3121 tagaaaagca aagaagtatt gggtcgctga caagtaaaga aatctttgct tttttaagtc
     3181 gagaggagga aggaaatgaa aacatatgat gcaatcatca tcggttcagg tgtgagcggc
     3241 ttaagtgctg cttatggatt gaaagaagca ggtaaaacag tactagtggt cgaagaagat
     3301 ctatggggcg ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt gttaagtgca
     3361 gtagaggcac gtaatcgggt aaaacaattg agtggaaaag gttttaacga gatcccaact
     3421 gccaactggg aagaacttca aaaattcaaa cgaacattta cggatccagt cccagaaagc
     3481 cgaaaaaaac aattagcaga agctgaaatc gatcatcttt caggaaccgc tcgttttttg
     3541 gatgattcat ctattgaagt caacgaagaa gtcttccatg cggattactt agttttggca
     3601 accgggcaaa gacctacgat cttaccagtg gagggaaagg aatacttgaa aactagtgca
     3661 gactttttat cattgcctgt actgcctaaa gaaatcattt ttatcggtgg tggttatatt
     3721 gcttttgagc tagctacgat tgccaatgct gcagggagca aagtaaccat tgtccatcat
     3781 aatcaacggc cattaaaaga atttgaggcg tctttggtag aggaagccgt ccatcaaatg
     3841 gaagcatctg gcattcagtt tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt
     3901 actcgctatc gtttagtcgg aaaagagacg gaattagtag cagatatgat tttttgtgcg
     3961 actgggagac agccgaatac agaatcttta gcacttgagc aagcgaatat cgtctttgat
     4021 aaacacggaa ttgcagtcaa tgattatctg caaacgagca atccaaaaat ttttgcgtgt
     4081 ggagatattg tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat
     4141 tatgtagcta agcggataac ggatgcaaca agcgaaccta tcaaatatcc gattatacct
     4201 acgatcgtct acgccagtcc taaacttgct gaagtaggag ttacaaaaag tcacgcctct
     4261 tcgtcagatc aagtggtaga gatggatctt accagttggt tcacctatca tcgtgtcaat
     4321 gaaccagttg ctaaggctga attgaccttt gaccagcaaa attatttgat tggtgctgca
     4381 gtgattagcg agcaagcaga cgaattgatc gatgatttga cactcgtcat caatcaaaaa
     4441 ttgactaaaa aagagttgga tagctatatc atggggtatc cgacattggc aagtgatctc
     4501 tcctatttat tgaaataatc agcatgataa ctaaagcgct tgttgctttt tccttcttgt
     4561 gctacacttt aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca tgagagtaag
     4621 ttgtgaactt agacccattg aacctgtcag ttaatactgg tgaagggaga aacatagcag
     4681 acgcttgaag cgggttacta tgtcgtgccc atttcaagcg ttttttgtta ttcatattag
     4741 aagaaaggag gtaacaaaat gaagaaaaat ctttatccag cagtcagtat ggcattcatt
     4801 ttaattattt ggcagattgt tgtcatagtt tggaaaatac ctgcctttat tttacccgga
     4861 cctgctgctg tattttctgc attagcggca gatgcacaag tattactttc ccattctgtc
     4921 acgacattaa atgaagcagt gcttggtttg gtaatagcta gcgttctttc ttttattact
     4981 gccttggcaa tggattattg gaagttttta gaaatgtctt tttatccgtt actagtgatt
     5041 tcacaaatgt tgccaatcat ggtgttaggg ccattactta ccctatggtt tggtttcggt
     5101 atgcttccaa aagtgatcct tgttgtcttg ataagctatt ttccgatcgt agtttctttt
     5161 tcggatacat tgaggaaagt atcaaaagag caaatcatct ttttgaaaac gatgggggca
     5221 gatacaggga agatttatcg aatatataaa ataccagcgg gtttatccgg cttcttttcc
     5281 ggtttaaaag tggctgctac ttattgtgtc agcggagcag tcgttggaga atggctaagc
     5341 gctcaagcag gcttagggta ttacatgata cgcgtaaaaa acagctacca gatcgataaa
     5401 gtttttgctg cgatcatttg tgtcattcta ttgagtttat tgctgaatgg cgcttgttca
     5461 ttattgaaaa aagggtatta tcgtatgctt tatatttttt aagagaaggg gatcaaatga
     5521 aaaaattatg gcttttatta ccgctactgt tgcttttatc cgcttgtggc actgcaaaag
     5581 aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt ttggattatg
     5641 taccgaatac taatcatact ggcatctacc tagcaaaaga aaaaggctat tataaagagg
     5701 ctggtttaaa tgtccaaatc atcgaaccag gagataattc gacaagtatt ggtctagtag
     5761 gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag
     5821 atggacagaa tataccagtc aaagcaattg ctacagtgat caaacataat acttctggat
     5881 tcgccaccct atcagacagt aacatccatt caccaaaaga ttttgaagga aaaacatatg
     5941 caggttggca aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg
     6001 gggacttttc aaaactgaca atggtaggaa gcaatggaga agggccagaa agcttaggaa
     6061 aatcctcaga tatccaatgg tactttgaag gttgggatat gatcaaagca aaagaagcgg
     6121 gtattgaact aaactatatc ccattgaaag aattagatga acggttagat tactatactc
     6181 cggtaattat tacgaatgat caactgatca aaagtgatcc agaattggta cagtctttta
     6241 tggatgccac gaaaaaaggc tatcaggaag ctattaagga tccaaatgac agtgctaaac
     6301 tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt agaagaatca caagcattct
     6361 tgtcaaaaaa ttatacggat gatcctgaga attggggatt gatggaagaa aaagtttggc
     6421 gcaattatac agcttttatg caggaaaacg gattgatcaa gcaagatgta ccttcgcaag
     6481 aactattcac taatcagttt ataaaatagg agtgatagat atgtcaatcc aaatcagcca
     6541 cttgaatgta gtcattgaaa acctgaaaat attagaagac atccatttca ctatccctaa
     6601 agataccttt acatcgattg tcgcgccaag cggagccggt aagtcaacat tgctgaaaac
     6661 tttaacagga gtccagccta tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac
     6721 tggtctccat acagcattta gttatatgcc gcaagaagat atgttacttc cttggctgag
     6781 cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat ctaacaatca atgaagaaca
     6841 agtaagggat tatctggata tctttgggtt aaacggctat gaacattttt tgccggaaca
     6901 attatctggc gggatgaagc agcgaacggc cttgttacga acaatcatga atccttcttc
     6961 ttacttgctt cttgatgaac catttggagc gttagatgct atgaccagag ggcagatgca
     7021 agactggatg ttgaagctgc ctaaaaaagc aaagcggact actttacttg tgacgcatga
     7081 tatcgaagaa gcaatttatt tatcagacag gattcttgtg ctttccgccc gtccggcaca
     7141 tgtgattgct gagattcaag tgcctgaaaa acgacggtca cgtgaatggc tactgcagca
     7201 atcagagttg aagcaaacga tttatcagtt attggcaggt gaattagatg ttaagtgatg
     7261 ctcattgtca tttagatggg agtcagagtt tagcactcct tcagcaggaa cacacgattt
     7321 tgacgatcat caattgtgat tcgccagaag aatggaagga aaacagacag cttgcagcaa
     7381 gtaagactca agccttaagt tacggtatcc atccttggaa agcagatagc tatacgtttg
     7441 aacaagttga gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg ttagataata
     7501 tttggacgaa tgtaccgatg actacacaaa aaaaagtatt tgagcgacag cttgcttttg
     7561 cagccataaa tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa
     7621 attatatcca agcttaccct aatcactatt tgatccattg gtattcttct ttggactatc
     7681 aacaagacta tattgattta ggctgttatt tcagcattgg tcttgatttg aaaaagaatc
     7741 cagcagtctg gcaactagct aaagcagtcc caatcgacca cttacttatt gaaacggatg
     7801 gactacatgc agcagagtgg gctttagggg ggattttttc agaaaaagat tatgtctcac
     7861 tgcttgaaga acagttaaag acaatcgctg agatcaaaga gctatcagtt gaagaagtta
     7921 aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt aggtaaataa aaaagagatg
     7981 tgacacaact agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa actgtttgat
     8041 taataaatga atcttagtac caaccgtttg cttcccagaa tgctttcgcc gcttcccaag
     8101 aaccataacg tgaagtaaca tattgttctg ctactctttc ttggtttgca gcagagtagt
     8161 caccatttaa gtaagaagaa tctaattggt aacgtccgat gtaacgtccg ttcgttgctg
     8221 tgtaagaacc gcttgattct ttttgtgcga tccattcttt tgcagagctt gtgcttgctg
     8281 gagtagcagc agtttctgtt acagcaggag caggttgtgt ttcagcaaca ggcgctgctt
     8341 gtttttccg
//
LOCUS       DAJSVD010000115         8139 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSVD010000115 DAJSVD010000000
VERSION     DAJSVD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.117"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            162..995
                     /gene="msrA"
                     /locus_tag="ON498_002399"
     CDS             162..995
                     /gene="msrA"
                     /locus_tag="ON498_002399"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3940627.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1108..2064
                     /locus_tag="ON498_002400"
     CDS             1108..2064
                     /locus_tag="ON498_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3940628.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2353..3051
                     /locus_tag="ON498_002401"
     CDS             2353..3051
                     /locus_tag="ON498_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR3940629.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3201..4517
                     /locus_tag="ON498_002402"
     CDS             3201..4517
                     /locus_tag="ON498_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940630.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4534..5949
                     /locus_tag="ON498_002403"
     CDS             4534..5949
                     /locus_tag="ON498_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3940631.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6413..6733
                     /locus_tag="ON498_002404"
     CDS             6413..6733
                     /locus_tag="ON498_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940632.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6759..7076
                     /locus_tag="ON498_002405"
     CDS             6759..7076
                     /locus_tag="ON498_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3940633.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7105..>8139
                     /locus_tag="ON498_002406"
     CDS             7105..>8139
                     /locus_tag="ON498_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940634.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2683 a         1321 c         1725 g         2410 t
ORIGIN      
        1 ttataaaata aattgattaa aaaggagaat ctgtcgataa ggattctctc ttttttatac
       61 gaaaaatctg tagggggatg aagtaatcgc ttatttcctc actttatgat aaattacagc
      121 taaagtcact agcttgtctt cttcggaaag gaggagaatc tatggatgag gaacaaatat
      181 tcattcaaac actttataat ctgattttga atccgaatac acgagattgg gaacgaaaag
      241 tattgattca gacaaagaat gacataagag aaaatatatc cgttaaagaa cagttatcaa
      301 aactagaagc aacactacgt ccattagcta tccgaatgaa tctgacgcct gatgtaatgg
      361 acttttattt attgattacg gaaggatttg ataaggagca aaaatatgac ttttccaagc
      421 atgccatgca agatgctgac tatcaagaac gtgcagtatt tgcaggggga tgtttctggt
      481 gtatggtcga gccgtttgaa tcgaaaaaag ggatcctatc tgttttgtca ggatacacgg
      541 gcggacatgt tgaaaaacca aattatgatc aagtaagtgg tggttacaca ggtcatgtgg
      601 aagctgtgga gatcatttat gacacaagag aaataagtta tagcgagttg ctgacaatct
      661 attggcaaat tactgatccc acagatacat ttggacaatt tcaagataga gggaagcaat
      721 atcgtccagt gattttttat caagtcgaac gacaaaaaga attagcggaa caatcaaaac
      781 agaaactaga cagttcagga actttccacc aaccaatcgt cacaaaaatc gaaccagccg
      841 gtacgttttg gccagcagaa aactatcatc agcaatttta caaaaaacaa ccgaaacgtt
      901 ataaaaaaat ccagcaagca cgtaatcaat ttctgatcta tcaacgagta aaaaacaaat
      961 ggcaaaaaaa tatacgaaaa aatcattttg attaactcct tttctttaaa atgtagtaaa
     1021 ctaaaaatag aaaaaagaaa gcgttttaaa aagatttgat gaggaggaga aaaattgtat
     1081 attgcagata gtgcacgtta tgaaaaaatg gaatatcgta aatccggtca ttcaggtttg
     1141 aaattaccga ttttatctct cggattatgg caaaattttg gtgattatga tcctatccat
     1201 aatcaaagag agattttaag aggcgcattc gacatgggaa ttacccattt cgaccttgcg
     1261 aataattatg gaggccctgc tggagctgca gagaaaaact ttgggcgtat ttttagagaa
     1321 gattttcaag cctaccgtga tgaactgatt atttctagca aagcaggtta tcatatgtgg
     1381 gaagggccat atggggaatg gggctctcgt aaatccatca tttcaagttg tgaccaaagt
     1441 ctccaaagaa tggggctaga ttatgttgac atcttctatc atcatcgccc agaccctgat
     1501 acacctttgg aagaaacagc agaagctttg atgcaacttg taagacaagg aaaagcatta
     1561 tatattggta tatccaacta taatggagaa gatacaaaaa aaatgactga aattttaaaa
     1621 agaaaggaag caccgttcat tattcatcag atgcgctaca atatgttttc tcgtgcacta
     1681 ctagaagacg atctatctcc agtattagaa gaagaagggc taggggcaat cacgttcagt
     1741 ccgcttgctc aagggttgct gacaaatcgt tatctacatg gtattcctga agattctaga
     1801 gcacatcgta aggaaatacc attcctttca gaagaacaag ttggatcaac tctggaaaaa
     1861 atcaaagcac tgcaaacaat cgccgtatca cgaggacaat cattagcgca aatggccctt
     1921 gcatggaatt tgcgacaaaa atctgttaca agtgtactgg tcggagcaag tcggttaagt
     1981 cagttacaag aaagtgttcg aatgatggat aatttagatt tctcaccaga agaagaattg
     2041 agaatcgacc aaattctaga atagaaacga tgtaatcaaa ctttttatcc atatattaaa
     2101 aaaaacaaaa gatgacatca agaatcgaat gaagatcgtc tgttattgaa catcatattt
     2161 tttgtcgttc aaaataaaaa tgatcgaagg ctaggagtga attttatcct agtctttttt
     2221 atggtaaata tcttcttcgt ttgtatgtct atgaacggat taatcaattt tgtatacaaa
     2281 aaatcatcag atgattcaaa cgtgttataa taattacgaa aaaatacatc agatgaaaag
     2341 gaggtatgtg gtttggaaga aaataaaacc ctagtagaag taactactga caagatcctt
     2401 agatatttaa aggaaatgca gttgcaagta ggagataaac tgccgaatga atatgaattg
     2461 tccgattatt tagaggtggg gcgaagcacg gtgcgagaag ctgtgcgggc tctagcttcc
     2521 aggaatatcc ttgaagttcg gcaaggagcg gggacatttg tcagtgataa gcgagggatt
     2581 gccaatgacc cactcggttt ttctttgatt gaagatcagg atcggatgat acgagactta
     2641 tttgaactga gatatttatt ggagcctaag atggcaggtt tggcagcgga atatgcaacg
     2701 aagaatcaaa tcgtccaaat gaaaaaatta acgcaaaaga ttgaaacttc ttttcgagaa
     2761 ggcacacaag aacatgttca attagacatc gattttcata cgatgattgc agaagctagc
     2821 ggaaacgtag ccttgctgca tattatccca atcattaatg aatcaattgc tttgtttaat
     2881 aaaaattatg atatccagca tctgaaaatc gagacgatcg atattcatac agagatcgtt
     2941 caggccatcg aaaggcggaa tgcacttgct gcgtttgatg caatgactgt ccatatggca
     3001 ataaatcgga gagaattaca gaaaataata ggagaaaaag aaactaattg acagttcatc
     3061 tgatgtattt tatgataaat acatcagatg aatttttgtt ttatatttgg taaaaaagag
     3121 gagtaaatag tttctgttca tcgaaatgta agcgctgact aatagacaaa aagagatgac
     3181 gtcaaggaga agaagcagga atgaggggat ctggaatgag ttttatggat aaattagcca
     3241 atgttttagg gaacgtggca acgagaatca ataatttaag gtatatcatg gtgataaaaa
     3301 acgcatttgc tgcattgatt ccggtaatta ttacgggtgc ttttggcaca ttgttttcag
     3361 caatggtctt tgactcggaa aatggcttag cacagataag tttcttgcgt tttttagaag
     3421 aattaaagcc tatcgcatct gccatcagct atgtcacact aagtttcttg accatctatg
     3481 ctgtttttct gatagggatc gaattagcca agctgaataa agtggatggt gtattccctg
     3541 gtatcatcgc agtgatgtct tatttatctg ttaatccaac tgtttatgaa ttagtgaatg
     3601 aaaatcagac tgttatagta gaaaacgtat tggctaaaca atatacagat acgaaagggt
     3661 tatttttggg aatgttggtc tccatccttt ctattgaact ttattgctgg ttgaggaaac
     3721 agaagaaact acagctgaga atgcctgata ccgtgccaac gaatgtttct gctagttttt
     3781 ctgctttatt cccgacgatt ctgactgtat cgggtatcgc ggctgcggga tttatcatca
     3841 aagaacttac aggaatgtac ctttatgata tcatttacaa tgtcgtacaa cgcccattag
     3901 aaggtgtcgt acaagggtta ccgggtattt tgctattgat gtttattgcg caagtctttt
     3961 gggtgattgg tatccatgga aatcaaatgg tgaagccgat acgggaaccg ttgcttttag
     4021 cggcaattgc tgtaaatacg gaagcttttg aagctggaaa agaagtaccg aacattatta
     4081 ctatgccatt ttgggatatg tatatgagta tgggcgggtc tggagtcacg atcggtttac
     4141 tgattgcagt attgttagtt ggaaaacggg aagatatgaa acaaatcacc aagttatccc
     4201 ttgctcctgg gatcttcaac atcaatgaac cagttatttt tggtatgccg atcatgctga
     4261 atccaatttt agctattccg tttatcttaa cgcctcttat cactggaacg attggttatt
     4321 ttgcaaccgc tttaggattt gcggcgaaag cagttgtaat ggtcccttgg ccaatgccgc
     4381 cgatcgtcaa tgcttatctg gcaacagcag gaaatatcgg tgcagttatt acgcaacttg
     4441 tttgtattat tgtttctatt ttgatctatc tgccatttgt aaaagtagca aatcgtgtgg
     4501 aaaatgatgg gatctaaaaa acaggaggaa atcatgaaaa ttactattcc aaaaaatttt
     4561 attcttggtg ctgcatcttc tgcttggcaa acggaaggtt ggaaaggaaa aaaagaagga
     4621 caggattcgt ttatagatag ctggtataaa aatgaacatt ttgtttggca taatgggtat
     4681 ggcccagccg tagcaactaa ttttatggaa caataccagc aggatgtgga tctgatgaaa
     4741 tccatccatc tgacacatta tcggacttct atcaattggg ctcgtttttt tactgattat
     4801 gagaactgta tagtagatga agaatatgct cagcatataa gtgaaatgat cgactctttg
     4861 atcgaagcag acgtagaacc gatgatttgt ttggaacatt atgaagtccc agcttttctt
     4921 ttggaaaaat atgatggctg gtcttcaaga aaagtagttg atttttatgc taagtatgct
     4981 caaattgcct ttgatcgtta cggtgatagg gtaaaaaact ggtttacatt caacgaacca
     5041 atcgttcccc aaacgcgttg ctatttggat gctgttcgct ggccgcatga acaaaacaca
     5101 aaaaagtgga tgatgtggaa ttatcataaa gttttagcaa cggctaaagc agtagagatt
     5161 taccgcagcg gaaattatgg tggaaggatt ggctgtattt taaatcctga aatggtctat
     5221 gctcgatcca attctccaga agaccaaaaa gctgcttata tgtatgattt gttttataat
     5281 cgagtatttt ttgatccgat ggtaaaagga tactacacag aagagttcct ggagttgtgt
     5341 caaaaacaat cgatttattt cgatcctgca acagaggatt tagaaacgat tcagcatcat
     5401 acagtagatt tccttggaat caatcaatat tatcctaaac gcgttcgcgc ccctcgttat
     5461 caatggaatg aagagacacc tttccatcct gaacgatatt atgaagtctt tgatttacct
     5521 gggaagaaaa tgaataattc caggggatgg gaaatctatc cgaaaatcat gtatgatata
     5581 gccatgtatc taaaagaaaa ttatcataat atcccttggc tgataacaga aaacggtatg
     5641 ggaagagaat atgaagaaca gtatatggat gagaaaggaa tcgtgcaaga tgattaccgt
     5701 atcgaattta tcggacagca tttgcattac ttgatcaaag caacagaaga aggcgcaaat
     5761 tgcgaaggct atatgttatg ggcattcact gactgtgtgt caccaatgaa tgcatttaag
     5821 aatcgatatg gattagttcg aatcgaattg aacgatgaac gaagcagaac attgaaaaaa
     5881 tctgcttcgt ggtatcagac tttgattgat aagagagaat tggatttgcc tgacgaaatc
     5941 tataaataaa tagaagtttt ttgtagatgt tcattgtatt tactggttat ttgaaataaa
     6001 agaggttgtg aatccagcgt tttgagctaa tacccagggt cagctgattg aacaccgttt
     6061 attgagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaatagcaa agtaaaaaca
     6121 acatcttatg tttttaactt ggttggactt attaccgcag atgcaagctt ttgaataccg
     6181 tttatttgaa aaaagttgtg acaagttttg tgtcacaact ttttttgtgc acgttaaaag
     6241 aaaaaaggta ataaaaatta atttgtgtgt ttagtgtgcg ctttcttttt tagtgtgtat
     6301 gcaacttggt atagacctta aatgtgatga tatcaacttt ttaaagcgct tttaaagttg
     6361 gcacgctact tgcttatata caagtgtaag tatagaaagg aacgtgttta aaatggaaaa
     6421 gaaaacaatc atgcttgtat gttcagctgg aatgagtaca agtctcctag tcacaaaaat
     6481 gcaaaaggca gcagaagcaa aaggaatgga agcagacatc tttgctgtat ctgcttcaga
     6541 tgcggataat caactagaat ctaaaaatgt agacgtttta cttttaggac cgcaagtacg
     6601 ctttatgcaa aatcaatttg ctgaaaagct agcacctaaa ggcattcctt tagatgtaat
     6661 caatatggct gactatggca tgatgaatgg tgaaaaagta ctcgaccaag cagaaaaact
     6721 aataaacaaa taataaaaat tatcggagga tacaataaat ggaagatcaa aaaaatctag
     6781 aagccatcat gggtctgatc atgtttggcg gaaatgcaaa aagcgatgca atggaagcga
     6841 ttgctgctgc gaaaaaaggt gatttcactt tagcagatga aaaaatcaaa gatgcagagg
     6901 aatctttagt ccaagcacat cactctcaaa cagagatgct gacacaagaa gcacaaggaa
     6961 atcacgttga agtcacgctt ttgacggtcc acagtcaaga tcatctgatg acatctatag
     7021 cttttacaga tttagcaaaa gagatcattg atctgtatcg tcgattgaac gcgtaatcta
     7081 cagtaaagaa aagagggaag atttatggat aaatttgttg catttatgga gaaacatttt
     7141 atacctgtag catcaaaaat tggtgcacaa cggcatttag ttgcgattcg ggactcattt
     7201 atggtgagta tgccgttaat gatcttagga gcattagcag taatgatcaa taacttaccg
     7261 attcccgggt tccaagaatt gatgaactca atttttggtg gtgaatcatg gaaaggattc
     7321 ggggctgctg cttggaacgg tacatttgcc attctttctg tcttgattgc tttcttgctt
     7381 gcttatcatt tagctaacgg ctaccgaaaa gacggtgttt ccgcaggtgt gatttcttta
     7441 ggctctttct tcgctttagg cggagcatta ggtatgagct caaacggttt gtttattgct
     7501 attattgttg ggattatttc aacagaaatc tttgtacgtt taagcggaaa tgaaaaattg
     7561 atcatcaaaa tgcctgatgg tgtgccaccg gcagttgcaa aagcatttgc ttcattacta
     7621 ccagcaatga tcactatctc tgcgtttgct ttagttgctg caatttttgc tgcattcggt
     7681 gttgatgata tcgtaggttc tttctataca gtggtccaag aaccatttat gggacttgca
     7741 aactcttatc catctgcctt actgcttgca tttatcacac cattcttatg gttctttggt
     7801 ttacacggag cgaatatggt cgatcctttg atgcaaacaa tcaatgcgcc agccattgaa
     7861 gcaaacgtta atgcaatcgc agctggacat agcgcaccgt ttatcgtgaa caaacctttc
     7921 tttgacagct ttgtcaactt aggaggaaca ggtgcaactt taggtttact gttagctatt
     7981 tatttagtag gacgtaaaaa caaaccatat atggttgtaa caaacttgtc gattgcgcca
     8041 ggtgtcttca atattaatga accaacgatg tttgggttac ctatcgtgtt gaacccgatc
     8101 atgttcattc catttatttt gacaccaatg gtcttagtt
//
LOCUS       DAJSVD010000116         8050 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSVD010000116 DAJSVD010000000
VERSION     DAJSVD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.118"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            119..820
                     /locus_tag="ON498_002407"
     CDS             119..820
                     /locus_tag="ON498_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3940635.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="ON498_002408"
     CDS             complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="ON498_002408"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3940636.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="ON498_002409"
     CDS             complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="ON498_002409"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3940637.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="ON498_002410"
     CDS             complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="ON498_002410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3940638.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="ON498_002411"
     CDS             complement(3439..4161)
                     /gene="tsaB"
                     /locus_tag="ON498_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3940639.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4352..5332)
                     /locus_tag="ON498_002412"
     CDS             complement(4352..5332)
                     /locus_tag="ON498_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3940640.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5356..6840)
                     /gene="galT"
                     /locus_tag="ON498_002413"
     CDS             complement(5356..6840)
                     /gene="galT"
                     /locus_tag="ON498_002413"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR3940641.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6852..7841)
                     /gene="galE"
                     /locus_tag="ON498_002414"
     CDS             complement(6852..7841)
                     /gene="galE"
                     /locus_tag="ON498_002414"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3940642.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2275 a         1716 c         1415 g         2644 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      901 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
      961 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1021 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1081 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1141 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1201 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1261 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1321 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1381 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1441 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1501 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1561 tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg
     1621 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1681 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1741 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1801 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1861 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1921 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     1981 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2041 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2101 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2161 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2221 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2281 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2341 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2401 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2461 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2521 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2581 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2641 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2701 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2761 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2821 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2881 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     2941 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3001 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3061 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3121 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3181 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3241 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3301 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3361 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3421 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3481 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3541 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3601 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3661 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3721 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3781 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3841 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3901 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     3961 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4021 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4081 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4141 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4201 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4261 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4321 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4381 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4441 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4501 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4561 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4621 aaagcgcatc aggaacatgc ggattcgttt ttaaatactg cttcactgct tgatatcctg
     4681 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4741 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4801 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4861 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4921 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     4981 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5041 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5101 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5161 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5221 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5281 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5341 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5401 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5461 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5521 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5581 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5641 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5701 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5761 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5821 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5881 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     5941 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6001 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6061 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6121 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6181 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6241 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6301 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6361 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6421 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6481 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6541 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6601 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6661 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6721 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6781 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6841 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6901 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     6961 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7021 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7081 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7141 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7201 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7261 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7321 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7381 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7441 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7501 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7561 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7621 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7681 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7741 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7801 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7861 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7921 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
     7981 aacattttca aggatttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc
     8041 tcttttgttc
//
LOCUS       DAJSVD010000117         8002 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSVD010000117 DAJSVD010000000
VERSION     DAJSVD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.119"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            18..185
                     /locus_tag="ON498_002415"
     CDS             18..185
                     /locus_tag="ON498_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3940643.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(245..598)
                     /locus_tag="ON498_002416"
     CDS             complement(245..598)
                     /locus_tag="ON498_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940644.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1102..1521
                     /locus_tag="ON498_002417"
     CDS             1102..1521
                     /locus_tag="ON498_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3940645.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1547..1753
                     /locus_tag="ON498_002418"
     CDS             1547..1753
                     /locus_tag="ON498_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940646.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(1981..2154)
                     /locus_tag="ON498_002419"
     CDS             complement(1981..2154)
                     /locus_tag="ON498_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940647.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2130..2795
                     /locus_tag="ON498_002420"
     CDS             2130..2795
                     /locus_tag="ON498_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940648.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(2877..4043)
                     /gene="dnaJ"
                     /locus_tag="ON498_002421"
     CDS             complement(2877..4043)
                     /gene="dnaJ"
                     /locus_tag="ON498_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3940649.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4194..6023)
                     /gene="dnaK"
                     /locus_tag="ON498_002422"
     CDS             complement(4194..6023)
                     /gene="dnaK"
                     /locus_tag="ON498_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3940650.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6074..6637)
                     /gene="grpE"
                     /locus_tag="ON498_002423"
     CDS             complement(6074..6637)
                     /gene="grpE"
                     /locus_tag="ON498_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3940651.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6731..7774)
                     /gene="hrcA"
                     /locus_tag="ON498_002424"
     CDS             complement(6731..7774)
                     /gene="hrcA"
                     /locus_tag="ON498_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3940652.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         2247 a         1656 c         1420 g         2679 t
ORIGIN      
        1 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
       61 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
      121 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
      181 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
      241 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg
      301 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
      361 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
      421 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
      481 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
      541 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
      601 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
      661 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
      721 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
      781 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
      841 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
      901 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
      961 cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc
     1021 cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct
     1081 agacagaaag ggggtgagat tatgaggaca tcttctttcg agcatatcgt cagattacag
     1141 ttcaattctt taatgctgat tgtcattaag aacaaagtga aaagtcgaaa cagacagctt
     1201 gcaagacatt ctaaacgtga agtattgttt tgtgaaatag ccgaaacgaa acaagttgaa
     1261 cgtggaacga ttgatacctg tttttatgat tgtgtatcat ttaaagtctt gcactttaca
     1321 atttatgtat ctgatgaaac acttggtaca gttttacata gactatcaga aaaacagcgt
     1381 agtgcgattt tattacgtta ttttcaagga atgaacgaca gagaaatttc agaactgtac
     1441 catgtatcac gttctgctat ttgcagtaga agaactagag gactaaggaa gttaaaaatg
     1501 cttttgagcg aaaggaagta aagattatgg ctatccctct tttgaattga tttgcaaagc
     1561 gtcaagcgga gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc
     1621 taaattatgc ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga
     1681 tgaagaacta aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat
     1741 cggagtgaag taatcaactg tataaaaaaa tcgggagata cacagtttcg tgttatctcc
     1801 cttagtagtt ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg
     1861 agtgtttttc tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat
     1921 tagatataaa atttaataaa aaccaaaaat gcacggtcgc catgaaggga ccgtgtattt
     1981 ttatcttctt attttctcac ctaatcgtat cagccgcatc gcttgttttc ctgcacaaac
     2041 aagcaatcgc tctacctgtt ccatcgcata agaaagtacc atcggttcat tgatgatatc
     2101 aagtacatag tcgataccac cctcacttaa ttgagcttga tttttcttct ccatattatg
     2161 tagttgtggg acaactttct gacaatcagc aattgtgtca agaacggatc aatcctttat
     2221 tggagttgct cgaaagggtc ttctcttatt acggacaggc tttgctcact gtacatagac
     2281 aacaaatcat tatcctagtc aatcggattt ccagagcctc attgctatca ttgcttgata
     2341 aaatccagac aaagtttaat aaaatgtatc agttacagct aaactttgga attgggtcgc
     2401 tttgttatac agagcaagag acaccgcaat ctttccttca tgccaaacaa gtttgcgaat
     2461 ggatagcttt ccatcaatct gtgaacgaga tccgcttttt tgaagattta gacctcggga
     2521 tcgttttacc agcaatcccg tcagatcaac gaacattata tgtaaaacgt attttgaaaa
     2581 gcctgacaga agaagaagta catttgttca agaagacttt ggcatgcttc agtaaaaata
     2641 acggaagcat caaaaattgc tcagaagaat tatttattca taaaaacacc ttgcaatatc
     2701 gattgaacaa gttccattct ctcactggtt atacccctag aaactatgac gattatcata
     2761 ttctaaagct ggcttttttg ttggtacaaa catgacaact acaaaaaaac caggcgaaca
     2821 tatgattgtt cgtctggttt ttaattatta atgacttagc gtttttacta gaaaaactac
     2881 ttccgctttt tcccgccaaa agcatcttta aacttatcga agaaaccgtc ttcttgctgt
     2941 tcttcaactt tgatgccgcc agcttcagcg aatgcacgta atgcttctct ttgttgttca
     3001 tttagatttt tcggtgtgat cagttttact ttgacatgtt ggtcgcctgt tgcaccacca
     3061 cgtaacttcg gagcgccttt tccacgtaaa cggaagttcg tacctgtctg tgttccagca
     3121 gggatcttca atttcacatc tccatggaca gttggtactt ggacttcatc ccccaaagct
     3181 gcttgtacaa aacttaaagg caattcatag taaatttcag aaccttcacg atcaaagata
     3241 tcactttctt ctacacggaa gaccacatac aaatcaccaa atggtccgcc gttttctcca
     3301 gcttctcctt gaccagctaa acgcatttgt tgtccttctt ctacaccagc tggtacattc
     3361 actttaactg aatgtgcttt cttctcatgt cctgagccat gacaggttgg acaaggttct
     3421 ttgatttctt tccctgtccc atgacaaaca tcacaagttt gacggctcat cactcgtcca
     3481 agtggtgttt ggcgttctac attaatcgta cctgcaccat gacatttatg acaagtagtt
     3541 ggttgtgtgc ctggttttgc accgttccca ccacaagtat gacagatttc ttcccgatta
     3601 tacttgattt ctttttctac accaaaaatc gcttcttcaa aagacaagtc gaccgtatat
     3661 tgcaaatcag caccttgacg aggcgcattt ggatcgactg accgaccgcc tcctccaaag
     3721 aaagagtcga agatatcttc aaaaccaccg aagccgccac cagagaagcc gccgaatcca
     3781 ccaaatccgc caccagctcc accagcaccg taatttggat ctgtgcctgc atgcccgtat
     3841 tggtcatatg cagcgcgttt ttgcgggtca cttaaaatct catatgcttc tgatacttct
     3901 ttgaattttt cttccgcatc aggttcttta ttgatgtcag gatggtattt tttcgaaagc
     3961 ttacgataag ctttctttat ttcatcttct gatgctcctt ttgagagccc taagacttca
     4021 taataatcac gttttgttgc cataggcttt cctcctattg gttctcatca ttttcacgtc
     4081 gcttgttatc atatcatatt ttgtttgaaa caaaaaccga tacttcaaac tatttttcaa
     4141 aagaaaaagc caaagtagca actttggcct tctctcgaaa ttcagtgaac tgattattta
     4201 tcgtcgccat cgacttcttc aaaatcagca tcaacgacat catctgctcc gccttgagct
     4261 gcttctgggt tttcttgagc ttgttgttgt gccgcttgtt cgtatagttt aacagtcaag
     4321 ttttgaacga tttcgttcaa tgcatcgcgt ttagttttca tttcttcgat gttgtttgct
     4381 tcaacagcgg ctttcaattc atcgcgagct gtttctgctt ttttcacttc ttcttcgtcc
     4441 actttgcctt ctaattcttt caatgttttg tccacagaga atagcaatgc atctacatca
     4501 ttacgtaggt caacttcctc tttacgagct ttatctgctt ctgcatttgc ttctgcatct
     4561 tttaccatac gttcgatttc ttcgtctgtt agacctgaag aagatttgat cgtgattttt
     4621 tgttcttttt gtgtacctag atctttcgca gatacattta caatcccgtt tttatcaata
     4681 tcgaatgtta cttcgatttg tggaatacca cgaggagctg ctggaatatc tgtcaattgg
     4741 aatcttccta gagttttgtt gtcagcagcc attgggcgtt caccttgtaa tacatggatg
     4801 tctactgctg gttgattatc tgccgcagtt gagaatactt gtgatttact tgttggaatc
     4861 gttgtattgc ggtcaatcaa tttagtgaac actccaccca ttgtttcgat acctaatgac
     4921 aatggtgtaa cgtcaagtaa aacaacatct ttcacatcac cagtgatcac gccaccttgg
     4981 atagcagcac ccatagcaac tacttcatca gggttaactg atttgtttgg ttcttttcct
     5041 gtttctttac gtactgcttc tacaactgca gggatacgag tagatccacc gactaaaata
     5101 acttcgtcga tttctgattg tgataaacct gcatctttta gcgcttgacg tactggtact
     5161 tttgtacgtt ctactaagtc agctgttaat tcatcaaatt ttgcacgtgt caatgtcatt
     5221 tccaaatgca atggtccaga ttctcctgct gtgataaatg gcaagctgat ttgtgtgctt
     5281 gatacgccag ataaatcttt tttcgctttt tcagcagcgt ctttcaagcg ttgtaaagcc
     5341 attttgtctt gagacaagtc aatgctgttt tcttttttga attctgctac catgtagtcg
     5401 atgattttgt tatcaaagtc gtccccacct agatggttgt cgccagctgt tgacaataca
     5461 tcaaagacac cgtctcctag ttcaaggata gatacgtcaa aagtaccgcc accaaggtcg
     5521 aatactaaga ttttttcgtc gcgatctgtt ttatctaaac cataggcaag agcagctgca
     5581 gttggttcat tgacgatacg ttctacttct agaccagcga tcttaccagc atcttttgtt
     5641 gcttgacgtt gtgcatcatt aaagtaagct ggcacagtga taactgcttt ttcgactttt
     5701 tcacctaaat agtcttctgc gaatcctttg atatattgaa ggatcattgc tgagatttct
     5761 tgtggtgtat atgatttacc ttctacatct actttgtaac ctgcttcacc gatatggcgt
     5821 ttgattgatg aaatcgtatt tgggtttgtc acagcttgac gttttgcaac ttcccctact
     5881 tggatttctc catttttgaa tgatacaaca gaaggtgttg tacggtttcc ttctgggtta
     5941 gcgatgattt ttgcttcgtt tccttccaat actgctactg cagagttagt cgtacctaag
     6001 tcaataccga taattttact cataattaac gtctccttct ttaattgaaa gtgtaagttt
     6061 tatatccgca caattattgt gcaacaatga ccatggttgg tcttaacacg cgatcatgta
     6121 atttgtagcc ttcttgtagt acttctacaa tcgtatcggc tggtgtatct tcagtcgctg
     6181 gaacagtttg tactgcttga tgaagattag gatcaaaggc ttctccttta gcagggattt
     6241 tttcaattcc ttcttcttcc agtgcgttac gcaaactttc caagaccatt tccacgcctt
     6301 ttttcaaacc tgccccttga tcatctgaca cttctgtggc taatgcacgt tccaggttgt
     6361 caattgatgg aagtaatttt ttggctaaat cttgtgaacg gtatttttgt aattgctcac
     6421 gctcattttt tcctcgattt gccatatttg cgatttcagc acgagcccgc aagtatttat
     6481 cttccatttc ttctaactca gcttttaatt tcgctgtttc gaattcttcc gcttcagctt
     6541 ctgaaatacc cgaatcttct gcagcttcga catcgatttc cggttctggc tgagcatctg
     6601 tcatttcttt atctagttct tcttgatttt ctttcacgat acagtcttcc tttcattatt
     6661 cagtcagctg tttgattgct cttactaaga aagagagaac gatcagctgc aaatagcttc
     6721 gttattcaca ttacgagttt gacaagtcaa gcgaacggta gtaatcagca agtttagctg
     6781 ctagttctct tcgataaaca tcgactagac cgaacatccg agaataaggc atactagtag
     6841 gccctaacaa ggctattgta cctcttccat gaccatctat ctcgtaactt gcttgaatca
     6901 tactcatatt ctgcaataaa tcattgccta attcagcacc aatcttgata tgaatattgc
     6961 tatccatcgg agtgatcatt tgtgtaagtc cgtctgagtc tttcataaaa gaaaacatcg
     7021 atttgaactg atcaagatct tgacggggct cataatccaa gagattcatt tgtccactga
     7081 cataaacctt atcttcaaaa acatgaccaa gaacggtgtc aaataactga gacatccctt
     7141 ctgtcgtttg gaaatattta tgaagaatca tcggaatctc cgtacgaagt ttatggtaca
     7201 ccgtcatcag aggatctccg actagacggt cgtttacaat ctgtaccatt ttttctagtt
     7261 cctgacttcc catatattcg ggaagagtga acacttggct ttctacattc cccatatctg
     7321 ttacgacgat agcaataatc tgtcgattat tcaagggtac gatacgaaag ccagtcagtc
     7381 ggcggtcttt tatttcaggt cccaatgaaa aagtcgtata gcttgtcagc tcagataata
     7441 tctcagcaga ttgcttaata atctcattga tttcatgaaa ttctctgcct agagactgac
     7501 gaatcagcag caaatcatct tctgctacgc gagcaggttt taacaaatga tccacgtaat
     7561 aacgatagcc taaagcagat ggaatccgcc cagaagaaga gtgagtcttc aacagcaatc
     7621 cttcatcttc taatgctttc atatcattgc gaatcgttgc tgagctggcc ttgactccct
     7681 cagacatcaa tgttttggaa ccaacgggca cgccactact cgtatagtgt tggatgatca
     7741 gacgtaaaat gtcgttttgt cgctttgtta acatcttcct cacctctcat tagcactcat
     7801 atattctaag tgctaatcca cttataaata tagcaagaag cccttctcct gtcaagagaa
     7861 agactccatt ttttagcact ctaaacacaa gagtgctaat tctaaataat tgatgtaaat
     7921 ccagttatat caacgtttca atagtccttt attgtatttt tgaacttttt ttatttaaat
     7981 tcagataaaa aacaaatatt ct
//
LOCUS       DAJSVD010000118         7792 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSVD010000118 DAJSVD010000000
VERSION     DAJSVD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.120"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            129..1355
                     /locus_tag="ON498_002425"
     CDS             129..1355
                     /locus_tag="ON498_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3940653.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(1513..2130)
                     /locus_tag="ON498_002426"
     CDS             complement(1513..2130)
                     /locus_tag="ON498_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3940654.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(2813..3808)
                     /locus_tag="ON498_002427"
     CDS             complement(2813..3808)
                     /locus_tag="ON498_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3940655.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(3903..4775)
                     /locus_tag="ON498_002428"
     CDS             complement(3903..4775)
                     /locus_tag="ON498_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940656.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(4788..6215)
                     /locus_tag="ON498_002429"
     CDS             complement(4788..6215)
                     /locus_tag="ON498_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3940657.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(6269..7543)
                     /locus_tag="ON498_002430"
     CDS             complement(6269..7543)
                     /locus_tag="ON498_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3940658.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         2580 a         1456 c         1112 g         2644 t
ORIGIN      
        1 tcgctttgat gtagaagata tcaaaggctt tatggatacg ttcaaaaaga tttgaatttt
       61 acttttgact gcatatacta aaagtagcga aatatactgc cttacttttt tattaaggag
      121 tgaggaagtt gcctacgaag ctttcaaatg gaaaatataa aactaatttg agatatccaa
      181 aaaaatttaa agaaattaca ggtatcagtt cagaaaaatt ccaaaaaaca tttccaacta
      241 ggcaattggc tattaaagcc gaaaatgaaa tgaaaaagaa aatcgcaaaa gtcttaagag
      301 aggagaatgc aaattcatta gagctcaaag gtcaaataaa attcaaggag ttttataaat
      361 ctaaatggct tcctagatat gagttagggc aaactactag atcccataaa gctccaagcg
      421 atataaccat cattaataca aagaatttat ttcgattgca tattctgcct atatttggtg
      481 agtatgccat gagctatctt aatgcaaata ccgaaataat atcagatgaa ctcacaaaaa
      541 agtcgaagga atatgcaaac ataaaaatca ttaaatgcta tgttcgttcc atgtttgata
      601 ttgcagaggt tctaaattat attgaattta atcgtacaac aaaaataatt caaagtatta
      661 ctgcacctaa gaaagttgct ctggaagaaa aaagaatacg agaaggaaaa caagctttat
      721 cttcgaaaga actcactgat tggttagacg ctgtaagaga tgatttcaat gatcaattac
      781 tttcctttca cgattacact ctttttatgc ttactttata tcttggggat agaaagagtg
      841 agacctatgc tttgcaatgg aaatacattg attttgaaaa ccaaacagtc cgtcttaaat
      901 atgccttgga taaacacaca aaaaagaaat ttacaaaagg tcgaaaagat actattatcc
      961 aagttccgga aattgtaatg acactattgc aagaatggaa atcagtgcaa gctgaacaac
     1021 tattgaagtt gaaaattaaa cagacacctg accaatatct ttttagttac tccaaacctt
     1081 ctggtgaagt aaattgtcca gtccatactg attacttaaa ttatcgaatt aattctataa
     1141 aaaaaagaca tccccatttg gttcatttaa ctcctcataa acttcgacat acttacgcaa
     1201 ctttagctag acaaggtggg gctgatatga atcaaatttc taacgctttg acacattcag
     1261 gtatttcaac tacaaaaatt tatgtgaata cacctgattt agttgataaa gctgtgttcg
     1321 aagcgtttca gcgaggatta caaaaatcta attaatgtgt tctagaatgt gttctaaata
     1381 taaaaaaaag acactttcca aataattgga aagcgtcttg aacgttgata tataaaggat
     1441 attaacgttt tgagaattga gatgctttac gagctttctt aagacctggt ttcttgcaaa
     1501 attatattag cctcaactga aagatttatt tttttgctcc ctaacttcat tagtaatacc
     1561 atgtttcatt cgatatcttc taaacgttct acgattaatc cctgttagct cttcaacttc
     1621 tttatctgta gatccagtca aaaacaaatt aaatgctaat tgaagtcgcg gatcattctc
     1681 acgatatttt agcgtacctc ccttgtattt cccttttgct ttagcgagtg caattccttg
     1741 ttgttgcgtt tcgcgaatat accgacgttc ttcttcggcc tggtacttgt atagttcgat
     1801 aattaagtta ttaagtaatc gacgtaaatt atcatcagtg atacctgtta acgctggcaa
     1861 attcaaaact tccagtgtag ctttcttcag ctgaattaaa ttcatcgttt ctgttagctc
     1921 tttattattt cgtccaagtc gttttaattc tgtcacaacg acaatatcac cttcacgtat
     1981 gaatttcatc atttcttgaa atccttctcg attggtatct ttcccacttg ctttatcact
     2041 aaaaatttta tcacactctg ataataattc taattgtcgg tctaaatttt gatctaagct
     2101 gcttactcga gcatacccaa ttttcgccat aataaaactc ctttaaaatt ctgaaatccg
     2161 gatcattttt aaattctgtt gcccaactct gttgtcattt ttctagataa atggcataaa
     2221 ttttcagcgc taagtgacaa aagcaactaa tttttcatga tagattttta aatatctcaa
     2281 tctatctcca aaattagttg cttttgtagc tatagttgac tatgaatatg acaaataatt
     2341 agaaaaaact gtgccaatta aatataaaaa tgacactcat ttacattcaa tagaacacca
     2401 agagcccaac agggtatact ctggtgtcct tttaaaaaag taactttctg acctaattaa
     2461 ttgagctact aaaaaagcta tgaaatgagg aataaaaaag ggatcaaaaa cttttaagtt
     2521 tctgaccctt ttgagttcta attatttctt ccagaacgaa gaatgttatc tgctttatct
     2581 gcaattgatt ctgttagaag gactgaaaaa tttatgccag cccttttgcc tagcttatcc
     2641 gcccattgag gaattgttaa ggtttttctg acaagttcta tgtctttcaa atatttggca
     2701 acatctacac agattaaaag catgattttg tatcatagtc tattataaga gatttaacaa
     2761 ttttttatat cacgaattaa taatgaaatt atcattgata gtctttataa atttaattgt
     2821 tctttttctt tactgatgtc ttccccatcc ataatgtgac aggtaaaata tattcctttt
     2881 ttgtttcttc tttatttatt cgtttcatca aaatagcaac agctgtgtct gctatatcat
     2941 taattggttg tattactgtt gtcagatctg gtaatatact ccgagtcatt aaagtaccat
     3001 catatccgac tactaataaa tcagctggta catttaaccc cttttctttt gctacttgta
     3061 atattagggc cgcatcgatg tcatttgaag caaaaatacc atctatttta ggataatcct
     3121 caaacatttg gaaaaaaatc tgttttttct ttacataaga aatattgaaa tctaatgtca
     3181 cagttcgtgg aattaactga tatgctttca tagtatcttc ataggctagt ctccgacgat
     3241 tagccggagt ctgtaagtca attggaccgt ttgtatgaat aatatttttt gcaccttgtg
     3301 caattaataa cttggttgca agttttccac cattgtagtt atcggattca acaactggaa
     3361 tatcttcatt catcactcga tcaattgcaa caatcggtaa attctgatat ttatattcct
     3421 gaattccttg gttatgtgta ccaacaatta aaccatctac ttgatcagac aataactgat
     3481 ttaaataatt tgtttccttt tctttattat tcatcgaatt gcctattaat accttataac
     3541 caatttcata taactttttt tccaatgcct caacgagttc actaaaaaaa ggattggcta
     3601 ctgtaggaaa tagtaacccg acaatgttag ttttcttttg aaataattga cgcgctacaa
     3661 cgttcggttg ataattgagt tcttctatag ccttataaac attatcaatc gttttttgac
     3721 ttaaatagcc acgattgttt aatacacgtg aaacagttgt ttttgataca tttgctcttt
     3781 tggcaacatc ttctaatttt gggcgcattc tcttgtctct cctaaataat gcaataatta
     3841 actatcgaaa aaaagaactc tatagaacta ctatacctga taatcgatat tttttctatg
     3901 ttttattctt gtaattgatt caagcccaaa gcaaagtttc ctagtgttgc cgatccatta
     3961 tttttaatta aaggacgcgt aatatattta cttaacggtg gtatttccac gtaatcatcc
     4021 aataatttat taacattaac acgaattttt tctaacaatt tttcatttac tacgcttcca
     4081 ccaaatacta ttttatccgg tcgtataatc aatgttgttt gaatcacagc ttgtgctaga
     4141 taataggcca ttacatccca aataggatcc gttaaagaaa cgtcttcacc cttttttcca
     4201 aaacgtgctt caaacgttgg tcccgcaact aagccttcta aacaatcacc atgataagga
     4261 caaattcctt taaaatcaat atcatcagga tggcgtttta caaacgtatg gcccatttct
     4321 gaatgaccaa acgatcctaa tagttttccg tcaacaacag ctccaccacc aacgccagta
     4381 ccaacggtgt aataaactaa tgaatttatt ttttcattcg ataaagtgga catgatgtac
     4441 tctccataag ctgatccgtt cacatccgtt gtccaagaca ttggaacatt cagctcatct
     4501 tgtaaataac ctaacacatt aacatttcgc catcccacct ttggtgtaga cgtaatatat
     4561 cctgattttg gtgaattttt tcgaatttct attggaccaa atgatgcaat acctaatgca
     4621 tttatatcaa actgtttaaa atagtcaaca gtttttttca atgtttccct gggggtggtc
     4681 gttggacaaa ttatttggtc ttgtactcgg taatcctcgt ctcccacggc acatacaaat
     4741 ttagtcccac ctgcttctat gctaccaatt aacatcataa aacctcctta ctcgtaagaa
     4801 attgcatctt tttttagatg ataaattgta ctcgagacaa aagcttcttc actaacactc
     4861 aaatgcatac ttggttgatt ttctgtataa taacgctctg taaaaacaag ctcgccatca
     4921 ttaataaaaa tctctgccga acttttatcg ataaacactc gaagtgataa tctatcacac
     4981 atctgaatgg ttccatacct tggatcattt ttgccgttac gagttaaggt acattcattt
     5041 ttatccttag aataaaaaat attgattaac tcttcatctt ttgtttttaa tgaaaatttg
     5101 acttcttgat tttttgattt agtcaatagt acatcaatta gagcttcttt gaatgaagca
     5161 tcttgagcga ttattatttc tcctgccaaa ttaactgtcg tttcaacgcc gctgccaact
     5221 cgaagttgtt ctaattctct cacaggtttc atatacaaac gatctccaat taactctaat
     5281 tctcgaggaa gcgttaatgc tcctgcccat ccgtctactt tttcaggcat agggctttcc
     5341 cacatatcca tccaggccat aacaatacgt ctaccatctg gagataaagt tgtttgtgtt
     5401 gcatagaaat cgtgtccctt atctaattca taaaaatttc ctctaatgaa ttttgctgtc
     5461 tcataatcat aatcaccgat aaagtagcct gtttcatata gatttaaata atcttttcca
     5521 tttgcttcaa ttccctgagg ggataataaa agaatagatt ttccatttaa ttcaaaaaag
     5581 tctgggcatt cccacataaa tccttctgta agtacttcct tcgctttaga aattggtcca
     5641 atgtaatccc aatttaataa atctaacgac tgataaacaa tagcacgacc taatccatct
     5701 ttttcttgac tacccaaaac catgtaatat ttttcatcgt gtttccatac ttttggatct
     5761 ctgaaatgat gtgtgttatc ttctggtgct tttgctatta caggattttt ttcatattta
     5821 gtaaagtgaa tgccatccgt actatacgcc atgttttgat tttgccaaaa atgatctgga
     5881 tcattgtctc cataatagtg atgtcctgta tagaacagat acaacacacc atttttttca
     5941 atagcgcttc ctgaaaaaca accaccttca tcttcttgat ctccaggagt caaagcaagt
     6001 ggtaaagatt cccaatgaac taaatcctta cttctagcat gtgcccagtg cattggtccc
     6061 cattcagcac tatatggatg atgttgataa aacacatgat aatacccatc aaaatagcaa
     6121 aatccatttg gatcattaat ccatcctgat ggtgcagaca catgatatcc caaacgataa
     6181 cgtgtatttg ttaattctat agctcttttt gtcataatat attcgctcac tttctagata
     6241 aaattgtttt ttattctgtt ggtttccatt atcctcgaat aaatggatcc ccttgaacat
     6301 ctctatcatc ttgtttaaga ataaagaatg catatatgcc tgctagtaat acaataagag
     6361 aaataatttt aaatgttggt tgatagccaa cgttgtctct taatattccc aatggtgttg
     6421 ataaaatcac ttgcccaatt tgagcagcta tctgaaatcc tatcatatag agggttgctg
     6481 atagctttgt atcaaaatga agtgtaaaat agcgaaacat tggtaatgta aataatggta
     6541 cttctaaagc atgcaacatt tttatgcatg aaacagataa tggtgtcgag gcaaacccgc
     6601 acaatccaat tcgtacacac ataatggtta cacctaataa caaagtattt cgaaccccta
     6661 attttctcat aatgattgga acaattccca tcattaacgc ttcacaaaat acttgaatag
     6721 cattcaatgt cccatatatt ttttcaccat tagctgatgt tgaaaacaaa ccagtataaa
     6781 atcccggaaa catttgttga tcgaataccg tataaaatgt ccatgtaaaa acaataaaga
     6841 tgattatcgt ccataattga ggcagtttca ttagatcaaa catttctttt aaagaaggaa
     6901 tactatttga ttcagcttgt tcttgattaa aattttgatt tgcttcccgt tcaactttag
     6961 ggttccaaaa taataaattt aacaatagta aagaaccaaa gaaagatccc gcccaaaagt
     7021 ttaaatgagg attttttaca aataaaaatc ctgccaaaag cgctaccaag gcataaccaa
     7081 atgatcccca cgcccttgct tgtccatatt caaagccaaa taaacgacta aacctttctg
     7141 acacagcttc aaaaactcct acagaagcta aatatccagc tgacaaaaat agcgctccca
     7201 taataatgcc taaattaaaa ttgttatcta gcaatggccc atatatccat ataaaaaatg
     7261 gtccaacaag tgtcgctaat acagaagcaa aaattaataa atttcgttta atatacaatt
     7321 tatcttgtaa tgttccataa ataaacatca aaattaaggt aactaacgaa tttgccgaga
     7381 atactgttcc tacagcactg ccagataacc ctaaaccatt tgattcagat gttagccaaa
     7441 gttgaaaaaa tgaccaccaa acaccccaag aagcaaaaaa tagtaataat gtagctgaac
     7501 tttgtaaata agattgattt ttaaatacgt taacttttga catattttta tcctccaacg
     7561 atatatttgt atcccctttc atgataaccg gttaacattt ttagaaaagg acttgtactc
     7621 ctaaaattat aaaagaaatc cgcttacatg tcaaccggtt attctatttg gataatgcca
     7681 tataatgtca caggatatga cttttattgg tgtgtcgctt ttctttttta atggcactat
     7741 tttttataac gccgtttgta taattaagcg agacaaataa aaaagccatt ta
//
LOCUS       DAJSVD010000119         7723 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSVD010000119 DAJSVD010000000
VERSION     DAJSVD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.121"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            41..2368
                     /locus_tag="ON498_002431"
     CDS             41..2368
                     /locus_tag="ON498_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3940659.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2494..2823
                     /locus_tag="ON498_002432"
     CDS             2494..2823
                     /locus_tag="ON498_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3940660.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2863..3867
                     /locus_tag="ON498_002433"
     CDS             2863..3867
                     /locus_tag="ON498_002433"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3940661.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3864..4310
                     /locus_tag="ON498_002434"
     CDS             3864..4310
                     /locus_tag="ON498_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3940662.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4460..5275
                     /locus_tag="ON498_002435"
     CDS             4460..5275
                     /locus_tag="ON498_002435"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3940663.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5481..6653
                     /locus_tag="ON498_002436"
     CDS             5481..6653
                     /locus_tag="ON498_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3940664.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6815..7309)
                     /locus_tag="ON498_002437"
     CDS             complement(6815..7309)
                     /locus_tag="ON498_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940665.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7306..7500)
                     /locus_tag="ON498_002438"
     CDS             complement(7306..7500)
                     /locus_tag="ON498_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940666.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2599 a         1448 c         1537 g         2139 t
ORIGIN      
        1 gtgtaaagga ttatttgatg attttttatt gagaggaggg atgaaaatga ttaagaaaac
       61 tcaaaataaa atccaacgta tatgtcaaaa acattttgtt tctagactca aagctgtatc
      121 taaaagaaaa tacttacatc ccattatgat ttcgatcatc ttgattttta ctttcttagg
      181 gagtttatca agtatttatg taaacgttta tacagaaacg tcaaattttg ctaaccaaac
      241 gacagaggag acaaatagtc caccctattc tgaaatacag tccatagaaa gcaccaatca
      301 gtctagcaac aacgagacaa cctcaaccac atcctcgact caactgattg atccgacaga
      361 aaaagaaatc acagcagcaa aagcagcagg ggagaaagct tattcagaaa caggtagaag
      421 ccagacattt agtactgttg cggccactgc gaatttcaac ggacatacca taccagatgg
      481 atatacagtg atatatgcca aagatggagt cattgttgcg gttaacggat cgatgagtgc
      541 ggataaaact gcgttacaag gcgtaaccaa agcaatcgct tttattgata accgtccaga
      601 tatccataat ctgtttattg gctccacagt aaatggtact actatcacat tcccaaaaag
      661 tatctatatg ggggtggcta ccgctggaaa tacgatcgat tcagttacac ccaatgcgga
      721 taaaagttat tcttttacac cctcaacatg gggagggctt cctgccggag gaggtatgag
      781 tactttttca atcggtatac agttagcaaa tgatattcca ttcacggggt actcgatcaa
      841 aaatgggcag atcgttgatg gtaagggaaa tgtttcaaat tttccaacct atcaagcagc
      901 aacggttcag gtcaatgcga atcggacagg aactttttct tatacagtca atggtggagc
      961 agcaaaaatc ggaacgtacg ggactacatt atctggaatc catgtgggtg acaaagtggt
     1021 tatcactcca acagcagtaa cgggctattc ttttgcacaa acaaacggtt ctttcacagc
     1081 cctagaaagt aacgtcgata cggtgaccta tacaggcaaa caaacaccaa tcactattaa
     1141 gtatcaagac tattttggac agatgattgc tccttctaaa ataatgaatc ttacttacgg
     1201 aagtgctgca caagacctga cgactaatac gcctactatc aacggatata cctttacagc
     1261 tgtttcagcg atagaaacaa agaaccaatc agcaatgagt gttttagcta gtttagatac
     1321 aaatggaaat gtaatcgtaa aggacgcaaa tggaaaacag atttctgaag tcatcctata
     1381 ttataagaca aatgcaacag tatcaatcaa tgcaaatggc agtaagtact atgatggtct
     1441 ttctgttgta ccgatcgtta aatatgcctt taatgataaa acagaatcaa catccctttt
     1501 aaacaatgat ctcagtattg atcaaatcac atggaatcca actgatttca aagcgatgaa
     1561 tgaaaaggga gttgaagtaa ctgcgcctac tgaaattgga acctatacgt cttggcaatt
     1621 gacaaaaagc ggactagcta aactagctgc cagaacaaac tatttattta ctatcgtgca
     1681 gacatctgat gtctatacga ttaagcaaat cagcggtgcg gtagagctca gtggcagtaa
     1741 gacatatgat ggaaaagcag gtgtcccgtc aatccatgtc aagctagcag agggtgtcac
     1801 tagttcgctt actccagtac aagtggcact tagcagtatg gattacacag cagatgcaca
     1861 atcagcaaaa aatatggtta atgctggcag ctatacaatt aaactaacga atagcggaat
     1921 agacaaagtc aaagcagcaa atcccaagtt gtcatttact gatatagcca acacatcagg
     1981 aacttatacc atctataaag ctaacgcagt gattactgtc gatgacgctg cctttaatta
     2041 tgatgcccaa agtcactcga tttcggttgg aaatctccac gttaccggtg ttgtcttagg
     2101 cgaatcattg gattataccc taacagttaa cagccggaca gatattggat cacaaatagt
     2161 aacgattagt ttaggcgaag gaagtgtaaa caataattat acgattatcg caacagatac
     2221 cgctaagttg acgatcaatc cgactctctt gcttccttcg acaggtggga taggtctact
     2281 gccctttgtc tttttaggac tgatctttat tttttctggc ttcttttatt ttatccatcg
     2341 taagaaaaaa gcaggtgaac agcgatgaaa aaaacaaaaa atgtacgtgt ggatatattt
     2401 gctgttctct tttttatcct actggtcttt tttataggtt ctccgactgt gtcagctaga
     2461 gaatcaccta ttacatttac tttgcataat cgaatgttcg aaagcgataa gatgccaagt
     2521 atcagtcaaa acgacggaat aaatatcccg gataactttc aaggagtaaa agggttgaac
     2581 ggcgttactt tcgctatata tgatgtctcg gatgaatttt acaagctgcg gtcagaagga
     2641 agtagtgtgg aagacgcaca aagaaaacta gcacaaaaaa gtgatagtga aaaaatactg
     2701 gcagaaaatg tgacaaaaac agtagacgag gaagagggca ttgcttcttt tagtgcatcg
     2761 gacaaagatg agcaaggtcg tgacgctgtt taccgattta cggaaataaa gacttcagat
     2821 taagtaaaag aaaaatcagc accatttgtc gttgtgcttc ctgtgacaga ttcatctaat
     2881 cataaattga cgaccatcca cttgtatcca aagagcgaac aaaaaatacc tgctgcactc
     2941 acattaacaa aaacagtaga gaataagcag acagattttg cagatgggga taaagtccct
     3001 tatctcatca ccgtaacaat tcctgagaac atcaacgaga taggaaccta tacgatcaaa
     3061 gatacagctg atcctcagtt atggctagaa ccagaaacga tcgatttttt gattggtgga
     3121 gacaagattc atacatttca cacacaaaaa ataaaacatg gttttgtctt atcagattct
     3181 ggaaaagatc ttagttcgtt tgctggaaaa aaattaacga tccgttacca aatgacattg
     3241 aaagaaaaca gtgaaaaaac gacatttgat aacgatgtac gtttgacgac tgacaatcaa
     3301 acagtagaaa cacatcttgc catccaaaca ggaggcaaac aatttgtcaa agtggatcgg
     3361 caaacgaaac aaaaactagc agatgctcaa tttctagtca aaaaaaatga ccaatactta
     3421 gcgcaagaaa acgggataaa tcattgggta gccaaagatg attcaagagc aacgattttt
     3481 acttccagta aagatggaac attttctgtc catggattgt cttttggaag ttacgaacta
     3541 gtggagatta aaccgcctaa aggatatata ttgagtcaat caaccattct ctttgaagtt
     3601 gaaaaaggca gttatgatcc gcttcatcct ataccattgg aaatcatcaa cgaaccaaaa
     3661 gcaaatccga cgaataatgg aaaaacgcca gttcagatgc agacaaatgg tccatcaaat
     3721 gaaaaaaatg gaggaagctt tccaaaaaca agcgaagaga tgcttggagg tttctcaatt
     3781 ttagggttat tgctagtcct ttcaacaggg acagcttggt tttataaaaa acaacaaaaa
     3841 ggaaacaata ggagggaaat aaaatgaaaa acatcaaaac aaaaatatgg acgtttttag
     3901 gaactgctat catgctgctt cctttcgttc tcggcctagg aactgcagag gtttctgcag
     3961 ccgtatcacc tacaccagaa aatgttacgg ttaatttgca taaattgaaa ttcacttcag
     4021 caccagaaaa tcaaataaac aacggaacgg aattgacatt tccgaatagc gaaccgttga
     4081 acggcgttga attcaacgta tatgacatca ctgctacgta ctatccaagt aaagacacag
     4141 cagttccagc agacgctaca cccttcgcaa gtgtaacgac ttctggtgaa ggattagcta
     4201 acctgactct tccaggtaaa tctgatggga aagatgctgt atacgtcttt gtagaaacac
     4261 caaagcccgg tgtcgaaacg tcgccaaata tcgtgttaag cttaccctag accctgacaa
     4321 acctttagac acgattcacc tatatgctaa aaatgtcatc aaagaaagca atatcaaaat
     4381 ccagaaagct gggaactttt caggggtaga taagttagca ggggcgcaat tccagatcaa
     4441 aaattcagag aatcaattcg tgaccggttt tgatgaaaac actggtgtcg ctctctatga
     4501 tcctgctgag tcagcgttga cattcacaac tgatactgaa ggatccatca gtgtcaatgg
     4561 attaatagac ggcaattata ctttagttga aacaaaagca ccagatggtt atcaattaac
     4621 caatccacaa aaagaaacta cttttatagt aaaaaacgga aaattgcaac aaacaaccgg
     4681 tatcatccat ggcgcagatg aagccaatct tgggaatcca aacggtgtag tcaatcggga
     4741 aaatacaact acggataaca cgattactgt agaaaatgtc caaaactacg gtgatttcca
     4801 cttcatcaaa caagatgtga acacgcaaga aaaattagcc gatgcagaat tttctattct
     4861 aagagcaaaa gatccaactg ttaaattaat gaaaaaaata gcaaaccttg cctcaggaga
     4921 atacccatat gcttggtcag atgaagtcac aggttctgac tgggaagaag tgaaattgat
     4981 ttctgattca gaagggaaat tttcaatcga agggctgaaa caaggggcat actgccttca
     5041 agaaacaaaa gcaccagaag gctacgcatt acctaagaat gaagctgcgt acactgaatt
     5101 tacagtagat gatgatccaa caacttctga tgaaacaacg atcgataacc aacctaaagg
     5161 aatcttgcct tcaactggtg gtatggggat catcgtattc atcttggtag gtactgcttt
     5221 agttggagga gcagtgatct atttcaaaaa acgccatcaa gaaacagaag cttaaactat
     5281 tggaaacaaa aatcatacga taaataaacg atagtgattc aaatggggag aaaggttgtg
     5341 acaagtggtc ggtctcttgg tattcgctca acaaacggca aaataggagg aactattttg
     5401 ccgtttgctt cgctaatact caggtcaagg cgctgatttc acaacctctt catatacata
     5461 ttagaaaata ggtggacatg atgaataaaa gcagaataag aatactggac atcttcatgg
     5521 caatcatttt agtcgttggg atcgggatct tttcttatcc ttttgtagaa gattcactga
     5581 atgattttct tgcccagcaa atgatcatcc attatcaaaa gcaagcatcg aataagaatt
     5641 cagaggaaat aaaaaaacag caggaaaaaa tgacaaagaa aaaccaacag ttggcagagc
     5701 aaaacgtatc accagggata ggcagtttca accaagcagt tgatgcaaaa gcattaaaag
     5761 atttacctag taatgccttt tttatggaac atatgcttgg agtcatcgaa atcccaaaaa
     5821 tcaatgtcag cttacctata ttcgatcaga caacggaaat atttttgcaa aaaggaacgt
     5881 cattattgga agaaagttct tatccgactg gcgggaaaaa cacccatgcc gttctttctg
     5941 ggcatcgtgg actcccagaa gcaaaattat ttacagattt gcctaagttg aaaaaaggag
     6001 accagttttt catccagatc aatggaaaga cattggcgta tcaagtagaa aaaatccaag
     6061 tggtcttacc tgatgaagta gattctttgg gtatccaaaa aggcagagat ctcgtgactt
     6121 tgcttacctg tactccttat atggtcaata cacatcgact gcttgtgacg gggcatcgta
     6181 ttccttatca agcaaaagag gcaaaaaaag cgatccaagg aattgatcaa tggaaaaaat
     6241 ggaaattttt tatttggttt atcggtatac tacttggatc aatcggctta gtttggctac
     6301 tcatcgctta tttagattct ttggccatcg ctaagcgaaa ctatccttta tctttttatg
     6361 taaagaacac aaacggacga ccaatcgaag gaatggtctt ttctgtaaaa acgctcaacg
     6421 gcaaacacta tattactaga gaaaaagttc catttgtgaa agcttctgat gaatacggac
     6481 tagtgagatt ttctgattta aaaggcagga actatcgctt gcaacatgag gagctcctgc
     6541 ttaaaatcca tgtaaaacat aaacattcga aacagttttc aatgaaattg aaaaaaggaa
     6601 gatataaact gcgaaaagaa aaagaggtgt actatttgat tgagaaagaa tgaatgaaga
     6661 tgtccaaaaa agcagaggga acagactaaa tgaaaaaaga gagaagctga cgaaatggac
     6721 gtgtaaactg ttatttttgg tttactatct ggaacgccat cttctatttt tttatgtttc
     6781 agaagaattt tattattata gtataacttt tgaattagtt tccataacat ttggagcaag
     6841 gggttaaacc acgagcttga gcttcgctta aagtggcagg ggtatacgta ccatttccac
     6901 atttatgtgt atggtatttt gaaccggtcg gtgtgacata gacagtttgt tcattatttt
     6961 gctcaggagc tgcggcttgt gcctgctgag aggcctgttc agctgctgat tgctgtcgcg
     7021 cctgttctgc ttgttgctgt gcgattgctt cttgcctcgc ctgttctgct tgttgctgtg
     7081 cgattgcttc ttgttgcgct tgttctgctt gttgtcgtgc aatttcttct tgctttttcc
     7141 gttcttcttc tgcttctttt gctttgattg tggaatcaat tgcagcaaga cggcttgtca
     7201 actcttgttt gttttcaggt agcgcttgga tagctgctga agcagttgca tagttttctt
     7261 ttgtaggatt tgcttcagct tgtgacaaag cagattccgc ttctgtcact ttttttctag
     7321 tgcagctttt tccgcttttt gtttctcttc ttttttcttt tcaatttgtt cttgttcttt
     7381 cgatgaatct gccgtacttt cggagatcgt ggtactgctg ctggaagcct gatctgaact
     7441 tgtgctttga gtagtagacg tagtagcacc acacccactc aacaaacttc ccgcgaccaa
     7501 aaacaatgca actaattttt tcgctaaatt tcctccttaa gtaaaaaaca accgttttca
     7561 ataaatgtcc ttttcaattg tttacttact tgttataata attgtgttat gaatagtgat
     7621 tgaggaaact taatctaatc ccgtgttgat acacgggatt ttttgctata gttctgttat
     7681 taagtatgca ataataaaat atgtacgtta ttttattatt aga
//
LOCUS       DAJSVD010000120         7493 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSVD010000120 DAJSVD010000000
VERSION     DAJSVD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.122"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            186..1373
                     /locus_tag="ON498_002439"
     CDS             186..1373
                     /locus_tag="ON498_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3940667.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1465..1830)
                     /locus_tag="ON498_002440"
     CDS             complement(1465..1830)
                     /locus_tag="ON498_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3940668.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1827..2192)
                     /locus_tag="ON498_002441"
     CDS             complement(1827..2192)
                     /locus_tag="ON498_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3940669.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2384..2632)
                     /locus_tag="ON498_002442"
     CDS             complement(2384..2632)
                     /locus_tag="ON498_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940670.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2631..2759
                     /locus_tag="ON498_002443"
     CDS             2631..2759
                     /locus_tag="ON498_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR3940671.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2816..4510
                     /locus_tag="ON498_002444"
     CDS             2816..4510
                     /locus_tag="ON498_002444"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR3940672.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4514..4633
                     /locus_tag="ON498_002445"
                     /pseudo
     CDS             4514..4633
                     /locus_tag="ON498_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4610..4783
                     /locus_tag="ON498_002446"
     CDS             4610..4783
                     /locus_tag="ON498_002446"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940673.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5161..6294
                     /locus_tag="ON498_002447"
     CDS             5161..6294
                     /locus_tag="ON498_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR3940674.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6325..6645
                     /locus_tag="ON498_002448"
     CDS             6325..6645
                     /locus_tag="ON498_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR3940675.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6674..7138)
                     /locus_tag="ON498_002449"
     CDS             complement(6674..7138)
                     /locus_tag="ON498_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940676.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7270..>7493)
                     /locus_tag="ON498_002450"
     CDS             complement(7270..>7493)
                     /locus_tag="ON498_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940677.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2445 a         1453 c         1349 g         2246 t
ORIGIN      
        1 aattctatgt aaataaagaa gtcaatctag aaaagctctc caatctcctg catgtttgca
       61 aaaccacttt actcaatgat atcaataaca taaaaaaaga atttgaagaa cagatcgctt
      121 atacacatcg ggaaaaggac cgttacactt tatatttttc agaacatata cctcgatgca
      181 agatcatgca gcaattgtct caaaattcgt tgtttttaaa aacttgtctg ctttatttgg
      241 aagaagatga acctgattat cttcaattaa ctgaatgtga attcatctct gtcagcaaag
      301 cttattcatt aaaaaaacaa gtattagctt attttaatga ctgcgggatc gaaatcgacc
      361 gctactcccc tcgattcact gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc
      421 gtttaggcgg ttttaattct tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt
      481 tcattgaatc cgtaacggaa aatagtggca gattttatga taaagaaaat aaagaaatct
      541 tatcgattgg ttttgctatc agtttcctac gccagcaagt gtgtgctgtg acaatagatt
      601 caaaatttat cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg
      661 aaaacactga tttccaaacc tattataaaa aagaagaatt cgcctttatt ttgacactat
      721 tcaatctttg taattatggt ttccattctt atcagttgat tgcggaagac tttcaacagc
      781 tacaccaagt gtttattgac aatactcccg aaataaaaga actagttgct acctttgaat
      841 ctcacttcaa tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg attcatctga
      901 tgcgttcggc ttgggataac tatcaattat tcatgccaga aaaattttat cttttaaatg
      961 aggaacagac aaatttatat aaagaagtcc agacaatttt ttccagctgg tccagccagt
     1021 taccttatga tctacggttg aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg
     1081 gaattctccg tctgacaaaa gaacatctga ctatctatat cgtcacaaat tcggatgttc
     1141 attatctgat ttatcgggaa gccttggaag ctgtaactac tttcgatttt caagttgcac
     1201 caacaattta ttcaagtata tctgatataa agaagtatgc gcagcagtcc tcaaaccggg
     1261 tattatgtga acgtacatta tatacaccag atgcggtcca atatgaaaat attattccta
     1321 tttcgatcaa tacgatcgat cgagctatca tttcagctgt gcaaaacaaa taaggggctg
     1381 tgacaaaaat catgtcacag cctcttattt gttgaagatg cccatttctg taacaatctc
     1441 ttgatacacg tcaggctcac aacctcactt attttggaca tctacgggtc cgatctcttt
     1501 atttaaaaaa taagaaatga tggttcggat aactaccaac gccgctagtt ggaaaatatc
     1561 ctgcaaggta ggtttgataa tcgattcgat gatcccagct gaaacaagaa tttccaaact
     1621 tagaagtata tagcctccca acatcttttt aatagcattg ttctcgctga ctcggtcgct
     1681 ttgcactttt gtatggacaa ataaatattt caaaaaatcc ttgattgata gacaaacacc
     1741 ccaaaccaat aaacagatag caaagatatc aagtcccagt acaagttttt cgaaatatgg
     1801 aagaagaaac tgcatcaact ctgtcgtcat cctacctctc ctcttctttg tttttctttg
     1861 tcttctattt cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca
     1921 agcttcaaaa tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact
     1981 agtacttcca agcttaacaa aatataactt gctagcagtt ttcttgtatc tgcattacga
     2041 gtattacgtt ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaatagaaca
     2101 agaacgatcc cgacaattaa gataccgata gaacaaatat ttaaaatagc aatgatccat
     2161 tctagaaatt caattaaatt tgagcgaaac atatacgcta cctactttct tttaatttgt
     2221 tcctattctt ttattgtaaa agaaagttac cttgttcgtc tatttaaaag ttgttctaat
     2281 gataacggtt cgcttaaatt tttctttttc actctcccat ttttctccct ccatccataa
     2341 atcgtggaag tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc gtcatcagct
     2401 tttcaaagaa gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa atagaaccat
     2461 acacctcctc aaatcttttc ttcttaccta ccctattttt atttacagga aaatcagaca
     2521 cttcttcttt tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg cattctaata
     2581 aaaacgaact atccttggat atagaaagag aaaacaagga ggcgtttttc atgagtaaaa
     2641 aattaatcaa agtcggcatc ggtttaggtt tgcttgccct tggcgctgca tatctaggaa
     2701 aaaagacagg actattcgaa gatgacagtc atttatatga tgaattcgaa tcaatctagc
     2761 atcctattta gttgaatcac tacggacata cttggaagac attggaggaa tcacaatgag
     2821 aaaaagtaaa gcaataatga ttggtgccgg tattgccaat atggcagcgg ctgtttattt
     2881 gattcaagaa ggcaaatgga aaggggatca gatcaccttt tattcattgg atgatcacgg
     2941 ttcaaatgat ggtgcaccga ccgacactgt agtggatgaa tattggaata aaaaccatcc
     3001 attagaaaac caaaaaggct atattgctcg aggcggtcgc atgcttaatt accgtaccta
     3061 tgtagatttg atggatctgt taagtcgaat accttctgtt actgagccag ggatgactgc
     3121 tgaagaagat acgagagatt ttgatgcaaa acaccgaacc tttgacaaag cacgtttgat
     3181 ggaaggtggc aaaggaatca ttgatggcgg aaaactggga ttcaataata aagaccgtat
     3241 tcttctgact aaactgattg ctatgcctga ttcagaagaa gaaaaactag acaatatcac
     3301 tatcgaagaa tatttcaaag atgatccaca cttttttgaa acaaacttct ggtttatgtg
     3361 ggaaacaaca tttgctttca gaacacgtag ttctgctcaa gaattgcgtc gctatatgca
     3421 tcaaatgatt tatgaattca ctcaaatcga acatctagtg ggcgtcaatc gaacacgtta
     3481 caatcaatac gaaagtatta tgcttccttt gatcgagtac ttgaaagaac aaggatgcaa
     3541 aatcattttg aaccgccgtg tcactgactg ggaattcaaa gaaacaccga tgcaagatga
     3601 aatcactgtg actggtttga aaattaccaa tacactgact caagaagaag aatacgttgt
     3661 ggtcgatgaa gatacagcag ttattttcac taatgggtcc attacagatt cagctacttt
     3721 aggagattac gaaacaccag ctgtagaaaa tatggattat ggtgcagctt ctagtctctg
     3781 gaaaaaagcc agtgaacgtt tctacaactt gggaaatcct gacaaattct ttgctgatcg
     3841 agatgcaagc gaatgggtca gctttaccct gacgacgaaa aaccatttgt tgttgaatga
     3901 aatcacccgt atcacgactc aagaaccagg aaatgccttg aattcattta tttcaaccac
     3961 accgatcacg ccgttaggcc aaaaagatgt caatatgtct attgtcgtgc atcatcaacc
     4021 gcattttacg tctcagaaac caaatgaaac agttatttgg ggttatttcc tttatccaag
     4081 acgacgtggc gaatttgtgg ataaacagta tattaaaatg aatggaaaag aaatgctaga
     4141 agaactgatt ggacaacttt caaaagtaga tcctggacca gtgaatattc gtgaaaaaga
     4201 aacagaaatt ttcgacagcg taataaataa tatccctgtc tatatgcctt atgcttccgc
     4261 gttgttcaat aatcgtgcca aaagcgatcg accaaaagtc attcctaaac attcgaccaa
     4321 tctggccttt acaggagaat ttgtcgagca gccttatcag atgatcttta ctgaacaaag
     4381 tgctgtacgt tctggagaaa tcgctgcttt ccattttgcg ggtattccta tgtcccgtct
     4441 tgtcaaaaca ccgcgttacg acaaagatat tcctactcta gctcgtgccg caaaaaaaat
     4501 gtttgaataa tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccag
     4561 gctaaaacag cttcttatgt ttttgatttg gttggactta ttaccgcaga tgcaagcttt
     4621 tgaacaccgt ttaccaagag gttatgaaaa agctgccctg catcaagtaa taagagcagc
     4681 caagctaaaa cagcttctta tgtttttgac ttggttagac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt tagtttcgag tatgaagaaa
     4801 aattttgtga aaatggttcc tcacgttttt gcataaattt gacttaatac cgagaaactg
     4861 atcccttctg tcataacctc tttttattta tgtctgtttc tttttgaaaa caactatttt
     4921 ttcaacgttt actctcttgc caatccaaca tcatctaacg gatacaacta tttcaatgat
     4981 taccattaca ctaaaaaatc ggaaacacac ctctccgaac acaaatacat aagactatag
     5041 aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct cctttaaaat
     5101 gaaattatct gtagtactaa taatgcagac aattttatga atctgaaagg agcatctcca
     5161 atgaaagcag tcacttggca aggaaaacag aaaatggaaa tcagaaacgt agatgatcca
     5221 aaaatattag aaccaacaga tgcaatcatc cgtatcacag caacagccat ttgcggctcc
     5281 gatctgcatc tttaccatca tggtgaagca gttttggaaa aagggtatgt tgtaggacac
     5341 gaaccaatgg gaatcgttga agaagtggga tctcaagttc aaacattgaa aaaagggcaa
     5401 cgtgtggtta tcccttttaa tattagttgt ggtcattgtc atttctgcct gaatcacatg
     5461 gaaagccaat gtgatgaatc aaatccagat cagttatatg gcgggctttt cggatttggc
     5521 aagttgaacg gtaatcattg gggtggtcaa gcggaatatc taagagttcc ttatgcagac
     5581 tctacttcat ttgttgtacc agataatgat cttcctgatg aaaaagtttt atttctatca
     5641 gatattttgc ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa aggcgataca
     5701 gttgtgattc taggttctgg tccagtcggt ctattcgctc aaaaattcgc tgttatggct
     5761 ggagcagagc gcgtaatcgc agtagatcca gtacagcacc gtctagataa agcggctaat
     5821 tacaatgggg ttgaaactta tttcttagaa gacacggcac aagctggaac agaattgtat
     5881 gaactggcta aaggtggcgc agatgtcatt atcgattgtg taggaatgga cggattagag
     5941 cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa gcggcacaat ctctccatta
     6001 cagatggcat cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg tgtttatatg
     6061 actccagctt cttcttaccc gctgcaagaa ttttttatgc gggatatcgc tgtcaagcat
     6121 ggacaggcac caattatcca tctaatgcca aaaatttacg caatgattgc agaaggacta
     6181 ttcgatccta cacaaatcat tactcattcg ctgccactat cagaagcagc acaagcatat
     6241 gagatctttg acaaaaaaga agataaaaat ataaaagttg ttttgaaacc ttgaattcag
     6301 taaaaaatac aggtgatttt ctttatgccc aaaatcaaac gaaataccca tgagatcgaa
     6361 ataggaaaag aagaagatat cgaaatacga ggacgtcgct tccgcttcta tttccaaaat
     6421 cgttacacat tgatctcact ggctgtcgat ttattaaccg ggattttcta tatttttgga
     6481 agccttgctt ccttaactaa aatccccgat cgatatggca cctattttta tttggctggt
     6541 gctattttct tgacgattcg tcctattctc agaatcgtaa ggaacatttt tatttacgac
     6601 gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg catgagaatt tttcaaatct
     6661 catgcctctt ttttcaccct atcgtatcac tttgatacag acgagcggtc gtcccacttt
     6721 cttccttgac cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg gtatgatctg
     6781 taattaagta aagtttttcg tacccaagtt gatgcatttc ttttctgatt tccgctaata
     6841 tccgcttggc tatcccctgc ttacgctttt ccggttcaac aaacagggca caaagatttg
     6901 gtgtcagatc agggcggtta tggaagtcgt tttcaatgac gcccgctcct ccgacgatgg
     6961 tttctttttc atctaagacc acataccatt gaggaatact ttccttatta gcgattccag
     7021 tacgaatact ttcctcatac acaacagcag gaactcccca ttttgatgaa aaccaattcg
     7081 cgctttgttt tactaattct gggtgttctc ttaatttgat ccagttatat gtaaccaatg
     7141 tttaaacctc cgactaaaat aaaacgttta caaaaattat acaagtcttt atgaaaatta
     7201 gcaatgacaa caatagtgat gtaaaaaaaa aaacggaaaa caatcacttg ttctccgttt
     7261 tttgattaat caagcatcta aagcttcttt tcttctttct tggatcgtag ctaccttgac
     7321 gaacggtaga tagatcaaaa tagaaagaac caaattgaca gcggctagta caccacctgc
     7381 aatacttttt gttgccaata caccgccaat aattggtgga gttacccatg gaggcataac
     7441 ggtagctgct ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa cta
//
LOCUS       DAJSVD010000121         7397 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSVD010000121 DAJSVD010000000
VERSION     DAJSVD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.123"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..142)
                     /locus_tag="ON498_002451"
     CDS             complement(<1..142)
                     /locus_tag="ON498_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940678.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="ON498_002452"
     CDS             complement(183..437)
                     /locus_tag="ON498_002452"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3940679.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="ON498_002453"
     CDS             complement(548..802)
                     /locus_tag="ON498_002453"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940680.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="ON498_002454"
     CDS             complement(995..1270)
                     /locus_tag="ON498_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR3940681.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="ON498_002455"
     CDS             complement(1242..2195)
                     /locus_tag="ON498_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3940682.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="ON498_002456"
     CDS             2807..4300
                     /locus_tag="ON498_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3940683.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="ON498_002457"
     CDS             4435..4731
                     /locus_tag="ON498_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3940684.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="ON498_002458"
     CDS             complement(4755..5714)
                     /locus_tag="ON498_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3940685.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="ON498_002459"
     CDS             5897..6748
                     /locus_tag="ON498_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940686.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..7052
                     /locus_tag="ON498_002460"
                     /pseudo
     CDS             6769..7052
                     /locus_tag="ON498_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DAJSVD010000122         7361 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSVD010000122 DAJSVD010000000
VERSION     DAJSVD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.124"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(718..3867)
                     /locus_tag="ON498_002461"
     CDS             complement(718..3867)
                     /locus_tag="ON498_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3940687.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3881..5125)
                     /locus_tag="ON498_002462"
     CDS             complement(3881..5125)
                     /locus_tag="ON498_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3940688.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            complement(5118..6710)
                     /locus_tag="ON498_002463"
     CDS             complement(5118..6710)
                     /locus_tag="ON498_002463"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3940689.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT         2096 a         1497 c         1170 g         2598 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gcgtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccctcc ctaaacaaac atcttttgta
     3901 agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtgt
     3961 cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc ctagtaaaag
     4021 gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt ccttcagtgt
     4081 tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg aactcattat
     4141 ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc gtcataccaa
     4201 gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg aattcatttg
     4261 gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca tcaaactgtt
     4321 ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag tctaaatctt
     4381 tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt tcccaatctt
     4441 ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg aacatttttt
     4501 gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga agagcgatag
     4561 tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat attggaaatt
     4621 gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct gaacctggat
     4681 tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata aaatattctg
     4741 aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat acacccggtt
     4801 ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc atgataaaat
     4861 cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta attttatatc
     4921 ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa ttgctattag
     4981 atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact ccatcttttt
     5041 tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct gggaaacgaa
     5101 tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg taaccacgct
     5161 tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt ttctagcact
     5221 tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc aattggttct
     5281 tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt ttctttgatt
     5341 tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc ataggtttcg
     5401 aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt gacaaattct
     5461 cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa tacaatcacc
     5521 gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac agcataaata
     5581 ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg gaataaaact
     5641 ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa accatgaaga
     5701 agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt aaaccgagaa
     5761 tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt cattactact
     5821 gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc cccattgcgg
     5881 agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc tagattgtat
     5941 gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt taagtagtta
     6001 cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact aaagaatcgt
     6061 cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat atgaggcgtg
     6121 taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag aaattcataa
     6181 gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa tttcttaatg
     6241 acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt tgattggaga
     6301 tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg cacaaaggct
     6361 ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa gacgttgaaa
     6421 agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat cagatcttct
     6481 tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct taccgtatca
     6541 tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg gtcggataaa
     6601 tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc cattttgcta
     6661 cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa aaagatacac
     6721 tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc ccttttagaa
     6781 aaagcaaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga aaggagtcta
     6841 acgttgtttg atataactta gactgatcgt taacttattt tttatatttc tacatagtta
     6901 tctttagaac catttcagat acatatgaaa caattacata tgattttttc ataaaaaaac
     6961 agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac taaaatcact
     7021 acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct ccgtttcatt
     7081 tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc cagtagctta
     7141 tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg gtaaataaat
     7201 gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg taatcagtta
     7261 cctgtccgtt cttaaatacc acagaataat cttttttccc attttcaagt aaccgaagtg
     7321 gaatatcttt accaactttc tctgtcactg cattactgat a
//
LOCUS       DAJSVD010000123         7325 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSVD010000123 DAJSVD010000000
VERSION     DAJSVD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.125"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(57..350)
                     /locus_tag="ON498_002464"
     CDS             complement(57..350)
                     /locus_tag="ON498_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR3940690.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="ON498_002465"
     CDS             complement(378..1043)
                     /locus_tag="ON498_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR3940691.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="ON498_002466"
     CDS             complement(1183..1815)
                     /locus_tag="ON498_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3940692.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="ON498_002467"
     CDS             complement(1822..2889)
                     /locus_tag="ON498_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3940693.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="ON498_002468"
     CDS             complement(2886..3617)
                     /locus_tag="ON498_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR3940694.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="ON498_002469"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="ON498_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3940695.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="ON498_002470"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="ON498_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR3940696.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="ON498_002471"
     CDS             5780..6949
                     /locus_tag="ON498_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3940697.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         2222 a         1481 c         1125 g         2497 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacc
//
LOCUS       DAJSVD010000124         7104 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSVD010000124 DAJSVD010000000
VERSION     DAJSVD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.127"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..328)
                     /locus_tag="ON498_002472"
     CDS             complement(<1..328)
                     /locus_tag="ON498_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940698.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELAD"
     gene            complement(536..1462)
                     /gene="glsA"
                     /locus_tag="ON498_002473"
     CDS             complement(536..1462)
                     /gene="glsA"
                     /locus_tag="ON498_002473"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3940699.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1485..2696)
                     /locus_tag="ON498_002474"
                     /pseudo
     CDS             complement(1485..2696)
                     /locus_tag="ON498_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2731..4344)
                     /locus_tag="ON498_002475"
     CDS             complement(2731..4344)
                     /locus_tag="ON498_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3940700.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4456..4665)
                     /locus_tag="ON498_002476"
     CDS             complement(4456..4665)
                     /locus_tag="ON498_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HCR3940701.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(5654..6868)
                     /locus_tag="ON498_002477"
     CDS             complement(5654..6868)
                     /locus_tag="ON498_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3940702.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(6996..>7104)
                     /locus_tag="ON498_002478"
     CDS             complement(6996..>7104)
                     /locus_tag="ON498_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3940703.1"
                     /translation="YIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT         2011 a         1525 c         1169 g         2399 t
ORIGIN      
        1 gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa aactttaaaa
       61 aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca tgtttgaata
      121 cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct agacactcgg
      181 ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa atagctgttt
      241 gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact tttctaaata
      301 tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt ttcttatgat
      361 tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga aaagcccttt
      421 caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa tttcagcctc
      481 attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag aatggttatt
      541 caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata ccagctgcgc
      601 tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga tttggcacag
      661 ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg acggaaaact
      721 ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt gtagcacttt
      781 cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc agcacagcag
      841 ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa taagcatcga
      901 gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa gcaagagagc
      961 gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat gtgatattag
     1021 gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca tctgcatctt
     1081 taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga ttcattggat
     1141 gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg gttggttccg
     1201 tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat aacaatacgg
     1261 gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc tcaatcagtt
     1321 gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact tcagctaatg
     1381 ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt ccaatcaatt
     1441 gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt ttctgcatgc
     1501 tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat aagataagaa
     1561 acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc aggtggaaca
     1621 aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac gattactttt
     1681 acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt aaaaaccagc
     1741 ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac agtcaatgac
     1801 atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac ggctgcccag
     1861 attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg tgtgttttct
     1921 tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc ttttgaagga
     1981 gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat cgcaagaatt
     2041 ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa cgattgaatc
     2101 acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt caatccgcca
     2161 aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg ataatccttt
     2221 ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat atatgctaaa
     2281 ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat caatgttttc
     2341 atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc taagccaaac
     2401 aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat caatgaagtg
     2461 tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa taggattgcc
     2521 aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga taatgcacca
     2581 gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg gaaaaagatt
     2641 gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac accgccggtt
     2701 tcccttagtt aaaaatatta ctcttgttga ttactcaact cttccaataa atcggtaata
     2761 tatctcaatt tcctgtattc tttcgttatc ctcattcgtt gttgcttcat gtatgaggat
     2821 tttttctatc atggcattta ataatggtgc tgtcagttcc tttggtacgg aatattcctt
     2881 gattaactca atccactttt cagcacctat catatcctgt tccattttac ttagttcttc
     2941 tcttaggctt gttatctgct gttccagttc tatctgttct ttttggtact tgctggacaa
     3001 catcacaaaa tttcgctctg ttatcttctc acaggctcta tcttcataca ttttcgcaaa
     3061 taaacggtca atctcatttt gtctgttctc ggctttcttc aatgtacttg cctttttctt
     3121 cttttcccgt attctctctg cattgccagc tttctgtatc ttgttcaata ccttttcttc
     3181 gtcctgctgt actgccttag cccaatactg caagcgttcc aatacggctt gatacagcac
     3241 atcataacga atgtagtgca tagaacaatt acctttgcca aactgcccgt agtaactgca
     3301 agcataataa ctgtaaggcg ttttatttgt cttattcgtt ccaaatctca tagaccaacc
     3361 acaatccgca cacttgacaa gccctgcaaa aatcggcgtt gccttttcct ttgtctgtct
     3421 gcgtcttgat tttatctgct cctgcacacg atagaacact tccttgtcaa taatcggctc
     3481 gtgagtgttt tctactcgaa accattcact ttcgggctta cgcactttct ttttgctctt
     3541 gaacgatacc gtagactgca tattgtgaac actgtttccg atataaacct cacttttcaa
     3601 tatcgccttg acatgagcaa tcgtccactc ataacgctta ctttcgggtt ttccctcaaa
     3661 gatatgtgca aaagtaccgt atcttgtaaa atttagccat gacgctgtcg gaactttttc
     3721 tgctcttaac tgctttgtga tttttgcact tccgtaacct tgataggcta gggaaaagat
     3781 tttttcaata atccattttg tttcatcatc aaccaacagt tttcctttga tgtcagggtg
     3841 tttcttatat cctaacggag cataagcacc gtaatgagcc ccctccgcaa actttgtctt
     3901 aaatgctgat ttgactttac ggcttgtatc tctcgccacc cattcattga tgatgttctt
     3961 aaatggtgca atctcacttt cgcctttttc gctgtctaca ccgtcatcaa tcgctatgta
     4021 gcggacatta tgactaggga aatacaattc tgtatattgt cctgtcataa tataatttct
     4081 gccaagacgg gaaaggtctt tcgttacaac acagttgatt tttcctgcct ctatatcctc
     4141 aatcattctt tgaaaactcg gtctgtcaaa atttgttccc gaccagccgt catcaatata
     4201 ttcatcaatg acattcaaat gatgttcttt tgcatataaa cgcaacatac ttctctgtgt
     4261 ggtaatgctg gaactttcgc cctgtaattc atcatctcgg cttaacctaa gataaagtgc
     4321 agtattgtaa atctgttgtt tcattgtcag ttatcctcct ttataactaa cccgtgttta
     4381 caatacctgc ttgcaagtaa agtataacac gggtactttt tgtcattcaa tctatttctt
     4441 acaactgcac cgattttatg cgattgtttg ctttgctttt tccagcatga catcagttag
     4501 aagttcttcc attgttttgc ccttttcgga aaaatgctct tttactacaa tctttgtctt
     4561 tcctctgttg ccaatgccac acttgccatt tttcttttga attgttttta cagcattatc
     4621 aataacacat accccctttc tgtaaaatct ttctaagcaa ttcaacggct tttgtcatag
     4681 cccacaggag ttccacctct gcatagttct tatgtagccg tacccattgc gtgcgacgct
     4741 cttaatgctc aagctgtggc tgtacgggag tatcattatc tgacagtaca ggtcatggcg
     4801 ataaaagagg acaaatcttt tacaagaaca caaataggtt ttcgctcgct cttgcagggg
     4861 aaaggtcgtg gcgtatttgt ttcaacggct agctgtctgt caaacgtatt gaatagcttt
     4921 attcagttgt caaagaacga ttgaaagaaa aaaatcgtct ttcctatata tcgcgttgga
     4981 aaagaggaga aacgtaacca aaatccaaaa ctttttccat tattagtaca aatggaacgg
     5041 ttcattttgg caatcaacgg ttgattttct cctttttcac tactatctgt acagcaaacg
     5101 tccatttgct aagtttaaat ggaaaaattt taacttcttt tcatgcacca tatagggata
     5161 gaaaatgtca ttttgctaag ttgatgatga aaaaagaata ttttcctttt cacaccctaa
     5221 ctacccaaac cataccgtta ctgccaacaa tagaatgaaa tattttcttt gcaccatata
     5281 gggaacggaa accttaaatt gttaagttga gaacaaagta ttttcatctt ttcacatcaa
     5341 cactcaactg ctaatgcctg tttgaataca aaatgatgat gtttttcctc ttttcacaga
     5401 agactgtcca ctaatcacga atttccgtca ttttttcaaa acttttttct tctttcactt
     5461 catataggga cagcaagccc cgatttaatg acctattttc aaaaaaagtt tagatttcct
     5521 tttctcgcac catatctgta cgcaagtagc cattttgttg cagatttaat aaaaaattta
     5581 tttttcttct ttcacttcac atagggaatg aaaacagctc tttgctgact aaatctgaaa
     5641 tttttcaatt acattaaaaa ataccgtcat tctcttgtgg aacagcggta tctatacgtt
     5701 cttctatggt tgatttctct aattgtaata aatgtttatg cttgtctttt agttctgcct
     5761 gctcaatcat atctttcagt atcgttgtac gattttctct gtctaatgcc tgtaccattt
     5821 ttatggttgg tgcgacttgt ctttgtagcc aatgcaacgc tttttgtaaa gtgtaaggct
     5881 ctggctttgt ggttaagcgt atcggctctc tgttttcccc tacaaaccat gcccaatcat
     5941 catttgttat ccattgactt tttggcttgt cgtcctctct gtcaacaaag cggacataat
     6001 gattgatgat agaaaaagcg gtgcgttcag cgtcccgata ggtcagtaaa tctacaaccg
     6061 cataataggc tcgttcgttc ttcatgcgta tttcaaaacg gttcttaatc tccgtatctt
     6121 caatttctgt gccattcttg acatactgct cataattttt ctgataagca cacatatata
     6181 attcgctact tgttgaaccg agatataagg tttctcctgt attctttggt aaatcgctac
     6241 cgcatttttc tgtaccgctg taatctttct gcatacggaa gtaggacata cattcgccag
     6301 ccttgtattt ttcctttaac tttggaatat ccaatattcc tgctttatca ttgatagcca
     6361 aatcaagccg tttcatcaca ccaccagccg tcatacaatc cagcatgaag tcataccatg
     6421 agcgttcctg tgccagcaga taacattcca tttgccgaca gcctttgcct ttcagttcca
     6481 ataaaacacc taaatgctcc tgcatggaac acatgatatt gatgtcgcca agtacatatt
     6541 tgctttcata tccgtatttt ccataatctt cataaagcat gtaattagat ttcagttgta
     6601 atacatcacg gataatcgcc aatgcgtccg tagtaggaaa gcggacacga aagtaatcaa
     6661 tcaataagaa tagtggttct tgcggattga aacgctcaat ctgtttttct atgatttctc
     6721 ttaattcgtc attcaatggc tgttttcctt tttcaatgcg gttgtaatat tcacggctga
     6781 taccacaggc aacagcaagc cttgtttgtg ttactccgta ttcctcacgc ttttgtctta
     6841 gttcttcaat aaagttttta tcattcattc ttcaattcct ccaatcaaaa aaggcaacta
     6901 ccataattga taattgcctg tcattaaaaa tctgtataga aaaagcagtc aatcctaaga
     6961 ctaactgctt tgtgtgtatg gatataaaat tgcttctaat cttgaatagg tatccaaatt
     7021 tcaataactg aattatcact attccaatga aaacgataat cgtatttttc aaagtgtagg
     7081 ataatatctt ttataggaat atac
//
LOCUS       DAJSVD010000125         7089 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSVD010000125 DAJSVD010000000
VERSION     DAJSVD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.128"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..3125)
                     /locus_tag="ON498_002479"
     CDS             complement(<1..3125)
                     /locus_tag="ON498_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR3940704.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDEL"
     gene            complement(3139..4356)
                     /locus_tag="ON498_002480"
     CDS             complement(3139..4356)
                     /locus_tag="ON498_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR3940705.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4346..5941)
                     /locus_tag="ON498_002481"
     CDS             complement(4346..5941)
                     /locus_tag="ON498_002481"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR3940706.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6066..6851
                     /locus_tag="ON498_002482"
     CDS             6066..6851
                     /locus_tag="ON498_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HCR3940707.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2065 a         1439 c         1177 g         2408 t
ORIGIN      
        1 atcaactcat catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat
       61 ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
      121 ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc atgtaattct
      181 ttctcagaaa caaaccactc attggaaaag gcatgaatca aattatcact tgcttgtgta
      241 aaatagcgac gtttaatgac aaagatatct tcattactat ctaaagtccc cacatcaaat
      301 gcatttaaaa tttgtcggta aacttctttg actccttctg ttttattcaa tttagccaaa
      361 gctttttcaa tttcttctcg tgcatctgaa ctattcgcag cataagttcc tagtaattga
      421 tcaatataag tagaatcaat atcatacaac ttagtggtat tttcactata aaattcgatc
      481 gttgaaaaat cttctccctc tttttctggt tcttcctcca tcaatgaacc cttcacggtc
      541 tcgtacacgc caatttgttc ttccaaaagt tgaatttgat tgttcaaagt tgaagattgc
      601 accatgtcat cattgtaatc atcataagtc actaacgatt catacgcacg attcaattct
      661 tgaaatgctt tgacgtactc gatacggatt tcaagtggcg tatgttcatc aatggattcc
      721 atggatgaaa cctcttttaa cttaccatat gcttgcttga atcttttttt ggattcttgg
      781 taacttggat aaatcaaacc cgattcttct ttagcttctg aatacagttt tgtcgcatcc
      841 tcgacattct tctccattgt cgctggcttt cggaaagtaa caattagtcc ttttgttttt
      901 tcaggatacg tacggttcgt acgtgaaaag gcttggatca atcctgcata ctctagatta
      961 cgatccacaa atagggtttg aatcgtaggg gcgtcaaaac ctgtcaatag acgatccact
     1021 acgattacta aatcgatttg tttcccaaat tgtttgaatt ccgctctttt tcgtgccaaa
     1081 cgattattga tgtctccgtt ataacgctca atgtctgcta gtgaccaggc agtatcataa
     1141 tactgattat attcctcaat aattttttgc atttcttctt gttgggcact tgaatctcgg
     1201 ctattttctt ctaatgaata ggtaatagcg attcgtggga aatcaggatc ctccatcgtt
     1261 cgtccttcac ggataggctg attgggaaac tcttttatta accaatcaga ttctttcgtc
     1321 atctctttga tggcccgata atactttttg gccatatcaa tggaactcgt ggttaagatc
     1381 gcagacttcg ttggacggcc atttcgaaaa tcaaattttg tataggcatt gtctggacgg
     1441 aacattttat gaatgacttt ttggatatgt tcctctgctt caaaaacagt agggtccaaa
     1501 taactttctt ttttgacaga ctccatttga tcgatcatac gattgatctg ttccggtgaa
     1561 tactctgcat atgtttctac ttcttttaat ttccgataga ttttattctc taatgaagtc
     1621 ggctccacgg tgttttcatg ctctacttga aatcctaaaa ccgagccatc ttctaatgca
     1681 ttcttgatgg tataggtgtg caagacctct ccatactgat catgggtcgt acgtgccaat
     1741 tgccctttgg cttgtttccg attctcaggg aaaatcggtg tacctgtaaa gccaaaccaa
     1801 gtggatttcg gaaacatttt tttgatttct tccatatttt cagcacttaa cgctcgatga
     1861 cactcatcaa ctacaaacac gatatgttgc cccattaatt tttggaagcg attggttcct
     1921 tttttctctt cctgtttctt tgcatatttc aacgccgcat ctagtttttg acgggttgta
     1981 atgatcacaa cattcgcatt ggcatctgcc aacagagttt cacttaactc tttggcactt
     2041 cctgttccca cgatcaagct attcgctttg gcattaccag aagaaacccc agtattaaac
     2101 tccgaagcaa atttcgtaaa ctctgtggtg gtctgattat ctaaatcttt gcggtccact
     2161 aacatgatcg ttcgatcgat ccccgatttt cgtgctaaaa gcttagtaga tacaaaacta
     2221 gtcaacgtct ttcctgaacc agtcgcatgc cacacatacc ctgattgatg tttatttgcg
     2281 gcaataaaca aggcttgaat cgcatgcact tgataaggat gtaacaccat gagggtttta
     2341 ttgtcttgat cttcactgac aatcgtataa tccgcaatca agcgatgggc atctgggata
     2401 tttagcactt gtttgcaaaa ctcatataag ttttctacct ttttattatc tttcgttcgc
     2461 caactaaata aaaacttagg atgcaaatcc ttcggtaagg catttgcaaa ataccgagtc
     2521 gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa agatattgtt ccgaaacatg
     2581 ccttcttcag catatttttt gatttgatta aacgcctgat aaaaaccatc ttttgcagtt
     2641 acttgtttta gctcgatttg gataatcggt aagccattga tcaataaggt gacgtcaaaa
     2701 cgtcgatccc gcgcttctat gttacttcca cgttttgcaa tttgatggac cacttcataa
     2761 gtagagattc ctccaccaat gtcttgattg gaataaagga taagtgagac agaacccaat
     2821 tgcggatttt cccgttcaat cgtgatccga gccatcccat tttcaccttt taaccatttc
     2881 gcagcatcaa atggggtttt cgtccgtagc aataattcgg ttttgatcgt ttcaaactct
     2941 ttatcggtca aaggagaatc attcagttct gcttgattat ttgagatgat tttttgacgt
     3001 aaattgctcc aaagatcttc ttctgatttt agatccggac gatacgtcca ctgattatgt
     3061 ccttccccga ggacacgaat caactgttct tcgacttccg cttcctcacg gtgactaatt
     3121 ttcattcttc ttccctccct aaacaaacat cttttgcaag aagccttttt tcgtttcctt
     3181 cagcaaatct aacttacgtt gatgaagagc gatcgtatca tctagctgct tgaagaaggc
     3241 accaattttt tgttgttcat ttatatccgg tggaaactga aatgtccttt gttctatacc
     3301 acctttagtc acatgtacca ttgtcgttcc gttagttgat tctttaattt tctctttatc
     3361 agtcaatagc caagtatata gatactgttt gtccaattcc ttagataaaa atttaagttt
     3421 ccaaatatga taatggtaga ttacacgttc ttcattccac atttcaggac cgaagcttgt
     3481 tgcccataaa tataagagat ctcctttatt agcatacttg tcttcctcta attctaagtt
     3541 tgaataatac catctatcat tagtattaaa attacctact cttaaaaccc tatattttcc
     3601 agaatcaagt aattcttgtt gtttaaaagc tctaccattc aaaaaaacta ccacttcccc
     3661 caacttccgc tcttcccaat ctcccgtaaa tcctggaaaa cgaacttctg gcacttttgc
     3721 tccgttttta gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa
     3781 ttcgtgctgg tgaagcatga tggtgtgatc aagttgtttg aaaaatgaac cgatcttgat
     3841 ctgttcgtca taagttggaa gtgagatagg tgtcttttta ccactatcaa ttgtataggt
     3901 tccgacggtt ccaatgcctg caacgtcacg gatatacttc tgaattttgt tatttatgaa
     3961 cgagtagtaa aagaagtttc cgtcaatttt atgctcattc ttgaaccaaa tgatgtttcc
     4021 atccttaaag taaattgggt tatcatcagt gattaataag gggacaccta ttgatccgac
     4081 accagttaca agcaagtctc cttgagaaac tttacctgaa tttttcgaat attcattata
     4141 tttttcttct gaaatgtaaa gataatcgga tggttcctca ttttttgcag ctgagacaat
     4201 atcacgagca cgtaaaaatc taatacctga gttagtccaa tctgattgat gaattcgctt
     4261 cacagaagta acatccatca tatctcctaa cttacgctct tcccaatctt ccgtaaaacc
     4321 aggaaaccgg atttctggtc gtgtatcatt gctcatgttt gaacacctcc agcgcacctt
     4381 gtaaccacgc ttcattttca ggtgttgttt gcagggaaga gatagcttct aaaagttctt
     4441 tttctagcac ttgctgttct tgtcgtatct tcttgatgtc atttccgaca tgaaccatat
     4501 caattggttc ttcttcttcg aaagtatcga catagcgtgg gatatttaag ttatagtcat
     4561 tttctttgat ttcatcaaag gttgccaaat gagcgtattt ctcgacatct tttctttcag
     4621 cgtaggtttc gaggattttt tgaatgtttt cttccgataa tttattctga tttttccctt
     4681 taacaaattc acgactagca tcaatgaata atacatctcg agtttgacga ttcttcttca
     4741 atacaatcac cgtcgttgga atcgatgtac caaagaacaa gttcgcaggc ataccgatca
     4801 cagcataaat actgccgtct tctaataatt tttgacgaat gaccccttct gcagcaccac
     4861 ggaataaaac cccgtgtggc aagacgatag ccatagtccc tgtttccttc aaatgataga
     4921 aaccatgaag aagaaacgca aaatctgctt tggattttgg cgctaatttc ccgtagcgat
     4981 taaaccgaga atcatctaag aaggtcgtat ctgctgacca attagccgaa taaggtggat
     5041 tcatcactac tgcatcaaat gtgtaaggtt catctgttgg ccagtcctta ttcaacgtat
     5101 ccccattgcg gagattcatt tcttcggcat ctaccccatg taaaatcaag ttcatttttg
     5161 ctagattgta tgtcgttgta tttagttctt gcccatgata tttaacgtta tctggatgag
     5221 ttaagtagtt acgtacattt aacatcaagg aacctgaacc catggttggg tcaaagacac
     5281 taaagaatcg tcgttctttt tgatccaacg tcacgatttg tgccatcata tcagaaacca
     5341 tatgaggcgt gtaaaattct ccggctttct tccctgcttc tgaagcaaat tgactgatca
     5401 gaaattcata cgcatccccg atcacatccc catcgtgacc taaaacttcc acgtcattca
     5461 atttcttgat aacttcggta atcgtcacat ttcgttgttg ctcatctgtt cctagttttt
     5521 ttgattggag atccacatca tcaaataacc cattaaattg attgtaggta gatgccaatt
     5581 gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc ttgttttgct tgatccgcta
     5641 agacgttgaa aagatactct ggtgagatcg aataccccaa aatatcaacg attgtggcaa
     5701 tcaggtcttc ttttgattct tcatctgaaa gtaattcacg atacaacatt gtttgtttgc
     5761 ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaaac
     5821 ggtcggataa atatttatag aaaatcagtc ccaataaata atttttatat tctgacgcat
     5881 ccattttact acggagatta tctgccgcac taaataattt ttgatttaat tctgcaccca
     5941 aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatact
     6001 cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga
     6061 cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag
     6121 aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata
     6181 aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta
     6241 tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt
     6301 gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg
     6361 gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa
     6421 aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg
     6481 gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg
     6541 ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca attcttttac
     6601 aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa
     6661 caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta
     6721 tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt
     6781 taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac
     6841 tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac
     6901 aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt
     6961 tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca
     7021 attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta
     7081 gtttaaaag
//
LOCUS       DAJSVD010000126         7061 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSVD010000126 DAJSVD010000000
VERSION     DAJSVD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.129"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..355)
                     /locus_tag="ON498_002483"
     CDS             complement(<1..355)
                     /locus_tag="ON498_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3940708.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQ"
     gene            690..1124
                     /locus_tag="ON498_002484"
     CDS             690..1124
                     /locus_tag="ON498_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3940709.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1147..1467
                     /locus_tag="ON498_002485"
     CDS             1147..1467
                     /locus_tag="ON498_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940710.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1608..1799
                     /locus_tag="ON498_002486"
     CDS             1608..1799
                     /locus_tag="ON498_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940711.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1870..2127)
                     /locus_tag="ON498_002487"
     CDS             complement(1870..2127)
                     /locus_tag="ON498_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3940712.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2285..2548
                     /locus_tag="ON498_002488"
     CDS             2285..2548
                     /locus_tag="ON498_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940713.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2572..2760
                     /locus_tag="ON498_002489"
     CDS             2572..2760
                     /locus_tag="ON498_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940714.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2843..3028)
                     /locus_tag="ON498_002490"
     CDS             complement(2843..3028)
                     /locus_tag="ON498_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940715.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3243..3614
                     /locus_tag="ON498_002491"
     CDS             3243..3614
                     /locus_tag="ON498_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3940716.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3764..3836)
                     /locus_tag="ON498_002492"
     tRNA            complement(3764..3836)
                     /locus_tag="ON498_002492"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3801..3803),aa:Gln,seq:ctg)
     gene            complement(3882..4310)
                     /locus_tag="ON498_002493"
     CDS             complement(3882..4310)
                     /locus_tag="ON498_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940717.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4370..5128)
                     /locus_tag="ON498_002494"
     CDS             complement(4370..5128)
                     /locus_tag="ON498_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3940718.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5423..5620
                     /locus_tag="ON498_002495"
     CDS             5423..5620
                     /locus_tag="ON498_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940719.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5726..6208
                     /locus_tag="ON498_002496"
     CDS             5726..6208
                     /locus_tag="ON498_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940720.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6266..6862)
                     /locus_tag="ON498_002497"
     CDS             complement(6266..6862)
                     /locus_tag="ON498_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3940721.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2364 a         1091 c         1400 g         2206 t
ORIGIN      
        1 cttgtgtttc cgtttgaacc gtttcttgta caacaggttg ttcagcttga acaggttcag
       61 cttggactgg tgcttcttga acggctgctt gaggttcagc agctgcttgt gcagagcctt
      121 ctgttgggat tgttaattgt tgtcctgagt agatcaggtt aatatcagaa attgaatttg
      181 attctgcaat ggcattgatc aaggagttat caccaacgta tttttgagaa atcgttgata
      241 gagtatcacc tgattgaacg gtataagctt cgtccgcatg agcagtcgtt cccatgaaga
      301 atgcagcgcc ggcagccaaa gttgttccaa aaagtaaagt tttaagtgat gtcatgaaaa
      361 atctccttta ttcgttaaaa agttttattt gttgtgttca acgaggaata ctttaacacg
      421 tgaaaatatt tcataggtaa aagaagcatg acaatttatg acaaatccac gttttttgta
      481 acagaatgat gttcgctcga tgtgaaaaat gatgaggaat ttcacataat gcagaaataa
      541 tgggattaga acaggtgttc tgatgagaaa agaaatgtat aaataacttg aaagaaattg
      601 cttttgttca tcattttatt tacatattgc gtatattttt tgtatttttg cttgagatgt
      661 tacaataaaa gaaaaaggag gcggttccaa tgagtaaaag ataccagaac atcatggtag
      721 cagttgacgg atcaagacaa tcgatcaaag cgttcgctga agcccttgac ttagcaaagg
      781 acaatgaagc ccacttattt atcgtgtcaa tcatcaacaa agttgagctg acccatagtg
      841 cctatgcctt ttcaaaaatc tatgcagatg aaaaacaaag aacagaagta gccatgctga
      901 aaaaaatcaa tgatgccaaa gagtacggca ttgctgagat ccacgcaata gttgaaactg
      961 gagatccaag aaatttgatt gccaatgtca tccctggaca agaaaatatt gatttgatcg
     1021 ttatgggagc gacaggaaaa ggtgctatac aacaagcatt agttggatcg accgcttctt
     1081 atgtcgtatc acatgcccct tgcagtgtgt tggtcgtgaa gtaatgagat aagggaggaa
     1141 tagaaaatgg ttcgtcctca agaagtccgt gcgccaaaag aaaaaataga aattttagca
     1201 attattgaag acggcacaca aacaaagaag ggatactcga tcgctttagt aaagtggaaa
     1261 ggaaaaaaag gtgttgctct gagatgggat ggagataacc aacaagataa aggattccct
     1321 gtcacagcta acggttatca tccagcgtgg tttgttctgc ctgataaatt tatggaatta
     1381 tacagctatg attatgcaag agccgcagca tcaatcaaag cattagataa attaacagaa
     1441 gagcagaatg aaatggagga aaaataaaaa agacgaacca atcagccttt agaaaactga
     1501 ttagttcgag gagtaaaaat gaaaaaaggt tatttgggat gaaactagaa taatgcgtat
     1561 aacttaaata aaacttaaag caataaaatg gcaagagggt gaggcaaatg gaagcaggaa
     1621 aagtattaca ggaagtaaaa gcactatgtg cagataaaaa atatgaagaa gcgaaaagcc
     1681 tgatcgaaaa gaacaaagaa aatttggaag aaaagtaccc gatcgcacaa gaatttatag
     1741 aattaaaaca aggaacgatc cttcaccgat tcaaacattt ttttggtata acagaataga
     1801 aaaaacgatt aaagatcggc agaaatttgc tgcatcttta atcgttttct ttttaaacat
     1861 gcgagggatt caattctgat tcttatataa aaaatagatc caaataataa gaaaaattac
     1921 tgaaagcgtc aattcgatga tgtgtgttat taatacagac gttgtttccc aatgcctaaa
     1981 agaaaaaatt tgataagtaa aatatacagc aggtaaagag gccagtaaaa ctgaaaactt
     2041 cttttttgtc cattttatgt aatagaaaag aatgcttatc ataataaaaa aaggaatata
     2101 aaaaacaaat tgaccgatcg cgatcatact attgcctccg ataaataaag tccatgatac
     2161 aaaataacac tttgtgcgaa atggcgcaag cagttttgtg gatttatccg gtaaaatctg
     2221 tatcgatttt gaaattagag gagaaacttg ctataatcaa agtgaaagaa ggagtgggat
     2281 tttcatggat catgtagtgt tagaaatcga tttgcatttg atcaataata ttcgggtgat
     2341 ttactatgtt gtaagtaaca gtgtcgaaca acgtgtactg acgaataaaa taaatggtat
     2401 tttagcaaag aaagacgttc accgattcaa taatggtgaa ggaagttatt atagtatccc
     2461 agtagaaaaa attatttata cgacagtcaa agtcagagaa gatctagata ccaaaaaagc
     2521 atatgaacct atttttacaa cttattgatt gacagcagat ggaggaaaaa aatgaatcca
     2581 tatgaacaac ttattcaaga tttgatcgat ggaaaaatcg aaaagattga tgtaaaaaga
     2641 gaagaattaa tggcttttcg agaagcgtgg ctgaagttgg agaatcgtaa atatgtggtg
     2701 ggaaaagcct cactgaacgg caatgtgact tatcattacg atccgacacg tttattttag
     2761 tatgaagtat tcagaaaaga accaaaggag gacgatgcag cagcattgtc ctcctttgat
     2821 tttacaaaag tgaaagtttt acttatttga atagattttt aaatttgttt ttgacactgt
     2881 cgatatttac atcttttaaa agattttggg ctttctcttt ataaggtcct aattgttctt
     2941 tatgttcttc gataaatcgt tttgcgccat cgaaattgcc gttcttaatc atttccttga
     3001 ctttttgaaa taattcttct tgattcattt tgaacacatc ctttgctatc aacatgattt
     3061 tttacatgga caaaacgaga aaaactgatt tatccacacg tcaataatat caaataacga
     3121 tcatttttac taaaaaaaca ctcgattctc gcttctaaag taggagaaaa atctccaact
     3181 tgggacggat ggaatttctt taactgaccc atatactgaa tatataacaa ggaggtagaa
     3241 agatgaaagt gattaaatat ttgattggac tggcggcgat tagtggtatt ttgctatttg
     3301 gactgaaata ttatactgaa ggaagttatg gagaaatccc tggtatactt gatcagctaa
     3361 atccattagt tgaaaaagga gaggtctatg taaagacaca aaaaccagag gaagtaaatg
     3421 aatatggtac agcaaggtat gtgcagaaag ctgctgatgc caatgggaaa gaacgaacgg
     3481 ttgaatttaa tggtctttct gtgttaaaag aaaatcatta tttgaaaatt agcaataaag
     3541 gagcacacat agaaacgtat gaggaagttc ctagagaaaa agttccggag aaagcattag
     3601 caattattgg gtaaatcagt ttttgtatta agaatacgcc cgattttatt attcataatt
     3661 taattatgta aactaaatac aagtagtaaa aataatacaa taatccccct gcaaaaagat
     3721 tgttctttta cagggggttc attttttcag caagcacatc aaattgggat ggcagggttc
     3781 gaacctacaa ttgcttgatt cagagtcaag tgccttacca gtttggctac atcccattta
     3841 ttcaacaaaa taatcatagc actttcctaa aaaaacgcaa gttatttttc ttgatcggat
     3901 agttgttgct ttactcgtaa aaggcggcgt actagtttct tttctaatcc agcaggaagg
     3961 gcaatcagtt ctgctactga caagacggtg tccaataact gatattcttt gacaggttca
     4021 ttcataatat gatagatgtt tgctaaacgt tcaatatatt caaagcgagt atactctgtt
     4081 cgagcaatta gaaatttcaa taaatcggtg gctttctcgt actcttgatc tgcgtaataa
     4141 acaagagatt gacgatacaa tttgatttcc agttcattga gggtagcagg tctgcgacgc
     4201 tcgttccact cattaataga gactttacct tgaacatttc ccttgcagtc acggacaaaa
     4261 ataatcggaa tttcatttgg atccattttt ttatgtgtga atttttccat tatctacgct
     4321 cccttcaaag gtttggtatc ttgagtataa gggatttttg tttgaattac tagcttttct
     4381 ccaaagttag catctgtcca cccattgcag tgaaaatcgc cgaaaaatcg ttgatcggta
     4441 ctgctcgatt ttttacacca tagggatcgt tgacataaat attttctttg tcatatccag
     4501 tgactacaca agcatgaaca agaggagtga ctcttacttc tccattattt gtggtccaag
     4561 tcataaaatc agtttcatct gggacttcaa aattgatagt cacagaagtc cagacaggat
     4621 ttcctttaga tagtgctttt ttgatatcat cgagttgagc atcggttttt ggaaaagtgt
     4681 gataatccgt ttggacgatt tgctgagcaa gggaggcaac tgggggaaca tagacgacca
     4741 tagcccagtc tccgccactg atatcaccaa caaatccttc attaggatcg ccatgagtcc
     4801 catctgcatt gaaaacaggg acgtaatcca attgctcagc taactggttt ttagttgttt
     4861 cataaccata aaaagataaa agcatagaaa gcgaagtgac ttcacagcca ttttctaaag
     4921 aaggagaatc aaactggctc tctaatggaa catctagtaa aacttctgct ttctcttttg
     4981 tctttttcgt tttttgtgtt gaactttttt gtgcagtgtt cattgtatgt tctggagaat
     5041 ggatatgtag gtaataactt gtaccgctta ataaaagagc agctgtgccc cctagaaaca
     5101 atagtttttt gaacgttttt ttcttcattt gaaatctccc ttttcagctg tttatctttc
     5161 ctattataaa tgtattttta ttggaaatgg aagtattata ttttaatatg taggaaataa
     5221 ttagttttta aggaatatgt tttaattttt tgaaaaaaat tcttctaaat acaacagctg
     5281 ttttaattgt gaaaacgaaa acagtacgct ataatgcctg taaggttgat ttttaactgt
     5341 aaataaaaat caatgagtta aatctccaaa gaaatagcag tggtaatgtg gagaaaagtt
     5401 ccagcagttt aggaggattt tgatgacgga agaaatgatt aatttaggag aacaatatgc
     5461 atgcaaacca attggtttta caaaaacggt cattggtgaa gttgtatcaa aaatgacaaa
     5521 ctgcgccgtg gtcaaagttg cccaatgtgc tgcagaggac caagaattat tagatgaaaa
     5581 agcaagcatg gttgttgcaa aatatgatac atttgaataa agaaaacaat tggacgtgcg
     5641 gcgtgctgca agtccaattt ctttgagaag gtaaatcagt attcttttcc tattttctgg
     5701 agaagaaaaa gcgaggcgag ttgtcatggt aacattcttt acggttctat tgtttttatt
     5761 cgttgttgtg atgtttttat ttctttggct ggttcgaaaa gaaattattt atcggactgt
     5821 gcgaaatcga tgggtctact tggtgatacc cttcttagtg tcactagtca tttggtatac
     5881 actcatcagt catcctacag cagatgaatt ggcaaaaggg atcttgtcag caatgatttt
     5941 tattagtttt ttgttggaca gtagaggaat cacagaagaa ggacttgttt tgaatagctt
     6001 tgacaaaaaa ggtgttcctt tttctgagat caataaaatt gtattatatc agccaaaagg
     6061 ttctaaaatc gtaaaaatga attttttccg aaacggatgg cgaggaccga tgctgaaatt
     6121 ttctgcatca ttagaagaat tagttccatt tttgtcccaa cgcttaaacg atgaggcaga
     6181 gattgatatt atgattgatc cagaataaaa acagaggttg cgacaagatg tttgtcacaa
     6241 cctctgtttt ttatttcaaa taaaattatc caaatacgcg gaagcgatcg gcatcgttca
     6301 tgtattcttt ttccccagcc ggtgtttctg gtgaaccgaa tactaattgc gaacgtagtt
     6361 tccagtttga agggatattc cattcttttg caactgcttc gtcaataact gggttgtaat
     6421 gttgcaagtt tgcgcccaat cctttatcaa ctaatgctac ccaagtatta gctgttgcga
     6481 ttccgtttga ttgttctgac caatctggga agttgtctgc atataattca aattgttctt
     6541 gtagaccttt taccacgtca gtatctttga agaataatac tgtaccataa ccatttttga
     6601 aaccagctaa tttgttttgt gtatttggga aagcttctgc tggagtcaat gggcgaagtg
     6661 cttcttcagt gatttcccaa agtttttcat gtgcatctcc gaaaaggata actgcgcgtg
     6721 ttgattgagc attgaaagca gttgggcttt ctttaattgc ttctttgatc aaagttgtta
     6781 attcttcatt agaaagtgaa acattacgtc ctagatgata gattgaacgg cggtttttca
     6841 aagtgtcagt aaaagttgtc ataataaaat ttctcctttg atttgcgttt tgcatttttt
     6901 taattgatta cgaaatgaag tatagcacct tacaaaaagt aagtaaacag atatgctcac
     6961 aaaaaataag cgaagttcaa aaaacaattt taataagaaa aatcgttatg taaggatttt
     7021 cttagttatc gctttcattg ttgtgtttta ggaatttttg c
//
LOCUS       DAJSVD010000127         6935 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSVD010000127 DAJSVD010000000
VERSION     DAJSVD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.130"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1..1151)
                     /locus_tag="ON498_002498"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="ON498_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="ON498_002499"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="ON498_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR3940722.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="ON498_002500"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="ON498_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="ON498_002501"
     CDS             complement(2435..2665)
                     /locus_tag="ON498_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HCR3940723.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="ON498_002502"
     CDS             complement(2895..3713)
                     /locus_tag="ON498_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3940724.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="ON498_002503"
     CDS             3874..4563
                     /locus_tag="ON498_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3940725.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="ON498_002504"
     CDS             4577..6079
                     /locus_tag="ON498_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3940726.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="ON498_002505"
     CDS             6092..6568
                     /locus_tag="ON498_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3940727.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="ON498_002506"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="ON498_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2326 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DAJSVD010000128         6817 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSVD010000128 DAJSVD010000000
VERSION     DAJSVD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.131"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            49..939
                     /locus_tag="ON498_002507"
     CDS             49..939
                     /locus_tag="ON498_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR3940728.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1012..1836)
                     /gene="nadE"
                     /locus_tag="ON498_002508"
     CDS             complement(1012..1836)
                     /gene="nadE"
                     /locus_tag="ON498_002508"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3940729.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1848..3320)
                     /locus_tag="ON498_002509"
     CDS             complement(1848..3320)
                     /locus_tag="ON498_002509"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3940730.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3957..4319)
                     /locus_tag="ON498_002510"
     CDS             complement(3957..4319)
                     /locus_tag="ON498_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3940731.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4542..5294
                     /locus_tag="ON498_002511"
     CDS             4542..5294
                     /locus_tag="ON498_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3940732.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5434..6357)
                     /locus_tag="ON498_002512"
     CDS             complement(5434..6357)
                     /locus_tag="ON498_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3940733.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6372..6644)
                     /locus_tag="ON498_002513"
     CDS             complement(6372..6644)
                     /locus_tag="ON498_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940734.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2089 a         1353 c         1163 g         2212 t
ORIGIN      
        1 taacaaatat gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa
       61 ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc
      121 atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg
      181 agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca
      241 gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tattagtttt
      301 ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat
      361 acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa
      421 gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt
      481 accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag
      541 atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct
      601 tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat
      661 tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac
      721 ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg
      781 tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa
      841 gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat
      901 tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat
      961 aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca
     1021 gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc
     1081 ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc
     1141 attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc
     1201 tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt
     1261 cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt
     1321 cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct
     1381 ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt
     1441 aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg
     1501 tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc
     1561 ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc
     1621 ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga
     1681 atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata
     1741 tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac
     1801 acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta
     1861 tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat
     1921 tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat
     1981 agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa
     2041 actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca
     2101 gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg
     2161 tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg
     2221 cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc
     2281 gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg
     2341 gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct
     2401 ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt
     2461 gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata
     2521 tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg
     2581 cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag
     2641 tctttatggg ttttagcgta ggccataaat gcttcataat catttccata agattggact
     2701 aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg
     2761 cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt
     2821 tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata
     2881 cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct
     2941 actaattggc agtgcgctaa aggcccttcc acttggatca atggttcatt tgcaaaaact
     3001 agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc
     3061 aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac
     3121 gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg
     3181 ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat
     3241 tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg
     3301 tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca
     3361 ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt
     3421 tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg
     3481 tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa
     3541 ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag
     3601 ccgttaagat accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg
     3661 caaaagtttt tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg
     3721 gatataactt attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt
     3781 gcgttgaaaa aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa
     3841 gcagctgtca atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt
     3901 ttattgtata cattagatat ggagcaggtt accataaaga tgattattag tcgttattaa
     3961 atagaatcct tcgtattttt ttgttctatc actacaatat ctttatttgg acgtttgaat
     4021 tgtattccgg cacgttctag ctcctgtttc actcctacat ggactttgac atcttctcgt
     4081 gtcattttta aaacgtgatg aatcgcatag tcgatttctt gccttgtgat ttttttcttc
     4141 gtattgatca tcacgacttg ctttttatca tatgtgtcta ccaatagcag ttgtgttcga
     4201 tctaacgaaa ataatttgta ataattaata gccgtattcc caaaaaagaa ttccactact
     4261 ttaggataaa atgtcttaat atttaaatta ctattcacag gtgtttcgat tagcttcatc
     4321 tcattcctct tttctatata gtcgatttag atccaatctt aagttatgtt accatctttc
     4381 ctagcattaa ggaatcggtt acaattgaac ctttaaagac aaaaggattt accttttatc
     4441 acttaatagt ataacataat ttcatgagaa aaaaatacaa ttttttcttg accatctcgt
     4501 tacgtaaagg attatgctta catttgtcag gaggaaaaat aatggagtac acaataaaga
     4561 aaatgtcaga gatatcagga gtcagtccga gaaccttgcg cttttatgat gagattggac
     4621 tgctaaaacc agctaggatc aattcttcag gttatcggat ttatggcaaa aaagaagtcg
     4681 atcgtctgca acacatctta ttttatcgaa caatggcatt caagttggat gatatccaag
     4741 aggtcttaga caatccatct tttgaccacc agaaagcttt aatcaagcat cagcaaatgt
     4801 tgctggaaaa acgggcacaa atcgataccc tcttgacaac tgtccaacag acacttgata
     4861 tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa
     4921 agttgaaaga aaatgaagaa tcctatggac aagagattcg acaaaaatac ggaaaaaaaa
     4981 aggttacagc atccaatgaa aaatggatga atatggataa agaaacgtat gaagaaatgc
     5041 aaactgttga aaagcagatg ttatttgatc tcactcttta cttaaaagaa ccagaagatg
     5101 aaagcttagc agaccgtatc ttccaagctc ataagaagtg gttatcttac agctggcctg
     5161 aatatcagcc aaaagcgcat aaaggactag gaatgatgta cgaagccgat gaacggttca
     5221 ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa agctttaaat gaaattatcc
     5281 aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt acacgtaaaa tgacctaata
     5341 acaaaacagc agacacccaa gttagatttt tatgctaact tttggtgtct gctccattct
     5401 ttcacaagtt ttttcatcct actttaaata tattcatcgt tgaataaacg tataaggtat
     5461 tttcacttta tccttttttt ctgtaagaaa caattgaaat gccatttctc cgcaagcttc
     5521 cagatggtgg tcgatggttg ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc
     5581 aatcaccact ggtgtcttat cttgcagata gctttgcaag atgcctgctg caaccatatc
     5641 cgaatttgta aagacaccat caactccaag ctctttaaaa aagaagcctg cttgttttcc
     5701 tttctctgca tctatacaat cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg
     5761 atgacatagc tgcaaagtga tttttgagtt ataacttagc ctgccacttc gccccaacgt
     5821 gatgcccaat tgctttatac cttgctgctt caaataagta agaccttctg taattgattg
     5881 ttctcgatca ataaagacac agcttgcctg ttttcccttg atttcttcac aaaaaactag
     5941 aggtccatat ttttgatatg ccaataaatc atctagtgta ttggcacgag acgtaatgat
     6001 caatccatcg aaagctttag ctgcaaattc ttcaagatac cttcgctctt tttctttctg
     6061 atagcccgtc ggtaaaagtg tcactttgta atcataatca aacgctgctt caatgatccc
     6121 actgatcaac tgatcaaaat acggatgatt ggtataaggc agaatcactc caatgctttt
     6181 cgtttttcca taacttaact gtctggcatt tgcattaggg atataatcga cttctttcat
     6241 gaccgcaagt atttctttcc ttttttcctc atctacatat ggatgtccat tgattacacg
     6301 tgaaatagta gaaactgaat aacctgtgag ctttgcgata tctcgaatgt ttgccactgc
     6361 tgcttcgcca cctatacaaa aagattttct tgtaaatagt ctaccacagc tggaccgcat
     6421 tcaaaaaact gatacatttt tccttcatgc gaaaaactga tccgctgccc gccccaatat
     6481 atatcttttg tcaaattttc gatttcattc attggtattt cttttactat attcctattc
     6541 aacccttgaa aactgtacaa cgttaatacg gatcccttga actcgacaaa cgcataaaaa
     6601 taattgttcg ttgtgccatc tgttatctct acataagtat tcattgcaag ccctcctttt
     6661 gatactttga gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga
     6721 aaagttttat tcctctttca ttattaccaa agtctcttat tttaaattga atacattata
     6781 atgatactgt aaatatatct ttattattag tgaatgg
//
LOCUS       DAJSVD010000129         6431 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSVD010000129 DAJSVD010000000
VERSION     DAJSVD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.132"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            26..847
                     /locus_tag="ON498_002514"
     CDS             26..847
                     /locus_tag="ON498_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3940735.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            867..1427
                     /locus_tag="ON498_002515"
     CDS             867..1427
                     /locus_tag="ON498_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HCR3940736.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2181..2948
                     /locus_tag="ON498_002516"
     CDS             2181..2948
                     /locus_tag="ON498_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HCR3940737.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2974..3519
                     /locus_tag="ON498_002517"
     CDS             2974..3519
                     /locus_tag="ON498_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940738.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3520..4086
                     /locus_tag="ON498_002518"
     CDS             3520..4086
                     /locus_tag="ON498_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940739.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4101..4349
                     /locus_tag="ON498_002519"
     CDS             4101..4349
                     /locus_tag="ON498_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940740.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4368..4607
                     /locus_tag="ON498_002520"
     CDS             4368..4607
                     /locus_tag="ON498_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940741.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4624..5208
                     /locus_tag="ON498_002521"
     CDS             4624..5208
                     /locus_tag="ON498_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940742.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5315..5596
                     /locus_tag="ON498_002522"
     CDS             5315..5596
                     /locus_tag="ON498_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3940743.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5586..5927
                     /locus_tag="ON498_002523"
     CDS             5586..5927
                     /locus_tag="ON498_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3940744.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2434 a          814 c         1335 g         1848 t
ORIGIN      
        1 attaattaaa aaggatgaga ttataatggg gttactggtt ccgagagcta atacttatac
       61 tataccttta acgaaacgcc aattaattgc taaaagatat caaaggatta ctagagccat
      121 caatagagag ttttggaatt ctgaaagtga tactgctcac agtttatacg taggttctta
      181 tgggagagga acggcaatta gtactagtga tatagatata atagttgaat taccgatggc
      241 agaatttgac agattcaaaa attatttatc aaatggaccg tctaagttat tacaagttat
      301 aaaaaacgca tttcaggaaa tactaccaaa tagtgatatt agagctgatg gacaagttgt
      361 taaaataaat ttccatgatg gaatcaaatt tgaaatagtt ccagctttta atgaaaaaga
      421 ttatttgggt gagtcaaaag ggtttattta tccagattca aacatgggag ggaactggaa
      481 agctacaaat cccaaaaaag agcaagaagc catgaaatta aagaatacaa aaagtaataa
      541 tttattatat gctacgtgta aacattttcg acatgtacga gatacagaat ttacgagtta
      601 tcatctttca ggaatagtaa ttgacagttt cgtatatgag gctatgggaa attggaaatt
      661 cgttgaaaat aacagtgggg gtcaaaacat tagttctgta tcttatgaga cagctttatt
      721 agaatactat aactcacata aagttatggg cggtttaaat ttatactctc ctggaagcaa
      781 tcaatttgtt aattctgata gtagtatcat atgtttagaa aaagtattaa aaaaaatagc
      841 tttataaagt tttatggagg aacaacatgg gagacgaaaa aaagcgcttg gaagttcaat
      901 tacgtgaagc atatggacga gtaacgtata cttacaccac tcatttaaag caagccgata
      961 atattatttc tgacaataaa attttaaggt gtcttcaaat tggtttgtca gcccttacta
     1021 ctggaggggt acttagtacc attattactg ataatagtga gttaaccttt gttgctggat
     1081 tatgttcaac aatttctctt ggattaaact tatactttaa gaattttgat cttgctgaag
     1141 aagctaaaaa tcatcaaatt gttgccgata aattatggtt gatcagagaa gaatacgtaa
     1201 gcttactaac agatttaaat cgtttaagtg ttgaagatat tgtgaaaaaa agagatcaat
     1261 taatgaacaa aactcatgaa gcatataaaa atgcccctaa aacaaatagt aaaagctatg
     1321 aatcagctca gaaagcatta aaatctgaag aagagcaatt ttttacagaa gaggaattgg
     1381 ataagatgtt accagagcat cttagaaaaa agagttctaa atattgaact ctttttttat
     1441 gcttaaaatt ttcttgtaat ttttctattc aatcaataaa taatctatat ttaagaagaa
     1501 atatgatgat agaaacatct cctaaagcca ccgcaagccg tcctagccat tgcccagtcc
     1561 gacatcgcta gattagtgca tctctccaaa aaagtttcct aaagccaccg caaaccgtcc
     1621 tagccatcgc ctagtgccta gtccgatttt gcttgatcgg ttccatttct gtttatttag
     1681 tgggtcaaca ccgtaatctt aaatagccat cgcctattta agattactag gtctgctttg
     1741 aaggtaaaaa gtctttcatt cttcaagact tttaggcttt caagctacgc ctgaaagcat
     1801 tgaaattcat cgctctttat ttggttttgc catttataat tccttacgcc cattatcttt
     1861 ttcgcctgtc ataccttgtc agaagtgttt agagcttatc tcatctttgg attaatacaa
     1921 gtggcgtaaa tttttttcta aacgcaagcg ttacgaaaat aatttactta aacacttgta
     1981 ttaatccggc agataagaac gcattctaat cactcccgac aaggcaagac aggcgaaaaa
     2041 gataaataaa tgaacgaaag gaagaaacat aaaatgcaaa accaaatagc gatgaagaaa
     2101 aaaccacctc cctctaaaac aatccaaaca aaaaagaatt ttgaaaaacc aaaaatcaaa
     2161 aacagaaaga agcgttcaac atgagagata gaattttaga agttaatcaa gaaatggtaa
     2221 acaagttaac aaatatcagt gggaactatg aaaagtacga tggtacttta cttgataatt
     2281 atatttttca cgatacacaa aatatcagaa ttggcagaag taaaccgaga gcgttcattc
     2341 tcatcaaaga gaaatatttg aatgaatgga atagcaccta tgaaatggtt atgactgata
     2401 actttgaaaa ggtattggat ttcagaaatg ctttgttggg ttctgatatg ggaaatagca
     2461 gtcttgaatc tattgaagtt gaaaacaaga ccgagaatga aggtgaagga ttgattaaga
     2521 atgtcctgaa aatcgaacaa aaatatatca gtgaagatag aagtttttat agagtcacaa
     2581 gctcggttgg tctagcaaac tttgctacta acttaattgg aaacaatgct agtgaatctt
     2641 tgctagtgat gtgtctgaat acaaagaatg aggtcattgc ttatagcgaa gttttcaaag
     2701 gtggaattaa tcaatcagta gcgataccaa gagagatttt tcaactagct ttgctaaaca
     2761 attcagcaaa aatcgcagta tcacataatc atccgagcgg atatgaattt ccaagtgaaa
     2821 atgacaaaag ttttactacg aatttgaaaa acgcaggaaa aacaattggt attgaattgt
     2881 tagaccatat catcgtaaca agtaaaaaca caagctatta ctcatttcaa gaaaatggtt
     2941 ctttataaaa attagagaaa agaggaaaac aagatgggcg aaagaacaca agcgttaatt
     3001 acagtcaaat atggtaacag caaaacgaaa gtaagcgtgt caattcatta tcagtgggga
     3061 tatggtcgat tcatgttgat ggacgttttg aatcaaggaa tatatttaat caataaagca
     3121 tatgacttac gagatgaatc aagagaagaa ttagcaaaag gaatctttga tagaagtaca
     3181 ggagttatca attttgtcac ctatgaatct ggagtgagtg cttatgatga aacagggttt
     3241 ggtgggcgct atgaacctta tcaagaaacg attgaagatg tgatgaagtt ttcagataac
     3301 aatgatggct atgctcattt agagatggtg ttcgatgaaa atggatttag tctagatgat
     3361 aaaaccagat tgcgattgtt tgattttgag ggtcaacaga taggactaaa caagttttgt
     3421 gaaagcggaa aagagtttgc tacgaaagat tttattaagg gatacagatt attgctagaa
     3481 aattatggaa taaacctaga agaagagagc gtgaaataag tgtttgaaaa aagaaaagac
     3541 cagttcccta atttagaagc gaaaagtcaa aaagtgattg atggtgaaat gattgaatta
     3601 ttccaaaaaa gttatggaac catagaaact accgcaagtt gcaaatacta ctttcaagga
     3661 aagagaaaca cgtttaaagg ctttgccgat tctgatgaag aattggcaaa gaaaatcatt
     3721 gattggcttg tcagcagtga acacgtagca agagaagata ccgttagaac aacgattgag
     3781 ggtgaagtcc taacaggtac tagatgtgtt tgttgtaatc atgaatttgt agaagatgaa
     3841 ggttgctacc gaatcaatga atatggtgat tttatttgcc agagttgcca cgagatagaa
     3901 tttccaaccg aaagaagttg gtttgaatac atgctacaag attatgagat ggaaacagaa
     3961 aatggtgaag tagttttttg taccaaagag gaattgagta agagaagcga tgatgaaatt
     4021 gaagattggt gtgtgtggtt agtagggaca gatgattgcg ttgcatatta cacaacagca
     4081 gaataggagt gtgaatcagc atggaaggaa tggattttat agatcatgaa gatttgattg
     4141 attttggtta tacatggaaa ggaatggtag gtattagcag aagtcttgct aatgcctttt
     4201 acgaaagaaa ttatgcagtc tatgtccttt atgatgacaa tacagaaagc cttgttgatg
     4261 aagagtataa attggattta gagaatgttt tatatgggat agaaaaagaa gatttagcaa
     4321 aatatatttt ttcatggttg ggtcaataaa cgaaagaggg tagcaacatg acaaaattca
     4381 aaagtaaagg tatgcaatgg tgttcttatt attatgttgg gaatgatgaa gagcttttga
     4441 gaaaagccaa acaaaaaggt tatccagata agatgttaaa acagattggc tattacatgc
     4501 cagaagagag aatagaaaaa gaatcttgta aggttctttt tgatagtgaa cagctattag
     4561 ttttgttaac tcaaaataaa ttgatttgtc aatttgaaaa taattaatat ggggtgaaga
     4621 accatgaatg agaataagat ggtattaagt gaatacgcaa gattgagaac cattaattgg
     4681 ttgaatggta tgcaaagtag tggcgctttt gatggatttg atgaggagaa tatggacttt
     4741 gaaagtttat cgaatgagga tattagatat tattttatcg aatggtattt tacagaacca
     4801 gatgaagagg tggaagatga accagaatta attttagaac gtcaaagaat tttattcagt
     4861 gaacaagata gtaagaggtt gtatggttta tatgacttac aactgaaagg attaaaagcg
     4921 cagtatgttg ctgatagtta cgatgatatg gtagaacagg gggcagatag ggttgtcact
     4981 tattcagaag atgatgaaga ggaatgggaa aactattcag atgatgagat atgctttaat
     5041 gatgggtata tgcctattga aatcaccgaa gaagaatatg ataaagtgtc ggatagcgat
     5101 gagattggta tgtgtacggt tgtaagtgga ttggatatag atgtccaaag agtagaggtt
     5161 gcataccaga agatgaaaga acgtataaaa agtaaagtag aatcttgatt tgattggatt
     5221 gtttgcaaag tagctaatta agagtataat ataatcataa atgtagttaa tggatataat
     5281 aatgagtata ttatactcaa aagaggtgga cgaaatgaag gtaaaaaatc caaaagaaaa
     5341 aagcagaatt caagtcggaa tagacaagga tttaaaagaa aacgcagaaa tgatattgga
     5401 agagctaggg ttaaatccaa caacagcaat cacaatcctt tataagcaag tggtcgctag
     5461 aggtgagttt ccagtagaga taaagctaag cgaagaggaa aagcaaggaa tcagattgca
     5521 acagctaact atggatatgc cagttgatgt tctagataca gatgaaaagt tagaagagtg
     5581 gttcaatgaa gcctaatgag atagtaacgg tctatgttgc atttacagat aaaaatggtg
     5641 gtaaaagaag acctattttg gtggttagtg ataaagaaga tagagtagaa ttttttggaa
     5701 tcacaagtca atatgagaat aaatcagacc aaataaagag agtgtatttt cctattgcag
     5761 aatgggaaaa agtaggtcta aagaagcaat catggattga cgtagggtct ttaaaggcta
     5821 ttcccaaaag ccgagagaac atatcattta aaaaggttgg tatgctatca gctattgata
     5881 cgaaaaagct aaataggttt atcgagagat tacaaagaaa agtctaacaa ggcttttctt
     5941 tttttgttta tatgtatatt aagatatacg tttatataaa cgtatttatg tatattaaaa
     6001 tacacgtaaa tatatacgtg attataaaaa ttaagaaaat attaagtatt gttaaatagt
     6061 gataattagt aatatacgaa ttataagata cattttatat ttaatgtatg taaataaata
     6121 aaatgactga cggatattga atagtaataa ataacaataa ttcagacgga tacagtgagt
     6181 gaagtagctt gctacgaacg aacaggcaga atgaaacggt agtttcatta gagcaagtcg
     6241 cttgcgacgt agcgttacca ctgatgtaac tggcaagtta catagagaga gtttgcttgc
     6301 aaacgaacgt tacccacttg aatagaaaac aaaaataaca tattagtgaa ttttgaatag
     6361 gagccacaac tcaaccaacc gcttgcggtg ggtgagtccc acggaggaaa agacgaacga
     6421 acggtcgcgt a
//
LOCUS       DAJSVD010000130         6344 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSVD010000130 DAJSVD010000000
VERSION     DAJSVD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.133"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(99..1229)
                     /locus_tag="ON498_002524"
     CDS             complement(99..1229)
                     /locus_tag="ON498_002524"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3940745.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ON498_002525"
     CDS             complement(1878..3080)
                     /locus_tag="ON498_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3940746.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ON498_002526"
     CDS             complement(3140..3343)
                     /locus_tag="ON498_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR3940747.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="ON498_002527"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="ON498_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="ON498_002528"
     CDS             4880..5692
                     /locus_tag="ON498_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940748.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1894 a         1300 c         1001 g         2149 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aaga
//
LOCUS       DAJSVD010000131         6339 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSVD010000131 DAJSVD010000000
VERSION     DAJSVD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.134"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..176)
                     /locus_tag="ON498_002529"
     CDS             complement(<1..176)
                     /locus_tag="ON498_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3940749.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWG"
     gene            complement(287..766)
                     /locus_tag="ON498_002530"
                     /pseudo
     CDS             complement(287..766)
                     /locus_tag="ON498_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            complement(907..1509)
                     /locus_tag="ON498_002531"
     CDS             complement(907..1509)
                     /locus_tag="ON498_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3940750.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1754..1960
                     /locus_tag="ON498_002532"
     CDS             1754..1960
                     /locus_tag="ON498_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940751.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2272..2991
                     /locus_tag="ON498_002533"
     CDS             2272..2991
                     /locus_tag="ON498_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3940752.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3072..3755)
                     /locus_tag="ON498_002534"
     CDS             complement(3072..3755)
                     /locus_tag="ON498_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR3940753.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3973..6159
                     /locus_tag="ON498_002535"
     CDS             3973..6159
                     /locus_tag="ON498_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3940754.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2347 a         1067 c         1197 g         1728 t
ORIGIN      
        1 ccccatataa cacaagcaat aatcactgta tcgcgttgct tcagttgatc agtatttctt
       61 cggtttttaa tcttatctgg tacacaattg tggtatatgt tagaaacagt tctcataatt
      121 tccttgaatg ttataaaagt atctgtataa tgttcagtat attttgatga acgcatatgt
      181 agtcctcatt tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac
      241 atgtttagac attttcctct aggaaaaaat taactagcac cacgggtatg atatagtatg
      301 taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag
      361 tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa
      421 tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat
      481 cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt
      541 aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag
      601 caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa
      661 tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac
      721 aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc
      781 attgattttt accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt
      841 ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt
      901 aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt
      961 aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta
     1021 agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt
     1081 taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata
     1141 gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt
     1201 caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag
     1261 acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga
     1321 aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac
     1381 cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac
     1441 catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt
     1501 atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc
     1561 ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt
     1621 cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg
     1681 acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga
     1741 tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa
     1801 atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt
     1861 gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg
     1921 gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga
     1981 gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa
     2041 cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt
     2101 ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga
     2161 gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt
     2221 atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata
     2281 attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt
     2341 gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg
     2401 ctattttatt ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa
     2461 cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc
     2521 tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa
     2581 gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca
     2641 caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga
     2701 atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac
     2761 tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa
     2821 atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc
     2881 gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc
     2941 cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac
     3001 taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg
     3061 attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc
     3121 tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac
     3181 agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga
     3241 tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc
     3301 gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt
     3361 ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg
     3421 ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga
     3481 acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt
     3541 agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat
     3601 tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa
     3661 gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt
     3721 cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat
     3781 ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt cagaaaacta
     3841 aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa gaaaaaatag
     3901 acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa agatgggaag
     3961 gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg
     4021 gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat cattgctacc
     4081 tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca agtaaaagaa
     4141 agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa tcgcttcaat
     4201 caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat catagcagga
     4261 gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa
     4321 caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc aaatgaacta
     4381 aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg aacattccat
     4441 agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa
     4501 ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt tttgcaacag
     4561 ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac gattgcttct
     4621 cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca gttagttaaa
     4681 gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg actgtatgga
     4741 ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt caatgatgta
     4801 ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat cggccaaaaa
     4861 tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg
     4921 aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga tgatcagagt
     4981 atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa agaatatcct
     5041 accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt ccaaggagca
     5101 aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat tccagcacaa
     5161 aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga aagtcagtgg
     5221 ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga atcaatcata
     5281 ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa aacgaatatt
     5341 ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa gtctttccaa
     5401 catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa gaaaaatcag
     5461 gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa aagtgaatgc
     5521 gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat tttgtcgata
     5581 agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa
     5641 acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct gccacgtata
     5701 gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa gcagcatgct
     5761 actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa agaattaaaa
     5821 acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca gagcattcac
     5881 aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga aggaagtttg
     5941 ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa agcagaacca
     6001 gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag agcaagaaat
     6061 cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt atcgagattt
     6121 atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga
     6181 agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa atttttgtga
     6241 aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg aaaagggagg
     6301 tgtgacaaga attttgtcac acctccctta tttattttg
//
LOCUS       DAJSVD010000132         6322 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSVD010000132 DAJSVD010000000
VERSION     DAJSVD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.135"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            51..389
                     /locus_tag="ON498_002536"
     CDS             51..389
                     /locus_tag="ON498_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940755.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            499..720
                     /locus_tag="ON498_002537"
     CDS             499..720
                     /locus_tag="ON498_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940756.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            740..2896
                     /locus_tag="ON498_002538"
     CDS             740..2896
                     /locus_tag="ON498_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940757.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2908..3141
                     /locus_tag="ON498_002539"
     CDS             2908..3141
                     /locus_tag="ON498_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3940758.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3131..3997
                     /locus_tag="ON498_002540"
     CDS             3131..3997
                     /locus_tag="ON498_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940759.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4012..5982
                     /locus_tag="ON498_002541"
     CDS             4012..5982
                     /locus_tag="ON498_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940760.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            6149..>6322
                     /locus_tag="ON498_002542"
     CDS             6149..>6322
                     /locus_tag="ON498_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940761.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         2432 a          869 c         1238 g         1783 t
ORIGIN      
        1 tgtcgatgat ataattaact tattaaaaat attaaaaagg aggtttttat atgttcgatt
       61 ctattaaaaa tttggttagc ggaatgacgc ctgatatgaa aactttagta caagttgttt
      121 tgtatttctt ggcattcgtt ttttcgttga aaccaggatt cactgcaatg aaattttttg
      181 gtgatgcaaa atggggagat ggattaaaat atgttggggc gattattgca atctttatta
      241 ttcctgtaat tacaaccgtt atgctttaca caatgggtac taaaacaggt aatgatttga
      301 accaaaaagc taatatcata tcagcactta ttccagttgt tggtatctat ctagtttcaa
      361 aatttccaaa aaatgaactg ttaggttaaa agtgaattga acaaatttgg ataatatgcc
      421 gatttgttca atttactttt taccaaaaaa ctacgctcca agcgtagttt agaagtgagg
      481 gaagaaagta aatgtaagat ggtttttgat agagaaaaaa tgttagctca cgccaacgaa
      541 gtattaatga gcagtctgaa aggtaccgaa ctggcaaaga taatgaatat gaatgttaac
      601 cagttctatg attatcgaaa tgggagtaaa aagattgaaa aagccagact tgaaacgctt
      661 attaaattcg aaaaagcata tgtatatatg ctagataaac agaaaagaac tattgactga
      721 aaaaaggagt tttacaataa tggcaaaatt caaagatatt accgaatcta ttaattatgt
      781 ggcagaaaca gttcctgtag ttgcactctc tttgaagcca caattactcg aagtgtacaa
      841 aaaagaagaa aaagaagcga aggcaaaaaa agctaaagca aaggtatgga aagaattcga
      901 tgcgttgctg accaaagatt taccaaagaa aaattatatg gtaattgaag atgtacaaaa
      961 gctcaacagt gagggagtta tagatgagga tgatgctatt ctcgattctg agaagtatgt
     1021 cgttctattg aagtcaagtt tgaattatgt agctgatcta ccagaaaaat ttaaagagaa
     1081 gctattagca ggtgtccgtt ttatggatag tagtggtcaa ttattagtta ttccggatga
     1141 tcctaatgtg tttgaatcag aagttgaaga agctacagac ttctatagcg cagtagagca
     1201 attagaagta gctactaatg gattagaacg aattgctgag accttcaact cttctgaaga
     1261 agttgaattt gaagccgtag cagaaaatgt accagatgaa aaagaagctc tttttgtgga
     1321 tgataacaaa ccaaccgaag cagaaaacca agaagtaagc atagaagaaa cacaaataca
     1381 aacagaaacc taccaacaac caaaaattga tgaaaaaccc gaatcccaaa gtgacaaaga
     1441 aaattatgtt tatgaagctg aaaaaatcac atctgaaagt gatgtaccga aaaacttaga
     1501 tgctgatttt ttagaaaaaa attttgaaca gatcattgct gacaaatttc aacctgtagt
     1561 tttagatgag attaatgacc ctgtcgattt aaacgaggag atgctatcca tacctgagtt
     1621 gaaaccattg ctgtttttag ctaaagagag tatgaacgct cgcatctcta ttgctaataa
     1681 taaaatcaac agtgaattaa ataagacgaa ggagcaattg atcctcaaag gaaagcaaat
     1741 gttagctgat gagcttttgc ggatagagga agcaacctca ttaaatggtc aaaatcagtt
     1801 tacaaataca ctatcggttg ctaaagagag ctacgaagca gaagcatcta aaattgatga
     1861 tttagtgatg gaaaaatcca atatcattcg taaagattat gatatggcga aacaggctta
     1921 tttggatgct gaactaaaga aactagagca acggtatgat gctgagcatt taccagaagt
     1981 taaacaacaa gaagatgaat atagaaaagc aactgaagaa agtcttgatc aacagtacca
     2041 agaagcaatt gaatctatta aaaacgatgc attgaaacaa agccaagaag caaaagaagt
     2101 tgtggtggaa cgaattatca attcattgaa accagaaatt gaaaaatcat taacagacgt
     2161 gcaagaaaaa actttagaga ttgtcgaaaa aacaaagtca gaaaatgaag ctgagttcca
     2221 tgatacaaaa gagaaaacaa cagatgtggt tgtcaaatat tttgcgaaca aagaagaagt
     2281 tgaaaagcag gtagctaaca acacgaaaga attagaaaca aaacttgtag agcaacaaaa
     2341 aactatcgaa gaatataaag atcgaatgaa agaggtcaaa gatttagcag agggttataa
     2401 attaaatagc gaaaagttgg cagaacaaag tgaagcttta agagaagact tatctcgttc
     2461 ccgaagtgat ctagctcaaa tggcagaaaa aacgaaatca ttagcagttg ctacagaaaa
     2521 tcaatcaagt tcaaaacgtg attttacaag taaaaagatg gctactatca tttctgtcgc
     2581 tgcaatggct tgcggaacga tcttgggatc gtcctacttt attagtcata ataatcaaca
     2641 agcacaggct caaactcaaa catctatttc gagagtggaa aattcgattg ggacaaatac
     2701 gaactctgct tcaacaaata gtagctatga agcaacacca actacagagt tcaaagttgg
     2761 gaaagatgtt gtcatcagta ttgatgggaa aacaactaag gctgaagtga tttctattga
     2821 tggagaaaaa gcagttgtga aaactccaga cggaaatcag tacaaaattc aagctcactt
     2881 atctgataat aactaaggag ggctattatg cgagaagaaa aaatagttta tccagtaatt
     2941 caaaacaatc aagaaaacat gacctcttct cagcacttac aggaagcaca gctcgtgtat
     3001 caggtaaatg aactaccaca aacgaacgag acaatgagca agtcaatatc tttaattggc
     3061 ttgcttattg ttgcaatagt gataactgta ggtgtttacc gtaagttgaa cgtgaaagga
     3121 agtatttctg atggcagata actcaaacta tggatttaaa cataacttac atcaaaaacc
     3181 ttctgaaaca attgtcagag ttgaatggct tgaaaagcat gtcaatagca cctattctgt
     3241 tacagcaaaa agattaattg tatcagctat agttgttgca gtcgttgttc ttggatcaat
     3301 tgtttactta gtttctgata cgattaaact gaaatttatt tttgttccaa cgttattaat
     3361 gataattgga tttcttattc taattagcca gtatatgttt attgatccat ctagtggttc
     3421 attgatttct ggttctatta gactgttcaa atcagtagtt aattcagcaa gagtatctat
     3481 ggggaaaaag aaaccatttc gaggcacaga gattttaaaa gtgaaacaag atggaacaat
     3541 cgtatatcga gatgggagtg tagcattaat gttcaatcta gatggggcta catcactaac
     3601 cgcttttcca gaagaagtta tggctcagga atcgaaatca atacaatatc aaaataccag
     3661 agatcgttcc ttagctgaaa tcaaaatcac ttcatctcaa cctcagaata cggaaagaca
     3721 aactcgaaat atgaaaatac aaaaacgaaa tgcaaggagt aacaaagcag tctttgacat
     3781 aatagatcag caagaacgat ttgtatcaga atatattgat ggtgtgaaaa caacagtaat
     3841 ccaatatctt atcttgaaat ctcaatctaa gatggtattg gatgaaggtg tggaacgttt
     3901 agaaaactat acgagtagag gactttatta tagtgtcact cagttgtcag ctaaacaaac
     3961 aaaagaaata ttatctgata tttatgcact gaaatagagg tgagagatta aatggaaatt
     4021 atgggtagat cacaactatt aaatttcaaa aaaaagagaa aaactaaaaa agaacaaaaa
     4081 actagaaatt caaaggtagt gaataatacc tttgttagtg atttaacatc tttagttggt
     4141 tatcaattta caccatctta cattaggaca ggtaagcggt ggggaacaat tgtgaaatta
     4201 gtgaatactt atggaatgaa tagaaattat ggatttggtt ggatggttaa agctattcct
     4261 gaagtgagag tagaaggggt aaaagcctat ctaattgaag cagataagcc agtgtcacaa
     4321 aaagatcaac aagaaataat gcacgagaat gttgggggat caacgaatag ttatgaaaat
     4381 aatttagaag gaaaaaattc agatgctgaa attagcaaaa ctgttatgcg gattatggat
     4441 ttgaatacag catctttact agacagcatg gcaaataaaa ttattgattg gaagctagaa
     4501 atcctattag tatccgattc tcctaataaa atcagcgaac agctaattaa actccaacgg
     4561 ttgtatgatg aaaaaatgta tggattaaag cttatgtcat atggaggaga tcaacgtgat
     4621 ctgtttgata gacttttaag tgaaccaatc gggaatgtct atgagtatac gtctatgagt
     4681 tcggattttg caggaaatga tcatgcaatt cgcaaaggtt tagatgatgc gaatggatgg
     4741 gctatcggca ctatgtctac tagctacgca agtggtgtag cttttatggc actggatgat
     4801 tcttttacaa gtaaagtttt agtggcatca cacaaaaacg ctacaattaa aggctatgat
     4861 gaaaaaatct cagcatcatc actttggggg caattgattg ctaataatgc aatggcaaat
     4921 cataaacgaa cttttcatat tgtcttaaat aaatttgatt actttggtga tccaactggt
     4981 agaacatttg ttgccaaacc ttcgttaaac agtgtaatag aaaaggttaa tttggctgaa
     5041 gaaggtctta actctttgga gctttttggt aatcaaaagg acattggtga tttatataga
     5101 cggaatctcg ataaactaac tcagattttt tatttgttag caggcagaac attagattct
     5161 gcaactagag ttttattgaa aaaagaatta ggtaactttt atataagtca gaaactttgg
     5221 gatattaatt atgaagaacg ttcaaaactt ttggtgaaca gtagtgatac aatgccagtt
     5281 cttggggatt ttattagcta tttgactaat gctgaaacaa aagccaaaaa atctggtact
     5341 gaaaaagaag tcgatcgtat tattgtatta aaagacacat tgagaacagc attagacagc
     5401 ttctctagga tgtttaatca aagaacgaca ctgaaagaaa tcggtaaaag tgacaaatta
     5461 cagttttact ataacttagg tgatctaaga gcagaaacca atgttatgga agctcaattt
     5521 ttaaatacct ttgatttcat cacatcacaa gcaaaaaaag atgatattgt tatgattcac
     5581 ggcttcgata agttatctat tgaaaccgtt gggctaatta aaaaaagatt aacaatgctt
     5641 gaagaagatg gaattcgtct tgcctttcta tttgatacaa tcggttcagg agaagacaat
     5701 gaatacaaag aagatattga aacagctgat atattcaata cagatggaaa aatttaccaa
     5761 agttttgaaa gtgattttaa ttacactatc attggaaaga tgaccagtgg agagttaatg
     5821 aagtatgaaa aaaaagttgc tcctgctaga aaaggagaag atgatcaaaa actttctgtt
     5881 gatttgagac agtatttgac aacaggaaga agtaataaat ttcagatcag gagaccagtg
     5941 gatctgacta ataattttgt tattggagag tttattcttt aagttttatc tttgattttg
     6001 agtgtataag gaaaggataa gaaaaaacat gtatatttga gactaaaaac agtcttagag
     6061 aacatggtac gttttgtgaa aagagaacta tagaaatttc ataaagtgaa accttactga
     6121 gtgaaataat tatagtggag gttaaataat gaaaccaaac ataaaaattt tagatcgaat
     6181 atttctaggt agagacacag aagtaatcct aattcaacat gaagaaggat ttgaagttag
     6241 tataggtatt cagaaacttc aaaaacctca ttattgtaat cagttgtata aaaactttac
     6301 tgatgaagaa aaagcgagag tt
//
LOCUS       DAJSVD010000133         6173 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSVD010000133 DAJSVD010000000
VERSION     DAJSVD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.136"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            725..811
                     /locus_tag="ON498_002543"
     CDS             725..811
                     /locus_tag="ON498_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3940762.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ON498_002544"
     CDS             complement(932..1621)
                     /locus_tag="ON498_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940763.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ON498_002545"
     CDS             complement(1628..1906)
                     /locus_tag="ON498_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940764.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ON498_002546"
     CDS             complement(1866..2780)
                     /locus_tag="ON498_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3940765.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ON498_002547"
     CDS             complement(2762..3142)
                     /locus_tag="ON498_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR3940766.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ON498_002548"
     CDS             complement(3592..3879)
                     /locus_tag="ON498_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3940767.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ON498_002549"
     CDS             complement(3899..4123)
                     /locus_tag="ON498_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR3940768.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ON498_002550"
     CDS             complement(4578..4748)
                     /locus_tag="ON498_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940769.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ON498_002551"
     CDS             complement(4763..5296)
                     /locus_tag="ON498_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3940770.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="ON498_002552"
     CDS             complement(5317..6159)
                     /locus_tag="ON498_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3940771.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAJSVD010000134         5945 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSVD010000134 DAJSVD010000000
VERSION     DAJSVD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.137"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(138..311)
                     /locus_tag="ON498_002553"
     CDS             complement(138..311)
                     /locus_tag="ON498_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940772.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(314..712)
                     /locus_tag="ON498_002554"
     CDS             complement(314..712)
                     /locus_tag="ON498_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940773.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            928..1983
                     /locus_tag="ON498_002555"
     CDS             928..1983
                     /locus_tag="ON498_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HCR3940774.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2003..2356
                     /locus_tag="ON498_002556"
     CDS             2003..2356
                     /locus_tag="ON498_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940775.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2538..2834)
                     /locus_tag="ON498_002557"
     CDS             complement(2538..2834)
                     /locus_tag="ON498_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940776.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2838..3656)
                     /locus_tag="ON498_002558"
     CDS             complement(2838..3656)
                     /locus_tag="ON498_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3940777.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3882..4103
                     /locus_tag="ON498_002559"
     CDS             3882..4103
                     /locus_tag="ON498_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR3940778.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4105..4371
                     /locus_tag="ON498_002560"
     CDS             4105..4371
                     /locus_tag="ON498_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3940779.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4484..5023
                     /locus_tag="ON498_002561"
     CDS             4484..5023
                     /locus_tag="ON498_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940780.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            5517..5801
                     /locus_tag="ON498_002562"
     CDS             5517..5801
                     /locus_tag="ON498_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940781.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
BASE COUNT         2036 a         1030 c          947 g         1932 t
ORIGIN      
        1 tcctttaaaa agcacttatt ttaatataat gttattttat aatcttatta caagctgatt
       61 gttaggcgtt gtcctttcat tacatttatt aacggttatt ttattgttta atttattaat
      121 tatttttgat ctaaaactta gcttaacaaa taaataatta accaaccgcc agataaaaat
      181 gcgatcattg ctaatacata agcaaagata aaaatatgtt cgatcagctt aaaccctgat
      241 gcgattaagc agaccaagta aaatagaaat tctcttacag ataatggtaa ttttctaact
      301 agcatactca taattaacac cccaaagtaa tattgtattt tgaacatttt acatccaata
      361 cagcatcaat caaccattgc caatcaaaaa tacaaggctt cattgaagta tgtttttcag
      421 atgaaaagat ataatataat ttcttcggaa taatattttc agttgttggg atcaagctaa
      481 atctaatacc gtctaactca tagctaccag ctttctctcc attaaaaatt tccttagcac
      541 tttcttctaa agaatcaaaa tatttttcga cagcaatctt tgaagctttt actattgaag
      601 catttccctt ttcgataaaa tctgcttttg ttttaccgac aataagtaat tcttgatcag
      661 taaaatcttc taaaataatg gtagtgtaat tcttatttct tctcaatcgc atcaggatca
      721 ctccttaata taatttatgt ttttataata tcataataag ataaatttgc aagcatttat
      781 ttacaccttt ttatttacac tttattataa ttaattagac ttttatgatt gcattttttt
      841 cttataaaca gttgcaacta atagtaaata ggtgtaaact attttcgtac cgaaatacca
      901 taaacacaaa cagaaagagg tcataccatg aaagaaccat tattgttttc cgttgactta
      961 gggaataaat cagtaaaaga aatgagtaca cacgtttctc cactctcact tccctctcgt
     1021 attttgaaca aaaatatcat taattcagga cgtttttcta atataggttc ctcattaaaa
     1081 cgatcactaa acgtttctga atatagccta gtaaacacta ctgaaagcta ttactttggg
     1141 aaggatatac acgagctagg aaaagatgac tacgtgatag aatcactagg ttttgggatc
     1201 agcagataca agcaggaaac ctaccaaaat atcctatctt tctctttagc taacctagca
     1261 cgtttagaag atcaatctga tgacgtttta accgctaatc tagttttagg tctgccttca
     1321 tccgactata ccgaaacaat cgtcaaatac gtgatgcaac aagccacacg acaacattca
     1381 gttgaagttg acggtaaagc ttataatata aacgtagata ccgtttgggt actgccacag
     1441 ccgattggaa cactatataa cctcctactt gctgatgatg gtagtgtaca agatgaaaca
     1501 ttgatcagtc agaagatcgg ttttgttgat attggcggtg gcacgaaact ttttgatgtt
     1561 gtccaaaatt ttgaaattga cgaaaacaat cgtgaacaaa aggataccgg catttttact
     1621 ttatataatc gtatcgtaaa tatgcttgac cttccgatca gtcctaatgc tttccaactt
     1681 gaaaagatta tccgagaagg caaagaaaaa ggcgtatatc tatttaagcc aagcggaaat
     1741 gaaagttttg atatttcaga agtagttgaa agagaaataa aaacatacac caatatggtt
     1801 gttgcagaag ttttagctac attcaaaaac ttcacaacct tagataaagt tgtctttact
     1861 ggtggtggag cttctattat cgacaaacaa cttgtaaaag ataaaattcc gcataccttg
     1921 tttgttgaag acagtgaatt ttctaatgta aaaggctttt acaagtttgg tttaactcaa
     1981 taaaacagcg aaggagatca acatgaataa gggaaaatca gagcgttatg tagttataat
     2041 caatagagaa aaggttagcg agttaccttt agcagagttt cttgaatccg aatatgaaac
     2101 actaggcgtt aaatactctc agacgatcaa aaaagctttg aacctatatc ggcgtgtaaa
     2161 gaacaatgaa gttacaatta ttgaagcagg caacgataga aacgatagca aagtagcaga
     2221 agtaaagaat aaaccaactg atcataaccc tattcaacaa gagcatcctt caacggttac
     2281 atccgatcat tcagcaaaga aagaaaagcc aatgctgaaa gggttcaatg caccaagtaa
     2341 caaagactta ctttaaaaat aaaaaggcga ggttacaata cctagccttt ttattatgta
     2401 caaatataaa cataaacata tacgtataca tgtatataaa catgtatacg tatatatcca
     2461 taaacacgta tattaaattt agttaagcta tacctaaccg cttattccaa taaaaaaagt
     2521 gatcagcttt aaactgatca ctcaccaaaa acttcatcat acaagttatc tatcatttca
     2581 ccaagtaaag ctgaatctga tcgaattcct aacttttcgc tgattttaga tagtttcttt
     2641 ctttgcgatg gttgaagcgt gaattgataa tttttcttct tttcagaact atcacttttt
     2701 agtttagcta ccaaagaact tgttgtttct ttaggtgttt gtttaggttc ttcaactgct
     2761 tcaaccactt caggaacttc ttctacttca gcttcagcga attttttaaa tctattttct
     2821 ttcacttcaa tcactcctta ttcttgtgcc gtatcaatta ttttgttgta tagcgtaaac
     2881 gtgctttcaa agaaatcctt attcttacga tagatattct tatccttagc gaactcagca
     2941 atattcttac cttctaagct agttgcatta agcaattcct tctcttgtac atatccaaga
     3001 taattagata acgttggtac cagttctagg aactcacgac ttgaattagt attatgtttg
     3061 atcctgctac cgatcgtagc gtatttagtt tccatcagtg tttcaccagt cttaatatca
     3121 atcaactgtt ttgctagtgc atcaatatct ttaccaagct tcagcaaagc ttcaaaacca
     3181 tctttagatg gatcagaaat gcctacgaca agatcagata cagcccaagc attttgagct
     3241 agtagacctg catcattatg agtatcaatc agaattagat catacttctc tttaagctgt
     3301 tcatagttaa caaccatcca tctaaacagt agggttctgt tgttaggcat atcacgcatt
     3361 tgcatctcaa cttgttttaa atcttcacct gctactaaaa tatccatgtt ctcatttaca
     3421 gctaaaggtt cagctgatcc atgaagatat aaatgattga ttgtattctc tcttttcgct
     3481 ttactgaaag catcaggaaa tcgttttgat aggttttgcg acttatcttc atcaatatac
     3541 agcaccttca tacctaaatt ttcgacagca tatccaccta gattgtagat tgttgttgtt
     3601 ttacctgttc cacctttttc aatgtttcca gtaatgattg acaacttctt ttccatgatt
     3661 aaacctccct tattaacctt ttgtaactcc attatacact ataaacacgt ttatgtatat
     3721 gtttatatac ataaacgtgt ttatatttat atttgctttt atattctaca acataaacat
     3781 atatatgtat atgtttatgt ttatgtatat attcatatat ataactattg catttgtcat
     3841 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     3901 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     3961 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gactttgcca
     4021 cctacaagga actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     4081 tgctaaaaga gttaggctta taagatgact taccaagtaa gatatgaacg tgaagctcaa
     4141 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     4201 aatcttgtag gcactgatga tccaagaaaa catggtaaag gtctaacagc taataaatca
     4261 ggctactggc gatatagggt aggtgcctac cgtataatag ctgatattag cgagcatgaa
     4321 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     4381 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     4441 acctaaaaaa gaacaatacc ctcttgttat tatacacact ggtgtgtata atataagtgt
     4501 aaacaagcta attaactttc aaaaggagcg attttttatg ggatggtatg gtgcttattc
     4561 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     4621 agataccaaa gtaacgaaga cctatggtat tatccttttc aaagatgcag aaggaaccta
     4681 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     4741 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     4801 agaaaaaatg gttaagcaac aactagatag aattaaagaa gaaaacaata aaccaaacct
     4861 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     4921 ttatcagtat aaactcaaac gtagctttat ttttagacta caagatggat cactcactcg
     4981 gttcactcga ctatcagcag gagatgttaa ggaagtagcc taaaacttaa atacataaac
     5041 ataaacgtgt atacgtttat gtttatgttt atgtatatgt atatgaatat aaacgtatta
     5101 ggtacgaacg tttttaaatg cttttctggg ctttgatttt agggtagggc taaaacatcc
     5161 attgatccaa aacagctcag aaacgcattt aaacgtatct gataccaata ttacagaagg
     5221 agtgtatcgt atgattaacc tatttctttt agtgacagct agtacattat tctttactgg
     5281 aatatcactg ataatttcct atttgatcta taagaaagcc gaaacttaca caagcaaagc
     5341 aaccggacta gccgtttcct cctttttaac tttgctgact ttttttgtta ctctatcgtt
     5401 ttcttttgtt tagcacagct gatccatcag caaaaaacct ctctttacat tatacacact
     5461 agtgtgtata atgtaattgt aaacaagcta attaactttt aggaaggagt gacaatatgc
     5521 taacagctac attacagcaa cctactcacg ttgctattgc agaaagctat acaattaatg
     5581 caggggtcgt taaacagctg atcaaggatc gagaattcaa aaagtttaac agtcgtcaag
     5641 cgtataacag acagttaaag aaagctattc taaagcacaa aaattccttc atagggaaat
     5701 tggaaagtgt ggatgttaaa actgtttcaa cggcattttc agttgttgaa gtgactattg
     5761 acggtcgctt gatttctcta tttgcttttg gaattaattg atagggagtg tggactttgg
     5821 aaggtaagac ttttgcagaa atttggactg aatttattga ggtaccaact cgcattgatc
     5881 cgataacttc tgaaaaggta ttggatgcag acttctacaa tctcaaagca ggtactagcc
     5941 ttgaa
//
LOCUS       DAJSVD010000135         5689 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSVD010000135 DAJSVD010000000
VERSION     DAJSVD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.138"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(488..1297)
                     /locus_tag="ON498_002563"
     CDS             complement(488..1297)
                     /locus_tag="ON498_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3940782.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ON498_002564"
     CDS             complement(1297..2043)
                     /locus_tag="ON498_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940783.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ON498_002565"
     CDS             complement(2061..2528)
                     /locus_tag="ON498_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940784.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ON498_002566"
     CDS             complement(2528..2938)
                     /locus_tag="ON498_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3940785.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ON498_002567"
     CDS             complement(2949..3962)
                     /locus_tag="ON498_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3940786.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ON498_002568"
     CDS             4179..4913
                     /locus_tag="ON498_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3940787.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ON498_002569"
     CDS             4897..5607
                     /locus_tag="ON498_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3940788.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAJSVD010000136         5667 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSVD010000136 DAJSVD010000000
VERSION     DAJSVD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.139"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(132..1178)
                     /locus_tag="ON498_002570"
     CDS             complement(132..1178)
                     /locus_tag="ON498_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR3940789.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(1189..1461)
                     /locus_tag="ON498_002571"
     CDS             complement(1189..1461)
                     /locus_tag="ON498_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR3940790.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(1496..2404)
                     /locus_tag="ON498_002572"
     CDS             complement(1496..2404)
                     /locus_tag="ON498_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3940791.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(2536..3768)
                     /gene="pepT"
                     /locus_tag="ON498_002573"
     CDS             complement(2536..3768)
                     /gene="pepT"
                     /locus_tag="ON498_002573"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3940792.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(3783..4904)
                     /locus_tag="ON498_002574"
     CDS             complement(3783..4904)
                     /locus_tag="ON498_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR3940793.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(4901..5602)
                     /locus_tag="ON498_002575"
     CDS             complement(4901..5602)
                     /locus_tag="ON498_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR3940794.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         1629 a         1223 c          977 g         1838 t
ORIGIN      
        1 tcgacttata taaatcttaa aaaaacaaaa ttgattgtat gtatataaaa aaagagccaa
       61 acaaacgtaa aattatctta tccataacgt ttttctttga tttgataaaa agggtagatc
      121 ttataaaaga cctacccttg attttcttta ttctcttttt ttacttctat tatttcttct
      181 ttgatcactc ttcgccgttc agcaatcaca tacacaaatc catagagaaa tggcaagata
      241 aaggaactga aacgatacag taaaagcgaa gaaactgcgt caactgttcc aacaactggt
      301 ctgaataata acaaatagac aaattcaaag gaaccaatac cagcaggcgt aggaataacc
      361 cctgaaagca ctactgcaaa gctgataaac gaaatggtca gtagaaaatc tattttagga
      421 tggttttcaa tcagaatcaa ataaggaagt acataccaaa atacaagttt taacatattc
      481 catagataaa tccgcaatag cgcagtcctg tcttggataa tggtttggac cgtttccctt
      541 aaagagtaga tctgctgatt gcatgtatcc acccatttcc gcaaacgctc acttttaaat
      601 aacttattcg ataagctgac aaaaaatatt tgaacattca agctgacgga catcgccaat
      661 aaaaaggcaa caatcacgat cgtcaaaagg ataccagcga tgataaacca gatcattgat
      721 ggaccattag aataaaaaag agagaattgg atgaccaagc cagctaatgc ataagtgatc
      781 actgccagtt tgtacataat catatggagt gctgtcaccc ctgctccctg ggatagcttc
      841 attccttttt tgttataaaa ataaatctcc gagatcagcg ttcctgtacc aaacgaaacg
      901 attcggtaaa acgcaatata gctagaggtc aagaacccat ctttcgttgt aaaattcggc
      961 gcgaaaggtt cagcaatttc tttgattgag cgcccttcag cgatttggta aactgtccct
     1021 aatataacag ccgctgccaa aacaacaaca ctggtagaca gcaattcggc aaagatatct
     1081 gcaaatgatt gattgattaa ataataaata atggcaaaaa tcacaatcag caaaagcaga
     1141 ttcagaatga ttttagtttt cgttccttta gacgacatct cctgctcctt aatggaaaaa
     1201 ataaatgaga atcaaatcaa caagtacacc tgcagcgaat cctatagtaa acttcttttt
     1261 ccgagtttta tgatggaaaa cttttcgaga gatcaatcct cccaaaccac cgcctaacag
     1321 gctgacaaat atcaaattat tctcaggaac acgccagccg ctagttctag cctgatgttt
     1381 atcatacccc atcagacaaa ataagtaaaa attcacaatg aaaaggtaaa accacaaaag
     1441 attgtgtaac aatacatcca ttactcttct tccaactttc tgttaactac tatttctaag
     1501 ctttttcttc tttctttggt gaataaactg ttaatccttt tgatacaaca gataattcaa
     1561 ctggaagatc atctgtcgtg tctccatccg ttcgagtgcc cacagatttt tcctgcgctt
     1621 ggattttcat gcgtttggct gtgaaataag taatgccggg gatcgtatag aatttgcctc
     1681 tcatgattct aggcaaataa aaaataaatc ggaaaatatc taaggaaggt acgatcgtta
     1741 catgaaattt tccatcatct agggtcgctg agtcgtcaaa atttgtaaaa ccgcccacgg
     1801 agttagacat cgtgactgtc aacagttggg attttccttt ccaatgctcc tcgtctgttt
     1861 ctaaatccaa atgatatttt ttcttcttca gcaataactt aaaaaacttc ttgataaaga
     1921 tccacgagcc gtaacgctgt ttttcttctt gactgatcca aactgctgta tctgctaaga
     1981 ttccgattgt cagtgtagag ataatgactt catcattgac ttgaccatag tctattttcc
     2041 gtacattgcc ttccaggatc acgtcagcag cttcttcttt tttgagggga atctcaagaa
     2101 cacgtgcaag gttgttgaca gttccacctg gaagtaatcc gacatggtat tgagataatg
     2161 ttttttggaa cgcttggacg atatgatgaa tcgtgccgtc tccacctata attactaaag
     2221 tatcagcttc tatggcttct gctttcttga ctaattccgc atcatcaatc gatgggccag
     2281 tttcagctag ttctacatat aattctggct gcttttcttt tacataatct tgaaaccagc
     2341 gtgctaattt ctcaccctca tctttccctg aacttttgtt ataaaaaact agcagctttt
     2401 ccattcttta cctcctattt actcttcaaa aaagagggcg ggacaaccaa ttccattgtc
     2461 ctgcccttta gccttagaca gatgacattc catcattggg acgaacgcct tgtcataagc
     2521 tgttcttttt cgattttaac tctttgtgtt caattctgca atttttacaa tcacgtctgt
     2581 tgctttttcc attacttgta aagaaacaaa ttcgaatcgt ccgtgcatat tttctcctcc
     2641 ggcaaataga tttggcgtcg gaatacccat gtaagagatt tttgaaccat ctgttcctcc
     2701 acgaactggt tcgatcactg gagagatacc aagctcgatc attgcttgtt tcgctaattc
     2761 cacgatgctc atatcttttt caatgatttc tttcatgttg taatattgat catacatatc
     2821 tactttgatt cgttcttgat caaatcgctg attcaactct tcttggatat gtgtgatttg
     2881 tgctttgcgt gcttcgaatt tttcacgatc atgatcgcga atgatataac tcatagaagc
     2941 ttcttcagga ttcccatcca atgccatcaa atggaagaaa ccttcgtatc cttccgtttt
     3001 ttctggaact tcatcggctg gaagctgatt atgaaaatcg attgctaact gtagagcatt
     3061 gatcattgta tcttttgccg tccctggatg tacatttttc ccttggatcg taatatttgc
     3121 ttgagcagca ctaaacgttt catactgcaa ctcacctaaa ggaccaccat cgactgtata
     3181 agcaaaatct acatcgaatt gctcaacatc gaatttatcc gctccgacac cgatttcttc
     3241 atcaggacca aaagccactt tgatggtacc gtgagggata gaaggattat tgatcaagat
     3301 ctccattgca gtcatgattt ccgcaatccc tgatttgtca tctgctccca gtaatgtact
     3361 gccatctgtt gtgatcaatg tgtggccttc ataatttttc aaatttggaa aatcttttgt
     3421 attcaatgta tagcgttctt ccgcatctag tacgatcgta gactcgccat catagttttc
     3481 aatgatttgt ggattgactc ctactgcatt aaaatctgct gtatccatgt gggaaataaa
     3541 tccaatggaa cgcacttcat gatcaacatt gcttggtaat gttcccataa cgaatccgtt
     3601 atcttcatta tagaaaacat cagctaagcc taactcttct aattcttttt tcaattcttg
     3661 cgcaaaagca acttgtgtct gagtcgatgg agtcgttttg cttgttgcat cggagcgtgt
     3721 ttctgttttg acatatcgta aaaaacgtgg caaaagattt tcgtacatag tattctctcc
     3781 ttttaattaa attgaaatgg gtttgtattt gtttcagaaa cgaaaaagtt aatatcccaa
     3841 ttttcttctt gtttccaaga ttcaaatttc tcgataaatc gttgcttgca taacgattca
     3901 atataatgac cgggatcaat tgcaatcaac ccagcgcttt gcatatcatg agccgtatga
     3961 taatagatat ctcccgtgat atagacatct gctctttggg cgattgcttg cggataaaat
     4021 ttttcaccgc ttccaccgca aatcgcaatg cgcttcacag aggattttgc attttttggc
     4081 tggactattc tcagtccatc tagttggaac gcttctttta cttgttctac aaatgcctct
     4141 atagaaattt cttgcggcaa ttcacctact cgtcctaacc caaacatttc gactggttca
     4201 tctatagcga aaagatcata agcaggttcc tcataaggat gagctgaccg catggcttga
     4261 atcacttgtt tttcgatagt ttccggcaaa ataacctcaa cttttgcctc ttgaacttgt
     4321 tctgtcttcc ctactttccc aatcgctggt tgtgcaccag cttccggagt aaatcgaccg
     4381 tgtccaatcg aggtaaaact tgtaccggta taatcacctt gtgtaccagc accagctgca
     4441 gctaatactt cacgcatttt ctgtgcatga tccacaggta cataaacggc cagtttttta
     4501 taatgaatct catgagtctt tactaaataa ctctccactt caataccgag aagttcacaa
     4561 aaccagtcat tcagtccatc ccaaataata tccatgttcg tgtgtgctgc atagacggca
     4621 atatcatgtt tcaacaaatc tgcatacatt ttttcttgtg gctgatcagt aaccagccgt
     4681 tttacaggac gaaagattgg aggatgttta gcaatcagca aatcgatttt tttcttgatt
     4741 gcttcttcga ccacttctgg acgaacgtct aatgtcatca ttactcgttg gattggctta
     4801 tccaatgttc cgatatgcag tccgactggg tctccttctt cagctagcca ctgaggacaa
     4861 tagctttcaa acttttggat gaaagtgcgt ccgctgattg tcatggcttt aaaacctcct
     4921 caatcatttg gatttcctgc tcgatttgtt tttgacgtgc ctcttgatta gctgcttgtt
     4981 tgagctgttc acggatagct tttctttgtt gaagctctct tgtccatttt gatttgaaga
     5041 tctcactttt ctcttcaagc agaaatggcc cgaacatcag ctccatttca ttgtaagtaa
     5101 ctggtttttg ttctttttcg gctacaatga tttcatatat ttttttattt tcttcaagta
     5161 ttttttctga ttggatttta tactgatttt cttgcagcca agatcgtaat gtacgctctc
     5221 cgatgtttgg ctgaaggatc aaacgttctt gtccgcttaa gcgttgattc aacttgccag
     5281 cttctaggat atctcggatc agtgaacctc ccatacccgc aatcgtgaca gctgtaatac
     5341 catcttgtgg ctggatagca tctagaccat ttgcaaggcg tacggtgata cggtcttcta
     5401 atccatcttt tttgacttgc ttacgtgctg attcataagg accttgaacg acttcaccag
     5461 caactgcata ttcgattttt cctcttaata tcaatgcaac gggtaagtaa gcgtgatctg
     5521 atccaatgtc tgctaaacga gcggattcag ggacaaagtc acccactgcg gctaagcgct
     5581 gagataaatc taaatggttc atagcttcac tccttctaga taattatagc attttccaaa
     5641 taaaataagg agactaggaa taaaaaa
//
LOCUS       DAJSVD010000137         5646 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSVD010000137 DAJSVD010000000
VERSION     DAJSVD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.140"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(680..973)
                     /locus_tag="ON498_002576"
     CDS             complement(680..973)
                     /locus_tag="ON498_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR3940795.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1376..2554)
                     /locus_tag="ON498_002577"
     CDS             complement(1376..2554)
                     /locus_tag="ON498_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3940796.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2658..2972)
                     /locus_tag="ON498_002578"
     CDS             complement(2658..2972)
                     /locus_tag="ON498_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940797.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3079..3294
                     /locus_tag="ON498_002579"
     CDS             3079..3294
                     /locus_tag="ON498_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940798.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3594..3836)
                     /locus_tag="ON498_002580"
     CDS             complement(3594..3836)
                     /locus_tag="ON498_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940799.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3823..4251)
                     /locus_tag="ON498_002581"
     CDS             complement(3823..4251)
                     /locus_tag="ON498_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3940800.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            complement(4403..>5646)
                     /locus_tag="ON498_002582"
     CDS             complement(4403..>5646)
                     /locus_tag="ON498_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3940801.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT         1739 a         1112 c          889 g         1906 t
ORIGIN      
        1 aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta attgaagtat
       61 tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat tatctactat
      121 ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg tcgactgcag
      181 tgttgtgtcc ctcatcacga ataaacggtg ttcaactagc tgatcttgta ttcacttaaa
      241 aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc aggacaaaat
      301 actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa cgaaatcttg
      361 ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt ttggtattta
      421 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
      481 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
      541 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
      601 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
      661 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
      721 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
      781 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
      841 caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa cttggtactt
      901 gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct tgctccgtat
      961 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
     1021 ttctggattt tttattaaag cggttactct taagaaagat attatcatag tgctcttttt
     1081 tttactacta cttataggta gatttattct aatccattat tctcccctac caataattaa
     1141 agaaaaaact ggatcagggt gttcaaatta catgctaaga aaaaagaacc cttgaatacc
     1201 aagggttctt tctcagtctg ataacaatga ttaacgacgg atttctttga tacttgtgaa
     1261 aagtcgttaa agtattgata taaaaggatt tttattacct ctgactacat attgactaat
     1321 ttgttaaaaa atcgtttttc aagaatcctg taataccagt gctttcaccg tttttttaac
     1381 tctctaaaga actaaaagca atcagaggtt ttaccttttc atttgacgtt aagtggcgat
     1441 aaattttcga agttgtttgg tcagtatggc ctaagcgatc tttcaagact tcccagctac
     1501 agtgttccgc tactaagctt gccattgtat gacgagctaa atgagtatga aatggcttat
     1561 taattcctga gcgattcacg caccgtttga cgtgatcatt tacatactct cgacgataag
     1621 gaacaccacg tttactagaa tcaagataca caaacaaata tgtttcttct ataataaacc
     1681 tgtaatgttg cctcatctca cccaaagcct taatcaactg ttgtagcttt tccataacta
     1741 gaggagttac tagaactcgt ctagcagaac taactgtctt agtagtttca attcgaaaat
     1801 ccttactctt acctttaact gccgtttgct tatagaacag aatttcattt cttttgaaat
     1861 ctatatctcc aggctgtaga gcaagcgcct cgctgactcg gcagccagtg taaaatagaa
     1921 catggaaaag tacagacatc ggtaattcct catgaacaat gccataattc aaaaaagttc
     1981 tcaattcatc gacagtaaga aacttgtcct caatattatt tatggcttct tttaaatcag
     2041 ttaccgttaa ctgatatttt ggcattctca cagacttcat tggattttca cttatcaaat
     2101 tctttctttg acaatattta aaaatcatat ttagaacttg aataagtgat tgtaaactat
     2161 ttttggcata gccaacattt gttccattaa cgccacattc ctttaaggta aaaagcaatt
     2221 cttgaaggca ctctggtgtc aaatcagaca actttttatc accaatccat cgaacaataa
     2281 tttttaattg ttcctctcgc ttatcatagg ttatttcctt ttgttcattt aacgaacggt
     2341 aatattggaa ccattgctca cctacaacac taaacttgtc attgcttgca acaatttttt
     2401 tcttaagcct ttgttgtttc tccatttcca gataagcgag ttgcgcctca cgttttgaat
     2461 taaagccacg tcttgttgtt cgatatgctt gaaagtacca tgtgatccta ccagtagaac
     2521 tgatttgctt cttgaatcga tctgtttttt ccatttattg attcctccta ttttgaagga
     2581 acccaacaag cagaaaccct ttggaaatct ttctatctat gtcatcattg taccgcatct
     2641 tagaaagaaa atgtaactta taaagatcga agtagtcgca atttttctcg atagtcattc
     2701 gaatcactct ttctcatcca ttctaaaaca tcatcacgaa aatagtgtcc tcgaatcccg
     2761 taccaacagg gaggaaactc accaagtttt actaaacggt tcagtgtact atcacttatc
     2821 tgaaatagtc gctttaaatc agtatttgtt aataccagag gtaaatgact gactgcaagc
     2881 aatttttccg tgtaatcttc cagttttttt tgtaaaattg aatagaactc tatttccaga
     2941 gaattaacat ttgttttaac ttccaactcc atcacccttc tctctcaaaa tcaaaacgac
     3001 caccacggta ccccttaaaa ttgtaaaaaa acactccagt actccatcaa aacggactag
     3061 aaaagaggaa tttttaatat gaaacaagga attgcagaca taaaaataat taaagaaatt
     3121 cttgaaaaat ctacggctaa tgcaatagca ttcggaacag gaatcaactt gagtacagta
     3181 aaaaaactta aaagtggaga gagagctgaa gaaaaattaa atcttgccga tgctattaaa
     3241 atcacagaat ttggcatgaa aaatatgcca accaaaatcg aaatatggaa atagtagttt
     3301 ttctgatcta tcagctgttt tgccaaagca cataggaatc tcacctcccc ccagtaatgg
     3361 cgggccgatt tggttacgga agtatcatta tcgtagtcgc gattctcata ttaaaatttg
     3421 tcgcttaaaa gctaaccttg tcatggcgta actagagaat caaaatctac atgtactgat
     3481 agatttgcta ttcatttgtc aaagagcgct aggctatttt cagcctatgg aaatccttta
     3541 ttttgacccg ataaaaaact tccatagaat gaaaaggaaa gggaaaaaat actttaacga
     3601 gttgtatcaa attcaagtat cttctcaatc aattttgttt ccagttgtct acgcatatat
     3661 tcatctacat acataaccat tcctttttct tcttcataaa ctggtttcat ggctaaccgt
     3721 gaaatataat tttgatattt tgacagaacg atggacatgg caattgaatc accagaagct
     3781 gcggctttga ttgtcgttag tggtatgaat ggtatatatc gtttattttg cttcatattt
     3841 gacacccaaa tactttccta gctcttccaa agccatttgt cgttggtacc aaacactgcc
     3901 gagacttttc ccaataatat cgccaatttc acgatcatta agaccgatga agaaggacat
     3961 aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca actcctcatt
     4021 ggaaataaag attttcatac caaggacaat aaaagtacca ctactattca aatttggttc
     4081 caaataatag aactctgtac ctaacccttt ttgttccaat atttccattg atatttgccg
     4141 ttggcgaaat ctagcgtact tcttttgaat atttaacgcc tcattgcaga ctactttctt
     4201 acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca taatattctc
     4261 cccctcaaaa aattcattca actcgtttta cttctatccc tttccattat tactacaatc
     4321 taggaggtaa gcgccccata gaacagtacc tgtacgtcaa ataacccgac ctatagaata
     4381 gaatcgggaa tgtttggcgt ttttagttac gagaaaaatg ttgttgtaca tccttgttcc
     4441 aaggcaacag ttgatctaaa gtttctgctg aaagtacggg aagatttggt atcttatcaa
     4501 atagaaactc tagatacttt acaggattca atccgttaag ttctgctgtt ttatatacac
     4561 tcagaatgat tcctgaagtt cgtgctcctt caagacttgt ggaatgcaac caatttttcc
     4621 gtcctatcac cagctctttg atatttcgct ccgcacgatt atttgtgagt tctaaacgac
     4681 catcttcaaa aataggttca aaaccacttc gtagtttaca cgcatattga acggctgcat
     4741 ccaacttgga ttttgcaaca ggatctatcg ttgccatcca ctcataaaaa gcaataaata
     4801 atggacgcag ttcttgttgc cgacagctta atcgcttctc aggagaaaag tctttccatt
     4861 tcttttctag gacaaaccat ttatccaatt gtttgactgc ttgagctgcg ggaatatccg
     4921 tgtttttctt tccttttgga attgcttcga aaaatttccg ccgaacatgc gcgaaacaat
     4981 agacaagtcg tacactttcc acggcattat acccgctata gccgtcacag tgtaaatacc
     5041 ctgtaaactc cttgagaaaa gttttcggaa catctccagc acgactttcg tcgtggtgat
     5101 aataaacgat gggatgctga ctgtcttttc ctgtacaaaa ctgccagtaa taggtatctg
     5161 ttttggcact ttcaatcaca cgataacttg tttcatcccc gtgtagaatc tcttcttgat
     5221 taagatagac tcttaatcgt tctcctaaag ggtctaacgc gttttgcact gctttaatat
     5281 gccaattggt gatattatct ctcgaaatat ccaacccatg tgtttgtttc caatagttct
     5341 cttgccgata ggcaggaact ttctgaccaa atttcatccg gatcgtttcc gccactaaac
     5401 tattggaacc taagctatta gaaatcaaag gtttaggaac gatcgctttt ttaaaagaag
     5461 aaagtccttc tttttcgcag cttttacaac aataggtatg ttgataatgg gtgagacaat
     5521 ccaacatagc ctgatggaaa cgaatttctt ctcgtacttt tgtccgtccg atatctttca
     5581 tctgtgaacc gcaatgttca caaaattgtt cttccaaagg aaggagatgt tctttctctg
     5641 ttatag
//
LOCUS       DAJSVD010000138         5628 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSVD010000138 DAJSVD010000000
VERSION     DAJSVD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.141"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(287..790)
                     /locus_tag="ON498_002583"
     CDS             complement(287..790)
                     /locus_tag="ON498_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3940802.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1149..2174
                     /locus_tag="ON498_002584"
     CDS             1149..2174
                     /locus_tag="ON498_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3940803.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2497..3375
                     /locus_tag="ON498_002585"
     CDS             2497..3375
                     /locus_tag="ON498_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR3940804.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3851..3967
                     /locus_tag="ON498_002586"
     CDS             3851..3967
                     /locus_tag="ON498_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3940805.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4895..5233
                     /locus_tag="ON498_002587"
     CDS             4895..5233
                     /locus_tag="ON498_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940806.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5327..5557
                     /locus_tag="ON498_002588"
     CDS             5327..5557
                     /locus_tag="ON498_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940807.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1897 a          846 c         1043 g         1842 t
ORIGIN      
        1 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
       61 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
      121 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
      181 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
      241 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
      301 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
      361 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
      421 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
      481 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
      541 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
      601 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
      661 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
      721 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
      781 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
      841 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
      901 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
      961 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
     1021 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
     1081 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
     1141 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
     1201 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
     1261 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
     1321 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
     1381 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
     1441 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
     1501 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
     1561 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
     1621 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
     1681 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
     1741 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
     1801 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
     1861 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
     1921 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
     1981 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
     2041 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
     2101 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
     2161 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
     2221 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
     2281 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
     2341 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
     2401 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
     2461 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
     2521 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
     2581 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
     2641 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
     2701 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
     2761 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
     2821 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
     2881 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
     2941 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
     3001 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
     3061 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
     3121 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
     3181 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
     3241 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
     3301 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
     3361 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
     3421 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
     3481 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
     3541 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
     3601 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
     3661 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
     3721 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
     3781 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
     3841 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
     3901 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
     3961 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
     4021 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
     4081 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
     4141 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
     4201 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
     4261 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
     4321 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
     4381 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
     4441 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
     4501 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
     4561 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
     4621 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
     4681 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
     4741 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
     4801 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
     4861 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
     4921 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
     4981 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
     5041 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
     5101 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
     5161 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
     5221 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
     5281 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
     5341 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
     5401 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
     5461 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
     5521 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
     5581 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatat
//
LOCUS       DAJSVD010000139         5594 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSVD010000139 DAJSVD010000000
VERSION     DAJSVD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.142"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(60..611)
                     /gene="raiA"
                     /locus_tag="ON498_002589"
     CDS             complement(60..611)
                     /gene="raiA"
                     /locus_tag="ON498_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3940808.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(739..1458)
                     /locus_tag="ON498_002590"
     CDS             complement(739..1458)
                     /locus_tag="ON498_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR3940809.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1455..2768)
                     /locus_tag="ON498_002591"
     CDS             complement(1455..2768)
                     /locus_tag="ON498_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3940810.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2849..3169)
                     /locus_tag="ON498_002592"
     CDS             complement(2849..3169)
                     /locus_tag="ON498_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940811.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3277..3564)
                     /locus_tag="ON498_002593"
     CDS             complement(3277..3564)
                     /locus_tag="ON498_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940812.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3691..4335
                     /locus_tag="ON498_002594"
     CDS             3691..4335
                     /locus_tag="ON498_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR3940813.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(4394..5101)
                     /locus_tag="ON498_002595"
     CDS             complement(4394..5101)
                     /locus_tag="ON498_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3940814.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5321..5491)
                     /locus_tag="ON498_002596"
     CDS             complement(5321..5491)
                     /locus_tag="ON498_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940815.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         1612 a         1176 c          967 g         1839 t
ORIGIN      
        1 aacttgcggc agctgtcagt agctgctgca agttttcgtt gttatttaag taaggctgat
       61 cagtctgttt caatcagtcc gtattttcca tctttacggc ggtaaacgat acttgtacca
      121 tttgtttctg catcttcaaa aataaagaag ttatgtccca gcatgttcat ttgtaagact
      181 gcttcttcac tatccattgg tttcaatgaa aggcgtttcg tacgtacgat atctaattca
      241 gaatcatttt cgttttcgtc ctcttcattg aataaagcag cagcatctgc agtattcatg
      301 cccgtttcac gggatttacg attaattttt gttttgaatt tgcggatttg gcgttccaat
      361 ttatcaacaa ccaaatcgat acttgcgtat aaatctggag aagtttcttc ggcacgtaat
      421 acaagataag gtaatgggat cgttacttcc acttttgctg ttttttctgt ataaactttt
      481 aagttcacat gcgccgttgc atcgggtgcg tcactgaaat agcgttctag tttgcctact
      541 tttttctcaa cgtagtcgcg aatcgcttca gttacttcga tattttcgcc gcgtacatta
      601 tatctaaaca taaaaattac ccctttcgtc taccacaatc aagaaggaga aggaccactc
      661 tttccctctt cttaattcta ttatatcgaa ctctaggtta tttgcaaatt aaatcgctat
      721 caaatttcca catctaattc atctcgccaa agaaatggtt cggatgattt tcggctgtgc
      781 gacctgcaaa caatctgctg cgtggaaaag agtctgtcct gttgtataga catcatcaat
      841 aatcaatatt tttttatttt gtatcttaga aaaggtgact gcaagttcaa aaggttgagg
      901 catctccaat ctttcctttc gcgtgttttt tgcttgagcc agcagatgct tctctctttt
      961 gagtagcatc tgaaaaggaa ttcttgctgc tttcaacatc tcgcttactt gattgaatcc
     1021 tctttctgtt cgcctttttt cagataaagg tattggacaa tagatgtatg tcgggtattc
     1081 tttttttatc tgcgtccatt tatatgcaaa ggtgcctgct agtcggatat ctcccataaa
     1141 cttgtattga ttgagccagg ccttcatatt ttcatcgtac acaaaaaagg cacggtgctg
     1201 aaaatcatag gtcggatatt tcttcttcca ttcttgacag tcactgcagt aaacgggaat
     1261 agactttact ttctttttcg tactctctag attgaaatgg attggttttt gacagccaga
     1321 acagcctctt gtttccaata aatgaaattg tgcgcgacaa actgaacaag tctgaggtaa
     1381 tctgtatttc gaaacaccaa taatctcgct gaatgttaaa ttttggaaga tatctttact
     1441 gcagcaactg cacctcacag catcttcttc ccttcttcat tcatttcttt gatttcctga
     1501 caagcttttc gaatagctgc agttttttct tcatagaaaa agtatacttg actgttcgta
     1561 taaggccctt tacgatcggc tcttcctgcg atttgtacca aagaagattt agtaaaaacc
     1621 ggatgatccg ctcctaaaac gataacggat attctttcaa atgtgactcc acgttcaagg
     1681 atcatcgtgg ttaacagtat atcatatttt gcttctcgca ttttctggac tttttccgcc
     1741 cgctgattgt ctcttgaaga aacatctgtg atctcatatc tcggaagagc ctcttgaagt
     1801 gtggctttca aacgatgcat catcgtgata cttggacaaa ataaaaggat atggttttct
     1861 ttaattaatt gatgaatgat ccgcaccaaa gattttatct tcttcttgtt cttgcatttt
     1921 tgtcgccaat tattcatgaa aaccaagacg ggttcaggaa gcaaacgccg atgaaagcgt
     1981 aaagcaagtt tgtggatgtc aaacgtctgc ttgatttcct ttagtaactt tttatcaggt
     2041 gtggcactta agtagatgaa tcgtccgtct ggtttaatcg ctgtttgcac agcatgagct
     2101 aatcctgcat ccccgttata aggaaacgca tcgatctcat cgatcactat taaatcaaag
     2161 gaccgataaa aacggtatag ctgatgaatc gtacagataa caaaagaagt aaaggtataa
     2221 gccgttgctg tattcccatg gagcaacgtg attttttctt ctggaaaaac ataacgatag
     2281 cgtaaaaaca attcattaca aacatcgact cgcggtgtac aaatcgctac tcttcctcca
     2341 gcttttaaag tttccactaa aataggataa agcatctctg ttttccctgc acctgttaca
     2401 gcccaaataa gatgatgtct tttttctgaa tggcaaagta ttctctttga gatttccgtt
     2461 tgtttttcag taagctgtcc tgtccaaaaa aagacgactt ctcgaggttt tgcttcaggc
     2521 tgatcaaaca aatataaatc ttgttgagtg tcgcatcgcc ctaaatatag acaatgtgta
     2581 caaaaataac cgtaatctgc aagctgtcca tcaatgacgg acgtcctcat tccacagcga
     2641 tgacaaagaa tccagttgcc caaacgttcc actgctgcga tcttctcata atttggtaaa
     2701 tttacctcct cacagtctat tgcttcaata tctctcgtca gaagccttct accccaaaaa
     2761 tgattcatat tcttcgccct aaaaaaatat acgaaaaaac aagcgatcta ttttttggat
     2821 cgcttgttcg atagttatac tgttctattt attcacctat gagcgtgatt gcttgatcta
     2881 acactttttc gccattcatc ataccataat cagccatatt gatgacatct aaaggaatgc
     2941 cttttggttc cagacgtttc tcaaaatcag ctttcataaa gcgaacttgt gggcctaata
     3001 acaacacatc cacgtcttta tttgcaaggt tgttatctgc gtcagaagcg gacacagcga
     3061 aaatatccga gtcgattcct tttgcttcag cagctttttg catttttgtt actagtaaac
     3121 ttgtgctcat accagccgaa catacaagca tgattgtttt tttagccata cttttctctc
     3181 ctttttaaaa caaactatgc aaacgttttc ttcgcgtctg ttactctatt atataaaaag
     3241 aaagaaattt gtcaacgttt tctaaacgcg gtttatttaa tcaaatagcc aactagattt
     3301 cactaattta ttgaacacga catattcttt gtccaaattg atgacaacta cttcatagcc
     3361 tgcatttatc cagccatagg cacctttttc agggtgttta taatcatttg cccaccaatc
     3421 tgtactttcc cttgctgttt tgggaagtcc gttactggga aacagcagtt cgtcaaattg
     3481 ggtaaaggtc aacgttactt gagagccgcc attgttcttc aaataatgcg taattgcatc
     3541 gtactttttc attcgttcct gcatccccag atccacctgc ctttgtcttt attatcgcat
     3601 actacagcaa ttgcgcaacc gttacagtgt tgaaacaacc tagaacttta tttatactga
     3661 ataaagaaaa tgaaaaaaag aggtgacaag atgatcgagc gctatcgtac aataaaagaa
     3721 aatggacaaa gtgagattga gatcaaaaaa tcacgcttta tctgttctct tagacgaata
     3781 gaatcagagg accaagcaaa aagtttcatt caagaaataa aaaaagaaca ttggaaagcg
     3841 aaccataact gcagcgcctt catcattgga gaaaaaaatg aaatccagcg aagcagcgat
     3901 gatggagaac caagtggaac ggcgggcgta ccgatgttgg aagtcctcaa aaaaaatcaa
     3961 ctgatcaatg tagctgcagt agtcactcga tattttggag gaacgaaact cggcacaggc
     4021 ggattaatac gagcatacac tcatgcggtc tctcaagcat tgtctgacat tggaatcgta
     4081 gaaggtacct tgcaacagga gatctctgtg atgatcacgt atccgcaact aggaaaatta
     4141 cagaattatc ttgaactcca acattttttt ataaaagaaa tccaatatac tgatcaagtt
     4201 agcgtcatct gtgctgtaga tgaaaaaagt gctgaccact ttaaagaatc catcattgag
     4261 ctgctgaacg gacaagtcca atttgaagag ggttctgttt cttatcacga gcaactgata
     4321 actgatcata tataaaaaca ggtgattctg attctctttt atttctaata aggaatcagg
     4381 gatcacctgt tttttatttt tccaaataaa actcaaaacg atttcctacg tattgcgtac
     4441 gcacatactc aaacggcgta ccgtcttcaa aataagaaat ctggcgcaaa cgaagaactg
     4501 catctcctcg ttttacctct aaatagtcag caattttctc agaagccaac acagcagtaa
     4561 tcgtttgatt cgctggaccg atctttttcc cgcctttttc ttctagcgtg cgatacaacg
     4621 attcagtaat ctctgctttg ctgtagccat cgatcagctt ttgtggaaca ctggcaactt
     4681 caaaacaaat agggacatca tctgcgaaac gaatacgttc cattcgcaag atcgtgtcat
     4741 ctttactcaa gtttagtttt tccatttcac ttgagcttgg cgaagtcaag aaataagaga
     4801 ttgtccgact agacggtact cgattttgtg attccataat ttctgtaaaa cttgttgcac
     4861 cagacatttt ttcttgtact ttttctcgcg caacatacgt acccgaaccg atctttcttt
     4921 ctaagattcc ttcatctgcc aagttttgaa ttgcttgtct caacgtcata cggctgacat
     4981 tgaatttcac tgctaattct ctttctgaag gaagacggtc tccgactttc catataccct
     5041 gttcgatttc attttttaat tggtcatgaa tctgtatata tacaggtaat tgattcgaca
     5101 taacgtacac ccgtcctttc gtttcttcta ttataactgg tatatacctg attcacaact
     5161 gaaaaagata aaggtttcta tccatcaaca gaggatttag acacacatcg ttctccctac
     5221 tcctattgtc agaaacaatt ttttagatat gttcaaccta gaaaataaca aaagacccta
     5281 aaatcgatga aaatgccgac ttatggatct tttgttatta ttagcggttt ttagccatcc
     5341 atttttttgt atgattcgtt gggcgttttg attttacctg gaccgctttt ccgccttttt
     5401 tcggaatatt cacttcaata ttgtattttt ctgatttgac caaatcatat aacttttcag
     5461 ctgcttcgtc tactttttct ttccatttca tgtggatccc tccaattctt gtagttaact
     5521 taactctatc ttaattcagc agaatcttca aggaacaagg tagcaaagca aaaaaaagag
     5581 ctctcgaact aacg
//
LOCUS       DAJSVD010000140         5415 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSVD010000140 DAJSVD010000000
VERSION     DAJSVD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.143"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            68..1243
                     /gene="lacG"
                     /locus_tag="ON498_002597"
                     /pseudo
     CDS             68..1243
                     /gene="lacG"
                     /locus_tag="ON498_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
     gene            1266..1589
                     /locus_tag="ON498_002598"
     CDS             1266..1589
                     /locus_tag="ON498_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3940816.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            1600..3282
                     /locus_tag="ON498_002599"
     CDS             1600..3282
                     /locus_tag="ON498_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3940817.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            3488..4446
                     /locus_tag="ON498_002600"
                     /pseudo
     CDS             3488..4446
                     /locus_tag="ON498_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4741..5100)
                     /locus_tag="ON498_002601"
                     /pseudo
     CDS             complement(4741..5100)
                     /locus_tag="ON498_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1733 a          851 c         1129 g         1702 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaacccatgg agccgtattt ttccaaaagg ctatggggaa gtgaaccaaa caggagtgga
      121 atattatcat aaggtatttg ctgaattgaa taagcatggt attattcctt ttgtaactct
      181 tcatcatttt gataccccta aagaactatt tgataatgga gattttttaa atcgagaaaa
      241 tgttgaacat ttcgtaaact atgcacagtt ctgtttcgat gaattttctg aggttcatta
      301 ttggagtaca tttaatgaga tttatccagt tgcttcaaat caatatttga tcggatcgtt
      361 tcctccagaa gttcaatttg atatagagaa gactgtaact tgtttacaca acatgatgta
      421 tgcgcacgca aagacagtaa acatttttaa agatggtgga tataaagggg aaattggtgt
      481 tgttcactcg ctagagacaa aatatccagc aagtgacaaa gaagaagata agcatgctgc
      541 gaaattagat gatgcgttat caattaagtt cttattagat gctacttatt tagggtatta
      601 ctcagatgaa acaatggcac atattaatga gattttagca gcaaatgggc aatctgcaga
      661 tattgattct aatgatttta gagagatgaa aaaagcagca aatcgaaatg attttcttgg
      721 tatcaataac tatggtagcc attgggttaa agagtacaaa ggagaaagca cacttcacta
      781 caacactacc ggagaaaaag gtagccaagt ctattgcatt aaagggattg gcgagagaat
      841 cgtgcgtgag gatcttccga aaaccgattg ggattggatc atttatccaa aagggttata
      901 tgatttactt gtacgaatcc aagacgaata tccaaattat aataaaatat acattactga
      961 aaacgggcta gggtataagg ataattttga agatgggatt attctagatg atgatcgaat
     1021 cgattattta cgccagcatt ttaatgcgat ttccgatgct atcaaaatgg gagttaatgt
     1081 aaaggggtat ttcatttggt cattaatgga tgttttctct tgggttaatg gatacactaa
     1141 gcgttatgga ctattctatg tagatttcga tacacaaaaa agatatccga aagagtctgc
     1201 atattggtat aaatttgtta gcgaaacaaa aacgataata taaaaatagt taggagggca
     1261 aggaaatgga taggaaagaa ttgcaattat taggatttga aattgttgct tatgcaggag
     1321 atgccagaag ttcattatta acagtgttga aggaagtgag agaaggtaat tttgataatg
     1381 ttgaacgatt attagcggat gctgatgaaa atttaacctt agcgcataac tcacaaacga
     1441 aatgtttagc tgaagaagcg tcagggaacg aaatggaagt aggcgtaatt tttattcatg
     1501 ggcaagacca tttaatgaca acgattctat taagagaact tatcaatgat tttgttgaat
     1561 tgtacaaaga aaaatttgct aaagcatagg agaagaaaaa tgaatacgat tattaagcaa
     1621 attgagaagg gaaaaccgtt ttttgaaaaa atctcacgta atatttattt aggggcaatt
     1681 cgcgatggct ttttaactgc tatgccagct attctatttt caagtatttt tattatgatt
     1741 gcggctgttc cagaagtatt taacgtgacg ttaccagagg cattctcgac gtggctttgg
     1801 aaagtttatg gttattcaat gggattagtt ggtcttttgg ttgttgctac aacagctcga
     1861 tgtttagcta attcaatgaa ccgtcaatta tctgattcag gtaagcagat taatgaagtt
     1921 tctgttatgt tagcgtcaat ttgtggtttt gtaatgatgg cagctgatca aattgatggt
     1981 gggatagcta ctgattattt agggacaaag ggattattag cagctttcgt ttctgcgttt
     2041 gttactgtaa atgtgtataa attctgtgtt aaaagagatt tgacgatccg tatgccaaaa
     2101 gaagttcccg gaacaatcac acagatgttt agagatatta tccccttttc aattgcagta
     2161 tttattgccg ttttgattga tacgggagtt cgtcaatttt ttggaacatc atttgcagca
     2221 ggtgtaattc aattattgca gccattattt agtgctgctg atggatatgt cggtattgca
     2281 atcatttggg gagccatggc aatgttctgg tttgttggtg ttcatggacc atcgattgtt
     2341 gaaccagcga ttgctgcaat tatttatgcg aacgtagaaa ctaactttga attatttaaa
     2401 aacggtgcac aagcctcaaa agtattaaca gttggtctag gaaactttgt tggaacgatg
     2461 ggtggtactg gagcgacact agttgttcct tttctgttca tgttgttcgc taaatcaaaa
     2521 caattgaaag cagttggtaa agcttcattt attccagttt cgtttgcggt aaatgaaccg
     2581 ttactatttg ctactcctat tattttgaat ccgtactttc taattccatt tttattagca
     2641 ccaatcgcta acgtatggat attcaaactt tttgtcgatg tcttggggat gaatagcttt
     2701 atatccgtat tgccttgggc gacgcccgca ccgattggct tggttttagg aaccgggttt
     2761 agtgtgttgg cgattgtatt agcggtagta ttgattgttg tcgatgcgat tatttattta
     2821 ccttttgtta aggcctatga ttctgaatta gtagaacaag agttaaaaaa cattgctgca
     2881 gaagaagtaa aagaagaatt ggtcgaagaa atccaagaag ttggtgcaac tgcggcagtt
     2941 atagttgatg aagcaaaagc atcggttggt gaaggaaagc gcgttttagt actttgtgct
     3001 ggtgccggaa cgagtgcaat gttggctaat gcattagaag agggcgcaaa agagacaggt
     3061 gttaatcttt ctgctaaagc aggagcttat ggttctcatt atgatatgat gaaaaatttt
     3121 gatttaatcg ttctagcacc tcaagtgaat tcctatttgg atgatctccg taaagatgca
     3181 gacaaattag ggataaaagt aattgcaact aaaggtgcag aatatatcaa tttaacacgt
     3241 gatcctaaag gagcagttga ttttgtattg aagcaatttt agagcttgct tagattaata
     3301 aagcgctagc caacttagct agcgttttat cttaaacaca atggtaagcg ctatcttttt
     3361 ttgctgttta aatttcgggt attctttttg aggcgtcgat tgtaaaatta agctagacaa
     3421 ctaaaaagat catttgtgct acactcaaaa tagttccgac caaagaatta ataaggagtg
     3481 agtacaaatg acctacaccc atcttacaac agacgaacta gtaatcatag agtcttattt
     3541 caatatcgag aaacctgtcg ctcttgtcgc acaaagttta aatcgctcta gacaaaccat
     3601 ttataatgtt tattcgtttc tcaaacaagg aaaaaagtgc tagattatta ccgtcaatac
     3661 aaacaaaata aatctcgttg tggacgacgt tcaaccgttt tatctgatga acaatcaaca
     3721 tatgttcaag aaaaggtagc tcaagggtgg tcacccgatg tgattatcgg ccgtggcgag
     3781 tttccaattg attgttctgt tcgtacactc tatcggatgt ttaaaaaagg cctttttgac
     3841 acacagtcgt tacctatgaa ggggaaacgt aaaccgaata atcatcaaga aaagcgtgga
     3901 aaacaggcct ttcgcagaac gattcatgaa cggaacagtc tctatccaca cctgactaaa
     3961 gaatttggtc atctcgaagg cgacaccatt gtgggagtta aacacaagag cgctgttatt
     4021 acattggttg agtgtttatc caaggttatt attactttga aaccgacagg cagacgagcc
     4081 gaagatatcg aaaacaggtt aaatgattgg ttcaaatcca tgcctaaaca cttgtttaaa
     4141 tccatgacat ttgattgtgg aaaagaattt tccaactggc gatctataag taactcgaac
     4201 gatattgcca tctttttcgc agatccagga acaccttcgc aaagagggct aaatgagcac
     4261 tcaaacggct tgcttagacg agatggctta cctaaaacaa tggactttag ggacatggat
     4321 gaaaccttta ttcaatccgt tgcctcgaaa agaaaccata ttccaagaaa atcattaaat
     4381 tatcgaacac cattggaagt atttttgagc tgcatagata aagacatatt gtctagctta
     4441 atttgacaaa tcagatgtct ataaaattcg ttagaaaata caatttatag atatccaatt
     4501 gatttgtaaa caaaaaggag ttgtcaacta agccactcct ttttgttgta atttttccca
     4561 ataagaaagt ctactacttt aggaagataa tagtaagata gaaatgttgc ccccaaaatt
     4621 attatccagc tggatataat taatcaaata aaaaggatca aaaatgtagt gacccccaaa
     4681 agttagactt ttttggagtg gagaaatcca ctcttttttt catgctctaa aatgatattt
     4741 ttacacaacc gtaacggttg ttttacgaaa ttggacggga ctttgccagt ttaatttctg
     4801 ttttatccgt tcattattgt agtaatagat atagctatcg atggctgatt ttaattcctc
     4861 gaaacagtta taaactttcc cgtgaaaaat ctcttgtttt aataagccaa aaaaattctc
     4921 cataagtgaa ttatctaagc agttcccttt ccgagacatg ctttgaaaga ttttgtactc
     4981 cttcaatttg tttctataag ctttcatttg atacgaccaa ccttgatctg agtgaaacgt
     5041 acgacgatag ggacaatcgt tagtggtctg gatagcttcc tccaaacctt ccattaccgc
     5101 acgtgtttct gaatggtggt agcgaccgat ttgaacggct agggcggaaa tacggtgcaa
     5161 atgcacggtg gtttcatgca atgcttacag ggcgtatttt gtaagggtat gtaacggaga
     5221 taatcccgac ggtaatggat gtgagaggcc aagccgacgc tgacggtagg gcaaacaaac
     5281 atagttgcac tctagcgatc gttatttatt tttaacggtc aaactagggt acaactatgc
     5341 ttaaaacact tcgcctcaac ttctggtagc aaaagtgatt ttgaaagtca aaatattttt
     5401 tatgagttat ttata
//
LOCUS       DAJSVD010000141         5410 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSVD010000141 DAJSVD010000000
VERSION     DAJSVD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.144"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..533)
                     /locus_tag="ON498_002602"
     CDS             complement(<1..533)
                     /locus_tag="ON498_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940818.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCST"
     gene            731..1972
                     /gene="sstT"
                     /locus_tag="ON498_002603"
     CDS             731..1972
                     /gene="sstT"
                     /locus_tag="ON498_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR3940819.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            2119..2754
                     /locus_tag="ON498_002604"
     CDS             2119..2754
                     /locus_tag="ON498_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3940820.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            2945..3685
                     /gene="truA"
                     /locus_tag="ON498_002605"
     CDS             2945..3685
                     /gene="truA"
                     /locus_tag="ON498_002605"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3940821.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3830..5209)
                     /locus_tag="ON498_002606"
     CDS             complement(3830..5209)
                     /locus_tag="ON498_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940822.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNS
                     WKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHP
                     DMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKL
                     VQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQ
                     PRREVQHSH"
BASE COUNT         1799 a          809 c         1046 g         1756 t
ORIGIN      
        1 gtagagcagc caactaatcc aattgttaaa aataatagta gatatttaat tgttgttttc
       61 gataatttat ttaaaaatcc aactgtataa ggaatactac aaaaaagcaa agtacaagaa
      121 acagcataag taaagtaatt ctgactgata ggtgttagga taagccaata atcacgaata
      181 ttcaagctgc cagcttgtac gtaaaaaaag atattgcaaa ctagtgtggc tattaggaat
      241 ataaaggcaa aacgacttaa ataatgtaaa acttttaaac gggcgatttt ttgatattgg
      301 taacccatgt aaagtgggat caaatttgca ccaataacaa cggaagtttg taaaaatgta
      361 tagatgtaga atcgaccagg gtttgttgca tatgtaggaa tagtgactgc tgaaatcaat
      421 atacgctgta aaaccatcat tatacaagcg acaaagattg tcaggaagcc ataactctcc
      481 ttctttattt ttttcaagaa taaacacctc acaaaaaaat tggacaaaaa cataagaaaa
      541 cgtttcctga taagttgccc attaataaca tcctaactta gaatatctca aaaaacaacg
      601 tgtagacaga aaaagtacaa aattagcaag aaatggttgt ttgtattgtt agaattttta
      661 gaaaattata cctttagttt ctttgcataa aatgatacaa tgattgttaa aattgtgaaa
      721 gggtggtcat ttgttagaga agtttttgga tatgtcgtta gttcaacgta ttgttgttgg
      781 tattttagtc ggaatattgt ttggtatctt tttccccaca tggacattta tttcaatatt
      841 gggaacgttg tttgtcagtt gcttgaaggc aatcgcacct attctggttt ttttcttgac
      901 gatggcgtca attgccaagc ataaaattgg gaataagaca ttcgtgaagc ctattttgat
      961 cttatattta gtgggaacat tgctttctgc acttgtagca gttattgtaa gttcaatttt
     1021 tactgttcct attactttac aagaaacagt atctgaaaaa gcaccgcaaa gtttggaaag
     1081 tgttttatct actatgttga cgaatgtcac acaaaatcct gttcaatctc ttattgatgc
     1141 gaattacctt ggcgtcttat tctgggctgt attgttggga ctagcttttc gagcacgttc
     1201 agaatcgaca aaagagttgg tggatcagat atctatagcg ttgtcacaag tcgtacaagt
     1261 gattattgca tttgctccga tagggatttt aggattggtt tatcaatcaa ttgctacgac
     1321 tggaatcgct ggattggcag agtatctgca gcttttactt gttttgatcg gtacgatgct
     1381 gtttgtcgca ttagttgtgt acccattcct cacgtttttg tttattaaag aaaatcccta
     1441 tccattgatt ttcttttgtt tgaaagaaag cgcacttccc gctttcttta cccgaagttc
     1501 tgccgcaaat atcccgataa acatgatgtt ggctgaaaga ttgaaattga caaaagagtc
     1561 ttattccata tcgatcccgt taggtgctac gatcaatatg ggcggtgccg cgattaccat
     1621 ttccttgatg acgttgactg ctgtacattc tttagggatt gaagcacctt ttgttttgaa
     1681 agtcctttta tgtattttgt ctgctttagc tgcttgtgga gcttcaggtg ttgcgggcgg
     1741 ttcactgtta ttgattccat tagcatgtag tttgtttggt atctctaatg atgtcgcgat
     1801 gcaaatcgtc ggtattggat ttattatcgg tgttatccag gattcaatgg aaactgcctt
     1861 aaattcgtca agtgatctgt tgtttactgc aataggagag ctaggcgcgc gtaaaagaag
     1921 tggggaaaaa atcaaattga aagattgttt agatggatta agtgaacgtt gaacgttaga
     1981 tatttagtat ttttaaaatg attcgagttt ttaatgagtt atatagatgt tcgtgtaaat
     2041 agaggtttct gttaaatcaa gataaagtcg tttataataa caattaaaag cataggtaat
     2101 tgttattgga ggcgtatcat ggatttttta cgtaaatttg gttttttctt tttattatta
     2161 ggcgtgctga gtgatttttt gactccgtac gtattaggat tgtattatcc tgaattagat
     2221 caaatgaaga gcgtgatcag tgtatttgga gaggtttcca gtcctgtaag aaaagcgttt
     2281 cttatttggt ctgtggtttc agggatcttt ttcatcctgt cattgcctgc attgtatgct
     2341 gcatttgtta gtacatctcg tgtgttagct accctagtta cagcagccat cgcgttctat
     2401 ggtataggtg attgtatctt tacaggtttg ttcagtatca atacccatga atcttcgtgg
     2461 aatgtctcga catgggtcca taatatcgga tcaggtttag gttacagtgg ttttttattg
     2521 tttccagttc ttcttttctt gatctatcga aaaaatgggc aaatggacgt aagccactta
     2581 taccttggac tgtctgtgat cagtttgctt tttgcggcga tttatggact ggcaagaatt
     2641 ccttctataa attcttggcc attactgaat aaaataggtt tttgccaaag aatcagtttt
     2701 ttctttaatt atgtgcccat tgcctggttg agtatcttgc atctcaaaca gtaaaacttg
     2761 agccgtcaaa ttgaacagtc agcggatact tcatccggtg gctgtttttt gtttttctga
     2821 aatatatatt tccacagttt tcttgtatag cttctgtctt agagaagcaa ggagctatat
     2881 tttgtgctac aatcttaaaa aggtagagag aagaccaaat ggaagacaga ggagccacta
     2941 aaatatgaga aacatcaagt tgacaatcga atatgacgga aaaagatatt caggatggca
     3001 aagacttgga gatgacgata aaaccattca aggaaaaatc gaaaaagtat tgcatcagat
     3061 gacaaatgaa gagatagaaa ttattggatc aggaagaaca gatgctggca cccatgcgcg
     3121 aggacaagta gcaaatttta aaacaaacac agaaattgga ctggcagaaa tgatcgactt
     3181 tatgaatcgt taccttccgc gggatattgt catcaaaaga atcgaagaaa tgcctgagcg
     3241 cttccattca agatataatg cggtgggcaa aaaatacagc tattatgttt ggaacaacgt
     3301 gattccttca gcatttgagc gaaaccacag cttttatttc cctcaagagt tggatatgga
     3361 caagctgaat gcagcttgcg aaaaattagt gggtacacat gatttcattg gtttttctgc
     3421 attgaaaaaa tcaaaaaaat ccacagtgcg tacgattgat gaattatcta tcgaacgaaa
     3481 gggagaaatg cttcatttta cttttgtcgg agacggattc ttatataaaa tggtgcggat
     3541 tatcatgggg acattactag aaatcggcac aggtgccatg ccgatagaaa caatcgatga
     3601 aatatttgaa agcaaagtgc ggcaacatgc aggagaaacc gtccctgcac aaggtctgtt
     3661 tttggatgaa gtttattatc gataaaaata ctttgataat ggatgaaacg catgtttcac
     3721 agtataaata tcgatgttaa ataatgtatg aagcaaatta aagaaatata aaaaaagact
     3781 ttttagcctg tttatgtgta cagctaaaaa gtctttctta aataaaaatc tagtgagaat
     3841 gttgtacttc tcttctcggt tgtttttgtt tagaaaaagt aaatcgataa agtaaaaata
     3901 aaatcaaagt aacgatagat aaatatcttg aagcaattgc tagttttgtg tactgataag
     3961 taataacgac tttatttata cctggaggaa tagttaattc agttgtgcca aacttagata
     4021 attttgtttg tactagtttg ccattaatag aagccacttg ccctttataa cgataaatag
     4081 gtgtcgtcaa gacagtattt ttattattcg aattatctaa ttcaattgta agtttgttac
     4141 tcataaaatg agttgttgga tttatttcct ggttacctag taaatattga tcattattaa
     4201 tcatatcagg atgatagatt gactctttgt ttgcataatc agtatgattg taattttcag
     4261 ctatagcaac agcgtcttca ggtgctataa gtgttaatga atctttgtta gcatggatag
     4321 aattgcttac tcctgagaga tgcaatatta gtgcaagaaa cgtaaagacc attattttcc
     4381 atgaattatt tttagtagaa aaaatataat agatcactat actcatcgta taggctataa
     4441 ataaagaaga aaaaccattt aaccgccaga caaattgtag actcttaact ggtgtatggt
     4501 taaaaagttg ccaaggaaat aaattggttg aacaaaaaag aacaaataca ccaagaaaga
     4561 aaataaataa atcatcagca gttaattttt ttataaaaat aaaagcacca atcaatccga
     4621 gaaataagaa taatccaata gtatagttgt tatagttatt atttaaaata tgtgtcagca
     4681 tatcactagg agccatacca cttaattctt ttcccagtgg gactttcaat tcttgtgctc
     4741 tagattgttg aataaaagga attaagaatc ccgcacttaa aaaaaaggtg aggacggtag
     4801 cttttaaaag acttaagagt ctagcgattt tttggtccca aaaataaaaa gaaagaaaca
     4861 acgttcctcc aataaataca gaaaccatag cgacagaaag taaatgagta tacactacta
     4921 gcgtcatgcc aatagacaac cagtaccatt tttggtaatc tctaatataa atttcgtaac
     4981 accccattag tatcaaaggc aaaaatgtta aagcgacagc ttctcccaat gaagcacgat
     5041 ggaatatgtc aatggcacga taggcggaaa atgtataaat aatcgaaaat aacagcgaaa
     5101 tataacgatt gttttttatt tgtttcatag aaaaataaga aacgaccatt gttaaaaaag
     5161 tgataaaaaa atagtagata ttataactta aaacaagatt tcccatcatt ttataaaata
     5221 aaaaagctgg ataaagagta agccatggat aaaaaatatt catcattgtt ccatgatgat
     5281 cgaagttatt gaagtttact ggagaactcc atacattatc tagtcctatg attcgagata
     5341 aatgaaagta agtatcctga ttgacactat gatagattcc cttagacatg aatagaggga
     5401 tgacgtaagc
//
LOCUS       DAJSVD010000142         5247 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSVD010000142 DAJSVD010000000
VERSION     DAJSVD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.145"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(207..1169)
                     /locus_tag="ON498_002607"
     CDS             complement(207..1169)
                     /locus_tag="ON498_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3940823.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ON498_002608"
     CDS             complement(1171..2610)
                     /locus_tag="ON498_002608"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR3940824.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ON498_002609"
     CDS             2795..4741
                     /locus_tag="ON498_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR3940825.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5247
                     /locus_tag="ON498_002610"
     CDS             5005..>5247
                     /locus_tag="ON498_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940826.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1697 a          888 c          867 g         1795 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DAJSVD010000143         5244 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSVD010000143 DAJSVD010000000
VERSION     DAJSVD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.146"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            34..2187
                     /locus_tag="ON498_002611"
     CDS             34..2187
                     /locus_tag="ON498_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR3940827.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2156..3571
                     /locus_tag="ON498_002612"
     CDS             2156..3571
                     /locus_tag="ON498_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR3940828.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3604..4638
                     /gene="sppA"
                     /locus_tag="ON498_002613"
     CDS             3604..4638
                     /gene="sppA"
                     /locus_tag="ON498_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3940829.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4640..5161
                     /locus_tag="ON498_002614"
     CDS             4640..5161
                     /locus_tag="ON498_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3940830.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
BASE COUNT         1892 a          720 c         1003 g         1629 t
ORIGIN      
        1 taaaaatttt ttagaaggag ttgtgttaga taagtgataa aatcatttcc ttttgtgcag
       61 caacaagatg aaaaagattg tggtgttgct tgcctttcga tgattttaaa atattttaaa
      121 actgatatcc cgattcataa attacgggac ctatctggga ctgatttaga gggtacttca
      181 gcatttggat tgaaaaagac atttgaaaaa ttgtcttttg attgtatggc tattcaagca
      241 gatagtgaga tctggaaaga aagagatctt atcttaccat taattgcaca tatattggtt
      301 gataacagct atatgcacta cgttgtaatt tataaaacga agggaaacgt actatggatt
      361 gcagatcccg ctaatggaaa aaatagaaaa acgattgaag cgtttcaaaa agaatggaca
      421 ggtattcttt tattacctat gcctagagaa acttatattc ctattaaaga aaaagtagct
      481 ggattaagtt cttttttgcc tataatttgg aaacaaaaag gattagtatt ccacatcgtt
      541 ttagcatcga ttttcattac gttttttggc ataggaagct catattattt tcaaggtatt
      601 cttgattttt tcatccctaa tcaagcacgt tcaactttaa atataatttc cattggcttg
      661 atcgctgtct atttatttcg ggtattgttt gaatatagtc gtagttatct tcttattatt
      721 ttaggacagc gaatgagtat ggcaattatg cttcagtatt tcaaacatgt gttaacttta
      781 ccaatgaatt tttttgctac gagaaaatca ggggaaatta tttctcgttt tcttgatgcc
      841 agtaagatta ttgatgcttt agcaagtgct acgttatcaa tatttttaga tattggtatg
      901 gtgttgcttg ttggggttac gttagctatt cagaatagta cgttgttttt aatcactcta
      961 tgctctttgc ctttttatgt agttgcaata ttcgctttcg tgaaaagcta tgaaaaatcg
     1021 aacagggaag aaatggcagc cggtgcagct gtaaattcta gtatcataga aagtcttaaa
     1081 ggaatagaaa caattaaagc atatagcggt gaagagaaag tatataaccg tatagaccat
     1141 gaatttgtta aattaatgaa aaaatcattt cgtacggtta cgcttgataa tattcaacaa
     1201 ggaattaaac atttgattca attaattagc agtgcattaa ttttatggct aggttcttat
     1261 tttgttatgg ataatagaat tagtctaggt caacttatca catacaatgc tttactagta
     1321 tttttgacag atcctttaca aaacattatt aatttacaag tgaaaatgca aacggctcaa
     1381 gttgcaaata atcgtttaaa tgaaattttc gcgattgatt cagaaaaatc gaaccagtct
     1441 tttgaatata tgctttctga acaattattt caacaagata tcactgtaaa aaacgtttca
     1501 ttctcgtata gcatgaaggc cccaacgttg aacgaaattt catgtacaat tccttcgcac
     1561 agtaaaacgg ctatagttgg agttagtggt tctggaaaat caactcttgc aaagttattt
     1621 gttcgttttt atgaagcatc agaaggatcg atccattatg gaaacattaa tcacttagac
     1681 attccttttc agatattacg cgatcgagtg acatatttgc ctcaagagtc atttttcttt
     1741 agtggaacaa tcattgagaa tcttatattc ggtttagata gcccaccaag tttcgaacaa
     1801 ataatagaag tttgtgagat agtccaacta aatgaattta ttaatcaaca gccattacgt
     1861 tacgagacat tgttggaaga aggtggaaat aatctttcag gagggcaacg acaacgatta
     1921 gccttagcac gggctttatt aaaaaatccg gacatattaa ttttagatga agcaactagt
     1981 ggattagata ccttgttaga acatgcaatt atgaattatc tcctcaattt aaaagaaaaa
     2041 acgattattt ttattgcgca tcatttacca atagcaaaag catgtgatca aattttagtt
     2101 ctccatgaag ggcatttggt agaaaaagga acgcacgatg aacttcgttt tgccgatggt
     2161 atctatcaaa aattatggga aatctaggag agggaaacaa tttatgcaga ataataattg
     2221 gttcgaacat tctagtatct atactaaaca acatcatagc ttctaccgtt gggtgttata
     2281 ccctgtaatt ctttttttat gtattattgg attgttttta acctttgcta aaaaagaggt
     2341 tgttattcgg acaacggctc aattgaaccc cgaaaaaata gagaagttac aagtgccatt
     2401 agaagctaaa ataatggaaa acttcctaaa agaaaatcaa tttgttcata aggacgatgt
     2461 gttggttaga ttggattgtt ctttaataga gaatgaaaag gctcagatag aacaagaaaa
     2521 ccaacgtatt actcaacaaa taaagatggc tcagctattt attgaaagta taagtaaagg
     2581 aaaaaatttg ttttcaacgg atgacagttt tggctacagt aatcaattaa agagcatgtt
     2641 gtcagaaaaa gaatcactcc gctacgcttt gaagcaaagt gaattaaatg atcaaaagca
     2701 attagaagta tacgaaaaga caaaaagaca actagaaaaa caaattgaga gttcagatag
     2761 taaattacaa gaatggcaac aagtacaggt agcttggagt aataatcaat cattaaaaga
     2821 tttttcaaaa gaaatgatgg caaactatga gaattggcaa gaacaactaa ataatgtttc
     2881 tgatgatcaa aaaaatcaag tgaaactgac aatttcagca agcataaatg aacaaattga
     2941 gcaactaaaa aaagaagtag aacagtatca gtcagaaaaa gctaaattag ttaaaccaac
     3001 tacttctgag aatgacagaa ttagtcaaac ggaaaaagga aagcaagagc tagaacaagc
     3061 aataacaact gtaaaacaaa cagttacaga gctaaatgaa aaacaagaga agaaccagtc
     3121 agttatcaaa agtttggacg atcaactttc aaaaggaata ctaaaagctc ctgtatctgg
     3181 aacggttcat ttaaatgaag aaacaaaggg acaagcagaa ctagccaaag gaacagtgct
     3241 tgcagaaatt tatccgatcc acgaaaaatc aaaaatgaaa tttacagcac ttttacctgc
     3301 gaatgaaagt acctatatta aagaagggat gaaggttcat tttaaattgg atcaaaaagg
     3361 tggggctccg attacgatcg acggaacttt agatgaaatt tcagaaaatt ctactgccac
     3421 tgagaaaggt gttttttatg cagtgaaagg aacgttaaaa acaactcaat cgtttccgtt
     3481 tcgttatgga ttaactggag agttgtcttt aattgtagga gaaaaaacat attggcaaca
     3541 aattgtcgat gtgtttctaa ataaaaaata attattttta ctaagaatat aggaggagtt
     3601 tatatgaata aaagaagatg gattgctgtg ggagtagcgt gcggattatt gttactatcc
     3661 attatcgtcg cgctaattcc aggaaaagat aaagaggaag catcaaatac aaccctaaca
     3721 ggaataaata agatttttta cggatctaac gaaatcacag aagaaacgtt agaagaaggc
     3781 gcatcaaata aaaagattgt caaactatcc gtcaatggag ttattgcaga tacaggtgaa
     3841 agcaatcttt ttagtaggga acaatataac catcagaatt ttttaacaca gctaaagaaa
     3901 attcaagaag acaaagcggt taagggagtg ttattagaag taaattcacc tgggggagga
     3961 atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct agatattcct
     4021 atctatactg catttaaaaa tacagcggca agtggagggt actacatttc tgctggttca
     4081 gataaaatat ttgctacgga agaaacaaca actggatcaa ttggtgtcat tatttctggt
     4141 ttaaattatt ctggcttatt agaaaaactt ggcgtaactg atgcgacgta taaaagtggt
     4201 gcattaaaag atatgatgtc acctcaacac aaaccttcag aggaagagaa taaagttata
     4261 caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa aggaagaaat
     4321 atggatacta atgcagtgaa agaacttgcg gatggaagaa tttatgatgg taaccaagca
     4381 gtagaaaatg ggttggtgga tcaaattggt tactctgaag atgcattaga ttccctaaaa
     4441 aaagagaaga aattaactga tgcgacggtt atagaatata aaaatgacac aactggcttt
     4501 gcttcttctt ggttaggtaa taagattgct gaatggcaag ggctaaaggc aactacatca
     4561 gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg atactccaaa accaatgtat
     4621 tactatggag ggctataaaa tgaataagaa gagtggcatt gaaactttgc ctaattatgt
     4681 atttgcggga ttttggttac gattttttgc ttttcttatt gatttaattt gtattagcat
     4741 catcacccat gctactatag gtctctttta caaaatcatg ggactggaat ctgacaaatc
     4801 cattattact ttttatggta tagcgtcatt agctatttat ttaacctatt ttgtactttt
     4861 gactaagctg aattcaggac aaacaattgg gaaaatgatt tttgggataa gagtagtgag
     4921 ctttgaggaa aaagagctaa gttggcaaac tatctttatt cgtgagtgta tatgtaggtt
     4981 tatattaaaa aattttgtat ttcaaattgg gtatttggtg gctgctttca cgcccaagaa
     5041 acaacatatt ggggattatt tttctaacac gtcagtggtt gttattcgtt cattagaagc
     5101 tattaactat ggtcaaaaaa tttctaattc taaaacactt aatctggaga gaattaaatg
     5161 aataagagaa aaacagagtt tattttgatt ttaattagtg gaataacagg gattttatgt
     5221 agtcttttag cattgatggg tggt
//
LOCUS       DAJSVD010000144         5192 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSVD010000144 DAJSVD010000000
VERSION     DAJSVD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.147"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(44..502)
                     /locus_tag="ON498_002615"
     CDS             complement(44..502)
                     /locus_tag="ON498_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3940831.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            complement(809..1906)
                     /locus_tag="ON498_002616"
     CDS             complement(809..1906)
                     /locus_tag="ON498_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3940832.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(2014..4041)
                     /locus_tag="ON498_002617"
     CDS             complement(2014..4041)
                     /locus_tag="ON498_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3940833.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4186..4917)
                     /locus_tag="ON498_002618"
     CDS             complement(4186..4917)
                     /locus_tag="ON498_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3940834.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1469 a         1049 c          818 g         1856 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat tagaattata tcatttacta gtatgcattt
      721 ttattaataa aatggtatat tttatattgt tataaaaaga gcccaaccat cctacaataa
      781 taaaaattta ctatttgtta tcttaacctt acttttcttt ctcttctttt tttctcaaaa
      841 caaacaaaag tatgcataat aaaataaaca ataagaaaag tgaagctact aagatataaa
      901 tccaattatt gtctttttta atcgtaacat ccgtcgtgtt caattccttg gctacttttc
      961 cgtctattgt aaaatctttt tcaaataccc attgattggt atagttgatc gtagtatttc
     1021 ctttttttct cgaaaatttt ccattttcat tttcatttcc taaaacagtc attgtaaggt
     1081 gatattcacc tggggttagc ggttgaccat ttaaagcagt tggaaaagaa aaatttgtat
     1141 tcggcgcaat ctgcaatcct tctttttcct cttgatataa aacttcagaa tgtccttttt
     1201 ttgtaatttt tgtttcaata actacttgat tgatataggt tttttgatca ttttgaacgt
     1261 ttgctaagat gacatttctt gcattgattt gatctggttt aacttcatga agagtaaggt
     1321 tcggagcaac ttcgttcagt gtctgttgca tcaacaaagc aataatatag gtgtattcat
     1381 tctcaatcga taagctttga tccttagttt ctgtttggtc cttttccgtc tggatttctt
     1441 ggaatgtgat cccaccagcc aatacaccat caaatcgctc attcggcatc ctgacatgaa
     1501 aagcaacggt ttggacactt ttaggtttta gcgtcactat ctgtggatag ctcacataat
     1561 ctaccagatc aaagatcagg ctcatgtctt tttcaatctc gttttttccg tattcaacca
     1621 tcacatttga attggtggtt gcgctattca cagaaatatc gattttcact tctttttctg
     1681 tatcatttct caattccgct tttacttcta cttcctgatc cggatcaagt tgaagatcga
     1741 agtaagtttt tctcttgtcg atttgattct ctgaagttat cggcgtcact gaaaaattaa
     1801 atgcactagc tgacactgat atattgttaa aaagtgcccc taaaataact aggagtgtta
     1861 taaataattg ttgttttggt ctcaagaaaa tcgctccttt aatcatatgt aagagaagac
     1921 tataacaaaa tcttgtctct gtgggacaca agagcctatg gtacctgtgc ccttcttgaa
     1981 actcaataat tttgttatat tcttctcttc ctgttaattt cctgggacat ctgcaagtat
     2041 ccaagatagt ttcccctcaa aggttccgat tctttggttt tctacaggaa tatttattct
     2101 gattccacga tgagctgtag aatctaaaag agaggacaaa tcgctttcac ccttttgatc
     2161 actttcgaaa atcgtaacag cggatgtccc tataaccgtt gtattttcag aggtaacaaa
     2221 ggaaagagct tctggaagct caaaatctcc atttgtaaga actttctctt gtttcaattg
     2281 taatttccaa cttccaaaag cccgttcatc tataattttc aacccacctg aaaactgtcc
     2341 gaattcactt aatggtctac tggatatttt atgttttcca aaattgacat attgaggagc
     2401 agttgagaga taaagtttgc cttttctgta ataaaaatag atatcctctg tattttcgga
     2461 gaagatgcct tctgtattcg ctgaatccga gtcaaaaaca tacccttcaa tatcaatagg
     2521 agtttcttta tacttctctc ctattttccc agtgattgtt ttcatttcag caatatcaat
     2581 ctgactgtct ttatctttat aataaacatg gatatctatc tccttcagct ttgcgatctt
     2641 taggtttatt tcattcgatt gctcaatcaa attcccgttt tcaaggtatt caaaatgcat
     2701 agtgagtact ttttcaggaa ttacttcctg caaagttgtc tccaattcga ttattaacgt
     2761 atctccagca ataaaataat ctgtcagatt tagcgtgact cgatttccgg ttatcaagat
     2821 atctttggca tcaatttgat ttccattgat caagatatgc tcgggaatag ttaattcttg
     2881 tgggaggtcg atactagtct gcattttctt atattcaaga aaatccccct cgaccttcaa
     2941 tgtatttctg atagttatct tatcgtttcc tccagcttca gcgatggccc cataatcact
     3001 atcgttgact tttacatcag ctgttgatgt gacattcgat ggaagtcgcg tgacagcgac
     3061 agcattttct tgaaaaaaag tacctgttga agaagtaaac ccccaattta cttctgtaga
     3121 accaaactgc ctaatgagat caggtataat ccgagaagag cgaccgacac cttccaaatc
     3181 ataagtaaga cttctttgct ccgcgtccca tttaattatt aacaagcgcc atgtgccatt
     3241 agaaagaaat gtaggagcta ccgtcacgcc agtatgctct ccagaataat tattattatt
     3301 tggtttaggc gtgacaaaag cgacatgtcc atgccctcta ttatcagcag ttatctctcg
     3361 atccattcga tcagaagaac cactattttt atacgtatca aactcaatcg agagcgcatt
     3421 cgcaatgtaa ggttggccag aacgagtaga atatgagcca attcccatac ctggcgatcc
     3481 aataacttga ctgggattac tagccattct aggatcgcca gtcaatgtaa aggtcatgcc
     3541 atctcccgct ctatctcgtt gatttcctaa gtataaataa gatttaaatg taaaatccga
     3601 gagtaaacta atcttcttat ttgaccaaat agctcctctt tgagaccttt ctgctgggtt
     3661 caactgcagt aacttcccat tttccaatat ggacgtgcta gtaccgatag gtgtttgaaa
     3721 aattttatcg atagaaataa tattgggatc gtcaggcagc tcaaccgcag aaattggtgc
     3781 atcttctagt ccttgtttgt ccgaaccctc agcttctacg ttgtcagtgg tctctccact
     3841 actcgaaata cttaaatcct cactgctttc ttgtgtctgg ttggattcta ttaaactgtc
     3901 gaatgtcgaa gtgagtttgc tttcttcgtc tcttctattt ccatgctcca actgattcaa
     3961 ttccaaagag taccctatga tcggaaagat attagtcact aagagactgc ttaaaatgag
     4021 cacgatcctc ctctttttca atatatcttt tcctcctgat cgttaaattc ctagatgaac
     4081 tattcgtatc caaacaaatt tagcctagcc tttgacaatt tttgctcgat tacctcgcaa
     4141 gacaatatga ctacgtattt atttttaaca gttcttttca gtaaactagt tgttaggtgt
     4201 atcttctaat gtccaaacta atttagtgga atattgttgc gctaacttga cagatttacc
     4261 gggtaccgaa agctgtaccg catcttgatt ttcattttca tttgcaccaa atcgatagac
     4321 ccaagtcccc acacctgttc ctgatttagc attaattgct aacgtattgt ttcctcccgg
     4381 tgtcaacgtt aattcagaat tcaccgtttg cggcgtatat gttggatcaa ccaaagaaac
     4441 agcctgccct ttcgtaaatt tcagctgtgc tccaactagt tcatccccac tggcagtttt
     4501 aaactgttct ggttgagaga cacttaattt ccacccagat aaggtacttc tcctatctgt
     4561 cacttggaca tatgtcggtt ttctagaccc atcgttcatc acatcagcag ctgcataata
     4621 cgttttatcc gcagtagaaa ttttttgagt cccaaatttg aattttgatc cataatcaat
     4681 cgataatgcc cctcctgttc cagggcttgg aggatctgat ggatcgacag gtttattggg
     4741 atcaggattc tctggatcaa caggaggagt tacaccctca tctcctgctt caaatgtaat
     4801 cgttccttct gtgttatatt cctttgggta tacttcagtt gcagaaacaa aagtcgtact
     4861 tgctaccatg ctgaataaca tcattgctgc tgttaatacg atttcttttt tcatcattga
     4921 cattcctctt tctttattta ctaaacatat cagtcgattg gattaatcca tagcaaaatt
     4981 ctgttttacg atacttatga aataaaagaa catagaccta tatttggtcc tatgtatcga
     5041 ttagttataa atgattatca gcccgatttg atttctagtt tttattgcaa cttgatgcaa
     5101 acacaatcta gaaatacagc tgtcatagcc tttaaaataa ttcgtaagtc cccctcctat
     5161 ctatcaaaat gtgacttgac gagagtgtaa aa
//
LOCUS       DAJSVD010000145         5174 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSVD010000145 DAJSVD010000000
VERSION     DAJSVD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.148"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..1309)
                     /gene="uxaC"
                     /locus_tag="ON498_002619"
     CDS             complement(<1..1309)
                     /gene="uxaC"
                     /locus_tag="ON498_002619"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3940835.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSE"
     gene            complement(1383..2462)
                     /gene="uxuA"
                     /locus_tag="ON498_002620"
     CDS             complement(1383..2462)
                     /gene="uxuA"
                     /locus_tag="ON498_002620"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3940836.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(2465..4096)
                     /locus_tag="ON498_002621"
     CDS             complement(2465..4096)
                     /locus_tag="ON498_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3940837.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            4400..5071
                     /locus_tag="ON498_002622"
     CDS             4400..5071
                     /locus_tag="ON498_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3940838.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         1659 a         1019 c          730 g         1766 t
ORIGIN      
        1 actcgcttag taattgacat aatactcgac gaaaatactc atggcgagga tagcttagaa
       61 aacttcgaga atcagtcaac atacctgtaa aattcgacaa caaactttgt tgtgccatcg
      121 tagtcaactg ttgacgcatt ccttcagctg tatcattgaa ccaccaagca catcccaatt
      181 ggattttttg aatcataccc ccttggaagc tttgcattgc tgttgctagt ggaagccaat
      241 cagttggatt taatgaatat aaaattgttt ttggtaagca cccactcttt tcagcggtat
      301 cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga
      361 catctactcc aatttgttcc tttaatatac ttgaattatt tcttaaagca tttaaatgca
      421 tttgcatcgt ccaaccatat cttgtatact cagacatcaa atgtaattga atacctgtaa
      481 caaattgtgc aatttctgat aaatggaaat ccgtttttcc tgctaaagct tttattaaca
      541 gattgtcgag tattttttca tctgctattt cataaaaata agtattcaaa ccgtgatctg
      601 ctaatcgaca gccaatttga tgaaaatgat ccactcgttg gctcaatgct tgcttcaacc
      661 cattaaagtc tttgattggt atagccgctg tttgactaag ttttaaaata tagttcgaaa
      721 agcctgatga tttgatactc cagaccttat ctggacggaa agtaggtaat accttgaaag
      781 aattatcctg tttagaaagc tcagtatgat accacaagtc atctgtagga tcatccgttg
      841 tacagatggc tttcacatcc atcatctcca acaattcttt cgggcgatag gaatccgttt
      901 gaattttttc gttagcacgt cgccaaattt ctggtgcatt cttctcagaa attgtcaaat
      961 caatatcaaa gtaacgttgt aattccaaat gagaccattc aaaaatagga ttacctggcg
     1021 ccttctccac tgtttttaca aaagctaaaa atttttcata atcatcacct ggaccactga
     1081 tcaactcttc ttctatacca ttagctcgca tcaatcgcca tttataatga tctccagctc
     1141 ctttatcatt taaccatacc tgagtcaaat tcttgtattg cttattttct agcaaatatg
     1201 ctgggtctaa atgacaatga tagtcgataa ttggttggtg ttttgcaaat ttatgaaaaa
     1261 gtcttttagc tatttcattc tctaataaaa agttcttatc taaaaacata tttaaacact
     1321 cctttccttc cgttagtttt tagaagagaa aatttttcct ctaaataaac tcccttatat
     1381 aattatttac tcgccccctt ttcaagcgct tcccaaatac catttaaata aactgcacct
     1441 agagctcggt catataatcc ataaccaggt ctaccggttt ctccccaaat catccgacca
     1501 tgatctggac gaatatagcc attaaatcca ttatcgtaca ttgtttttaa tatcttatac
     1561 atatctagag aaccatattc tgatttatgc ggcgcttcgt aaaaatcgag tccattgtca
     1621 cttaaatatt tgatattacg aacatgtgca aaaggaatcc gatcccgttg acaaaattca
     1681 gatagaattt catagacatc attatctggt cgttctgcaa tacttccagt acataatgta
     1741 ataccattag aaggagaatc cactacatta cacaaccaat ctaaatcgtc acgattttta
     1801 acaatacgag gtaatccgaa aatggaatat ggtggatcat ctgggtgaac tgccatttta
     1861 atcccaactt cttcacatgt cgggataatt gctttgagaa aatatgcaaa gttctctcgt
     1921 aatttctctt catcaacttc tttataggct tctaataatt gcttaatttg tgataaccga
     1981 tgaggttccc aacccggcaa ttcaaaatta ttagtagaat tttctacttc tctaataatt
     2041 tcctccggat cagtagggat attttttgaa atataggcta acgtagaaga cccatcatct
     2101 aaacgataat ccaaatccga ttttacccaa tcaaatactg gcataaaatt ataacagata
     2161 acctcaatcc caaattctgc caaattgcga atcgtctgtt tataattctc aatgtattga
     2221 tctcgtgttg gtaaaccaat tttgatatca tcatgaacat taacactctc aataactttc
     2281 aaggtaagtc cattggcttc gacttcatct tttaaagatt taattcgttc tctcggccaa
     2341 acctctccgg ctggtatatc cattaatgtt ccaacaattc ctttcattgc tgggatctga
     2401 cgaatttctt gtagtgagat tttttcttca tttgccccat accatctaaa agtcatttcc
     2461 atagttactt tcctccaact tctgttatat atttattgat tgttttacga actgcttgag
     2521 gacctactag catttcgcaa aagatttttt cagttttctt tcctacccca acttgatata
     2581 aatctaaacc aaagatttct ttattttcta aaatttcctg taccgcacaa tgaatctttg
     2641 aaggatcttg ttcacctaat ttcaatactg ataatttatc ctgcaactct accaataacg
     2701 gatcaggact tggcacaaaa ttttcccctt gatcatcgac tgccagtaaa taacgtaacc
     2761 aagcagcaat agcgatagga ataaatgtta gattttctgc atttccgtct ttttcgacat
     2821 actttttaat tgtttcacca tagcgaatcg ctactttttg agaagtatct gttgcaattc
     2881 gttgtggtgt atctggaata tagggattag gcaaacgttt attaattact tcatcaataa
     2941 attgttttgg atcaataatg cctggatttt caactactgg taaaccttct aaataaccta
     3001 ttcctttgat taaaccaacc aaatcatcat cctgcatctc tgctgaaatt gattgatatc
     3061 ctagcagaca acctgttact gccaaagcag tatgaagtgg attcaagctt gtcgtaacct
     3121 tcatgatgtc tgctttatca accgtctctc tatctgtcat aatcacacca gccttttcca
     3181 atttaggccg gccatttgga aaactatctt caataactaa ataatgaact acctcagtat
     3241 tagtgaaagc agcaatattg gtatgttttt ttgtgtgaat gatatctaaa tcctcgagcc
     3301 ccatcatcaa tagatcttcc tttacattct ctgaaggatt tggcgtaatt cgatcaatca
     3361 tactccaagg aaaagtcact ttttctttat cctttaaata agcaataaat ttttcagaaa
     3421 catatccttc tttttgccaa gcttctgcaa tcgtaaaaat actatcttga aaacgttgac
     3481 cattctgtga gaaattatca gtagaaacca tcgcaatcgg caactctcca gcattaaatc
     3541 tgctatataa caaggatgtt acaatactca tcgtatgttg aggattgcta ggtccagatt
     3601 tgatatcagc tttgactacc gaaaagaatt gccctttaga atcttttaaa ctgtaacctt
     3661 tttcagtaat agtaaacgtt gtaaactgta atttaggatc ttcaaaatat ttaaccactc
     3721 tttgatagtc cgctatattc ttcggattgc aatataaact atcagccgtt actgccaaaa
     3781 gtcttttttt gagttcacca tctccactca taacaatttc caaaatgtca ttgttatagt
     3841 catgataagc tttttccact acttcttcat caaatgtttc acacacaact actcctgttt
     3901 ctaattcatt ttgatctgct aagcattgag caatttctgc atgaaatcct cgataaagat
     3961 ttcctccacc aaaatgaagc catactggat gttgctggcc ttcttgctta acttctttga
     4021 catcaaaaca cggcagtaga atctgcattt cattgaattc ttcgatagaa ttttgccaat
     4081 ttttaatcga aaccataatt aacctcctca aactgatatg ttagttttga tacattttat
     4141 attttaaaac ccattgtaac cgttgtcaat ataaaaaggg aataaacttg cttttttcta
     4201 ccatatcaga atgattgtat tttttattca gaaaaataaa atactccttc tttttggact
     4261 gattttatcg aaacttattt tttctttttt tcatgtattc ttttattttt ataactagtg
     4321 tattacttca aaatttgata ttatatggca tgaacagaat taaaatatca gttaaaataa
     4381 aagttaagga gcattgtgta tgacaattaa aactagtctc caaaatcaag cttaccaatc
     4441 tattcgccga caaattatct atgctgactt agaacctgga aaaaaagtat cagaaaaaaa
     4501 attagaagaa acactttcta ttggtcgtac accaatacgt gaagcactga ttcaactccg
     4561 acaacaaggt cttgttgata caattcctca aagcggaaat tatatctcat taattgattt
     4621 agattcggcc aaaaatgctc gatttgtcag agaacatttg gaacgacaaa ttatgttaga
     4681 gtgttgtgcc aaaatgaata accaaaaaaa acggatttta gaaactataa tttcagaaca
     4741 agaaaaagct gttagtcaaa aaaatgctcg tagttttttt cataatgata atttattcca
     4801 taaagcttgt tttgaaattg cagagcgaga agaagtatgg caatggttag aaaataacaa
     4861 tacacactta gaacgatttc gatggctacg agtaaaaacg gaaggattaa agtgggaaaa
     4921 aattatggaa caacattatc aactatttga ggcattaaca aaacaagatc cagaagaagc
     4981 taactttcta acatcgttgc atcttcattt aatgcttaat gagcaaaata ttgttttagc
     5041 tactcatcca aaatatttca aaaatatata acttttaact ctttttaaat taaatggttg
     5101 ttctgatcag aaaaatatcg cttgttttct tattatgtaa tatatttcat aaactattct
     5161 gcataacaac tatt
//
LOCUS       DAJSVD010000146         5155 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSVD010000146 DAJSVD010000000
VERSION     DAJSVD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.149"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            101..712
                     /locus_tag="ON498_002623"
     CDS             101..712
                     /locus_tag="ON498_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3940839.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            785..1714
                     /locus_tag="ON498_002624"
     CDS             785..1714
                     /locus_tag="ON498_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3940840.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1783..2609
                     /locus_tag="ON498_002625"
                     /pseudo
     CDS             1783..2609
                     /locus_tag="ON498_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2657..3835)
                     /locus_tag="ON498_002626"
     CDS             complement(2657..3835)
                     /locus_tag="ON498_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3940841.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3858..4031
                     /locus_tag="ON498_002627"
     CDS             3858..4031
                     /locus_tag="ON498_002627"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940842.1"
                     /translation="MCCGNSILLKGLPFFYLHKIFYTLGLKERGLSGVKMVISDAHIG
                     LTKAVKEYLLCAS"
     gene            4062..4463
                     /locus_tag="ON498_002628"
     CDS             4062..4463
                     /locus_tag="ON498_002628"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3940843.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4670..>5155)
                     /locus_tag="ON498_002629"
     CDS             complement(4670..>5155)
                     /locus_tag="ON498_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3940844.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1628 a          871 c          996 g         1660 t
ORIGIN      
        1 atataataaa atgtattata ggcatttttt tacaagaaat agaagcttga agtttatatg
       61 aagttataaa atttaattta tattggaagg aggaaaataa atgtatttta tactacctac
      121 actttatagc cttttatgtg taatggcacc atgtattatt tatatggtat ttttttgcaa
      181 aaaaaatacc ctgaaacaca atatcctgat tttgattttt atgctgtact gttggaaagt
      241 ttttgaattg actggtacag gtggactaac agatattttt tatagacctg atacaggtag
      301 gaaattttct ctagtacagg gcagtgttaa tcttgtacca ttcagtggac taaacattag
      361 ctttatatta aatatagtaa tgtgtgtgcc cttaggattc ttactaccaa tcatatggaa
      421 aagttatagg aaaatagttc ctgttttctt ggctggggct ctattttcac taattattga
      481 agtctcacaa attataacta caagagcaac agatattgat gatttaatag caaatacctt
      541 tggtgcggtt ataggcttta tactgtgggc tgttttaagt agagtggtgt ccttgaagga
      601 caagtcatct acagtagatt atttagaacc ttatatttat atattgattg gttttaccgg
      661 aaaattctta ctgtattttc ctttctggtt tgcttttgaa gtattatcct gataattcca
      721 tagaaaataa tgagtttgag ttgagaaaga atattcttgt acacaactct ggaaaggagg
      781 aagtatgaaa agattcttaa aatgggggct tgttctttta gcgtctttga ttgtgattct
      841 aatgtgtaca gcggttgtta tttcttatgt gaatcggccg tatgcaagag ctgtttatga
      901 taggattaaa cgcattcttg acaaagattt ttatgaccat ctatatctag gttcttcatt
      961 tgaaataata aaccttgata caatggatat tcatagctat atgaaaaaag accaagaaga
     1021 attacccgcc tcattgagca aattatttac gattgattat gcgcttagtg tcctaagttt
     1081 agatgaacag attacaatta gtcctactgc acttgacatg cagaaagctg actcatctac
     1141 tgcacatctt caagaaggag atattttatc tgtttatgac attatctgtg ctatggtctt
     1201 accctcagga aatgatgcag cttatagttt ggcaatttat acaggtggaa aacttgatcc
     1261 tgatgcaaag aatgatgaag aacgctatca aagcttcatg aaaaacttag atcgttatct
     1321 aagtgagcaa ggctataaac atactcattt tactgacgct gctggctatg actccaatgc
     1381 tacaacatca gcggaagatt tgacaaaagt agctgcaaag ctactaaaga atgataaatt
     1441 tgcagaaata gttgccaagg aaacttatag agcaaaatca attaagggaa atgtttataa
     1501 ctggaatact acgaatcaat ttatgcaaca agataataga tactttactg aaggtgtaaa
     1561 aggtgtaaaa actggatcgt ttgatgggag atacaatcta tgtgtgtatt atacatctag
     1621 ttctgataac cactatttac ttgtaagttt tggttcagag agtgatagta ggcgttatgt
     1681 agatgttcaa aatctattgc ataatctgcc ttgaaatttc gtgcctttaa gcgcgtatct
     1741 aaagtttctt tgaaaagttt gagaatgaag aaagtgagca gtcgaaaaac ggttgctcac
     1801 ttttttgccc taaaatcgaa aaaatataaa aaagtccttt acaacacgtc tcaggaaaaa
     1861 ctgctaagtc aattctaata tcttgtggag caagacttgc accttttgat ataagagagc
     1921 ttagagaact aatgagcgaa gatgaactag aactcgataa aattggagat agaaaaactg
     1981 ccttgtttgt aataatatca gatacagatg atacatttaa ttttgtagtt tcaataatgt
     2041 attctcaatt atttaatcta ctatgtgata aggcagatga tgtgtatggt ggaagacttc
     2101 cagttcatgt taggtgtcta cttgatgagt ttgcaaatat cggtttgatt cctaaatttg
     2161 aaaaactaat agcgacaatt cgttcaagag aaatatcggc aagtataata ctgcaagcac
     2221 aatctcaatt aaaagcaatt tataaagacc atgcagatac aatagttggt aactgtgact
     2281 ctacactttt tctaggagga aaagaaaaaa caacagtaaa agaattatca gaaaccttag
     2341 gaaaagaaac catagaccta tataacacat cagaaacaag atcaaatcaa aaatcttttg
     2401 gtctaaatta tcaaaagact ggcaaggaac ttatgagtca agatgaaata actgtaatgg
     2461 atggtggtaa gtgtatttac cagctaagag gagcaagacc ttttctatct gataaatttg
     2521 atattacaaa gcataagaat tacaaattgt tggaagatta tgataagaaa aacttgtttg
     2581 atgtagaaga gtatttaaaa caaagatagc gtaaaatatt ttgtgtaaat aactcgtcct
     2641 tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc acttgttgga
     2701 agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg gatactagga
     2761 aacgctctag tgattcttca ttttgaaact gctcttttct gtggctgtat ttcttgattt
     2821 gcttattaaa ggattcaatc aagttcgtag agtaaatagt cttgcgtatg gctaatggaa
     2881 aatcatagaa agtgagcagg taatcgttcg aaaggatcga ttccaccatt tttggataac
     2941 tggttttcca tttttccgca aaagcaccac gtgcttccaa tgccgccttt ttagatgacg
     3001 cttgatacac cattttaaaa tcatcacaga cttccttacg atcatcgaca cgcactttgt
     3061 gactgatatt acgggaaacg tgcacacagc aatgttgaaa acgggctttg gggtagaacc
     3121 gctgcacggc ccctgccatc ccctttaagc catccgtgat gaaaaggagg acgtttttca
     3181 cgccacgttc ctggaggtct attaaaatct cttcccaaat cgtgatagat tcagttggtg
     3241 caatcgcata gctcaacact tcctttgaac cgtccgggcg aatgccaact gcgatatgaa
     3301 tggcttcctt ggcgacggtt ttccgcttta aagggatata ggttgcgtcc atataaatag
     3361 cggcataacg gtcatgaagc tcacgttcct taaatgaagt gacttcttcc gtaaatgatt
     3421 tagtcatatt ggacatggtt tggggcgtgt agtgatgccc atacattttc tcaatcaagt
     3481 ctgcgatttc cgacatggta ataccttttc ggaagaggtg aatgacggtc tcctctaagg
     3541 tatcattcgt ccgtctataa gcaggaacag tctgttgctt gaactcgccg ttgcggtcgc
     3601 gcggaatctg gagatgaagt tccccgtact cggtcttgac cgtacggtca taggagccgt
     3661 tacgagaatt acccgtgtta aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg
     3721 ttaactccgt tttgagaagc gtattgacag ctttttccaa atggacacgg aataattcat
     3781 tcaaatcgcc tttattcgct agagtcttta gaatttctgt agtaaaatcg ttcatatgga
     3841 agtcctcttt tctgtgaatg tgttgtggta actctattct actgaaggga cttccttttt
     3901 tctatttaca caaaatattt tacactctcg gattgaaaga acgcggtctt tcaggagtaa
     3961 agatggtcat ctcggatgcg catatagggc tcacaaaagc tgtgaaagaa taccttcttt
     4021 gtgcgagcta gcagagatgc caagcacatc tccttcgcaa catttccgac aaattctcca
     4081 agaaaatctc aagcgatatc aaagaagact ttaaagccat cttcaaaacg gcaagcatcg
     4141 agctggctgg agaacgtaaa caacttatta tggataaata tggggaagac aagaaagtcg
     4201 caactgcctg taaaatattg gatgagggct tcgaagatgc catgcaagta atggttttac
     4261 cagaaaatat ccgtcaacga ttgagaacaa cgaatgttct tgaaagacta aatgaagaag
     4321 tacgccgtcg tgaacgtgtt gtccgaattt tcccaaattg tgattctgtt attcgtatca
     4381 tcggtgcttt attgatggaa aaggatgacg aatggacttc tgcacctaga aaatatcttg
     4441 aatttgatcg taacgagatt tagcacatta agccgccaat agtataatat actggtgaga
     4501 agtatcaaga gacgcttcgc tttttgctct tgacacttct taccagcata ttggaccgta
     4561 gctaggctca acaaggcaaa acatcgtact ttgagatttc ctttttacac aagattttgg
     4621 acttgacaca gcccctatgg ttcttacctt gtttatgctt atttaaatct tactttatta
     4681 cagtactttc aaacttcgtt ttgtaagccg ggtattttga tatttcataa ttatgtccct
     4741 ctttttgaat atattctttg atatctgctt gttcatctac actttctttg cgtaaaatac
     4801 gttcgatcat tgagcagcag tgcatgtaaa gcaagaacaa ttgattgttt gaaaattcga
     4861 tccccattag tttttcgagt tctttgacgg ccttttgtac caatctcaaa accttcattg
     4921 catcaaggat cgttaaattt tctacgatct tgttaatcga gaaattaaag tattgagcac
     4981 aattataggc gataaatgga gcatttgatt ctatcacctg ttgttccttg gcgtattcat
     5041 aaattttttc tgccaaaaag gtttttcctg aaccgcttac acccatcaat aaaatcggta
     5101 atccgttatg aggatacaag acagccgtct tgatttgatc gatcgcttcc tttaa
//
LOCUS       DAJSVD010000147         5131 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSVD010000147 DAJSVD010000000
VERSION     DAJSVD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.150"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(30..488)
                     /locus_tag="ON498_002630"
     CDS             complement(30..488)
                     /locus_tag="ON498_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3940845.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(501..1832)
                     /locus_tag="ON498_002631"
     CDS             complement(501..1832)
                     /locus_tag="ON498_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR3940846.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1843..3285)
                     /locus_tag="ON498_002632"
     CDS             complement(1843..3285)
                     /locus_tag="ON498_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3940847.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3272..4036)
                     /locus_tag="ON498_002633"
     CDS             complement(3272..4036)
                     /locus_tag="ON498_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR3940848.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4140..5015)
                     /locus_tag="ON498_002634"
     CDS             complement(4140..5015)
                     /locus_tag="ON498_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3940849.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1548 a         1089 c          752 g         1742 t
ORIGIN      
        1 tcaccctatt taactttttt catcgtacac taaaattcaa aacaactatc tttaatcttt
       61 acttcttggt tttgttttaa taaatttcct tcaaaaatgc ctggagaaac tacaatgatc
      121 accgtatctt tttgtgcttg ctccaacttc ttcaaatcta taatagctaa cagtgtccct
      181 tcttttacct cctggttact ttcaacccgt atctgaaacg gacttccttc gagttcaact
      241 gtatctaaac ccatatggat cagtattttt ctgccagatt cactttcgat cgttatggca
      301 tgtcgggtag gaaatatatt atcgattatt cctttaactg gcgcatggat atatccatca
      361 tgattcgtta tagcgtatcc aactcctatc atttttgaag aaaagacctc gtcttttact
      421 gctgttaatg gaataatttg accactagct acagctttta gaatgtttct tgttttaaag
      481 aaatgcactt tttatcacct ctactttaat gctggccaat attttttgtt tactttgatc
      541 atttcatcta gtattttttt tgcgactctg gcatttggta cggtttgatt caatgtaaat
      601 gcttgtaaag ctttttgata agaaccttca aagtaagcat ctactagtaa cttctctact
      661 gcaacttgtg cctccatcaa tcctttatga aaatcactga ttggttttct tagtgtaatt
      721 ggctccacac cagttgcacc aacatatgcc ggtatttcaa caactgcatc tgaccgtaga
      781 ttcggaatcg ccccttgatt aggaacgatt agcataaagc gacgacgatc atcattcaac
      841 aaggaaatag ctatatcgac gatatattgt ccatgctccc caaaattaaa gttcaatact
      901 tcttccacaa taccgcggcg tattttatca gccatttctt tggtattttt ctctcgtcca
      961 tccatgacca tattagcacg tgtatactct ttgtcagtat gctcaacgac catatcagga
     1021 tataagtaat actctaagta attattcggg atattacttg gaaaattttt cgtgatgact
     1081 gacatcattt gaaaggtttt ttgccaagtt ttatctccta tattaaaatc ggcgacttgc
     1141 atttcttttg ttattagttt ttcaataatc tctggcataa tttctcgttg caattcttta
     1201 tcataaatcg atgtatacca gccaaagtgg tttaaaccat aatacgtaac gatccaattc
     1261 ttacgatcat aaccgtaatt cactgcaatc gtttcttcaa ttgaaatagt catatcacag
     1321 gcattaatca tttttacatt cggatattta cgacgaacag cttcagaaac gatcgactct
     1381 ggatttgtat aattcaaaat ccatgctttg ggggcatagt cttgtatatg atcgacaatt
     1441 tccaataaac ctttgattga gcgcatccca taagcaaatc ctccgagtcc acacgtttct
     1501 tgtcctacta atccatgttt aagtggaatc ttttcgtcct tttctcgcat ctccaatcct
     1561 ccaacacgaa tttgtgagaa aacaaaatcg catcctgtaa aagcaagctt tggatcatcc
     1621 gttgcacgaa tgcgaatatt tgaaaaacct tttcttttca gcataaattt aacaatcaag
     1681 tacatatcca tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac
     1741 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
     1801 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
     1861 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
     1921 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
     1981 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
     2041 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
     2101 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
     2161 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa
     2221 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
     2281 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
     2341 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
     2401 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
     2461 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
     2521 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
     2581 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
     2641 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
     2701 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
     2761 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
     2821 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
     2881 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
     2941 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
     3001 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
     3061 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
     3121 tccagctgca ccaattactg ggatgttcag catatcgtca gctgataaac gcatgatcaa
     3181 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
     3241 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
     3301 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
     3361 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
     3421 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
     3481 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
     3541 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
     3601 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
     3661 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
     3721 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
     3781 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
     3841 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
     3901 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
     3961 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
     4021 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
     4081 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
     4141 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
     4201 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
     4261 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
     4321 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
     4381 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
     4441 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
     4501 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
     4561 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
     4621 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
     4681 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
     4741 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
     4801 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
     4861 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
     4921 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
     4981 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     5041 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     5101 agcaccctac ataatttaca aaaatagttt a
//
LOCUS       DAJSVD010000148         5130 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSVD010000148 DAJSVD010000000
VERSION     DAJSVD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.151"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            56..478
                     /locus_tag="ON498_002635"
     CDS             56..478
                     /locus_tag="ON498_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940850.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(644..1837)
                     /locus_tag="ON498_002636"
     CDS             complement(644..1837)
                     /locus_tag="ON498_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3940851.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2061..2438
                     /locus_tag="ON498_002637"
     CDS             2061..2438
                     /locus_tag="ON498_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3940852.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2426..2764
                     /locus_tag="ON498_002638"
     CDS             2426..2764
                     /locus_tag="ON498_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3940853.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2898..3341
                     /locus_tag="ON498_002639"
     CDS             2898..3341
                     /locus_tag="ON498_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR3940854.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3603..4418
                     /locus_tag="ON498_002640"
     CDS             3603..4418
                     /locus_tag="ON498_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3940855.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4428..5090
                     /locus_tag="ON498_002641"
     CDS             4428..5090
                     /locus_tag="ON498_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3940856.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
BASE COUNT         1714 a          885 c          976 g         1555 t
ORIGIN      
        1 tatccgtttt ttttcttgta tgtagtatag taaagtagat acaggaggag aaaatatgca
       61 gactttttta acaaaagttt ctcaaaagaa aattaaaatg attcatttac tgcttgaaac
      121 agaacgttgg tgttcaatag aagaactaca aaacgaattg aaggtatcaa gtaagtctat
      181 cttgaattat ctaacagatt tagaagaatt gttccaaaaa tatcctgata aggtcatttt
      241 gaagaatgag aataatcggc gcttttctat ggaaaagcag gagaacttcc caatctacat
      301 tatttatctt catttctaca gaaaatcata taattatcat ttgattgaat tcatgtatca
      361 acatcctgag aaaaatttgg aagattatgc agacgctcaa tatacgagtg tatccacagt
      421 ttttcgttat gctaagttat tagtaaagta ctttgaaatg acggacgtca aagaatagtc
      481 gccgccagat agaaggaaat tagcagttga aaaagctgcc ctgcatcaag taatacgacc
      541 agccaaacgg aaacagtgtc tcatgttttt gatttggctg gtcttattac cgcagatgca
      601 agcttttgcc acacctcttt cttgtcattt acagactcaa ctatcaatgc tgcaagcttt
      661 cccttaatgc tgtaacacca gtaagagttg tcaagaccag cagactgact aggtaaatac
      721 cgctcaaaaa caagattacg gcagttaaac tataatgatc catcaagaaa ccgatgacga
      781 tagaagaaaa accaccaaca gctcgtccaa ggttcataat gaaattattc gccgtcgcgc
      841 ggatttctgt tggatacaaa ctactaatca ctgcgccata tccaccatac atgccattga
      901 taaagtagcc gactatcaca gcggctgcta ccaaactcca ctggctatgg gctaacacaa
      961 gtagatagac agataaggat gaacaaatta aaaacaaaga aaaggctagt ttaggcccca
     1021 atttatccat gattatgcca aaagtcatca tccccaaaga catgcctaaa atagtactga
     1081 ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg attttgcata attgaaggca
     1141 gccaattcat taacccaaaa tagccggcaa tttggacagt taccatgata ctaaggccac
     1201 atgtttgcca agcaagatgt tttgattcaa acagtctgct aagtttggtt ttcttttcgg
     1261 cagattgtac agatttgaat tcctttggtt cagtcaagcc gcgacgaatg aacaagacca
     1321 tcaacaccgg cagtacacct acgacataca acatcttcca accaaacgtt ggaatgatca
     1381 cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc accaatacca gcaaccgaag
     1441 aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat caaggacata cacgccccgt
     1501 attctcctcc cgctcctaca cctgcgataa agcgaaagag ataaaccaag tagatgtttg
     1561 aagcaaaata catcaataaa gaagcaatcg aaaacagaag gaccgtttga gaaaatactt
     1621 tcacacgtcc atatttatca gctatgatgc caaaaaaaat acctccaatg agcatcccca
     1681 aattagtgat cgttgaaatg aaacccgctt gcgtaccact aatatgaaaa gaagtaatca
     1741 ttgaagataa tgaaaaagct aaaaacataa tatccatatt ctctaaagca attcccgccg
     1801 atgtcgataa taataccttt cgctgatacg ttgtcatttt tatacccctt ttcctaaaat
     1861 ttccctaaat tgttaaaagt atatccaaaa aagaataaaa aaacaagact tttttatttg
     1921 aaataataca caaacaaccg atatagataa tccagcttag ttttaataga aatgagtatt
     1981 gacttggagt acacttaaag ttgtagaata aataaaatac ataagtaaag tggcaagaaa
     2041 ttaagagaaa gagtgatcag atgaatatca aacaagtcag tgaagaaaag ggcatttcgg
     2101 ccgatacatt gcgctattat gaacgaatcg ggttgattcc accagttaat cggacaaaag
     2161 gcggaatccg tgattataca gaagaagacc taaaatgggt cgactttacg atctgtatgc
     2221 gaaatgccgg attatcgatc gagtctttga ttgaatatat tcgcctatct agcgaaggag
     2281 aagcaactgt atttgagcgg cgtaagcttt tgattgaaga aagtgaactt ttgacgaaag
     2341 aaattgctga aatgcaagag tgtttggaac gtttacaagg aaaaatcaag aaatatgatc
     2401 gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa aaatcaagga gcatcgctat
     2461 ggagttacgc ttactaaaat atttttggac aattgcagaa gaaggaacaa tctctcaagc
     2521 cgcagaagtt ctacatctta cacaaccaac cttaagccga caactgaagg agttagaaga
     2581 ggaactagga acagaactgt ttgtacgcga aaaccgcaaa atgatcttga cagaagcagg
     2641 ttattttcta aaaaagccga gcagaagaaa ttttagattt aacgaatcaa accactcaag
     2701 aattcgagaa cagaaaaaaa caattattca gcggccgatt tacgatcggc tgtgtggaag
     2761 ctgataattc gaatacattg gcactattac tagaaaagtt gatccgtgat tatcccagcg
     2821 ttcaatttca tatccacagc ggaacaagtg atgatatcac ggagtgacta gaaaaaagcc
     2881 tgttagattt agcgattttg ctagatccag tagatacaga gaaatacgaa acgattttgt
     2941 taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa ttctgtttta gcaaaaaaag
     3001 acgtgataac aaaagaagac tttgcgaatg tacctctttt gatctcaaag cgtccagaaa
     3061 tacagcgaat gtttgctgac tggttaggca taaaaaagga gcagttgctg atcacaggaa
     3121 cgtacaattt gattttcaat gtatttgcgc taattgagaa tcaggtaggt gctgctttag
     3181 tcatagaagg tgctgtaagt catctttcaa agcgagtgac attcgttccg cttgacccaa
     3241 tgattgaaac gaactgcatg ctagtctgga aaaagaatcg gattttatca cctgtagtca
     3301 atgaattgat tcaccgtttc aaagaagctt ttgaacagta ataagtatct ctaatcgaag
     3361 aaatttcaat acaaaagcga gacgtatgac agtcttgctt ttgtattttt tcatttttaa
     3421 atcaaaaaaa ataaagagat ttacataact tgttaaataa gcgcttacaa acataagaag
     3481 aacggaaatg aatcagctat aataaccgtt gttctggatt gtaatcatta caaagaaatg
     3541 aagattataa ctttggaggt gttcgaaatg aaagaaaaaa agcaaaaatt cgttcaaaaa
     3601 acatgccctt tattcaatgg ttgggtatga ttctgctgat tgtattagct ttattgacga
     3661 tcattccttt tttatggatg gtttctgctt cattcaaagc aaataatgaa gtgttcacga
     3721 ttccgattca atggatccct aaaacttggc atttagagaa ttatctggtt atttggtctc
     3781 gtattccttt agtgacattt tttaagaata cagctttttt aagcgttgtg atcacgttga
     3841 ttcagttgtt tacttctagt tttgctgctt acgggttttc taaactaaat tttaaagggc
     3901 gcgatatttt atttttagca tatatcggca cgattgctgt cccttggcag tcttatatga
     3961 tcccgcagtt cattatgatg agaaaagtgg gtttaacata tacgttgtgg tcgttggtgc
     4021 tattacaagc gttcaatgct tttggtgtgt tcttgttgaa gcaatactac agtagtatcc
     4081 cagattcttt atgtgagagt gcgcggatcg atggcttatc agagtgggga atctatcgca
     4141 agatcatttt acctcttaca aaaccggcga ttgccagttt gacgatcatc acattcgtta
     4201 atacatggaa cgattatatg gggccattca tttatttgtc ttctacggaa aataaaacaa
     4261 tccagttagg gttgaaaatg tttattgggc tatttgatgc agagtacgca ttaatcatgg
     4321 ctgcctcagt tgtttctgtt cttcctgttg cttttatatt tttagcgatg caaaaatatt
     4381 ttgtagaagg gatcgcttca tcaggaatga aggaatagga ggtgattatg aaagagcagc
     4441 gttttgacaa aaatgtttat atggaattct tttattggtt gtatcaatca ttggcaagta
     4501 gcctttgttt ttggtgtgtc aatatcttgt atatagcagc tgcgttcctt ttgtcgatag
     4561 atggaaggaa tgtcttttcc tttatttttt ctttactttt tattggtcca ggttgcatcg
     4621 tgatgatagg tatgacagaa gaattagcaa aagaaaaaag tttttcacca gtacgtttgt
     4681 tcataaagac gtttcgccaa ttttggctaa agggattttt ttattggcta tttgcttgga
     4741 tagttagtgt gataatgatt tttgattgtt tcttttttat acgtttttct tatggaaaat
     4801 ggttgattcc gttgtttgtt ttacttgctt gtttatcagt ttcctttagc atcaactact
     4861 ggtattttca ggtgagaaat ccagcgtcga aaccaaatca agttctacgg atagcgttct
     4921 actatacgtt gaaaaaatgg tatgtgagtt tactagattt tttactattg acaagtttgt
     4981 ttctcttctt tttcgtaaag ccccaatggt gtattctact aggtccttct atcgtatttg
     5041 gtttgattta cttcaacaat cgtaaattga tgagaactat ggatctgtag actttataaa
     5101 ttgaaacttt caattaagaa tgacagtttt
//
LOCUS       DAJSVD010000149         5110 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSVD010000149 DAJSVD010000000
VERSION     DAJSVD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.152"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1172..1837)
                     /locus_tag="ON498_002642"
     CDS             complement(1172..1837)
                     /locus_tag="ON498_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940857.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2017..3999
                     /locus_tag="ON498_002643"
     CDS             2017..3999
                     /locus_tag="ON498_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR3940858.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4003..4641
                     /locus_tag="ON498_002644"
     CDS             4003..4641
                     /locus_tag="ON498_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940859.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4669..5097
                     /locus_tag="ON498_002645"
     CDS             4669..5097
                     /locus_tag="ON498_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940860.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1682 a          780 c          942 g         1706 t
ORIGIN      
        1 catatgagct aattttatga ttaaatggta agtgtttcct tgtttgtttt agtttaaact
       61 agtgtgtaat ttaaaaatct atatggaagg gtctgtgggg taatacttaa ataaaggcta
      121 ggaacctgat aacatcaagg ttcctagcct ttttataata aaatgaagcg aaaataattt
      181 cttttaaaga gccgagatta tcagctatat aagagtgaat ttgatgcaaa aaacagagcc
      241 gaaacagtct tggtgttcgt agctctgctt atatgtttcg aatttaagac aaagagttgg
      301 caaaagatgt tgataaggaa gataagataa ctgtgttata taaactaggt aactaactgt
      361 aacaagctaa ttttaagaga ttcttattag aagacgttta ggaatataac taagaaaatg
      421 attatacagg taattgtcat ttcaataatg ttttctaatt ttcgcgtctt tgttttaaga
      481 aaaaggccag aatcagttgg aaaaaagttc gtacgatagt gaagaggggc aaaatttcct
      541 actaatattc ccactaacaa gagataaaac ggccaggttg gtgtgattcc tctataaaaa
      601 aatgcccatg ctaccgaaat cccatagatt aacaactgaa caatttttac aatgaaaaaa
      661 ttgttctgcc attactcaaa gtctcttcct ccttgtatcg tttatttgtt tcattttatc
      721 attatgatag gcgcttgaac aatccatttt gtattttatt gagtactctt ggataaacta
      781 ccagcgatta tcatatttat ttatgttact gtaagccatc atataaaagt aaatgagcag
      841 atcatagaaa tataaaaaag aactaaaatt gatctaaatt atgtgcagct tgactacgta
      901 ccaatctata ttacttacta gattggtcgt taatctattc ggatcgaatg agcaaaatat
      961 ttttgtgaat gtccatcctt gattatgatt ccaaaaagaa tcattgggag gctgtggcgt
     1021 aaatattata ctataagcca gaaataccta tcattctgaa cttttttatt taggaacttt
     1081 catctcaagg ctttttcaat taatttgtac ccctgttttt tggataagat catgtgcatt
     1141 gattttttct atctctacgt tcatctccta cttaatgcct atctggctcc tgagggcttc
     1201 tttttaaggc gtcgtcatag tcttggctga ggatttcttg gtaatctgag ggaatggctt
     1261 cccaagtgag caggcgaacg ttcatgacgt gttttccttt gtgtttgatt acagcatggt
     1321 ttttctcttt ttctacctct ccagcgtgaa aagacagcca tgtaccatgg ccaaagatat
     1381 cattacatac ccaacgttcg atggaattgt ccacgtatcc tacagggagt gtggtatagc
     1441 cgatttgtgg tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg tattgataat
     1501 tgataagaaa aagatagacc aaacaaaaga cgagcagaaa gaaagatagt gtctttttga
     1561 gtgagaaggg ttgttttgat gaataagatt tcttgttaac aagaggttca tctggcaatg
     1621 ttggttctat tgattcggta gctgattcaa tagaaggaaa atcaatcggc agacctaaag
     1681 tatgatgtaa aagtgacagc atttcaaggt ctggcattgt acgagaattt tcccaactag
     1741 aaatcgtttt atcggaaaca ccaatttttt cgcctaattc tttttgcgta agcttacaat
     1801 ttctgcggta agttcgtatt ttttctccgg tgttcatctt cttctccttt cgattctaca
     1861 gtatatagat ttattctaca tgacctatac acttgataga tacgtttttt tgatgaaaaa
     1921 cggtatgatt ttatcaaaga atcatttaag taagggagag tggtgataat caaaaaaaag
     1981 ttcacagttt tactcacaat catagtgacc ggtatcatga gtggttttgc cttggttagt
     2041 ataaataaac aggtactcta tcggacaaat gaatttttcc atcaaacaga tacttatact
     2101 ccgattcgat tgccacaaca ttttattggg caagaagaat atgagcagct aatcatggta
     2161 tttgaaaatg tctcatcaga aactgagctt tcttatatga aaaaaacagt aaatgaatcc
     2221 aatgaagtag atacatcttg gaacttcaag atacagccaa agcttgaaat gacattgtat
     2281 tatcaagggg atgtcaaatc ttctcacgag aaggtaccct attctaatac ctcctttcgc
     2341 gtaacgtcca ttgaagaatc gatcaaaata aaagattttg aaggtgaatt tttcttgatg
     2401 acgagagaaa aagaagccta tgaacggttc atttcactat tcactgatcg attcaatcaa
     2461 gcatttcaca ctgattatgc cactaatatt tttgaagact ttgaaaatag ctattcgtat
     2521 tcggttattt caaatacaga cgtatataat tatcgagtgt atttaactat tggattgatt
     2581 cttttattcc ttttgttatc atcctttttc ttattgatga ataaagaaat cagtttattg
     2641 ttgaataatg acttttcatt acatcaagta ttgtggtgtt tattggggaa aaagaaaatc
     2701 attattacat tcataattgc aggaatcatt ggtggtttta tttttatgta ttataaggag
     2761 caatttcttt atttgttcca gatgtacgtt ttcttgttca gcggaatata tggtatctct
     2821 ttgatttctc ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt caactatatc
     2881 caatacattc tttttacatt tggattgttg ttttctcttc ttttgaccgt tgccaatgtc
     2941 gaattagcaa ctatcgtttt ttcttcttca cagttagctg tttcccatga accaccaaaa
     3001 gaaatcgctg gtgaggacta tcatgtattt tatccagtaa ccatcgggaa aaaccacgtg
     3061 gattttattt attccaatcg ttttgcatca gcagcagatc aggaattata tgaaactttg
     3121 aataaaaacg gaagtatatt agtcaatgta tcagattact tgaatgaaga aaatgaacag
     3181 tttggcagag ggataaaaat aaatctgaat tatcttaaga aatttccttt gattgatgtc
     3241 tctggaaggt tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt gatccctgaa
     3301 cgatatagac atacagattt aaaaaatgac ctggcgcaaa taacagaata ttatcaagaa
     3361 atcagtgaaa aagaaccgaa atttctattt ataaaaaaca atcaacccat ttatacattt
     3421 attccttcta tcccctggat cgaacattat ccggttgttg aaatactgac attgaaaaac
     3481 agtatgtatt tggaaagaaa tattctcagc ggagaaatct atccaccgtt aaagatcaag
     3541 ataggctctt tgtcgaaaga aaggttattt gagctgattg aagaaagtca gttacgtgat
     3601 aacttgcagt tatcatttcc gtatactcag actgaagaga tagctgtcaa agtgttaagc
     3661 agatcatttc attacttgat acagatattt ttactcactt tcttttcttt cttcttgttg
     3721 agctatgtca tgttatgcat atattttgtc tacaattgtc ggaaaatcgc tatctttcgt
     3781 tcaagtggtt attcgctctt tgaaacgtat aaagatttct tcatgatgaa cctcatcaaa
     3841 tggggaacga caagtgtaat cttccttttt cttatagaaa gagagcctaa atatcttttt
     3901 aatatttttt tctttagctt cattgatttt attctttcag ttgtttttat tttgtctaca
     3961 gaaaagaaaa gtcagttatt gctgatgaat ggaggttaac aattggagcc gttgatagtg
     4021 ataaaaggtg cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga tcgtttcact
     4081 gcggatgctg gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa aagtacgtta
     4141 ttgaatatat taggattgaa tgatcagttc agttcaggac aattctattt gttcgatcaa
     4201 gagacgaaac aattaactgg gagagagaag cgcttgataa aaagaaatac catatctttt
     4261 ctttttcaag attttgggtt gattgaatat gaaacaatcg attttaattt agatattgga
     4321 ttgaagtttt cgaatttaaa tgtttcagat aagcgaacag caaagtatga ggcattgaaa
     4381 aaagtaaata taaacaagaa gctaagtacg cctatctaca cattatctgg tggtgagaag
     4441 caaagaatcg cctgtgcgag agtcttattg aagaatagca aaatcatttt agccgatgaa
     4501 ccgactgggt cattggataa aagaaataag gataaagtag ttgaaatctt atcgcaacaa
     4561 gcttcccaag gtacgttagt tatcgtagtc acgcatgatc catatctgac caaaatagga
     4621 aacaagaaga tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat gaaaaaagca
     4681 gttattattt gtgcactagg gataggtttt gttggaggaa acgctatgtc atctgaagtg
     4741 gaagcaaatt cagttcacgg tgaaatctat tacaaatctt taaagccagg agagattcat
     4801 tttgatgcta aaggtaatat tattaagtca aatttaccag tagaccggga aatttctaga
     4861 acagcaatta atcatagctc tggaagatgg gtttattata gtgatacaac ttgtacgaga
     4921 aaaacaggtc attctaacca ctatagtcgt gtctataatc gtcacggtgc aaaagcgaaa
     4981 gttggaaact ctattcgatc agcaaatgca acaaaaggag tatgggccta tgcaagtgcg
     5041 acaggaaata gaactgacac attctcatgt tggtacaatc caaccggttg gtattaagta
     5101 tccaaaagtt
//
LOCUS       DAJSVD010000150         5025 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSVD010000150 DAJSVD010000000
VERSION     DAJSVD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.153"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(488..862)
                     /locus_tag="ON498_002646"
     CDS             complement(488..862)
                     /locus_tag="ON498_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3940861.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(885..2285)
                     /locus_tag="ON498_002647"
     CDS             complement(885..2285)
                     /locus_tag="ON498_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR3940862.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2289..2699)
                     /locus_tag="ON498_002648"
     CDS             complement(2289..2699)
                     /locus_tag="ON498_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3940863.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2717..3208)
                     /locus_tag="ON498_002649"
     CDS             complement(2717..3208)
                     /locus_tag="ON498_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3940864.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3218..4009)
                     /locus_tag="ON498_002650"
     CDS             complement(3218..4009)
                     /locus_tag="ON498_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3940865.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(4002..4775)
                     /locus_tag="ON498_002651"
     CDS             complement(4002..4775)
                     /locus_tag="ON498_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3940866.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1593 a         1020 c          673 g         1739 t
ORIGIN      
        1 gaaaacctcc agacttcttg atagctcaag tatatcttga ggtgcatttg ttagtaaggt
       61 actgttctat ggggcgctta ctctaggagt gagcattttt cgaaaaatac taaaaatttt
      121 tcaaaataaa aaaaggcata cagacaataa actgtctata cgcctcacag actctattct
      181 atacatctaa ttttggctgt cctgcaaaaa atatgtgtta gcctccatcg aaagatcaaa
      241 ctttggaaaa catcattata actttgcttg gtaagagctt tgctaaaaca tgatatgact
      301 tgtatacaaa atgtggagtg tattccaata aataggaagg attacaaaat ttgaaaccaa
      361 ataagtaaaa aatactgtca ctttgaatta atttgaaata gtaaattctc attaaactat
      421 tgaaatgctg ataccaaaaa atgaagtact tttcgttcat accaacttag agcacttcgt
      481 tatctattta tttcttcatt tggcttatct ctataatctc atgatttggt ccgtagaaat
      541 taaagtaacg gattccattt tcccagaacg gtaattccat aatattttta tctaataatt
      601 catatcctaa atcttgaatt gaattgaata cctcatctat gtcatccgta tctaatgcta
      661 gatgatcaat ggctcctcta cactcagtag ttttatcttc ccatatttct attatcgttc
      721 cattcaactc gaaaaagccg actttacttc ccacattcat ttcttcatta atcagactaa
      781 accctaagga ttcataaaag atttttgaag ctccaaaatc ttttgttgga attccaatat
      841 gttgtaaacc atttaatttc acttacatca tcccttcaat tgttttaaaa tccacattca
      901 taatttattg aatagtctag tttatctacc ttgattgctc cctccgtaac agtcatactt
      961 aatgctgttc cgactttatc atacattcta gtacttaaag caacattatc aatatataaa
     1021 acagctatcg tatcatcaac aattaacttt agttcatatt ctttgcctat ttccaaaaat
     1081 agtggtcgtt gcagcccctt atcaaacatt tgaggccaag gatagttcgg agtttttccg
     1141 aaagtaagct gattattaat caatgaaata cgaaattcgt atccttcttg agtatcacta
     1201 tctacaaata attttaaagc aaactccttt gttaacgatg taatagtcaa agttgtttca
     1261 aatagaaaat aatcttcgat tttaggaaca attactcttt ctttttttcc aaattctttc
     1321 tctaacttaa aacttgaaaa tggagtgatt tctttaaaat tctcaattat tttttcaggc
     1381 agtttaacac caagagtttt gtctgtcttt tgaataattt gatgtggaac aagtgcaccg
     1441 ccccaatccc agttattttg atcgttctcc ccttcttttg ttgccaccca gccagttaga
     1501 aaacggccag tttcattagt tccgactgtt cttgctgcat aataggcttt tccgtcgaaa
     1561 gcttcgtcta ctggtgattt ccaatctgaa aataatcctg ctccgactcg atatttagtc
     1621 tttttatctt cacaatactc tgaaaaaagt aaataccacg ttccatcaat ttcaaatata
     1681 tcaggcattt caatcatgtt aaattcatta ttagcccagt agtctccttt gaaattccaa
     1741 tcttttaaat cttcggattc aaaatagacc aatctccccg tcggcttttt cttacttgct
     1801 ggttttctag ctcctagtat caaaacgtat tttccatgat cttgatcaaa aattgcaaaa
     1861 ggatcgcgcc aatcattctt atcatatcct tcttgaggaa caagttttgc tgcttcgcca
     1921 tcctttttcc aatgaattaa atctttagaa cttgcaatca ttaacacttc tgaagttttt
     1981 ccatccaact tagctttgcg attatgtcca gtatacaaag ctaaatactc attgattcct
     2041 tggacaatac ttccagcgta aatgtattga tcaatgtcat cgttgccacc acgatgaatt
     2101 acttccccgt attcttcaaa tttcacaaaa tcagtggttg taactagtga ccacccaaaa
     2161 ggatcagtta aaggtttagg atttctcgta tctcgttgat gaaatagata aaaagttcca
     2221 tctttataaa aaggcatgca atccccaaac caaaaatctt taggcttgta aaaaattttt
     2281 tgcatttatc actcatcctc ttcgttagta tgtctttgca tatctgcaag acacgcaatt
     2341 gtattcatat ttcttagatt ttcgatgata tcatcgcttt tttttccttg taatctcaaa
     2401 ttgacagcct caatcaaaat tcctaaattc atgccaccaa gaagttgaaa cccattttga
     2461 aatacttcac ttcttttagc aaattcgttg aaaggtgttc caccaatcaa gtcagcaaat
     2521 accataactt cttgattttt tacttgagaa actatttttt caatgttttc acggaattct
     2581 tcaatgcctg catctccaag tgttaccaca tgacaattta aattatcgcc aaaaaacatt
     2641 tttaatgtat ctacaaatcc aatggcaata tttccatgag atgtaattat aaactccatc
     2701 cactcatcct ctgttcttaa taaataaaat cctttaaaaa ttcttttgaa tctctaggag
     2761 tttgtatcac tgtcacttca actccatcac taattagttc tttaaaagcc tctctctctt
     2821 cttcagtaat atttatcgtc ggacggatgc gttctgttcc attccttctt gataaatttc
     2881 ctacgtttac acttgttatt ggtaaaccgt tatcaatcaa aaatttaaca tcgattggag
     2941 aaactgcaac aataagaact ttttgtcctt catatttacc agaaagaata tttttaactg
     3001 ctacttccct tgtaattatt gaagttctaa cattaggtgg tgcagcgagg cgaactacag
     3061 ctttttttac gtcatcattg gctacttcat tattaataac cataattctg ttagcactca
     3121 attgatttgt ccattgtgtt gctacttgtc catgaatcat tcgatcatca atacgaacat
     3181 gtataatacc tttcattttt tcatccatat tcaacaccta cttcaaaata tttaacgctc
     3241 ttaacgcaat agaaactact aagattaata gaattacata aactggttta acctttcgtc
     3301 ctaacaaata atacacaatt gctactacac atgcaggtaa taaaccaggc ataacttgat
     3361 caaagatttc atttccggtc attgttaagt cgccttgttt aaattcaaag ccaacattca
     3421 cttttacaac tgtggcaatg agtgcaccga ccaccattaa ccctaaaatt gatactgcat
     3481 cagtaataga ctttaatttt gtttcaattt gagttagtag gctcgcaccc gattctttcc
     3541 cagcaaggta taaaggataa gcagtaatcc ttaataaaat tgatgccagt atccacaaaa
     3601 taattccaac aaaactacct tccaatgcca tattagctgc aattgctcca aaaatagtcc
     3661 atggaataac tacaaataca ctatctccaa ttcccgctaa aggacccatt agacctgttt
     3721 tcagagcact tactgcttcc aaagacttat ttttttcttc tttttcaatt ccaacattca
     3781 taccaataat gtaaggcgct gtatacggcg tagtgttaaa aaattgtagg tgagctaaaa
     3841 cggattgttt cattccttca ggatcatctt tatatgtgtc cttgataaaa ggaagcatag
     3901 aatacgcata acccaaccct tccatttttt cataatttaa tgaagttgca ccattgagaa
     3961 tccaccggaa aattacttgt ttgtcgattt ttgtcagttt cttattcatc ttcgtcaaaa
     4021 cctcctgttg cagctggtgc tacttgagta ttatttgatc tatttttata tacgattagt
     4081 gcgattgcag cgccaagtaa cgaaatccct aaaattggta tttggaaata aactgccaaa
     4141 acgaaaccaa ctaacaaata ggcaaagtaa tcttttgtag gtaaatattt aagtagcatt
     4201 cctatcccaa cagcaggtaa tagtccccct gctaccttta aaccaccggt aacccattca
     4261 ggaatataat tgattgcact tcccactaac gttgggccta atatcacagt taacaaaaca
     4321 ggaattccag atacaaagct tgtattaatt gatgccaaat attgcatcca ctctaatttt
     4381 tgataatctt ctttttgtgc gaatttttct gccttttgtt gaaaaaaaat gtttgttgac
     4441 cactttagaa catctagttg tacaattaac attgctaccg gtattgctaa agttattcct
     4501 atagaagcat cctggttagt tgagattgct aaataagtac cgatcaatgc tgctgtttga
     4561 taatccggta ctgatgcacc accaaaacta gtaataccta gtgtcattaa ttgcaaagtc
     4621 ccaccgatat aaagtccagt ttgaatatct cccataataa gtccggtcaa gaaacctgca
     4681 acgacaggtt gggaggttcc gattttgggc ccttccttat cccagttaat gaaaaatcca
     4741 tacaacacaa ctaaaaatgt ttgccaaaac gccattttgt tctctccttt cttcttatac
     4801 ttttatataa gcaattactg tgccaactaa cattatccaa taaagcggga aaatacagta
     4861 aatattaatt aaatgagcgt ctaaatatac tttggacacc ctttcatttt tttagacgct
     4921 agtatgctat ttagaaaata gtctgctcaa aaattgtata gattaactca tactctcctt
     4981 tgggtatttc aatacagtaa atatctttga tttctgaaaa aaaat
//
LOCUS       DAJSVD010000151         4978 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSVD010000151 DAJSVD010000000
VERSION     DAJSVD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.154"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..471
                     /locus_tag="ON498_002652"
     CDS             <1..471
                     /locus_tag="ON498_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR3940867.1"
                     /translation="AKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETKSLFL
                     KDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQ
                     IPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            508..1518
                     /locus_tag="ON498_002653"
     CDS             508..1518
                     /locus_tag="ON498_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR3940868.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1633..2346)
                     /locus_tag="ON498_002654"
     CDS             complement(1633..2346)
                     /locus_tag="ON498_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940869.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2536..3126)
                     /locus_tag="ON498_002655"
     CDS             complement(2536..3126)
                     /locus_tag="ON498_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3940870.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3610..3837
                     /locus_tag="ON498_002656"
     CDS             3610..3837
                     /locus_tag="ON498_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3940871.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3853..>4978
                     /locus_tag="ON498_002657"
     CDS             3853..>4978
                     /locus_tag="ON498_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR3940872.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTF"
BASE COUNT         1684 a          825 c          994 g         1475 t
ORIGIN      
        1 gcaaagaaat tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat
       61 acaaatgaag atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa
      121 agcttattct taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa
      181 caaacggatt tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca
      241 gaacgcatgg aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa
      301 aaacagattc cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca
      361 ccaggtagac cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag
      421 ctacttgaga tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa
      481 atagtgcata cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca
      541 acgattgggg ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa
      601 tcagctttaa agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg
      661 acgcaagata aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg
      721 atgcaagatg aaaatatcca aatgatcttt ccgacttggg gaggagaatt attgattgag
      781 attctcgatt ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat
      841 attagtacgt tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaact
      901 aaccttattg atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta
      961 ctgaaaacaa gagaaggaga atcgattcta cagcaatctt ctgaaaagta tcaaaaagtg
     1021 tggccccatg agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa
     1081 tcagtcagcg gagatgcagt aaggatgaag ggacgattat taggtggttg tattgatacg
     1141 atcaggcatt taatcggtac tccttatgga gatgtccaat cttttaaaag aaagtataat
     1201 gatggaaagc cgatcttgtg gtttctagaa aactgtgagc tatcgtcagt tgatttaaga
     1261 agatcacttg ttcagatgaa actggcaggc tggtttgatg gcagctcggg gatcatgttc
     1321 ggtagaagtg cggcagagga tttgttagat tataaaatga aggatgtata cgaagatctg
     1381 tcaaaggaat taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa
     1441 ctgacattga ttaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt
     1501 ttgcaaacgt ttatttaaaa aattttttcc tgccttggag tctatcaaag accaataaat
     1561 ctaaatagaa aagaaagtga aagaagctga caaaatgctg tcagcttctt tttgtataac
     1621 atattagaaa gtctaattat ctataactgt cacatcgatt aactgtttca cgtcattggg
     1681 atttcctgta ggaatagcta ctacagatgc attatcaatg atttcttgag ttgtttctct
     1741 aatgaacgct ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata
     1801 actagtgatt gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt
     1861 cccagtgagc ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat
     1921 aggtgttcct gcaggataat gaataacatg taattcgaaa tcacccgatg tatctctaaa
     1981 agtattacct ctaatagcta gccaaacacg tgcgtactca atttgtagat cagaatatcc
     2041 tgctaacgga tctattgact gagaagactg tgaggaggtt gtgtctattg ttgaagaaga
     2101 aatatttgtc tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact
     2161 gcttgttgaa gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc
     2221 tgaactggtt tgtctgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt
     2281 actaggagac gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt
     2341 tttcatataa aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg
     2401 aatccgtaaa accttgtcta tgaatataaa tagagtgtat cttctttgat aaatgtcaga
     2461 aaaagtgtga caaataatca tcagtaaaat tacttgtcac actattgata aggagtattt
     2521 aattgaagag aatgattagg agaaaggatc tccgtacttt ttatagtaat catagaatcc
     2581 aagtttgtat agacgaatcg ttttttctct tatttcttct gtttcaaaat ggactgcttg
     2641 aagaatcaag tctgtgaatt ctataggagc tgttccattc gcagtaatca aattacgatc
     2701 tttcacagct tgttgttcta taaaatcttc tttattctga taattttccg attctaacca
     2761 aggaaattgc gcatttccgg tgtgtttgta accttgcaat aatccgctct gagctaaata
     2821 atcaactgcc ccacaaatag cagctactgg tacgtctgtt tttaacaatt tatcgattat
     2881 ttgatataaa tgatgatttt ttatgtccca tgaattgcca ccgatcataa taaaaaggtc
     2941 aactttttca ggtatttgtt cgattaagta atcgattttt gtttttaatc ctccaatgga
     3001 agtgacctct gattggatag aagcagtttt gttttgccat ttctcatttt ggtttagtgt
     3061 gctagtaata taagcacctt cccaatcggc atattcctct aatagaaacg taatcgcagt
     3121 tttcattttc aagctctcct ttactatcta taacaaggac aacagtttta acctacagac
     3181 attcattaaa attgggtagc gacagcgaaa acaacaattc ttaagtttta gacagagtat
     3241 acagatagtc aacttaatta aatacgaact aaataaaaat gacaggtaat catttttgtt
     3301 taccagcatt ataacatttt gcatacatgg atgacaaaag aaccaattcc cacttgcttg
     3361 agtacaatat taaaagaaac tggaaggcaa aaagtaatta tagaaacgaa aaataaatca
     3421 tatcgggcac tcagacatga aaacaaccct agagatatat aactaggtaa ccagtttaat
     3481 gaaagaaaaa atcatccaag aagtcgattc ttggattttt tagtggattt gttcgccaaa
     3541 tacgtttagt atgcgtatat gagaggttgt aagcaaagcg ttgtggtctg agcacttaag
     3601 gaggaatctg tgaaaatagc ttttcatatt tttgcagatt ttgacttaat gccgaaggtc
     3661 agctttgcga ttaccgttta tcgagaggct gtaaaagcac cgtgtagctc cgatgacaat
     3721 aagcaagcat ttttagagct aggtttcctt gattctaaat atgcggctta tttcaatgag
     3781 ttggtgcttg aacgcgattt taagaaggtc gaaaacaggc acgacttttc gacgtaagga
     3841 gaaagaattt caatgataga aacaataaat cccgcagaaa acaaggtttc tgcgtacatt
     3901 catatcccat tttgcgagca tatctgctat tactgtgatt ttaataaagt ttttttagaa
     3961 gggcagccag tagatgagta tgtggaaatg ctgctgaatg aaatgaaaat aatgctagaa
     4021 agtcatccga cagcgaattt agaaacactt tacgtagggg gcgggacacc aacttcttta
     4081 tctgctaaac aacttgatcg actgttatca ggtgctagag agatcctgcc ttttcaaaat
     4141 ggtaatgagt ttactgtaga agcgaatcca ggtgatttga cccgtgaaaa acttcaagta
     4201 atgaaaaact atggcgtcaa tcgtttatcc atgggagtgc agacatttga taatcgctta
     4261 ttgaaaaaaa ttggacgtaa acacaccgca gaagatgtgt atcaaactat gcaattttta
     4321 gaagcagaaa attttaccaa tgtaagcatc gatttgattt atgcattacc gggacaaaca
     4381 ctagaaggat atctggatac tttggaaaaa gctttggctt tggatctgcc tcactattct
     4441 ttgtactcat tgatcctcga aaacaaaaca atgttcatga actgggtacg acaaggaaga
     4501 ctcgaactgc ctgatcagga aacggaaacc agaatgtttg aagagacgat tgaagcaatg
     4561 gaaaagcatg gacgtcacca atatgaaata agtaactttg ggctctccgg acatgaatct
     4621 caacataacc tcatgtactg gaacaatgac cactattttg ggtttggagc tggtgctagc
     4681 ggttatcttg gagatattcg ttaccgcaac aaaggaccaa tccaacacta tttacgccca
     4741 ttacgagcaa atgaacgccc ggtattggaa gaagaaaaat tgacacgaaa aaatcaaata
     4801 gaagaagaaa tgttcttagg actaagaaaa aaaacaggga ttactaaaaa acatttctat
     4861 gatcgctatc aaaaaacaat tgaatcggtg tacggtgatg tgctaaggga attagagcaa
     4921 gaagggttac ttgttaatga agaagaccgt gtttatttga caccaagagg gacctttt
//
LOCUS       DAJSVD010000152         4915 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSVD010000152 DAJSVD010000000
VERSION     DAJSVD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.155"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            81..380
                     /locus_tag="ON498_002658"
     CDS             81..380
                     /locus_tag="ON498_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3940873.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            645..1520
                     /locus_tag="ON498_002659"
     CDS             645..1520
                     /locus_tag="ON498_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940874.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1517..1735
                     /locus_tag="ON498_002660"
     CDS             1517..1735
                     /locus_tag="ON498_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940875.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1722..4349
                     /locus_tag="ON498_002661"
     CDS             1722..4349
                     /locus_tag="ON498_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940876.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            4364..4582
                     /locus_tag="ON498_002662"
     CDS             4364..4582
                     /locus_tag="ON498_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940877.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         1915 a          611 c          984 g         1405 t
ORIGIN      
        1 ttttttacgc aatttgtatt tatttgtact tataaaggta gaggagttat agaataatta
       61 attaaaaagg agaggttttt gtggaagcaa taaaattaat gatttcagaa gaacaggagg
      121 aaatgtttct aaagcagttt tatcaaattg ctacaaaagc tattgaaaag gctcaagaaa
      181 atgcggggat gacaagggaa tatctcaatc aaaaagaaat ggcggaatac ttaaatgtga
      241 gtgaaaactt tttaaaaaat gctgtgagaa atgagggatt gcccgtagtt gaattagggg
      301 caagaaaatt ttattcgaaa aaggcagtta atgagtttat gtttgctcga atgaaaagag
      361 gggaaaatat tggaagatga tgaagtagaa attataaaaa aatgctttaa aaaaagaaaa
      421 aaagtgttcc caaaagtttg cagactgggg gaacactcaa taataaagtg ctgagaaaac
      481 tttatttata gctattatat caaaaaagaa ggtgaaaagg tgttttcttc tggaaatgtt
      541 gtagttgaca aacttagaga aataaatttt gacggaaatg taatccacac acatggtacg
      601 atcaattaag aaaaaaaacg aaaaaaggtg ttgagaagcc ctatttgaat gcaatagtta
      661 tcttagcaga aattacctat tggtatagac ctaaaaaaat ttttaacgat gatggtcagc
      721 tacttggtta tcaaaaaaag tttcttgaag atattttgca aaaatcatat aagcaattaa
      781 gcaaaaaaac aggattatca gaacgagcta taaaagatgc aattgtgtac ttagagaaaa
      841 aggggattat caaaagggtt tttagaaata tttattatga gaaaagagca gttacattat
      901 caaatgttat gtacattgtt ttaaatgtac ataaattgat agaaattact tttcctactt
      961 tggattgcga aaaaaaggag tatatgccga aaattcgtca gaggggtatc gaaaaaaaga
     1021 gatatgtctc tttaaaaaaa ggtagaggta tgtacaaatt tgtatatacg tatacaaaga
     1081 ctacttcaca gaacatatca aagactactc tcgttatgga agaagaggat attaatataa
     1141 agaggattta cagtaagatt agtgttcttt atgacgcaaa taacgaagat ggtcgaaaat
     1201 tatttcaagt gtatcactat ttgagaaacg ctaatgtaga tacagaagat atcagaaaaa
     1261 tcattgaata tttatctgaa aatactcagt atcttattgc tgagtgtatt ataaaacaac
     1321 atgaagcatg cttttttcaa agaaaaactg aagaagggtt atacgattat gtaacatatt
     1381 ttttgaatgg acttcagaaa agatataaaa gtcaattttt agaaaatgta gagcctatag
     1441 atcagttcaa aaaagctttc gaaatcaaag agttacctga aattactatg cataattggt
     1501 tgaaaggaga agaatcatga ttaaaaatcg cttaatagat caaatgtggt gtcttgatca
     1561 aaatacagat actctcgaat taatcgagag tatctgcttc aacacagtag ttctcgattt
     1621 aagagctgaa aatgacaatt gtgtaactca tatcataatt aatcaaaaaa tggctcagca
     1681 gctctctgag agcctaagaa aatgggctga aggagaaaac tatgaaagca attaaagaaa
     1741 aaatcgttag tagaaaaata aactggttag cactcagtgt acttgtttta atgattagcg
     1801 ttgtttttac aagtaatatt gttgaagcta gcattttcaa ttggtttgat gacacaaaaa
     1861 gtcaaacaga gtttttgaat aattctcttt atagacctta cctaaaaaaa tacaatggag
     1921 atttccttag tcagttcggg atttggcttg gatgggctgt ggtaaaaggc atgtttactg
     1981 tgactgattc gattcaaaat atgattccag atgtgttgga tttgttcaat ttcattgaaa
     2041 gtacagggct aaataatgtt tatcaatcag tcataaatac aattgtcgtt ggattaatga
     2101 tcctttcatt gatgtttgtt ggctacaaga tgattactgg aaaaggaaca atagatttaa
     2161 gatctgttgg aatgaatata gtgatgtctg tagcgttaat tcttttaatg ccaacaatga
     2221 tttctagcgg tattcaattt gccaaaatat tttacaatga tgctactacg attacaaatt
     2281 cagatgacgg agtcgcttgg tcattgataa aacaaggagt aacagatctg gcatatatta
     2341 ataaaacaga tcaatactca agcattgata aaacggagga tcgaaataag ttaacacgaa
     2401 aaaattttca acagacagat ttaacacaag tgttgacgga taaagtgatt gataaattgg
     2461 aaaaagaaaa tccagctgcg gataatcttc gctatgaatt agttgaaaat tctaataatg
     2521 aatttgttgc cacaaagttt tctgataact tcttatcaac attttctgat agcttgaaat
     2581 caggttatta tcgatatcaa gcaaatcttt gggggatttc gattgggttg acagcgttgg
     2641 caatcgccta tgtgttcagc gcgtttgtga tcattacagc aatattagaa cttgcattta
     2701 aacgtgtgtt aggtgttctt gtttttgcga cagatattga aacaggacaa cgttcaaaag
     2761 ttgttttatc cgatatttta caatgttact taacagttgg atttcaaggg tttggactat
     2821 ctatgtttgc tatgtttatc aattttttaa atgctggaca aggtatctcc acaaatattt
     2881 ttataaaaac aattgcttat atctgtgctg tgtttgtgct gataaaaggc agtggaacag
     2941 tcatgagata ctttggtgtt gatattggac tcaaagaagg ctatgggcaa cttgcttctg
     3001 cttttggtat gggcgcaatg ctgtttagaa aaggatcaac tggatttaat cgtgctaaag
     3061 gaagcggaaa tggaaatgga tcagacagtg gagaaggaga agatcgaaaa ccagaaaaga
     3121 atttcggcga aactctatca aaaaaagctg gcaaaactgg aagagctttg ggatatgccc
     3181 atgaacgtgg attatcaggt ttagcttcag atggagctac aatggctagt gaaagagcaa
     3241 taaaaccatt taagagtatg cgtgatatgg ctaatgatac caagaataag ttcaaagaag
     3301 gtttagatga tggtacagtg tccgctatta ataaaaatag caaacctatg ttagctaaaa
     3361 ataaagaaga tgaaactgga aaatatgctg attccatgcc aacacgtctt tcggatagaa
     3421 aagatggtgc taaggttgaa aatgccgata gaattatgtc tagttctgaa cgtatgcgtg
     3481 aagcaatgaa aaataatgct gaatcgaata ataatcctgt aagtgcaatt cagcaaaaag
     3541 ttcaacaaga tattgaagaa cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag
     3601 aattaattaa tcagaagcga caagaagcta aatacacgcc tgaagcaatg aatcgtgagg
     3661 aaatgttaag gaaacgtgta gaagggcgta caggagctaa tacggacgaa aaagaagcgt
     3721 tgaccaaaga aagaatccaa gaagctaaaa actcaaatac cggattagaa aaattggtca
     3781 aagaaaatct acaaaattct acaagtagtc aaggtggccg atcagtagat gtccgagaaa
     3841 atattcaagg ttcattgaat ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac
     3901 aaagctcttc aaatggtcaa ggtactaaga caattgatgt tcgagaaaat gtacaaaatt
     3961 ctacaagtag tcaagggttt aaatcaatgg atgtaagaga gaatgtacaa aattctacaa
     4021 gtagtcaagg gtttaaatca atggatgtaa gagagaatgt acaaagttct acaagtagtc
     4081 aaggattcaa aacagtagat gttcgggaga acgttcaaaa agatagtgga gaaatgaaag
     4141 aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa
     4201 atcatgaaac agtgattatt gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa
     4261 gacgaagatt tacctatgaa gacaacgagt tattcagaga tacacttaat gatcctaatc
     4321 cgcttttcga taatctatta aaaaaataaa agggggaatt gtcatgaaat taattctaaa
     4381 gattagtacg catgatttct tagaacttga cgaattatgt gaatcagtaa agaacgcaac
     4441 aaatgaaaaa atcacggtca ctaattatat tgaactgatg attcaagaag aattaaagat
     4501 gaaacatgag ataaaaaaca acttgaaata ttgttatcta gaaaaaagtg aagagataat
     4561 ccatgaatta aatggctttt aaaaattgta tttgacatga atttacgaca aggaattagt
     4621 ggggattaag cgattattct cttccacttt agagaaataa tgggttaaaa agccagaata
     4681 cgtagagcaa tattgacaac ctataaagga gctattgttg aaaattgaag taacaaaaag
     4741 ataaagagag gtttacatct atcagattaa aagtgatagt acacccatga aacctgcaaa
     4801 agagttgaac ctaatgtata tcggtttaac tctttttatt tgcaaattaa aaaaatatca
     4861 ccgaagtgac gaatgaaact atgaccaaat taagcaagaa aatatatctt gcaaa
//
LOCUS       DAJSVD010000153         4911 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSVD010000153 DAJSVD010000000
VERSION     DAJSVD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.156"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            699..1520
                     /locus_tag="ON498_002663"
                     /pseudo
     CDS             699..1520
                     /locus_tag="ON498_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            1663..1887
                     /locus_tag="ON498_002664"
     CDS             1663..1887
                     /locus_tag="ON498_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940878.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            1902..2771
                     /locus_tag="ON498_002665"
     CDS             1902..2771
                     /locus_tag="ON498_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3940879.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2752..3486
                     /locus_tag="ON498_002666"
     CDS             2752..3486
                     /locus_tag="ON498_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3940880.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            3519..4427
                     /locus_tag="ON498_002667"
     CDS             3519..4427
                     /locus_tag="ON498_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCR3940881.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4424..4672
                     /locus_tag="ON498_002668"
     CDS             4424..4672
                     /locus_tag="ON498_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HCR3940882.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(4675..>4911)
                     /locus_tag="ON498_002669"
     CDS             complement(4675..>4911)
                     /locus_tag="ON498_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3940883.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1656 a          744 c         1071 g         1440 t
ORIGIN      
        1 ttagtgaata ggagagtaac gttattcaca gtattgggta ctgaaaatta acagcatacc
       61 tcagtttttc aaaaaacagc gaagcctatg gatatgttcc cgtctaccga tagtgcaaaa
      121 agtgtcgtgg atatgtttcc gagaacggac atacacaaat gacattcatt ctgtcctctc
      181 aatccatgtg gaagcagtat gtctgttaac aagaaaataa ttattgttcg tcctgacgag
      241 ttgttagact cgaaaaggat aaacatcatt tttagaaccg gtagccgatt tttggctagc
      301 ggttttttgg gctctttggc aaatagtggt gattgaatct cgcgctatcc tatggaaaac
      361 cgccttgtgt gaggggctgc gagaccgctt cgatcctaga attgctgaag cacgaatcta
      421 gcgtcactca catttcatct tttgacgtag ctgagacaag gtgtttttct tcgatccgct
      481 agtggttaat ctctttttgg tgcaaatatg gtttagaatt tctttgcatg gcttaaggga
      541 gtggagaatc ggatattagt aggatttaca tccacagata aaaataggct tgattttaac
      601 agcgaaacta aaatacccta cttaaatgaa gtgatagatt agatgttggg ttgttaggag
      661 atgcttaaaa atcctatgca atcaatagtt tttagtgttt gttgacttga tccctaaatc
      721 gcctgagtac taaacgttga gtgtgtctgc ttactgtcac tagccagtaa gtgagactgt
      781 agttaatctc tcattttccc cagtattatg ttgaaaatag ccatgaagcg attatttcta
      841 aagagttatt tttgcaggct caagaagaaa tttatcggag aagtaatatc tacacaggag
      901 aaggcaagaa caaaagaatt tatagtagca agtacgcttt aagtgccatc accttctgtg
      961 gatattgtgg cgatatttat aggagaacct attggaatat tcatggcaga aaagaatttg
     1021 tctggcgatg cgtgactaga atcgagcagg gtcccgaggt atgtaagaac cgaaccgtaa
     1081 aagaagatga actctatggt gcggtaatga ccgcaattaa taagctactt gctggtggca
     1141 acaatatgat aaagacactg gaagaaaata ttcatgctgt gattggagaa accacagaat
     1201 accaaatttc agagattaat accctactag atgaaaaaca aaaagaacta atcaagctgg
     1261 ccaataaagg tcaagattat gaatacctgg tagatgagat cgatgagatg cgagataagc
     1321 gtcagactct tttagtagaa gatgcctccc tcagtggcga gaacgagcgc atcaatgagt
     1381 tgattgaatt tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa
     1441 ggaagataat ccagaacgtt aaggtctatg aagaccactt cgtcatagcc ttcaaaccag
     1501 gtatcgaaat ggaaatatga aaaccagaag taaatagccc atgactctga agtagagttg
     1561 tgggttttct tttaattgct gattataata atattttaat gactattgca tttatcaacc
     1621 aaatggttta tgttattctt agcataaact tggaggtgct gtatgactac ggcagaaatg
     1681 attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc
     1741 cagactccac agaattttaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg
     1801 aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga
     1861 gatgaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc
     1921 aaatagtaac agagaaatta tcatctttgc cttttataga aggcattgta ttagggggct
     1981 cacgtgcaag aagcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt
     2041 cagactcatt tgacctgaca gcgattaatc aaattgctac agagttggat gatgagaata
     2101 gaaacaacct tgttgtacct cccggagcgt ggggtgattg ggttaatggc ggtggatggt
     2161 tagttataaa cgggtgtcat gttgacttga tattacgtga tataaaacgg gtggaacaaa
     2221 taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg
     2281 gttatataag tgctatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga
     2341 acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa
     2401 gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg
     2461 cgggggcaga ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa
     2521 atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac
     2581 tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg
     2641 aagtactcgg tctttctctt tttgaatgct acgacatgac cgagaaactt tataaagaag
     2701 tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa
     2761 aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa
     2821 ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt
     2881 aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac
     2941 ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga
     3001 aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca
     3061 gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag
     3121 aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt
     3181 gaacatcctg tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa
     3241 atactgcatt tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg
     3301 gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca
     3361 aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt
     3421 ccggggatgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag
     3481 atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga
     3541 tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg
     3601 ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat attacatatt
     3661 ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata
     3721 taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt
     3781 cctatcttat gctatttgat gattacaata aaattgatct taccttattg cccttggaag
     3841 agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta
     3901 gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa
     3961 gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat
     4021 tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc
     4081 tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca
     4141 agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca
     4201 cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt
     4261 tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata
     4321 ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc
     4381 tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa
     4441 agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat
     4501 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     4561 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     4621 tattgacaat actgataaga taatatatct tttaacccgt ggtgctagtt aattttaata
     4681 tcctaaagaa tatctcaaag ttcctccaaa acgtcgttgt gctcgactta atagaacact
     4741 gtaagccagt aatatgcttt caaatctact ctctagccct ttaacagaac gtgagttgaa
     4801 attttgacaa cctaattttt ctaaggaaga aaaaacagtc tcaactgttt ttctttgttt
     4861 tccaagtaag ctgtggtcta gtttttctga tttatccatg tttttacgtg g
//
LOCUS       DAJSVD010000154         4861 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSVD010000154 DAJSVD010000000
VERSION     DAJSVD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.157"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(112..1260)
                     /gene="nagA"
                     /locus_tag="ON498_002670"
     CDS             complement(112..1260)
                     /gene="nagA"
                     /locus_tag="ON498_002670"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3940884.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1336..2172)
                     /locus_tag="ON498_002671"
     CDS             complement(1336..2172)
                     /locus_tag="ON498_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940885.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2356..3573)
                     /locus_tag="ON498_002672"
     CDS             complement(2356..3573)
                     /locus_tag="ON498_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3940886.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3722..3901)
                     /locus_tag="ON498_002673"
     CDS             complement(3722..3901)
                     /locus_tag="ON498_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940887.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3956..4039
                     /locus_tag="ON498_002674"
     tRNA            3956..4039
                     /locus_tag="ON498_002674"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3990..3992,aa:Leu,seq:gag)
     gene            complement(4279..4857)
                     /locus_tag="ON498_002675"
     CDS             complement(4279..4857)
                     /locus_tag="ON498_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940888.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1485 a         1006 c          783 g         1587 t
ORIGIN      
        1 aggaaattaa agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa
       61 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
      121 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      181 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      241 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      301 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      361 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      421 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      481 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      541 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      601 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      661 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      721 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      781 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      841 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      901 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      961 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
     1021 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1081 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1141 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1201 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1261 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1321 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1381 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1441 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1501 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1561 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1621 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1681 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1741 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1801 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1861 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1921 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1981 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     2041 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2101 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2161 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2221 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2281 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2341 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2401 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2461 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2521 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2581 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2641 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2701 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2761 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2821 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2881 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2941 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     3001 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3061 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3121 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3181 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3241 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3301 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3361 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3421 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3481 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3541 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3601 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3661 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3721 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3781 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3841 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3901 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3961 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     4021 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4081 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4141 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4201 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4261 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4321 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4381 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4441 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4501 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4561 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4621 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4681 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4741 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4801 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4861 t
//
LOCUS       DAJSVD010000155         4845 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSVD010000155 DAJSVD010000000
VERSION     DAJSVD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.158"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..132)
                     /locus_tag="ON498_002676"
     CDS             complement(<1..132)
                     /locus_tag="ON498_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3940889.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..2914
                     /locus_tag="ON498_002677"
     CDS             542..2914
                     /locus_tag="ON498_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940890.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2975..4435
                     /locus_tag="ON498_002678"
     CDS             2975..4435
                     /locus_tag="ON498_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3940891.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            4446..>4845
                     /locus_tag="ON498_002679"
     CDS             4446..>4845
                     /locus_tag="ON498_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HCR3940892.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFK"
BASE COUNT         1855 a          786 c         1032 g         1172 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaaga
     1081 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
     1141 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcgcc
     1201 aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat tcaacgaaaa
     1261 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga
     1321 tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata aattctattt
     1381 tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag gggatggcgt
     1441 caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag tacctgaaaa
     1501 ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc agtcgaaaga
     1561 aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta agcaagaagt
     1621 caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt ctcctgaaga
     1681 cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta gaaatcaatt
     1741 attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg cgttgaaaaa
     1801 agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt
     1861 caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga
     1921 taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga
     1981 aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc
     2041 gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat
     2101 tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta
     2161 tcaaagagaa ttaggacaag cacggggggc gtatgttcgt aacgaattta ctggcgaaga
     2221 agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa gtattcatga
     2281 attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta aagaattgca
     2341 ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt cggagaaagc
     2401 tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata aggaagcggc
     2461 ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca ttcaaaccat
     2521 ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag cagaagtgaa
     2581 gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc ccgttggttc
     2641 actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc gaacgttaca
     2701 tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt taaaaggaaa
     2761 aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc taggaaatcc
     2821 attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg aaatctatta
     2881 tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac tatctgaaaa
     2941 acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa tgagtcttat
     3001 tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa tggctgtagt
     3061 caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg atcgaagaaa
     3121 acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat tagctaaact
     3181 ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag ccacagattt
     3241 tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg agacagaaaa
     3301 gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga attatctgaa
     3361 ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag ggttgattgc
     3421 cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt cgagacgatc gtttagatca
     3481 tgcggtcgcc tttttatgga aagatccaca gacaaatgag acagtagggg cgagttatca
     3541 aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc atatagataa
     3601 aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc agttaaaatt
     3661 ttttgagagt agtattgatt tacttagcta tgctgcacta aatcgtgatc agttaaacga
     3721 tacgtggtta gtttcgatgg aagggttaaa acaccatgtg atcagtcatt attttggtga
     3781 ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga tttgtgtgga
     3841 taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg cagttgatcc
     3901 atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc aagtgccaaa
     3961 agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa aagatcacac cagaagctat
     4021 tatggccata cacaagacag aaaacaatct gcaattaacg aaccaattgg tttccgctca
     4081 caaagtaaat gcgtcttttg gtcagcagct ttctgttaac gattcaatag aagctattaa
     4141 tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gcttgtgaac gcgtggatgg
     4201 tacgtacgat tttgatcgtt tttatcaaga aaaaggggat attaatgctc agattctttt
     4261 ctcttataaa gcagaacaat actacaaagg ctataaaaat catgaacatg aatttgtacc
     4321 tgaagtaaaa aaggattgga atgatcaatt gaagcatgaa atctaccaac aagagatcag
     4381 aaagcaaaaa cgtgccatgt tgtttcaaca aggtagacag caagaaaggg agtgaaatcg
     4441 tcatgatgaa aaccgttatt ttagcagaaa aaccgtcaca agcgaaggct tatgccgata
     4501 gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga ttgttttcag
     4561 gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg cccgatatgt
     4621 acgatgaaaa ttggaaacaa tggtcattag agcatttgcc tattttccct acacattatc
     4681 actatcatgt tcctaaagat aagaaaaagc aattcaaggt ggtgaaacag caacttcaat
     4741 cggccgacac cattattatt gcgacagata gcgatcgtga aggggaactg atagcttgga
     4801 cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaa
//
LOCUS       DAJSVD010000156         4832 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSVD010000156 DAJSVD010000000
VERSION     DAJSVD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.159"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(177..437)
                     /locus_tag="ON498_002680"
     CDS             complement(177..437)
                     /locus_tag="ON498_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940893.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            512..715
                     /locus_tag="ON498_002681"
     CDS             512..715
                     /locus_tag="ON498_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3940894.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(856..1341)
                     /gene="ssb"
                     /locus_tag="ON498_002682"
     CDS             complement(856..1341)
                     /gene="ssb"
                     /locus_tag="ON498_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3940895.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1344..2243)
                     /locus_tag="ON498_002683"
     CDS             complement(1344..2243)
                     /locus_tag="ON498_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3940896.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2256..2849)
                     /locus_tag="ON498_002684"
     CDS             complement(2256..2849)
                     /locus_tag="ON498_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3940897.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2925..3074)
                     /locus_tag="ON498_002685"
     CDS             complement(2925..3074)
                     /locus_tag="ON498_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940898.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(3090..>4832)
                     /locus_tag="ON498_002686"
     CDS             complement(3090..>4832)
                     /locus_tag="ON498_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3940899.1"
                     /translation="AEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSL
                     GRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFED
                     YLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQG
                     LYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKV
                     QEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQL
                     QLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQP
                     YTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKL
                     VVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLE
                     VVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDF
                     KLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEK
                     KKTNYKKRTRRTTK"
BASE COUNT         1205 a          973 c          848 g         1806 t
ORIGIN      
        1 atatcttagt gaacgtgcaa cgttcacttc ctttcttctc gaacggcggt ggcggttgga
       61 gtgagagaaa ggtttttcaa ttgagaaggc acttgtgtct tcctaaaaag gtcttttcct
      121 gtttctatgt aaaacaaaaa gagctgcaat cctcatttag gactgcagct cttcttttat
      181 atgcccatcg atcgcgtaaa atgatcgggg tttctaattt gcatcgattc aatgactaat
      241 tgattccgtt tattgaaatg acctgtgacg gcaatattgt attttccatc atccataatt
      301 aacaatttat cgataatttc aatatctgca actacacaat taatcgattc atgaattgtt
      361 tgtaaagtaa agcgaaccaa taatggttgt gtttttatta ctcggatttt atcaagcaaa
      421 ccaacatatt tgttcatcac gatcacctcg ctaaaattat agcaaaaaca gctaaaattg
      481 agaattttta ttcgatttta gatatgctat aatgatcaag acagtaaagt ttgtgacggt
      541 ggctatccga ttgaaagtga ggtggtgcgt atgtgcgtac ttccaaaatc ttacagaaag
      601 gagaagcctt ttgcctgttg aaacggcgct aggattaatg attagtttcg gttctttgat
      661 cgcaacaatc atttttggaa tcttagcagt tgtaaacaac aatcaaaaaa aataaccgtc
      721 agctttagcc ggtcgacgat tatttttcaa aaataatatt aagctggtca ccgtcttaaa
      781 cggtgctgtt aggggagttg ttagcgcaac tcctcttttc gtatccatta taacaacttt
      841 attgatcttt tacaatcaga atggtaaatc atcatcacta atatcgattg attgtccgcc
      901 aaatggattt tgtgaagcgt tcaatccatt gttgttaggc gtagctacat cccgttgttc
      961 aaaggtggta gggcttggac tgctggacga ttgatcagcg tgcgcacggc tttctgtcgc
     1021 tgctttactt tctaatagtt ggaaattatc ggccacaact tctgtcacat acacacgttg
     1081 tccttgttgg ttatcgtagg ttcgggtttg aatccgtcca acaacgccga tcaaactgcc
     1141 ctttttagct aatgtcgcca tcgtttctgc tggtttgcgc caaatcacac agttaataaa
     1201 atcagcttct cgattgccgt tttgattcgt gaaatttcgg ttgacagcta gggtaaaagt
     1261 cgctactgca gtaccactcg cggtataacg taaatctgga tctttggtta agcgtcctac
     1321 taaagttaca ttattgatca tctttatctc tccatttctt gattcatttg ttgtgatctt
     1381 ttaaactgcc gttttctttc ttgattttca attttgatca cacttttcat taggtgatca
     1441 atttcgttga ctgtatctgt aaatgaaatg gaattcgcaa acgtgttaac tgagcgatat
     1501 ttttcccact gcttttccaa atagtcattt cctctgatct tatccatttc tctttcataa
     1561 taaaaggaca acggttttgt atccccacgt tttgtaaatg tatttcgaac acttttgtat
     1621 aactctttcg tatcgatatt ttctagtttc ggaatggtaa ataagtcgta gtaatctttt
     1681 gctctcgaat tatctgtgcc aaagctgaac gtagcactta gcttttctgc aataatttgt
     1741 tctgttggat aagctggaat ctccactttt ttatcttcaa acattaattg gtgatagtgt
     1801 gtatctactt caggataaat agaatctcct gttgtcacgt ccattttaaa cggtacacgt
     1861 actttctcta aattagccac caatttaatt tgaaatccag gataaaaatc ggcttctctt
     1921 gtttccttca ttccttgaat agagaaaaca atgccttctt ttgtgggttt tctagttaaa
     1981 tcattaaaaa tgctcttcaa tttttcttca gttaatttgc tatttctaaa tgtcgtatcg
     2041 atatcaattg ttgtccttct atcgattcca tattttgatc ctaataaaaa gccgcctttc
     2101 aaaatgaagt catttttgta ctttgattca gaaatttgtt caattagacg atccatcata
     2161 aaatgccgtt gcactaactg tgcatttaat cctgtactct ttgctaaatt tcttgttttt
     2221 gctgcaaatt gtgtcggtgt catctttttc ctccatcaat ataagctttg catgtggtat
     2281 ttcatttctt tttctacatg tagttttgtc atgtaatctc ttaatttact tggatctctc
     2341 aatgggtctt ccatgtactc ttttaatgct tctaatttca tttcatattc aaattcatat
     2401 ctcggattcc aaatatcaca aagtgtacgc tctttatcat acatagtaat aggatttcct
     2461 tgaaaggaag ttccctctac ttttcctagt tcataaactt ctttcttcac gaaatgaaat
     2521 tgtaactgct gctcttttgt aattgaacga tcaacgttat agccatgggg aaaagttaag
     2581 tgaatgaatt tcggcatatt cgtagaaagc tggtaaatat ctaaagtcgt ttcatgagag
     2641 aagattcccc tggaaaatcg ttgctgaagc caaaataaat catctattcc atgaccatcc
     2701 tctacataat aaccacgatc aactttgatc aattttcctt gattcaccaa tctttgtaca
     2761 gtggttgctg cgattccaaa tttcctggct tccttcatag ataaatttcc atcgcgaata
     2821 gccaacaatt ctttcagttt atcttgcatt tacttcccct cccttcgtta caaatacact
     2881 acatcaaata tatcatagta gtttatttgt aacaagatta acgtctatcg atctaattcg
     2941 atttcttttt gaatgcgctg ctggttcttt aattgttgca gtcttctttt atttgcacgt
     3001 aatttctctt gttctaaacg aatttttgct ttttgatttt cgatttcttg ttgtttgtgt
     3061 gttgtatttc gcataggata accccttcat tattttgtag tgcggcgtgt tcttttctta
     3121 tagttcgttt ttttcttttc agaaaattca aagcctgttg taaagtcttc ttttaaaacg
     3181 atcgttgctt caaacgaacc tttctgacct tttagtcctc tgactgattg gcttgttcgg
     3241 ccgttttgaa tgatctcttt cagttgtgtg gctgtgagtt tctttttggc aatcgttgtc
     3301 cataatttaa aatcacaggc ttcagacgta catgccgcca cattttgata aagcaagact
     3361 tcttgttccc gacattttgg acaaacacct aaatgacgcg ttgcttcctg tttttcactg
     3421 gctgctttca cctcttgtaa cgttgatcca aaatctgttg actggatttg tcccggtacg
     3481 acttccaaca agtgagagac aaatttttgg atattagcta agaaagtagc ttgtgttcct
     3541 ttctgctcac caatctttaa taagtaactt tcccatttgg cggtcatttc ggcacttgtt
     3601 agcagcggct ctttttccac ggcttggcaa agtaaaatcc ctttatttgt caccacaagc
     3661 ttattttttt ctaccttgat atattccttt tgtttcaaag tttcaataat atttgctcgt
     3721 gttgcttctg taccgattcc ttctacttct ttcaatatgg attgtgcttc ttcgctatcc
     3781 acggtttttc cagctgtttt catcgctgtg atcaacgtac cttcggtata aggttgtggt
     3841 ggcttcgttt ctttttcaag gacttgtaga tctaccgtca ccgcttctcc ttttgtcact
     3901 ttaggaagta gcggttcttc ttccttttct tttctgtcgc tctctttgcc aaacagcttt
     3961 ttccaaccta gatctactgg tacttttccg actgtcttta gctgaagctg ctgaacttgc
     4021 gtcaagatcg ttgtttcttc ataggtatac ttttccgcaa acatggccac cgttgttttt
     4081 aacacttgtt cataaatagc ctgctgcagt ggacttaatt gttcaaaagc cgctgccgtc
     4141 atgaccgtct ttgtaggaat gatcgcgtaa tgttcctgga ctttcttatt attcacatat
     4201 cgactattcg gtgtatgaat aggagtagga atggcttcgg cctttaaaaa gtgtttgtat
     4261 tcgtctaagt gatccaacag atacgcatac tccccttctg taatataagg ggtatctgtt
     4321 cgtgggtagc ttagatattt cccttcatac aacccttgca tggcttccaa agtatctttg
     4381 gcactagctt tcatgagctg attcatttta gattgaaggc tggatagaga aaaaagacgt
     4441 ggacttgccg cacttttttt ctcggtttca acttggtgga tcgtacctgg ctgttttccc
     4501 aattgaaccc ccttttcttt taaataatct tcaaatgctt cttgcgtcgc ttgggtttca
     4561 ttcggatcaa gcttcgcatc aaatgcgcca ttttgagcga ttacttgggc ttttccttca
     4621 aaatatggtt ctttttggaa gtttctaatc ttttcctgta attgataaat catgtaaagc
     4681 gttggggttt ggactctgcc taaggaaaaa cttcctggta tgcctttttg ctgcaagagt
     4741 aaactataca gcgggctacc gttcatgccg atcaaccaat cggcgatctg gcgtgcttgt
     4801 gcttcttcaa attttgggta ggtctcttct gc
//
LOCUS       DAJSVD010000157         4795 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSVD010000157 DAJSVD010000000
VERSION     DAJSVD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.160"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            21..716
                     /gene="vanR"
                     /locus_tag="ON498_002687"
     CDS             21..716
                     /gene="vanR"
                     /locus_tag="ON498_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3940900.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            694..1848
                     /locus_tag="ON498_002688"
     CDS             694..1848
                     /locus_tag="ON498_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3940901.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2063..3031
                     /locus_tag="ON498_002689"
     CDS             2063..3031
                     /locus_tag="ON498_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3940902.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3024..4055
                     /gene="vanA"
                     /locus_tag="ON498_002690"
     CDS             3024..4055
                     /gene="vanA"
                     /locus_tag="ON498_002690"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3940903.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4061..4669
                     /locus_tag="ON498_002691"
     CDS             4061..4669
                     /locus_tag="ON498_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR3940904.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1478 a          918 c         1129 g         1270 t
ORIGIN      
        1 tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat gaacatgaaa
       61 ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc aaatactata
      121 ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc atattggaca
      181 tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac aagcacacct
      241 atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca gggttaacaa
      301 tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt gctcgggtaa
      361 aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta
      421 tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg aacgagaagc
      481 agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa aacaagggga
      541 atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat ttcagcaaga
      601 gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac gacaccattg
      661 ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa aaataaaaaa
      721 aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat tgttgtggta
      781 gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg ggattggatc
      841 ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa attatatcaa
      901 tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat tagtattctt
      961 attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat aaataccggc
     1021 attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga aatggatgtt
     1081 atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca ggatgcaaag
     1141 ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat taaaacgccc
     1201 cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat gccggtagat
     1261 caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga acagctaatc
     1321 gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac aaaaacgcac
     1381 atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca gctttccgca
     1441 catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg cgaccctgat
     1501 aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag tgaggataac
     1561 agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga attcaagaac
     1621 actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta taggctggac
     1681 aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc aaaagaaatt
     1741 attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac gacgtttagg
     1801 gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga gatgtatata
     1861 attttttagg aaaatctcaa ggttatcttt actttttctt aggaaattaa caatttaata
     1921 ttaagaaacg gctcgttctt acacggtaga cttaataccg taagaacgag ccgttttcgt
     1981 tcttcagaga aagatttgac aagattacca ttggcatccc cgttttattt ggtgcctttc
     2041 acagaaaggg ttggtcttaa ttatgaataa catcggcatt actgtttatg gatgtgagca
     2101 ggatgaggca gatgcattcc atgctctttc gcctcgcttt ggcgttatgg caacgataat
     2161 taacgccaac gtgtcggaat ccaacgccaa atccgcgcct ttcaatcaat gtatcagtgt
     2221 gggacataaa tcagagattt ccgcctctat tcttcttgcg ctgaagagag ccggtgtgaa
     2281 atatatttct acccgaagca tcggctgcaa tcatatagat acaactgctg ctaagagaat
     2341 gggcatcact gtcgacaatg tggcgtactc gccggatagc gttgccgatt atactatgat
     2401 gctaattctt atggcagtac gcaacgtaaa atcgattgtg cgctctgtgg aaaaacatga
     2461 tttcaggttg gacagcgacc gtggcaaggt actcagcgac atgacagttg gtgtggtggg
     2521 aacgggccag ataggcaaag cggttattga gcggctgcga ggatttggat gtaaagtgtt
     2581 ggcttatagt cgcagccgaa gtatagaggt aaactatgta ccgtttgatg agttgctgca
     2641 aaatagcgat atcgttacgc ttcatgtgcc gctcaatacg gatacgcact atattatcag
     2701 ccacgaacaa atacagagaa tgaagcaagg agcatttctt atcaatactg ggcgcggtcc
     2761 acttgtagat acctatgagt tggttaaagc attagaaaac gggaaactgg gcggtgccgc
     2821 attggatgta ttggaaggag aggaagagtt tttctactct gattgcaccc aaaaaccaat
     2881 tgataatcaa tttttactta aacttcaaag aatgcctaac gtgataatca caccgcatac
     2941 ggcctattat accgagcaag cgttgcgtga taccgttgaa aaaaccatta aaaactgttt
     3001 ggattttgaa aggagacagg agcatgaata gaataaaagt tgcaatactg tttgggggtt
     3061 gctcagagga gcatgacgta tcggtaaaat ctgcaataga gatagccgct aacattaata
     3121 aagaaaaata cgagccgtta tacattggaa ttacgaaatc tggtgtatgg aaaatgtgcg
     3181 aaaaaccttg cgcggaatgg gaaaacgaca attgctattc agctgtactc tcgccggata
     3241 aaaaaatgca cggattactt gttaaaaaga accatgaata tgaaatcaac catgttgatg
     3301 tagcattttc agctttgcat ggcaagtcag gtgaagatgg atccatacaa ggtctgtttg
     3361 aattgtccgg tatccctttt gtaggctgcg atattcaaag ctcagcaatt tgtatggaca
     3421 aatcgttgac atacatcgtt gcgaaaaatg ctgggatagc tactcccgcc ttttgggtta
     3481 ttaataaaga tgataggccg gtggcagcta cgtttaccta tcctgttttt gttaagccgg
     3541 cgcgttcagg ctcatccttc ggtgtgaaaa aagtcaatag cgcggacgaa ttggactacg
     3601 caattgaatc ggcaagacaa tatgacagca aaatcttaat tgagcaggct gtttcgggct
     3661 gtgaggtcgg ttgtgcggta ttgggaaaca gtgccgcgtt agttgttggc gaggtggacc
     3721 aaatcaggct gcagtacgga atctttcgta ttcatcagga agtcgagccg gaaaaaggct
     3781 ctgaaaacgc agttataacc gttcccgcag acctttcagc agaggagcga ggacggatac
     3841 aggaaacggc aaaaaaaata tataaagcgc tcggctgtag aggtctagcc cgtgtggata
     3901 tgtttttaca agataacggc cgcattgtac tgaacgaagt caatactctg cccggtttca
     3961 cgtcatacag tcgttatccc cgtatgatgg ccgctgcagg tattgcactt cccgaactga
     4021 ttgaccgctt gatcgtatta gcgttaaagg ggtgataagc atggaaatag gatttacttt
     4081 tttagatgaa atagtacacg gtgttcgttg ggacgctaaa tatgccactt gggataattt
     4141 caccggaaaa ccggttgacg gttatgaagt aaatcgcatt gtagggacat acgagttggc
     4201 tgaatcgctt ttgaaggcaa aagaactggc tgctacccaa gggtacggat tgcttctatg
     4261 ggacggttac cgtcctaagc gtgctgtaaa ctgttttatg caatgggctg cacagccgga
     4321 aaataacctg acaaaggaaa gttattatcc caatattgac cgaactgaga tgatttcaaa
     4381 aggatacgtg gcttcaaaat caagccatag ccgcggcagt gccattgatc ttacgcttta
     4441 tcgattagac acgggtgagc ttgtaccaat ggggagccga tttgatttta tggatgaacg
     4501 ctctcatcat gcggcaaatg gaatatcatg caatgaagcg caaaatcgca gacgtttgcg
     4561 ctccatcatg gaaaacagtg ggtttgaagc atatagcctc gaatggtggc actatgtatt
     4621 aagagacgaa ccatacccca atagctattt tgatttcccc gttaaataaa cttttaaccg
     4681 ttgcacggac aaactatata agctaactct ttcggcagga aacccgacgt atgtaactgg
     4741 ttcttaggga atttatatat agtagatagt attgaagatg taaggcagag cgata
//
LOCUS       DAJSVD010000158         4661 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSVD010000158 DAJSVD010000000
VERSION     DAJSVD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.161"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            266..2410
                     /locus_tag="ON498_002692"
     CDS             266..2410
                     /locus_tag="ON498_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3940905.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2462..3502)
                     /locus_tag="ON498_002693"
     CDS             complement(2462..3502)
                     /locus_tag="ON498_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3940906.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(3779..>4661)
                     /locus_tag="ON498_002694"
     CDS             complement(3779..>4661)
                     /locus_tag="ON498_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940907.1"
                     /translation="EIADLTENNSGYDLVMNNPRAEDQQKIKELSPTLSASYTQKEDA
                     GTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNELTVQEELRSYELPEQQEK
                     EIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQNKMNNPSLQKVEYSNQK
                     YEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGSKSDIARYEMLEKIGTRR
                     GLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPYKDIWVPFTIFFVLYFIY
                     YVLTTWLYTGIVLKKRL"
BASE COUNT         1459 a          976 c          848 g         1378 t
ORIGIN      
        1 ttccaaactt tggtataatt tacatatgca taaccatttt tatccccact tgtttatccc
       61 caaacaaagg tggtctatgc aataaaatga acgtcaaaga aaaacgaatg aatgctcgtt
      121 gcttatttac aaattgtttt atttagaata cacagacaga tttatgaaaa gaaaaagctt
      181 ttcgacacga ttatctgttc caaaaaatgt ctaatgaatg ctgaataaga cagaaaatca
      241 atgaaaggaa aaggactagt ttattatgaa aaataaaaaa acgatacgtt tgctagctgc
      301 tacttggctg attgctccgt ctgttttgtc gaccaatacc gtatttgccg tagataatca
      361 aatagctgat caaacagtca caacagatac gactacatcc agctcagcta ctagtgaaac
      421 aacggattct tttacagaag acccaactgg aacaactaac tcaacaacag gatcgaccga
      481 gccaactgaa attgatccgg ctgatccatc agaaaatgaa aagccagccg agacatattc
      541 ccttacaatt gctccttctt tgattggtaa gatccaactg actgcttcga cagcggattc
      601 ccctgaacag caacctttgg atattacgga aagtggaacg gtagaaggtt tattagcgga
      661 aacgaaggta acttacacta ttacgccagc tgaaggtgaa caaattgctt cagtcgctgt
      721 taatggtgcc aaagaagtaa attatgatgc aaagaccttt acagtaggta cgaacaattt
      781 atctattact gcagaattta aagctggacc ggatacttca acagagaaac caactgatcc
      841 tagtgaagaa gtccctagta caccagatcc ggaaacgcca aacaaaccag atgtgggtaa
      901 taacaacaat aataatggtt caaataattc aaacaataat aacaataatt cgaacagcgg
      961 gaattcttcg aatcaaacaa acccatctgg atcgaataac aatggaaata ctcagagaga
     1021 accaaaccat ccaaacaatg gttcatcgaa tggcggaacg aacaatgctt catctcgtcc
     1081 aaatacagca aatcaaagcg gtatagagaa tccatcttct gcttcttctg attttgttgt
     1141 aaaatcacca attgatgctg tattgcctac aaatacaacg aatgtccaac aagcaatcgt
     1201 tcgagaagca ttcaagcatc ttggaaaacc atatgtatgg ggagcaaaag gtccaaatac
     1261 ttttgactgt tctggtttga cttattacgt atatatgaaa gcaaccggtc attatatcgg
     1321 tggctggaca ggcgaacagc aatatgcagg tacgcagatt ccagtaagtc aggcccaacc
     1381 tggcgacctc gtcttctggg gaccttcttc cggtgttact caccatgtag gtatctatat
     1441 tgggaacgga caatttatcc atgcgcctca accaggagat aaagttcgag taacttctat
     1501 ttcagatttc acgcctgatt ttgctgtccg agtaaactta gcaggtttac ctgctgcttc
     1561 aggttcgctg acaggcggct catctatttt agatggatta gatggctcgt tccatttcag
     1621 tcaaaaccaa acaactgatc agttcttgaa aaagattgca gatgatgcac aggaaatcgg
     1681 gcaaaaagaa ggaatctacg catcagtcat gatggctcaa gctatccttg aaagtggatc
     1741 tggaaacagc ttattatcaa gcgaaccaaa tcataacctt tttggtatca aaggatcata
     1801 taaaggaagc agcgtgacct tcaatacact tgaacaagat agttccggac aaagctacca
     1861 aatccgcgca caattccgta aatatccttc ttacaaggaa agtttggaag actatgccga
     1921 cttgatcaaa aatggactga ctggcaatcc tgatttctac aaaccaactt ggaagtcgga
     1981 aacaaaagac tacaaagaag caaccaaata tttggaagga cgttatgcta cagatcgcca
     2041 atattcacaa aaattgaacg cgatcatcga agcgtacgac ctgacaaaat atgatgaacc
     2101 gaaaaaagaa gcagcaacaa ctgaagaagc aaacgctgaa atcctcaata tgtctaagcg
     2161 attcgatgtg ccaatcaaat ggacaacaga tggcgtgcca atcacaggat tagaattttc
     2221 aacgataaaa cgcgtcaaca acgcttcttc catcttccgt gtcgcttcaa tctgggattt
     2281 gtggaacaat tttacggcaa gacagatacc agaaacaact actcgtacag ttaaagctgc
     2341 aactgttcct gtactaacct tgttaagttt agacaaatca ttctggcttg atctgccttt
     2401 aaagaaatag aataaattaa aaaggggatg tgacactgtc acactccctt tttaatttat
     2461 tctatttctt tatatttatt ttgtattccg ttacaaactc gcgtacttgg ttaggttcca
     2521 gagtgacatc tccaaaactt tcaagtcgct ccgctcctgg cagttcttga gtctcaaatg
     2581 tgataccgcc atgatttact aatttattcc cgcgcatttc cggtccatct tctccaaaat
     2641 ttgctgtaaa aatcacgatt cctgaagcat ctgtttttac ttggatcgat actttttcat
     2701 ctgggcttgt caattccgca gcatttagta aaccagattg ttttttcaac aaaaatggat
     2761 gatcgatgcc gcccactatc attgattggc gaaaatcact ggcaaagacc tcacctaatt
     2821 ttttaggacg ttggaagtca aatgctgtgc cacttactga aactacttct cctgtaggcg
     2881 ttgtatcttt attgagcgta gcaaataatc ggctattcaa ccaaagtata tgttcgtcga
     2941 ttgactgtgc tggatcaccg gttaaattga aatagacatg atttgtagga ttgaatactg
     3001 ttttgcggtc actgatccct tggaccgtca ctcgccatgt atcatcgttc gtcagcgtat
     3061 accgtacttc tacatctaaa gttccaggaa atccgttttc catatctggg ctagtctgat
     3121 agaaaatgac cgatgcttcg ttttcaccat tgatgactgt ataatgccac tgcttggctt
     3181 caaagctagg tgcaccacca tgtaaacaat gaccagtgtc cgggtctgtt tttacttcga
     3241 acgtttcatc atctaatttg aatgtgccat tcgtaatccg tccagccata cgtccgattg
     3301 aagcaccgat ataagggtct ttttctagat attcttttgc ttcatcaaaa cctaagatca
     3361 actctcgttt ttcattatcg attattgttt gaaaatgaac aacccgtgcc ccaagatcac
     3421 tgacagacaa agatgtccca ttttgattcg tgatcgtaat caagtgatag ttttcgccaa
     3481 aattttgttc attgatttcc atgttccttg caccctcttt tctttcattt gcactgattc
     3541 cagtatacat ggaaaccgtt ttcttaacag cttattccgt gagtaacgag aaaaaattta
     3601 ctatattcta ataaaagacc gtgaaaaaaa ggttgtgaca aaaatattgt cacaacctct
     3661 ttttccgaac aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3721 aaatatgagg agctattttt gcttggctgt tcttattact tgatgcagga cagctttttc
     3781 acaatctctt tttcaatacg attccagtat ataaccatgt cgtcagtaca tagtagatga
     3841 aatacaatac gaagaatatc gtaaacggaa cccaaatatc tttgtatgga ttttgcatga
     3901 atagaccaaa catttgtaat ccgaataaga catggactac acctaagatt cctggtgcta
     3961 agaataacac gcctacttct ctacggatcg attgtttaag caacccgcgt cgtgttccta
     4021 tcttttcaag catttcataa cgagcaatgt cactcttgct tcctgacaag atcttgaaca
     4081 tcaaacagct agcaagcatg gtcaagaaag ctaaacctag gaagaatccc ataaattcaa
     4141 aaccagagaa tagaccatta tacacttcat acatttcata tttctggttg ctgtactcta
     4201 ctttttgcaa agatggattg ttcattttat tttgagcaac aagtgtttcg attggtttca
     4261 ggtttgcttg gaagtctttt acctgaacta gctgtaaagt ggattgtggc aagttcagtt
     4321 gatcaaattc tgattgactg actaccttga tttctttttc ttgctgttcc ggcaattcat
     4381 agcttcgaag ttcctcttgt acagtcagtt cattcatttt tttagcagga actttttctt
     4441 tcgtgatctt cggcggtgtt ccatcttcat gttttatcat gatcaatggt tgagccgcaa
     4501 attcacttgc attatagtaa atcgttcccg catcttcttt ttgagtataa gaagcagaaa
     4561 gtgttggtga aagttctttg attttttgct gatcttctgc ccgaggatta ttcatcacta
     4621 gatcatatcc agaattgttt tctgtcaagt ctgcaatttc a
//
LOCUS       DAJSVD010000159         4594 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSVD010000159 DAJSVD010000000
VERSION     DAJSVD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.162"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..364
                     /locus_tag="ON498_002695"
     CDS             <1..364
                     /locus_tag="ON498_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR3940908.1"
                     /translation="IRKVKGFQQEKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQ
                     LITFNGVFKEYKKKYESGDLDRYKKKDENEYFWFLTASWMRKESKYAVDYREMSADER
                     NYFSGMYVDEKSVEGENE"
     gene            361..780
                     /locus_tag="ON498_002696"
     CDS             361..780
                     /locus_tag="ON498_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940909.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            759..1097
                     /locus_tag="ON498_002697"
     CDS             759..1097
                     /locus_tag="ON498_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940910.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1101..1463
                     /locus_tag="ON498_002698"
     CDS             1101..1463
                     /locus_tag="ON498_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940911.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            1685..3091
                     /locus_tag="ON498_002699"
     CDS             1685..3091
                     /locus_tag="ON498_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR3940912.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            3965..>4594
                     /locus_tag="ON498_002700"
     CDS             3965..>4594
                     /locus_tag="ON498_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR3940913.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDI"
BASE COUNT         1599 a          652 c         1042 g         1301 t
ORIGIN      
        1 tattcgcaag gtcaaagggt tccaacaaga aaaagcagta caggaagttg cgaaatattc
       61 ggcaaaagat tttgaactga ccgaaagtca ggaagtgttt gacacattct atcatgcaat
      121 gaaaggtcgt cagttgatta cgtttaacgg tgtattcaaa gaatacaaga aaaaatatga
      181 gtcgggtgac cttgatcgtt ataagaaaaa agatgagaat gaatattttt ggtttttgac
      241 tgcttcgtgg atgaggaaag aatcgaaata tgcggttgat tatcgtgaga tgagtgctga
      301 tgaaagaaat tatttttcag gaatgtatgt cgatgaaaaa tctgttgagg gggaaaatga
      361 atgactgatt caacggataa aatgacaatt aaggcgcttg ctgatgaact aggaataacc
      421 aaaaatacag tgagaaatta tctagtcaaa gccggatatg atcttgctcg gtacaagaaa
      481 aatggaatta tcttgctaga caaagagttg attggtgttg ctcggtcaat ttatcggtca
      541 aaaggtggtc aattgactgg tcatttaccg gtcaatagca cgggcagtga ttttcctttg
      601 gaaaaagaga atgagttttt gcgtgatcaa ttagctgaga aggacaaaca tatttctgaa
      661 ttaacagaaa tgattcgtga tcaacaaaaa ttgaccctgc ttcagcaaca aaaaaacgat
      721 cagttgttac tagaaaatca agaattaaaa aatcgaaaat ggtggcaatt ctggaagtga
      781 gatgtgacat ggataaaatt gatttgagag aagaaacagt caatagaatt gaagtgatgt
      841 atgaacggtt agctgattat ttgaatcttt cggatgaaga taattatagt gagatgcttg
      901 aacgaatgat ggatgttctc aactcggtta tcaatgctga tctagcaaaa tcttcagttt
      961 tgactaatga atttgttcat aagcatttag cgcaaacaac tgatgaagct gaaaaagaat
     1021 tttggttgaa attaatgagt catattatgc agttaaacca aatttccgtt attaattcca
     1081 aagaaaaggg gatgtagttt atgggaatga ctttagcaga tattaaagtg caagaagaaa
     1141 aattaaaaaa agaacaggcg aaacttcgcc aacagaagaa acgattgatt gagaaagaaa
     1201 atgctgcaat gggcaaggct cttcgcaagc gttttgggtt tgaatctttt gctgattttg
     1261 agcggtggct atcgtcgatt tctgacgatc agcaacacga aaaatcagtg ggtaatgagt
     1321 tgcgagctga tgaaaaatca gcaccaagcg aacggaatta ccctaacgaa tctgacggaa
     1381 tattcggagg gataccgaat agaaaaagtc agatgagtga gagtggattt gcgtcaatgt
     1441 ttagtgacgt aaattcacga taagcgaata ttttgcatga agcaaaataa agcgtaaaag
     1501 gagttgagag cgtgacacgc tttcggctcc ttttttcgtt gtcacttgtg acggcgaaaa
     1561 ccctcggaga gcgtttggaa ttgtgacagt catgtcacaa ttaggaaaac gttactcttt
     1621 gacaaagagt aacagaaagg tttacactct ttttattagc gacagcgcga aggaggggtt
     1681 taacttgggg tttagtattt cgttcaaaaa gggaacaaag aatacaagca tttctcataa
     1741 taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata ttgatttcac
     1801 aaaatcggat gaaaatatct atttggaaca aataaatatt cgtgaaaaat atgaagaatt
     1861 attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata gaaaaattga
     1921 ggactattta gcaaaggttc gcaaggataa aaaaatggaa ccacagcgag aatttatcgt
     1981 gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt ctactggcat
     2041 atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag ttgagtattt
     2101 caaagaattt aaagaacgga atcctagttt atccgtctac aatgctgtta ttcataatga
     2161 cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt ataaacgggg
     2221 tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac gagcagataa
     2281 agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt caattgaacg
     2341 tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa tcaaagatat
     2401 tcatgaatac aaagaaatca tgagcgaaat ttccgaattg cgtcaaacgg ccgtaaaaga
     2461 gcgtgagagc gtttctgatg agttgaggta tatttgtagg caaagagaag aaaacgagcg
     2521 tgagaaggct ctggtggctt ctgagagagc gaagattgaa gaagagaaga aaaatatgct
     2581 gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat atggttcctt tagaaagttt
     2641 caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg atttgtatca
     2701 acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg agcaatattt
     2761 acagatgctt gagcaaaata cagaattaaa tgagtatgta gctgcattgg aagaagagcg
     2821 tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa agcttaaaga
     2881 agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc aaaaatcgat
     2941 cactcgtttg tctatccgtt ttgggcttgc agaaaaggat atggggcttt ctgaggaatt
     3001 agaagtatta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac agcataaatt
     3061 agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg gtaccggagg
     3121 cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa gctgacattt
     3181 gctaaaaaat ggccagtcca ttttttggcc aacttagcgt tagcgaagtt tgggggcgcc
     3241 cttttcaaag ggtgcggttt tatgtttttg gatatttgca tagctaaaga gaagtgctat
     3301 tattgagtta tcaaataaat aaactgaaaa aagagcaaaa aaataaagtc gagcaacgct
     3361 ggacacgttc tcgaccttta cggaaactaa tcacactagc tccgtaactc aaattcatta
     3421 tacgaataat ttcaggtttc ttcaagacta gagattgctg gttatgaatt ttttagagtt
     3481 acgtgaactc tggaaaattc tttccaccaa tctctttttt ctttgaggag gaactaaatt
     3541 tgagtaaaaa aggcacgatc ataaatgagt gggaaaaatt aacagccccc tctaacaggc
     3601 ggttaaatgt aaaaactttt tacaccccct taaaaatgca gtggtcgatt gatcgaattt
     3661 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccctctttt
     3721 ttgatgtttt ttcttatctt gatactatag gcaactattt ctgcgaagaa atcggggcta
     3781 tagtatgggt ctatcccttg tggctctaag gaagaacaaa aaaaaagatt gctttttctt
     3841 ccctagtatt ttatagtttt aattgactag aaaaaaacta aactagaaag gagagcaatc
     3901 tattttttgt tatcggcttt tcagcctttt ttgtatacaa aatttgtaga ttcagtatag
     3961 caagatgaaa aagaaaaagc aagatttgat taacggaaca tttcgagaaa aaaagaaaaa
     4021 tttgaaaaaa gtggtggcat ttagtgataa tcgaatttct cccaaaatgc gtgaattgat
     4081 gacaggctgt ggttcatatt tagaatttat tgcaacgatt gataaggaga aaaagaaatt
     4141 ggtccaagct cacttttgta aaaatcgttt ctgtccgtta tgtgcttggc gtaaagctcg
     4201 taaggattcg atgatgttgt cgattatgat gcaagctatt gcggaagaaa agcagtatca
     4261 atttattttt atgacgttga cgacaccgaa tgtaaaagcg gaatgtttga acgatgaaat
     4321 tgatttgttt aatcatgcat tgaaaaagat gtttatgcgc aaacgggtta agacttcgat
     4381 taaaggctat gtgcgaaaat tggaaatcac gtacaacaaa aaacggaatg actataaccc
     4441 gcattttcac ttgattttgg cagtaaataa atcttatttc acggataaag attattatat
     4501 caagcaatcg gaatggttag atttgtggcg ggaagtgacc ggaaaaacgg gaattaatcc
     4561 ggatggtacc gacgaaatta cccagttgga tatt
//
LOCUS       DAJSVD010000160         4567 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSVD010000160 DAJSVD010000000
VERSION     DAJSVD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.163"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(16..963)
                     /locus_tag="ON498_002701"
     CDS             complement(16..963)
                     /locus_tag="ON498_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3940914.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1141..1944)
                     /locus_tag="ON498_002702"
     CDS             complement(1141..1944)
                     /locus_tag="ON498_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR3940915.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2090..3004
                     /locus_tag="ON498_002703"
     CDS             2090..3004
                     /locus_tag="ON498_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3940916.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3052..3522
                     /locus_tag="ON498_002704"
     CDS             3052..3522
                     /locus_tag="ON498_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR3940917.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3607..4215
                     /locus_tag="ON498_002705"
     CDS             3607..4215
                     /locus_tag="ON498_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR3940918.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGGFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4349..4549
                     /locus_tag="ON498_002706"
     CDS             4349..4549
                     /locus_tag="ON498_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3940919.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1500 a          709 c          903 g         1455 t
ORIGIN      
        1 tattgttttc agctattaat atcctgcaga caattttact tgattggttt gaagctcatg
       61 ggtggcaaag tagcttttca gattggttaa aaaaatagcc attaatttct tttgaaaatc
      121 aggtgtcaaa cctgcaatgt gggaagtaat cagtacattg tccattttcc aaagaggatg
      181 atcttgtgga agaggctctt cctcaaaaac gtctaatgct gcaaaggaaa gttgctggtt
      241 atttaaagcc tgcaccagat cttgtgtctt aacagaagca ccacggccaa cattgatgaa
      301 tacagatttt ggatcaaagt gttcaaacat atgctgatca aagagcccag ttgtttcctc
      361 cgttagaggt aagatattga ctacgaaatc tacttcagga agaacttcct ttagttgatt
      421 taaagaaaaa gtcttttcca ttccttctgc tggatgaccg gatgtattga ttccatagca
      481 gttcatgtct aatgctttgc atttttttgc aatcattttt ccaatatggc cagttccaat
      541 gattagtatt tttttccctt ggatagattg gtaatggacc gatgaaccta accatttcat
      601 gtcactctgt gccttttgtg cttgaaaaag accacgagaa tacccaagga tgattcctaa
      661 gacatgctcc gtaatcgatt cactgtggat gccactacca tttgaaagta aaatatgttt
      721 ctttgaaaaa gtctcaaggg gtagctgatc aacaccagct gagatagatt ggacccattt
      781 caaggtagaa gaggctaata gtttttcttg gtagtctgag ttccagccca aactgatctc
      841 tacattttgt agatcctctg gttgtaattc tgttttaaag atataaccag gcgctatagt
      901 atggatttct tcgataaatt catcacgaaa tggacgatct gcataaatga ttctttctga
      961 catagttaat ttcccgcctt tctttactct aagtgtagcg catacaagaa gaactttgaa
     1021 gcgccttgct tgttttttga gaaagaaata tttttcaaat aaaaaagggg ctgtgacaaa
     1081 aaaatgtcac aacctctaaa agggtctatt gaagtagaaa taacgagttg tctttatttc
     1141 ttaattgatc gatatttttg cccatccttt gacagcttga gcaaaaataa agattcctgc
     1201 aaaagcaatg aaggcgctag tgtcaaaaga aggaagaaag accatcaaga ttgttgcaat
     1261 tatagtaggc aatgaaattg aatagatcaa tgtttttaaa caatcaagaa aagtagcttt
     1321 tgttagtcga aagcgtgcat agagaaaagc agcaatgttg gtaaatagca aagtgatcag
     1381 cagattcaga aaactaggat aaatagaaaa tataaatgtc aggactttta tccaaagtgg
     1441 tatagatgct tcacttaaaa aagaacggag ctgcttacct gttaagtttt ttaaagattc
     1501 attggtgtag ggaagttcta attggttatt gttcaacaaa gcagaagtcg tgcctgtatt
     1561 aggaagagct atgataagtt tgttttttaa catcccgaca ctaacaaaat tccccatcaa
     1621 atcagtagag atgtcctgtt ctgttcgttt tccctctgga tcaaatgtga agataatgga
     1681 gtttgtttgg taaatgaatc cttctttttc ttcggtttga agctgcccgt tttggattgt
     1741 aaaatcagga atgcgagtag caatcttttg cccatccaat tgaatgttgt ttaagacttg
     1801 aaaaacttga aaagagattg gcaaagccat aattatactc aaaactaaaa gatagacaat
     1861 ggctttccaa aatgggacat ttctcgcctc ctttaggtct ttgaatcgta taaaggaact
     1921 gataattaat tgttttgttg tcataagtac tcctcatcta gtagtgtcat cacttgcaat
     1981 tctagcgaag tatcaagtga aatacaagtg ctttaaggaa acagctgaat gattggtgaa
     2041 atatcaaaaa aatgttattc ttgtctaaag actatccaat ataaataaaa tggaggaagg
     2101 aatagtgcct acacagccac agagatcatc aaaaaaacta tttggagtag tggttttgaa
     2161 tgcggtgatc actttatcag aatttattgg agggattatg tcagggagtt tgtctcttgt
     2221 ttcagatgct ttccataacc ttagtgatac attggcaatt attttttctt acggggcaca
     2281 aaaaatggcc cgaaaagaag caaataaaaa acgtacgtat ggctaccagc gtttagaaat
     2341 attgagtgct ttcatcaact cattcatatt gatcatatta tccttgtttt taaccgttga
     2401 agcattcaag cggttcactt caccagaaaa gataaatagt catttgatgc taacagtagc
     2461 agttattgga ttgctcgcga atttgttctc agtattatta ttacgacaag aagcagatga
     2521 aagtttgaat ataaaatcta gttatttgca tttgttaagt gatacactgt cttcggtaag
     2581 tgtgattatc ggagcaattt tgatccgttt tttcgatatc tactggatcg atccagttat
     2641 tacactgatc atttcgatat atatattgat agaagcgata atggtgatca aaaaagctgc
     2701 cgctattttg atacaaagtg cacctgctat cgactatgaa aaaatggaac aagaaattga
     2761 agcaatcgat ggggtaaagg atgttcatca tgtccatatt tggcagtata gcgaaaagat
     2821 aattattttc gacggacata ttgattttga agatcagcta ctaagcgaga ttgaaaagat
     2881 atatcctcgt ataatttctt tattaaaatt aaaatatggt attacacatg tgacgatcca
     2941 agcagagaca aaggcaaagg atcaaaaaaa attaattttt ttaaataaag atgaaagtta
     3001 ttgaaatata gagtatgata aataagaatt tttaataagg taggttggct catgaagcag
     3061 tatcgacagt taaaaggata tttagaagca aaaggatatt gggagatact agtctatctc
     3121 ttttttggtg gtttggctac gattgtaaat tttgttagtt ttgctctcgc acgccaatat
     3181 tttgatttaa gtatggcctt atccaattct atttcgtggt tttgttctgt attgtttgct
     3241 tttgtaacga acaaactttg ggtattccat tcaaaatcac cgaattttac acatgcactt
     3301 attgaatgcg gtaagttctt cttctaccgt atcctttctt acggattgga catgggggca
     3361 atggtattgt tgatcaatgt tatgaacagc aatgagtatg tggcgaaaat catcactcaa
     3421 attattgtta tccttgcgaa ctatattttc agcaagttgt ttatttttaa agaaactgag
     3481 gtttttgaag aagaagtagg tcaaagtgaa aaagacgact aggcataaca attgaagaat
     3541 ttccttctct ttgttactct ttgtaatgta gacattatta agaaaacatt gaggaggaat
     3601 ttttttatga cttacacatt accagatcta ccttatgcat atgacgcatt ggaaccttat
     3661 attgacgaag aaacgatgca tctgcatcat gataagcatc acaatactta tgtgacgaat
     3721 ttaaattcag caattgagaa atacccagaa ttaggcgaaa aaacaataga agaattatta
     3781 tctgatatgg acgctattcc aacagatatc aagacagctg tacgtaacaa tggtggcgga
     3841 catgctaacc attcattttt ctgggaaatc atggcaccaa atgctggtgg cgaacctaca
     3901 ggagaaataa aagaagcgat taatgaagct tttggtggtt tttcttcttt taaagaagaa
     3961 ttcaaaaaag cagccgctgg acgatttggt tctggatggg cttggcttgt aatggaaaat
     4021 ggaaaattag ctattacctc tactgcaaat caagattctc cattgatgga aggaaagaca
     4081 ccaattctag gtttggacgt ttgggaacat gcttattatt tgaaatataa aaatgtccga
     4141 ccagattata ttgcagcatt ctggaatgtt attaattggg atgaagtaaa caaacgtttc
     4201 gaagcagcaa aataaccatt tcctccgtca tatcagtctt aaaaataatt tggaaaataa
     4261 aagagcgaaa gcttgctagc gttttctaaa ttgggtataa tgttcttgca aggttacttg
     4321 caagaacttt tttggaggat atacatttat ggaacacgga acggttaaat ggttcaacaa
     4381 tgaaaaagga tttgggttta ttacagtaga cggcggagat gacgtatttg ttcatttctc
     4441 agcaattcaa ggagacggct tcaagagcct agaagaaggt caagaagtag aattcacgat
     4501 tgttgaaggt gcacgcggac ctcaagctgc tgaagtgact aaattataat aatttgttga
     4561 ataaaga
//
LOCUS       DAJSVD010000161         4560 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSVD010000161 DAJSVD010000000
VERSION     DAJSVD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.164"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..792
                     /locus_tag="ON498_002707"
     CDS             <1..792
                     /locus_tag="ON498_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3940920.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ON498_002708"
     CDS             889..3138
                     /locus_tag="ON498_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3940921.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..4316
                     /locus_tag="ON498_002709"
     CDS             3141..4316
                     /locus_tag="ON498_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3940922.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4319..>4560)
                     /locus_tag="ON498_002710"
     CDS             complement(4319..>4560)
                     /locus_tag="ON498_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3940923.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1578 a          659 c          834 g         1489 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagtaac ccgtggtgct agttaatttt
     4321 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
     4381 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
     4441 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt
     4501 gttttccaag taagctgtgg tctagttttt ctgatttatc catgttttta cgtggcggaa
//
LOCUS       DAJSVD010000162         4535 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSVD010000162 DAJSVD010000000
VERSION     DAJSVD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.165"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(52..522)
                     /locus_tag="ON498_002711"
     CDS             complement(52..522)
                     /locus_tag="ON498_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR3940924.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ON498_002712"
     CDS             complement(681..1139)
                     /locus_tag="ON498_002712"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3940925.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2454)
                     /locus_tag="ON498_002713"
                     /pseudo
     CDS             complement(1310..2454)
                     /locus_tag="ON498_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2467..3384)
                     /locus_tag="ON498_002714"
     CDS             complement(2467..3384)
                     /locus_tag="ON498_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR3940926.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3812..4375
                     /gene="efp"
                     /locus_tag="ON498_002715"
     CDS             3812..4375
                     /gene="efp"
                     /locus_tag="ON498_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3940927.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1406 a          834 c          862 g         1433 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatctttt ggttagaagt cactaaacca gctactacat cgctgtgtcc
     1861 gcctagatat ttggtaccgc tatggacaac gatatcagaa ccgagatcta aaggtctttg
     1921 aaaataagga gtagcaaatg tattgtcagc aataactatc aatccatgtt gatgagccag
     1981 agtggcactt ttttctaaat cagtaatctt tagaagcgga ttgctaggtg tttcaagata
     2041 aagtgctttt gtattgggtt tgatggattg ttctatttta tcaagattac tcgtatctat
     2101 gatcgtgtac tcgagaccat ttttagtaag aactttgtca aatagacgga atgttcctcc
     2161 atagacatca tctcctaata agatatgatc gccagcttga aaaagagaaa aaacggcatg
     2221 aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca ccgccttcca aatcagcaat
     2281 cagttcttcc aatgcaaaac gagtaggatt accagatctt gaatattcat attgtttagg
     2341 ctggccgacg ccatcttgcc gataagtgga tgtttgataa ataggtacac tgacagctcc
     2401 tgttgtggga tcttcactga tgcctccgtg gatcaatttt gtttgaatgt gcatgtttct
     2461 ttcctcctat aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt
     2521 aacgacacga tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc
     2581 accactcgaa cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa
     2641 cccttctaca tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg
     2701 aatgaattct acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac
     2761 agagccctct ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt
     2821 agaagttcca gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc
     2881 tttgatttca tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt
     2941 attttcaaat tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt
     3001 gctgatggca ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc
     3061 tttcatcaat tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa
     3121 tttgtatgtg agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat
     3181 gatcgtcgtt ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc
     3241 caatctatct ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga
     3301 acctttaggc atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc
     3361 ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc
     3421 tgactattaa atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag
     3481 ttacctggta ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt
     3541 tatgcaccat acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc
     3601 ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa
     3661 gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga
     3721 gaccttaaaa gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg
     3781 attatgataa agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg
     3841 tatgactttc gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc
     3901 tggtaaagga aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata
     3961 cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt
     4021 acaatatttg tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca
     4081 atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga
     4141 agtaaaaatc caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt
     4201 attacgtgtt gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa
     4261 gccagcgaca atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga
     4321 attgttggaa atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc
     4381 taacggattg ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat
     4441 ctgcctttca agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt
     4501 ttatttatat tttgaaagat gaatggcaaa agcta
//
LOCUS       DAJSVD010000163         4528 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSVD010000163 DAJSVD010000000
VERSION     DAJSVD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.166"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            107..358
                     /locus_tag="ON498_002716"
                     /pseudo
     CDS             107..358
                     /locus_tag="ON498_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="ON498_002717"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="ON498_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR3940928.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1738
                     /locus_tag="ON498_002718"
                     /pseudo
     CDS             1655..1738
                     /locus_tag="ON498_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1787..1870
                     /locus_tag="ON498_002719"
     CDS             1787..1870
                     /locus_tag="ON498_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR3940929.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="ON498_002720"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="ON498_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3940930.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="ON498_002721"
     CDS             complement(2995..3858)
                     /locus_tag="ON498_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR3940931.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="ON498_002722"
     CDS             complement(3860..4132)
                     /locus_tag="ON498_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3940932.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4358)
                     /locus_tag="ON498_002723"
     CDS             complement(4149..4358)
                     /locus_tag="ON498_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR3940933.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT         1399 a          761 c          837 g         1531 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt ctttttcgtt ttctaattga ataattcgct caagcatttc
     4501 aaaaaatacg ttcttataca tggataaa
//
LOCUS       DAJSVD010000164         4427 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSVD010000164 DAJSVD010000000
VERSION     DAJSVD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.167"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..853
                     /locus_tag="ON498_002724"
     CDS             <1..853
                     /locus_tag="ON498_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR3940934.1"
                     /translation="QRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNL
                     EDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSI
                     ADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLT
                     RTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCD
                     LKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYL
                     PYVNLYD"
     gene            888..1655
                     /locus_tag="ON498_002725"
     CDS             888..1655
                     /locus_tag="ON498_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3940935.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            1846..2244
                     /locus_tag="ON498_002726"
     CDS             1846..2244
                     /locus_tag="ON498_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3940936.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2550..2924)
                     /locus_tag="ON498_002727"
     CDS             complement(2550..2924)
                     /locus_tag="ON498_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940937.1"
                     /translation="MKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLWKYKILFL
                     FWEATLTFSVLFYKSDCYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2946..3323)
                     /locus_tag="ON498_002728"
     CDS             complement(2946..3323)
                     /locus_tag="ON498_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940938.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(3313..3708)
                     /locus_tag="ON498_002729"
     CDS             complement(3313..3708)
                     /locus_tag="ON498_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3940939.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3775..3960)
                     /locus_tag="ON498_002730"
     CDS             complement(3775..3960)
                     /locus_tag="ON498_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR3940940.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4042..4296)
                     /locus_tag="ON498_002731"
     CDS             complement(4042..4296)
                     /locus_tag="ON498_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940941.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         1491 a          678 c          759 g         1499 t
ORIGIN      
        1 tcaaagaact aaacctaacg gtaagactga atacaaagtc atctcaccgc tagaaattac
       61 tgaattcaat gatttttctg atgcagtatc tattaaattt agtagttcaa ttatgccttt
      121 attaacgaac ttagaagatc attttacgca atatacattt gatcaagtag ttaacttgac
      181 aagcaagtat gcgatcagtt tttttcaatt attcactaaa gattttaatc tgtatgaagc
      241 aaatagagat aacttaacat ggagtacaaa atcacttgat agattaaaaa atcctgtttt
      301 ctccattgca gatttacgaa agaaatttag tattgagaaa aaatataaag cttttcgaga
      361 ctttgaaaga cgtgtaatta aagaaccgat tgcccaactg aataaataca ctgattttaa
      421 aattgactat gagcttatca agaagggtca tacttataca aacataaagt tcttgcttac
      481 acgtaccatg tcagttccgg taccttataa gcaagaacaa agagaccctg cttatttaaa
      541 aggcgcagaa gataaaacag agaaaatgaa tcaatcgtac ttgaaagcta tgaatagcaa
      601 atacactaaa ttactaatca aaaacttttt gatcagttct gccgacttat gcgatcttaa
      661 acttatgagc cgacttcaga ataaagttta tccgctttat gatcgtttat ccaaagatag
      721 aggatttgaa tcagttgtca atcacctatc ttacgttgct actcataagg aggattacac
      781 aataaaaaat actgttaaat atctcgaaaa atcaattacg agttatttgc cttatgtgaa
      841 cctttatgat taacaactga aaaaatgcgt gaaggaagga aaaacttttg aaaaaagatg
      901 aactgacaaa gtacaatgag gagttcttag caacaattga catgaaaatg gatttaaagt
      961 cgtatgaact ttatcaattg ctgttcagtt atatctcacc cacaaagcta aagaaaacag
     1021 gcgtagtaga tttacctgtt aaagcgatta ttaattacat ggatgttgat caaaatcttg
     1081 aaacagaact aaaaaatata gttaacaccc ttcagagaca agcagtttat gaaatatcga
     1141 acttagataa agaatataag acttatgcta cagtatctgc tattgatcaa attcattggg
     1201 acttagaaga acaaattata actttcaatt tctcgcaaga agtaattccc ctaattcttt
     1261 caatcaaaaa gaaatatcca tattttgttt ttcaagattt agcaagcttt caaaaaaaat
     1321 atagcattgt gttatacaaa tatttggcga tgtattataa aaaatatctt atcgccaaaa
     1381 ataaaggaga ttttgagaaa gctgaaatat ataagaatcc cctgattgaa atagttaaaa
     1441 ttcgtgagat tacgaaaaca gaaaatgagt acaaaacaat atcgaatttt gatagacgtg
     1501 ttttaaggga atcagttgag gaaattaatg aaaaatcaat gctgaagatt gattttgcta
     1561 aagttagaaa aggcagaaat tacaattttg ctgtccagtt taatatttct agtgagtttg
     1621 atgaaaaacc ttatagagaa aaaaaattag gttaatgcaa aatatatctt actcgtttga
     1681 agatgcttca gacgtatgct aagatatgta gtgccttggg agcaaggtat taatctcctt
     1741 tagaggttaa ttagcttgtg atcgcctatc aatttttttg gtaggcgatt ctttttttga
     1801 aactaaaaag caaaaattat tttttaatta gagaggtgca atgcgatgaa atactttttt
     1861 tgtgcagtat tcgaatggaa aggaaatgaa tatgcagtta aattcccaga ttttccagac
     1921 ttatatacgc atggtgaaac tttagaagat gcttttgaga tggcgcaaga agtattggaa
     1981 ggatacttgg tgcttttaga gttgcaagaa aaagaaattc catcgcctac gcctgttcaa
     2041 gagttaaatg caagaatcaa gcgatctgag aatgttaatt tatttgtcgt aaaagctaat
     2101 acgaaagttg ctaaagctcg aaacaagcaa aattacgatc gaaaatcagt gactgtggaa
     2161 cactatttaa atgttttagc ggctgaagag aatattaatc tgtcgaaagc tttgaatgat
     2221 ttgttgagag aaaagttgat ttgatgtctc tcagatgatc atattaagca aaaacagctt
     2281 gttttaggac gtatggattt aagaaagaca aattatcaaa gaatatatta aaagctctca
     2341 gaagcgttct gagagctttt aaattgctat gtattatact gcatctgctc aaattgattt
     2401 tcttttcatg cttaccatgt atctagtttt ccatcaagta atttttagat atgtattgag
     2461 gaataaatag caaatttgct tgatataagc tcgaattagg tgctttgtaa tgtgtttgga
     2521 taaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt tttatcggta
     2581 ggcttgttta acgtgatctt gaaagatacc cttttgatgt ttctctcgat ccctgcatcg
     2641 ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat caaaatggtg
     2701 tagataagca aaaaactccc taaaactaag tgttgattat aacaatctga cttataaaaa
     2761 agaactgaga atgttagtgt tgcttcccaa aaaagaaaca aaattttgta tttccaaaga
     2821 tagtggatca agcgctgtgc tttactcatc tcaaataatt tataatatcc tgctaattga
     2881 tcagcaagcg tatcgttaat gggttctgtt tgtgtttgtt tcatctttct cttctcctgt
     2941 ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc ggtttgaaat
     3001 tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga ttcacttaat
     3061 gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt atagcagaat
     3121 tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa gttattatcc
     3181 tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc agttgtatct
     3241 tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc tttcataggt
     3301 tcggtctcct ttttactttt caagcaactc ttcttctagt gctttttgaa gtagttgaga
     3361 gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac taacattttt
     3421 tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt ggatgattac
     3481 taccacttga ttggctttca aagttaattc ttccactgct gatggtttag gaaattctgc
     3541 atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat atgcttcttc
     3601 aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt tataagcttc
     3661 tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt cctcgctcct
     3721 tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag gctgttaaag
     3781 ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat tgtggatcgg
     3841 aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc cagtttgtcc
     3901 agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg tcttaggcat
     3961 agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat caatagaaaa
     4021 agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag caaattgaat
     4081 gttattagcg ttgcaaatat tcataatttc ccaagtatca ttagaagtat aggtatctaa
     4141 gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt tctccaagtc
     4201 ttttgatgaa atgatcaaga ctttttcaat ttcctcccct ttttcatttg tgaaaacgta
     4261 ttccaaagtg atttgttctt catcggtata attcatattg tgttctcctt tcttgtatta
     4321 agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata agacgtgacc
     4381 gagcgttacg cctttgaaat gctcttctaa atacatctaa acgcatt
//
LOCUS       DAJSVD010000165         4413 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSVD010000165 DAJSVD010000000
VERSION     DAJSVD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.168"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            3..2093
                     /locus_tag="ON498_002732"
     CDS             3..2093
                     /locus_tag="ON498_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940942.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            2110..2259
                     /locus_tag="ON498_002733"
     CDS             2110..2259
                     /locus_tag="ON498_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940943.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            2256..2702
                     /locus_tag="ON498_002734"
     CDS             2256..2702
                     /locus_tag="ON498_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940944.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2704..2841
                     /locus_tag="ON498_002735"
     CDS             2704..2841
                     /locus_tag="ON498_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3940945.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2879..3172
                     /locus_tag="ON498_002736"
     CDS             2879..3172
                     /locus_tag="ON498_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940946.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            3169..3393
                     /locus_tag="ON498_002737"
     CDS             3169..3393
                     /locus_tag="ON498_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3940947.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            3390..>4413
                     /locus_tag="ON498_002738"
     CDS             3390..>4413
                     /locus_tag="ON498_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3940948.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         1562 a          729 c          915 g         1207 t
ORIGIN      
        1 aaatggctag cagtttatat aatttggctt tagatttcag caaagaatta aactacacca
       61 aagctattat ggctcgtcaa ggtgataaag ggattacggt gacggttaaa ccgtttctaa
      121 atggcttgca gatggatacg agtggcggaa catttacttt aaaaggaaca acaccatcta
      181 accgttacgt agataatgtt gcaactagtg taactagtga agaagtcacg ttttctcttg
      241 atggcacatt tatgagtgaa gcaggatatt ataaacactg ctacgtagaa tatagaaaag
      301 acaatcaaat tttaacaacg caagatatca tttttttctc actaggagtg tctgacattt
      361 cgcaaggcca agccgatgaa tatgtttcgc aattagaaga gttgattcga aagtacaacg
      421 aaacttttga tgcttttatg gctgaaatca aaggtagagt ggatagctta aatcaacaga
      481 ttactgattt aactggtcaa gctaaaacgc tacaagacaa gttagatgct ctgaaagaag
      541 aaatttctaa gttaggtaac ttacaagtga tgtacagtaa cagcatcgac ttcgggggat
      601 atgattatag cggtaagccg aatttaatgt ccaaactaaa atcgagcgat tttaacgttg
      661 gttaccacgg gtcactaact ttggataacg aaaagttaca ttttacttct gatggtacag
      721 gaagcattat tatgtttacg cgtattaata cacctcagct tactagtggg aaaacctata
      781 ctctgagtgc gaaagttcga tttgatgaag gaactacagg agctattgat aaattacgtt
      841 tggtgtatcg tacatcacca ggagaaaaga tattattgga agcaaatagt acaaatatta
      901 caacagatga tgtagggaaa gaaataacaa tcaaaggtac agctaacgtt aattatcaaa
      961 tcacaaattt agatcgattt tatatgagta ttagctttgt tgacagggat aaaataaatg
     1021 gcggatttaa gttgtacgac atcaaaatcg aagaaggctc aacagccaca ccgtaccagc
     1081 ctaatctact tgatgcaccg tattatttga gtaaggtggc tttgggtgag aatctaatta
     1141 aaccagaatc acaacaacca gttactaata gtaactatct tattaacacc tataacatta
     1201 aaccaatggt aaaaggtaag aagtatacca tcacacttga aggaactaag ccagcaacac
     1261 aggtttttag accatttttc acacgagcaa caggagatgc atgggaggtt ggtgacttac
     1321 aaccagtaga aggcttaact aatgtgtggt ctaaaacatt tacagcagca gatgactcac
     1381 accctactac cccacaagtg cagatttatc aagtaccaag cacaagtgta ggacaatgta
     1441 caattaagtg gttaaaacta gaggaaggca atactcgaac cccgaatatt agtgagtata
     1501 aatatcgtgg tactggtatg cgtgattcaa acaatccaaa agattatgtt tgggatctag
     1561 caccagaata tgtcgaagat aatttggcca cagatattaa aatttctgaa attactggta
     1621 aagcaaacaa ttataccgat gggaaagtat cggagattaa ttcgcagttg actgcttcaa
     1681 ttaatgaagt agacaccaca gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg
     1741 ctatagatga attagacaat aagatcgatg aacgcattaa tgatacagct actaccacat
     1801 taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga aaaacagttt
     1861 ctatatattt tgtggcttta aacggaaaac gcagtggtgg aaatgattca acgatactaa
     1921 caattccaga aggctatcgg ccaccaatta gttttgagca actggttggc tcgatagacc
     1981 gttctacttt gaacagtgct cagttatcta ttggtgcaga tggagccatt aaatggcgaa
     2041 gaaactcaag ttatggatcg gattatacct ttgcaattac ttacacgatt tagaaaagcg
     2101 tgaatcgata tgaaggcagc atatagacca attgaacctt acggattcga gcaaatcatt
     2161 gtgaatgatg aagaacattt accggaagaa tgcacagaag tcgaaccacc gattccaaat
     2221 tggaaaccga aattcaatta ctgggaggga aataaatgaa aaacatttgg aaatatggac
     2281 gtactggcgg agagtacgca ggaaaagtat tggacgacat gcttgtatcc gttccttaca
     2341 cagatcagcc accgcttgaa ggaattcgtg ctgatggcga accgttaaca atcgctgatc
     2401 aaatgtttga tcctaaattg aatcaatgga ttattttagc gaacgcacta gatcacaacg
     2461 atttaaacaa tctcaaagca atgtatgagt cgttagaaaa tgagaacggc gatttaaaac
     2521 agatcaatgc caaactcatg ctaagcgatg tagcaattaa acaggaaaat actgcattga
     2581 aagaaaaagc ggatagttta gcacaaatca attcaaaaat gatgcttgct tcgttacaaa
     2641 atagcaaaga catttcagaa attaaagagc aactaaatcc agcttcaaag ggaggtgagt
     2701 agtatgttta gttttagcga tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa
     2761 caagtaatgg ttttcgttcc gttgtgcatt actgaagaaa aagcagataa aatcattagc
     2821 aaagaagaga gcgcatctta attgatgtgc tttttatttt gattcaagga gttgtcacat
     2881 gattaattta ggggaatggg gagcgatagc aggatcaata accgctatcg tttctttgat
     2941 tttattagta ataaaaccaa ttactgcatc tttctcgaag attactgaga ctctttcaaa
     3001 agtaagtcac aatttagatt tgctgactaa agatttagaa tcgagcaaat cagatcgatt
     3061 gatgattcat gaagaactaa agaaacacga tgaaagatta gatacacatg cagaaaaatt
     3121 ggtagaacac acgcaacaaa ttaaaacttt atttagggag agaagaaaat gaataataaa
     3181 acgttcgaag tactaaaatg gttcgcactg gtaattattc ccgcactagc tactttcgtg
     3241 gggttagttg gtaaagcgct caattggcag tacacagata tctgtgttgt catcattact
     3301 ggttttggcg cgtttttagg gagtgtgttg ggtgtatcaa atcgaaccta caaaatgttc
     3361 tcggctgaaa gcgaagaagg aggaaacaaa tgaaaaagaa aattactatt actgcgatga
     3421 gcctgttaac ggctcttttt ttattaccca ttaatacgtt tgcttatact attaatgacg
     3481 agtataattt agcgccgaat caaggagact ccagattagc aattcctaac aaaattattt
     3541 tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg aaaaataatt
     3601 ataacggaag caattcttat actacagatg ttattggtga cggtgggatt gtttaccgtg
     3661 tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat gcgcctgtac
     3721 agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat cgagcttata
     3781 ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt gatcaaggaa
     3841 cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat gtttggggga
     3901 accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa aagcttgctt
     3961 atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac aagcctgtca
     4021 ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca aatcacgccc
     4081 acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga tggcacattg
     4141 ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
     4201 gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat acttctggaa
     4261 atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa gtctatgtaa
     4321 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
     4381 acaaacgttg gtatttaaat attcctaaac gat
//
LOCUS       DAJSVD010000166         4372 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSVD010000166 DAJSVD010000000
VERSION     DAJSVD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.169"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(83..1051)
                     /locus_tag="ON498_002739"
     CDS             complement(83..1051)
                     /locus_tag="ON498_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3940949.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="ON498_002740"
     CDS             complement(1496..2902)
                     /locus_tag="ON498_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR3940950.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="ON498_002741"
     CDS             complement(3124..3486)
                     /locus_tag="ON498_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940951.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3828)
                     /locus_tag="ON498_002742"
     CDS             complement(3490..3828)
                     /locus_tag="ON498_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940952.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3807..4226)
                     /locus_tag="ON498_002743"
     CDS             complement(3807..4226)
                     /locus_tag="ON498_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940953.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4219..4359)
                     /locus_tag="ON498_002744"
     CDS             complement(4219..4359)
                     /locus_tag="ON498_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940954.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1241 a          979 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DAJSVD010000167         4365 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSVD010000167 DAJSVD010000000
VERSION     DAJSVD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.170"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            16..246
                     /locus_tag="ON498_002745"
     CDS             16..246
                     /locus_tag="ON498_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR3940955.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            343..1644
                     /gene="murA"
                     /locus_tag="ON498_002746"
     CDS             343..1644
                     /gene="murA"
                     /locus_tag="ON498_002746"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3940956.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1657..1833
                     /locus_tag="ON498_002747"
     CDS             1657..1833
                     /locus_tag="ON498_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3940957.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1968..2264
                     /gene="yidD"
                     /locus_tag="ON498_002748"
     CDS             1968..2264
                     /gene="yidD"
                     /locus_tag="ON498_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3940958.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2328..2711
                     /locus_tag="ON498_002749"
     CDS             2328..2711
                     /locus_tag="ON498_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3940959.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            2880..3764
                     /locus_tag="ON498_002750"
     CDS             2880..3764
                     /locus_tag="ON498_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3940960.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3878..4114
                     /locus_tag="ON498_002751"
     CDS             3878..4114
                     /locus_tag="ON498_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR3940961.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4202..4282
                     /locus_tag="ON498_002752"
     tRNA            4202..4282
                     /locus_tag="ON498_002752"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4236..4238,aa:Tyr,seq:gta)
     gene            4289..4360
                     /locus_tag="ON498_002753"
     tRNA            4289..4360
                     /locus_tag="ON498_002753"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4321..4323,aa:Gln,seq:ttg)
BASE COUNT         1478 a          686 c          977 g         1224 t
ORIGIN      
        1 agaaaggacg tcattatgca agtatacgga attgatgcgg tgcttcgaat cgtgagtcat
       61 ttggcgttta tctacttagc cttctggtct ttacgttcgt tacgcattga aacttttttc
      121 agaccaatgc ataatacgca aattagaatg gcaatcgtac ttttttcaat ttttttaggg
      181 tacactgcaa gtaatttttt cttagaagtt atcgctttat gccgaaatct tttcgttagc
      241 ctgtaaattt taaaaaaagg tactatttta aaagcaagga tgttataata gaaaagattt
      301 tgagtaagtg tatgctcatt ggaatttttt ggagggaaca acatggaaga gatcatcgta
      361 agaggtggca atcaattaaa tggaacagta agaattgaag gagctaaaaa tgctgttttg
      421 cctattttag ctgctagttt gttagcagaa gaaggtatta cgacattaga caacgttccg
      481 attctttctg atgtcttcac tatgaatcaa gtgattcgtc acttaaacgt agatgtagat
      541 tttgatgaac aaaagaatca agtaacaatc gacgcttcaa gacaattaga aatcgaagcg
      601 ccttatgagt atgttagtca aatgcgtgct tcaatcgttg tgatgggtcc gctacttgcc
      661 agaaatggac atgcaaaagt agctatgcct ggtggatgtg cgattggtaa acgaccaatc
      721 gatttgcatt taaaaggatt tcaagcttta ggagcaaaaa ttattcaaaa aaacggctat
      781 atcgaagcaa ttgcggatga attgatcggt aacacgattt atttagattt ccctagtgtg
      841 ggtgctactc aaaatatcat gatggctgcc gtaaaagcaa aaggaacaac catcattgaa
      901 aacgtagctc gcgaaccaga aatcgttgat ttagcaaaca ttttgaataa aatgggcgct
      961 caagtctatg gagctggtac tgaaacaatg cgtattgaag gcgtggatca tttacatgca
     1021 gtcaaccact caatcgtaca agatcgtatc gaagcaggta cctttatggt tgctgctgca
     1081 atgacacaag gcaatgtgct gattgcagat gctatctctg aacataactg cccattgatt
     1141 tctaaattga tcgaaatggg tgcagaaatc attgaagaag aaggcggagt ccgtgtcatc
     1201 ggaccaaaac atatcttacc tacagatgta aaaacgatgc ctcatcctgg tttccctaca
     1261 gatatgcaag ctcaaatgac agctatccaa ttagttgcag aaggtacaag tgtggtgact
     1321 gaaacagtgt ttgaaaatcg tttccaacat ttagaagaga tgcgccgtat gaacgctcat
     1381 gtcaaaatcg acggaaatgt agcaatcatg gatggaaacc atgaattgca aggtgcagaa
     1441 gtatatgcaa cagacttgcg ggcagcagca gcattagttt tagctggctt gaaagcaaat
     1501 ggaatcacac gtgtgcgtaa cttgaattat ctagaccgtg gatattataa tttccatatc
     1561 aaactacagc aattaggtgc agacgtcgaa cgtgtagaca tggatcaaac ttctgctgaa
     1621 aaaacagcac aaacgattgc ttaaggaagt ggctgaatga gtaaccaacg atatatctta
     1681 ttaactttga taaagatttt agtggtgatc ttattgctta tccttctttt tgtggccgga
     1741 acgatgatcg gatatggtgt catcggtggc ggcaatccat ttaaagtatt ccagccaagt
     1801 ttgtggattc atattcgaga cttttttcat tgataaataa gaatcacagt ggttctagtc
     1861 gatatgaagt gagccatgaa gcagaacatg ataaaagaaa acagaaggga acgttgacgt
     1921 tagtcgcgat cccttttttt agtaaaagaa aagaaggaat ctaattgatg aagaagatat
     1981 tgatttattt agttagagga tatcagcgct ttatttcgcc tgcatttcca cctagctgcc
     2041 gttattaccc aacctgttcc aattatatga taaaagcaat tcaaacccat ggtgcgctaa
     2101 aagggacggt aatggggact gcacggatct tacgctgtca tccttttgtc aaaggcggta
     2161 tcgattatgt accattgaca ttccgattaa cgcgaaataa gaaagataaa aaagaaccag
     2221 attataaaaa ggttgtgaaa aatacgggta gcactgagcg ataaagaaaa agaggaaatt
     2281 tattgtcaaa gtaacaactc cgcaactact tatatggggg acaaaaaatg atcgaagaac
     2341 taaacaagaa agatttacca tggtccttgt tattagatgc agatcctgat aaagaaaaag
     2401 ttcaagtata tgtttccaga gggagtggct ggatatggaa agaaaaagaa aaaacaatag
     2461 gtgtactgat ctatgtggca agggaaaaag aatacgaaat cgtcaatgta gcagtaaccc
     2521 caacgtatca aggaaagggg attggcggaa aattacttga gacagctttt cagaagcttt
     2581 cacagctagc ttcaaaccaa actcggatca ttatacgtac tggcactact agtagcgctg
     2641 cgttacacct ctatcaaaaa atgggctatg tggagattgg tcgagtcaaa gactatttta
     2701 tccatcatta atctgagccc atttttgaaa atggagatca attaagggat caagtgattt
     2761 tagcaattca cttacctaaa aatagcttta actaataaac tttcaattcg agaaaatgct
     2821 ttcaaatgct aaatagatac attacaatag ctttagcaaa caaaaaggag aatattttta
     2881 tgggaatatt agtttttgat tttggtggat cggctgtcaa atatggttgt tgggatggca
     2941 aagagattaa aggtaaggga caatttgcaa cgcctgaatc ttgggaagaa atgaaaacgc
     3001 aattatttca agtttataaa aaaatactcg tttcttttga agtcgaagga attggaatca
     3061 gctctcctgg agtagtgaat aatgagaaac aggtgattga gggaatcagt gcgattcctt
     3121 atattcatgg atttaatatt ttccgagatt tggaaactct gtttcaactc ccagtaacga
     3181 ttgaaaatga cgccaactgt gccggaatgg ctgaatttta tgaaggtgct gcccgtgatt
     3241 atcaagatgt tgcttttgtt gtggcaggaa ctgggattgg cggggcgttg ttcacgaaag
     3301 gacaaataaa taaaggggca catctttacg gtggagaatt tgggttgatg ttcttagatg
     3361 gcgataagac attcagtaaa cttggtacag cggtacagat ggcttggcgt tattgtgaac
     3421 gcaaaggtct cgataaaaat gcttataccg gaaaagatgt ctttgaatta gcagaaactg
     3481 gagatgcaat agcaaaagaa gaagttgaga gtttttatac gtatcttaca aagggattat
     3541 tcagtattca attttcgttt gatccagaag tgatcgtatt aggcggcggg gtttctgcaa
     3601 aagaagggct cattgatgag atcaaaagcc gaatgaagaa gctgacagaa caattcgatc
     3661 tgcatgattt cgaaccgcag attttattat gtgagtacag aaacgatgct aatcttgtag
     3721 gtgcagcagc aaactatctc gctgtacgtc aaggagagaa ttaagagctt tagagaaggt
     3781 gcagcttgca taaaagaaga gaatgaaatg aataggagat ttttacctaa ggaatgtttt
     3841 atttgctgca gtatgataaa atatctgaga ggtgaaaatg atgtcaaaaa aaaataaaga
     3901 tattgaagtg cgtatagaag aagtgaaaaa agatatcaaa ggtaagaatt atgaagtcac
     3961 tcagttattt atagggaaaa aaatgattgg tgagatctta gcctatgggc caaaagaata
     4021 tgaaattttc tttggtgaag aagattttgg aaaagaaaaa agtcttgaaa atgcgataga
     4081 aacagtgatt cgacactgga atctgcacga ataaaaaaat atcaaaaaaa ctttcaataa
     4141 agggttgcaa aaatctggaa aattaggtat tataactaag tcaggttgat acaaaccttt
     4201 cggaggagta gcgaagtggc taaacgcgac ggactgtaaa tccgttcctt agggttcagt
     4261 ggttcgaatc cactctcctc catttcattg gggtatagcc aagcggtaag gcaagggact
     4321 ttgactccct catgcgttgg ttcgaatcca gctaccccag tatac
//
LOCUS       DAJSVD010000168         4360 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSVD010000168 DAJSVD010000000
VERSION     DAJSVD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.171"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1743
                     /locus_tag="ON498_002754"
     CDS             <1..1743
                     /locus_tag="ON498_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3940962.1"
                     /translation="AKETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSL
                     GRVQTPTLYMIYQLQEKIRNFKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFED
                     YLKEKGVQLGKQPGMIRQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQG
                     LYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKV
                     QEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQL
                     QLKAIGKVPIDLGWKKLFGKENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQP
                     YTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKL
                     VVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLE
                     VVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDF
                     KLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEK
                     KKTNFKKRTRRTTK"
     gene            1759..1908
                     /locus_tag="ON498_002755"
     CDS             1759..1908
                     /locus_tag="ON498_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940963.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1982..2575
                     /locus_tag="ON498_002756"
     CDS             1982..2575
                     /locus_tag="ON498_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3940964.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            2591..3490
                     /locus_tag="ON498_002757"
     CDS             2591..3490
                     /locus_tag="ON498_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3940965.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            3493..3978
                     /gene="ssb"
                     /locus_tag="ON498_002758"
     CDS             3493..3978
                     /gene="ssb"
                     /locus_tag="ON498_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3940966.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         1605 a          765 c          899 g         1091 t
ORIGIN      
        1 gcaaaagaga cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta
       61 attgggatga acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga
      121 agcttttcct taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa
      181 aagattagaa atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa
      241 aatggcgcat ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt
      301 gaagattatt taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa
      361 gttgaaaccg agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag
      421 tctaaaatga atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc
      481 ttgtatgaag ggaaatatct aagctatcca cgaacagaca ccccttatat tacggaaggg
      541 gagtatgcgt atttgttgga tcatttagat gaatacaagc attttttaaa ggccgaatca
      601 atcccaaccc ctattcatac gccgaatagt cggtatgtga ataacaagaa agtccaggaa
      661 cattacgcga tcattcctac aaaaacggtt atgacagcag cggcttttga acaactaagt
      721 ccactgcagc aggccattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa
      781 aaatatacct atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca
      841 atcggaaaag taccgataga tctaggttgg aagaagttat ttggcaaaga gaacgacgga
      901 aaagagaagg aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta
      961 gatctacaag tccttgaaaa agaaaccaag ccaccacagc cttataccga aggtacgttg
     1021 atcacggcga tgaaaacagc tgggaaaacc gttgatagtg aagaagcgca atccatattg
     1081 aaagaagtgg aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa
     1141 caaaaggaat atatcaaggt agagaaaaat aagctcgtgg tgacgaataa agggattttg
     1201 ctttgccaag ccgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg
     1261 gaaagctact tgttaaagat cggtgaacga aaaggaacac aaaccacttt tctagccaat
     1321 atccaaaaat ttgtctctca cttattggaa gtcgtaccag ggcaaatcca atccactgat
     1381 tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat
     1441 ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagca
     1501 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
     1561 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
     1621 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
     1681 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
     1741 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
     1801 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
     1861 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
     1921 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
     1981 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
     2041 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
     2101 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
     2161 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
     2221 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
     2281 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
     2341 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
     2401 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
     2461 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     2521 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     2581 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     2641 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     2701 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     2761 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     2821 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     2881 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     2941 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     3001 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     3061 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     3121 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     3181 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     3241 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     3301 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     3361 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     3421 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     3481 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     3541 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     3601 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     3661 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     3721 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     3781 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     3841 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     3901 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     3961 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     4021 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     4081 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     4141 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     4201 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     4261 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     4321 ataattttag cgaggtgatt gtgatgaaca aatatgttgg
//
LOCUS       DAJSVD010000169         4317 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSVD010000169 DAJSVD010000000
VERSION     DAJSVD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.173"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(30..926)
                     /locus_tag="ON498_002759"
     CDS             complement(30..926)
                     /locus_tag="ON498_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3940967.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(919..1599)
                     /locus_tag="ON498_002760"
     CDS             complement(919..1599)
                     /locus_tag="ON498_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3940968.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1600..2382)
                     /locus_tag="ON498_002761"
     CDS             complement(1600..2382)
                     /locus_tag="ON498_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3940969.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2375..3292)
                     /locus_tag="ON498_002762"
     CDS             complement(2375..3292)
                     /locus_tag="ON498_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3940970.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3456..3779)
                     /locus_tag="ON498_002763"
     CDS             complement(3456..3779)
                     /locus_tag="ON498_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940971.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1264 a          945 c          756 g         1352 t
ORIGIN      
        1 ttctactagt tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag
       61 aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt
      121 gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa
      181 aaacgctgga agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact
      241 gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa
      301 cgcaataaat tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat
      361 acagttgtat ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt
      421 tcataaaaag agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc
      481 atttcttttt gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt
      541 aatcttgcaa cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct
      601 acctgttcca gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga
      661 gaaaggccga cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt
      721 tgttcatttt catccaacca tgtattcagt gcatctacta ggtccattag attcttttct
      781 cgaaattcga ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc
      841 ttagttaatc tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca
      901 acgagaagca acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata
      961 cacagcatgt gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt
     1021 ctcactatca aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac
     1081 ttttttcgga tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac
     1141 agccacacca ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata
     1201 gatgcgttgc gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat
     1261 tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc
     1321 caacaaggaa actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt
     1381 cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc
     1441 tagaaggatc aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata
     1501 agcatcaaga agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc
     1561 atcgtcttcg ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg
     1621 gaaaataaac attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat
     1681 tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc
     1741 cgtaaaccac tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa
     1801 ggccgttccc atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat
     1861 gaatgtcaaa gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa
     1921 tacttttaga acataatttc catagttttt gattccctca acttcaccaa atccgaaagc
     1981 aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa
     2041 aagcgtaacg agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt
     2101 attgcgaatc gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa
     2161 agccagaaga atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat
     2221 gactgtatcc gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt
     2281 ccaagaataa aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct
     2341 cttccatact tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta
     2401 attgtaagaa ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt
     2461 ttgcttctac caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga
     2521 taacacggct agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg
     2581 cttgttccgt cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt
     2641 ctttcgataa ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct
     2701 gtgacaattc atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca
     2761 aacgttggat gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt
     2821 catccaaaat caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc
     2881 gcatacctaa agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt
     2941 tcagcaggtc ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt
     3001 tatctcttgc agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc
     3061 gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga
     3121 tcaaacccat aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc
     3181 catagatcat tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt
     3241 attttttggt tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc
     3301 ttctttctta ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt
     3361 ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc
     3421 tttcctatta tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg
     3481 accaaagtcg tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc
     3541 ttgagtaatt ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa
     3601 attagactgt ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt
     3661 gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct
     3721 tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt
     3781 aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta
     3841 ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt
     3901 ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg
     3961 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa
     4021 taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat
     4081 agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg
     4141 tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta
     4201 cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa
     4261 gctttacggg ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaa
//
LOCUS       DAJSVD010000170         4301 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSVD010000170 DAJSVD010000000
VERSION     DAJSVD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.174"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..583)
                     /locus_tag="ON498_002764"
     CDS             complement(<1..583)
                     /locus_tag="ON498_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940972.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLT"
     gene            complement(737..1063)
                     /locus_tag="ON498_002765"
     CDS             complement(737..1063)
                     /locus_tag="ON498_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940973.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1100..1465)
                     /locus_tag="ON498_002766"
     CDS             complement(1100..1465)
                     /locus_tag="ON498_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR3940974.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1502..1717)
                     /locus_tag="ON498_002767"
     CDS             complement(1502..1717)
                     /locus_tag="ON498_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940975.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            1846..2181
                     /locus_tag="ON498_002768"
     CDS             1846..2181
                     /locus_tag="ON498_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940976.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2257..4254)
                     /locus_tag="ON498_002769"
     CDS             complement(2257..4254)
                     /locus_tag="ON498_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940977.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1329 a          846 c          560 g         1566 t
ORIGIN      
        1 ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag
       61 tatttatcgt gattccttta tcttcattta caagtttcca agtaggtggt atagctgtgt
      121 atgtttctac aaatgaatta agtttttcat taagtgactt ctcttttata tttccatcta
      181 aaaactctgt atatatggct tgagctatac taaaacaaag ctggccttgt tctaataatt
      241 ttacatcatt agttagtaaa gctgtgttca cgttatcaat cgcttcaaga agagtatctt
      301 tggtgtccct aacagcttta ggtgtgcctt ctggaatttc cctacttgat acctcgtcca
      361 ttttatcttc cataaacccc ttaaaatcat atctttctcc caaatccata gaagtttttg
      421 agaaagcttt catttctgag tctatttctt caataatatt taacatgtct gatacatatt
      481 gtttaggctg attcgttttt aatctatatg tggtaaatat ctcaattcct ccaattatca
      541 ataaaattgc caaacaaatc cataataatt ttttcttttt catttttcct cctatacact
      601 gctgttataa cagtgactaa tgtaaagatt ttcccagcaa tcatcagact attatttaca
      661 gggaatcaaa aaaactcctt agtaagctta cacctttagg agttcctgtt tgaacggtat
      721 gcataataca tttaaatcaa tttaacatta acacctcagt atatggtatc tctacatata
      781 cacggtcttt ttttggatag tactcgacat ttatattgat agcacttcca ttgtctaacg
      841 tagctttagc ccggtacgtg ttatcaccat tatcatgagt gatcgtcaca tcatcacatg
      901 tccgatttaa gttatactgt tcttgtaaaa tctgagttac caatggtaca gatgacttct
      961 ctagtccaga tgaacccgtc attcttgaaa aaattatacc tataatagcc aatacaacga
     1021 tacctataat gacaaacttc ttattcatat ttactttatt caacttttta tctcctatct
     1081 ccaaaatgat tttctctcat cagtaactca ttgacatccc caatagtaca ccaaagatgg
     1141 ctccagctac ccctgctatc atcaaaatcg tacccatttg ttcatctttc tctcgatata
     1201 agtaacccat aataacagca acagcaccaa ataccacagg aataaatgct aatgatacta
     1261 ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt
     1321 gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg
     1381 tctcatctgt ttcttgtaat gctccgcaaa actggcaata cttactttca tttgttattt
     1441 ctttaccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta
     1501 gctaactgtt tactttatta aatacatcag aaaacagctc atttattggt acacttaata
     1561 tctcagaaag cattttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac
     1621 ttacacattg ctgtgtaaca ttcatttttt tggctaactc agtttgggaa agtccttttt
     1681 cttttctgta tcggctgaga ttacttactt ttttcatgat tctccccttc tgttttatta
     1741 tatacaacaa tatgatgtat ttcaactcat aaaatatata aatacttcaa atgtcattac
     1801 aaatacaaca acttgttgta ctctactgag aaggggggtg tattaatgaa acagaagcaa
     1861 tatgcaaagg tcattagaga aaagcgaaaa gcactcgggt ggacacaaaa agaattagct
     1921 aaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagaacct
     1981 aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta
     2041 gacaatgttg acatagatta tgaggaagag tttttggctc tctatcgctc tcttagtact
     2101 gaagatgcaa ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     2161 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     2221 gagatgaaat aagattaaga gctatggata acctgtctag tttaatataa ttgggtctct
     2281 tctattcctt actctccgtc catcatctct tgttacactt gtaaattgat aaataggaac
     2341 taattcaact cctgataata tcaattcttt tattacttca tctatccctg cccctttcgc
     2401 tagatatact aaaaaactac tgcttgcttt cacattgtat agtttcttaa tatccttaga
     2461 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgttttcta ttaaagcatt
     2521 tttcacgctg ttatccctga catttcgtag gcgttcacaa acagtgttaa taagttcttt
     2581 ttctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     2641 taaaatcaat tgtttttcct tgctaagttc tgatcgggaa ttttgaggaa atactctttt
     2701 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     2761 atgaaatttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     2821 attaaaaacg tctaatcgat agacattgct tctcttatgt ttaaagtttc ctaacgcttt
     2881 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     2941 tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc
     3001 ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca
     3061 agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac
     3121 acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc
     3181 aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa
     3241 gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag
     3301 catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat
     3361 ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag
     3421 acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc
     3481 ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt
     3541 catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc
     3601 atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg
     3661 aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata
     3721 tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt
     3781 tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag
     3841 aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa
     3901 cgcagaatta tgaactaaat tgatagataa aaccttttct atgcttaagt atctactatc
     3961 atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag
     4021 aataattact ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct
     4081 tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct
     4141 atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc
     4201 taataatttt tctttatcta cataaattgg aatattaatc attgatgttt ccatttgtgt
     4261 ttcctcctta taaaattctg ttaaagacta aaacatacag a
//
LOCUS       DAJSVD010000171         4288 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSVD010000171 DAJSVD010000000
VERSION     DAJSVD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.175"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(313..843)
                     /locus_tag="ON498_002770"
     CDS             complement(313..843)
                     /locus_tag="ON498_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3940978.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1025..1681)
                     /locus_tag="ON498_002771"
     CDS             complement(1025..1681)
                     /locus_tag="ON498_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3940979.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1808..2281)
                     /gene="dfrF"
                     /locus_tag="ON498_002772"
     CDS             complement(1808..2281)
                     /gene="dfrF"
                     /locus_tag="ON498_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCR3940980.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2700..2918)
                     /locus_tag="ON498_002773"
                     /pseudo
     CDS             complement(2700..2918)
                     /locus_tag="ON498_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2949..4163)
                     /locus_tag="ON498_002774"
     CDS             complement(2949..4163)
                     /locus_tag="ON498_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3940981.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1311 a          918 c          635 g         1424 t
ORIGIN      
        1 attttttgaa atcaactgtc tttctcatat ttactattca attttcatag tgatataggt
       61 acaatttata tcactactgt aaaaatgggg ttttgcctta taaatacagc gttttccgct
      121 tgttagtgta tagtgtttct tgttttttgt gccccccttg ttagataacg ggggctttga
      181 acggctcgca agctcgccga accgccgaag cgaaccgccc ccttgcaagc atggcggaaa
      241 aatcttaagg attttcccgc caacgtggtc ggatcgctcc gtcggtttaa cagcttttct
      301 gtttctctct agttatctac gcatataaat caaatcggtt attccgttat aagtctgtgt
      361 tttacaaagc cttaacttta tttccctgtc tatgttgccc aaaagacgaa ccccatttcc
      421 taacagagta ggaataacag aaatgtaata tttatcaatc atatttttgc ttaccaactg
      481 ctgaacaaga tttgctccgc cacaaatcca aatatccttt cctgcttctt gttttagccg
      541 ttccaataat aaaacaggat tttcacttgt aaaatgaatt tgttgggaag aattgcactc
      601 tttatgtgtt aatacataag ttgtaaattt attatatacc cattcttgcg gagaaagttc
      661 agtaacaacc tgatgagaag tattccaccc cattaagaca gtatctatat ctttggtaaa
      721 ttctgtataa gtgtcgatgt tttcattgtc atttccatga ccatttagcc aagctactcc
      781 accattaccg tccgcaatat atccgtcaag gctcatggca ataaataaaa ctacttttct
      841 cattttttct cttttataaa tacaaacagg aatttatttc tcttttttac tcataaggga
      901 acaactgatt gctacaaata aacccattaa aatccatgga aatgctgctc ttataaaatc
      961 actaaccata ctttattcct ccttaaattc caatttcctt agttctacaa actgaaattt
     1021 ttgcctatga atgtatcttg ttctttatac gcaaacaggc ttctgtgtgc ataatccaat
     1081 gtcttgaaaa cggcatttga attagtccac aaatatcttt attacaccaa taatcaatca
     1141 gccaaattgc tttttcattg tcgtttacta cattcaacga ttctaagtat ccttttcttt
     1201 ctttcggtat ttttcttttc aattcatcat aagataaccg ttcaagcatt ttctcagtgc
     1261 tttcccatac ttcgaaaata tataaatata attcttccag attaagctgt tttgaaaagt
     1321 ctgcaatctg ctgtttcatg agttcatttc ctgttgtaat gataggtgaa ttgatacgct
     1381 cttgataatt gcctgcaaaa aatacttgtt catcttcatt tatcactgta tgcacaacta
     1441 tatcttcaat gcggaaaata tgccaaatag aataagcaat cgtcttacta tgataaccat
     1501 cagcgtttat gaaggggatt gagttaaaat cttctctgca taattcttct ttgaaagata
     1561 ctaaggtttg tattaactga cttcgtaaag taagtaaagt atcaataccc ctcttataag
     1621 tatctttctt ttttatttgt gcctgcattg ttttattaag ttcagaccat tccttattca
     1681 tattcaatcc tctctcaaat tctgatttta tcgccccata caaactggaa tttgtcttac
     1741 aacaatttat atatgcataa aaagcaatat atttatgaaa acaccctaaa tccaaaattt
     1801 actcaattca ttttttcctt acataaaatg ttctcgtata tttcacttcc tcaccaagtg
     1861 tttcccctat caaaacttca aaatcattga tatcaaattc tggaaagaat gtatctccat
     1921 cttcaatatt taaatctact tctgtgatat acattttatc tactatttgc aaagcttcct
     1981 taaatagtcc atatccacca gatatgtata catctcgtcc tttagccaat aataatgcat
     2041 cttctaatga tttaactgaa actaaattat ctccttgata ctctgttgtg gtggaaacaa
     2101 caatattcat tctattaggc aacggatgac cgatttcttc ataagacttt cgccccataa
     2161 taaccacatt acccgttgtt aactctctaa attgcttttg ttctcccttt attttccatg
     2221 gtatattacc attcttgcct ataacattat tctttgacct cgcaacaatc aaacctatca
     2281 tctatttcat cctccataat atgatgtcgt acctgtgatt ccatcaagtg caccacatct
     2341 taacaaactc aaaattatct cgaactaacc agctcgacaa attccttttt tgtcttcatc
     2401 tgcattatat catattgtta taagcattgc catttctttt ctgcttttga aaagttactt
     2461 atcagcctat gttttgtaaa gggtgatatt cagcatttct cacgcaacaa gcccttgaca
     2521 aaccatagac taataagttt taaggtaatc aagcagaaat gaaatagtct gctgtttcac
     2581 ggcaacaaaa aagagatacc aacacactta tgtagtatct cttagtctag tagcaatatt
     2641 cttttgtttg caagcaggta aacataggct gtttcttaat atctttttgt ttgggaaaca
     2701 cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac
     2761 catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg
     2821 ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt
     2881 aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg
     2941 aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct
     3001 tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc
     3061 acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag
     3121 ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct
     3181 gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt
     3241 ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca
     3301 ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct
     3361 gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta
     3421 atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct
     3481 gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg
     3541 atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga
     3601 ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca
     3661 ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa
     3721 acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc
     3781 aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg
     3841 taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta
     3901 acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca
     3961 aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa
     4021 gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct
     4081 agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct
     4141 gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag
     4201 aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca
     4261 gacaattaat aaaagcttga tgtgttgt
//
LOCUS       DAJSVD010000172         4282 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSVD010000172 DAJSVD010000000
VERSION     DAJSVD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.176"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(37..1257)
                     /locus_tag="ON498_002775"
     CDS             complement(37..1257)
                     /locus_tag="ON498_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR3940982.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ON498_002776"
     CDS             complement(1277..1888)
                     /locus_tag="ON498_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3940983.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ON498_002777"
     CDS             complement(1931..2863)
                     /locus_tag="ON498_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3940984.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ON498_002778"
     CDS             complement(3284..4144)
                     /locus_tag="ON498_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3940985.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAJSVD010000173         4266 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSVD010000173 DAJSVD010000000
VERSION     DAJSVD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.177"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(128..1894)
                     /locus_tag="ON498_002779"
     CDS             complement(128..1894)
                     /locus_tag="ON498_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940986.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1854..3608)
                     /locus_tag="ON498_002780"
     CDS             complement(1854..3608)
                     /locus_tag="ON498_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3940987.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3718..>4266)
                     /locus_tag="ON498_002781"
     CDS             complement(3718..>4266)
                     /locus_tag="ON498_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR3940988.1"
                     /translation="FIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFV
                     YISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIP
                     QVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVL
                     VVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1445 a          806 c          599 g         1416 t
ORIGIN      
        1 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
       61 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
      121 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
      181 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
      241 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
      301 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
      361 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
      421 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
      481 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
      541 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
      601 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
      661 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
      721 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
      781 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
      841 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
      901 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
      961 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
     1021 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
     1081 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
     1141 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
     1201 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
     1261 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
     1321 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
     1381 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
     1441 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
     1501 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
     1561 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
     1621 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
     1681 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
     1741 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
     1801 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
     1861 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
     1921 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
     1981 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
     2041 actgtttttt cttttatcag atgttgaaaa gccttttgta gttcatactc attatctgga
     2101 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
     2161 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
     2221 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
     2281 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
     2341 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
     2401 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
     2461 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
     2521 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
     2581 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
     2641 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
     2701 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
     2761 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
     2821 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
     2881 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
     2941 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
     3001 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
     3061 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
     3121 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
     3181 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
     3241 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
     3301 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
     3361 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
     3421 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
     3481 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
     3541 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
     3601 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
     3661 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
     3721 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
     3781 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
     3841 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
     3901 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
     3961 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
     4021 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
     4081 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
     4141 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
     4201 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
     4261 aataaa
//
LOCUS       DAJSVD010000174         4229 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSVD010000174 DAJSVD010000000
VERSION     DAJSVD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.178"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            14..175
                     /locus_tag="ON498_002782"
     CDS             14..175
                     /locus_tag="ON498_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3940989.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            172..477
                     /locus_tag="ON498_002783"
     CDS             172..477
                     /locus_tag="ON498_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940990.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            477..833
                     /locus_tag="ON498_002784"
     CDS             477..833
                     /locus_tag="ON498_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR3940991.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            820..1038
                     /locus_tag="ON498_002785"
     CDS             820..1038
                     /locus_tag="ON498_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940992.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1059..1148
                     /locus_tag="ON498_002786"
                     /pseudo
     CDS             1059..1148
                     /locus_tag="ON498_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1223..1690
                     /locus_tag="ON498_002787"
     CDS             1223..1690
                     /locus_tag="ON498_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCR3940993.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            1965..2201
                     /locus_tag="ON498_002788"
     CDS             1965..2201
                     /locus_tag="ON498_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940994.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            2225..2566
                     /locus_tag="ON498_002789"
     CDS             2225..2566
                     /locus_tag="ON498_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940995.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(2559..2750)
                     /locus_tag="ON498_002790"
     CDS             complement(2559..2750)
                     /locus_tag="ON498_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR3940996.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            2960..3607
                     /locus_tag="ON498_002791"
     CDS             2960..3607
                     /locus_tag="ON498_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3940997.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            3867..4211
                     /locus_tag="ON498_002792"
     CDS             3867..4211
                     /locus_tag="ON498_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3940998.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1574 a          582 c          882 g         1191 t
ORIGIN      
        1 gaggtggtcc atcatgacaa cagaagaagt gattcaaatg cgtattcgaa acattcagcg
       61 tgaaattgac gatctagaac gaacaaaggc agtgatggtc aatgaaacgg cgagaaaggc
      121 aatcgatttg cacatagaga acttaagaag ggaaattcgt agattggagg aatgagcgtg
      181 gataagaaag cgacaatgaa acgaattgct gaattaacca agtcagaatc ttggcaggaa
      241 gacaaagaaa tagttgcaga agtccaaaag ctcggtaaat caatgtggac tgaaaagcct
      301 aaacggaaaa cgccgagaaa aattgcaatc tggcatggtg atcgaattct agtaacaggt
      361 acagctgaac agttatctga aattactgga ttaagcaaaa acattatctg ggatagagct
      421 aggagcttat ggattgattc aaaaggacga cagtttaggt atgtggagga gagataatgg
      481 atctcattac acaatacagt gacatcatcc tcaagaaaat catgatgaag attcagaaag
      541 acaaaaaatc aaaagaacga gctgaattag ttaagttaga aatggctgaa acaggagcag
      601 gagtgcgaag tagcaggcat tggaaagcag cagcaaacat tgaattttat tacaacgaaa
      661 ttcaaaaagg gttcgatcag atgcgtgagc tggatcggca aacaaattgg agcaagaaac
      721 ttcatcaaga tcgtttcaaa tttgtagaga agtataaaga aatattagaa gagtatttga
      781 ggaggacagc aaatgataaa aaaactcgtt caattcagca tggatttata tgatatcgaa
      841 tcaggagcaa cactatctgt ggaatcggac catctaatca taaattttgg tggaaagcgc
      901 cagattattt tgtgggtagt tgatgatgta ctgtttccag aaattgttca tgatttcgaa
      961 gaatcaaaag cggttgagtt tgaaatagtg aaaaaagtaa tggaattgat tgaaaaatac
     1021 gaggaggaca gcgaatgata ccgaagttta gagcgtggga tagagaaaaa gacagaatga
     1081 aggcagtagc tttggttgaa tttgctgagg cgtataaatc aggagaactc atttcacgta
     1141 caaaataaaa aaagtcggat tcctccgact atgaataata tttccgacat aagtattata
     1201 tcataattgg gggaatcaga ggatggtact tttcgatgta aagaaatatg agacaccaag
     1261 cgcgaaagat gttgatatgg aacgcacaaa acataatgtc gctgtgtttc tttcagcata
     1321 tctatcagct agatgtagag tagggcaacc tcgagaacca aaagtgacag catcttattc
     1381 cttggttcca ccttctacag ctgatcatat atttgaagca gaaagaatgc tgatcgataa
     1441 agaagaagca caggaagaat ttgagtatct gcataaattg tttgttcgag gctattcagc
     1501 gatacagcat ccgcataagc ctgatgtgac tgaaaggcgc aagaagatat tctatgatcg
     1561 ttatatcaat ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc
     1621 ggttaaagta gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc
     1681 tttcaagtag ccagttttta cactttttat acccttttat taccaatttg gtttccattt
     1741 tatacctttt ttataccaat cacttaccta ttcaacgttg tattatgata gtgtcgaaag
     1801 attaggaaac aggatcgaca aaataaaatg taagggagga aatctccctc atcgtttaat
     1861 taagcttcga taggcagcag cggaaatatt aagaataagg atgtgaattt caactccttc
     1921 taaattgttc ttattatcta tcatccgttg ctgtctatta atttatgtat tggagggtat
     1981 ataaaatgaa gatcgcttcc gaaaaaattg atcaactaac aattttagta aaccaaagaa
     2041 tcgaagatat tatcaaagaa ttcgaaggtg aaaagattaa gaacataaag cttactttaa
     2101 tggatcataa gttcgaatta aattcgacaa aaaggaagat cgttgagctt atagaagaag
     2161 aaatagctct ccagatcaat gagcaacaat tagctaatta atttatgtat tggaggggaa
     2221 atgaatggaa gtcgaacaaa taaagtatca aggtatggat cgggaacggt tgattgatat
     2281 tattgagcaa caacgtttag aattgatcca aaaagaagct gtatgtaaag agtacaaaaa
     2341 acatttggaa caagtaattg agtatcattc agtagaaaag tacagatcag tggtgcaaaa
     2401 aaatagagca acgagtcctg attctcgtca agatgtatcg attagtatca agacgccagt
     2461 atttaattct gttaaatcag aaaagctaga aagtatctca actgctttgg tagaaaaagc
     2521 agctgaaatg aactccctag caaatgaagt tagtcgtctt atttagtcat atcgataact
     2581 tgagagtctt tggtgattcc gttttttata gaattaatgg tttgttcagc ggaatattta
     2641 gtcaagtatg tttcgctagt tgctactact tcattgttat tggacttaat aacgaagtaa
     2701 tattggccat tagttgcttc tcttataaca aagtacaaat ttttcaccac ctataattta
     2761 tttcagcgga ccactcgctg ataattaaaa ttataagcta tgtataaaat ttcacaatat
     2821 caatttgtcg ctgtggcgga aagggtagac gcaataaagg atgaatggga aaagtcaatg
     2881 agtaattatc tgaaatttac aagtcccctg tttagttatc taagattatt tgataatatt
     2941 atttctgagg tgatagaaaa tggtgggcgt agttaaattt cttgggttta catttgatgg
     3001 atatggtacg ttaggcgaat gggtaggtat tattttgata gtgattacaa ttcaacaaac
     3061 atcaaagtac tactggtatt ctaatagttc taaaattgta tcaagaataa ctgaaggaat
     3121 aaccaaaaaa gatattactg tcgtcggtaa atatgggttt actataactt tattaaatga
     3181 agggaaatct gaaaccgcaa taaagttttg cgggattata ggtaaatata acattttcca
     3241 acgttttatc agaaagcatt ggtacaaata tatttacaaa taccctgtag ttaaaaagct
     3301 tattgaaaaa tatgtggacc acgaaatatc ttatctcaat ccaacagacc ttattaatca
     3361 agaattccag aatattggat acttagaaaa aggtctaaaa ataccaattg taaatgaaag
     3421 tttagttact gcaatggtag ctaacataaa gaacagcaag aaattagaaa agagatataa
     3481 aaaatgtaaa aaattgaaat tttccttttt attcttagaa tataatggca aagaatatta
     3541 tcaagatatt tatgttttgc gaggctcgaa cttatataaa gacattaaaa ataagatttc
     3601 agtatgagat tttttaaaat tcgtagatca ctctttgagt ggtcttttta ttttgcgtaa
     3661 aggaggcacg acgatgtata gaccacaata cttagaacag aagcatgaag taatcactgt
     3721 gcaaaacggt aacggtgaga tagtacgaaa gtatagaaga ccaataaaga gcgatacata
     3781 taaacggaag gaaagcaatg aagttattcc attgtatggc aaaagaatag ctaagtatta
     3841 aataagattg cgaaaggaga tgggacatga ccgaggaatt ctatagatgg ttattacagt
     3901 tgataagaga agatcgtttg gttaagttct atcagtctcc taaatggcgc aggcttagag
     3961 agaaagcgat gaaacgagat cactatgaat gccaagagtg tagaagacta ggtaagtatc
     4021 atagagtaga gaacgttcat catataaagg aagtcaagga tagacctgac ttagctttag
     4081 atttagataa tcttatttgt ttatgtgttg aacatcataa tgaagttcat ggcagatatc
     4141 ttacagcgtt agataaacaa gagaagaaga tagaaagctt cgctaacttc gatgcaagtg
     4201 aaaggtggta agtgcatgat catcaatga
//
LOCUS       DAJSVD010000175         4228 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSVD010000175 DAJSVD010000000
VERSION     DAJSVD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.179"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            493..1461
                     /locus_tag="ON498_002793"
     CDS             493..1461
                     /locus_tag="ON498_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3940999.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1472..2287
                     /locus_tag="ON498_002794"
     CDS             1472..2287
                     /locus_tag="ON498_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR3941000.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2355..3065
                     /locus_tag="ON498_002795"
     CDS             2355..3065
                     /locus_tag="ON498_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3941001.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3101..>4228
                     /locus_tag="ON498_002796"
     CDS             3101..>4228
                     /locus_tag="ON498_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3941002.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1413 a          633 c          894 g         1288 t
ORIGIN      
        1 gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa tcagttttaa
       61 gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt ttaagccatt
      121 ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact ccagtagttt
      181 cctaattcaa ctgtgtgcaa aaacgatttc ttctaaacaa atcaagacaa acttataaac
      241 cttcatccca gcaccataat tatttaaatc ataggcattt ttcgaaatta gtatatgtac
      301 tcaagtttca ctgcagatta tttatatttt ttagagaaat aaatttttcc tattgacaaa
      361 cacaaagaaa gcggatacaa tgcaattatg tcacatgacg ttatatgacg ttatgtgata
      421 tctttacatt taatcagtta cttttatttt ggataaaatt aacattttta aaattttaag
      481 gaggaaatgt tgatggatgt agcaaaaacg gttaacgcaa ttgtagaaaa aaatgatgga
      541 gagatttctg gaatttattt ggtggcttgt ggcggttcgc tggtagatat gtatgtctca
      601 gattattttc ttaaagccga agcatcgaag tgtattacag gcttatatac agcgaacgaa
      661 tttaaccatg taaccccaaa gcaattagat gataaatctg ttgtcattct atgttctcat
      721 ggaggaaata cagcagaaac ggtaaaagca ggagaagtcg ctcaagctag aggagctgtg
      781 actgttggtc tgactcataa taaaactgcg gatttattaa aggtttctga tcatagtttc
      841 ctttacgaat ggggagatga tgcaaaaata gtcaataatc ctatggccat cattcttgat
      901 ttaactgtat ctttggtcaa tgttaccgaa acatttgaac attacggtaa attcagagaa
      961 ggcatgaagg ttatcgatac cgtaattgaa aatggcatta agcaggtgca ggatcgtatt
     1021 aaagtgtttg ctgataagta caaagatgaa acaatgtttt atatcttagg tagtggacca
     1081 tcatatggac atgcttatgg tttttcaatt tgctctttga tggaaatgca atggcttgat
     1141 tcaacttctg ttcattctgg agaattcttc catggacctt ttgaagtaac agacaaagat
     1201 acgaatttta ttctcttgat gagcattgga cgcactcgtg ctttagatga acgggcaaaa
     1261 cgattccttg ataaatatgc agaaagagta gaaatcgttg atgcaaagga actaggattg
     1321 gatatgatat cagatgaagt ttctgaattc ttcaatccca ttttattcta cagtattctt
     1381 tcagaatatc gttcagcttt agcagatgta cgcaatcatg atttagatgt tcgtcgctat
     1441 atggggaaag tggagtacta ggaggattaa aatgaaggta ttaggcttag gtgataacgt
     1501 cgtcgataaa tatgaaaatc tgggaattat gtatcctgga ggaaattcat tgaattttgc
     1561 agtttttgca aaaaaactgg gacatgaaag tagctttatg ggagtttttg gctcagatga
     1621 ggaggcagct catgttttat cagcaataaa agaaatagga ctggacaaca gtcactctcg
     1681 ctttgaaaaa ggcgagaatg gctgtgccag agtgaagatt gatgagggcg accggatttt
     1741 ccttggcagc aattcgggag gtgttactcg tgaatacccg attcagctta ctgaagaaga
     1801 cagagaatat ttaaaccagt ttgaattgat tcatatggga ttatatagcc atgtcaatca
     1861 tctattagaa gagttacaaa atgtttctgg aaagatttct tatgattttt ctgacgactt
     1921 tacggaagaa gaggttcagc gggcaattga taaagttgat tttggcttct tttctataga
     1981 agacttttcc gatgaagaag taaagcagtt tttgatgaag catttttgtg agcgaaatga
     2041 tgtattgatt gcaacccgag gagaaaaaac ggctattggt tatgatggag caaagtttta
     2101 tgaagtgaac cccgtgctag aagaaccagt tgatacaatg gcagcgggag attctttctt
     2161 aacagctttc ctactttatt ttctcgaagg ggacgatata gtcaccgcga tgaaaaaggg
     2221 gaatgaattt gctgggaagt cttgtctggt tgacggttcg ttcggctttg gtattcatta
     2281 cgattgagct tgttttaaat tcctgcattt gataacctaa agaaagaaat cccggtaaag
     2341 tggaggttaa aaaaatgctg gatacaaatg cacaattacc attgtactta caattaaaag
     2401 aaatattaaa aaataaaatc aatacagggg agtatcccga gaatacgaaa attcccgctg
     2461 aatcagagct gatagaaagt tatgaggtca gccgaatcac agtgagaaaa gcagtagaag
     2521 aactggtaga agaagggtac ctacttaaaa aacaagggaa aggaactttt gtcaaccggc
     2581 ataaagtttt tcgtaaaatt gaatatatta tgggtttcag tgagagttgt aaatctaatg
     2641 gttttacacc gactagcatt gttttggaac gcaagatcat tccagcaact gaagggatgg
     2701 caaaaaaact cgatatcgct caaggtgatg atgttttata tatccagaga aagcgaatgg
     2761 cggataacat ccccattatg ttagaaaaca attatttttc ttatgaaaga tatagtttct
     2821 taatggagga agagctgact gattctttgt atgaagtact taaagtccaa ggtaccttag
     2881 ccatcaatcc tggggaaact gcacttgaaa ttgtcacagc tgatgaccaa attgccaaaa
     2941 taatggaagt agctgttggg acgccctttt tctttatgga tactttaatc aacgatcaaa
     3001 acaacatacc aattcatgta ggtaatcaat attacctggg agaagtatat cgtttttact
     3061 tatgatttcc tgtttggatt tcaaggagta gattttattt atgaagcaaa tattgcggtc
     3121 aaaaatgatt tttgatggag agcagccacc tttcaaaggt tatattgtaa tcaatgaagg
     3181 gatcattgaa aaggtggaaa acaactggaa ttacagtgat ctattaaatg aaaacacgga
     3241 attaattgct tatgatgatc aattcgttat gccagcaatt catgataatc atgtattttt
     3301 cagtggttat ttggctatga atgctggcat agatttaagc gatacatctt cagctaacga
     3361 ggctgtagag cgtattaaag ctgtaatgaa ccataaaaaa gcagatgaac caatttatgc
     3421 tcatggctgg gatgctgccc tgtggaaaac tgacccatca gaagagctat tgaatgaaat
     3481 aaaccacttt ggtccaatca cagcgatcga caaaaatcgc agccattgtt ggatgaatgc
     3541 tattgctaag cagacttatg gttttacaga aaaagaaacc agtgctgaaa gtcgagtatt
     3601 attaattaag gaaatgctag caaacaaaaa gcttttagca gaggtttacg ctgaatttga
     3661 agcattactg ctttcaaaag gtgttatatc aatgaaagaa atcgtttttg atgacgcaga
     3721 atttctttct aatatacctc agaggaaaat acttagtaac ctatatgtgc aggcagtcca
     3781 agaacctcta cagcatgaac ttctatatga gtataaggaa aaagttttcc ctgaaaaggt
     3841 ccgctttggt ggagtgaaga tcatggtaga tggtgttgtt gcggatagaa gtggagatat
     3901 ttacggtgtt tatcagtcag gagtagcttc acccgagatt gactatgagg caattaaagc
     3961 agaagttgct gcattcaatg aagtagagat tccttgctgt ttgacaacgg aaggagataa
     4021 agcgggttat aaagctgcgt caattcttgt cgaatttgga aagcggttac ctgaaggcgt
     4081 ttttaattcg attagtgatt tggaaatggt tactcaaaag acagcagaaa aaatgaacga
     4141 aggacaagta gtagcagagg tatatccaca aattctcggc ttgaatcaaa cgttagaaga
     4201 ggcttacatg tcaactgtta taaaaaat
//
LOCUS       DAJSVD010000176         4157 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSVD010000176 DAJSVD010000000
VERSION     DAJSVD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.180"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            179..775
                     /locus_tag="ON498_002797"
     CDS             179..775
                     /locus_tag="ON498_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3941003.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="ON498_002798"
     CDS             899..2698
                     /locus_tag="ON498_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3941004.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="ON498_002799"
     CDS             complement(2976..3188)
                     /locus_tag="ON498_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941005.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="ON498_002800"
     CDS             3650..3799
                     /locus_tag="ON498_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941006.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4052..>4157)
                     /locus_tag="ON498_002801"
     CDS             complement(4052..>4157)
                     /locus_tag="ON498_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3941007.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1493 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DAJSVD010000177         4103 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSVD010000177 DAJSVD010000000
VERSION     DAJSVD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.181"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            52..1353
                     /locus_tag="ON498_002802"
     CDS             52..1353
                     /locus_tag="ON498_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3941008.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="ON498_002803"
     CDS             1380..1640
                     /locus_tag="ON498_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3941009.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="ON498_002804"
     CDS             complement(2639..3874)
                     /locus_tag="ON498_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3941010.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1167 a          664 c          862 g         1410 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ctttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaagag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctgccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccataat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaaca tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccagactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgta acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggaaaaaa tggaaatttt atcagcaatg gatttagaat
     1921 tagtagatct actaccaaag tgatcaagtc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgtgagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgtgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagtgt atcgttgctg acgacataga acattctgac atgatctact caatatgtgg
     2161 ggattgctgg cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaggggacta gcggcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggata gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 ccagataaat acgagaagtt gtg
//
LOCUS       DAJSVD010000178         4043 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSVD010000178 DAJSVD010000000
VERSION     DAJSVD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.182"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(153..1658)
                     /locus_tag="ON498_002805"
     CDS             complement(153..1658)
                     /locus_tag="ON498_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3941011.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="ON498_002806"
     CDS             complement(1655..2341)
                     /locus_tag="ON498_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3941012.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ON498_002807"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ON498_002807"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3941013.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1116 a          856 c          691 g         1380 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgcat tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat att
//
LOCUS       DAJSVD010000179         3971 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSVD010000179 DAJSVD010000000
VERSION     DAJSVD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.183"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            10..615
                     /locus_tag="ON498_002808"
     CDS             10..615
                     /locus_tag="ON498_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3941014.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            635..997
                     /locus_tag="ON498_002809"
     CDS             635..997
                     /locus_tag="ON498_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941015.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            1000..1182
                     /locus_tag="ON498_002810"
     CDS             1000..1182
                     /locus_tag="ON498_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941016.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1199..>3971
                     /locus_tag="ON498_002811"
     CDS             1199..>3971
                     /locus_tag="ON498_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3941017.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSI"
BASE COUNT         1427 a          594 c          859 g         1091 t
ORIGIN      
        1 ggaacaacaa tggctaaaac aacaacagta acaacttttg acaatataag tattaaaaga
       61 atttctttta attttaagaa tgcaacaaat gcaatttcaa ctgattgtaa tggacaatta
      121 gatggcgaaa cagaaatgca gagaattgta aaaaaatgcg gttcaacaga agtaaaatcg
      181 aaatctaaac caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat
      241 cgacgcttca atgggttgaa acaagatgaa cgtattaaat cgggaattta ctcttatggt
      301 cctgattccg taggggaaga tttctctctt gctgcagaga tcgtggatga tttcgaagaa
      361 aaaaataagc tgattggtat gttagcatgc acttcgaata caggattaac attctctatt
      421 gaaaatggtg cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa
      481 tttggtaaat tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa
      541 tggatgacga atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc
      601 actcaatcac agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga
      661 actaaacgat ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat
      721 gatcaataga gattttaaca cagacgagtt tgcaaaaatg actgtgggaa aaggatccat
      781 ggatatttct gttattcaag gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat
      841 gactgattat atttcattcg atgaatttat cgataaatgg gattttgata tggctactgc
      901 cagctatatt tatcaattga tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt
      961 tgaaaaagca aataaggaaa aaaagcttca aaagtaaaaa tgccaaagct cttagttgaa
     1021 acgtgggtcg atgtctattc gatgttgacc gacgtttttt ctatgccttc agatttggtt
     1081 ttaagcgata tctgtttaga tgacattttg caaatggctt acaacaagag tgcttatgaa
     1141 ggatggaaaa actatgcaat aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat
     1201 ggctaaaaag agaacagaag cagaagtaac attcatagct aacgatgacg gattgaaatc
     1261 tacgttaaaa gaaatcagtg ctgaattaac taaaaataga gcagaattaa aactagaaca
     1321 agctcaatta caacagactg gttctgaatc agacaagtta ggaagtaaat tatcttcttt
     1381 agaaaagcag tatgaattac aaagtcaaaa agttgaagta actagccaac gtttagccaa
     1441 tgccaaaaaa tattatggag aaaattccac cgaagttcag aaacttgaga gagaactgat
     1501 taaccaacaa acagcgcaac aacgtttgtc aaacgaaatt gataaaacga gtaatgcact
     1561 agctcaagca aaaggcgaaa tacagacgta cgagtctaca atgcaacagt tggatagtga
     1621 acaaaaaaat gttcaagcta gtgcttctct gattgaatcc gaatacaaaa aatggcaagc
     1681 aactgctggt caatcagctt ctgaatccga gaaattagcg aaagcccaag aatatgtttc
     1741 tcaacaatct gaaaatgcag agaaaacgat agatatcctg agacgacagt tagaagctac
     1801 acagtctgag tttggcgcta catccacaga agcaatgcag atggaggcga agcttaatga
     1861 tgctgaacgt gaatttgaag agttaggaca agctgctaaa aatgtagata caactaactt
     1921 ggacgatatc ggaagcaaaa tagatatgaa taatctaatg gaagcttctg acgttttaag
     1981 cgacattggc gataagctta cagaattagg gaaacaagca gtggactctg ctaatagtgt
     2041 aggtagttcc cagagtaaaa tacaagctaa ttttggtttg actaaacaag aggctgaaga
     2101 attaacgaat gtagccagag acatttatta taaaggtttt ggagaatcgt tagatcagtc
     2161 cacagatgca ttgattttgg taaagcgtaa tttaggcgat ttaaataatc aagatttaca
     2221 aaatatcacg gaacaagcta tggtcctaga aaacaccatg ggcgctgata tggatgaaac
     2281 gttacgtggt gtaaatggct taatggtcaa tttcggcttg agcgctcaag atgcaatgga
     2341 tttaatggtt tcgggtactc aaaacggttt agataaaacg cacgaattag gcgacaatat
     2401 ggcagaatat agccaattat ggagtcaaat gggatattca gctgatgaaa cgttcggaat
     2461 gcttcaaaat ggtttagatg cgggtgctta taaccttgat aaagtcaatg acttagttaa
     2521 ggaaatggga atatcgttaa cagatggtcg atttgagcaa aacatggata tgtttagtga
     2581 aagtactaga aaagcttttg aagagtggaa aaatggcgga ggaacacaaa aagacgttat
     2641 taattccatg attcaagatt ttagcaatat ggatggtcaa tacgaccaat taaataaagc
     2701 ttctacaatt tggtctgcac ttggcgaaga taacgcgatg aaagttgtcc aatctttgac
     2761 tgatgttaac catacatttg atgatgttag tggatctgca caaaaaatga atgaagattc
     2821 tactactccg ttacaagaat taaacggaaa aatagctgaa ttaaaggatt cattagctcc
     2881 tataggcaac acaatcatag atgcactcga accagtaatt gattttctag gaaagatggc
     2941 tgatgcgttt aataatcttc cacaaccagt acaggattat gccgtagcga ttggcggatt
     3001 gactgctgca tttactttat taatgccaat aatagttggc ttcatggctc taggtggtcc
     3061 tactacatta ataataggag cagttattac tgttattgct ggagttatag caattataaa
     3121 aaactggggc gcaattactg actggtttaa gggaatatgg agtaaattca ctgattggtt
     3181 gggtggtact tgggaaagta taaaagaagg tgcctcatca gtttgggatg gagttaaaga
     3241 aacctggtct ggatttgtag attgggttca agatatttgg caaggagttt ctgattggtt
     3301 tggagagcta tggagcggat tagttgaagg agcttccaac atctggcaag gagtccaaga
     3361 gacttggcaa gcattcgttg attgggtttc aaatatttgg aacggagtca aagaagtatg
     3421 gtcgattatt tgggcagaca ttgtaggaat tgttcaaata ccatggacct taataacgtc
     3481 attgattcaa gccggtatta atattatcgt gggtattttt gatgtagctg gacagttatt
     3541 aggcgcagct tggcaagctg tttggacacc tatttctgat ttccttaaaa acacttggga
     3601 tactatgaca caatgggtaa gcatcgcttg gaatggaatt gtaactacat tccatactat
     3661 atttgatcca gtagtggcat ggtggaatgg tatatggaca gctattagta ctacggcttc
     3721 aaatatttgg aattcaatta gtgcaacagc ttctagtatt tggaacagta tcaagaatac
     3781 aatcactagc ttggtacaag cagctgctac agtaattcaa aatatttggt caactgtatc
     3841 tagttggtta ggtggaattt ggaattcaat cagctctaca gcatcaaata tctggaatag
     3901 cgtgactagt agtataagca atgctataaa cgcagctaaa agtgccattc aaagtgtttg
     3961 gaatagtata t
//
LOCUS       DAJSVD010000180         3766 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSVD010000180 DAJSVD010000000
VERSION     DAJSVD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.184"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..566
                     /locus_tag="ON498_002812"
     CDS             <1..566
                     /locus_tag="ON498_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941018.1"
                     /translation="YLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTV
                     EGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYE
                     YYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASL
                     RYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            610..879
                     /locus_tag="ON498_002813"
     CDS             610..879
                     /locus_tag="ON498_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941019.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1111..1407
                     /locus_tag="ON498_002814"
     CDS             1111..1407
                     /locus_tag="ON498_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941020.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1409..1831
                     /locus_tag="ON498_002815"
     CDS             1409..1831
                     /locus_tag="ON498_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941021.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1850..3442
                     /locus_tag="ON498_002816"
     CDS             1850..3442
                     /locus_tag="ON498_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941022.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3463..>3766
                     /locus_tag="ON498_002817"
     CDS             3463..>3766
                     /locus_tag="ON498_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941023.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1491 a          537 c          738 g         1000 t
ORIGIN      
        1 gttacttact cgtccaattt atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt
       61 tattggactt acttatctta ggcagtatgt taacgactct aacagatcgg ttcgaacaat
      121 ctgaaaaaac ggtagagggg ataaagaact tcttaacgac gattgatcaa tttataaatg
      181 gatatatgta tttgtttgaa gtaggcttta ttctcacttt gtttggttgt gtatttataa
      241 aactcattaa aagatcaatc gaaaaagaaa tggttattga tctaattccg acgctgagcg
      301 gctatgagta ttacgttgat tttggaaagt atgaggagtt gatccatacg gatatcgttc
      361 acacgagtat ccatgaagta accgttaagg gcatcagatc tactttaaaa gatggacagg
      421 agacgaaaac aatcgaccga agaaaagagg tctacaattt ttataaagtt gaagcaagtc
      481 tacgatatgg cattgggaaa ttgaggttag atgggagtaa tattcaacaa aacaaaccat
      541 atgttttagg aaaagtggtg atttagaact tcctatgaaa gatgaataaa taaggagaat
      601 cttttgaaaa tgattcttct ttacagaatt gtgcttatag ttggcacgga gttaaaaatc
      661 ttaaagaagg agcagttatt caaagtgaaa gaacatcata atcagttact atgtattaaa
      721 acgggtacga tgaacattca ataccgaaca gaatatttag aattaacgaa tcaaactgat
      781 tttttgtcga ttcgagagca acataagcaa gaaaaattaa tggctactta tagccaattg
      841 attgatgagt gggaacaatt caaggaatct aaaacataaa attccattta aacaccttat
      901 tttttaaaga gcataaggaa acgtgacccc acttttaaaa aaagtggttg gataggctgt
      961 gcctatcacg tttctgaaaa aaataaatga ccccaaaata ccaatatata ggatagtaca
     1021 aggatagcaa aggatagtaa aaggatagag gtaggatagc gaaaaggata ggatcctttt
     1081 cactatccta tattatattg gaggatattt atgaatgtta aaattagaga tttagatcca
     1141 aaattcattt ctgaaattga tcgaagatgc gttgaactaa gtaatcgtac aggaaataaa
     1201 tggagtcgta atgattattt aaagctactc gttgaaaatg attttgatcg tcctttgatg
     1261 gaatacaaac aagaaaagtt tgaccagtta ttagaaaagt tttcagaaat tcaaagttat
     1321 aacacaaaga tattagaaga atatgttagc caaaataatc gtattataga aatcttgatt
     1381 gagcaaaata gaggaagtga actttgatat ggcaaaggtt atagacaaac acatttactt
     1441 tcaccaaaca catatcgatg tattggatga aactatttct actcatgcag aggttaaaaa
     1501 ctatagtgaa gctgttcgct ttttatgtat gaactatgat ccagaagtgg agaaaaaagt
     1561 aaattccaat caagcaaagt tgaatggaat gagtaaagaa atttctattt taaccgaaat
     1621 ggtttcagaa tttgcggatc ttcatttgaa agatacagga atattaattg gtaaaaattc
     1681 aactgtttat caagaagcaa aagaacgagt ggaaggaaaa attaaatcca atcaaacaaa
     1741 aaaatattcc cctcagttga agaaacctgt gaatgaatct gttcatgcca gagtagatcc
     1801 atttgatcca acgaagattc taaaaagata aaaatgaagg aggataataa tgactcaatc
     1861 gccttcagtt attctcactt gtcaatttac tttgccgaat aaaaagtcat tttctactta
     1921 tattgactat attacaaggg aacaggctct tgaagaaaag gaacgacgta cgcccgaaga
     1981 agaacaggaa cttcaacgaa tcaagaatgc tttagaggag ttttatattc ctgaaggaga
     2041 aactttttcg gagagtaaga cgggtaaaga attcagtgaa acgtcgaatg aagctcaatc
     2101 gttgatggaa catggaatca attttgacaa gttagaggaa caagatttta ccaattatct
     2161 ttcttatatg acacgtcact atgcgttaga acaaaagaaa gaactgacgc aaagtgaaca
     2221 aaaagaatac caacgcttga accaagcaat gaaaaaatat caaacagaac cccataaaaa
     2281 agcttcagga aatcttcctg gagttttttc tatagcttca gacgaagtga aaggaagtga
     2341 tctgaaggag attaagcaaa tcttccaaag aggacaacaa aaaggctcaa tcatttatca
     2401 agatgttgtt tctcatgata attcgtatct tgaaaaaata ggattgtacg atcccaaaac
     2461 agatacttta gatgaagaag ggttaaaagc agctggaaga aaaatgatgg agacattatt
     2521 cgagaaagaa caattgaatg aaacaggtta ctggatggca acgattcacc gtaacactaa
     2581 acatatccat attcattttg cagttgttga aaaagaaaat acgcgtaaac cttacacgga
     2641 ggttgaaaat ggtgtgagtt atatcgtacc gaaaggtaag agaagacaag caacattaga
     2701 cgcaatgaag accaattatg tacatgagtt agaacgtttt tcttatgaaa aggatcgaat
     2761 gattggtatg gagaaacgaa atttattgac gcggaaaagt gatttgcgta acaccttaac
     2821 aaaagtggta aaagagccag cgcgttacga tcgacacgcg atgaacttat taaatgaagt
     2881 atataaaacc ctacctgaca aacgttcgga atggaattac ggggatgaaa aacgaaccaa
     2941 actttcacca tacacaagag agaaactcga tgagttaacc aaatatgttt taacaaaaga
     3001 acctgaatat caagaatata tacagatttc aaaagcattg aaagaagaag caaaaagtct
     3061 ttatggggaa agcaagagag aatcaaagga tgcagagaaa aatgctctct ttgatttaaa
     3121 aaagcgcaca ggaaatgcca tcctaacaga gctgaagaga caggatagtc aaataaaacc
     3181 attaatagaa agtgttgatc agatgcaatc tatttacaat gaacgatcct ttcgaacgct
     3241 taaacaattg ccaacgaatg aatacaaata tagttacgaa gaattcatga agcgcaggaa
     3301 acaattccaa caaagagttg tcacgcagcg gagtttgcgt caattaaatc gaacgattaa
     3361 tgcccatgaa gaaaaatacc acgcaagtaa agcatacgaa gagcaacaac gaagaattca
     3421 aatggaacaa gaaagacagt aaagacagta ggaggaatag caatgaaaca atatttagat
     3481 caatggaaag ttatagaagg atcattaaga gaagaacgaa tcgaacaact tcctgattgt
     3541 ttagaaaaag agcatttgtt ccagataaga gaaatgttga gaaacgaaca gtttgatcca
     3601 aatcaatttt tagtcgttga gtacccagct actggtgtgt attgttgtaa tcatgtgaat
     3661 ggtgaaaaat attttatcat tcaagaatat gagggcaagt tagcacctta ttatactact
     3721 tgggaaatga atgaagaggg aatcaataac tttccatgta aaagca
//
LOCUS       DAJSVD010000181         3742 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSVD010000181 DAJSVD010000000
VERSION     DAJSVD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.185"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            75..527
                     /locus_tag="ON498_002818"
     CDS             75..527
                     /locus_tag="ON498_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941024.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            541..1230
                     /locus_tag="ON498_002819"
     CDS             541..1230
                     /locus_tag="ON498_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941025.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1355..3706
                     /locus_tag="ON498_002820"
     CDS             1355..3706
                     /locus_tag="ON498_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR3941026.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1271 a          491 c          774 g         1206 t
ORIGIN      
        1 tattgactca aatccaccaa ttatcttatc gtagatatat attataaatt taataagaaa
       61 gcgaggtgag aatcgtgaaa aaaacgatac taagtatttt ttcagtattt acaatattaa
      121 ttgggggcta ttttcttttg aatttagatt ctgttaattc aaacgctcaa ataaaagatc
      181 aaagagtagc tgaacataat aataaaaaaa tgagtttgac caaaaatatc tattctatgt
      241 cagatgaaga attgtataaa gaatttggtg atttatccgg ggaacagtta gaagaaagta
      301 ttccaggaat tagtgactca gataaaattg ttgtcttgtc tgctggtggg caattaatgg
      361 gagatggcaa atccgaatta gttagttata aacaaaatca cagacagttt attgatgtta
      421 atactgattc caacgctatt tcgattaagg atctaaaaga gcttttaaat agtaaaagaa
      481 atgaattaaa gcagataaaa gaaactaatt cagtggaatt agagtaaagg aagattcatt
      541 atgaaaaaga tttttggtgt ttctgttggg ctacttatga tgttaacatt tggaaatgca
      601 gtatatgctg ctgagaaaga agttatttca gatccagagc agtacgcctc acgatacttt
      661 gctgattttg aagaagaaga taaagtactt gcattgccag aaggtggttt tttaaaaggg
      721 gaagctaaaa ttgtatctat ggataatcca gatataattc aaggttcata taattcagaa
      781 actgatcctg cttcagtttc agtgacagaa gcaaaaaaaa ttgttgctga aaaattcaaa
      841 aatgatattg aaactactcc aattatgcct cgaggagcat ttccacccga tgttggtact
      901 agcggacacc ttcttgtact agcaggagga ggaagctata aatcacagcc atttagtggt
      961 gcgggatggc gttttggagg tatgggtgta aaaccagctg atggtacagg caacttttta
     1021 cgttggaaat cctacattga cgatggaaga gtaggaaatg taaatcaagc caacgcaact
     1081 aaatctggtt ctattcaagg aatggcattg tatgctcgtg aaggattcaa atactttgca
     1141 ccaaacgggc ctggttggac aatgaatata ggtatttatt acacttataa tcctgttgca
     1201 ggtacgtatt atgttgttca aaatatttaa taaaacagca caaagagatt tctttgtgct
     1261 gttttattat ttttagttgt tgacaaacac taaaatataa aatataatca acacataaac
     1321 gaacgtatgt tcggcaagaa agcaggggat ttttatggat gagatggttt taagtactca
     1381 gaaatggcta aacaagaaat acagtaatgt tacagggttt gataaggttc cagaaaatgg
     1441 gcgaacaggg tggcctacaa tctatggact tattgaagga ctacaagtag aacttggtat
     1501 tactaatcta gtagctaatt ttggacctac cactgaaaaa atgtacgata atcaggttac
     1561 tcctaaatgg ggcaaaaatc tacctaaaaa tattgtattt cttattcaag gtgccttttg
     1621 gtgtaagggg attaatccag gtggcttcga tggggtatat actccatatt tagatactgc
     1681 tgtaaaagag ttgcagactg atgcagggtt taatggaagc gctgtaactg gagttttgga
     1741 tgcgaaatgg gccaaagcgt tatttgatat gagtgctttt gttcttgttc ccggcggaga
     1801 tagtaaaatt agaaaaatgc agcaatggtt aaatgtaaat tatttagaat acacgggaat
     1861 aatgccttgt gatggtattt atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca
     1921 agctgaacta ggatattctc ccagtgaagc aacagggtct tatggtaatg ggacaactag
     1981 taaaacttat cctgtgtctg aaggtaactc gggaaactat gtaagaataa ttcaatatgg
     2041 attatatgtt aatggttatt ttgaagacgg tacattcgat ggtattttca ctaaatatat
     2101 gggtttagaa gtactggcat tccgaaaatt tatggtattg cctgaatata ctgaaatagc
     2161 agatgttgtt gtaataaaag gactattgtc tagcgctgga gatattagaa gatctgcaga
     2221 tggcgcagat acttctacgc aattaactag atctcaaatc caaacattgg ttgataatga
     2281 tataaaaata gttggtagat atcttactgg tactgttgga attggaaaag atgaacgaaa
     2341 taagtatcta actacagagg aattgaataa tattttttct aaaaatttat ctgtgtttcc
     2401 tatctatcaa gatggaggtg cagcattagc ttattttact tatgatcgtg gcttatcaga
     2461 tggtaagaag gctatagatg cagcaaaaaa tctaaatata ccattaacaa cagttattta
     2521 ttttgctgtt gatctagaca tgttagggga agaaattctt gcatatgctg gagaatattt
     2581 taagggggta tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt acggaacaag
     2641 aaatgtttgt tctcaaataa tcaataacgg gtatgcttcg tttgcatttg tttcagatat
     2701 gtcgactggt tatagtggga atttaggttt tcctatgcct agacaatggt cttttgatca
     2761 atttgttgaa tttaccatag gaagtggcaa cggagctgtt ggtatcgatt taatcgctac
     2821 atcgggtaga gatagcggat ttaatgagtt atcaaatgat actaataatg ataattacat
     2881 tgccaaatac aatcagaagg ttataaatca gatggtacgt gcgtatcaat atctatcaca
     2941 agctggtctt gataatcctt ggaaccctca tttgacattt tatagatatg taaattattc
     3001 tggtttgtca tgggacatca tttcttctcc tgtaacagag catgatagaa aaatttatga
     3061 tgaatatagg aataaattaa ctaatagcga gggcctgtat aattatttta tagatcctaa
     3121 tactggttca ataataggtt tgcctcactt aattgtgacg ttacaatcac agatgtttct
     3181 agccgacaca atcaatgata gtgtttctga ctttgcaggt tggttaggag atttaatgac
     3241 ttgctggggt gaagttaaga aattaggaat acaaatggag caaggtgtat ttgcattagt
     3301 aggtacggct gcaggtggaa catttagtct agaagattta taccaagatg ttgacgcggt
     3361 aaatgtttat accttagcaa aaagaaattc tgattcaaca gctttatcgg ctttaaaaac
     3421 gtactattgt gataagggtg ctattgatag atttaatcaa tttgttaaga acagatttgg
     3481 ttcttcagat aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg aaacagttga
     3541 tatcttaggt gtagggataa tttcattttt cattgataaa atagttaata attcacttgg
     3601 gacagatttt acttatcttg attttattgg agatgagggg aaagttgcaa atggattttc
     3661 tgaaaaaatt attcaatttc aagaaaatag aagtaaactt aattaagaat cgtttactat
     3721 ttaaacagtg tccttttcct tt
//
LOCUS       DAJSVD010000182         3702 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSVD010000182 DAJSVD010000000
VERSION     DAJSVD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.186"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            154..576
                     /locus_tag="ON498_002821"
     CDS             154..576
                     /locus_tag="ON498_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941027.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            586..789
                     /locus_tag="ON498_002822"
     CDS             586..789
                     /locus_tag="ON498_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941028.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1000..1584
                     /locus_tag="ON498_002823"
     CDS             1000..1584
                     /locus_tag="ON498_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941029.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1955..3280
                     /locus_tag="ON498_002824"
     CDS             1955..3280
                     /locus_tag="ON498_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3941030.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3273..3623
                     /locus_tag="ON498_002825"
     CDS             3273..3623
                     /locus_tag="ON498_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941031.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1325 a          537 c          717 g         1123 t
ORIGIN      
        1 caatattttt tgtaaataaa tgtcaatcaa gaaaatttaa acgattatga cattaaaaat
       61 atattttatg aattttctag tatataactt gatttatctg ttaagattta ttaagaaaac
      121 gttttcttag ttttgatttc gaggtgatag acaatgaaaa ataagtttat tttatcttta
      181 ggtattttaa ctattggaat tagtgtaact ggaactacaa ttcctacatt tgctgatagt
      241 acaaatgaac caactactgt agtagataat aaccaagaat taactccttt agataggaac
      301 ggaatagcca ttggtaaccc aactaacgac ttaactgtta ctgaaaatta tttaatagtt
      361 tctccattac ttgctcgagc agcttctaaa acgatacgag tgacaggaac gatgattttt
      421 gataaagttg gttggccttc tagacctcct caatcacgga attggaaaga aaaaagaaat
      481 ggttattggt actatggaaa attgtattta caaagttata aatcagctgg aaacaattgg
      541 attgcgactt attcaggaac cttaactaga aagtagggat tttttatgcc agaatttgtg
      601 aacacaccaa atttttatca agatagcgat attctaagta aaattaattt accgaaagaa
      661 gaatcagctt tacaagttca gcgaacttta caggagacac aaaaaattca aaaatcaatt
      721 tctaatctag aagattctga agaaaaaaga aaaaaaagag accaaaaaac atatcaaaaa
      781 gaaaaataat gattttaaaa aaagagtgtc tagtcgacat tctttttttc taattgaaag
      841 tgttcataaa atcacccatt tctggacact ttcttttttt gctttatttt tcaaaaaaac
      901 aattcatttc atgttcataa acccataaca atatctatgt tcagttaaac tatatggata
      961 aagttatgtt acaataaaat taatgaagga gtgatttttt tgaaaatagg atacgcccgg
     1021 gtatctactg gtttacaaaa tttggattta caagaagaca gcctaaataa atttggttgt
     1081 gaaaaaattt ttactgatca tatgagtggg gcaaaaagta atcgaccagg attagaaatg
     1141 gccattgatt ttgttcgctc tggcgataca cttgtcgttt ggcgcttaga tcgtttaggt
     1201 agaaatatgg aagatttgat ttcaatcgtc aatcgactga atgagcgtgg tgtgagtttt
     1261 catagtattc aagaaaatat tacgatggac aaatcaagtt ctacaggaca gctcatgttt
     1321 catttgtttg cggcatttgc agaatttgag cgtaatttga tcctggaacg atccgcagca
     1381 ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc gtcctgaaaa attgtctgaa
     1441 caagatttgg aattactgaa gactttagtg gatagcggta cacctattaa gactattgcg
     1501 gaacggtgga acgtctcaag aaccaccatt tatcgttatt tggcaaaaat ggaggagaaa
     1561 aatggaaaga caaattctca ttagtttttg tcatttaatg ctgaaaattt ttgccattta
     1621 tctagaaaag tgacaatagt atgagaattt tttgtaagtg tctactgata acttattatc
     1681 aaatgttgta aggattttat tcatttgtta atattaaaga acggaactcc aaaaaagtcc
     1741 gtactctaat ttgcagtctc tcactctttt agagtgagag tttttattag attttcaaat
     1801 taattagtaa agtgatagaa taaattgatc atacaaatca taagaagaag cagggtgata
     1861 gcatacctat gcttcttttt ttatgtattt agctatttct tctttacaac atcgaacata
     1921 tgtttgtata atgtggaaga atggagtgtt atttatgatg gattattcaa aagagccagt
     1981 aaatgattac tttttaatcg atatgaaaag cttttacgca agtgtcgaat gtatagaaag
     2041 aaatttagat ccattaacaa cagaacttgt tgttatgagt cgatctgata atactggttc
     2101 aggattgata ttagcttctt ctcctgaagc aaaaaagcgg tatggtatta caaatgtgag
     2161 tagaccacgt gacttaccac aaccatttcc taaaacacta cacgttgttc caccacgtat
     2221 gaacctatat atcaagcgaa atatgcaggt aaataatatt ttcagaagat atgtggctga
     2281 tgaagatcta ctgatttact cgatcgatga atcgatttta aaagtgacaa aatcactgaa
     2341 tctttttacg actgaagaaa cacgaagtca gcgaaggaag aagctcgctc aaatgatcca
     2401 agaacgtatt aaagaagagt taggtttgat tgctacggta ggcgtcggcg ataatcccct
     2461 tttggctaaa ttagccttag acaatgaagc aaagcataat gaaggattta ttgcggaatg
     2521 gacgtacgaa aacgttcctg agaaagtatg gaatattccc gaaatgacgg acttttgggg
     2581 aattggttct cgaatgaaaa aaaggctcaa ccaaatggga attttgagca ttagagacct
     2641 tgctaactgg aatccttata cgattaagaa tagattaggt gtgattggat tacagctgta
     2701 cttccatgct aacggaatcg atcgaacaga tatagcaatc ccgccagaac cgacaaagga
     2761 aaaatcttat ggtaattcac aagtgttacc acgagattat actagaagaa atgaaatcga
     2821 gctggtggta aaagaaatgg cggaacaagt agccattcgt atcaggcagc ataattgtaa
     2881 aacaggatgt gttcatttaa acattggtac gtcgatctta gaaacgagac caggattttc
     2941 acaccagatg aaaataccta tcacagataa tacaaaggaa ttgcagaatt attgtctttt
     3001 cttgtttgat aagtattatg aaggacaaga agtaaggcac gttggaataa cctatagcaa
     3061 gcttgtgtac accgattcgc ttcagctgga tttatttagt gatccacaaa agcaaattaa
     3121 cgaagaaaat ttagataaaa ttattgacaa gattcggcaa aaatatggtt ttacgtcgat
     3181 tgtacatgct tcttcaatgt tagaaagcgc tagaagcatt actagaagca cacttgttgg
     3241 tggtcatgcg ggcggaaatg ggggaattaa aaatgattga ttacaatgat cgtggacgtt
     3301 taaagtgggc agggttttat ttatctgatc atactgaaga aattgatgcc gacaatgaac
     3361 aacgaaccag tcaaaattta gcaaaagaac aaatgacgac tgaagagatt gcggaagtat
     3421 tgaacgctgc agtcataaaa aatagatcag tatctattca aaaagaagaa cgaaacgctg
     3481 aaggatacta tccagaagat attgttggca agatcttagg tagtgatgag ctggggatct
     3541 acgttggaaa agacaaaatt cactacgacg aaatacgtca tatcgaattt taccaggata
     3601 ataaatggag tcgattagaa tgaacggttc tgttgcaaag ttttaaataa agaataaaat
     3661 cccttacggt atctatgatt taagctagga ttcccaataa ta
//
LOCUS       DAJSVD010000183         3664 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSVD010000183 DAJSVD010000000
VERSION     DAJSVD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.187"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..277
                     /locus_tag="ON498_002826"
     CDS             <1..277
                     /locus_tag="ON498_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HCR3941032.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ON498_002827"
     CDS             420..641
                     /locus_tag="ON498_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941033.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ON498_002828"
     CDS             657..1499
                     /locus_tag="ON498_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941034.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ON498_002829"
     CDS             1562..3364
                     /locus_tag="ON498_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR3941035.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ON498_002830"
     CDS             complement(3423..>3664)
                     /locus_tag="ON498_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3941036.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAJSVD010000184         3628 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJSVD010000184 DAJSVD010000000
VERSION     DAJSVD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.188"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(452..751)
                     /locus_tag="ON498_002831"
     CDS             complement(452..751)
                     /locus_tag="ON498_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941037.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(856..1059)
                     /locus_tag="ON498_002832"
     CDS             complement(856..1059)
                     /locus_tag="ON498_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3941038.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1074..1820)
                     /locus_tag="ON498_002833"
                     /pseudo
     CDS             complement(1074..1820)
                     /locus_tag="ON498_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
     gene            1898..2206
                     /locus_tag="ON498_002834"
     CDS             1898..2206
                     /locus_tag="ON498_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941039.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2177..2434)
                     /locus_tag="ON498_002835"
     CDS             complement(2177..2434)
                     /locus_tag="ON498_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR3941040.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(2732..2872)
                     /locus_tag="ON498_002836"
     CDS             complement(2732..2872)
                     /locus_tag="ON498_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941041.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            3166..3486
                     /locus_tag="ON498_002837"
     CDS             3166..3486
                     /locus_tag="ON498_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941042.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3504..>3628
                     /locus_tag="ON498_002838"
     CDS             3504..>3628
                     /locus_tag="ON498_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR3941043.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
BASE COUNT         1067 a          649 c          614 g         1298 t
ORIGIN      
        1 aattgaaaaa tggatagtta ttatcgcaaa gattttctca tcgatatcaa ttatcattcc
       61 aatgacattg acgttttcta aagagcaatt taacgtagct actacattcg tatcttttat
      121 agctctattt tcgtttgctg ttcctaaaac ttttcaaagc aaatctagca acgcaaaggt
      181 taatgtagaa aacactgata gaaaaaatga tgaaagttat taaaaaaaat aattctttca
      241 tttattgtca aacctttctt atagacatta gactttttta gtcagtccct cccgactggc
      301 ttttttgttt tgtactcagc ttcatccagc cctataaaaa tccaaaccat gtacacaatc
      361 gtacctatca acgcttgtct gcttccccag aggcctaaag cgtagatgat tagcggtgcg
      421 ctgaatacta gtgctctgtt gaatttgccc attagcttgc ctccttcaaa acatctgaag
      481 gcgataaact ttttacacga tatttgtttg cgtctttcca ctttaaaaac caaataaaag
      541 tatggtaatg aataaatgtg gttgagtgac ctggacgaag tatcccttct gcaaattcag
      601 gaattgtttc catttccttg cagtattcaa ctaaagtagt ttttgacatg ccgtggaact
      661 tctccaagat taaactttgt cggtaccagt cgtctggttt tactgccttc tcagcagctt
      721 ctatcaactc tgaaagtgtt ggttttttca cttattaccc ctcctaaaaa ttatcgttcc
      781 atactcgatg tttaacattt cttgaagctc tgattttgta agaatgattt tatcttccaa
      841 cgactttacc tccctctaca attcgtacat agtgataatc gaatctataa ttctgtttgc
      901 ttctgcagaa gtctttttac cgtttaaaat taaagataag tagcttttgc taattccaaa
      961 tctttcagca agcatggtgt aagttaagaa ctttgaactt tcgacatatt ctttgatttt
     1021 ttctctatct cgttgagtga tttctgcaat gtcagtcata ctaaaactcc tttctaacca
     1081 atttcctcta aatccatttg agggtaatag ccctctgcga ttaataagtt gtaaataaac
     1141 actcgccctt tctgtgtcta tttggtattc attacaactt tagtgccgcc atcggctttc
     1201 ggtatctcac ttgtgtgcga ttttgtgtat ccttggttca tatgttttcg gtaaagtatc
     1261 cattgtccgc taactttatg ctgaacacct aactcgttta gcaatttgtt tagagcaatt
     1321 gccgacattc cgtaatcagc tgcaatttga gaagtagcta ccgtatctgt cgatgaaaga
     1381 atcgtatcta agtagctaat ctttggttcg tactcggcaa tttgttgttc gagtaattgg
     1441 ttcttttctt ctaaatccgc tgctaaacgc aatgcttgtg caaaactttg aggtacgttt
     1501 gaatagctac ctgtttttct aattgttgga aggacttcac tcgttaccca acgtttaaat
     1561 tttttggcag agggaagttt tgattttaag attaaactgt aaaggcctga ctcgttaatc
     1621 aaaacaactg ctgttccatt gacggtgaac gaatcgttct gcgtcttatc ctccaagtcc
     1681 acatgatctc gaatagcttt ttgcggattc gaatatccta gaacgtctgc tacatctttt
     1741 cctacaaaat atggttcatc atttactaaa atagttcgaa cttcgttttg ctcgaaatta
     1801 aaaatttgtg gtgtattcat atttctcatt cctttctttg gtataatttc cttatcagtg
     1861 tgacaggctg aaataattgt taaggaggtg aaaatttatg aaaccagaat ttgtaattac
     1921 tatgcttgat ggagaaaaaa tcaaaatatt tgccgatact ttggtcgttg gatacgatga
     1981 tgcgtcaaat atcgaagaaa aagatggaat gttctattct tctcaaattt acgtgaatac
     2041 cttgcaaggt gatattgggg tatctactct tgcaacatca agaccgcttg taggtcttat
     2101 cggctttttg tcaaaagcaa cattcttttc tctcggcgat gatatagatt cggatgaaac
     2161 tgtcatctat aaaacatcag ctgttaaaag tatcggtttc aaatagtaat tcttgctcgt
     2221 tgatagcgga agccaacttt tcttccaaca catcggcgag ctttcttttt gttggattca
     2281 tttcaaagtt gatagtcttg agaagcattt ttgcatcctt aactttttgt ccttgcaatt
     2341 ccttgatgat tttatcggct tggctatcaa ttccatgtgt tagatcatct atcatttttt
     2401 gtaccttgat tgtttggctc atttccttgt tcattgttta gcctccttta ataaatgatt
     2461 ttttgtattt tttaatttcc ataaattcct caaattgctc tttggtatta ttaccacgac
     2521 catacatatt gtgaaattct ttatggcaac tgacacaaag tgtaacgccg tttgaaatcg
     2581 ctgcggattt agttgttgca tggctaaatc acgaatcaca agtatctact aaaggcgaat
     2641 caatttcgcc aaaagaaatt gttcaagctt atttagatat tcattatgct gttatctatg
     2701 gtcagcttcc agaagaccgt aaaaacgatg actaaacgtg agctaaaatc tctgctatgg
     2761 ctgcaaccat ggcagagtct tctttatcta ccgcgtgttc catcgccact tttgcttgtt
     2821 tcagtatgtc taatttcaaa tcttcgattt ttgccgagac tgttttttcc atattcttct
     2881 ctcctttctt ttatattcgt aaacaaattt aacaactttt taaaaaactg cgttgactaa
     2941 attaacatac agtgttaaaa tcagaacata gttaaataag gcatataaac attgatttta
     3001 aaagctttct tggcggttgg catttattaa tcaatagtgt tttttgttgt ctttttagtt
     3061 gttaaacttg tttacaagaa tgatattaac accttttgtt aaaatagtca acgaatttta
     3121 acaaataaag ttaaatattt tttcttgaac gaaaggaata ttgatatgat aacgtttgag
     3181 agaataaaag agttagcgaa aaaacaggga aaatcattaa ataaagttga agaagaccta
     3241 ggctatggta aaaacgtctt atatagactt aagaactcaa acccctctac agaacgtttg
     3301 caagaaattg ctgactattt cgatgtctct gtagactatc ttctggggcg cgaagaaaaa
     3361 gaaaccccta aacatgtgga tttatcagaa aacgatactg ttttttcttt tgatggaaaa
     3421 gaaatatcta aggagacaat gcgtaaagcg attgcaattg ctaaagcttt agaggaaaat
     3481 gaatagttgg agtgatgggt tgtatgtatt tgaaattgaa agaaatgttg agcgaataca
     3541 atttgaaagt agtttacatg gaaatgaaag agcctggctt ctattatccg aagcctagaa
     3601 tcattttttt gaatgaaaat ctatatgg
//
LOCUS       DAJSVD010000185         3582 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSVD010000185 DAJSVD010000000
VERSION     DAJSVD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.189"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..473
                     /locus_tag="ON498_002839"
     CDS             <1..473
                     /locus_tag="ON498_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3941044.1"
                     /translation="ILTGTDYPTGGIGNHSVISAHSGIPNKKYFTDLELLKLGDKFFI
                     DISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDNSHRLLVRGVSIPY
                     QKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVAKRRGMKNEE"
     gene            463..1572
                     /locus_tag="ON498_002840"
     CDS             463..1572
                     /locus_tag="ON498_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3941045.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            1911..2711
                     /locus_tag="ON498_002841"
     CDS             1911..2711
                     /locus_tag="ON498_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941046.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            2799..>3582
                     /locus_tag="ON498_002842"
     CDS             2799..>3582
                     /locus_tag="ON498_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941047.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLL"
BASE COUNT         1371 a          473 c          716 g         1022 t
ORIGIN      
        1 gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct gttatatcag
       61 ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc aaacttggtg
      121 ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat cggataaaaa
      181 caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat tatgtaacac
      241 ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga ggggttagca
      301 taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta aaggtaaagg
      361 cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata gctgtgatag
      421 tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa taaaatacta
      481 ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa tcagctctat
      541 catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga gaaaaaacaa
      601 actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa cagcggtaac
      661 caaggattag acaatactca gaaaacagat aaaggagtag acaatactca ggaaacagac
      721 ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac aggagatgtt
      781 ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc ggttggttat
      841 gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc acaggagcat
      901 ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg taaaggtcat
      961 caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct ttatgttgat
     1021 caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc agtttatacg
     1081 gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac tgatattcct
     1141 gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta cgatcgttta
     1201 ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt aacagttggc
     1261 aataccgcat acgctgatgc cattgatagt gaatccgttt cagataagcc agaaactgtt
     1321 acatcttcta atgatttatt tgaggatgtt gatcgtgcca acaaggaatt agatggaaat
     1381 aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac gtttgctgaa
     1441 aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct tttagtcatt
     1501 tggactgtat taggagcatt agcaagtcta aaggttctta ttcttttaat caaagagttt
     1561 agaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg attgatacta
     1621 ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa aataaaaact
     1681 gatcttaatt ccaaagaaaa agtcaagaaa aaaatacaac aaattttttt atcagaatat
     1741 ttatcagaat attattagaa aagataaact caaaagctta atttaatagg gttattggtt
     1801 gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta gaaaatctta
     1861 tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta atggtggaaa
     1921 tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt gatggtgcag
     1981 aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa acactgaaaa
     2041 aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat gctacatggg
     2101 atgtagtgtg tacgatatat tctccttggg taacgattgc tgattttgtt ttagataaaa
     2161 ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt aaaacgttct
     2221 atggttatct aatgggtcta ataggcaatg gaatagattt gaaagttacg ccaaaacaaa
     2281 cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca gatgacagag
     2341 aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa atggtgcaag
     2401 aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaac gacgaagatt
     2461 tctttaatga aatgtttagt aatagtccat gggaagcagt gagtaaaaca aatttaggag
     2521 aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta cgatcatatt
     2581 cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg atgagctatt
     2641 taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa ctattaaaag
     2701 aagagctata gggattattt caacataaac acaagcagaa aactaccaaa caaaacgtac
     2761 catgcaaaaa gccggttaaa ccggcttttt gctctattat gagaggagat caaaccatga
     2821 gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga aatgtcgaaa
     2881 atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca gctgtaggag
     2941 aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa tttcctttta
     3001 tcgcatcgaa actaagtcaa tttgccgagg agaaatcgtg gaatgaatta gttagttttt
     3061 ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa atgaataatc
     3121 aatacaagaa aattgatgct ttcgtcaaat cacgtataga aggaacagct tttgagtttg
     3181 aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa agtatagcgg
     3241 atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa acagaaagag
     3301 atacagaggt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt gatcccaata
     3361 tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat tttgaggaag
     3421 ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag gaagtggacg
     3481 aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat tataaatatt
     3541 tgagtgatca aaactcggtt aattggagtg tacgattatt aa
//
LOCUS       DAJSVD010000186         3555 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSVD010000186 DAJSVD010000000
VERSION     DAJSVD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.190"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            304..1344
                     /locus_tag="ON498_002843"
     CDS             304..1344
                     /locus_tag="ON498_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3941048.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2168..2500
                     /locus_tag="ON498_002844"
     CDS             2168..2500
                     /locus_tag="ON498_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3941049.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3119..3460
                     /locus_tag="ON498_002845"
     CDS             3119..3460
                     /locus_tag="ON498_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3941050.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1313 a          472 c          662 g         1108 t
ORIGIN      
        1 atgcataagg tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt
       61 taattttaaa actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata
      121 tttatttaaa gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat
      181 tttaggtagt atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg
      241 ctattttcga aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct
      301 acaatgactg attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa
      361 tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt
      421 gcctatatgc ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa
      481 gaaggtaatg tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa
      541 aaacaaaaag cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag
      601 cagatgggat ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa
      661 ttaaaagtct cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa
      721 aacgttgata aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct
      781 gaatcccttg gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca
      841 gagggtatga aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat
      901 atagacactg aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt
      961 atgacaaacg acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca
     1021 ttttcttcaa gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa
     1081 aaagctgaaa tagaaagtga tatttcgata gtttttgaag aactcgatag ttattatgtc
     1141 aatgcagaac aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa
     1201 aaagtcgaaa atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa
     1261 ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact
     1321 attacgaaag actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt
     1381 aacattaaaa ttaaaaattt tttattattc taattatatt acgaatatta caaaataata
     1441 gctattttaa tagaatttat aagttaaaat attatgataa aaatcacaaa cgtagccttt
     1501 tacgtcataa gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt
     1561 gcatactcct tttttttgag ttataataat acgcgaagag agataatttc attatttaaa
     1621 caagctaaaa atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt
     1681 ttttgtataa atttaattgt ctgctatgct tgtgatgaat tgattttcgt tttgcacttt
     1741 tggatgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
     1801 ggctggtaac cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
     1861 cctttcagtg ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat
     1921 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
     1981 aagcataatg ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta
     2041 gagggaacgc atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt
     2101 ctctattttt gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt
     2161 ttattttatg tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg
     2221 gtgtaccgct acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt
     2281 gcagtgtaag aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat
     2341 tgggaaacaa aagcaaataa ttttaaaaga tcctatcccc ttaaaagagc tatatccaac
     2401 aatcaaacat gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt
     2461 gcgttgtacc gcaaaaggga tggggcgttt gatcggctga attgatcatt ctcataactt
     2521 gtattttatc gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagag
     2581 tcactagaaa aataaggagt agacttgatc tatcaacaat tcccttcggg gagtgacggg
     2641 gtaggtttta acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg
     2701 atagtggtga aatgaatttg tgtaaataat aagcgcagag taaacaagag ggaatttaga
     2761 agttagacaa gttagaatag cgtctagatt aatcatgagc agctactaag ggatctagac
     2821 tttgacgcaa actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga
     2881 aattgtgtcg tgtgctgcag tacatgtaga tacttgcgag gacgttgatt attggatcaa
     2941 tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa cgagtcgaaa
     3001 ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag
     3061 tgaaaaaatt aatataaagc atagagaaaa tgaaatttac gtaatggagg aatgacaaat
     3121 gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt ctttccaacg
     3181 ttgggattac aaaggatttg tgacgtttag aaggggcgga aattgtaaag gattagatgt
     3241 tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc caattggatt
     3301 cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga
     3361 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
     3421 ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
     3481 acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt accttaggca
     3541 ttgagtaagg aattt
//
LOCUS       DAJSVD010000187         3486 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSVD010000187 DAJSVD010000000
VERSION     DAJSVD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.191"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            153..386
                     /locus_tag="ON498_002846"
     CDS             153..386
                     /locus_tag="ON498_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR3941051.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            383..790
                     /locus_tag="ON498_002847"
     CDS             383..790
                     /locus_tag="ON498_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3941052.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1927..2445)
                     /locus_tag="ON498_002848"
     CDS             complement(1927..2445)
                     /locus_tag="ON498_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR3941053.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1184 a          590 c          528 g         1184 t
ORIGIN      
        1 agaagggagc aatctaaatt ttttgaattt tcttaattaa ttatatagaa agaacaatct
       61 ttctatagag ttagacatga gagggactat gcttgattta aaatcaacaa gcgtatatac
      121 tgtatataca aagaaaaagg agggagaaga ctatggcaat taaagaaaga aaatttaggg
      181 aagtggggaa ctcagtagta ttaactattt caaaggagtt tcttaaagaa aatgggttaa
      241 atcccggaga tacggttttt ttagatgaag aaaaacttcg agcggcaatt acaaaaaaac
      301 aagccttatc tgatgaaatt gatttagcaa tatctcaagc attaactgaa tatgatacta
      361 cttttagaga gcttgtcgat aaatgagtat tcattaccta gaagccaaag atattataaa
      421 aatgaatgta atccaaataa aaaaatactc tcctaatgaa ccccttggtg taaaggatgc
      481 gaatgcttta gaaatgtgtg tagggcaact tcgagcctca gcttttggtg aagaggttta
      541 tccttcgatt tatgaaaaag cagctatcct acttatacaa ttgataaaga aacatccttt
      601 tcacaatgca aataaacgta ctgctttctt agctactttt gtctttttga aaatcaatgg
      661 acagctttta gctattgatc aaaaagatgc tgtaaattta gcggtatata ttgctacgta
      721 tgataaggat ttcgatcaac taaaatatga agtgatcaac actataaaga cacatacgca
      781 gcccatttaa ggagttactg tatgtgtctt tatagggtta ttagctgcta tagcgaaata
      841 attgaagtaa attacaaata gctataattc aaatccaact atatagaaaa cgtaaatttt
      901 ctatatagtt ggattaagat aacgactatt tattttatca tatttttact ataccctata
      961 cttttacttg gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag
     1021 ttagattaat aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata
     1081 aattgatggt atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt
     1141 gacgattgct actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat
     1201 gaaatgaaag accttttaca tcttgcagaa atcggtactg ggattaaact aagcaataaa
     1261 aactaatgtt cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg
     1321 acgatttcac cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta
     1381 tcagtaatag gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa
     1441 atctgtatcg tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat
     1501 aagcgttgaa tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat
     1561 taccggactt gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt
     1621 cggtactatc ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc
     1681 tcctaagtcc ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg
     1741 gtttatctgt tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct
     1801 aggggagtgc acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt
     1861 cgtatgttct tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac
     1921 gggaatttac aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg
     1981 agcaactgta tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc
     2041 gtctgcccat tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat
     2101 catgttaata tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg
     2161 tgcataggta tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc
     2221 agaaggcgtt ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt
     2281 cgtccaacca acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct
     2341 tttatccttc aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct
     2401 atttatttct ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata
     2461 aattcctaat tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc
     2521 tacaatgcat atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata
     2581 tcccaacgtc tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat
     2641 atttcttttg tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa atatcaaaat
     2701 catgttaact actatcaatt actaataaaa actaccactt taagcgttaa gaactgttaa
     2761 gtttagttaa gtgaggatag ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa
     2821 ctcaaaatgc tttatatagg attacagcat cgctctttct tcgattgcgc gttgggtaaa
     2881 aaatattatg aagtaaaaat tgacgatgac acgttcctta cggctcatca gatgaaacag
     2941 ctacaaaaac gaaatgatca actcgaagaa aatttaattc taaaaaagcg attgccatat
     3001 tcacgcctcg ctcgacgaac tattaaaggt caagatttac ctgaagatca tcctttttag
     3061 gaaattgaag ctttttatgt tgcacaatgc atttataata ctacaatgat gttttcacca
     3121 gatattcttc tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc
     3181 gaatttacta aaattatgaa taattatgtt aatcttccga aatttttttc caaaattaag
     3241 ggatcaagct ggtgttatag attgtttaag gttggcaaaa aaataacttc tttttaaaca
     3301 attatagaaa accgatactc tgttaagttg gttgacacgg tgtcgatttt tatgactttc
     3361 ttattctgaa acttcactaa cggacagtac ctaaaaagtc tatagtatgg acgtttacga
     3421 gtactatttt atcaaaaatg ctgctcattt caacttatat gttttagtga actaccccca
     3481 cttagc
//
LOCUS       DAJSVD010000188         3468 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSVD010000188 DAJSVD010000000
VERSION     DAJSVD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.192"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..2233)
                     /locus_tag="ON498_002849"
     CDS             complement(<1..2233)
                     /locus_tag="ON498_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941054.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2905..3173)
                     /locus_tag="ON498_002850"
                     /pseudo
     CDS             complement(2905..3173)
                     /locus_tag="ON498_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          975 a          766 c          549 g         1178 t
ORIGIN      
        1 aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct ccttgaagga
       61 cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca aggatgtttt
      121 caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca tgtaccgtcg
      181 catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta cctacaggaa
      241 gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc acttgtgaat
      301 cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca tcagttttaa
      361 ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc aatcgattgt
      421 cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac gcctctacat
      481 tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc gcactgaaac
      541 gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat agaaatgaca
      601 caaattccat gccttcttcc aaaacatctt caataacaag cgtattgctt gttccagaaa
      661 acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata acatttttgt
      721 ccacactttt tcttaaagag attgccggaa gattactttc agggattctc aagcggtagt
      781 ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa aacccatcct
      841 cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat tcaataaaat
      901 acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt tccttattaa
      961 aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt gcttcacctg
     1021 gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt ccagtggaac
     1081 tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg gtaaccacta
     1141 caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta ctgttggtca
     1201 cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt tctctagagc
     1261 catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt gaaaattccg
     1321 tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt ggctcagtac
     1381 cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac tgagcggctt
     1441 catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct tcttggtaaa
     1501 atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac tcaattaagc
     1561 caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga atatcgccag
     1621 cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa gtggggcgct
     1681 cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc tgagattcct
     1741 gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag ctttcttctg
     1801 gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca cttgtgcttt
     1861 cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg cttcttgcgg
     1921 tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg taatcattga
     1981 gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat gcatttagca
     2041 ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc tcctcttgat
     2101 caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct agaaccgtta
     2161 tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac agtttatgaa
     2221 atttgttcct cactattact cccccttata attttgacta aacaacaagg tacccgataa
     2281 ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta ttttcggttc
     2341 aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt gtcttctgtt
     2401 gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc ataattatga
     2461 tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta atagttattt
     2521 tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta aatcgttgat
     2581 accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac cttcatcaat
     2641 cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga atacttggga
     2701 ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc aactaaacgc
     2761 atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta ctaataaact
     2821 gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc tttttgctct
     2881 ttatttgggc gtagttttac tttcttaact tgtatcgtct tccatcaact ccttaatcat
     2941 cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag tgacaatctg
     3001 tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta ccatatattg
     3061 caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta tagaataact
     3121 accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc ttttgttgta
     3181 gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg tattgctatt
     3241 tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca ttgattcccc
     3301 tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata ttagtcatac
     3361 tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga atttttccaa
     3421 aaaataacat ttttataaaa caacgaaaaa taaatcacta ccagtcaa
//
LOCUS       DAJSVD010000189         3270 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSVD010000189 DAJSVD010000000
VERSION     DAJSVD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.193"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            229..504
                     /locus_tag="ON498_002851"
     CDS             229..504
                     /locus_tag="ON498_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941055.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            546..968
                     /locus_tag="ON498_002852"
     CDS             546..968
                     /locus_tag="ON498_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941056.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            988..2580
                     /locus_tag="ON498_002853"
     CDS             988..2580
                     /locus_tag="ON498_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941057.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2601..2936
                     /locus_tag="ON498_002854"
     CDS             2601..2936
                     /locus_tag="ON498_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941058.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            3082..>3270
                     /locus_tag="ON498_002855"
     CDS             3082..>3270
                     /locus_tag="ON498_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941059.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPF"
BASE COUNT         1303 a          480 c          660 g          827 t
ORIGIN      
        1 acccactttc aaaataaagt gggtagatag gctgtgccta tcacgtttct gaaaaaaata
       61 aatgacccca aaattttcaa tttatgtgta ggtttatggg tagcaaaaat atgaatgtgg
      121 gcagcaaaaa gttcatttgt gggtagtaaa attcaaatgt gtgtagcaat gtgtgtagct
      181 acacacattg ctacacacgc tataacatat agtgaaaagg agatatttat ggaagtaaaa
      241 ataagagata ttgatgaaaa aacgattcaa agaattgacc gaagatgtat tgaattaagt
      301 gaacgcaccg ggactaaatg gagccgaaat cgatacctaa aagttttgat tgaaaatgat
      361 tttgaccatg aacttatgga tcatagaaaa gatcaatttg atcggttgtt agaaaaattt
      421 gtggatattc aaacttataa tacgaaaatt ttagaagaat atattgcaac aaataatcaa
      481 ttaatcgcct tgttaattga ataataaaaa tagtattgtg aatttataaa aggaggaaat
      541 caattttggc aaaagcaaca agaaaacatg tatatcttta cgatatgcat atcgaagtac
      601 ttgatgaaat aatttctact catgaagata tatctaatta tagtgaagca gtcagacatt
      661 tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa tccaattcaa attaaattga
      721 atgcaatggg aaaagaaatt tctatattaa ccgaaatggt ttcagagttt gcggatcttc
      781 atttgaaaga tacaggaata ctaattggta caaattcaac tgtctatcaa gaagcaaaag
      841 agcgtgtgga agggaaaata aaagcgaatc aaacgaaaaa gtattctcct aagatcaaga
      901 aaccaataaa tgaatctgtt catgccagag tagacccgtt tgatccaaca aagtttttaa
      961 aaagataaaa agtagaaggg gaacatgatg aataaatcgc ctgcagtcat tctcacttgt
     1021 caatttacct tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa
     1081 aaagcgctgg aagaaaagga acgacgcacg ccggacgaag aacaggaact gcaacgaatc
     1141 aagaatgctt tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagaca
     1201 gataaagaat tcagtgaaac gtcgaatgaa gctcaatcgt tgatggaaca tggaatcaat
     1261 tttgacaagt tagaggaaca agattttacc aaatatcttt cttatatgac ccgtcactat
     1321 gcgttagaac aaaagaaaga actgacgcaa agggaacaaa aagaatacca acggttgaac
     1381 caagcaatga aaaaatatca aacagagcca cataaaaaag cttcaggaaa tcttcctgga
     1441 gttttttcta tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caaacaaatc
     1501 ttccaaagag ggcaacaaaa aggctcaatc atttatcaag atgttgtctc tcacgacaat
     1561 tcgtatcttg aaaaaatggg cttgtacgat cccaaaacag ataccttaga tgaagaagga
     1621 ttaaaagcag ctggaagaaa aatgatggag acattattcg agaaagaaca attgaatgaa
     1681 acaggttact ggatggcaac gattcaccgt aacactaagc atatccatat tcattttgca
     1741 gttgttgaaa aagaaaatac gcgtaaacct tacacggagg ttgaaaatgg tgtgagttat
     1801 atcgtaccga aaggtaagag aagacaagca acattagacg caatgaagac caattatgta
     1861 catgagttag aacgtttttc ttatgaaaag gatcgaatga ttggtatgga gaaacgaaat
     1921 ttattgacgc ggaaaagtga tttgcgtaac accttaacaa aagtggtaaa agagccagcg
     1981 cgttacgatc gacacgcaat gaacttatta aatgaagtat ataaaaccct acctgacaaa
     2041 cgttcggaat ggcattatgg ggatgaaaaa cgaaccaaac tttcaccata cacaagagag
     2101 aaactcgatg agttaaccaa atatgtgtta acaaaagaac ctgaatatca agaatatata
     2161 cagatttcaa aatcattgaa agaagaagca aaaagtcttt atggggaaag caagagagaa
     2221 tcaaaggatg cagaaaaaaa tgctctcttt gatttgaaga agcgcacagg aaatgccatt
     2281 ctaacagaac tgaagaaaca ggatagtcaa ataaaacctt taatagaaag tatggatcag
     2341 atgcaatcta tttacaatga acgatccttt cgaacgctta aacagttacc aacgaatgaa
     2401 tacaaatata gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc
     2461 acgcaacgga gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac
     2521 gcaagtaaag cctacgaaga gcaacaacga agaattcaaa tggaacaaga aagacagtaa
     2581 agacagtagg aggaatagca atgaaacaat atttagatca atggaaagtt atagaaggat
     2641 cattaagaga agaacgaatc gaacaacttc ccgattgttt agaaaaagag catttgtttc
     2701 agataagaga aatgttgaga aacgaacagt ttgatccaaa tcaattttta gtcgttgagt
     2761 actcagctac tggtgtgtac tgttgtaatc atgtgaaggg tgaaaaatat tttatcattc
     2821 aagaatatga gggcaagtta gcgccttatt atactacttg ggaaatgaat gaagagggga
     2881 tcaataactt cccgtgtaaa agcattgaag agtctatttc actcacagaa tgttagttgt
     2941 taagtgaata aaagaagtaa aagagtcctg aacgtaattg tccaggactc tttttttcaa
     3001 tgaattgttt tccgccaatt caatgagtgg tgcgatttgc gactacatta tatcaaaaga
     3061 aagagaaggg aggaagaaga aatggcttat tcaacgattg gagttgttgt tgaaaaaagc
     3121 agagacaatc tcgtttttgt gacagaaatt caaacgggca gagcttttgt cgtcacagac
     3181 aaagcggcaa aagcgtatca aagtggcgat attttaacgt taaatctgac tacgaagacg
     3241 ttcgtcgatg cggcagaaga ttatccattt
//
LOCUS       DAJSVD010000190         3264 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSVD010000190 DAJSVD010000000
VERSION     DAJSVD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.194"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..622)
                     /locus_tag="ON498_002856"
     CDS             complement(<1..622)
                     /locus_tag="ON498_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3941060.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKP"
     gene            complement(633..830)
                     /locus_tag="ON498_002857"
     CDS             complement(633..830)
                     /locus_tag="ON498_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3941061.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(846..1088)
                     /locus_tag="ON498_002858"
     CDS             complement(846..1088)
                     /locus_tag="ON498_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCR3941062.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(1152..>3264)
                     /locus_tag="ON498_002859"
     CDS             complement(1152..>3264)
                     /locus_tag="ON498_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HCR3941063.1"
                     /translation="SSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQMDT
                     SGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQIL
                     TTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQITD
                     LTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFSQG
                     SGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDPSK
                     IGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDANN
                     SLSGKLRLRHAKLEEGSTATPYQPNLLDAPYYLSKVALGENLVTNAKFPIITPNNPIS
                     GFDISEELIIGETYTVSLKGTKPANKEFHLYYGDANYQQSQSQYQATLLPVEGLANVW
                     SATFTARNTSEMTNLLRVALWQKPNSATYDTVQIDWLKIEKGKTRTPNISEYKYRGIG
                     MRDSNNPYDYVWDIEPEYVEENLATESKVTEIIGEANKYTDNSIEAVNINVTNIADDL
                     AKQINVNENAARNYTDTKKIEAVNESKKYTDEVFRKEIVNLTVKNGNLGTARLYRQGN
                     CVTIYFFDLNGRNSGGNDSVILTVPEGYRPPISFEQLVGSTDRSAFNNAQLGFGADGN
                     IYWRRNTSYASSYTFTVTYII"
BASE COUNT          889 a          701 c          529 g         1145 t
ORIGIN      
        1 caggcttgtt ttcagaagtt gttggatttt catcggtaaa tccatgagct aaatcatatg
       61 ctaatttttc tttacttacg cccatttcag aaagataacc gtaaggatct gtatgatcgc
      121 cccagatgtt ttgtgttacc cataaatgtg atttgattcc tggttggtta taaggcgtgt
      181 ccaatgtaag cggaataccg tatttcttag cggaatctct cgccaattca acgtatgctt
      241 tatagttctt ttcaaaagtt gctttattat gtgtgtgttg taactcaatc tgcacaggac
      301 tgttggcatt agcatacgaa ccagcaccgt actgtacata accaggttgt ccaacttggt
      361 aaacaattcc gccatcaccc acaatgtaag cagtgtaagc actagtccat gaacgttgca
      421 tatactgcgc ttcattgcgt cctgttgctg tttcattagc cgtttcatgc agtaaaatgt
      481 actgattatt tgctacttgt gagctacctt catttgcgcc caaattaaat tcattgttga
      541 tagtataggc aaacccatta attggcaata aaaaaagagc cgttaatagg ctcatcgcag
      601 taatagtaat tttctttttc atttgtttcc tcctattttt tcaaattata agccgacaca
      661 ccagtgataa cacctaaaaa tgttgctact gcattgatag tgagtactgt catatctgtt
      721 ccattccatc catacgcttt ccctaacgtc gctactaata cagatgcagc tggcaatact
      781 gttaaaactg tccatttaat gacttgataa tacttatctg gtaaaaccat ttcttctcac
      841 ctcctttaca atttagtcaa gaaatagcca atgattgtaa tgcctaaacc gatcatgtaa
      901 ccccacgacc atttattatt ggctttcatc tctttgatgt cttctgcatt attaagcgca
      961 atagaatatg cctgatccgc tctatctttt gcactttccg ctttttcgcg taatgattcg
     1021 taattatcca gtttcgtttc aatacgcact aagcgttcta ccacgtcttg tattgcttcg
     1081 tctttcaatc aaacagcctc ctttctggaa ataactagcg cactcatttg agcacactgg
     1141 ttatctattt cttatatgat atatgtcact gtgaatgtat atgaagaagc atatgatgtg
     1201 ttacgcctcc aataaatatt accatccgct ccaaacccaa gttgagcatt gttaaaagca
     1261 cttctgtcag tcgacccaac cagttgctca aaactaattg gtggccgata gccttctgga
     1321 acagttaaaa taaccgaatc attcccacca ctatttcttc cgtttaaatc aaaaaagtaa
     1381 atcgtaacac agtttccctg tctatataat cgtgctgtgc ctagatttcc attctttaca
     1441 gttaaattca ctatctcctt tctgaaaact tcatctgtat acttctttga ttcattgact
     1501 gcttctatct tcttagtatc cgtataattt ctagccgcat tttcgtttac gttaatttgc
     1561 ttagctaagt catctgcaat attcgtaaca tttatattca cagcttctat tgaattatct
     1621 gtgtatttat ttgcttcacc aatgatttct gttactttgc tctcggttgc taagttttct
     1681 tcgacatatt ctggctctat atcccataca taatcatatg ggttattaga atcacgcata
     1741 ccaatgccac gatatttata ctcactaata tttggtgttc tggttttgcc tttttcaatt
     1801 ttgagccagt caatttgaac agtatcatat gttgcagaat ttggtttttg ccataacgct
     1861 actctaagta aatttgtcat ttcactagta tttctggctg taaacgtggc actccacaca
     1921 tttgccaatc cttccacagg aagtaaagtt gcctgatact gcgactgcga ctgctggtaa
     1981 tttgcatctc cgtagtataa gtggaattct ttattagctg gcttagtccc ttttaaagat
     2041 accgtataag tttcacctat tattaattct tctgaaatat caaagccaga aataggattg
     2101 tttggtgtta taattgggaa cttagcattt gttaccaaat tctcacccaa agccacctta
     2161 ctcaaataat acggcgcatc gagtaaattt ggttgatatg gtgtggctgt tgagccttct
     2221 tcgagtttag cgtgtctcaa acgtagtttg ccggataggc tgttattagc gtctttattt
     2281 tgtagcatta taagccagct ttcagcgtta tcactcgcag cagtaatttt aacggtgcca
     2341 gttagttttt gccatacacc cttagtagca gttaatgtat tacgcgtata tagctctgat
     2401 acccagttag gcattttaat atatcttagg cctatcttgc tagggtctcc agtgaaatcg
     2461 ccttctaaca taatttctac actcacggta tatgttttgc ctgttaatag ggctggttga
     2521 cttttcggtt ttaatacctc taatttatgg tttggatcaa gcgtaatcac tacctcgtca
     2581 ccatcatcta caacagataa ggcaccacta ccttgcgaga agctatcagc gtttatatta
     2641 gccatcaaat tcggattccc cgaataatca tagcccccga aatcaatgct gttactgtac
     2701 atcacttgta agttacctaa cttagaaatt tcttctttca gagcatctaa cttgtcttgt
     2761 agcgttttag cttgaccagt taaatcagta atctgttgat ttaagctatc cactctacct
     2821 ttagtttcag ccataaaagc atcaaaagtt tcattatact ttcgaatcaa ctcttctaat
     2881 tgcgaaacat attcatcggc ttggccttgc gaaatgtcag acactcctag tgagaaaaaa
     2941 atgatatctt gcgtcgttaa aatttgatcg tcttttctat attctacata gcagtgttta
     3001 taataccctg cttcactcat aaatgtgcca tcaagagaaa atgtgacttc ttcactagtt
     3061 acactagtag cgacactatc tacgtaacgg ttagatggcg tagttccttt taaagtaaat
     3121 gttccgccgc tcgtatccat ctgcaagcca tttagaaacg gtttaaccgt caccgtaatc
     3181 cctttatcac cttgacgagc cataatagct ttggtgtagt ttaattcttt gctgaaatct
     3241 aaagccaaat tatataaact gcta
//
LOCUS       DAJSVD010000191         3251 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSVD010000191 DAJSVD010000000
VERSION     DAJSVD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.195"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..687
                     /locus_tag="ON498_002860"
     CDS             <1..687
                     /locus_tag="ON498_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3941064.1"
                     /translation="WKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENA
                     WTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAG
                     NIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPV
                     VNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDA
                     GAVGGTLYAT"
     gene            677..1417
                     /locus_tag="ON498_002861"
     CDS             677..1417
                     /locus_tag="ON498_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3941065.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1414..>3251
                     /locus_tag="ON498_002862"
     CDS             1414..>3251
                     /locus_tag="ON498_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3941066.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1220 a          401 c          632 g          998 t
ORIGIN      
        1 tggaaaaata ttaaagatac ggcatacaat acgtggattt ctataaaaaa taccatgatt
       61 aatacatgga ataacattaa ggactctttc tggaatattg ttactggaat tgttaattct
      121 gctgaaaatg catggacaaa tctaaaaaat ggtgtttcaa atgctattaa tcgggtaaaa
      181 gaaatcttta attcgttaag ggaagtcaat ttatttgaaa ttggcaagaa catcattgat
      241 ggacttataa atggtgtaaa agaaaagtgg aatgctttga aagatactat caaaggtatt
      301 gcaggtaata tcaaaaattg gattaaagga gctttagata ttcattctcc atcaagatgg
      361 atgcgtgata tggttggtaa aaatattgtt caaggtatta tcgttggtat tgataaagag
      421 caaagtaagt tagatcaaac catgacaaat ttagtaaaaa caccgtctgt tcaacctgta
      481 gtaaatggaa taagtcacct accaattaca caaacaaagc aaaatatatc tgctagtgaa
      541 acaaaagaaa ttcatttaca tttaaatgtt tatggagatt tacctgattc aatggttcgg
      601 aagattacca caaaaattaa aacagaattg acaagacaaa tgaaacgaga tgctggtgca
      661 gtaggaggaa cattatatgc aacttaagag aggacaattt tttattaatc aacattattc
      721 ttctgaattt aatgtgtata ttcaaaacag gcctgcttct gtttcagcta gtcgagtaat
      781 tgaattgaga gagcgagaag gaaatgattc tatcattatt gataaaggct attacagaaa
      841 tgtgactaga aagattgagt gttactataa agcaccttca attgatgtgg tgcaggagtg
      901 ggaagatcga atcactgaat ggttagatat gtcgtcttat agtgatttta ttctttacta
      961 tgatgaacaa tatatttatc aagctgtagt aatagaagga cctgaattta aaggaacaag
     1021 aaaaacagga aatattgtcc cttttgaatt tacagtgagc attcgaccat ttaaggaaaa
     1081 ttacaatggt agatttacta ttcgacaaac agaaactttt gaaatttata acccagagaa
     1141 gtattcttca aaaccgctta ttaaattgag tggttctgga gatgcttctt tttatattaa
     1201 taaggacaaa tatgatttga aatcgttgga cagagaatta tttatagatt ctaaattaga
     1261 agaggcttac cgaaaattag atggtaattt agaacatcag gatcaagtca ctttattttt
     1321 agattttcca tttttatatc ctggaaaaaa tgaaattaaa tggacgaaaa atattcattc
     1381 atttgaaata atgcctaggt ggtggagaaa agtatgaaac caagaatata tagtcctact
     1441 gaaacagatt tctcaacgaa tggcttagga attttaaagg attgtacaag atgtgaaata
     1501 tatgaagtag ctaatggaaa atatgaatta gagttggatt atcctttagg aactagattt
     1561 gatgaatatt ttgaaaatga ctatcaaata aaagcaaagc caaacgatca agaagagtat
     1621 catatctttt ttattgatga taaagatata gatacttttt taaatacagt aactatttat
     1681 gctcagagtc gtacaaatcg acttggaaga cgggtagtca ctcttgcgga agtagactct
     1741 aaaactggtc aagaagcgat atcaattatt gaaaccaaaa tggataaaaa atctgacata
     1801 cgactttatt ctgatattac ggccgtttct agcacaatct ttgaagcgag aaatgtttta
     1861 aattgtattg ctggtgaaca aggatcattg cttcaatatt ggggtggaga aattaaacgt
     1921 gaaccattta agctttcttt gttaaagcga agaggtcgca ataatgttgg aacgattcgg
     1981 tatggaaaag atatgtctgg tttaaaggtc aaattagatt ggacaggtat aaaaacaaga
     2041 cttattccat atgctgatcc tcaaagtgag gtaggtacga ctaatcgaat ttatggttcg
     2101 ccagtagata gtgcctatat taataactat cctgatgtgt atacagagca tgttcagttt
     2161 acagaagaac aaggagtaaa agacattaag agcttaaata aaatagctaa aaattacttt
     2221 aaaacgatca atcctggctg tgataagcct aaagtttcta ttacggttga atttgataag
     2281 ttgactgata gtgaagaagc gaaagaattt gcgaagatta gaaattatgg tctgtttgat
     2341 acatttaaaa tatatcataa aaaatataat atttatcttg aatcaaaggt tagtggacta
     2401 caatacgatt ctttatctga aaaaactttg aaattagaag caggagacat tcaagttgct
     2461 ttttatcagc aacaagctgt aactattcag gataaattaa aagattatgc aacgaataat
     2521 tatatgagtg attttaatga ttatgtttct tcaatgatta caggtcaagg cagtgcagga
     2581 ggatatgtaa gtttatggcc aaaagaaaaa ccttccaaca tctttattat ggataatcca
     2641 gatttagaaa aggcaagaga agtactaaga atgaataaaa atgggattgc tttttctaag
     2701 aaaggatggc gggggccttt tacttcagct tggacattag atagtatatt taatgccaat
     2761 tttatacaaa ctggattaat caaagcagat atctttcaaa attcttttaa caagactgga
     2821 gatgtattga aattagttaa tggactactc caaatttgga acaacaaaaa gaaaataatg
     2881 gagttaacta aaaaaggaat ggaattttgg aattctaagg gttcaattgg aacgattgga
     2941 acaactgatt ctgctggtaa tccttttcct ggggcttcta ctccaacccc tattgaagat
     3001 aattctttag ttattcgtac aaatggagac ggcaaatata ttttgatttc tcctaaagta
     3061 ggaaaaggat tagttttatt aggaaatggt aaagcaattt attttggaga cttagatgta
     3121 caaggaaaac ttacagttaa aggaaaagaa ataattcctg gacaaggtgg tggtccatcg
     3181 ggaggtggag aatctactgg tgggtatcct agtgaattaa aaacagatgc agaaaagaga
     3241 gcttggagaa t
//
LOCUS       DAJSVD010000192         3211 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSVD010000192 DAJSVD010000000
VERSION     DAJSVD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.196"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..821
                     /locus_tag="ON498_002863"
     CDS             <1..821
                     /locus_tag="ON498_002863"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3941067.1"
                     /translation="TMVDSNGKPILNKYGKPKKVVKMPIDNSVTLSIQRLRHLASLGN
                     ESNKQIVDRYFSNQPEPLTINEQMELLQTGINLHSIYMVDENENEIITAPAIARWVIN
                     KHTLEVTARINPEYLEWFSEVVNQYILFNLRDFVKLKSKYSMILFLELSTFWNTGMYY
                     KRINDLREILGVNPTRNNRDFINNVLKPAIAALKEYFPELSLTVEKNSSDSRKITHVR
                     LKFDKLTITDMQERLRSSEGASLVEYQQELENMEGEEKAVNDPTPEIDLSNVEW"
     gene            935..1303
                     /locus_tag="ON498_002864"
     CDS             935..1303
                     /locus_tag="ON498_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941068.1"
                     /translation="MENTNQTPNTAPTNQTSPAERKADLIRLYSRLEGMKLQHVPTAP
                     KAPDGFPKSAESLEFFECKVCGSTFQTILRESVASGCPKCEHDRLGILADHLPEPDKN
                     SCLNNRKKPVTVMAMGFLCI"
     gene            1477..1785
                     /locus_tag="ON498_002865"
     CDS             1477..1785
                     /locus_tag="ON498_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941069.1"
                     /translation="MKEMKMKQVIYPVIIGVLAIALIILGVNCYNLSKENNQLETKNN
                     KQEIQIAKQAYVISDYKTYFRENYVDTVKYRSHFTDKQILNVYREALRDGKISDEGAN
                     "
     gene            2048..2368
                     /locus_tag="ON498_002866"
     CDS             2048..2368
                     /locus_tag="ON498_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941070.1"
                     /translation="MERFLTKNRKGGGKLEKKEDLLNAFKNLDEKDQLKALLRLHDIT
                     YSDIAERLTVKKGEREGQKGVSLVTVQKAFQSKTNLESDRWKEIVKVATKLIEERQND
                     QNKA"
     gene            2499..2708
                     /locus_tag="ON498_002867"
     CDS             2499..2708
                     /locus_tag="ON498_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941071.1"
                     /translation="MIRKLNNIKLSLAILTLFFGIYNNSRMALFFLTLLIFMNLIIHN
                     FKELENTAPAPSQGNQESEGMPHAW"
     gene            2698..2970
                     /locus_tag="ON498_002868"
     CDS             2698..2970
                     /locus_tag="ON498_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941072.1"
                     /translation="MLGSFKKDKEVKTRGALTPSQQNKLGTKATFHPVRDQANQKELI
                     GVCVGFTSEKFFPIIECKIHGITTREVARKVVKWHTSQTKTNHKKR"
     gene            3018..>3211
                     /locus_tag="ON498_002869"
     CDS             3018..>3211
                     /locus_tag="ON498_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941073.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAF
                     FIVAVNSSGGDKKEAASKDTT"
BASE COUNT         1212 a          577 c          647 g          775 t
ORIGIN      
        1 taactatggt agatagtaac ggcaagccta tcttaaacaa atatggaaag ccgaaaaagg
       61 tagtaaaaat gccgatagat aactcggtta ctttgagtat tcagcgccta cggcacctag
      121 caagcttagg aaacgaaagc aacaaacaaa tagttgatcg ttacttttca aaccagccag
      181 aacctctgac aattaacgag caaatggaat tactccaaac ggggataaat ttacactcta
      241 tttatatggt agatgaaaat gaaaatgaga tcataaccgc gcccgctata gcaaggtggg
      301 taataaataa gcatacgcta gaagtaacag cgcggatcaa tccagaatat ttagagtggt
      361 tttcagaagt ggttaaccaa tacattttat ttaatctaag agactttgtg aagttaaaaa
      421 gtaagtactc catgatcctt ttcttagagc tttctacctt ttggaataca ggaatgtatt
      481 ataaaaggat taatgattta agagaaattt taggagtcaa tccaacgaga aataacagag
      541 attttataaa caacgttcta aagcctgcta tcgctgctct caaagagtac tttccagagc
      601 taagcctaac agtggaaaag aattctagcg actccagaaa gatcactcat gtaagattga
      661 agtttgataa gctaacaata accgatatgc aagaacgcct aagaagcagc gagggagctt
      721 ctttagtaga gtaccaacaa gagttggaaa atatggaagg agaggaaaaa gctgtcaacg
      781 atccaactcc agagatcgac ctttccaacg tggaatggta acacctgtca gcaatctaat
      841 ccaataaacc aaagtacttg taaggcgcga gggctccttt tagagccttc tagtcctata
      901 ccaaaccaag caaaataaag gagctataac caacatggaa aacactaacc aaacaccaaa
      961 tacagcacca actaaccaaa ctagcccagc cgaacgcaaa gccgatctta ttagattgta
     1021 ctcacgcctt gaaggtatga aactccaaca cgttccgacc gctccaaagg cgccagacgg
     1081 cttcccaaaa tcggcagaga gccttgagtt tttcgagtgt aaggtgtgcg gatcgacttt
     1141 tcaaacgatt ttaagggaat ccgttgcgag tggctgcccc aaatgtgaac atgatcgctt
     1201 aggaatttta gccgatcatt taccagaacc agacaaaaac agttgtttaa ataatcgaaa
     1261 aaaacctgta accgttatgg ctatggggtt tttgtgtatt tgagataatt tccaagggtt
     1321 atttatggat ttattttaaa aaatctatta aattatacta ccactggtag taatataaat
     1381 atatcgaaag ggtacgaggg ttaaagcgta atggcggaat gagtaccgtt ctcttttgaa
     1441 aataccaaac caaccaatta aaggagagaa agtaatatga aagaaatgaa aatgaagcaa
     1501 gtaatttatc ccgtgattat tggagtttta gctatcgctc ttattatcct tggtgtaaat
     1561 tgctataact taagcaagga aaataatcaa cttgagacca aaaacaacaa gcaagaaatc
     1621 caaatagcca agcaagctta cgttatcagc gattacaaga cttattttcg tgaaaactat
     1681 gtagacaccg tgaaatatcg ctcccacttc actgataaac aaattttaaa tgtttatcga
     1741 gaagctctta gagatggaaa aatcagcgat gagggagcta actagtggaa gagctcattt
     1801 tgctggctat gttattacag gaaaagaagc gtgaacgaga agcctaacag ctggaaagaa
     1861 tccgaatagg atatgaggaa gcagatcggg aaagagatca gaaaatagag gagctggaga
     1921 agcgtatcca agaactttcc aaaaactatg ataataacca agattgggaa caagagtggc
     1981 ggaaaatcgt tggtataaag gaaaatgaat acttagtgag aaagccgcca gaagaagagg
     2041 aagacccttg gagcgatttc taactaaaaa tcggaaggga ggtggtaagt tggagaaaaa
     2101 ggaagattta ttgaacgctt ttaagaacct agacgagaaa gatcaactta aagcgttatt
     2161 aaggcttcat gatataacat acagcgatat tgctgaaaga ttaaccgtta aaaaaggcga
     2221 gagggaagga caaaaaggtg ttagcctcgt gactgtacaa aaagctttcc aatcaaagac
     2281 caacttggag agtgatcgct ggaaagagat tgtaaaagtc gctaccaagc taattgaaga
     2341 gcgacaaaac gaccagaaca aagcataaaa aaagcccgtc catttaccgc tgaccaaagc
     2401 aataaaatga acgagccaca ccatttactt ttaggggtaa ccacacgccc taaaagctac
     2461 caaaccattt accaaataac cactaaggag atttagaaat gattagaaaa cttaataata
     2521 tcaagctttc tttggcaatt ttaacactat tttttggaat ttacaataat tccagaatgg
     2581 ctttgttttt tcttacgtta ttgatattca tgaacttaat tatccataat ttcaaggagt
     2641 tagaaaatac agctccagcg ccctctcaag gaaaccaaga aagtgagggc atgccacatg
     2701 cttggtagtt ttaaaaagga taaggaagta aaaacacgag gagcccttac accaagccag
     2761 caaaataagc ttggtacaaa agccactttc caccccgtcc gagatcaagc caaccaaaaa
     2821 gaactaattg gcgtttgtgt agggtttact tcagagaaat tttttccaat aatagagtgc
     2881 aaaatacacg ggatcacaac tagagaagtt gctagaaaag ttgtgaaatg gcacaccagc
     2941 caaaccaaaa ccaaccataa gaaacgttaa aaacgttcct aagagcgttt gaaaattaaa
     3001 ttaagggaga tttaagaatg aacaacaaaa acaccaaaaa taccaatact aaccacgaga
     3061 agagaagctt ctttgataag cttttcaaaa agaagaagaa tggaaaagta tctattttga
     3121 aaatgatcgg ctggatattt ttagcctttt tcatagttgc tgtgaacagt agcggcggtg
     3181 ataagaaaga ggctgcttca aaagatacca c
//
LOCUS       DAJSVD010000193         3165 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSVD010000193 DAJSVD010000000
VERSION     DAJSVD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.197"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..185
                     /locus_tag="ON498_002870"
     CDS             <1..185
                     /locus_tag="ON498_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3941074.1"
                     /translation="NLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILEL
                     SGKKLKKVNKMNLEVE"
     gene            185..1561
                     /locus_tag="ON498_002871"
     CDS             185..1561
                     /locus_tag="ON498_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3941075.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(1847..>3165)
                     /locus_tag="ON498_002872"
     CDS             complement(1847..>3165)
                     /locus_tag="ON498_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HCR3941076.1"
                     /translation="PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLD
                     STHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKER
                     KISKADPESGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQS
                     KRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFD
                     CYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLM
                     DEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAM
                     NLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQ
                     LRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
BASE COUNT         1097 a          486 c          575 g         1007 t
ORIGIN      
        1 gtaatctaga cagtgttact gctggagaaa taattaatat attaaagaca ttagctaaag
       61 atagaaataa atgtgtaata gttgtaacac atagtaagga agtagcagat tctgcggata
      121 tcattttaga actaagtggt aaaaagttga aaaaagtaaa taaaatgaat ttggaggttg
      181 aataatgata aaaaatgcat ttgcttatgt aactagaaaa agtttaaaat cattaattat
      241 catattagtt attttatcta tgtcaacttt aagtctcatt agtttatcta ttaaagatgc
      301 tacggacaga gcttcaaaag aaacatttgc taatataaca aatagttttt ctatggagat
      361 aaatagacaa gtaaatccag gaacacctag aggtggggga aatgtaaaag gtgaagatat
      421 taaaaagata tctcaaacag atagtataga ctcctatgta aaaagaataa acagtgttgc
      481 agatttagtt gattatgata ttattgaaac tcaagagact cttgcgaatc aatcacctga
      541 aagagcaaag aattttaaaa gaacagttat gttaactggg gttaacgact caacaaaaga
      601 aacaaaattt gtatcagaag catataaatt agtagaagga aagcatttag aaaatgaaga
      661 taaaaataaa atcttaatgc ataaagattt agctgaaaaa aataacctta aagttggaga
      721 taagataaaa ataaaatcta atttatttga tgctgataat gagaaaggtg cagatgaaac
      781 agtagaagta gaaattaaag gtctattcga tgggcataat agcggtgtag taagtgcagc
      841 acaagaacta tacgaaaata cattaatcac tgatattcat acagctgcta aagtttatgg
      901 taataccgaa gatacagctg tataccaaga tgcaacattc tttgtaaaag gtgataagaa
      961 tcttgatagt gtaataaaag atcttgggaa attagatata aattggagag aatataattt
     1021 gattaaaagt tcatcaaatt accctgcatt acaacaatct atatcaggta tttactcaat
     1081 ttcaaataaa ttattttttg gctcattaat atttgcagga gttgtagttt cattattatt
     1141 attcttatgg atgaatgcta gaaagaaaga aatagcagta ttactatcat taggtatatc
     1201 aaaattagaa atttttggtc aattcctaat tgagatggta tttatatcaa tccccgcatt
     1261 cgttggttcc tatttcttag ctcaatatac agctgacaag cttgggaata atatattgaa
     1321 taaggttact ggtgatatag ctaaacaaat agctaaacaa tctgcatcta gccaattagg
     1381 tggtggggca gaagctgaag gatttaacaa gacattatca agtttagata tcaatgtatt
     1441 acctaaattc atgatttatg tagttatatt tatgagttta gtacttcttg tatcattgat
     1501 tatttcttca ttcaatatat taagaaggaa tccaaaagaa ttattaattg acaataatta
     1561 aaattttcgg tgctttttag caccaaaaat gagagtgtaa tataaaccgt gtaagtaacc
     1621 atcaatccta ggcgggtggt tgcttcacgg tttttatttt tctaaaataa gatcaagagg
     1681 tgattacacg atggctagaa agaaaagaaa tcctgatgct gagaagttag ctgaatccat
     1741 tttgaatact tatcaacccg aatctgtaga ggatatgcaa gatgttttaa aagatgtgtt
     1801 tggtccctta tttgaaaaaa tgcttcaagg agaattgaat aaccatttag ggtatgacgc
     1861 ccattctaaa gagccaaaga aacatgacaa tcgacgaaac ggttatggca ataagacgct
     1921 taaaacgagc tttggtaaag taaacattga tgttcccaga gaccgggagg cttcttttga
     1981 acctgagttg attcctaata gaaaacgaga tgtctccgac atcgcagact gtagacaaaa
     2041 gctgttccaa actcgtaact cgagcttgga acagcttttt atctacttcg tttcgaattt
     2101 ttgaacaaaa aaataaaggc tccttgcctt tccatagcca ggtggctaat ttctttaaat
     2161 tcattgcagc aaacactagc atcgtatgcg tcgcgacttt ttccaagcct cgatacttgg
     2221 tccaacgcat cccatgcttt tcttttgcat ccgcaaatat tcgttcaatc gtttgtttcc
     2281 gttttttgta aattgattta ttcagatctg ttaggcgaag atgttccact tcatccatca
     2341 gatctcccca aacatgtctc gtgatgactc gttgatgatt tttacttatg gtacattggt
     2401 ttagcagagg acagttcaca cattcttttg gattggattt gtattctcgg tacccttctc
     2461 gagtaaccgt tgaaaaagcg agcatttgat cttgcggaca aatataacaa tcaaaatact
     2521 cgtcatagag aaactcattt tttgataata gctccttttt ccctttgggc cttgaataag
     2581 gcaaacaagg tcgaaggttc aaatgagtca aaaagtgaac aaacttaggt gtcttatacc
     2641 cagcatcagc gacaacacaa ttgatttcag gaaaaagtct cttcgattgt ttgacgagct
     2701 gaaaggcgac ttgactgtca tgaatatttc taggagtgat gattgtgcta agaataaatc
     2761 cattgtcgtc gcatgaagta tggacagaat aagcaaattg tttttctcgt tcgcctttga
     2821 cgtagtagcc gctttccgga tcagctttac ttatttttct ttctttcagc ttcacttcct
     2881 tttctaagtc caaagtaaaa gggcgttttc cagcctctat tcgttgacga ttgatctcat
     2941 cttctaattc atcctgatag gctttcgctt catcatgggt catttctttc gtaaacttat
     3001 gtttattggc gttagctttg atgtgggtag aatccagata caaattatcc tctgttacaa
     3061 aacctgcttt aactgcttgt tctaaaatat aggcaaaaat atcctcaaac accgtcgttt
     3121 ctctaaatcg gcgaacataa ttctttccaa aagttgagaa agggg
//
LOCUS       DAJSVD010000194         3041 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSVD010000194 DAJSVD010000000
VERSION     DAJSVD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.198"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            138..752
                     /locus_tag="ON498_002873"
     CDS             138..752
                     /locus_tag="ON498_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941077.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ON498_002874"
     CDS             complement(776..1126)
                     /locus_tag="ON498_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3941078.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ON498_002875"
     CDS             complement(1120..1383)
                     /locus_tag="ON498_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR3941079.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1724..1903)
                     /locus_tag="ON498_002876"
     CDS             complement(1724..1903)
                     /locus_tag="ON498_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941080.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(2284..2991)
                     /locus_tag="ON498_002877"
     CDS             complement(2284..2991)
                     /locus_tag="ON498_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941081.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
BASE COUNT          925 a          589 c          469 g         1058 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacttg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa atattctaaa tagtaggaac
     1681 aattaagtgc tatcactttt gtttcttcaa atgaaaagtg acgtcacttt tttagtgctg
     1741 gtatatcaaa tcctgctttt tcttgcgcga tcaatagatc taacgcaata cacataatct
     1801 tagattcact ttttccgtac ttctgcttta gttgctgcaa ttctttttgt ctcttttcat
     1861 ctaatgtcac gggtaaccgt ttatttccta ttgcaattgg cattagtact ctctcctttc
     1921 atcgaaataa ccctatgaaa aatatggctg caatcatctt gaatagtaga ctcacaaggg
     1981 ctaatacaaa tcgccaaaag atcaaacaaa ataccagtat tcctaagaat actacgaagt
     2041 ttaaatgtac agctgcagca tgacgcataa gtatctcgcc aacaaacagt acgaacaata
     2101 gaggaatgat tttattcata tggatctcct tctttctctt aggttatcgg gctttgcccg
     2161 cttatttcgc accacctaag cggcgggggc ggttggagcg attggtgcga aataagaacc
     2221 attcccttaa tttcaaggtt ttttgttctt atttcacaca tcctcacttt tcggtataaa
     2281 tggttattta ccttttttcc tttcaaatcg ttgatatggc attcgtttac ggtcaagttc
     2341 attttggatt tcttctgtat cgtcgtaagt gtaggtgtct aaaaaatcag ttaaagtcgt
     2401 gtaccagtcg tcgcctgggt tatctacttc ttctacatat tccctcaaaa atgcttcggg
     2461 tacagcgagt tcataatcca ttcccattaa cacgatagat tccgtcccac acgtcaaaaa
     2521 cctagtaaat acaaggtaat aatcgtctac cgtcgcattt ttgctgttta gctcttccat
     2581 ttcttcttcc attaattcca tgctctcgaa aattgaataa tattctgatt ctgagtaata
     2641 gtgtttccta caaatcgtgt agctgacaaa gttttcgacc agctcactcc tttctaggta
     2701 cacattcatg atgtattttt ctgtttctag tcgtagaagc tctgttcctt tttttaccgt
     2761 ttcaggacga cgtttataca cgtctaaatg attatggctg actgtgacct tcgtttgact
     2821 tgtttcgctt tccatataca actgttcttc cactctttga ataaagtact tcatttcttc
     2881 catacaacta aagtcttttt gaatccgtct gattacttca ttcaagtgtt ctttgcttgt
     2941 taaaaattgc gaaacatctt ctgttgttag ctgataaacc accttattca ttatctttct
     3001 cctccgattc aatcgtataa aatgcaccat ttttttcgat a
//
LOCUS       DAJSVD010000195         3018 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSVD010000195 DAJSVD010000000
VERSION     DAJSVD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.199"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            36..1700
                     /gene="alsS"
                     /locus_tag="ON498_002878"
     CDS             36..1700
                     /gene="alsS"
                     /locus_tag="ON498_002878"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR3941082.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1713..2423
                     /gene="budA"
                     /locus_tag="ON498_002879"
     CDS             1713..2423
                     /gene="budA"
                     /locus_tag="ON498_002879"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR3941083.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2553..>3018
                     /locus_tag="ON498_002880"
     CDS             2553..>3018
                     /locus_tag="ON498_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR3941084.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANF"
BASE COUNT         1006 a          503 c          688 g          821 t
ORIGIN      
        1 taattaaaaa taaagcataa aaaggacgat gttcaatgga cgaaaaaatg aaacaaggtt
       61 cgaaatctgt agtagaaagt ttgattaatc atcatgttga ttatgtattc ggtataccag
      121 gtgcaaaaat cgatggtgta tttaatgaat tagaggatca aggtcctgaa ttaatcgtga
      181 ctcgtcacga acaaaatgct gcatttatgg cacaagcaat cggacgtatt acaggagagc
      241 caggagtagt tatcgcaaca agtggtcctg gtgctagtaa cttagctaca ggtttggtaa
      301 cagcgacagc agaaggtgac ccagttttag caattgccgg acaagtaaaa cgaagcgatc
      361 tattgaagtt gacccatcaa agcatggata atgcagcact gttccagcca atcacaaaat
      421 acagtgcaga aatccaagat ccagaaacaa tttcagaagt gattgccaat gcttatcgta
      481 tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat cccgcaagat gtagtggatg
      541 cacctgtcca agggaatgtc atcaagccat tgagcgatcc taaattaggt tctgcttctg
      601 cagatgatat ccgttaccta gctgaacgca ttagagaagc aaaactaccg gtactattag
      661 taggtatgcg aggatcaagt gaaaaagaaa cgttagccat caggcaatta gttggaaaga
      721 cagcattgcc tgttgtagag acattccaag cagctggagt gatctcacgt gaattagaag
      781 cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc aggagatatg cttttgaaac
      841 gaagtgatct agttattgct attggctatg atccaatcga atatgaagca agaaactgga
      901 atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc gccagctgaa atcgatcctt
      961 ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc cactcttgat ttattgacag
     1021 gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga atatttagca tctcttcaag
     1081 cagaattaac ggagcgggac attgttcaat caaaaggaga agcaggtatc ttgcatcctt
     1141 tagaagttat taacacgctt cagtcaaaag taacagatga tatgactgtt accgtagatg
     1201 taggtagtca ttatatttgg atggcacgcc actttagaag ttatgaacct cgtcatttgt
     1261 tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc atgggcgatt tctgctgcat
     1321 tggtgcgtcc aaatacacaa atcgtatcag tttccggcga tgggggattt ttattctctg
     1381 cccaggattt agaaacggcg gtacgtaaaa aattgaatat cgttcatttg atttggaatg
     1441 atggacacta caatatggta gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg
     1501 tagactttgg tccagttgat tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc
     1561 gagctacaag tgtagaggaa ttagaaaaag ctttagaaga aggatttgcg acagaaggac
     1621 ccgtaattat tgatattccg attgattacc gtgataatga gaaattggga gaaacaatct
     1681 taccagatca attctattaa ggaagtgtcg acatgactga aaaaatctta taccagcacg
     1741 ggacattagg agctttgatg gctggcttga tggatggaac agaaacgatt gctcatatat
     1801 tagaaaaagg aactctggga ttgggaacat tgcatgggct tgatggtgaa gtcatctttt
     1861 tagatggcgt agcttatcaa ggaagatcag atggctcagt tgcccaatta gatggaagtg
     1921 aacttacccc ctatgcagca attacagatt ttacgccaga tactagtttt tctgtctcaa
     1981 aaactgcaga tggagaacaa ttgaaaaaag acatattagt aagagaagcc ggggaaaacc
     2041 tatttttggc agtgaagata actggattat tcaaaaatat gcacatccgt atcatgccga
     2101 agcaagaaaa accttacaga agactagcaa aaatttcaga gtctcaacca gaattccaac
     2161 aaagcaatgt ccaaggaaca ttagtcgggt tctttacgcc tgaattattc caaggagttg
     2221 ctgcagcggg atttcacttg cattttatcg atgatacaca tacatttggc ggacatgtaa
     2281 tggattttga agtggctgaa ggaaaagttg agattagccg catctcgtcc cttgtacagc
     2341 attttccagt tgatgatccg acttttgttc acgaaacgat tgattatgct gatttagcag
     2401 cagaaatcca acaagcagaa tagaaaaaga cagcgagatg cgttcaaggt gattaacctg
     2461 acgtatctcg tttttttgag gaggaaacct agcgtttaca aaaaaactga gctataatct
     2521 tctccataga tcaaaaagga gcggttttta tcatgtggac agaaaaacag aaaaaagatg
     2581 catacgaaat tttattatta cagcaacaag gattattaga agcagaagat aactggttag
     2641 caaatttagc aaattcatct gcattgttga atgagacttt gcctgagact gtctttgcag
     2701 gatattattt attcgatggt aatgaattga ttttaggacc gttccaaggg cgagtttctt
     2761 gcacacgtat caagatggga aaaggagttt gtggagaatc agctgaaaaa caagaaacat
     2821 tgatcgttga tgatgtcaaa acccatgcaa attacatttc ctgcgattca gccgcacaat
     2881 cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat cggtgtttta gatatcgatt
     2941 gcggagtaac gaaagggtat gatgaaatcg atcaagcatt tctagaaaaa tatgtagatc
     3001 ttttattagc aaatttta
//
LOCUS       DAJSVD010000196         2991 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSVD010000196 DAJSVD010000000
VERSION     DAJSVD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.201"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            153..2213
                     /locus_tag="ON498_002881"
     CDS             153..2213
                     /locus_tag="ON498_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR3941085.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2296..2865
                     /locus_tag="ON498_002882"
     CDS             2296..2865
                     /locus_tag="ON498_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3941086.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1080 a          537 c          570 g          804 t
ORIGIN      
        1 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
       61 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
      121 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
      181 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
      241 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
      301 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
      361 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
      421 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
      481 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
      541 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
      601 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
      661 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
      721 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
      781 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
      841 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
      901 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
      961 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
     1021 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
     1081 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
     1141 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
     1201 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
     1261 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
     1321 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
     1381 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
     1441 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
     1501 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
     1561 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
     1621 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
     1681 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
     1741 aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
     1801 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
     1861 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
     1921 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
     1981 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
     2041 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
     2101 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
     2161 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
     2221 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
     2281 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
     2341 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
     2401 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
     2461 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
     2521 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
     2581 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
     2641 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
     2701 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
     2761 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
     2821 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
     2881 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
     2941 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg c
//
LOCUS       DAJSVD010000197         2969 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSVD010000197 DAJSVD010000000
VERSION     DAJSVD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.202"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..330)
                     /locus_tag="ON498_002883"
     CDS             complement(<1..330)
                     /locus_tag="ON498_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HCR3941087.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMD
                     EKTNKKDWTSSDVIRNGKHYVKPHYHVLYIARNPVTIESVRKKIKRKLGNNSVAHLEI
                     IDHIKGSY"
     gene            complement(411..560)
                     /locus_tag="ON498_002884"
     CDS             complement(411..560)
                     /locus_tag="ON498_002884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941088.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKK
                     SEKGN"
     gene            complement(1047..1226)
                     /locus_tag="ON498_002885"
     CDS             complement(1047..1226)
                     /locus_tag="ON498_002885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941089.1"
                     /translation="MEKDIYKQLTRQIQKEKEKGLLDHLDELELKADEINKNRMSAKE
                     KGIKLKDSSLQSDKS"
     gene            complement(1350..1595)
                     /locus_tag="ON498_002886"
     CDS             complement(1350..1595)
                     /locus_tag="ON498_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941090.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLKYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1940..2224
                     /locus_tag="ON498_002887"
     CDS             1940..2224
                     /locus_tag="ON498_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941091.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2227..2643
                     /locus_tag="ON498_002888"
     CDS             2227..2643
                     /locus_tag="ON498_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941092.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2780..>2969)
                     /locus_tag="ON498_002889"
     CDS             complement(2780..>2969)
                     /locus_tag="ON498_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3941093.1"
                     /translation="QMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTF
                     SSLTSDLPKISLRNWLEE"
BASE COUNT          895 a          575 c          455 g         1044 t
ORIGIN      
        1 atacgagcct ttgatatggt caattatttc taagtgagca accgaattat ttcccaattt
       61 tctcttgatt ttctttctta cgctctcgat tgttacagga ttgcgagcaa tataaaggac
      121 atgataatgt ggctttacat aatgcttacc gtttctgata acatcactac tagtccaatc
      181 ctttttattt gttttttcgt ccatatcgtg taacggacta accgccattg atactcctaa
      241 gctctcaagc tgttctctcc aattctctgg aatagattcg ggatatagta aaaaaccaaa
      301 atgtctagct ttcgttatat tcttattcat gatatactta cctcaattga acactatcag
      361 ttaagcggaa acggtgcgcc aacacatatg tttctgctct ttttttgcgc tcaatttcct
      421 ttctctgatt ttttcaagtc ttctgttact agcaatttaa catatgtcga catactcaaa
      481 cttttgtctt ttgctttttc ctcaagtttt cgcttaattt cactcggtaa aattactacc
      541 agtctttctg attccgccat gcctttcagc ccctttagtt aattgttaat atcatggtaa
      601 acttttgaca actgtattgt caatattttt ttctattttt caaaacggcg gtcaaaacgg
      661 cggtcaaatg gcggtcgaaa aaaaaaggct ctatccattg gtacagtaag gataaagcac
      721 tttttggcac aaaaattggc actcggcaca ctatgggggg gtcgtagtag cccccccaga
      781 ttttccaaat tccaaatttt ttccaaaaat tttccagcgc tatcgctcgg caaatcaaga
      841 ccgtaccttt ttgaaaaaag gcaccccaaa aactttccac tcgccgttaa ggcgtcaaga
      901 acgttcccta tggtcacctt cttgtgtttc cagcttttgg gttttgttta tagccgtcaa
      961 ctccttaaag cctcaaagag acgctaagag ggcttattag cctctctaag cgtctctaat
     1021 tcgatttacc gttgaccgca cgttatctag cttttatcgc tttgcaacga cgaatcttta
     1081 agttttatac ctttttcttt tgctgacatt ctgtttttat tgatttcatc agcttttagt
     1141 tctaattcat ctaaatgatc tagtaagcct ttttcttttt ccttttgaat ttgtcgggtc
     1201 aattgtttat aaatatcttt ttccatcccc catcgcctcc ttaaaagcta ttattattcg
     1261 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     1321 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     1381 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     1441 aacgatcgtt ttctttcggg cgtcatattt ttatatttca acaatttttc gtctgttaaa
     1501 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     1561 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     1621 ctttgccgtc tatactctca aaaaaaatgt cgtttgaaaa actaaataaa tttttgatcg
     1681 tgcattttta aatttggtta tcgatcacac gtgcagcact cccccccgaa ttttaaagag
     1741 cataaggaaa cgtggtatca ctttcttacg aaaggtgcta gataggctgt gcctatcacg
     1801 tttctttgaa aaaaatttga tatcactttt tcgccatcgt ggatagctta tgtatagtat
     1861 tttggcaaaa aacagctata caaatatagt atttgtatgg tatacataaa ttatacataa
     1921 ttgcaaaagg agatttttta tggacatttt agttcggaat attgatccaa agtttgttgc
     1981 aacaatagac aaacgttgcc aagaactcac gaataaaaca ggagaaaaat ggagtcgaaa
     2041 taaatatcta aaagttttaa tcgaaagtga ttttgaccgt caacttacga cttacaaaaa
     2101 agaccaattc gatttattgc tcgataaatt tattgctatc caaacagaaa atactcaaat
     2161 attaagcgag tatgtctaca caaataatca aatcattgaa atgcttatcg gaggaatgat
     2221 ataaccatga gaaaaacttc tgtccaaaaa catgttactt tgtaccctag tcatgttgac
     2281 gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga agccattcgc
     2341 tttttatgta tgagttatga acccgatacc aacacgacca ctaccagcaa tcaggcaaaa
     2401 ctaaatgcta tgggaaaaga aatttccatc atgactgaaa tcatcgcaga atttgctgat
     2461 ttatatctaa aagatacagg cgttcttatt ggcacaaatt ctaaagtata tcaagaagtg
     2521 aaagaaaaaa tcgaagctaa actaaatcca tcaaatcgaa atcgaccaaa gaatcatcaa
     2581 aaagaaacca gtcaaacagt cacttctaga aatttcgacc caacaaattt tctaaaacga
     2641 taaaaaacag ataggagtta ttcctatctg ttttttattt agaagcccca tacaatatgc
     2701 tagaatgtac tagcaatctt agtcggttgt aagggacttg tctacttgct taatgtaagg
     2761 ggggcgagtt cttttttatt tattcttcta accagttacg cagagaaatc tttggcaaat
     2821 ctgacgttag gcttgaaaaa gtaaattcta atttggcgat tttattgccc ttttttgctt
     2881 ttatttttgt gactttaaga tcattgaagt atttttttag ttctcgtaat gcaggattta
     2941 atactttttg atcaatgtta cccatttga
//
LOCUS       DAJSVD010000198         2946 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJSVD010000198 DAJSVD010000000
VERSION     DAJSVD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.203"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..140
                     /locus_tag="ON498_002890"
     CDS             <1..140
                     /locus_tag="ON498_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3941094.1"
                     /translation="TVECDCGERAKCIKSYCLYQCPTCGKKYTLSYGDYILIDEKGKK
                     R"
     gene            140..391
                     /locus_tag="ON498_002891"
     CDS             140..391
                     /locus_tag="ON498_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941095.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            407..844
                     /locus_tag="ON498_002892"
     CDS             407..844
                     /locus_tag="ON498_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941096.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            837..1532
                     /locus_tag="ON498_002893"
     CDS             837..1532
                     /locus_tag="ON498_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941097.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            1913..2092
                     /locus_tag="ON498_002894"
                     /pseudo
     CDS             1913..2092
                     /locus_tag="ON498_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2154..2338
                     /locus_tag="ON498_002895"
                     /pseudo
     CDS             2154..2338
                     /locus_tag="ON498_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            2372..2803
                     /locus_tag="ON498_002896"
     CDS             2372..2803
                     /locus_tag="ON498_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3941098.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         1065 a          455 c          612 g          814 t
ORIGIN      
        1 atactgtcga atgcgattgt ggagaaagag cgaaatgtat caaaagctat tgtttgtatc
       61 aatgtccaac atgcgggaaa aaatatacct tgtcttatgg cgattatatt ttgattgatg
      121 agaaaggaaa gaaacgctga tgaaaacaca acacaaatta ttgactgtaa aagaagtcgt
      181 tcaaattttg gtggacaaag aagtcatttc tgaaaaagta gcagaagctt tacttattcc
      241 gcggtactgg acgatagaaa cccctttagt agaggatgca ccgactatgc tatttgaaga
      301 cctagtgaag attgcaggat atgaactgca ttatcaagaa aacatggaaa cttatgaaaa
      361 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaaatatgg ctcaaaatta
      421 ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg aggatatgac
      481 attcctatac aatgagacac gtgttccaaa aaaacattat aaggaaagat tgtcggtagc
      541 tgtggaagaa atgattgaat cgggtgtcga aatgaatctc attgcgacct actatcgaac
      601 gcttgaagaa ctgaaaaagc aaaatggaca atggtttttc caagccttac tatgcttgga
      661 agcaggcgtc aagccaaaca ctatcaaacc ttctgaatac caagctttag aactaacgta
      721 tgggaaattt gtcgaaacga aaaaagccaa gactgtttca agtgaatggt tggactactt
      781 agaaaatgtc aacaagtatg gcgcatgtta catcatgaaa aaggaggaca acgaaaatga
      841 ataaggtgat ttatcaacta acaacggaag atgtgtcaca ctttttaaca aacaaagaac
      901 atttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa gaaatgaagt
      961 actttattca aagagtcgaa gaacaattgt atttggaaag tgaaacgagt caaacgaagg
     1021 ttacagtcag caataattat ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag
     1081 gagcggagct tctacgacta gaaacagaaa aatatatcat gaatgtttac ctagaaagga
     1141 gtgaactggt cgaaaacttt gtcagctaca cgatttgcag gaaacactat tactcagaat
     1201 cagaatatta ttcaattttt gagagcatgg aattaatgga agagctaaac agcaaagatg
     1261 cgacggtaga cgattattac cttgtattta ctaaattttt gacgtgtggg acggaatcta
     1321 tcgtgttaat gggaatggat tatgaactcg ctgtacccga agtatttttg agggaatatg
     1381 tagaagaagt agataaccca ggcgacgact ggtacacgac tttaactgat tttttagata
     1441 cctacactta cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat
     1501 atcaacgatt tgaaaggaaa aaaggtcaat aaccatttat accgaaaagt gagaatgtgt
     1561 gaaataaaaa caaaaaacct tgaaattaag ggaatggttc ttatttcgca ccaatcgctc
     1621 caaccgcccc cgccgcttag gtggtgcgaa ataagcgagc aaagcccgaa aacctaagag
     1681 aaagaaggag atttatatga acaaaattat tcccttatta ttagtcctat ttgttggaga
     1741 aatacttatg cgacttagtg cagctgtaca tttaaacctc gcagtatttt tggtaatact
     1801 aatgttttgt ttgatctttt ggcgatttgt attagccctc gtaagtctac tattcaagat
     1861 gattgcagcc atatttttca taggattatt tcgatgaaag gagagagtac caatgccaat
     1921 tgcaatagga aataaacggt tacccgtgac gttggatgaa aagagacaaa aagaattgca
     1981 gtaaccaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt
     2041 attgattgcg caagaaaaag caggatttga cataccagca ctcaaaaagt gatcgcactt
     2101 tgcgcacgtg ttaatacgtg ttataattat tttgtaagga ggtatagtgc acaatgccac
     2161 ttacgggaaa agaaatgctt aagttattga aaaaaatggc tgggttgagc gaaggcaaga
     2221 gggatttcat catcatcttt ataaggatgg tgttagaatc actgttcctg ttcatgccaa
     2281 tcaagatctt ggccgaggac ttgagcgaaa aattttaaag gatgcgggat tgaaataatc
     2341 tcgcattcga ttaaagaaag gattgactga aatgttaagt gagcctttag caaaaaccta
     2401 tcctgctatt tttagcccag aagaaggtgg tgggtacttt attgaatttc ctgatgttca
     2461 aggtgcttac acaggaatta acgaagacga tatatcttat ggaattgcta tggcacaaga
     2521 ggtgttaggt atggttttag cagattatat tgagcatgaa gatctacttc cagaaccaac
     2581 accaatcaat aagatttctg ttgaagatga ttcttttact actttaattc gtgtagatgt
     2641 tgccaaatat ttgaaagaca cagaacttgt caaaaaaacc ttaacaattc ctcaatgggc
     2701 ggataagcta ggcaaaaggg ctggcataaa tttttcagtc cttctaacag aatcaattgc
     2761 agataaagca gataacattc ttcgttctgg aagaaataat tagaactcaa aagggtcaga
     2821 aacttaaaaa tctttggctt ctttttttac tacaaagatg gcttctttaa attatttgcc
     2881 tgattgattt aaaaaaattg cgcaaagact acgagctaat taagttgctc gtggtctttt
     2941 tttatg
//
LOCUS       DAJSVD010000199         2900 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSVD010000199 DAJSVD010000000
VERSION     DAJSVD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.204"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ON498_002897"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="ON498_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR3941099.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          838 a          567 c          441 g         1054 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa
//
LOCUS       DAJSVD010000200         2858 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSVD010000200 DAJSVD010000000
VERSION     DAJSVD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.206"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..272)
                     /locus_tag="ON498_002898"
     CDS             complement(<1..272)
                     /locus_tag="ON498_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3941100.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPV"
     gene            complement(402..986)
                     /locus_tag="ON498_002899"
     CDS             complement(402..986)
                     /locus_tag="ON498_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3941101.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1317..1550)
                     /locus_tag="ON498_002900"
     CDS             complement(1317..1550)
                     /locus_tag="ON498_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR3941102.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1565..2515)
                     /locus_tag="ON498_002901"
     CDS             complement(1565..2515)
                     /locus_tag="ON498_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3941103.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2512..>2858)
                     /locus_tag="ON498_002902"
     CDS             complement(2512..>2858)
                     /locus_tag="ON498_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3941104.1"
                     /translation="AKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAA
                     TAYLYANSLKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISP
                     KQKFLRKKERSL"
BASE COUNT          941 a          519 c          359 g         1039 t
ORIGIN      
        1 accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt aaaaaactct
       61 atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa
      121 taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac
      181 cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa
      241 gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca
      301 ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa
      361 aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag taattcaatc
      421 cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa
      481 tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt
      541 tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa cttatctaga
      601 caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc ttgatatttg
      661 gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa aatataatta
      721 ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc tatattttct
      781 aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc attaatatgc
      841 ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata aaaagcaccc
      901 gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa tccttctttt
      961 tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata atctctggtt
     1021 tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac aggacttgaa
     1081 gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta atgagaaaga
     1141 gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc taaatcattc
     1201 accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta ttaggataag
     1261 tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa agtcttttac
     1321 ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa ttgaaatcct
     1381 atatctaaat agtcctcata tctaagttta gccagcatct cacttacaga tgctggacta
     1441 acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt tgataattcc
     1501 tgaattgcta gtaaataaat tagtatagta gtcgttttaa tttgtaccat acctaccctc
     1561 caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc atcgcatagt
     1621 aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag
     1681 gaatattggt atcttttgat actgttttca ttggtctatc atccacactg ctttctacga
     1741 ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg
     1801 ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta tacgctttcg
     1861 agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc ggaattgaag
     1921 caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac ttatctagat
     1981 tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt tcaatatttt
     2041 tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc ttccctttct
     2101 cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa tagtctttgt
     2161 tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct ccagtttcca
     2221 agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca tcttcaggca
     2281 aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa tcaattttat
     2341 cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact acttttaatt
     2401 tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc ccgaatataa
     2461 aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc atcataacga
     2521 tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa ttacaaataa
     2581 gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat aaccaataaa
     2641 caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca tagttttcaa
     2701 gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa caattaatat
     2761 agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca tcaatagata
     2821 atgataaaaa ctgacattca tccctattgc ttttgcca
//
LOCUS       DAJSVD010000201         2791 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSVD010000201 DAJSVD010000000
VERSION     DAJSVD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.207"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1350
                     /locus_tag="ON498_002903"
     CDS             <1..1350
                     /locus_tag="ON498_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3941105.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2791
                     /locus_tag="ON498_002904"
     CDS             1484..>2791
                     /locus_tag="ON498_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3941106.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1092 a          383 c          542 g          774 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat t
//
LOCUS       DAJSVD010000202         2779 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSVD010000202 DAJSVD010000000
VERSION     DAJSVD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.208"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            140..1147
                     /locus_tag="ON498_002905"
     CDS             140..1147
                     /locus_tag="ON498_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3941107.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1159..2163
                     /gene="neuB"
                     /locus_tag="ON498_002906"
     CDS             1159..2163
                     /gene="neuB"
                     /locus_tag="ON498_002906"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR3941108.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2160..>2779
                     /gene="neuC"
                     /locus_tag="ON498_002907"
     CDS             2160..>2779
                     /gene="neuC"
                     /locus_tag="ON498_002907"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR3941109.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQV"
BASE COUNT         1023 a          349 c          541 g          866 t
ORIGIN      
        1 tttgatactt catattttaa tcaatcaaat gttgattcgt tacgttttga catcattatt
       61 gataattagt ttagttatat attttttaaa gattaagaat cgaattatag atgactataa
      121 ttcataggag gagatataag tgtttaaagg aaaaacaata ttagtaactg gcggaactgg
      181 ttccattgga agtgaaattg ttcgtcagct tttaaaatat gaaccaaaag ctatcagaat
      241 ttatagtaga ggtgaagaaa aacaatttta tatgcaacaa gagctctcaa atcacactaa
      301 tttgagattt ttaattggtg atatcagaga ctctcaaagg attgattatg catgtcgtga
      361 tgtggatatt atttttcatg ctgccgcaat gaaacacgtt ccagcaagtg aatataatcc
      421 tatggaagct gtaaaaacaa atgtgatggg aactcaaaat gtaatagatg cagcgatcaa
      481 aaataatgta aaacattttg taggtatcag tacagataaa gtagtgagtc ctactaacac
      541 catgggtgcg acaaagttat tgagtgaaaa attaataata gctgccaatc agtataaagg
      601 aaaaacagaa acaatatttt cttgtgtaag gtttggaaat gtcatggggt caagtggatc
      661 tgttattcct ctttttagta aacaaataaa aaacaataaa gatattactt tgacagatgt
      721 tgatatgaca cgatttatga tgtcaatacc ggatgctgca agattagttc tgtctgctgc
      781 tatgaattca aaatttggag aaacatatgt cttaaaaatg ccggcaatca atattttaac
      841 tttagctcaa tcattaataa aaatatataa tgatacacat gatgaaaaat atagaggtga
      901 aataaaagtt gtaggtatca ggccaggaga aaaattatat gaagagctaa tgactacaga
      961 cgaaattgaa cgctcatatg aaaatgataa aatgtatatc attccaccac actatcatgc
     1021 tactgacgaa ctgtacaaag attatgaaaa aattaaaatc actgcatact cttcagatgc
     1081 aacagaatta atgagtggac aggaatttat tagaatcgca aaagagaata atgtcgtgac
     1141 ttggtaagga gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac
     1201 cataatggaa gtctagattt agctttagaa agtattgata aagctaaaga atgtggagct
     1261 aacgcggtta aatttcaaac atttaaaacg aataagctag tttccaaaaa ggctccaatg
     1321 gcaaaatatc agaaggataa tcttaaagag gacagttctc aatttgaaat gctaaaaaaa
     1381 ttagaattat ctcaatctga ttttattaga ataaaagatt attgtataca aaataaaatt
     1441 gattttttgt ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta
     1501 caaggattta aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat
     1561 atgtatgggt tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag
     1621 gctgtagatt gtttcataga tagtccagtt actattttac attgtacttc aaattatccg
     1681 gctagttctg aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata
     1741 cccgttgggt actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg
     1801 ggtgctaagg taattgagaa acattttacc ttggataagg aactacctgg tccagatcat
     1861 aaagcttctt tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa
     1921 atatttttag gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta
     1981 tcaagaaaaa gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa
     2041 tctcttacaa taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt
     2101 ggcaaaaaag ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa
     2161 tgaaaaaaat acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg
     2221 ctgagaattt actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat
     2281 cccataaata tggatttact aaagaattga tcttagctga cggatttaga attagtggag
     2341 aagttcaaag tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag
     2401 agataaatgg tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag
     2461 gggatagagg agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag
     2521 cacatattca cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa
     2581 cgaaaatagc atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa
     2641 tgggggaaga ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa
     2701 ataatagatt acctagtttt agagatataa aagaaaaata tcatctaaaa tttgaaaaga
     2761 aaaaatacgt tttgcaagt
//
LOCUS       DAJSVD010000203         2751 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSVD010000203 DAJSVD010000000
VERSION     DAJSVD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.209"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            149..1600
                     /locus_tag="ON498_002908"
     CDS             149..1600
                     /locus_tag="ON498_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3941110.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="ON498_002909"
     CDS             1949..2581
                     /locus_tag="ON498_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR3941111.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1044 a          391 c          529 g          787 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat a
//
LOCUS       DAJSVD010000204         2675 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSVD010000204 DAJSVD010000000
VERSION     DAJSVD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.210"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(838..2178)
                     /gene="glnA"
                     /locus_tag="ON498_002910"
     CDS             complement(838..2178)
                     /gene="glnA"
                     /locus_tag="ON498_002910"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR3941112.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2206..2598)
                     /locus_tag="ON498_002911"
     CDS             complement(2206..2598)
                     /locus_tag="ON498_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3941113.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          792 a          590 c          442 g          851 t
ORIGIN      
        1 aaaataaaac aaacattatg atagataata tcccacctaa caaagaacta ctttttccta
       61 cttgcagcaa cagtttatat agcttatcgt agaaacaatc tttttctttc acccttattt
      121 acctccttta cgtaaaaaac gtagtccaca gttctcggat ttttggcata tcttttctat
      181 taaataatat caaaaacaaa acaattccac taattttttc aaagatgacc tttttttcaa
      241 caaaagtgcc ttgtagggat tcattgaagg taacgaatca cataagtaaa aaataaggag
      301 atgcgacaaa agtcgtgcct ccttatttct aaataaacgg tgttctcctg ccaactcctc
      361 ggtcacaagt cacaatatcc atcaaacatg agaagctgtt tttgaaatta tttcttgtga
      421 ttcggagtta aatggtaggc tcacaacctc tggataaacg gtattcaaaa gccagtttct
      481 cggtattaag ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttaa
      541 tactcgaaac taaacgtctt tttcaaaacc tcttggtaaa cggtgttcaa aagcttgcat
      601 ctgcggtaat aagttcagcc aattcaaaaa caagaaaagc tgtttttgct tggctattct
      661 tattacttgt tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct
      721 gaccctcggt attagctcaa aaaacggcaa aatatgagga actatttttc cgttagtttc
      781 tctaatactc aggtcaaaac gctgatttca cagcctctaa ttcacggtat tcctgtttta
      841 ataaagttct agatattgtt ctctttccca ctcggaaaca gtttgtctga atgccgccca
      901 ttcgagacgt tttgcttcaa caaaatttga gaagatatgt tgtcctaatg cgtcaatcat
      961 tacattatcc ttacgcaatt ctttgatagc attatggata gttgaaggta agtcctggat
     1021 atgtgcagct tctctttctt cctcatccat tacataaatg ttacggtcaa cagctgctgg
     1081 cggtgtgatc tgattacgga taccgtctag tcctgcttgc aacaatactg ccattgccaa
     1141 gtatgggttt gcagatggat caactgaccg caattccaaa cgtgtggata aaccgcgaga
     1201 ttctggtaca cgtactaatg gcgaacgatt acggccactc catgcgacat aaacaggcgc
     1261 ttcataacct ggaacgagac gtttataaga attgactgtt ggattgcaaa cggctgtgta
     1321 ggcacgagca tgtttcagca aaccacctaa gaaatgataa gctgtttcac ttagttccaa
     1381 ttcaccgtct ttatcataga aaacatttcc ttcatcattg aacaaagaca tattgcaatg
     1441 cataccagaa ccattgatcc caaataacgg tttcggcatg aaagtcgcgt gaagtccatg
     1501 cttgcgtgca atcgttttga caacgagctt gaaggtttgg atattatcac aagcatctac
     1561 cacatctgca tatttaaagt caatttcatg ctgtcctggc gctacttcat gatgtgaagc
     1621 ttccacttca aagcctagac tttctaattc caacacgatg tcacggcggc aattttcacc
     1681 taaatctgtt ggtgcaaaat caaaataacc acctttatca ttcaaggtcg ttgtgatccc
     1741 accgtcttcg tctaatttaa ataagaagaa ttctggttca ggtcctaaat tgaaagaggt
     1801 aaaaccaagg tcgcgcatat ctttcaatgc tcgtttcaaa ttcccgcgag gatcgccggc
     1861 aaatggtgtc ccatccggat tatagatatc acaaattaaa cgagcaactt ttccatgagc
     1921 actttcccac gggaagatca tccaagtgga gagatctgga tacaaataca tatcgctttc
     1981 ttcaattcga acaaatcctt caatcgaaga accgtcaaac atcattttat tttctaatac
     2041 tttgtcgatc tggctcactg gaacttctac atttttgatc gtacctaaga tgtctgtgaa
     2101 catcaatcgt aaaaatcgta cgttttcttc ctcaatgatt cgtttgattt cttcacctgt
     2161 tatctgtttc tttaccatgg tatccctctt ccttttttaa tttattcagt caaagaattg
     2221 tctttgcaat ccttgcgact gataatgacc tgttttgctt aacccacctt gcgccaaaat
     2281 ctcatcacga aaaatttgtc gaacgtcatg atctgtcaac ggcttcttgt tttttgcttc
     2341 ttgttttttc gcctgtgcca tttcgtatac acgtttgatc ccagccatat tcaatccctc
     2401 tgaaagatag tctttaatct caagcaacat atcaatatca ttcagcgaat acatacgtcg
     2461 gtttccttcg cttcgttccg gatgaactaa atcttgttct tcataatagc gaatttgccg
     2521 agcagacaaa tcagtcagct tcataactgt accaattgga aatacggaca tagatcggcg
     2581 caattccttt tctcccatca gacgtcccct ttcgttttat aagttggcta aagtgtacca
     2641 attaaaaaaa atattgtcaa ctgcatatgt taggt
//
LOCUS       DAJSVD010000205         2641 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSVD010000205 DAJSVD010000000
VERSION     DAJSVD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.211"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            13..336
                     /locus_tag="ON498_002912"
     CDS             13..336
                     /locus_tag="ON498_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941114.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            500..754
                     /locus_tag="ON498_002913"
     CDS             500..754
                     /locus_tag="ON498_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941115.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            824..1069
                     /locus_tag="ON498_002914"
     CDS             824..1069
                     /locus_tag="ON498_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941116.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1145..1510
                     /locus_tag="ON498_002915"
     CDS             1145..1510
                     /locus_tag="ON498_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941117.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1571..2134)
                     /locus_tag="ON498_002916"
     CDS             complement(1571..2134)
                     /locus_tag="ON498_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941118.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          775 a          473 c          419 g          974 t
ORIGIN      
        1 tatttattct caatgttagg agtgattatt ttgccattta ttgcgatttt attcgattta
       61 gttgcggcag cagcttattt tctccaatta aattatcaaa cctcaggaat tctgtttatt
      121 ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg tcatgatgct cagctataaa
      181 ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta aatatgtttc cattcgctat
      241 gggatcatca ttctttcctt tttagtgaat gcgatcgtcc tctttttata tgtattgaat
      301 tacttagata tcaatccatt gattttttct cgatagataa gacacattca gcggaacagt
      361 tcctgcttat cagcagaaaa ctgttctgct ttttcatttt tcattgatct attcttcttt
      421 tattttccta gcatttcatt tgtgatgtat gcaataacat gttatctttt attatatgaa
      481 tcgttgagga gtccgttaca tgattacgtt ggtgagagtt ttgttttggg tgcctgcggt
      541 cgcactggtc gcaagtattg tttatcttat gaattggaac aaagaacggt tttatttagc
      601 tattttgact cttccggcta tctactttat gtggaaagtt tttaattaca actactttga
      661 acccgatagt gtttttatag aagaattatc aggactggtt ttgtctttat tgatcgtcat
      721 tctttatttg atacgtttaa ataaaaaaca ttgagtacta ttgattgcat gtgtagcttt
      781 ctatagtcat tataactatt ctactataat gaaaggtgat taaatgagta tcgaagaagc
      841 tattttactg gatactttac gttgtcgtct gaatttctta agaactggcc gatctttatc
      901 tcaagtagct gtttctagcg cgttaagtat tcctttattc aaatatcgta agtacgaaga
      961 aggagaagaa attccaagca tcattgaatt aatctatatc gctgactttt acgatatttc
     1021 cgttgattac ctaattggtc gctcagaaac agcaagcaaa ctgaagtgat tttttccagc
     1081 cttggtgctg gttttttcat ttaacgtcta aggttaacaa tctatatata aaggagacat
     1141 ttctatgcga ttatggcatg aaaaattgat ttcaaagctt cctcgtcaac agcttttagg
     1201 acaacatcgg gaatgctgtg cattaagagg aaatggctgg ggaaagaaac atgctactgt
     1261 ggattatgtt tttcactatt ctccttatag attattccaa tatcatcagc ttgtcatgga
     1321 tgaaatggaa aaacgtggtt acagcccagc accagagtgg aaaaatccta tgtatcgtgg
     1381 caaatcatgc gaagcatttt ttgatttacc tactgtagag cgcaacgtgc cgatttatcc
     1441 ggaacacgat gaagtttatc tagcagaatg tttagcaaat ttggagcaaa aaggaattta
     1501 tcttacctaa tataactaga aaagccttct atctcctctt acttcattaa aaagctttaa
     1561 ccatcttaga tcaagctaaa atgtcgtaag ttttagggtt atcttcatcc tcacgtgcaa
     1621 aactaaaagc gtactctagt tttggttcat cgccaacttc cctagacata aatgaacatc
     1681 tcctgacgtg gtttaaaagt accgcatctt cgacgtctgt atacgatgca cagcacagat
     1741 tgcttctttt gatctttctt ttgatttttc tgttgcttct tctacacctt cttcgattac
     1801 tacatcgatg cttacaattg cttctgccta atattcccat gcatcgtcga tttaagcctc
     1861 tgtagtcact gtgatcgttt ccacatttta actcctctgt cgtggcttca gttgactttg
     1921 tgatcagttc agttgctaag ccagagtcag aacaacctgc cattgctagc gttaatattg
     1981 atagtaaaaa tagttttttt atcataaggt tccccccctg aaaatagact aaaataataa
     2041 cgctttcttt ttttgttttc agacgaaaat taataatttc taaatattct ttaaaaaagc
     2101 ttataactat tatgcctttt ttaatatata acacagttat ttttaaaaat aagaatgcta
     2161 aatcactcta caataacggc gaaagcacaa aaaataaaaa aattattacg ttattaaaaa
     2221 aacagatgaa agatcaggtt ttattagatc tcattctttc atcacttgaa gggatttaat
     2281 gaactacttt ttcctctttg tcgtttttat atattcgtat tccttcaaac agtaggatag
     2341 cctctttcaa tgcttgatat ttgaactgtc cgttacttaa aaatttagat gtgccgccct
     2401 cttcctagaa catattcttt tttacttttt cattaattga cagaccaatc gaacgaaact
     2461 gtaaatttcg ataggataat gattggtcaa actcattcat tttcaagaca tctcgattca
     2521 tattcaagat aggaatcttt ttatcaacat cactttattc gtattttcct ctaccataaa
     2581 agcttcacgc tccacaaaat tgttttatac actactattc agcccattca acgaccaatg
     2641 a
//
LOCUS       DAJSVD010000206         2397 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSVD010000206 DAJSVD010000000
VERSION     DAJSVD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.212"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..619
                     /locus_tag="ON498_002917"
     CDS             <1..619
                     /locus_tag="ON498_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3941119.1"
                     /translation="RFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQS
                     EDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEW
                     ERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFY
                     PILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            632..>2397
                     /locus_tag="ON498_002918"
     CDS             632..>2397
                     /locus_tag="ON498_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR3941120.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT          890 a          285 c          476 g          746 t
ORIGIN      
        1 cagattttta ggtttagtta aaacattaga aaaaaatgat gtaacatcga ttaatcgatt
       61 aaagcactgg gtatctctaa caaaagaatt gtctaatcca tctttgaaga gggaaataga
      121 atattctcag tcagaggatt tattaaaagc tttaaaatgg tcggaagaag tcaatcaaca
      181 gattagtcaa gcaaaaggat tagataaacc ctttttaagt gctagagata ctataaaagc
      241 tctgaaaaaa tatgggaaga taattattgt aagctcagct aataaggaag ctgttcaaga
      301 agaatgggaa agacatgaat tattatcatt agttgatgaa ctgtgttgtc aagataaagg
      361 aaaaaaagaa gatattattc gaagtgtaat tgaaaatggt tgtgatttgg ataagatatt
      421 aatgattggt gattctcctg gtgatctaga agctgcaaat aaaaataaag ttttctttta
      481 tcctattttg gtcaataaag aaaaagaatc atgggaaaac ttaagaactg atgttttatt
      541 taaatgttta tcgggagatt atgtagatat tgaacaaaaa tatatagata ctttttggaa
      601 aaatcttact aataagtaga ggagaaagaa aatgactata aaagtagatt tacgaaaaaa
      661 accatataat ttagatgaag atgctatttc ttggattgaa gagacgattg attctatgac
      721 tttggaagaa aaaataggtc aattgtttgt taatatggga tctagtcgtg ataaaaatta
      781 cttgagtaat atggtcaatc agtaccatat tggagctgta cgatataatc caggaactgc
      841 tgatgaggta tatgatcaaa actatatctt acaaacagaa agtaaaattc ctctattaat
      901 tgctgctaat acggaagcag gtggtaacgg agcttgtaca gatggtactg aagttggtgt
      961 agaagtaaaa attggtgcaa caaatgatgc gaaatatgct tatgaattag ggagagtttc
     1021 tgggattgaa gcatcggcta ttggatgtaa ctggagtttt gctcctattg tagatattaa
     1081 tagaaattgg agaaatccta tcatttctag tcgatctttt ggacaaaacc ctgataaagt
     1141 tttagaactt agtttagctt atatgaaagg tattcaggag agtggaattt tgcctgcagc
     1201 taaacatttc cctggtgacg gtattgacga acgagatcag catctatctt ttagtattaa
     1261 tagtttaggt acggaagaat gggataattc ttttggaaaa gtatactcag gcttaataga
     1321 agctggatta ccttcaatta tggcaggtca tatccatatg ccagcttatt caaaacattt
     1381 taatcctgat ctgaaagatg aagatttact tcctgcaact ttatcaaaag aattaatcac
     1441 tgacttactt agaggaaaac tcggatttaa tggattagta gttactgatg ctagtcatat
     1501 gttaggaatg actggggcaa tgaaacgatc agaattattg cccacttcta ttgcagctgg
     1561 atgtgatcta tttctatttt ttaatgatcc agatgaagac tttaattata tgatggaagg
     1621 ctatcgtaaa ggaattatca ctgatgatcg gttattagat gccttaaaaa gaattttagg
     1681 gttaaaagct aaattgggat tacacaaaaa agcaaaagaa caaattttat tacctaagca
     1741 agaagctctg gaaaaaattg gtttaattga aaataaatca atttttaaag aggtggctga
     1801 ccaagcaatt acattagtta aagataaaca agatatttgg cctattgtac cagaaaaata
     1861 taaaaggatt ttactagtag atgtacatgg ggtaaaaggt gggtttggtg aagtagttgc
     1921 tggaaataga cctcaagcca ttgatttatt gaaacagatt ttgtcagcaa aaggatttga
     1981 agttgaaaaa tacatttcac ctgaagaaga attgaaagga ttatcgcaag aagaaaaaat
     2041 taaaaatata gctggaatat atgctgctaa gagaccaatt aaaaatttaa ctaaaaaata
     2101 tgacttgatc attaatatag ctaatgttaa tccaggcaca gttcaacgaa ttgtctggcc
     2161 agcaagtaaa ggaactccgg atataccgtt ttacattcat gaaattccaa caatttttgt
     2221 atctgtacag tgtccgtttc atttggctga tgtacctcaa gtaaagactt atattaatac
     2281 ttatgatgga aaacaagaaa ctttagaaat attggtggac aaactaatgg gtctttctga
     2341 atttaaggga gtaagtccaa tagatgctta ttgtggttta aaggatacat ttattta
//
LOCUS       DAJSVD010000207         2390 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSVD010000207 DAJSVD010000000
VERSION     DAJSVD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.213"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            197..2059
                     /locus_tag="ON498_002919"
     CDS             197..2059
                     /locus_tag="ON498_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3941121.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ON498_002920"
     CDS             2059..>2390
                     /locus_tag="ON498_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3941122.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAJSVD010000208         2377 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSVD010000208 DAJSVD010000000
VERSION     DAJSVD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.214"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(4..249)
                     /locus_tag="ON498_002921"
     CDS             complement(4..249)
                     /locus_tag="ON498_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941123.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(266..1117)
                     /locus_tag="ON498_002922"
     CDS             complement(266..1117)
                     /locus_tag="ON498_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3941124.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            1283..2287
                     /locus_tag="ON498_002923"
     CDS             1283..2287
                     /locus_tag="ON498_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR3941125.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT          758 a          402 c          478 g          739 t
ORIGIN      
        1 tttctatttt ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg
       61 ttcgtgccag ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat
      121 ttgatcatgt gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa
      181 gacaaaatcg gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat
      241 tggatccata aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc
      301 cgactacacc atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa
      361 ataattcagt attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt
      421 cgtgttgcag cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga
      481 tttgtttcat ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga
      541 ggatcatatc atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa
      601 ggaaaacttg attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt
      661 ggggtaagaa attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa
      721 atgctcgcca tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg
      781 gccagtggat caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa
      841 aagaagacat cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata
      901 tctcttctct aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt
      961 atccttgggc agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa
     1021 cagcgacatc cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac
     1081 catttgatag acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg
     1141 ttcaactata agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga
     1201 aatttttgtt taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac
     1261 taagacagaa gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat
     1321 tgacagagaa actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata
     1381 tattcgtgat tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg
     1441 acaactagca gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt
     1501 ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc
     1561 acctagtgca gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt
     1621 attgaaagtc aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat
     1681 cgacaaccgc ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga
     1741 agcattcgct gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt
     1801 ttctggcaaa aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc
     1861 tttagaagaa aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga
     1921 gacagaagaa tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga
     1981 aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc
     2041 aaaaatggct tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt
     2101 aaaagaagta caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa
     2161 cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt
     2221 gaataatccg gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg
     2281 tttataaaaa tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt
     2341 gtcaagagaa ggatcggaaa agacgaatat aacaaaa
//
LOCUS       DAJSVD010000209         2373 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSVD010000209 DAJSVD010000000
VERSION     DAJSVD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.215"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1327
                     /locus_tag="ON498_002924"
     CDS             <1..1327
                     /locus_tag="ON498_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941126.1"
                     /translation="AFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1314..>2373
                     /locus_tag="ON498_002925"
     CDS             1314..>2373
                     /locus_tag="ON498_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941127.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLI"
BASE COUNT          783 a          366 c          406 g          818 t
ORIGIN      
        1 tgctttttta tttagttgtt tatatacttt tgcaacttat cgaacagttg atatctatta
       61 tcgatccgca attgcagaag caatcgctct tacgattatc ccagctgttt tatattatgc
      121 ttatggattg atattcaata gagaaaaaaa agtcatcgct ttatctgtca gtataagtct
      181 acttgtgtat actcatgtct taactacact attgtgtagt gcattgattg cttgtatgat
      241 tatagttcgt ttgtttttaa gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa
      301 tttagtaaag gctgtttttt tgacttgtgc attaactagc tatttttggt atcctatgtt
      361 gcagcagatg aattatcaag aaatacacag tcccttcaaa acagatttgc agctagaagc
      421 actttctatt tcagatagta tcattcaagc gttaaataac gatattacaa catacactat
      481 ggggttatta ggattggttg cgttgatttt tcctttattc atactgaaag aaatggagaa
      541 aaaagaaaaa atcatctatg ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt
      601 tccatggttt ttgtttcaaa aaacaccgat aagcttactc cagtttcctt ggcgtatctt
      661 aggtatccaa atcttttttt cttcaatgat cataacgctt gtttttatga aaaagactat
      721 gaataaaaaa tattcatggt taatcattgg cgctactgtg gtgtttctac tcgtcacgtc
      781 tatagcagca aaagaaaatt acgcaaaagt tatccaatca aaacacggtc atattgttat
      841 caatgaagaa acactcccac gttatacaac atcagatatg ggaggattat acgattacgc
      901 cccaacagaa gcgataaaag aaaggcctca cttagaaaaa catgaagtaa aagtaggaga
      961 atcgtgggaa aaagggaagt ataaagctgc agataataac attacttaca cagtggcaag
     1021 caacaaaaag caaaaagtga cagtaccagt atatgcttat ttaggtacac aggtaaaagt
     1081 aaacggtaaa gtcgtaaatg actcttataa aaaacatggc ttagtgaatg tggatgtaaa
     1141 tgctggagaa aataagatag aaattacaac aaaatatact ccaactgctc tcatggcttt
     1201 atggatcagt attctgactt attgtttagt ggtgtattca agttttaaac gatttgcacc
     1261 taataagttg aaagattctc gatatgtgac taaaaaggag ataaacaaga agaatgaaaa
     1321 taaataaaaa agtagtaata ttgagtggat taattatagt tagtagtata tattctttca
     1381 tctttttcaa aaatacacag agtatttata ccactggaga tctttctttt cacttatctc
     1441 gaatcaaggg gttatcgact atttttacca gtccgattaa ttatgagaca ttcaattata
     1501 ctggatatgg tgtaaattat ttttatccct ttctgacttt ttttccagct gtaatgttat
     1561 attggatgac aaaaaatttg attgtaagct atattattta cgtttggtta ttgaacttgt
     1621 gtacaacact cgtagcttat cattatggcg agcggttttt aaaacaaaaa aaagcggcat
     1681 ttttatttag ttgtctctat attttctctg cttatcggac agtagacatc tactatcgtt
     1741 ccgcaattgc cgaagctatc gcaataacac ttgttatccc tgtattattt tatgcttatc
     1801 aaatcatctc tggaaaagaa gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt
     1861 tgctagtgta cagccatgta ctatcaacat taatgagtac agcattaatt atcattttta
     1921 tttttattcg tttggtttct aaaggtttca agaatgcaga ttttattgct atatttaaaa
     1981 aattatttag tgcggcagga atgacattgg ttctgacgag tgctttttgg tatccgatgt
     2041 ttgaacaaat gctctaccaa aaaatcaaca aacctagcgt gacaaattta tatgctcatg
     2101 catctaatgt gttcgatagt ctgactgaag ctatgaacaa cgacttaact acttactcaa
     2161 tgggactagt tggtctgtta tctttatgta ttcccctgat tttatttaaa aaattaacaa
     2221 acatagaaaa aaagatttat tatggtacgt gtttgacttg gctagctaca actagtttgg
     2281 tgccatggta tcttctgcaa aatacgccag ccaaattgtt acaatttcct tggcggatct
     2341 taagcttgca aataatattc tcttctctga tat
//
LOCUS       DAJSVD010000210         2351 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSVD010000210 DAJSVD010000000
VERSION     DAJSVD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.216"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..544
                     /locus_tag="ON498_002926"
     CDS             <1..544
                     /locus_tag="ON498_002926"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3941128.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="ON498_002927"
     CDS             complement(1017..1589)
                     /locus_tag="ON498_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941129.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="ON498_002928"
     CDS             complement(1605..2210)
                     /locus_tag="ON498_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR3941130.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAJSVD010000211         2334 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSVD010000211 DAJSVD010000000
VERSION     DAJSVD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.217"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(105..386)
                     /locus_tag="ON498_002929"
                     /pseudo
     CDS             complement(105..386)
                     /locus_tag="ON498_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            848..2230
                     /locus_tag="ON498_002930"
                     /pseudo
     CDS             848..2230
                     /locus_tag="ON498_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          850 a          396 c          387 g          701 t
ORIGIN      
        1 ttttgaattg tttgccttta aaatgattca ttactctgtc ctctctgtct tttttctcaa
       61 ttttacacta aaatagattt tttggaaaac tttgcaacag aaccgaaatg agtatagtat
      121 gaattagtaa tttggttttc atctaagttt agctttgatt tactaaaatt gatatctcga
      181 tacatgttca atacatctaa tacaaatcga gaatcttctt tcggaaatgg ctcagaaaga
      241 tcactccaaa tttcatcatc ataatgatac tcgtaacctt ctctcaatat ttcgattttt
      301 ctttcataat aagatctgtt ttggtcctca aatttagcca ataactcaaa ttgatttact
      361 agtgacaatc tctgaaaact gtccattaaa tgtttcttcc tatcttgttc attaacaaaa
      421 aattataagc ccttacaacg aatattatat attaaattta attgtaggtg aacacgaatt
      481 ttataaatat ttatggtgtt aatgacaaat gatatccgac tcctcttcat acgttctgga
      541 ctatttgcta atagattaaa atttcagcat ctatttctga ccataaataa atgagctgcc
      601 gaaaagctgc caaatcagac ttgaaaataa aggatcaaaa acttttaagt ttctgacccc
      661 gtaactcaca cttcaaaatc ttacttaaaa aggagtctga aagaaaaaaa gagtattcaa
      721 aatttttttc taactagcta catcacatct atgcattgac actcaaagaa ccagaataag
      781 tcatcagaaa gcaagggggt gcggacataa tcctggacga aaaaataaac ttaggaggaa
      841 tgtccgtgtg ttttcccaca aagagcgtgt aagggcaatt cagttatttt taaaatacga
      901 ttgctcttat gctgcaacca ttcgagaatt agggtatccg tcgattggtg cactacggaa
      961 atggtataaa gagtatttgg aatccggaga gctccatcag gagcaccgaa aaaaatcaaa
     1021 gtattctgaa gaacaaaaac gaatagcggt gaatcattat tttgaatatg gtcaatgtta
     1081 tgcacgaacc attcgcatgc ttggttatcc taatagagag tccttgcggc agcggtgtga
     1141 agaacttgca ccaggagcta gaaaactccg aaaaagtgcg gtaaagttaa cacaagatca
     1201 aaaagaagct gtgttgaaaa aattctataa accgcaaact aataggaaaa atttagccga
     1261 atctggagga attagtagag ttactctata tcaatggaaa aacacggttc ttggaaagga
     1321 ttttccattg tgtatgactt taaaaaatca ggaaaaacaa aaagagctac ttttaaagga
     1381 agtagaagac ttacaaaaac aagtacacca attacagctc gaaaaagcat tactcgaagg
     1441 agcggctgaa ctgctaaaaa aagaaaaagg cgtcaatctt ctttgtctat caaatcaaga
     1501 aaaaacgata ttgattgatg ccttacgaaa tcagttcacc ttgaaagagc ttcttcaaca
     1561 gctccagttt cctaaaagca gctattttta tcaaaaacaa gcacttgaga aaccagataa
     1621 gtattacaaa gaacgtcaaa ttaattatca cgattttcaa tcataacttc tgtgcgtatg
     1681 gttaccgtag aattcaccaa gctttaaaaa atatgggtaa aaagctttct gaaaaagttg
     1741 tacgtagatt gatgactgag gaaaatctat ttgttaaatt ttctagacga aaaaagtatt
     1801 cttcgtatgc tggggaaatt tctcctgcac accccaattt attaaatcgg aattttaaag
     1861 caaagaagcc aaatgaaaaa tggttaacag atattactga atttagaatt ctcgcaggga
     1921 aaatctactt atcaccactt gtcgattgtt acgatggtgc tattgttagt tggactattg
     1981 gaacaaagcc aacttgcttc actctatgtc taaaaagagt tattcaccag ataattcagc
     2041 gtgcgaaggt ttctttggac gattaaaaaa cgagatgttt tatcaacgtg actggaagaa
     2101 tacaacaatc aatcagttta taaacaaatt ggatgattat attcattggt ataataatca
     2161 acgaattaag ttatctttag gaaggctaag ccctcttcaa tatcggaaga agcaaagata
     2221 tatttgttaa aaaggtccaa aaaaaatatc cgcaccccct tttttttgta aaaaactatt
     2281 gcacaataca agtagcctac ggctatcgaa atttccataa ctttcgagca agaa
//
LOCUS       DAJSVD010000212         2314 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSVD010000212 DAJSVD010000000
VERSION     DAJSVD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.218"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            192..446
                     /locus_tag="ON498_002931"
     CDS             192..446
                     /locus_tag="ON498_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3941131.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ON498_002932"
     CDS             447..764
                     /locus_tag="ON498_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3941132.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="ON498_002933"
     CDS             1323..2258
                     /locus_tag="ON498_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941133.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          846 a          313 c          435 g          720 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga gaaaaatgtc
     2281 taaacatgtt ggtaaatcaa caaaaaaacg cata
//
LOCUS       DAJSVD010000213         2296 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSVD010000213 DAJSVD010000000
VERSION     DAJSVD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.219"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            132..1745
                     /locus_tag="ON498_002934"
     CDS             132..1745
                     /locus_tag="ON498_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3941134.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          717 a          407 c          447 g          725 t
ORIGIN      
        1 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
       61 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
      121 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      181 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      241 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      301 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      361 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      421 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      481 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      541 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      601 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      661 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      721 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      781 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      841 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      901 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      961 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
     1021 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1081 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1141 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1201 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1261 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1321 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1381 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1441 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1501 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1561 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1621 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1681 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1741 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1801 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1861 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1921 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1981 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
     2041 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
     2101 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
     2161 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
     2221 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
     2281 gttcctattt atttaa
//
LOCUS       DAJSVD010000214         2291 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSVD010000214 DAJSVD010000000
VERSION     DAJSVD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.220"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(1016..1306)
                     /locus_tag="ON498_002935"
     CDS             complement(1016..1306)
                     /locus_tag="ON498_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941135.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1514..2205)
                     /locus_tag="ON498_002936"
                     /pseudo
     CDS             complement(1514..2205)
                     /locus_tag="ON498_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          655 a          434 c          334 g          868 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat ttaggtttac
     1501 ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg
     1561 tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt
     1621 tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgattt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg t
//
LOCUS       DAJSVD010000215         2240 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSVD010000215 DAJSVD010000000
VERSION     DAJSVD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.221"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            51..758
                     /locus_tag="ON498_002937"
     CDS             51..758
                     /locus_tag="ON498_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941136.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            1139..1318
                     /locus_tag="ON498_002938"
     CDS             1139..1318
                     /locus_tag="ON498_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941137.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1449..1718
                     /locus_tag="ON498_002939"
     CDS             1449..1718
                     /locus_tag="ON498_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3941138.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1711..1968
                     /locus_tag="ON498_002940"
     CDS             1711..1968
                     /locus_tag="ON498_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3941139.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          825 a          307 c          463 g          645 t
ORIGIN      
        1 tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata atgaataagg
       61 tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaaacaaa gaacacttga
      121 atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta
      181 ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac gagtcaaacg aaggttacag
      241 tcagccataa tcgtttagac gtgtataaac gtcgtcctga cacattaaaa aaaggagcgg
      301 agcttctacg actagaaaca gaaaaatata tcatgaatgt ttacctagaa aggagtgagc
      361 tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca ctattactca gaatcagaat
      421 attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca
      481 aagatgcgac ggtagacgat tattaccttg tatttactag atttttgacg tgtgggacgg
      541 aatctatcgt gttaatggga atggattatg aactcactgt acccgaagca tttttgaggg
      601 aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta actgattttt
      661 tagacaccta cacttacgac gatacagaag aaatccaaaa tgaacttgac cgtaaacgaa
      721 tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg aaaagtgagg
      781 atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa
      841 tcgatccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag cccgataacc
      901 taagagaaag aaggagatcc atatgaataa aatcattcct ctattgttcg tactgtttgt
      961 tggcgagata cttatgcgtc atggtgcagc tctacattta aacttcgtag tattcttagg
     1021 aatactggta ttttgtttga tcttttggcg ctttgtgtta acccttgtga gtctaatatt
     1081 caagttgatt gcagccatat ttttaatagg gttatttcga tgaaaggaga gagtactaat
     1141 gccaattgca ataggaaata aacggttacc cgtgacgttg gatgaaaaga gacaaaaaga
     1201 attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc aggattatgt gtattgcgtt
     1261 agatttattg attgcccaag aaaaagcagg atttgaggta ccagcactca aaaagtgacg
     1321 tcacttttta tcctaaaaac taaaagtgat agcactttta attataagaa gttagaatat
     1381 taatcatttg cttaattgta caatataatg tacaattgtt ttatagaaat aaataagggg
     1441 tgaaaggaat ggaagcagta gcttattcaa atttccgcca aaatttacgt agttatatga
     1501 aacaagttaa tgaggatgct gaaacactta ttgtaacaag taaagatgta gaagatacag
     1561 ttgttgtatt atcaaaaaga gattatgatt ctatgcaaga aacgttgaga acactttcta
     1621 ataattacgt catggaaaaa attcgtcgag gagatgaaca attctccaaa ggtgcattta
     1681 aaacacatga cttaatcgag gttgaatctg atgattaagg cttggtctga tgatgcttgg
     1741 gatgattatc tttattggca tgagcaagga aacaaaagca atataaaaaa gattaacaag
     1801 ttaataaaag atatcgatcg ttcccccttt gctggattag gaaaacctga gccattaaag
     1861 catgatttat ctggaaaatg gtccagaaga attacagatg aacatagact gatatataga
     1921 gttgaaaatg aaacgatatt tatttattct gcaaaagatc actattaacc aatcggaagt
     1981 aaggaaaggg tcagaaactt aaaagttttt gatccttatt ttatttaccc tagtcattta
     2041 aaaagctaat atagcttagt gttgattgtt attaatgaat gtgtttgtta cgcgtattac
     2101 ggatataagg ttagtaaaat catttctaaa gttgaggaaa agtaaatata aatggcttaa
     2161 atttcaacaa tttgaagttg aatagatatg ttataatact attgtagtgt gggatgttag
     2221 ttactaaagg atgacgctta
//
LOCUS       DAJSVD010000216         2159 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSVD010000216 DAJSVD010000000
VERSION     DAJSVD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.222"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..461)
                     /locus_tag="ON498_002941"
     CDS             complement(<1..461)
                     /locus_tag="ON498_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3941140.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(745..1032)
                     /locus_tag="ON498_002942"
     CDS             complement(745..1032)
                     /locus_tag="ON498_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3941141.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(1022..1351)
                     /locus_tag="ON498_002943"
     CDS             complement(1022..1351)
                     /locus_tag="ON498_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3941142.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1428..1700)
                     /locus_tag="ON498_002944"
     CDS             complement(1428..1700)
                     /locus_tag="ON498_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3941143.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(1700..1963)
                     /locus_tag="ON498_002945"
     CDS             complement(1700..1963)
                     /locus_tag="ON498_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3941144.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT          671 a          416 c          279 g          793 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa agaagccgtc
      721 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
      781 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
      841 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
      901 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
      961 tttcaatggc ttctctttta gcttttttaa atacttaatg acatgttttg aaggaacgat
     1021 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     1081 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     1141 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     1201 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     1261 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     1321 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     1381 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     1441 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     1501 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     1561 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     1621 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     1681 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     1741 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     1801 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     1861 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     1921 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     1981 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     2041 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttatacccg tggtgctagt
     2101 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcata
//
LOCUS       DAJSVD010000217         2130 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSVD010000217 DAJSVD010000000
VERSION     DAJSVD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.223"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..586)
                     /locus_tag="ON498_002946"
     CDS             complement(<1..586)
                     /locus_tag="ON498_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3941145.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRI"
     gene            complement(620..670)
                     /locus_tag="ON498_002947"
                     /pseudo
     CDS             complement(620..670)
                     /locus_tag="ON498_002947"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            complement(780..>2130)
                     /locus_tag="ON498_002948"
     CDS             complement(780..>2130)
                     /locus_tag="ON498_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HCR3941146.1"
                     /translation="SGKSLEALELEQEEIRRFQNCSLYPVYHGSAKSNIGIEQLIEVI
                     TNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKIT
                     EMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTV
                     EPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYH
                     VEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSL
                     GYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVL
                     EQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIP
                     ARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          668 a          433 c          339 g          690 t
ORIGIN      
        1 ttatccttac ttctttcttt aataatttca taagaaacgg aacagtgata attgttatca
       61 taggaatgag tagaagatag gaccaatgaa tataatgggc tatcattcca ccaatcgctg
      121 gaccgactcc ttctcccatg gctactatcg atccaataag accaaatgct ttacccctat
      181 tttcctttgg aatatagcgc gcaactacaa ccattacgag tgctggaaat gcagctgcac
      241 cagccccttg aataaaacga gccataataa gtaaggaaaa gaaagaatgg ccaacaaacc
      301 caattaccga cccgaaacaa tttattataa ttccaaatag gagtaacctt ttgatgccta
      361 attgatcaga tagctttcca tatacagctg ttccaatgga aaaggttaac ataaaggctg
      421 tgttcaccca gtttgtactc gcaggtggtt tattaaaatc atttgcaata tcaggtaatg
      481 agacgttcaa aaccatttca tttaatacgc taaaaaaaga taaaatgcaa agccaaatta
      541 aaatttggtt gtgtcgtaaa ttcgattgtg aataggatgt attcacattt caccctccaa
      601 taatgagggc agacgtagtt tatagggtta atgatacgct tccctctttt aattgaaccc
      661 tgttacattc atttcacttc ataattaatt cctcctttac actttaattc aaatctttat
      721 taaaaaatat ttcatcttat ttaacaagaa accatattta tataacaaca taaaatacac
      781 taagttattt tattgaacat atatcgtact ttatctatcc gactatttgg acgacggggc
      841 tggcaaacag gttcaccggt agtaacatgg taccctttta actctgttaa acaaacacta
      901 cgtccatttg taaagaaagt taaatcacta cgatattctt gaatacaccg agcagggatt
      961 tctccactaa gaatgacctc attatttttc aattgagtgt ctacgatgtt cgcacaatat
     1021 ttaggagcat cgttgtatgc tcgtgaaaga tattcctgtg gcgcataaat tttaaaacta
     1081 agatatggct ctaacaattc tgttccagct ttttttaaga cttgttccaa tacaatagga
     1141 gcaagcatcc gaaaatctgc tggggtacta acagggctat agtataagcc atacttaaaa
     1201 cagattttac aatccgtcac attccaacca tataatcctt gttcgcaacc atagcgtatc
     1261 ccttccataa ctgcattttg aaatgattga tttaagtatc caagagaaac cgagctctca
     1321 tactgcattc cacttcccaa cggaagcggt gatacagata aaccaatgga agcccagaaa
     1381 ggatttggcg gcacttcgat gtgaatggta tattctgcat tttttaacgg tctctccata
     1441 taaatgactg taggctcttt tagttctatc tccacatgat acttttcttg caacagtgca
     1501 ctaatcactt ccatttgtac tttccctaag aaagaaagta taatttcatg tgtcgtagaa
     1561 tccacataat attgtagaag cgggtcactg tcggagattt ctaaaagtgc atcaagtaac
     1621 atttcccttt gttgaggttt gctcggttca acagttgttt gcagcagagg gagcggattt
     1681 tcaattctct ctctctgtgg caatagcttt gtatctccaa gaacactatt tagcttcaaa
     1741 aactcatttt gcaaaataac aatttccccg gaataagcct tatcaatttt acataattca
     1801 ccatttattg aagtatacat ttctgtaatt tttatttttt ccttttccga tattctaacc
     1861 gaatcccgca aatgcaggat tccgccataa aggcgtacat atgcaagacg ttgtctttct
     1921 tccgaatatt caattttgaa gacatttccg caaagttcag acttctttct gtatgttgat
     1981 gaataaaatt tattcgttat cacttctata agctgctcaa tccctatgtt gctttttgcg
     2041 cttccatgat aaacagggta caaggagcaa ttctgaaatc ttctgatttc ctcttgttcg
     2101 agttctaatg cttctaatga tttaccggac
//
LOCUS       DAJSVD010000218         2094 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSVD010000218 DAJSVD010000000
VERSION     DAJSVD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.224"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..153)
                     /locus_tag="ON498_002949"
     CDS             complement(<1..153)
                     /locus_tag="ON498_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3941147.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ON498_002950"
     CDS             complement(475..696)
                     /locus_tag="ON498_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941148.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ON498_002951"
     CDS             complement(794..1039)
                     /locus_tag="ON498_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941149.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ON498_002952"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ON498_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          699 a          370 c          305 g          720 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aaga
//
LOCUS       DAJSVD010000219         2042 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSVD010000219 DAJSVD010000000
VERSION     DAJSVD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.225"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(77..319)
                     /locus_tag="ON498_002953"
     CDS             complement(77..319)
                     /locus_tag="ON498_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3941150.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(351..908)
                     /locus_tag="ON498_002954"
     CDS             complement(351..908)
                     /locus_tag="ON498_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3941151.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(921..1109)
                     /locus_tag="ON498_002955"
     CDS             complement(921..1109)
                     /locus_tag="ON498_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3941152.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1122..1688)
                     /gene="amaP"
                     /locus_tag="ON498_002956"
     CDS             complement(1122..1688)
                     /gene="amaP"
                     /locus_tag="ON498_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3941153.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT          566 a          411 c          311 g          754 t
ORIGIN      
        1 aaaaagaacc gcagcgagtg tcgctacggt tctcaaattt gttcttcatt tcaacataag
       61 tttttcttca atttgcttat aatttacgtg caaagaatga aacgactaat actaaaatga
      121 ttgcaccgat caatgatgga atgatagcca ttcctgcaat tgttggtccc caagttccta
      181 gtaaagcttg tccaattgct gaacctacta aaccagcaat gatgtttgcg atgatcccca
      241 ttgatgatcc tttattcgtg attgctcctg caattgctcc aatgataccg ccgacgatta
      301 atgcccaaat aaatcccatt ttaaattcct cctaactgac ttcttacttc ttattgtaca
      361 cgtggttcgt tattttcttt gactttttgt gcacctttgc ctacagcaga ttttgcttta
      421 tctgtttggc gtgaagcaaa ttgtcctgtg gattttgctg cttctgttac tttgtcttgt
      481 actgtttctt gatctttttg gtattcttct tgtgttttga tgtctgcaac attcacattc
      541 acttcgataa catctaaatg tgtcatattg tttacttcag tactgatcaa ttctttgatt
      601 tggttgtaaa tatcttcgat gtctttaccg tattctacga tcacatctag atccacagct
      661 acttgtttct tacctacttc tgtattgata cctgctgtta cgttatctgt gttgactaat
      721 ttttcagcca tatttgagaa aaatccgcca tctaatgtca atagaccatc tacatctttc
      781 aatgcataac cgatgatttt ttggatgact ttgtcttcat aagtcaattc accattcatc
      841 atgcggtttt ctcctttttc atttgccaaa actgatccat ttgttgtatt catgtttttg
      901 ttttccatct ttgtttcctc ctatgattta aaaaattgat caactattcc tgactgtttc
      961 aaataaaaac cgattcctgc gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa
     1021 aatccaatcg ttaaccataa aacagctagc agtagtccaa gacccccaaa gataataggg
     1081 agcttgtatg ctgaaaatag ttctttcatt atttggccct cctattccac tcgagagtgc
     1141 ttgtagtctc ttgtatcttc ttgttcttca taaccgctgt atgtgactgc cactttgacc
     1201 gtttcttttg ttccgagtac tttagttact tcttcttcga tatcttgcat caatacacct
     1261 gttttcccta ctaatgaaga agttcgtttc aagtctccgt tgacatggat atggattcga
     1321 tttttcgttg aacgtatccg tactttaggt gaatgaatga actcttcttc gtgtaaatgg
     1381 gaacgtacca acccttcgat cgcttttttg tcgattgtta gtttgccatc ttcccgtttc
     1441 ataacgaaag ttcctcttga ctttggatag aaaagaacga ctagcaagat gataatagca
     1501 agaacggcaa atactacagc tgcccagaaa atatattgtt gcatgtagta attcgttaaa
     1561 gtaaagaagc gaaatcgttc caattgttca ggcataatat acggcgcatt gatcaacgca
     1621 atcgctagaa atactgaaag tagcagtaaa ctcatgataa caccgattat ttttaacccc
     1681 ttattcattt tccacctcct ataaacttat ttttaccctg taaataactc taccgacaat
     1741 cacagtatag atagcgaaca gttagcatag catcagtaaa taaagcgcta ccaaacattg
     1801 ctggatcata acggcgtttt attgccagtc tgatactgaa ataacaattg cttacttatt
     1861 ctctatttac ttttgattgc tggctatact taaaggatat tattcaagat taaaaattgc
     1921 aaagattatg ctcagttatt attacaagat tctatttatt tattaaattt tttcggagaa
     1981 ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta tttttatatt
     2041 at
//
LOCUS       DAJSVD010000220         2032 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSVD010000220 DAJSVD010000000
VERSION     DAJSVD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.226"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            200..742
                     /locus_tag="ON498_002957"
     CDS             200..742
                     /locus_tag="ON498_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941154.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ON498_002958"
     CDS             898..1347
                     /locus_tag="ON498_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR3941155.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2032)
                     /locus_tag="ON498_002959"
     CDS             complement(1377..>2032)
                     /locus_tag="ON498_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR3941156.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          733 a          319 c          388 g          592 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc ga
//
LOCUS       DAJSVD010000221         2010 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSVD010000221 DAJSVD010000000
VERSION     DAJSVD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.228"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            23..1201
                     /locus_tag="ON498_002960"
     CDS             23..1201
                     /locus_tag="ON498_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3941157.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSSMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            complement(1406..1696)
                     /locus_tag="ON498_002961"
     CDS             complement(1406..1696)
                     /locus_tag="ON498_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3941158.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          720 a          356 c          317 g          617 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa aacgtcaaac
      541 cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca ctaaagaagt
      601 actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc tgctacagga
      661 tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct taaaaggcat
      721 gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattgtt gtatccatgt
      781 atctcgtaat atcgctcata aagtacgtgt caaagaccga aaagaaatct gtgatgactt
      841 taaggctgtt tatcaagcta actcaaaaga agaagcgaat accttcttat ccagcatgat
      901 tgagaaatgg aagaaaaact atcctaaagt gacgcagtca ctcatagaaa accaagactt
      961 attaactttt tatgattttc cacctagcat tcgtagaacc atttactcaa ccaatctaat
     1021 cgagtctttc aataagcaaa ttaaaagata cagccgtaga aaagagcagt ttcaaaatga
     1081 agaatcacta gaacgctttc tagtcagcat ttttgataca tacaatcaaa aatttctaaa
     1141 cagaagccat aaaggttttc aacaggtaac cgatacatta gtttcaatgt ttactgagta
     1201 actaattatt ttgcaggagg acaatttatt tacacaaaat tattgacgct ccctttgcga
     1261 gcagcaacta gaataattta aacattatta aactgtctat attgttacta ataattccct
     1321 tcttatagtg cattttctta gaaaaaaaca atattacgat atcatgatat taagatatcg
     1381 taatattata caattgtgat tgatattata tatgtttttt tattttttta tcatgtttaa
     1441 tcttgaagga atcatattct ttttctaaag acaggagctt ttcataaaca tcagacatgt
     1501 ttaaactaga ggccaggctt aaatcataaa cgaatgaagc aggtaatgat tttattctac
     1561 gttgtcgggt tatccagctt gcaaccgttc cttgtgggta ggcatataac tcacaaaatt
     1621 gttctacagt gattcctaaa ttcttgataa tgtaaacatt gatcgggtgc gagtaaacaa
     1681 atgtattgat agacatggtg cgtaagccta ctttctttta ttattgcgtt tgcaataata
     1741 gtttaacaca cgcattattc tagtatctac tcctatttgt aaaaacaaaa aaggattcat
     1801 agagataact ctatagaatc cgtaaaatac cttatatata taacgggaaa atataaaacc
     1861 taaaattatg ataacctgct gcagcttcta catagaaaga gaagcgctca acaccaacct
     1921 catttatcaa acgttggcca taaccccaaa tctcttctga tagctgatca aaggttatat
     1981 actttagctc atattgctgg ataatgttca
//
LOCUS       DAJSVD010000222         2003 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSVD010000222 DAJSVD010000000
VERSION     DAJSVD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.229"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..378
                     /locus_tag="ON498_002962"
     CDS             <1..378
                     /locus_tag="ON498_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3941159.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ON498_002963"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ON498_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3941160.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="ON498_002964"
     CDS             1859..>2003
                     /locus_tag="ON498_002964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941161.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAJSVD010000223         1969 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSVD010000223 DAJSVD010000000
VERSION     DAJSVD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.230"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            97..1044
                     /locus_tag="ON498_002965"
     CDS             97..1044
                     /locus_tag="ON498_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3941162.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            1174..1914
                     /locus_tag="ON498_002966"
     CDS             1174..1914
                     /locus_tag="ON498_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941163.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          688 a          324 c          422 g          535 t
ORIGIN      
        1 attttccgtt ttttattttg ggctataata aactctgtta ggaaaatttg gtataattga
       61 tagtaagaat gacaacatgt tagaaaggaa gagagcatgc ccactttagc agatgttgca
      121 aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca tcaatcatcc ggaacaggtg
      181 actgacgaat tgaaagagtt agttttctca gcaatggctg aactggatta ccgtccaaat
      241 attgctgcaa aagcacttgt cacgaatcgg tcgcagatca tcaaattatt tatactggaa
      301 gaaatcgaca caactgagcc ctattacatg aatttgctga tgggaatcgc taaacggatc
      361 ggtaaagcgc attattctct ccaattagtt acaaatgatg ccttcgacgt cggttcttgt
      421 gatggataca tcatcacagg tgtcagaaaa aaagacttcg attggatcga ccgtttggaa
      481 aaaccagttg tcttgtttgg ggaaaattct gaaggatatg attatgtaga cagcgataat
      541 gaatatggta cagcaaaagc aacgcaatat gccttagaaa gagattatca aacgattatt
      601 tatatcggta tcgaagtgga cgagcctttt gaaatttcaa gggaaactgg ttatttatct
      661 gtgatgaagg aagttgatct tcctagtaaa atcttgcggt ttccaaatca ttcgacagaa
      721 gtagaaagat atatgacaga aaactgggca gattttacag ataaaacttg ttttgtatgt
      781 agttcagatc ggctagcact aggagcagta agagggatta taaatgctgg aggacacttg
      841 cccgaagaat ttggtgtgat tggatttgat ggggtatttt tagaccaaat cagttcacct
      901 aaattgacaa cgatcaagca agatattata cgtatgggtg aagtatgtgg agaaatgttg
      961 ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc gtcgtttttt tcctgaacta
     1021 gttatcagag agacaacgaa ttaagtctta ttactatata tatatttatg aaattcatcg
     1081 ctattttctt aaaataatca gcgcattgct actgaaaaca atgaatctgt gctattattg
     1141 agaaaagttc catttttgag aaggatggtt tgaatgaaag aaatcagacg aaaacatttc
     1201 cgttttcctg cgtacgatga tgagttaggc gtaaaattaa ccaacgacaa gaagcgagta
     1261 ttatttgacg gacaagaaga tcttttgact gaacagaatc agtccccgtt tgaacagatg
     1321 aaagaatttc atttcacgaa cacgaacacc gagaaaatcc gtcgcaagcg tcgtgcaaca
     1381 aatggattga agtcagctcc gcgtaaggaa aatttggaaa agcataaagg aaaattaccg
     1441 gattataccg gccattacca atcagaaatc acatcgacag gacgaaaaaa actttttggt
     1501 gattcctctc gttctgtcag acaaagtgaa acaaggacag agtccaagca tactacacga
     1561 aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt ttgttcctaa atatgtaccc
     1621 gcatcgatta tcccagaccc tagagaaagc aaaatttctg aggatcattt aatcgaatcg
     1681 atgaaaaaaa cggatgactc ttatctgttg ttcgatacag aaccgacgcc gtatcaagaa
     1741 aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa caaaaacgtt agaggaaaca
     1801 aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta tcttagaacg ctcattacaa
     1861 ggattgatcg atgatcaagg aaactctttg ggagaaaata gctattttaa ataagtgata
     1921 tacaaattta tattcggcta cagttggaaa ggaaacgctg cggcgtttc
//
LOCUS       DAJSVD010000224         1960 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSVD010000224 DAJSVD010000000
VERSION     DAJSVD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.231"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..606
                     /locus_tag="ON498_002967"
     CDS             <1..606
                     /locus_tag="ON498_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3941164.1"
                     /translation="AKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINELNSWADS
                     KKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAV
                     INPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQM
                     KKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            596..1738
                     /locus_tag="ON498_002968"
     CDS             596..1738
                     /locus_tag="ON498_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3941165.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1751..>1960
                     /locus_tag="ON498_002969"
     CDS             1751..>1960
                     /locus_tag="ON498_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3941166.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTS"
BASE COUNT          700 a          255 c          407 g          598 t
ORIGIN      
        1 gcaaaagtca ttattgaaac cttaagatta gaaaataagt taatatatgg attttttgat
       61 gataagcaaa ataaaatgca agatctgaaa tatcttggga aaataaacga attgaatagc
      121 tgggcggata gcaaaaaaaa aatctataaa tttattgtag ctattggtga ctgtgtagta
      181 agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc aatatggaat agctgttcat
      241 ccaagtgcta tagtggcaaa agaaatttca attggttatg gaactgttat ttttgcgaat
      301 gctgtaatta acccagatgc tgtaattgga gagcatgcta taataaacac aggtagcatt
      361 gttgagcatg attgtagaat taataactat gttcatttat ctccaggtgt atgcttagcg
      421 ggtggagtac atgtaggtgt aggaactcaa ataggaattg gttctcaatg tatccagatg
      481 aagaaaattg gatcttggtg tatgataggc gcagggagca ctattgttaa ggatattccc
      541 tcacattcct tagcttatgg caatccagct aaaattaaga aagaaggaat taattttgaa
      601 ttctagaatt ttattatcaa gtcctcatat gggaggaaca gaagaaaaat atgttgctaa
      661 ggcttttaaa gataattgga tagcgccatt aggtgaaaat gtggataaat ttgaagaaag
      721 tattacaaaa tatactggtt ctaagggcgg agcttctgcg ttaagttcag gtacagctgc
      781 catacatctt gccttaagac tgcttaatgt tggaaaaaac gatattgtct ttgctccttc
      841 atttactttt atagctacag taaatcctat tttatatcaa ggggctgtac cagtgttcat
      901 tgactcagaa cctaatacat ggaatatgag ccctgttgca ctagagagcg cattagaaga
      961 agctgaaaga aacaaccaat tacctaaggc aatcatcgta gtgaatttat atggtcaaat
     1021 ggctgattat gaacgaattt tagctattgc aaaccaattt aatgttccag tgatagaaga
     1081 tgcagcggaa tcgttaggtt ctacttataa gggaatttcg tcaggcttat ttgggacaat
     1141 tgggatctat tcatttaacg gtaataagat catcacaacc tcaggtggag gagctctagt
     1201 gtctagtgat aaaaaatatg ttgagcattc aagattttta gcaacacaag caagagatca
     1261 agcaccgtat tatcaacata gcgaaatagg ttataattac cgtctaagca atatttctgc
     1321 cggtatcgga cgtggacaaa tggaagtatt gaatgataga gtagctaaac gaagagaaat
     1381 tttcaatcga tataaggaag ctttttctgg aattgataga attgaaatga tgccagaagt
     1441 ggatggcatg gtgccaaatc gttggttgtc tactttaacg atcaatgatt ataattctat
     1501 tgatcaagta gttaacgtaa taaataaact aaatgaaaaa aacattgaaa caagagcttt
     1561 gtggaaacca ctacatttgc aaccagtatt tcaaggggta aaaatgtatt accatgataa
     1621 taatcttagt gtgtcagaaa agttatttaa tagcggctta tgcttacctt ctggaagtaa
     1681 tatgacagat agtgaacaag aatttgttat tgatgagttt aaaaaaatat tcaagtaggt
     1741 gtgatatttt atgaacatag gaattattgg tttaggttcc atggggaaaa gaagacttag
     1801 actcattcaa gaacacttta atgatatttt actatgtggt gttgatacta atgaggcgag
     1861 acgaaaggaa gttgaaaagg aatttggaat accaacttat aatagtctaa cagaggcaaa
     1921 aaatttgttt gaaataaatt cagtttttgt ttgtacatcc
//
LOCUS       DAJSVD010000225         1942 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSVD010000225 DAJSVD010000000
VERSION     DAJSVD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.232"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            319..936
                     /locus_tag="ON498_002970"
     CDS             319..936
                     /locus_tag="ON498_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941167.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            977..1276
                     /locus_tag="ON498_002971"
     CDS             977..1276
                     /locus_tag="ON498_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR3941168.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1310..1477
                     /locus_tag="ON498_002972"
     CDS             1310..1477
                     /locus_tag="ON498_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3941169.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1522..>1942)
                     /locus_tag="ON498_002973"
     CDS             complement(1522..>1942)
                     /locus_tag="ON498_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941170.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          653 a          309 c          379 g          601 t
ORIGIN      
        1 taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt gaaaaataaa atataccatg
       61 gaagacataa aagaaaaact ggataactag tttatttaat ggcagaccgt taaggcctgc
      121 catttttatg tattttagcg gaataaatat aatcatgcgg gagtgtaaac ttactgtttc
      181 aaaaagtata ctttttgaca tgccttgatt ttctttgttt atgtatggca taataaccac
      241 ataatacaat ttgaaacgtt tctgatttaa tctgattgtt ttgaaacaat tttagtgaaa
      301 aaagggtaga taaaaacgat gagaaaattt ttttatatcc gggtcagtag ttatgatcaa
      361 tcgatcgaac gacaactgat ttccgcgaag gaattaggaa tccctgaaga atatatattt
      421 gtagaaaaag ccagcggcaa agattttaaa cgccctgaat atcaattaat gaaacggatg
      481 tttcgaaaag gagatttact ctatctacaa tcattagata gactaggcag aaataaacaa
      541 atgattttag aagaatggcg agaattaact aaggaaaagc agattgatat ttctgtgtta
      601 gacatgcctc tgttagatac tgctagttac aagcaactag atgggttaga gacgcttatc
      661 tctgacttaa ttctgcaatt actcagctat atggccgaag acgagcggaa acgaattaat
      721 agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg gtgtaaaatt cggcagaaag
      781 aaaattgctt tacctcctga atttcctgcg atctattctg actggaaagc aggaaagata
      841 actgcagtct atgctatgga tcaattgggc atgaaacgaa atacctttta tcgacgggtg
      901 aaagaatatg aacatacctt gcaccacttt tcttgataga tataacagct attagacggg
      961 aagggagagt ttgaaaatgg acttaacgaa tcaaagggtc gtttttactg gaacgctgca
     1021 gcaaatgaca cgaacccaag ccatcggttt ggttcactct ttaaacggcc attgtcaatc
     1081 aagtgttact tcaaaaacaa atttcctggt ttgtggtcag tattctaagt cgttgtttga
     1141 tgattctctc tatacaaaaa aggaacaaac cgcgcgggac ttgattgcgt taggtcatga
     1201 gattaccatt ttatcagaag tcgaatttta tgcgttaatt agtcaacagt tgaaagaatg
     1261 gcaacggtat ttataatcgt tagtaaaatt tacaaggagg aaaattttta tgaagcgaaa
     1321 atcttatgat cgttggggca ttgaaataac tagttttaat cgaaatattg cttttcacgt
     1381 ggagtgtgac tgtggggaat tagcaaaaag tgtttatcaa aaaaattact tttactgtcc
     1441 taaatgccat cgccgatacg gggtacatgg aacatagaaa cgaaatagtg cggtagaaaa
     1501 gcgaatgaat gtagtatatt tctacaaatc attcgctttc tttattttat aaatagttgg
     1561 tcgagagatc ccgttttctt ttgcaatctc tgcaactgga atgccttgct ttaacatgtc
     1621 caccacggtt ttaaaaacta agcgtttttg cggatctgtt gagttaatag aatactcgat
     1681 tggtcgtccc ttatatcggc cggctttttg agccaataat actccttgcc gctgacgttc
     1741 acgaattttc tcacgctcgt tttgtgcaat ataacttagc agtgataaaa acatatcgaa
     1801 catagcggta tccaggagtt catttccagt attaaaatct aaaaactgtg catctaatat
     1861 ttttaacgct acttttttct gtttcataaa agcaactgta ttttttatat cttcataatc
     1921 tcttcctaaa cgatctagtg at
//
LOCUS       DAJSVD010000226         1922 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSVD010000226 DAJSVD010000000
VERSION     DAJSVD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.233"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            140..>1922
                     /locus_tag="ON498_002974"
     CDS             140..>1922
                     /locus_tag="ON498_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR3941171.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFA"
BASE COUNT          744 a          263 c          336 g          579 t
ORIGIN      
        1 gatataaata ttgcaggctt ttttattgat ttgaaataat caagaatatt agaaacttct
       61 acattaatca agaactttct tctgtatgaa aatagagata ataattgtac acttaagctg
      121 tattcaggag gtgaagataa tgggcatcga atttagcgaa cggcaaatga aaatactata
      181 ctttttgatt gaaaatgagc acacaactta caaagaatta tcagaacaat tatatgtatc
      241 tacgaaaaca attagcaaag atattttaat tttgaaagaa ataatccaaa aaatgccaga
      301 tgtacaaatg ttggttcaac ctaaaaaagg cgtttcgctt attggaaaaa aagaaacact
      361 tcgaaattta ttatccagtc ataaaatcaa tatcgcagac tctccggagg accgtctaac
      421 ttatgtgttt gctaaattat tgaatactaa tgattctcta aagattcaaa atcttgctga
      481 cgaattatac gtgagcaggt cgacggtaga aagcacaatg aaagatgtta gaaaaagact
      541 aaacaagcat gggatgtcct tagtttcaga tcgaaatggc gtgcgcatca aagctgatga
      601 aaaaagtaaa agaaggttga tgtcagaagt tgtgaactat tattggggtg gctttacagc
      661 atctaacagt aaaacagatc tcttagactt gaatatcaat ttcattggtg aagcgaataa
      721 tttcttaaat aaggacatat tgaaaaaagt agccgaattg cttaatcgat acatgaataa
      781 gacacaattg aaatttacag attatgaatt tcaaacttta gctatccacc ttgctattgc
      841 gatagaaagg attgaaacaa gcaatttctt atcacgtagt aataattctg aagaagaaat
      901 ctcaaagaat acacttattt taatcaaaat gattgaaaaa gcattttcaa ttattgtgcc
      961 aatagatgaa caagcttatg ttgacattca cattacttcg attgaaaaaa atgctatgaa
     1021 tcacgactta gatcaaacga agctttccta tttagatatg ggtgttgagc tacaagagtt
     1081 agtaagaatt aaattaaatg aattttatcc tgatgaagaa ttgatcaata atctagtatt
     1141 acatctcaat gcagcagtta aaagactcaa attgggagta aatatctata acccttatac
     1201 ggacaaaatc aaatattctt ttaaaaggtc atttatgata agtgtagatt tattagagga
     1261 aatcgaagag agattctgta tacattttaa tgaagatgaa atcgcttacg taacactcca
     1321 tattcaatca cttttggatc gttacaaacc tgacaaaacg aaagtgatat tagtttgtag
     1381 tagcggatat ggaacatcaa aattattaga gcaaagaata aataacggtt ttgcagatat
     1441 ggttgagata gaagatgtac taagcatcaa tgaactacaa gatagaaaag tcactgatga
     1501 acttgttata agcacgattc cgattgaaaa tacaaatttt cctgttattg ttgttagtcc
     1561 tttgatgtta ggggcagatg tcagaaaagt aaaacagtat ctacagttaa atgaacaaac
     1621 agaagatgct tttctaaatt tattagataa acgattttgc ttttttaccc accaaataaa
     1681 ggataatcaa gtgaacgttt taaatataat taccaaaaaa ttggtaaaag aaaattacgc
     1741 gaaagaaggg atactagagg gagcaatcaa aagagaaaaa ctatcttcaa cagcgatgaa
     1801 tgattttgct atgccgcata tcgaagtaga ttttataaaa aagccagtaa tctgtgtcta
     1861 cgttaatcca gaaggaattg attgggatgg gacacttgtt cattctgttt tcttttttgc
     1921 ac
//
LOCUS       DAJSVD010000227         1885 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSVD010000227 DAJSVD010000000
VERSION     DAJSVD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.234"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            120..638
                     /locus_tag="ON498_002975"
     CDS             120..638
                     /locus_tag="ON498_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941172.1"
                     /translation="MSIEELVCGNVEEYILGIILYRDSIVLDGITFPDKNLFQHLRQQ
                     FPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNILKIEELFINAIVEE
                     FDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNINKLEQTISSIGKK
                     YTKKKMKGGDTI"
     gene            677..1819
                     /locus_tag="ON498_002976"
     CDS             677..1819
                     /locus_tag="ON498_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3941173.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          739 a          294 c          335 g          517 t
ORIGIN      
        1 catttggtct tatgattaac aacggtcaat taattgacaa aaagactatc tacgaatggg
       61 agaagggaac ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata
      121 tgagtataga ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt
      181 atagagatag cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc
      241 ttcgtcaaca gttcccacca gtacactcta atttagatac atggctggat agatattcca
      301 aattagaacc agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg
      361 aaaaaattag tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattgtag
      421 aagaatttga caataacatt cttttcctca ctagcagtat agaagaacta ctagaaagaa
      481 tggtagatga atggctacct attcaattga aagacatgtc ttatcccgaa gaagctgtga
      541 gagaaattac tgataatata aacaagctag aacaaacgat ttcatcaatt gggaaaaaat
      601 acactaagaa aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact
      661 aagaggagat gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc
      721 gtggatgtat aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac
      781 gagaagaggt tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca
      841 aatattatac ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga
      901 atggataact catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata
      961 cgcaaataat caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc
     1021 atattgtcaa aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag
     1081 aaaacagact gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc
     1141 tatggaaaaa acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc
     1201 aaaagaggag cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata
     1261 cgccttcttc agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca
     1321 gtggaaagac atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga
     1381 tgaatttggt aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc
     1441 tttagatact gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta
     1501 tttaaaactt ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa
     1561 cactctatac attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa
     1621 cttgcctcgt attacacctc atgggtttag acatacacat gctagtttat tattagaagc
     1681 aggagaatct gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt
     1741 agacatctat gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga
     1801 tatgatggct catcagtaaa agagctggtc aaaatctggt catttctagt atacaggcaa
     1861 aacaaaaaag ctagaaccct tatat
//
LOCUS       DAJSVD010000228         1873 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSVD010000228 DAJSVD010000000
VERSION     DAJSVD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.235"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(7..549)
                     /locus_tag="ON498_002977"
     CDS             complement(7..549)
                     /locus_tag="ON498_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR3941174.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            684..1073
                     /locus_tag="ON498_002978"
     CDS             684..1073
                     /locus_tag="ON498_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941175.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1077..1841
                     /locus_tag="ON498_002979"
     CDS             1077..1841
                     /locus_tag="ON498_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3941176.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
BASE COUNT          580 a          338 c          372 g          583 t
ORIGIN      
        1 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
       61 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      121 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      181 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      241 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      301 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      361 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      421 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      481 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      541 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      601 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      661 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      721 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      781 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      841 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      901 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
      961 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1021 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1081 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1141 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1201 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1261 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1321 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1381 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1441 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1501 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1561 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1621 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1681 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1741 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
     1801 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1861 atgacgatag agg
//
LOCUS       DAJSVD010000229         1873 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSVD010000229 DAJSVD010000000
VERSION     DAJSVD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.236"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(152..346)
                     /locus_tag="ON498_002980"
     CDS             complement(152..346)
                     /locus_tag="ON498_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941177.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
     gene            594..1735
                     /locus_tag="ON498_002981"
     CDS             join(594..822,822..1735)
                     /locus_tag="ON498_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3941178.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          625 a          389 c          353 g          506 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgatttggta gaatctaact actaagaaaa ggacgtgtcc acgatggcaa
      601 aagccaagaa aaaatttgat gaagacttta agaagatgat cttagattta aaccagtctg
      661 gccaatcggt cgaagaactg gcagcgcaat atggtatcgc tacacaaacg atttatcgtt
      721 ggaagaagct tcatacaaag aatgtagcaa ccggcatgac tgaggcagaa atcctagcga
      781 tgaaaaagga aatggcccgc atgcaggagg aaaacactat cctaaaaaag gctttaacca
      841 tattcgctca aaagtaaaaa tgagtgatgt cttcgaattt attcaatcaa atgttcatga
      901 tcatgacgta aaacaaatgt gtaccgtttt ggaagcaccg aaatccagct actatgactg
      961 gaagaaaaag aaaccttcca agcgccaatc agaaaacaaa cgcttaaacc gcttgatttc
     1021 aggaatctac tttgaaaaca agggtatcta tggtgcgcct aaaattcata agattcttgt
     1081 tggccgtggc gagaccttgt ccttaaagaa agtacaaata ctgatgagaa aattaggctt
     1141 gcgttcagtc accctcaaga agtacaagcc tggcaaacaa gcgaaagtta aaagtgaagg
     1201 acgtaaaaac ctgttaaacc aagattttac cactacgtcg atcaatcaaa aatgggtgac
     1261 agatattacc tatattcata ccctaaaaga tggctggacc tacctctcca ctattcaaga
     1321 cctccacaca aagaaagtca tcggttggaa gtttggcaag cagatgacga aagaattggt
     1381 tatcgaaacc cttgacaatg cgctcttaaa ccaaacaccc tccgagaact tgatcatcca
     1441 ctcagatttg ggttctcagt acactagcga agcctatgag gcaaagctga atgaattgaa
     1501 cattcggcac tccttcagtc gtaagggctg tccctacgat aatgccggta tcgaatcgtt
     1561 tcacgcctca ctcaaaaaag aagaagtcta tccatcaaaa gcgtatgaaa actttgaagc
     1621 tgcttattta gcgttatttc aatacattga aggattttac aatcgcaagc gaatccatag
     1681 tagtatcaac tatttaacac caaatcagat ggaggagttg gcattgcaag cttagttcct
     1741 agctaccact tacatttttt tggcgagatt tccacagggt ttcaacttca tcgcttcgat
     1801 cagcggtcaa acggttttgg tttgaccgct ggtcgaaacg atgagatact cacccgctgg
     1861 aatctcgaaa aaa
//
LOCUS       DAJSVD010000230         1821 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSVD010000230 DAJSVD010000000
VERSION     DAJSVD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.237"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(28..1821)
                     /gene="uidA"
                     /locus_tag="ON498_002982"
     CDS             complement(28..1821)
                     /gene="uidA"
                     /locus_tag="ON498_002982"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR3941179.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGKFITQ
                     HKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKENLVN
                     FDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIAIYDE
                     EAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGIRSVQV
                     TNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTVIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     VCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRVQGNK
                     KGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT          553 a          377 c          224 g          667 t
ORIGIN      
        1 tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg gtattgatga
       61 ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag taaagattcc
      121 tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg caaaattcca
      181 cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg cttcgtaata
      241 agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc cagctactgt
      301 atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca tccatccatc
      361 tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa cataccaacc
      421 ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa catctggcgt
      481 tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt ctttagttaa
      541 ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg gttcatttgc
      601 aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt ccttgatcac
      661 cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt ccacattatc
      721 atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt catcaataac
      781 tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat aatgtgatgt
      841 tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat ttacagcctc
      901 attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt taaaataaaa
      961 aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa taccaaattc
     1021 ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca tactatataa
     1081 ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc ctttctttcc
     1141 ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga tttttccggt
     1201 ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc ttgtattaat
     1261 agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc tatgaatacc
     1321 tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc cattttcctg
     1381 tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat tgtttacagc
     1441 aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact caaatggtgt
     1501 aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg cttcatgtgt
     1561 tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa gagtcacttc
     1621 tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa ataaatcatt
     1681 ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt ctaaagcaag
     1741 ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc ttaagtcaat
     1801 tacttgcctc gtctctgtca t
//
LOCUS       DAJSVD010000231         1815 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSVD010000231 DAJSVD010000000
VERSION     DAJSVD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.238"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..871)
                     /locus_tag="ON498_002983"
     CDS             complement(<1..871)
                     /locus_tag="ON498_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3941180.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNV"
     gene            complement(868..1092)
                     /locus_tag="ON498_002984"
     CDS             complement(868..1092)
                     /locus_tag="ON498_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3941181.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1089..1382)
                     /locus_tag="ON498_002985"
     CDS             complement(1089..1382)
                     /locus_tag="ON498_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941182.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1419..1556)
                     /locus_tag="ON498_002986"
     CDS             complement(1419..1556)
                     /locus_tag="ON498_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3941183.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1558..>1815)
                     /locus_tag="ON498_002987"
     CDS             complement(1558..>1815)
                     /locus_tag="ON498_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941184.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          498 a          381 c          289 g          647 t
ORIGIN      
        1 caacatttcc agaagtatta taagcttgat ttacatctga tctataaatt ccatcagctc
       61 ttactcgagc taattctttc ccagtattgt aatccataat gaagatatac tcgtatttat
      121 agttagcaat gtgccatcca gctacatgca aatttgcatt ttcgatttct ccaaactgat
      181 caatgtgggc gtaattcgtt ccatctgtca gtgtaggatt agctgcacca gctcgtgttg
      241 gatcaatgac aggcttgttt tcagaagttg ttggattttc atcggtaaat ccatgagcta
      301 aatcatatgc taatttttct ttacttacgc ccatttcaga aagataaccg taaggatctg
      361 tatgatcgcc ccagatgttt tgtgttaccc ataaatgcga tttgattccc ggttggttat
      421 aaggagtgtc caacgttaat ggaataccat atttcattgc tgaatctcta gccaattcaa
      481 cgtatgcctt gtagtttttc tcaaacgttg ctttatcatg tgtgtgttgt aactcaatct
      541 gcacaggact gttggcatta gcatacgaac cagcaccgta ctgtacataa ccaggttgac
      601 cgacttgata aacaattccg ccgtctccca caatataagc agtataagcg ctagtccatg
      661 aacgttgcat atactgcgct tcattgcgtc ctgttgctgt ttcattagcc gtttcatgca
      721 gtaaaatata ctgattattt gctacttgtg agctaccttc atttgcgccc aaattaaatt
      781 cattgttgat agtataggca aatccgttaa ttggcaataa aaaaagagcc gttaacaggc
      841 tcatcgcagt aatagtaatt ttctttttca tttgtttcct ccttcttcgc tttcagccga
      901 gaacattttg taggttcgat ttgatacacc caacacactc cctaaaaacg cgccaaaacc
      961 agtaatgatg acaacacaga tatctgtgta ctgccaattg agcgctttac caactaaccc
     1021 cacgaaagta gctagtgcgg gaataattac cagtgcgaac cattttagta cttcgaacgt
     1081 tttattattc attttcttct ctccctaaac aaagttttga tttgttgcgt gtgttccacc
     1141 aatttttctg tatgtgtatc taatctttca tcgtgtttct ttagttcttc atgaatcatc
     1201 aatcgatctg atttgctcga ttctaaatct ttagtcagca aatctaaatt gtggcttact
     1261 tttgaaagag tctcagtaat tttcgagaaa gatgcagcaa tcggtctaat tactaataaa
     1321 atcaaagaaa cgatagcggt tattgatcct gctatcattc cccattcccc taaattaatc
     1381 atgtgacaac tcctttacct taaataaaaa cgcatcaatt aagatgcgct cttatcttta
     1441 ttaatgattt tatctgcttc ttcgtctgta atgcataatg gaacgaaaac cattacttgt
     1501 tcgttagtga aacagcccca gtcatacatt agtttgatat cttcatatga atacatgtta
     1561 cttcccacct tgtaattgtt ttttgatttc ttcgatttcc ttggtatttt gtacagaagc
     1621 aagcatcgtt tttgaattca actgcgctaa actttctgac ttttctttca ataagacatt
     1681 ttcttgcttg attgctacat tgtttagcat tgctttagaa tttagctgtt tcaattcatc
     1741 attttctgcc ttcagcgttt cgtataatgc cttaatatta gccaattcat ctacgctatc
     1801 aacagcattc ccttt
//
LOCUS       DAJSVD010000232         1777 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSVD010000232 DAJSVD010000000
VERSION     DAJSVD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.239"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..382)
                     /locus_tag="ON498_002988"
     CDS             complement(<1..382)
                     /locus_tag="ON498_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3941185.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGN"
     gene            complement(384..1103)
                     /locus_tag="ON498_002989"
     CDS             complement(384..1103)
                     /locus_tag="ON498_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3941186.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT          633 a          312 c          226 g          606 t
ORIGIN      
        1 tatttccaaa taaaatatga aataaatccg ttgagctatt tgcaactcct ataaggataa
       61 ctcctagtgc taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac
      121 tattgctttt tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa
      181 taaaaaaatt aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa
      241 tagcatcacc cataagagac attcctctta aaataataaa acaacctact gttccagaaa
      301 caatccctat aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac
      361 cattaataaa attttgaagc atattattcc ccctctccaa taaatatagt atctccatag
      421 gcttttttta aattttcttc attaaataca gattctacac ttccgtgagc aattagattt
      481 cgattcaaaa gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca
      541 attagcactg tttttccctg atgtttcaat ttttttaacg tatccataat tattcgttca
      601 ctaactaagt ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca
      661 agacatcttg ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga
      721 ttagaatatt ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca
      781 tttttaattc tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt
      841 acttttattg gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc
      901 agttttttat tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt
      961 aacattgctt taagcaatgt ggattttcca gcaccatttg gaccaataat acctgttaaa
     1021 gcaccttctt gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca
     1081 ttaagctttt tcacttctaa cataactttc tccttctaaa tgataatgat tatcacgatc
     1141 atatggtata caaaaattat attttagtca accctaaatt ttatagttta gatttttttc
     1201 tgttgctgaa gattagaatg acaaaactat ttgaaactac gcgtatagta agtagctttc
     1261 aaaatatata aaaaaggagc gttctcaaat gagaacatgc tcctaaaact actttattgg
     1321 cgtatggaaa aattatactt gaagtggata gcgggttgaa taagttgata agcatcaaaa
     1381 catttataaa aattattcaa gactatgtct gttccatata caaatatgaa aatcagtaaa
     1441 ctccatttaa cttttttcgt agtgtgtctt tttatacatg tttatcgtta ttaatagtga
     1501 ataggaaaaa aactattttt gatcattgac aattaaatac aatttttgaa taacgatccc
     1561 tgctattcga gctagaaact caagtacgcc atgacctgat tagcgacaaa tacttagaga
     1621 tttctttttg ttctgggaaa acaaaatgcc atgacaatag cagcaaataa tgatacttcc
     1681 gtaaggctcg ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcaattc
     1741 aaaaatttca taactactat tttaggagat gatttta
//
LOCUS       DAJSVD010000233         1771 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSVD010000233 DAJSVD010000000
VERSION     DAJSVD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.240"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..979)
                     /locus_tag="ON498_002990"
     CDS             complement(<1..979)
                     /locus_tag="ON498_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3941187.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHD"
     gene            complement(976..1395)
                     /locus_tag="ON498_002991"
     CDS             complement(976..1395)
                     /locus_tag="ON498_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3941188.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1465..1605)
                     /locus_tag="ON498_002992"
     CDS             complement(1465..1605)
                     /locus_tag="ON498_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3941189.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1598..>1771)
                     /locus_tag="ON498_002993"
     CDS             complement(1598..>1771)
                     /locus_tag="ON498_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941190.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
BASE COUNT          480 a          379 c          287 g          625 t
ORIGIN      
        1 tgtcatggaa atcttgcgca ccgcctttag tgtttccttc tggatcattc gttgcccgca
       61 tcatgacgta tactttctta ttagggaaat tgcgcatgtt aaaagataca tgatagccaa
      121 cgtttccaga agtattataa gcttgattta catccggtct ataaattcca tcagctctta
      181 ctcgagctaa ttctttccca gtattgtagt ccataatgaa aatatactcg tatttatagt
      241 tagcgatgtg ccatccagcg acgtgtaagt tcgcattttc gatttctcca aactgatcaa
      301 tgtgagaatg gttcgttcca tctgataaag ttggatttgc tgcacctgct cgggttggat
      361 caatgacagg cttgttctca gaagtcgttg ggttttcgtc tgtaaatcca tgagctaaat
      421 cgtaagccag tttttcctta cttacaccca tttctgataa gtatccatac ggatcagtgt
      481 gatcgcccca aatgttttga gttacccata aatgtgattt gattcctggt tgattatacg
      541 gtgtgtctaa tgtaagtgga ataccgtatt tattagcgga atctctcgct aattcaacat
      601 atgccttgta gttcttttca aacgttactt tatcgtgcgt atgttgcagt tcgatttgca
      661 caggactatt tgcattagca tatgaaccag cgccatattg tacataacca ggttgtccaa
      721 cttggtaaac gatcccacca tcgccaacaa tatacgctgt ataggcattg taccaagaac
      781 gtttcatgta ctgcgcttca tttcgtcctg tcgcagtttc gtttgctgtt tcatgcaata
      841 ggatgtattg gttatttgct acttgagaac taccttcgtt tgcgcccaaa ttgaattcat
      901 tgttgatcga gtaagcaaag gcgtttattg gcaataaaaa aagagctata atagctccta
      961 caaagatttt ctttttcatt tggttcctcc tcggtcttta ttttcagtat catttcgtat
     1021 ttgttggaat acctctttta acttatctgg caaaggaagg aataccgaaa tgttttctaa
     1081 aaaactaatc ccttcatttg caacatagaa cataataact atttctctta gaggtataga
     1141 attcccaacg attttctgta cgtcaaccgc aacggcaatc acaacgaaga ttaagacttt
     1201 cttcaacaat ccttcgaaac ctatctcact acttatttct ttcaatttcc atgccttagc
     1261 aagtccagtt aagaagtcca cacccacaag aaaaattaaa acatgtagta tattgtccat
     1321 ccctcccaat aaaccaacaa ttgtcccgcc tatcacccct gtcattactg agagtgtatt
     1381 taaatatttt tccaatatct atttctcctt tatgcaaaga agcgcactcg tttgagtacg
     1441 ctttttattt aactttgtgc ttaattaaat ttcatttcca gtaatctgct tatattggtc
     1501 ttttgttaaa cagttcatgt ttacatagtc aagtaaatct tggtccgtat aacatcccca
     1561 atcataaaat tgttttatat tttcaaagcc cggaaaacta ttcattactt ttccctccat
     1621 tcttaatact tgcgacttcc atcgtcaatt gagctaacat ttgttgcatt ttttcttgtt
     1681 cctgtaccga tatcgccaat tgttttgtca gtagagcatt taattgcttc aactgatcga
     1741 catcttcaat atcgtcaacc gctgtgccac t
//
LOCUS       DAJSVD010000234         1757 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSVD010000234 DAJSVD010000000
VERSION     DAJSVD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.241"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..929)
                     /locus_tag="ON498_002994"
     CDS             complement(<1..929)
                     /locus_tag="ON498_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR3941191.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISV"
     gene            complement(1088..1354)
                     /locus_tag="ON498_002995"
     CDS             complement(1088..1354)
                     /locus_tag="ON498_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941192.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1344..1700)
                     /locus_tag="ON498_002996"
     CDS             complement(1344..1700)
                     /locus_tag="ON498_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3941193.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          510 a          333 c          208 g          706 t
ORIGIN      
        1 ataacagata tctttttttc attttttaaa aaataccttt taaatttctt atagtcctct
       61 gaaatcaaaa tatagcgtga tggatactca tcaaaagaag aatcgtattc ttcctttaaa
      121 attcccgatt tacttaattt ttctagtaca tcaaatactc tatcactcaa gtaactcctt
      181 aaattgttat cactttccca ttgaactact tttctataag ttgaattatc agttgaaaag
      241 atagaatttt catccataaa cgcatatatt agcagcaatc tttcatcatt gtcaaattct
      301 attggatcaa aaatatctaa gttgtcatca aaatctttat cagttccggt aacttttttg
      361 ttaattactg aaggtgtctt gtaagaaata gaatctaaat caaagcgttt tgattctaaa
      421 ttattttgtg ctttctcaat taaataattc actttttttg tgagaaacct tctgatattg
      481 tctttgctat atgttccata aaaaccctta tcttctaaag agtccctgta cttcttaact
      541 tctatatact cttcagccaa aacttgtgga actggttcat ctaagaaaaa aacccctacc
      601 tcgcctttgc tatttttata aaagtaatca atttctgcta tagttcccga tctataatta
      661 tcaactggag aacccaactt atggccaaaa atcgctaata atatatcact attttctaca
      721 atttgtttat taataacact ttgcccagag ctattattta tataagtagg aatcgcattc
      781 gtttcccatc gaattggcat caaaattact tttgtattat ctgtattttc tatattccat
      841 ctatatatgg tttcttctac ttcattcctc tttgctgcta tatcacctgg tgatgctata
      901 aatactttta ttagatttat ggttttcatt tattgtacct ttctctttat gtctctttaa
      961 agtaattttt gtataaaatt tattgacttt tacactatag gacaactttt atctatttag
     1021 tacaaaaagg cccactaaac taattgatta gttaggagcc tattacaggt atattttcaa
     1081 ctcgctttta agcaaattct actaccagct ttttgcctaa tgccctagcg atatcatcaa
     1141 gtttatcgaa agatacgtta tcccctctct caattcgagc aatagtagat tgagttgtat
     1201 gtgctttttg tgcaagctcc gcttgagtta ggcctgcttc ctcccgtgca tgataaagag
     1261 caaccgcagt ttcgagtttt cttccttcac ggtcatatgc ttctgcaaaa tctttgtctt
     1321 tttgcttttc ttcaatcaat tttttaatca tcatttgtga accactcctc tctaagttct
     1381 tttgcatgtt taagttcact tattggtgtt ttctccgttt ttttagtaaa tccatgggta
     1441 atgacatacc ttccattgac cacatggaaa tagatagctc tttggatgtt tgatcctacc
     1501 ttacttctta attcgtacaa attagtgtct aattttttca cccatttcaa ccgttgagct
     1561 actagcatcc cattcttttc tgtttcttct attgtacgta aaagcttcgc actatctttt
     1621 tttggtaatg tttccaacca ttctaaaaat tcatcatgac cattaggtct tttaaatgtt
     1681 tcaaaagata ttttttccat acccaattat atcatatatt gattttatat tatagtatat
     1741 aacataaaaa ttcgaat
//
LOCUS       DAJSVD010000235         1733 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSVD010000235 DAJSVD010000000
VERSION     DAJSVD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.242"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..903
                     /locus_tag="ON498_002997"
     CDS             <1..903
                     /locus_tag="ON498_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR3941194.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1733
                     /locus_tag="ON498_002998"
     CDS             916..>1733
                     /locus_tag="ON498_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCR3941195.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETV"
BASE COUNT          582 a          252 c          396 g          503 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttcaga taatcacgaa gtcgaaaaaa ttgaaacagt aga
//
LOCUS       DAJSVD010000236         1684 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSVD010000236 DAJSVD010000000
VERSION     DAJSVD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.243"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            145..1623
                     /locus_tag="ON498_002999"
     CDS             145..1623
                     /locus_tag="ON498_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3941196.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          617 a          231 c          302 g          534 t
ORIGIN      
        1 caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca agcataatcc
       61 atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag taattggtat
      121 gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga ggaagataga
      181 gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt agtgctgcct
      241 aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat catagaatta
      301 aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga agaagcagag
      361 atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat ctactttcaa
      421 cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac gatcgaaaaa
      481 tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag agaattagtc
      541 tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg ggaagaaata
      601 aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt ttctatgcca
      661 tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga atatctgttc
      721 aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac tattagtttg
      781 ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga atactttaag
      841 agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct tcatatccct
      901 aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac ggaagaagaa
      961 tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat ccacatgatt
     1021 agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga aatctctgaa
     1081 gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt caatcgaaaa
     1141 aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct acagtttttc
     1201 ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga gaataaacgc
     1261 tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta tctcttttta
     1321 ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg tattgatttt
     1381 tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt taaaaattac
     1441 aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc tgattgttta
     1501 cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc agatgactgg
     1561 gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga aaaaatcagc
     1621 tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat gtttttttca
     1681 aaga
//
LOCUS       DAJSVD010000237         1664 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSVD010000237 DAJSVD010000000
VERSION     DAJSVD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.244"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            131..505
                     /locus_tag="ON498_003000"
     CDS             131..505
                     /locus_tag="ON498_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3941197.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ON498_003001"
     CDS             complement(619..1248)
                     /locus_tag="ON498_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3941198.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ON498_003002"
     tRNA            complement(1382..1454)
                     /locus_tag="ON498_003002"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAJSVD010000238         1575 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSVD010000238 DAJSVD010000000
VERSION     DAJSVD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.245"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="ON498_003003"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="ON498_003003"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="ON498_003004"
     tRNA            203..276
                     /locus_tag="ON498_003004"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="ON498_003005"
     tRNA            316..405
                     /locus_tag="ON498_003005"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="ON498_003006"
     tRNA            420..491
                     /locus_tag="ON498_003006"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="ON498_003007"
     tRNA            507..579
                     /locus_tag="ON498_003007"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="ON498_003008"
     tRNA            589..661
                     /locus_tag="ON498_003008"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="ON498_003009"
     tRNA            697..772
                     /locus_tag="ON498_003009"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="ON498_003010"
     tRNA            774..854
                     /locus_tag="ON498_003010"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="ON498_003011"
     tRNA            861..934
                     /locus_tag="ON498_003011"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="ON498_003012"
     tRNA            937..1009
                     /locus_tag="ON498_003012"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="ON498_003013"
     tRNA            1021..1092
                     /locus_tag="ON498_003013"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="ON498_003014"
     tRNA            1138..1208
                     /locus_tag="ON498_003014"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="ON498_003015"
     tRNA            1229..1313
                     /locus_tag="ON498_003015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="ON498_003016"
     tRNA            1375..1447
                     /locus_tag="ON498_003016"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="ON498_003017"
     tRNA            1457..1528
                     /locus_tag="ON498_003017"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
BASE COUNT          369 a          353 c          414 g          439 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctg
//
LOCUS       DAJSVD010000239         1545 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSVD010000239 DAJSVD010000000
VERSION     DAJSVD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.246"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            239..1408
                     /locus_tag="ON498_003018"
     CDS             239..1408
                     /locus_tag="ON498_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3941199.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          567 a          220 c          249 g          509 t
ORIGIN      
        1 ttttattact atgtttgggt tttatagcaa agaaagttta ataagagttt ggacctagca
       61 aacaatatta atgaggtcat taaatcttat tatctttagt gatataacga aatatctaca
      121 cagacaaaat gatagcgtaa aatattttgt gtaaatagat atttagggta gaaaaaaaga
      181 aagtccattc tgtaaaatta aagttaccac aaccgaaatt ttataggagg actttctcat
      241 gcccaatttt actacagaaa ttatggaaac actaatcaat aaaggtgatt tagatgaatt
      301 atttcgatgt catttagaat tagctgttaa ttcattattg caggctgaat taacagcttt
      361 tcttgattat gaaaaatatg atcgtgctgg gttcaattca ggtaattccc gtaatgggaa
      421 ttattcacgt tcattcagaa cagagtatgg agaattaaat ttggtaattc ctagagatag
      481 aaatggtaag ttttctcaac aaactttacc tgcttataaa agaaccaatg actctttaga
      541 aaccactatt attcagctat ttcaaaaagg aattactatg tctgaaatct ctgaattgat
      601 tgaaaaaatg tacgggcatc actatacgcc acaaaccatt tcaaatatga ctaaaatagt
      661 ttctgaagat attattgcct ttaaagaaag gtccttagaa tccagatact ctgtcatttt
      721 tatggatgct actcacattc ctttaaaaag acaaactgtt tcaaaagaag cagtttatat
      781 tgtaattggc attcgattag atggaaccaa agaggttctt gggtttagtc ttgctccaac
      841 tgaatctgct tatgtttgga aagagattct tcaagaccta aaagaccgtg gtttaaaaga
      901 ggttttatta gtcgtaactg atggtttaag tggcattaac gatagtattc atagtgttta
      961 tccaaatgct caatttcaac aatgttgtgt ccatatatct agaaatatcg ctcataaggt
     1021 tcgtgttagt gatcgacaag aaatctgtag cgatttcaaa ttagtttatc aagcaagttc
     1081 aaaagaagaa gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc
     1141 acgagtggtt aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc
     1201 aatcagaaga actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa
     1261 atataccaaa agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc
     1321 tcaattcaac aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat
     1381 acatgataca ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca
     1441 caaaattatt gacgctcccg acaaaattat gatcttgcgt aaagttataa aatattaata
     1501 aaaatatttt attaatgaaa agcttggtag cacggttatt catat
//
LOCUS       DAJSVD010000240         1530 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSVD010000240 DAJSVD010000000
VERSION     DAJSVD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.247"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            20..1486
                     /locus_tag="ON498_003019"
     CDS             20..1486
                     /locus_tag="ON498_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR3941200.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          421 a          289 c          325 g          495 t
ORIGIN      
        1 aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa
       61 gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg
      121 tgcattactt atctattata tgtacgatac agttgccaac gggggacttg gattaccacg
      181 agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt
      241 cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt
      301 tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt
      361 ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt
      421 cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat
      481 gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata
      541 taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata
      601 catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac
      661 aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc
      721 agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat
      781 cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga
      841 tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat
      901 cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg
      961 tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc
     1021 aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg
     1081 tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt
     1141 gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt
     1201 gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc
     1261 tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt
     1321 agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac
     1381 agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt
     1441 tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa
     1501 aacaggaatt gcttatttgc agcagttcct
//
LOCUS       DAJSVD010000241         1497 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSVD010000241 DAJSVD010000000
VERSION     DAJSVD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.249"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            10..>1497
                     /locus_tag="ON498_003020"
     CDS             10..>1497
                     /locus_tag="ON498_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941201.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLD"
BASE COUNT          604 a          208 c          225 g          460 t
ORIGIN      
        1 gagatttata tgtttatatt agatgatgaa ataaaaaaag ttaaaaatcc tagaattatt
       61 ccattattag aagaagttag aagtagtttt tatcagggaa attatcgttc tgcaatagct
      121 gtcaattaca gtgcgcttat tttagattta attgataaat tatctgattt agcaaatatt
      181 tacgaggata atacttctaa gaaaatttta gaagatatag ctagactaag aaaaagtgat
      241 ccaaactctc caaaatggga aatggaacta ctagaaaaag cttatagttc aactgagcta
      301 attgatagct atgaatatga ggatttaaaa tatattaagg atcaaagaaa ttattcagct
      361 catcctgtag tcacttacaa cggtaatact tgggaattaa gagatataac aaaagaaact
      421 tgtcaggatg ttatacggaa gtcctatgaa attatattcc taaaaaatcc aatattagga
      481 aaaaaagtaa cgatcgaaat tatacaatat gcaagtcgca tacatgctat gtctgttaca
      541 cctgaagaat ttcactctgc actaaaaaat agctatctaa ataaactaag cgaaaaagcc
      601 aaagaaaacc tatgtaagac agcctttaag tttgtttttc aattatctta tggaaatgag
      661 gaagcagata gagataggga aagtactttc agactactta atgccctaat ttttgataat
      721 aaagaattct atttaaatat tctaaaaaaa gaaattgcaa agtataggtt cacagctgaa
      781 tcaagagagg aaattaataa atcacttggt gaaaatcaac agatcacgta tacaaaaggg
      841 ttctttttac ttacttttat agctacccat ccagaattac aacatgattt ttcagacgag
      901 acaattcaaa atttacaaat atcaataaat gattttaaac cgaaacaaca agtactaaca
      961 gaaatagaat attactaccg tttacgttcc cttgcagttc taggaaacaa caatgaagaa
     1021 ttacaaaatc acttaaatag tctagttgat cctatactcc agcaaaaaat gagagtttct
     1081 tcgctaccat cttcaacatt agaaacaata tataatcaat atttatattt tggtgaaaaa
     1141 gagatgttca tagattttat gatcgaacat cttaccgatg ctagttcatt caggttagca
     1201 gataaggata tggaagtatt accatggatt tacaataaat taaatcagga gcaatttaac
     1261 aaattactat acaacctaaa tggaaataat caattttaca aaaacagtaa ttttccgcct
     1321 tttatttcta atctgttaga cttttataat cagaaattcc aagtgaattt acgtaaacat
     1381 tatagtgggc ttctatatcc aaatgcagtg actatggatc agggttttca attaaaggat
     1441 aaagaattaa aaaaactgta tgaattagct gaatcgaaaa gtgatatgta tctagac
//
LOCUS       DAJSVD010000242         1489 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSVD010000242 DAJSVD010000000
VERSION     DAJSVD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.250"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            239..>1489
                     /locus_tag="ON498_003021"
     CDS             239..>1489
                     /locus_tag="ON498_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3941202.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          475 a          284 c          278 g          452 t
ORIGIN      
        1 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
       61 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
      121 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
      181 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
      241 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
      301 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
      361 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
      421 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
      481 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
      541 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
      601 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
      661 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
      721 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
      781 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
      841 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
      901 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
      961 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     1021 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     1081 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     1141 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     1201 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     1261 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     1321 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     1381 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     1441 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DAJSVD010000243         1481 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSVD010000243 DAJSVD010000000
VERSION     DAJSVD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.251"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..534)
                     /locus_tag="ON498_003022"
     CDS             complement(<1..534)
                     /locus_tag="ON498_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3941203.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDD"
     gene            complement(654..1301)
                     /locus_tag="ON498_003023"
     CDS             complement(654..1301)
                     /locus_tag="ON498_003023"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HCR3941204.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
BASE COUNT          483 a          312 c          178 g          508 t
ORIGIN      
        1 atcatctctt gtataaataa ataatggtgt taatacacca atgaccaaac ttgctaaagt
       61 agctccaaca tttcggaatg tcgatagcat tgttctttct tctgtaatag aagtaatagc
      121 tgatgccatt gatccataag gaatatttat agatgtgtaa aaaatactcc cccataatat
      181 atacgtaaca tacatgtata ctaccttaac gctcatagaa gctgaaatca tagaagtttg
      241 atacattaaa aaacttgcta aagcgactgg accactcatt cttaaaatcc acggtttaaa
      301 cttaccctgc ttagttgctt tcgatctatc aacaatacgc cccatagtta catcagtaac
      361 tgcatcgata aatcttgcta ctaaaaataa tgttccaacg gcagctccgc ttatccccaa
      421 tacttttgta taaaagatca ttaaaaaaga actagcaaaa atgaaagtaa aatcgtttcc
      481 aaaatctcca aacatatatc ctattttgtc tcttatacca aattttcttt ccatacaatt
      541 ctctcccttc tgttgttaag cgttttcatt ctgatgaaag tacgtactat actctgaagc
      601 gtaaagagtc agtatttatt ttttacattt cagagtatta tttgtttttt ctcctaataa
      661 ttctttatct cataaaactt atctatatat ttactagcta ctcgacttac ttcttcaaaa
      721 tcaccttttt caacagaagc caataaattc ccacctacac caactgcaac aactcctact
      781 tcaaaccaat cttctaagtt ctctatactt actccgccag taggcattat atttacgtgt
      841 ggtaatggag cttgataggc ttttacaatg gatggaccaa aagcagaacc tgggaatagt
      901 ttaatgatat ctacgccagc agtaagtgcc tcctttatct cagtaattgt catacaacct
      961 ggtaaataag gaatttgata taaattacaa atctcagctg tttgtcgatc aaaagatgga
     1021 ctaacgatat attctgcccc agccataatt gcgatacgag cagtaaacgc atctaaaacc
     1081 gtaccggctc cgattattac atctttttga tcagcatatt ttttagcaat ttctttgatt
     1141 atttcttcag cattaggaac tgtaaaagtt agttcaatcc ctttaattcc acccgcaata
     1201 attgaattta ttgctttaat ggcctcatcc ttatttttcc cacgtacaac cgcaatgact
     1261 ccagccgctt ctaacttact taaaatttct acttttttca tatctatctc cttcctttat
     1321 ggatacatac tttgtctagc tgtactaact tacaaagaat aaatttaagc ttttatttca
     1381 agtattattt tctacttccg atttcaaaaa aattaaaata attatacgca ttacgataac
     1441 aaatattctc cactatctga ctaagataca tttcatcatc t
//
LOCUS       DAJSVD010000244         1463 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSVD010000244 DAJSVD010000000
VERSION     DAJSVD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.253"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..424
                     /locus_tag="ON498_003024"
     CDS             <1..424
                     /locus_tag="ON498_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3941205.1"
                     /translation="LDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMV
                     KRIENEVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDY
                     LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            429..1376
                     /locus_tag="ON498_003025"
     CDS             429..1376
                     /locus_tag="ON498_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR3941206.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          490 a          208 c          292 g          473 t
ORIGIN      
        1 tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa tagatgaaca
       61 tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac gtaatcccta
      121 ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat caagatacac
      181 atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc
      241 tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag
      301 agattatcta attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt
      361 tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag
      421 gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag
      481 ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag
      541 tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat
      601 ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag
      661 ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa
      721 gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt
      781 ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg
      841 tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag
      901 attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc
      961 caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata
     1021 agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta
     1081 gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag
     1141 ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga
     1201 ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt
     1261 cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac
     1321 cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca
     1381 tactaagata agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt
     1441 gtaaaatcaa atctgggata gaa
//
LOCUS       DAJSVD010000245         1461 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSVD010000245 DAJSVD010000000
VERSION     DAJSVD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.254"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..513)
                     /locus_tag="ON498_003026"
     CDS             complement(<1..513)
                     /locus_tag="ON498_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941207.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKI"
     gene            618..863
                     /locus_tag="ON498_003027"
     CDS             618..863
                     /locus_tag="ON498_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941208.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1019..>1461
                     /locus_tag="ON498_003028"
     CDS             1019..>1461
                     /locus_tag="ON498_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HCR3941209.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTS"
BASE COUNT          509 a          210 c          249 g          493 t
ORIGIN      
        1 tatttttttc aacttcatat tgtacatatc ttcagccata aaagttgagt tcgtgatttc
       61 atcttgggtc tcattaaaaa agttatccga ataatcgctt atttttgaaa agtctttatt
      121 ggcaccagcc tcaaaaaatt gttcaacttt ttcagtataa agttgtgcta acgctccttt
      181 ttcaatatta actttaatat tttcatgtct atcaatttgt gtgctgttca gtaagggaag
      241 atcagctttt agttttatat cgtcttgatt tctttcaatt tcaattggca aaatcttttc
      301 agtagattca gtttctgata aaggaacatc tagtttttta tcatgataat aagcttcaaa
      361 tccatctgga accggaaaaa aatgtattgg gtaattcttt tctgaggttg ttgtactagt
      421 ttccgtactt gtagaacttg tgtcagaagt ggtacacccg cttatagttc cgatgagtaa
      481 agcgcaagtt agaatgattg caaatttctt caaaaaaatt cctccttaaa attgaatatg
      541 gactaatact agtcccattg tagcataccg aatctttaag attaataaga aaaaggacta
      601 attttagtcc aaaaggtgtg ctattcttga aagaggaaaa aattattgat aaagaaaatg
      661 taatcggtag caatattaga cgagtccgat tagaaaaagg aattggtcaa accgagctga
      721 ttagagacct acagcttcgt aaaattgcta taactaggga aacgttggtt aagattgaag
      781 gtggccgcca acacattaag ttggatcaat tgaaagccat caaagatatt ctacaggtgt
      841 cttatgaaga tttgcttcaa tgactacttg tagtcatttc gtttgaaatt attatgttac
      901 acccttatgt gtcacgaaga aatactggta taataatgaa tgggatatgt aacgataggg
      961 tatactgtta tgttgcctaa taaattgtgg atcaaaatac aagaatggag ggatatcctt
     1021 gtataaggca tatgaattgg attttacaaa tgctgactta aatgcattct cctcaagctc
     1081 gcaatcatat acacatgtag taaatcaaat agatcacaac caaaaatcta ttaacaagag
     1141 aatagaaaat ctatttgaaa gtgaaaatga tttagttgat tattattcta aggacagcaa
     1201 aattctagat gcagacgaaa ttattaaaga ttggtttcca actataaaag cagatatttt
     1261 tatatctcac tctcatgctg atgaaaaatt agctatacgt tttgcatcat ggctatttga
     1321 aaattttggt cttactgcat ttattgattc ttcggtttgg ggatacagtt ctgatttatt
     1381 aaaaaaaata gaccaaaaat attgctataa ggaacaaaca aaaacatatg attatgataa
     1441 aagaaatgta acaactagtc a
//
LOCUS       DAJSVD010000246         1417 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSVD010000246 DAJSVD010000000
VERSION     DAJSVD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.255"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            60..1004
                     /locus_tag="ON498_003029"
     CDS             60..1004
                     /locus_tag="ON498_003029"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3941210.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          508 a          210 c          282 g          417 t
ORIGIN      
        1 aaacaagact atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca
       61 tgaaaaaaac aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg
      121 cttatgcaat gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag
      181 aaaaagcaga aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg
      241 tggctgtatg gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag
      301 ctggtatcaa ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa
      361 tcatgcgcca aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg
      421 cttcaaaccc agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat
      481 cacgtgttat cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat
      541 tgaaattaaa agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt
      601 cagaagttgc tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag
      661 aaaaagacca tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg
      721 ctgcttatga gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg
      781 cacgtatcgt aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt
      841 taactggtga atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa
      901 atggtgttcg tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca
      961 aatcaacaag tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga
     1021 actaaaaatc ctaaaactgc ttaacagctg aaatagctgt taagcagttt tttattgata
     1081 tctaataggt aagcaaataa acagaatggt taaaattaat cggtccgaaa atgtatccgt
     1141 acatatgaac atatgaaatt taatctgttg attacaataa gcgaatcgtt atcaatgttt
     1201 atctgtggtt aattaaaaaa tccttccgca acggcaaata caatgattca tatctaattc
     1261 gttcatattt gattaactga ataaagaaac tggtttttat cattcaatta aacgacaaaa
     1321 aaagtaagaa aaactgcaaa atttttttat aattttgcat aagcagattt tgatggaaaa
     1381 atgacgattt ttgataaaaa accgttcgag agaggtt
//
LOCUS       DAJSVD010000247         1416 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSVD010000247 DAJSVD010000000
VERSION     DAJSVD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.256"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            31..441
                     /gene="spx"
                     /locus_tag="ON498_003030"
     CDS             31..441
                     /gene="spx"
                     /locus_tag="ON498_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3941211.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            455..811
                     /locus_tag="ON498_003031"
     CDS             455..811
                     /locus_tag="ON498_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3941212.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          521 a          209 c          265 g          421 t
ORIGIN      
        1 attggattat aattaaaaag gagtgagaaa atgataaaag tatatacttc gcctagctgt
       61 acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt tgaagagaag
      121 aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc agcgactgaa
      181 ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct gggcatagat
      241 tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc ttcattattg
      301 agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga agatgagatc
      361 cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta tttacaaaac
      421 aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct atggatcagc
      481 gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat tgccttatca
      541 atttgttgac ttagaagaac gcgatctaag tacagaagaa gcagaagaat tatgcagttt
      601 tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc acaacattgc
      661 gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac aacataccaa
      721 tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat acaatgagca
      781 gttgatggaa cagcttatct taaataaata gactttaact gttttaatca agtcactgag
      841 catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt gttctttttt
      901 tgactttatt tttctactat agagacatgc tcaatttatg tggtcaaaat tcaaatctag
      961 gtggggatat agagttaaaa atatattgac atgtgtaaat ttacatgata tattcatttc
     1021 tgttgtttta agtagcaaag caataaaata gtattttaaa aaacatataa aatagttgtt
     1081 gacatgttag aaatgacatg ataagataac tgagttgtta aaaaagaacc acttgtgaag
     1141 cttgattcta tcgaaaaaaa cttcaaaaaa gttcttgaca aataacaaat gataagttat
     1201 gataaatgag ttgctgtaag atttcgaaaa acgaaatcaa aaaaactttt aaaaaagagt
     1261 tgacaattac ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
     1321 ggggaacact cctgatgaag tagacctttg aaaactgaac aaagtaagac aaaccaaatg
     1381 tgtagggcgt cttgattcaa ttcaagacaa caaaca
//
LOCUS       DAJSVD010000248         1414 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSVD010000248 DAJSVD010000000
VERSION     DAJSVD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.257"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..305
                     /locus_tag="ON498_003032"
     CDS             <1..305
                     /locus_tag="ON498_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR3941213.1"
                     /translation="NENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTD
                     GLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            550..>1414
                     /locus_tag="ON498_003033"
     CDS             550..>1414
                     /locus_tag="ON498_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR3941214.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          553 a          169 c          282 g          410 t
ORIGIN      
        1 ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga aaacaagtct ttaaagtcta
       61 aaataagcga tttaaagcgt gatgtgagtt taatctatga aagcactaag gaattcctta
      121 aggaacgtac agacggctta aaagccttta aaaacgtttt taaggggttt gtagacaagg
      181 taaaggataa aacagcacaa ttccaagaaa aacacgattt agaacctaaa aagaacgaat
      241 ttgaactaac tcataaccga gaggtaaaaa aagaacgaag tcgagatcag ggaatgagtt
      301 tataaaataa aaaaagcacc tgaaaaggtg tctttttttg atggttttga acttgttctt
      361 tcttatcttg atacatatag aaataacgtc atttttattt tagttgctga aaggtgcgtt
      421 gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa
      481 aaaccccatc tgttaaagtt ataagtgacc aaacaaataa ctaaatagat gggggtttct
      541 tttaatatta tgtgtcctaa tagtagcatt tattcagatg aaaaatcaag ggttttagtg
      601 gacaagacaa aaagtggaaa agtgagacca tggagagaaa agaaaatcgc taatgttgat
      661 tactttgaac ttctgcatat tcttgaattt aaaaaggctg aaagagtaaa agattgtgct
      721 gaaatattag agtataaaca aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg
      781 ttttgtaaat ccaggctttg tccaatgtgc aactggagga gagcaatgaa acatggcatt
      841 cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc caacagttcg ttggttgttt
      901 ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat taaataagag tttgtcagat
      961 atggctcaag gatttcgccg aatgatgcaa tataaaaaaa ttaataaaaa tcttgttggt
     1021 tttatgcgtg caacggaagt gacaataaat aataaagata attcttataa tcagcacatg
     1081 catgtattgg tatgtgtgga accaacttat tttaagaata cagaaaacta cgtgaatcaa
     1141 aaacaatgga ttcaattttg gaaaaaggca atgaaattag actatgatcc aaatgtaaaa
     1201 gttcaaatga ttcgaccgaa aaataaatat aaatcggata tacaatcggc aattgacgaa
     1261 actgcaaaat atcctgtaaa ggatacggat tttatgaccg atgatgaaga aaagaatttg
     1321 aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa ggttaatctc ctatggtggt
     1381 ttgttaaaag aaatacataa aaaattaaac cttg
//
LOCUS       DAJSVD010000249         1403 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSVD010000249 DAJSVD010000000
VERSION     DAJSVD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.258"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(27..>1403)
                     /locus_tag="ON498_003034"
     CDS             complement(27..>1403)
                     /locus_tag="ON498_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3941215.1"
                     /translation="RTIKELIRKINVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLA
                     VFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLK
                     KFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQI
                     EKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSR
                     PCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQF
                     PLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREK
                     IDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLF
                     RKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKG
                     YENKTKRM"
BASE COUNT          448 a          194 c          251 g          510 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccg
//
LOCUS       DAJSVD010000250         1392 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSVD010000250 DAJSVD010000000
VERSION     DAJSVD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.259"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            106..>1392
                     /locus_tag="ON498_003035"
     CDS             106..>1392
                     /locus_tag="ON498_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR3941216.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          472 a          270 c          259 g          391 t
ORIGIN      
        1 aattgattct tcagccccat tttcatttta ggtattaaaa aacatatcgt tctctagtat
       61 gattaaatca ccacaaaata aacaactgag aggacgatac gctatatgaa tgattctatc
      121 aaaaaaatgc tgagaataac agagaaagat ttgatgatta cagaggtctc ttacgagacc
      181 cttcagaaga aaaagacgtt ggtcgtcgat gctgttctct cgcctactcc tcgtgcttgt
      241 agaagttgtg gttctactgt ggtagatgga aacgggaaag caattatagt gaaaaatgga
      301 aaaaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tatgcgccta
      361 aaaaagcaac gctatacctg taaaaactgt cgcacccatt ggacggctca aagttatttt
      421 gtccaaccca gacattcaat cgcaaatcat gttagatata aaattgcttc tttactgact
      481 gaaaaagtat ctttatcttt tattgcgaaa agctgtcagg tatctttgac caccgttatt
      541 cgtacattga aagagtttaa aagctattta ccaaagcaat ctaagaagat cctccccaga
      601 gtattgatgg ttgacgaatt tcgttcgcat gcttccatag aagataagat gagctttatt
      661 tgcgcggatg gcgaaacagg aaaattaata gatgttttgc ctacgcgtaa attacctcga
      721 ttaacaagct atttcttagg ttgtaccaat ccagaagaag tagaattctt ggtgacagac
      781 atgaacgccg cctacttcca gctcaccaaa cgtgttctgc caaatgcgaa agtcgtgatt
      841 gatcggtttc atattgtcaa acacatgaat caagcgttca atgagttgcg tatccgtgaa
      901 atgaatgaac ttcgtaaagc cggacagaaa agccaggcag aaaaactgaa aaagaactgg
      961 cgctttttgc taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt
     1021 ttccgagcac caaaataccc atttcttact gaagcaatga tgattgatcg attgcttgag
     1081 ttttctccgc ccctaaagga ggcgtatccc ttttttcatg aattagttga agcctttcga
     1141 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
     1201 agctttcggg aaaagcttca aaaccttctg acctatgaag aaggcatcac caacgcaatg
     1261 atctatcctt attccaatgg aaaaatagaa gcgaagaata cccacataaa gacaatgaaa
     1321 cgagtatcct acggatttaa atcatttgag aacatgagaa ttagaatctt tttgatcaat
     1381 caattaatca at
//
LOCUS       DAJSVD010000251         1374 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSVD010000251 DAJSVD010000000
VERSION     DAJSVD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.260"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            33..1372
                     /locus_tag="ON498_003036"
     CDS             join(33..474,474..1372)
                     /locus_tag="ON498_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR3941217.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYRVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          577 a          177 c          239 g          381 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattacaggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAJSVD010000252         1370 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSVD010000252 DAJSVD010000000
VERSION     DAJSVD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.261"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..1095)
                     /locus_tag="ON498_003037"
     rRNA            complement(<1..1095)
                     /locus_tag="ON498_003037"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          309 a          376 c          257 g          428 t
ORIGIN      
        1 cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg ggaccttagc
       61 tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt ctgactcccg
      121 gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat gggcccctag
      181 tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa agctatttcg
      241 gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca cctcatcccc
      301 gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg
      361 acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc ctattcagac
      421 tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg
      481 ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt gtaggcacac
      541 ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc tcacggtact
      601 ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc cggattccga
      661 cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca catttcgtct
      721 acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct gaaagaacta
      781 ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt ttcccgtttc
      841 gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact tagatgtttc
      901 agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca tcctattaaa
      961 gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag ctccccaaag
     1021 catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc
     1081 cttattcact taaccttatc aaccttgcgg ttgggttttg atccttcttc tagcgataga
     1141 agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag ctcacaaatc
     1201 agcctgtgcg gcaaaaagat cgatcgtctc ataaaatcaa agattttata gccgtccgcc
     1261 tattttgctt taggctaaac aatttgttca gctttcaaat tcaacgcggt gttctcggtt
     1321 tgtattactt acatttactt caaatatcca gttttcaatg aacaaattta
//
LOCUS       DAJSVD010000253         1364 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSVD010000253 DAJSVD010000000
VERSION     DAJSVD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.262"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(185..799)
                     /locus_tag="ON498_003038"
     CDS             complement(185..799)
                     /locus_tag="ON498_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3941218.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          460 a          262 c          224 g          418 t
ORIGIN      
        1 acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg atccatctta
       61 aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg ttttcttttt
      121 ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt caacatgtgg
      181 ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac aaaacttgct
      241 gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct agatttggca
      301 aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa
      361 ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca aatccagtaa
      421 caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg attgccgggt
      481 ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa agctgatcaa
      541 taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga ttttcaatca
      601 atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca ccacttagtg
      661 tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg atccaagtgc
      721 cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg tgataatagt
      781 caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg aatagaaaca
      841 gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca catgtggtct
      901 ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt gacatagcaa
      961 ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt caccctagat
     1021 taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat cggtcagggt
     1081 gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca gggagccttt
     1141 ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca agtgcaagca
     1201 agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata aaaaaaggaa
     1261 tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat aagtgaaaaa
     1321 agcttttttc caccaattaa ataaatctct catctcaata gttt
//
LOCUS       DAJSVD010000254         1364 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSVD010000254 DAJSVD010000000
VERSION     DAJSVD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.263"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(140..1288)
                     /locus_tag="ON498_003039"
     CDS             complement(140..1288)
                     /locus_tag="ON498_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3941219.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          241 c          216 g          514 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaattg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAJSVD010000255         1347 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSVD010000255 DAJSVD010000000
VERSION     DAJSVD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.265"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(141..1052)
                     /locus_tag="ON498_003040"
     CDS             complement(141..1052)
                     /locus_tag="ON498_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3941220.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT          365 a          254 c          201 g          527 t
ORIGIN      
        1 gattctaagt aaaaaaaatt tttaaaataa aaagaactaa atcaagtagt aattaaacta
       61 ctgttgattt agttctaata aaaagattta gatgaacagt tttattgatt taaatagttc
      121 atatttttta tttataccgc ttatttacta cttgctgtac tagaatctga agtgaatgta
      181 ctcaaaacat tttcaaagtc tttgtctttg atttttacat tagcgtcttt caatacttcg
      241 ccaattactt tattctggaa ggtactgtca cttaattttg tgtctgttgc aatttctttc
      301 aattcttttt tgtatttatc catgtcgttg cctttgtttt ggtttttcac cattttaacc
      361 acataatatt ccgtagtata agttgaagca ttggtagaag tgatcacatc agaaacttga
      421 ccatctttca acttaaaggc tgcttctttg acttctgccg gaaccgttgt agtagtggaa
      481 tcaaatttca cttttccgcc atcttctttt gtcgtagtat cagttgactt gtctttggct
      541 aatttagaga aatcgtctcc cttatcagca gctttcttca catcttttgc atcatcttca
      601 cttgctactt ggataatttg agcttccact tctggatgga aagattccca agccgttttt
      661 aaatcttcat cggtcaattt gatatgtttc tttagacctt cttgaaaggc tagactttgt
      721 ttgatactgt ctttgaatgt ttgttctgtg taacctgcag atttcaattg tgaatcaaat
      781 gaatcaccca attgtttctt tgtttgatca tattgagaat cgatttcttt ggttgatacg
      841 tcatctccat atttatcttc aaatacttga taaacgatca actgttgcac agcggaatct
      901 gctccttgca tttttttcaa gctatccatt acttcttgtt cgctgattgt accgccttta
      961 taactaacgg ctgttttttc accggcattc gtacagcctg acaataagcc agtgcctagt
     1021 acgatggcaa gtgctaaaaa tctttttttc atttatcaat tctcctactt ttcgtttttt
     1081 gcgaattgct aaaaacaaag gtgttcaaga tgaaaatcat caagaacacc tttactaatc
     1141 tgctcgatac gtcctgaata gatataaggc tacacaatcc agaaagttcg ctcagcagaa
     1201 taaatgtcaa gtagcacatc acaccttaat atactacttc ttttgttacc gtttttcaag
     1261 tctatcttgc gttccgtatt taacgtaact aattcttaga acaaatgtta tcgattttgt
     1321 ttttcttctt ccgctgctgg gatattc
//
LOCUS       DAJSVD010000256         1330 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSVD010000256 DAJSVD010000000
VERSION     DAJSVD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.266"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(435..926)
                     /locus_tag="ON498_003041"
     CDS             complement(435..926)
                     /locus_tag="ON498_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HCR3941221.1"
                     /translation="MPDLDFTTNTHLHFLEAYINFYMTTPTFDVKTTLQRLLIVFTDK
                     IATPANCLQFFNADWCPLSQEISFSHDIETIWVLQRTLTVTGIKDVQVSQFIENLGTQ
                     VATKGFDNNKIICDHSNTLQTSTRKTWWTLAEAIIGFYNLYQLTNSKVYYELATHHFE
                     MVE"
BASE COUNT          412 a          223 c          291 g          404 t
ORIGIN      
        1 aaataataaa ttctgatatt gaaataaaga tagaaaatta aaagggatta cctttatttt
       61 aattcaaaaa ttatgaaaaa tagggtggag ttgctggaga gaatcaatta ggttgtagta
      121 ttaaggataa ccaagagcta aagaaccatt tgcgcatata tttgcatcta agcagaaatt
      181 actaaaataa actatcaaaa agccaactgt tctttagttg gctttttgcg atgtcactac
      241 aattcgaatg attctctaga agtcaggtcg tcctgttttt cttacctaga taggcgtgca
      301 attgtttctg tgtacatacg acaggtatgg taaggggcct ttccaagcgt tggcgatatc
      361 ttctgtagaa tctggtgtcc atcattgatg aggtaggcat accattcact gttagatcga
      421 gtatccacaa agtttcattc taccatttcg aagtggtgag tagccaattc ataatagacc
      481 ttactattag ttaattgata caggttgtaa aagccgatga ttgcttctgc tagcgtccac
      541 caagtttttc gagtcgaggt ttgaagggta tttgaatgat cacaaatgat tttgttgtta
      601 tcgaagcctt tggtagctac ttgagtgcca agattttcaa taaattgact gacttgcaca
      661 tctttgatac cagtaacagt caaagtccgt tgcaataccc aaatggtttc aatgtcatgg
      721 ctaaaactga tttcttggct tagaggacac caatcagcat tgaaaaattg gaggcagttt
      781 gctggggtcg caatcttatc tgtaaagaca atgagtaatc tttgcaaggt agtttttaca
      841 tcgaatgttg gcgtcgtcat gtagaaattt atgtatgcct ctaaaaagtg caaatgggta
      901 ttagtggtaa agtctagatc tggcaaatcg ctgggtccga ttgattggga gggaacgggc
      961 taccagttgt gggaaaaatc ctcctgataa ccatttgtaa gagggtcata agacttcttt
     1021 tcgagaaaac taaagagcgt ggttgccatt tttaatgcag acagatcatg aaagtctaag
     1081 taatactcgc taagaacata gagggcaaaa ctattagcat aagtggtttt attgggatca
     1141 gagagtattt cagttgcggt tagctcccag aagatcccct gggttttcct ggtcccaaca
     1201 gacttaccgg aaaaattcat aggcaggatt cgcagcagtc tttaggctag ctaaaaaaga
     1261 ctcagttaag gatttgtctt gcaaaatgcg gcagcaatga ctaaaactcc ataaataccg
     1321 ggcgttcatc
//
LOCUS       DAJSVD010000257         1298 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSVD010000257 DAJSVD010000000
VERSION     DAJSVD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.268"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            19..312
                     /locus_tag="ON498_003042"
     CDS             19..312
                     /locus_tag="ON498_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941222.1"
                     /translation="MGILQRSEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDA
                     TATTALTFGFTKNGTINSDKTRQAATESDGICKKVCNGEENYWIGEEKLNEIY"
     gene            299..751
                     /locus_tag="ON498_003043"
     CDS             299..751
                     /locus_tag="ON498_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941223.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            744..1142
                     /locus_tag="ON498_003044"
     CDS             744..1142
                     /locus_tag="ON498_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941224.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          503 a          157 c          232 g          406 t
ORIGIN      
        1 ctaagcttaa gaaatcggat gggaatactc caaagatcgg aaatagtaaa agaacttttt
       61 tatcataaat ttagttatga aataagacta gatgatactt atgagcatta tagaattgtt
      121 tttttcgtat acaattctat agaagatgct actgcgacta ctgcattaac attcggtttt
      181 acgaaaaatg gaacaatcaa ttcggataaa acccgtcaag ctgctacaga aagtgatggt
      241 atatgtaaga aagtatgcaa tggggaagag aattactgga taggggagga aaagttgaat
      301 gaaatctatt aatgatttat taaaaaaaca tcctgattta tataaaaaat taaattctga
      361 acaaagaaac ctgtccaaac aattacttaa aataagatta aaaaaacaaa aaaacgtgac
      421 agaattctca aaaatgttag gaataagtga aaaaaaatat cttgaatttg agttcggaga
      481 tatgcatatt cctgttgcag agtacaataa acttttgaaa caagttgaaa atttgtccta
      541 taaagattca atgaatttca ctattaaggt tgctggcggc cctattaaat atgaccgtat
      601 tgaaaataaa ccttatattc ggaataaaag ttactcagta tttaaaagta gaaagagaaa
      661 agaagtatcg tcaaatatgg tctatgctag tgttaattac caagatacga gccgctatat
      721 tgtagaaaaa gaggtatatg tacatggata aaggaaatag tgttactttt gcacttgaaa
      781 atattagttt gaataaaatt cattttgaat taactgataa ttttagttat ggagatttag
      841 agtttaatat tgaacttgga tataaagaag acgaagcaga gtatctagaa aaaaaacttg
      901 tttctttacg agctcacatt gtagggggga tggaaggagc accggtagat attttactct
      961 tttcagttat tcgtgtttct acaaatgaac agccatataa aaaattagta gaagaatcta
     1021 tgggagagct agctaaacct atcctgaata aagcttctaa tttgatttct aatttatata
     1081 gcgaagtagg agttatgcct acagttattg atttatatca gctatatagt caacaaaaat
     1141 aaaatacaag taatagagtt atctatttaa atttgcaaga caaagacctc aaccagtcat
     1201 ccttggttga ggcttttttg ctctgtaatt aactcgtata gatcctgcaa gatagtttgt
     1261 ttctgaataa atgattgata tgaagtattt agatgact
//
LOCUS       DAJSVD010000258         1292 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSVD010000258 DAJSVD010000000
VERSION     DAJSVD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.269"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            284..523
                     /locus_tag="ON498_003045"
     CDS             284..523
                     /locus_tag="ON498_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941225.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(885..1145)
                     /locus_tag="ON498_003046"
     CDS             complement(885..1145)
                     /locus_tag="ON498_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941226.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          427 a          236 c          176 g          453 t
ORIGIN      
        1 gctatcctcc tagcaactat ttaaataccc aaaaaaatta cctctagata atatcatacc
       61 tacgttttca cttataaatt ttttcataaa aaaagcgttt agaggcataa tcgttaatac
      121 agatggtaac caatgtatct ggtttgaact gggatatcac cctctccata tatgtattac
      181 taagactaat agatactata aaagacttga tagttttgca ttgattttgc ctaatactga
      241 gcataggtgt atgctaaacg atactataag gagtgtgtac ataatgaaaa gaattctgtt
      301 tatcagttta tcaaatttat tgttacttac tgcttgtggg acttctgcat caaagaacat
      361 accttcagat acgcaaattt cgaatagtat cgattcaaca atttctacaa atgagaaaaa
      421 ttctccggta gaaacttccg atataaaagt ttctacctct gcaaaaaaca gggtaaagaa
      481 actgtcagca cgtctccttt atttcaaaca aactctgaat tagcttcttc atcttcttca
      541 gcaaataatg atgatagttt cgcgtttact cctaactaaa tgcaaccttt acaaatgctt
      601 tacgataaca agcctgagct aaatgatcct gatatcagtt ttgcattttt tgaaatgatt
      661 ggatcggatg atctttttaa aactactagt ttgtctatcc gaaaatagga cagttctggc
      721 agtataggtt tttatcgagt ttctccacca ggcctcgtta cagagacaga ttctctaggc
      781 gaaccttttg aataaatcaa aaatcatgaa caatcactgc caactaaata aagtgccttc
      841 aaaagcttgg tcttctcgct tttgaaggca ctttatttaa atctttatga aactaaagta
      901 tgttcatatt tgttgctata gttatataga tattctgttg tttccaactc ataaacgtga
      961 gtatattttg ccactacttt tccgcatttt tctaatattt cattgcgatc gacgtcatga
     1021 tattcgtcta cgcagatcac aacacaattt tctaatttac tgattacttc tccaaccact
     1081 ttttgtttca agccaatagg ttggcaaata tatttttttc ctaaaatgat agtttcgtta
     1141 ttcatatttc atccatcctt aatgtttttc cattatttaa aatgataata ccattaaaat
     1201 atgattatga aaatcaataa cacttgtgat tttataaaaa aataaaaaaa ccgaaaaaca
     1261 gaaatgccca ctgtctttcc ggctcttttt at
//
LOCUS       DAJSVD010000259         1282 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSVD010000259 DAJSVD010000000
VERSION     DAJSVD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.270"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(112..597)
                     /locus_tag="ON498_003047"
     CDS             complement(112..597)
                     /locus_tag="ON498_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HCR3941227.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(599..>1282)
                     /locus_tag="ON498_003048"
     CDS             complement(599..>1282)
                     /locus_tag="ON498_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941228.1"
                     /translation="QINDLSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAV
                     DYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQ
                     DVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQ
                     NENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIV
                     SLINSIQED"
BASE COUNT          412 a          263 c          135 g          472 t
ORIGIN      
        1 cctaattcag tttgatgaat taactgataa aaaaatatct actatttaaa gatacaggga
       61 taagtaagta aaaaaataac atgtcattag atagtagaca gttccaactc tttatttacc
      121 ttttttgtat gctgcactgt caatccaagt tcctaaattg ttgtatccat catcacttat
      181 ataatcatat tggccatcaa taatttcgtc aaaccacaaa tattctccat ctggtttttg
      241 cccaattatt ttaattactg attgactagt agttgtcatc tggttaaaat cttttatttt
      301 acctatatta acggccaaaa tagcatttcc tcgcttataa ctctcctgga tttcatattt
      361 aacatattct cgatcaagag tttctttacc tatcagcacc acagtcactg atgttccaat
      421 taattgatta tctatccagt tttttatagc aatatctcca ttacgtttaa ctttctcaaa
      481 ttcagcctta tctacaaaac cagcttcctt tgttcctcgc aaagtccagc tgtttctaac
      541 taccattgct cttgttacat ctgcataatg gaaactaaaa aaaacccttc tcaccatatt
      601 aatcctcctg aatactattt attaaactga caatttgttc aactttttta tctacttctc
      661 cttcagtatc taatttttct agcgcttgaa atttactttt taaggaatca tcaaaataat
      721 cactgaaatt ctcttgaatc ttatcaaaca aaaccttact tgcacctttg gtaaatccaa
      781 tcggaactac tatattttca ttttgaacag ataattcaaa ttcttcaatc attccatctg
      841 ctgtaactat ttcacctgat tcaagtttgt ttccaaaaac aaaaatacta atcccagact
      901 ctgcaataat gttttctcga tatcttctcc actcatgagg catatcaagg ggaagtggtc
      961 ttaaagaaag tacgtcttgt atgttatttt ttcttaaatt tcttctctgc tttagaactc
     1021 ctgaaacaat actattacca attccaagtc cgtaacctga tactattcta taaccggatt
     1081 cagaaagcct atatgctaat ttctcgacaa attttaatcc tttcgattca gatgtaaatg
     1141 gagtataatc aacagcactt cccgaaataa aaatagtttt tctcaaatac ttttttctaa
     1201 tacttttcaa aatatcagta atctcagaat aatcatctac ttcaacaacg tttatcgaaa
     1261 atctggacaa atcattgatt tg
//
LOCUS       DAJSVD010000260         1263 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSVD010000260 DAJSVD010000000
VERSION     DAJSVD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.271"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..983
                     /locus_tag="ON498_003049"
     CDS             <1..983
                     /locus_tag="ON498_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3941229.1"
                     /translation="RTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLETTIIQL
                     FQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVIFMDAT
                     HIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRGLEEVL
                     LVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLVYQANS
                     KEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEGFNKQL
                     KRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          440 a          213 c          208 g          402 t
ORIGIN      
        1 cacgtacatt taaaacagaa tatggcgagt tgaatttaac tattcctaga gatcggaatg
       61 gcgaattttc tcaacaaaca ctaccagcat ataagcgaac caatgattca ttagaaacga
      121 ccattatcca attatttcaa aaaggcatta ctatggctga aatctccaaa ttaattgaaa
      181 aaatgtatgg tcaccactat acgccacaaa caatctccaa catgagtaaa ttggtggctg
      241 aagatgtttt agcttttaaa gaaagaacgt tagaagccaa ttattccgtt atatttatgg
      301 atgcgacgca tatccctgtg aagcgacaga ctgtttcgaa ggaagcagtg tatattacga
      361 ttggtattcg tttggatgga accaaagaag ttcttgggtt tactattgcc ccaactgagt
      421 ctgcttatat ttggaaagaa gttcttcaag atcttagaaa acgtgggtta gaagaagttt
      481 tattagtagt gacagacgga ttaagctgta ttgaagaaag tatccatagt gtgtatccga
      541 atgcccaatt tcaacaatgt tgtgtgcatg tatctagaaa tatcgctcat aaagttcgtg
      601 ttcgaggtcg aaaagaaatt tgtgaggatt tcaaattggt ttaccaagcg aattcaaaag
      661 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
      721 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      781 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      841 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      901 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      961 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
     1021 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
     1081 ttttattaaa ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa
     1141 aagactacct cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt
     1201 cttgtatcaa ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata
     1261 ata
//
LOCUS       DAJSVD010000261         1250 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSVD010000261 DAJSVD010000000
VERSION     DAJSVD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.272"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(61..279)
                     /locus_tag="ON498_003050"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="ON498_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="ON498_003051"
     CDS             complement(545..739)
                     /locus_tag="ON498_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941230.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="ON498_003052"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ON498_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          444 a          212 c          170 g          424 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac
//
LOCUS       DAJSVD010000262         1249 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJSVD010000262 DAJSVD010000000
VERSION     DAJSVD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.273"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1249
                     /locus_tag="ON498_003053"
     rRNA            <1..>1249
                     /locus_tag="ON498_003053"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          327 a          282 c          384 g          256 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccc
//
LOCUS       DAJSVD010000263         1242 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSVD010000263 DAJSVD010000000
VERSION     DAJSVD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.274"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            10..483
                     /locus_tag="ON498_003054"
     CDS             10..483
                     /locus_tag="ON498_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR3941231.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            492..719
                     /locus_tag="ON498_003055"
     CDS             492..719
                     /locus_tag="ON498_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941232.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          390 a          196 c          208 g          448 t
ORIGIN      
        1 gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc
       61 gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc
      121 aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg
      181 atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt
      241 tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc
      301 gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt
      361 ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc
      421 gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg
      481 tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga
      541 gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa
      601 atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt
      661 gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa
      721 ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta
      781 gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata
      841 aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt
      901 tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt
      961 attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg
     1021 ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact
     1081 cttactccat tcttttttag taagctccca tatatactgt aaagccttct atatattatg
     1141 tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat
     1201 ggacaattct tttttatttt taacattatt ttgataataa tg
//
LOCUS       DAJSVD010000264         1231 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSVD010000264 DAJSVD010000000
VERSION     DAJSVD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.275"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            517..903
                     /locus_tag="ON498_003056"
     CDS             517..903
                     /locus_tag="ON498_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3941233.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            997..1188
                     /locus_tag="ON498_003057"
     CDS             997..1188
                     /locus_tag="ON498_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3941234.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          462 a          179 c          188 g          402 t
ORIGIN      
        1 atgatgaata ctatttacat atatctagtt caaagacaat aattattatg atccgttatt
       61 atctaagcac tatacagata ataggaattt ttcgtttcta aatattccaa tttgggaatc
      121 caataaaaat tttcgttcaa atacgtgtct attcgaactc ttttctagtt gttaattatg
      181 aagggggaag aaataaaaaa tacaatctat acacgaataa gaatattatc caaaaggagg
      241 ttttagaaaa gatttattat acgtaaaaag ctcaagacat tttcttagcc ataaggacat
      301 gtttgatatt taagaagata tatgaatttt cttttctaag tataaagata actatccatt
      361 atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct
      421 tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc
      481 ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta
      541 aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat
      601 aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt
      661 actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt
      721 gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt
      781 atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct ggggattaca
      841 agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt
      901 taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc cttcccaaag
      961 aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa agaagatttc
     1021 attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag atttagctct
     1081 ttgattcata agcaatcctg gagaactgga aaatatgatc aagattgtta tcaagaatgt
     1141 atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact caactaaggg
     1201 agtctgtgac ataagtaaaa agatagtttc a
//
LOCUS       DAJSVD010000265         1220 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSVD010000265 DAJSVD010000000
VERSION     DAJSVD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.276"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(91..1002)
                     /locus_tag="ON498_003058"
     CDS             complement(91..1002)
                     /locus_tag="ON498_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HCR3941235.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          357 a          250 c          176 g          437 t
ORIGIN      
        1 gtgggtttta aattaaaaag ctaacgttac cccaaaagtg ctagtacaat aatgctgttc
       61 attaatttcg ttggtttcat tccgccatcc ttacctcctt gaattagtat gtgttgatcc
      121 gggaaacact gtcacaatta caccgtctat attgtttcct gatatctcat aacgaagatt
      181 agtcggcaca tgaatggtgg tatttttagt aacaggataa taaaagatct catatttttc
      241 cccatttaca ctaacagaaa tggttttttc ttcttttagg taatccatat attcctcgag
      301 aacgaaattc ttttctttca taatcgcact atgtggtaaa ccaacatagc gaatatgcca
      361 tggttcatat tgaattcctg ttaactctgt tttgtcctct ggataacgta aaatgaaccc
      421 gtatttccaa gcattttctt ctatccactt tccttcaggg gctcgttcca ttttcgtcaa
      481 gcttgatcct acatctagtg ataaacctga attatgctca ctataacctg ctggtaaggc
      541 atactcagcc cccatttctt ggtaaagcac actttgctca tcaaagtctc gatagccact
      601 attaataata aaatgactaa cgccaccctt tacagcatca ttgaccatct ctgaaaattt
      661 ttgtgctatt tcttttgaca tataaatatt actatcaagc aacccgtatc catttattaa
      721 ttcgtcatgt ttagataaat tcacgatatc tgacttcaca ctttcttggc gaacaggata
      781 tttactattg attaatagca gatttccttg ataaacctgt tcttctgtaa ttgttttctc
      841 ttgggtattt tcagaagtcc cactattttc taaatgttct ttgggatttt gatcataatt
      901 ttgaaattcg actttttcct gagaaaacag tgcttcatta acgtagtcat aacctaagta
      961 tattaagaat aacaataaca ataaaaaaaa caacttcttc attttcagtc tcctccttaa
     1021 ctatatagtt ggaagtatga aatatttcta gaatcttccc cttaaacggc acaatctcgt
     1081 ttgagattgt gccgttttgc cccgtaaatt acagtcaatt atttcgccct actggattta
     1141 ctattatcac caatgtagat gaattttcat aagtgatacc catgctgaat ttaacgaggc
     1201 tgaactgcct gttgacaatg
//
LOCUS       DAJSVD010000266         1205 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJSVD010000266 DAJSVD010000000
VERSION     DAJSVD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.277"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..581)
                     /locus_tag="ON498_003059"
     CDS             complement(<1..581)
                     /locus_tag="ON498_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941236.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLG"
     gene            complement(591..>1205)
                     /locus_tag="ON498_003060"
     CDS             complement(591..>1205)
                     /locus_tag="ON498_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3941237.1"
                     /translation="RLGGLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLN
                     YAKKLEFSDDPNVYYVIQTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKP
                     QSFNLLNNSLEESFPKIIITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEE
                     QSDGDILDRSNKMKTMQYPTLQVDINHFNCTGLSKIQIYRNGLR"
BASE COUNT          363 a          247 c          181 g          414 t
ORIGIN      
        1 cctagacttt tttcatgacg taatgtgtta ttatctttta gaaactcacc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gcgaagggat agctggtttc
      181 gctaaaactt ttgcgttttg ccatgcctga gtagcagtga tcttttctgt tccgctgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ttttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgttt
      421 attgaataag taccctgaaa tatctcggct ccgtttagtt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcatttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaatgatt aatatccact
      661 tgcaacgttg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacatcagt tcctgctact
      781 aagccttcga aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
      841 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
      901 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
      961 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     1021 tggataacgt aatagacgtt gggatcgtca gagaattcta atttctttgc atagtttaac
     1081 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     1141 tagttatagt gcaatgtaaa cgacatatct tcaaacgagt aatcttgtac taagccacct
     1201 aacct
//
LOCUS       DAJSVD010000267         1205 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSVD010000267 DAJSVD010000000
VERSION     DAJSVD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.278"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..581)
                     /locus_tag="ON498_003061"
     CDS             complement(<1..581)
                     /locus_tag="ON498_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941238.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLG"
     gene            complement(591..>1205)
                     /locus_tag="ON498_003062"
     CDS             complement(591..>1205)
                     /locus_tag="ON498_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3941239.1"
                     /translation="RLGGLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLN
                     YAKKLEFSDDPNVYYVIQTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKP
                     QSFNLLNNSLEESFPKIIITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEE
                     QSDGDILDRSNKMKTMQYPTLQVDINYFNCTGLSKIQIYRNGLR"
BASE COUNT          366 a          249 c          175 g          415 t
ORIGIN      
        1 cctagccttt tttcatgacg taatgtgtta ttatctttta gaaattcccc atgaaatcga
       61 tctagaatag atccctcttt tccaccaaag aattctaaaa aattcgcctt ttctatcttc
      121 acattagcaa gcgtatctac taatgacgag aaagaaaact gtgaaggaat aactggtttc
      181 gctaaagttt ttgcgttttg ccatgcctga ctagcagtga tcttttctgt tccactgtca
      241 tatttattca acaccgattt tcttatatca ttgaaaatag gttcagcttt tacttctatc
      301 gtattgccta ttacagaagt ctttgcataa taaatccgta gacgctgttt tgctcgattt
      361 tcatctacat aacactgaat aatacgtcct tctacaatca aatctgcatt agttccgctt
      421 attggataag taccctgaaa tatctcggct ccgtttaatt tattgctaac agtagctgtt
      481 aaccagtctg acaaagcgcc taaaccttgc gtatcatata aatgttcagc taaattattc
      541 gcgtcgtttt tatcgtaaat agttattaaa ttatcgatca tctatttcac ctaccttaac
      601 ccattacgat aaatttgtat tttgctcaaa ccagtacaat taaaataatt aatatccact
      661 tgcaatgtcg gatattgcat ggtcttcatt ttgttggacc gatctaaaat atctccgtcc
      721 gattgctctt cgtagcaaag catcaaatca ctatcaatga ctacgtcagt tcctactact
      781 aagccttcaa aactaaacac ataatcattt aagatgaact ggcatgaagt agctgaagga
      841 gtgatgataa tctttggaaa actttcttct aaactattat tcagcaagtt aaatgactgt
      901 ggtttatcta cggttatagg tacatcttct tgaactcttg cgaatggttt cgcagtaata
      961 tttacatcga actctcccca ttcaacaata tcgttttctg catccccaat atcgatagtc
     1021 tggataacat aatagacgtt gggatcatca gagaattcta atttctttgc ataatttaac
     1081 caatgacgca tgatataaaa cgattgcttg aacgcttgat ggtcttccac atcctctaaa
     1141 tagttatagt gcaatgtaaa cgacatgtct tcaaacgagt aatcttgtac taagccacct
     1201 aacct
//
LOCUS       DAJSVD010000268         1201 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSVD010000268 DAJSVD010000000
VERSION     DAJSVD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.279"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            69..689
                     /locus_tag="ON498_003063"
     CDS             69..689
                     /locus_tag="ON498_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941240.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            693..1142
                     /locus_tag="ON498_003064"
     CDS             693..1142
                     /locus_tag="ON498_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941241.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          435 a          194 c          270 g          302 t
ORIGIN      
        1 ttgatcatcg caaccgttga tgatccaaag caaaatgtgt tcactcttga gaaagtcggg
       61 gtgatctaat ggctaaaact aaacgaatga tggaaacgga tgaaaaaaca ggtgtacaac
      121 gccaattctt tccaatcaca catgcttccg cagttcttgg gttagaagaa ataatagcag
      181 gagaagcaac agttttatct gttaatggaa aaattggagc cgtcgtcatt actaaagaag
      241 atttgggatt agagaatgtt ctcacagaat taccctatgc aagtgaagaa gatgacggca
      301 ttatcacggc agaaatgtat caaaaaattt taaacagtgg agaaggtgac tacgtgttgc
      361 cagttgccac tatcgaccgt ttgggtggca taaaagttgg tgaattattg acgattgatg
      421 agacagggaa agtctctgca gtcagacaat ctgatgtcaa tttttcgctg gagttaaaag
      481 aaaaactcga ttcactgaaa aattatactg ctggagaaaa catcactatt gatgaagatg
      541 gaacaattag ttctacaggt ggtagtggga ctggtggtgt caatcagtca tatgtagatc
      601 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
      661 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
      721 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaacgg
      781 tgaagggaga taaaggcgat cctggccaag cagcaacaat taccatagga accgtttcta
      841 gtggttcaac cgcttctgtt acgaatgtag gaacatcatc tgcagctaga tttaattttg
      901 ttttaccaaa gggagataaa ggtgatcctg gaataaatgc cacaactaca gctgtagcta
      961 caactacagc aaatgggttg atgtcgtcca cagataaaac taagttggat ggaattgcag
     1021 ctggagcaca gaaaaatcca ggtaatgcta caactacgac agcaggttta atgtcagcaa
     1081 ccgataaagt gaaacttgat ggattagcca atattacatt tgagaaagtg gggacggttt
     1141 aatggctgat attgttcaat taaaagaaga cggagttgct aaatacttaa aaacgcatgt
     1201 a
//
LOCUS       DAJSVD010000269         1198 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJSVD010000269 DAJSVD010000000
VERSION     DAJSVD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.280"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            69..686
                     /locus_tag="ON498_003065"
     CDS             69..686
                     /locus_tag="ON498_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941242.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            690..1139
                     /locus_tag="ON498_003066"
     CDS             690..1139
                     /locus_tag="ON498_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941243.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          437 a          174 c          264 g          323 t
ORIGIN      
        1 ttgattattg caactgttga tgatccaaaa cagaatgttt tcactcttga gaaagttggg
       61 gtgatttgat ggttaaagta aagagaatga tggagactga tgaatctggt gttaaacgtc
      121 aatttatgcc aattacacat gtttctgcag ttttaggctt agaaaaaatg atatcaggac
      181 aatcaaaagt tctttcggtt aatggtaaat atggtgcagt cattttaacg aaagctgatt
      241 taggattaga gaatgcaatt actgaactac cttatgcaag cgagacaagc gatggtattc
      301 ttactgctga aatgtttcaa aagatcgtaa atggagaagg tggaacatat attttgccga
      361 ttgctactcc tgaacaatta ggaggtataa aagttgggga actcttagaa gttactgaag
      421 aaggtgtttt atctgcaact aagcaaacag acttcaattt ctctgaagaa ttaaagggaa
      481 aattagaatc tctaaaaata ttaaaagcag gtgcaaatat atcaattgca gaagatggaa
      541 caattagttc tacaggtggt agtgggactg gtggtgttaa tcagtcatat gtagatcaaa
      601 aattccaaga agctgtaaat caagcagaaa attacacaaa cgaaagaatt ccaaatttta
      661 cttttgaaaa aatcggggag gtatagaaaa tgacagatat cgtaaaaata aaacaaagcg
      721 gtgtacaggt ttatccacaa actcattgga atgctataga aggcaaacca acaacggtga
      781 agggagataa aggcgatcct ggccaagcag caacaattac cataggaacc gtttctagtg
      841 gttcaaccgc ttctgttacg aatgtaggaa catcatctgc agctagattt aattttgttt
      901 taccaaaggg agataaaggt gatcctggaa taaatgccac aactacagct gtagctacaa
      961 ctacagcaaa tgggttgatg tcgtccacag ataaaactaa gttggatgga attgcagctg
     1021 gagcacagaa aaatccaggt aatgctacaa ctacgacagc aggtttaatg tcagcaaccg
     1081 ataaagtgaa acttgatgga ttagcgaata ttacatttga gaaagtgggg acggtttaat
     1141 ggctgatatt gttcaattaa aagaagacgg agttgctaaa tacttaaaaa cgcatgta
//
LOCUS       DAJSVD010000270         1196 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSVD010000270 DAJSVD010000000
VERSION     DAJSVD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.281"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(4..303)
                     /locus_tag="ON498_003067"
     CDS             complement(4..303)
                     /locus_tag="ON498_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941244.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(309..539)
                     /locus_tag="ON498_003068"
     CDS             complement(309..539)
                     /locus_tag="ON498_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941245.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(789..989)
                     /locus_tag="ON498_003069"
     CDS             complement(789..989)
                     /locus_tag="ON498_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3941246.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          422 a          197 c          174 g          403 t
ORIGIN      
        1 actttaatct ttgtatcttt ctggatcaac gaaagtatac tttatatagt cataacgccg
       61 atgatcgctt cgagcgtcta gcacatcagt cacgatatca aacaaaaaat atacgtcttt
      121 cttcattcta gttttcgcag cagggatttt aaagtagttc ttattagaat agtagagatt
      181 aattaataag ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa
      241 atctttgaca aatctattcg aaggatcaaa tttaaaccat aattgcgtct cattaaaaag
      301 cataaccatt actccgatct atttttaaac ttagtctcta cctctaatat atatcgagtt
      361 tttattttgc cttcagagaa tactgtttct ttttttgcag ttacaggttg tttattttcg
      421 gaaaaagcta atatagctaa aattgaaaca tccatctgga atttgtcttt ttcgttgctt
      481 tgctcataaa aatctgcata ttcatcacta atattttttc taataaattc ttccatcata
      541 aagatcacct cgaaaagagt atacgaacaa acgttctttt tgtaaaggtg gaatttatta
      601 tactaaataa ataggtgaac aagtacttgt gccaaattat gtgccaaaaa aaatcgaatt
      661 taataaaaca caaacaaaaa gaaatcctat tatgataaca tttcttataa taacaaacac
      721 aacagaagac gtgtaatagt tagtcaggaa cgtacaaata acccctgtat cctttgcggt
      781 acagggggtt attttgtata agtagacatt gtagtttcta aactgctatg tcctaaacgt
      841 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt
      901 agtccatcaa taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt
      961 actaggtcga cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga
     1021 ttggcttata ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac
     1081 ttgaagcttt acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt
     1141 aatagctgat gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaa
//
LOCUS       DAJSVD010000271         1137 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJSVD010000271 DAJSVD010000000
VERSION     DAJSVD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.282"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..355
                     /locus_tag="ON498_003070"
     CDS             <1..355
                     /locus_tag="ON498_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941247.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ON498_003071"
                     /pseudo
     CDS             615..910
                     /locus_tag="ON498_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ON498_003072"
     CDS             985..>1137
                     /locus_tag="ON498_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3941248.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAJSVD010000272         1106 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSVD010000272 DAJSVD010000000
VERSION     DAJSVD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.283"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            70..684
                     /locus_tag="ON498_003073"
     CDS             70..684
                     /locus_tag="ON498_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941249.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(849..>1106)
                     /locus_tag="ON498_003074"
     CDS             complement(849..>1106)
                     /locus_tag="ON498_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941250.1"
                     /translation="FYKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQE
                     LAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          369 a          196 c          189 g          352 t
ORIGIN      
        1 agtgacatat ggcttcctcg tgtatgatta gggtgtattt taaatagttg cagaaagtag
       61 gtaaaagttt tgagagttgc atacatacgt gtctcatcga tcgatcaaaa tgaacaacgc
      121 cagattgaag aaatgaaaaa attcggtgca gagagaattt tcatcgagaa acaatctggc
      181 gcaacgatta ctcatcgacc tgttttccaa gaagcattag attacgtccg agatcaagat
      241 atttttattg tggaagctat tgatcgactt ggaagaaatt atgacgagat catcgcttca
      301 gtcaattact tgaagaagaa aaatgtgaag ctgatcatta ctagtttacc gatcatggct
      361 gaagcaattg gcaatcctct cctggataaa ttcattaagg atctgatcat ccaaattttg
      421 gccatgatcg ctgaacaaga acggactgaa tcgaaacgtc gccaggcaca aggaattaag
      481 atcgcaaaag caaacggcgt ctacaaaggg cggcccaaat tatacagtgc agacgcaaaa
      541 gatccccaac gtcgccttgt ttacaaaagc atcgttgaag atcttaaaaa tggtgttgcg
      601 attgctaaga ttgctaaaga ctacaatgtt acgagacaaa ccgtgtatag aataaaaaaa
      661 gacagcatgg tcaatgacaa atagttgatc atgctgcttt tattatcttc tcatctttaa
      721 tttcctacat ataattacta tctataaaat taagtcttac tcagaacaga taaagcataa
      781 aattccaaca aaccattttt tacttaacta aataaagagt ctgttaatat aatcttcact
      841 tatttacgtt aatcccctac tgtatttgtt ggttccttat ctatattttc tacaaaatcc
      901 ttattatctt gtgatttagc ttcgtataat tctttgtatg tcgacacatt ggttatacta
      961 attcctatag ctagttcttg agactctact tttctctcta gctcctttga caaagtgatt
     1021 tgagatttat ttggttccgt tggagtcaat tctatagatt cttcataaaa atctctatac
     1081 ataaagctat cattaatttt ataaaa
//
LOCUS       DAJSVD010000273         1065 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSVD010000273 DAJSVD010000000
VERSION     DAJSVD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.284"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(39..995)
                     /locus_tag="ON498_003075"
     CDS             complement(39..995)
                     /locus_tag="ON498_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941251.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          347 a          178 c          159 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttagt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaac
//
LOCUS       DAJSVD010000274         1058 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSVD010000274 DAJSVD010000000
VERSION     DAJSVD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.285"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            232..315
                     /locus_tag="ON498_003076"
     tRNA            232..315
                     /locus_tag="ON498_003076"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:266..268,aa:Leu,seq:cag)
     gene            complement(639..>1058)
                     /locus_tag="ON498_003077"
     CDS             complement(639..>1058)
                     /locus_tag="ON498_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3941252.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          341 a          174 c          230 g          313 t
ORIGIN      
        1 aaaagaaatg aagaaaaaaa taaaatagtt actaatccca gagtcgttcg tttaacattg
       61 aaatcgatca tatggataaa acagattttt tcaccaaccc tcaatagatg atggagttgc
      121 tcggatgaat tatttgtgta gatggcataa gggagggcgt acagaaatga aaaagaacaa
      181 aaatatgttc ttgacaaaaa acacatgtaa tcctaaacta aaagatgtga tgcggctatg
      241 gcggaattgg cagacgcgct agcttcaggc gctagtgagg gtgacctcgt ggaagttcga
      301 gtcttcttag ccgcataatt tgttttttta taaagagaag gtacacctgg agcgatgaga
      361 aagaaaataa catattatct aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt
      421 caaataaatc aaaaaaaatt tgtgtgaagg tgggacagtt taaaacctaa tagattgatt
      481 caaatagtag atttatagat tctgatacaa gaaatactgg caaacgatag ttaaggaata
      541 aagactaaac ttaaactcaa aaagaggtca tgcgaaagaa taaatcgcat gacctcttac
      601 tatattttga atgtgtttaa acgttcaaat aatgaatctt agtaccaacc gtttgcttcc
      661 cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact
      721 ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgt
      781 ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat
      841 tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc aggagcaggt
      901 tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt
      961 tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca
     1021 gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAJSVD010000275         1053 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJSVD010000275 DAJSVD010000000
VERSION     DAJSVD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.286"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>1053)
                     /locus_tag="ON498_003078"
     CDS             complement(<1..>1053)
                     /locus_tag="ON498_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941253.1"
                     /translation="GVDVYHPQLKVNMSARIVEYTFNVLTNSYDKLVVGNVKTNFLEN
                     TENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNGGYVLLDPKEAPSRILI
                     MDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFNASFITAGEIVGITIRG
                     TTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSADAGIVSYTMKTGGGFMI
                     RNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGSSLSINGSAGNSWEFKV
                     AGRTMKFTSDGMLTLPGCFFGSWEDGKLARFEQSTVQVYKDFTVRGTKNSTVPTEHYG
                     QRLLNAYETPEYYFADY"
BASE COUNT          326 a          231 c          147 g          349 t
ORIGIN      
        1 ataatcagcg aaataatatt ctggagtttc ataagcgttc aatagtcgtt gtccataatg
       61 ttctgttggt acagttgagt ttttagtacc tctaacagta aaatctttat atacttgtac
      121 cgttgattgt tcaaacctag caagtttccc atcttcccat gaaccaaaaa aacaacctgg
      181 taacgttagc ataccatcac tagtaaattt catagtccta ccagctacct taaattccca
      241 tgagttaccc gcactaccat taatgcttaa agaactaccg tcgccagaag ttacataact
      301 agcattgcta tacctgaaat tgggcgcacc aaaagataga aacggtctgt tattaccatt
      361 atcccacgta ctaaaaacca agttaccctg tggatttcta atcatgaaac caccaccagt
      421 tttcatggtg tatgatacaa taccggcatc agcacttaca taatcacgtg cttctagctc
      481 cataatatct ttgttaactt tttttgagta ccaagtcatt ttgccattag caatacttgt
      541 tctataatca gcaccatcac taattaatgt agtacctcta atagtaattc ctactatttc
      601 accagccgta ataaacgagg cattgaatcc gccatctaac gtccatgccg tttcatatgt
      661 tccattaatg ccagttttag aaaaaccaat accagcattg ttgatttgta aaacattcct
      721 tgcggtattc ttatctggtg tgtccataat caaaatacga ctaggcgctt ctttaggatc
      781 taataaaaca taaccaccat tttgaccagt aatcatatca gtttgatgat ctacaatatc
      841 attgagtaaa tcactgattt cgccaccgtt tttcaattga tcaatggcat cattaatcaa
      901 attgctgaca ttattctctg tgttttctaa gaagtttgtt ttgacgtttc ctacaactaa
      961 tttatcgtat gaattggtta gaacattaaa cgtatattcc acaattctcg ctgacatatt
     1021 cacttttaac tgtggatgat acacatctac tcc
//
LOCUS       DAJSVD010000276         1038 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJSVD010000276 DAJSVD010000000
VERSION     DAJSVD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.287"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>1038
                     /locus_tag="ON498_003079"
     CDS             <1..>1038
                     /locus_tag="ON498_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3941254.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKH"
BASE COUNT          361 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacacc
//
LOCUS       DAJSVD010000277         1000 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJSVD010000277 DAJSVD010000000
VERSION     DAJSVD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.288"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..999
                     /locus_tag="ON498_003080"
     CDS             <1..999
                     /locus_tag="ON498_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR3941255.1"
                     /translation="FIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTS
                     IILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANS
                     KFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNES
                     DKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFL
                     KKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSK
                     VNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKGELI"
BASE COUNT          319 a          120 c          172 g          389 t
ORIGIN      
        1 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
       61 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
      121 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
      181 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
      241 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
      301 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
      361 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
      421 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
      481 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
      541 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
      601 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
      661 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
      721 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
      781 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
      841 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
      901 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
      961 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa
//
LOCUS       DAJSVD010000278          984 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJSVD010000278 DAJSVD010000000
VERSION     DAJSVD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.289"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            132..344
                     /locus_tag="ON498_003081"
     CDS             132..344
                     /locus_tag="ON498_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941256.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>984
                     /locus_tag="ON498_003082"
     CDS             506..>984
                     /locus_tag="ON498_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3941257.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFG"
BASE COUNT          370 a          130 c          155 g          329 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaa
//
LOCUS       DAJSVD010000279          966 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJSVD010000279 DAJSVD010000000
VERSION     DAJSVD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.290"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(264..953)
                     /locus_tag="ON498_003083"
     CDS             complement(264..953)
                     /locus_tag="ON498_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3941258.1"
                     /translation="MDNFILFHDKVFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDL
                     NHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNE
                     IVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDG
                     QYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQ
                     KIKEKLKESTV"
BASE COUNT          315 a          172 c          115 g          364 t
ORIGIN      
        1 ccaaatcttt cgcatcttcc accatacatt taattttaat tcccctttat ggtacttaat
       61 aaaaacaaga aaagaactat tcatttggcc cttttttggt tccagaccat agaaatattc
      121 ctccagtaac taatattgct gttatgcacg taactaataa cagctataaa aattggtaca
      181 actttaatag gattcagaaa aatttgcact agtgaaggaa tctattttat tagaaaaatg
      241 caatctccct aaagaaaaaa ccactaaaca gtgctttctt tcaacttctc cttaattttt
      301 tgtgctttcc ctaaagtaaa gtttctggcc aactctgaat caaaatatag tgaacgtttt
      361 ttaaaatcta tttctttaac gttaaagata tttactaaac acgctcgact aattcttgtt
      421 aacataggat atttcttttc aagctcacta attcttccat aaaattcgta ctgcccatct
      481 ttagtacaca atgataatct atgtggtaat tttgaagctt ctacaaaata aatgtcattt
      541 atatcaaaag taaatgtttg agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt
      601 tttgttgcat ctatcctttc ttgcgctaat aacaataact caacaatctc gtttcgatac
      661 tcatctagtg tttgatcttt tgttacaaat cctaacgttt caactcttct ttttattgtt
      721 acgggtaaca tctcatcatg agtagttgta aaaatgattt ttgcttgaac atcatatttt
      781 ctgattactt ctgctaattc tataccgtta acttcatgat ttaaatcaat atctaagaaa
      841 tatattcctt gctttggtcg gaattgtttg aggtattttt taacttctaa cggactttgt
      901 gttttcaata ctatcttaaa tactttatcg tgaaaaagaa tgaaattatc aataattcgt
      961 tctaac
//
LOCUS       DAJSVD010000280          949 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJSVD010000280 DAJSVD010000000
VERSION     DAJSVD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.291"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..174)
                     /locus_tag="ON498_003084"
     CDS             complement(<1..174)
                     /locus_tag="ON498_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3941259.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSD"
BASE COUNT          292 a          193 c          161 g          303 t
ORIGIN      
        1 atcacttaac tcttttaatt cctcgaaaga aaatctaact tttttcaaac cacgatcttt
       61 catacgagcg cataatgcaa aaaataaatc catctgctta gcattgaaat ttttcagcgg
      121 tactaaattc aattcatttt gatatttgac tgttaattcc gttttttcat ccattccttt
      181 cttcccccct tacaaataaa taataacctt taacaaccaa ctcgtaaata ggttggttgt
      241 tattatacaa aaggatatta gtactcagat aaaaagttgt tagtactcag ataaaaagtt
      301 gttagtactc agataagaag ttgttagtac tcagataaaa agttgttagt actcagataa
      361 gaagttgtta gtactcagat aaaaagttgt tagtaccctt ctgtatccgt tgggagagta
      421 aggctcaact gccccctaaa aagggttcta aaaaatcttt aaaaaataaa taaaataaac
      481 gcgcgcgcgc gagcaaaatt tcccgccaaa aaatcaccca ctttttaaaa taaacaggtt
      541 cgcgcatgat attaaactgc caatctagct ccctatggtc gctgtaagaa ggagtgatcg
      601 ctacgctctc acattttact tgcagcagtc gcctttaggc tccttcgcca aaaataattc
      661 attagggact cctttgtggc gtcctgagag ctcgtctaag ccgttctaag acaagattcg
      721 ataaaaacta tgtccttcgt tctaaagcgc cttacaggcg gtcagggcaa gccctaacaa
      781 ccccaaggca ataaaaaact taggcttatt tttgcttttt tattgtataa ttgattgtcg
      841 cctatccgac agtcttgcga ctggaaggag gtgtgtttcg tgttcactaa ctttatttgc
      901 tcccttcttg ttgggctttt aagcgctttg ttcagttact ggttgaata
//
LOCUS       DAJSVD010000281          920 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJSVD010000281 DAJSVD010000000
VERSION     DAJSVD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.292"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>920
                     /gene="bsh"
                     /locus_tag="ON498_003085"
     CDS             <1..>920
                     /gene="bsh"
                     /locus_tag="ON498_003085"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3941260.1"
                     /translation="DHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGI
                     AAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTV
                     GEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTN
                     NPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKAT
                     FTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYY
                     RTYEDSQITAIDMNKEDLDSHKLISYPIIE"
BASE COUNT          319 a          121 c          167 g          313 t
ORIGIN      
        1 tgatcattat tttggaagga attttgatta tgaaatatct tacaatgaag tagtcactgt
       61 tactccaaga aattataagt tgaattttcg aaaggtaaat gatttggata ctcattatgc
      121 aatgattggt attgccgctg gtatagctga ctaccctctt tattacgatg cgacaaatga
      181 aaaaggattg agtatggctg ggctaaattt ttctgggtat gctgattata aagaaataca
      241 agaagggaaa gacaatgtat ctccttttga atttattcct tggattttag gacaatgctc
      301 aacagtagga gaagctaaaa aattgttaaa aaatatcaat ttagcaaata taaattatag
      361 tgatgaactt cctttatccc ctttacattg gctattagct gataaagaaa aatcaattgt
      421 cattgaaagt atgaaagatg gacttcatat atatgataac cctgtgggcg ttcttaccaa
      481 taatccttca tttgactatc aattatttaa tttaaacaat tatcgtgtct tatcgagtga
      541 aactcctaaa aataattttt caaatcaaat aagtttgaat gcctatagcc gcggtatggg
      601 agggataggc ttgcctggag atttatcctc agtatctcgt tttgttaaag cgacttttac
      661 gaagctgaat tctgtatctg gagattcaga gtcagaaagt attagtcaat ttttccatat
      721 cttaggttca gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa aatatgaata
      781 tacaatttat tcttcttgtt gcaatgttga caaaggaatc tattattatc gaacatatga
      841 agacagtcaa attactgcaa ttgatatgaa taaagaagac ttagatagtc ataagttaat
      901 tagttatcca attatagaaa
//
LOCUS       DAJSVD010000282          918 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJSVD010000282 DAJSVD010000000
VERSION     DAJSVD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.293"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..865)
                     /locus_tag="ON498_003086"
     CDS             complement(<1..865)
                     /locus_tag="ON498_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941261.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKK"
BASE COUNT          312 a          107 c          157 g          342 t
ORIGIN      
        1 attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata tctgcaattt
       61 ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt tttagttctt
      121 taaaatagct aataacactt tttccattca cttttctaaa atgttttttt aagctggctg
      181 gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa agatgttctt
      241 ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc ttttttttag
      301 gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga taatgccttg
      361 gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa agcaaatgaa
      421 tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag gaaatagctg
      481 gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa gtaatgatag
      541 atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat aattgctcat
      601 caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa aagatttgga
      661 cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata gatttatgtg
      721 caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag ggagcagcag
      781 ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga ttggaggatt
      841 ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa gctttctatt
      901 tataattaat gactatta
//
LOCUS       DAJSVD010000283          886 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJSVD010000283 DAJSVD010000000
VERSION     DAJSVD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.294"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            362..>886
                     /locus_tag="ON498_003087"
     CDS             362..>886
                     /locus_tag="ON498_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3941262.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          322 a          126 c          158 g          280 t
ORIGIN      
        1 aatgagacaa aacattgcaa gattccttac taaattagtt tctgaatgga aaagtattat
       61 ttctcaaaat atggataaat taagatatta aggatgttag ttttaaaata aactaatgtc
      121 cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt gatgtagcca
      181 gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt ttgggtatac
      241 tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt tttctttatt
      301 gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg aatgatggca
      361 aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc ggcaacataa
      421 gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa aaattttcga
      481 ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc ttatttcttt
      541 catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa gaaatagtaa
      601 agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg aaatattaga
      661 cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa caaatttagt
      721 catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag agcgtcaaaa
      781 acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga ttcaatacca
      841 tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgtt
//
LOCUS       DAJSVD010000284          881 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJSVD010000284 DAJSVD010000000
VERSION     DAJSVD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.295"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..752
                     /locus_tag="ON498_003088"
     CDS             <1..752
                     /locus_tag="ON498_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3941263.1"
                     /translation="KLTTERVRIDQSQYKVSLLKSLKGLVKNRALLSIIGVAIFLLLA
                     QLLIMSMNNYVFPDYYNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGM
                     FFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQRE
                     DGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSIC
                     FLIVGLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT          286 a          104 c          160 g          331 t
ORIGIN      
        1 ataaactaac tactgaacga gtaagaattg accaatctca atacaaagtt tcattgttaa
       61 aatctttaaa agggttagtt aaaaataggg cattactttc tataattggt gtggctattt
      121 tcttactcct tgctcaatta ttaattatga gtatgaataa ttatgtattt cctgattact
      181 ataatgatgc acagggaata gttttaatga attttatcaa tcctattttg gttttaatag
      241 ttgtttcgcc tttaactttg tttttaagta aaaaatatgg aaaaaaagaa ctggcatcag
      301 taggaatgtt cttttcagca atagtatatt tgatcttatt tttgataaaa gctagtaata
      361 tgtatgtatt cttaattctt agttcggttg gctatatggg tttgggtgtg tttaatactg
      421 ttatttgggc aaatattact gatgttattg atgatcaaga agtaaagtct gatcaaagag
      481 aagatgggac cgtatatgcg gtatattctt ttgctagaaa aataggtcaa gctctagctg
      541 gaggcgtcgg aggttggact ctttctatta taggctatga ctctttagca aatgtacaaa
      601 cgggagatgt tttagaaaag ttatataacg cttcaacatt aattccatca atttgttttc
      661 tgattgtagg attaatttta tttttccttt atccattatc taaagataaa gtcttagaaa
      721 attgcgagct tttaaaacaa aaacataaat aggagagaaa tatgaataat tggatatttt
      781 gtattgattc agatggctgt gttatggata cgatgactta taaacacaat ctctttttcg
      841 gaccaattgc tgcagagatt tttgatgttc atcatagaga a
//
LOCUS       DAJSVD010000285          843 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJSVD010000285 DAJSVD010000000
VERSION     DAJSVD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.296"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            238..480
                     /locus_tag="ON498_003089"
     CDS             238..480
                     /locus_tag="ON498_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HCR3941264.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            477..>843
                     /locus_tag="ON498_003090"
     CDS             477..>843
                     /locus_tag="ON498_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3941265.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
BASE COUNT          315 a           94 c          163 g          271 t
ORIGIN      
        1 aaataagaaa ttgattgtcg ttttcttttg cagaaattta agtgcaggca attatggtag
       61 ttgtcgtaat tgctgtcagc tcagtgttta tcactgatgg tgtgaaagat ttacggttgg
      121 agatgttcaa taagattatt ggtgcttaat tatttttatt gtagtgctga tatgagaatg
      181 ttataattgg ggtataaaac atacaaataa atttgctgaa aaatgaggta tcaaaatatg
      241 gaaaatgaat tactggaaaa cttaggaaga cttcacacaa ctgaacttgg cgttattaga
      301 attaaaaaga acttatcttt gaatgtagaa aatgtaattg aatggtgcaa agaaaaaatt
      361 agcctttcta atgcggaaat cacaagaaaa ggaaaaaact ggtatataac gattgataat
      421 tgtattataa cggtaaatgc gtacagttat accgttatca ctgctcacaa ggtaaaatga
      481 taaatttttc tataaaggaa ttaaacgcat ttgcagttat tgggcaggaa gtagaactga
      541 ctaattatca aaaaaagaat attcagatta gtacacagtt ttggagaaag tttaacagta
      601 gtttgaaaaa agcgtatctt tcacaatcag gaaattgggt aaaatatgct tttatggaaa
      661 gaagaaatgg aaaactttat tatttctgtt ctattcccca aagaaccatt accccagagg
      721 gctttctgta taaagaaata ccgtcttata aatatttggt tgtggagcat attggtgcta
      781 tggataaaat atatgaaact tatggcaaga tttatgaaga aattattcct agtacatcgt
      841 ata
//
LOCUS       DAJSVD010000286          836 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJSVD010000286 DAJSVD010000000
VERSION     DAJSVD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.297"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            20..256
                     /locus_tag="ON498_003091"
     CDS             20..256
                     /locus_tag="ON498_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR3941266.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            275..490
                     /locus_tag="ON498_003092"
     CDS             275..490
                     /locus_tag="ON498_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR3941267.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          260 a          109 c          212 g          255 t
ORIGIN      
        1 aaatattatt taaagaagcg tgtttaaaca tgcaaaatgt aaaggaacta agtattgtgg
       61 aaatgcacaa actgttggtg gaggcaataa tgcttgggga aaattggggc aagttgtagg
      121 tggacttacc acaggggcag taggtggtgc aggtttagga acagcaattt gcggaccagc
      181 ttgcggtgtt gttggagggt tatatggagc tgtcgctgga ggtgctgcag ctggctggga
      241 tgctcgaaaa aaataaaaaa ggagaataag tagtatgcat attaaaaaca caaaaacaac
      301 atttatatta tcttcagaag agttgaaaaa tattcaaggt gggagtgccg tgggagtttt
      361 aggcactact ttttctgggg caacagcagg agtaaaactt tgttcagccg gaggacctta
      421 tgcaatagct gcttgtggtg taggtggagc tttattaggt gctggattca gcatgtggac
      481 cggtgcgtaa acaattaaaa aatatcaaac ctcaagattg gtttgtgtcg attacgaaca
      541 taggtatgtt ctttatcttt tcaaaagttt ggaatggaca gaacttttta ctaaagcttc
      601 ttgtgctagt ttttataatt atattaaatt tttatctttt attaaagtca gataaaaatt
      661 ctttggaaat gtaatggtag agatattttt acaaaaggac ttgcagcgct gtagttggtg
      721 ctgcaggagg acctatttcg gccgttacag gaggtattgg taatgtactt gtcggatgtg
      781 cattaaagcc tgttcctgca agttaaattt ataaaaacag gaaagtaatt atctca
//
LOCUS       DAJSVD010000287          834 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJSVD010000287 DAJSVD010000000
VERSION     DAJSVD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.298"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..469
                     /locus_tag="ON498_003093"
     CDS             <1..469
                     /locus_tag="ON498_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HCR3941268.1"
                     /translation="NQLNDENKVATYEFASNRLEEQKQENVLDFPKKEKIPTVHNSAS
                     AANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDG
                     EIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYEDRIVSPEQIRIIGKVVF"
     gene            556..>834
                     /locus_tag="ON498_003094"
     CDS             556..>834
                     /locus_tag="ON498_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HCR3941269.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTS"
BASE COUNT          322 a          115 c          165 g          232 t
ORIGIN      
        1 taatcagctt aacgacgaaa ataaagttgc tacctatgaa tttgcaagta atcgattaga
       61 agaacaaaaa caagaaaatg tcctagattt tcctaaaaaa gaaaaaatcc caactgttca
      121 caattcggct tcggctgcca atccaacaga acttacttac ggtgatacgg ttgtcgaaga
      181 ggaagaattt gagagagttc caagtgctgc tgacttcgca gtacctatta taggcgattc
      241 aatggaacct gttatcagga atggacaatt cgtattcgtt aaagaacaac cggacgtaga
      301 agatggggaa attgctattg tggaattagg tggtgacgga gttacatgta aagagatata
      361 taaagattac gaaaatcaaa caatcatttt acgttctata aacgacttat acgaagatag
      421 aattgtatcg ccagaacaga ttagaattat cggaaaagtt gtattttaaa aaaattccca
      481 tgccggagcc gtcaacttcg gtaagggttt atatgatttt gacaattatt ttataaaaga
      541 agcgaggcgt tttgaatggg agtactattg tttttagttg gaatagtttt aataattatg
      601 ggtagtagca aattatctga agctaaaaaa ttaaaagata aaacttctgc cttagagagt
      661 aaggtaaagg aatattctca agaattaaat gctagggaaa cgtttatatc tgaaaaagag
      721 aaaaacataa gtaaattgaa taaaaacttg tcaacgctag aaaacaaaat tgatattctt
      781 aatgctgaaa gccagcggtt agacgatgaa attaatcaaa aatatgacac atct
//
LOCUS       DAJSVD010000288          832 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJSVD010000288 DAJSVD010000000
VERSION     DAJSVD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.299"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            199..832
                     /locus_tag="ON498_003095"
                     /pseudo
     CDS             199..832
                     /locus_tag="ON498_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          276 a          157 c          160 g          239 t
ORIGIN      
        1 agcaagctag tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa
       61 tcattatgga tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat
      121 tcgcaaaata taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc
      181 cattgttatt ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag
      241 ccgtcaaaaa ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca
      301 attgtggata aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt
      361 ttgcctacac gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa
      421 gcggtggaat atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc
      481 ttaccaaatg cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg
      541 ttcaatgatt ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa
      601 gccgagaaag tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac
      661 tatgattaca aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca
      721 atgatgattg atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc
      781 catgagctga ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAJSVD010000289          819 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJSVD010000289 DAJSVD010000000
VERSION     DAJSVD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.300"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..739)
                     /locus_tag="ON498_003096"
     CDS             complement(<1..739)
                     /locus_tag="ON498_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR3941270.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKN"
BASE COUNT          237 a          178 c          141 g          263 t
ORIGIN      
        1 agttcttgcg aacagcctta actaaccctt tgtgtgcatc agaaatgacc agttttacac
       61 cgaacaaacc tcgttgaacg aacgattgaa aaacggtatc ccaattatct tccgattcgc
      121 cgtcttgaac atcaaaacca agaatcgtac gtcgtccatc agaatcaatg ccgataccaa
      181 tatatacacc ttttgagaca atacgatgat tttcccttac tttcatatac agggcatcac
      241 acatgaaaaa tggatattcc gagccttcta ggctccggtt tctccactca aaaacaaatg
      301 gatccaactg tttcatcaga tttgaaacga atgatttaga aacagtcgca ccatccgtta
      361 gcagttcaac tgttttagtt actttgcgag tggagacacc agaaatgacc atttcaatca
      421 tggcagcgat caaagctttt tcgcttcgct gatatctttc aaagatagta ggactaaact
      481 taccgtctct ggttcgtgga acatgaagtt ctaacgtacc aattcgagtt gtaaacgaac
      541 gatcgtagta accatttctt tgtgattgcc gttcttctga acgttcatag gcatttgcct
      601 gaatatattg atttcgttgt tcttccatca gctgattaaa catgatagtc ataagtgttt
      661 ttgcggtatc attcgcaccg ctttcgttta taattgcttg aatttcttcg ctttcaaaag
      721 taaaatgtac ttgagtcatt gatatcagtc ctattcgagt atgttttctg caaaaaacat
      781 tgtactccta actgagatca atggcttttt tgatgcttc
//
LOCUS       DAJSVD010000290          789 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJSVD010000290 DAJSVD010000000
VERSION     DAJSVD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.304"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            135..767
                     /locus_tag="ON498_003097"
     CDS             135..767
                     /locus_tag="ON498_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3941271.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          284 a          110 c          127 g          268 t
ORIGIN      
        1 gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc
       61 tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact ttatgaaaat
      121 ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat aaaggatcct
      181 tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca caaaaagagg
      241 tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc aagtatgcat
      301 tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc gaaaactgtg
      361 atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta
      421 aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc gagaattgta
      481 aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt gagcactctg
      541 tcttatcaag cgcagatttt gtagactgtt ccctcactaa aataatgttt tctactaatg
      601 atttagaaaa tgctagcttt tttgatactg atttaaaaaa tatcgattta agtagcaact
      661 cctttgaaat gattgaagta cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc
      721 aagcacttgg ttttgctaga aaatttttac aaataaaagt agtataatca aaatgaatat
      781 ataagataa
//
LOCUS       DAJSVD010000291          787 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJSVD010000291 DAJSVD010000000
VERSION     DAJSVD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.305"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..342
                     /locus_tag="ON498_003098"
     CDS             <1..342
                     /locus_tag="ON498_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941272.1"
                     /translation="NTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESN
                     PQMSTEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLD
                     ILYKNTIFTNL"
     gene            375..>787
                     /locus_tag="ON498_003099"
     CDS             375..>787
                     /locus_tag="ON498_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941273.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNP"
BASE COUNT          315 a          113 c          139 g          220 t
ORIGIN      
        1 aatacaccta attcaattag tttgagcaat gaaatcacta atgaacagaa atttacgtat
       61 tcgccatggc tatatagcga actgacgaca gcttcaatag tggaaaaaaa gaatccacga
      121 ttggaaagta atcctcaaat gagcaccgag gatgttagaa gcattataaa gcactattct
      181 tcgcaagaaa atctgcaaat aaaatattct ttaaatttaa gtgaattaac ttatttacga
      241 aaagctgatt tgaaaaaatg ggctttagat gctgtggaat gcactaaagg aagtaataca
      301 ttagatattt tatataaaaa caccattttc actaatctct aatcaattta ctgaatcgaa
      361 aggaaccggg tagaatggat aagaaagttt taataaagag gttctctgaa gctattgaaa
      421 aaggaaatgc agcaatattt gcaggtgcag gattatcgat gtcacaagga tatgtaagtt
      481 ggccggaact acttaatgat cctgcaacag aaatcgggtt ggattctaaa aaagaaacag
      541 acttagttac tctggctcaa tattataaaa atgaaaatgg tggatcaaga ggaattttga
      601 atcagatact tatggataat tttggtgaag aattagaaat atctgaaaat catcgaatac
      661 tagcaagttt acctattgaa acttattgga caactaatta tgatcatcta atagaaaaat
      721 caatccgaga agcttataaa aaccctcagg ttaagaaaaa ttatactcag ttagcaacaa
      781 ctaatcc
//
LOCUS       DAJSVD010000292          785 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJSVD010000292 DAJSVD010000000
VERSION     DAJSVD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.306"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..142
                     /locus_tag="ON498_003100"
     CDS             <1..142
                     /locus_tag="ON498_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941274.1"
                     /translation="IIDKLLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSM
                     GI"
     gene            583..>785
                     /locus_tag="ON498_003101"
     CDS             583..>785
                     /locus_tag="ON498_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3941275.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT          265 a          152 c          156 g          212 t
ORIGIN      
        1 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
       61 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
      121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
      181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
      241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
      301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
      361 tctcaccaaa aggttcgttc ctgttcgtta tctctttatt gatttgccag accggcaaca
      421 agttgccttt tatcaccaaa aagcgacaag gacaagggta aatggcacgc tgcgcgccct
      481 tgtccttgtc gcttttcgtt gatttattct ggccaacaaa tcaataaaga aagaactaac
      541 gaaaggaagt aaaacctttc ctaagaggag gcagaatcaa acatgcgaaa gaaatttgat
      601 tattggggtg ttcccttcag cgaattattc ccaaactatc atgcaccaca tactgttgaa
      661 tgcgattgtg gagaaagagc gaaatgtatc aaaagctatc gtttgtatca atgtccaaca
      721 tgcgggaaaa aatatacctt gtcttatggc gattacattt tgattgatga gaaaggaaag
      781 aaacg
//
LOCUS       DAJSVD010000293          771 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJSVD010000293 DAJSVD010000000
VERSION     DAJSVD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.307"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            1..771
                     /locus_tag="ON498_003102"
                     /pseudo
     CDS             1..771
                     /locus_tag="ON498_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          294 a          130 c          145 g          202 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt a
//
LOCUS       DAJSVD010000294          738 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJSVD010000294 DAJSVD010000000
VERSION     DAJSVD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.308"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..>738)
                     /gene="aph(2'')-I"
                     /locus_tag="ON498_003103"
     CDS             complement(<1..>738)
                     /gene="aph(2'')-I"
                     /locus_tag="ON498_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR3941276.1"
                     /translation="FSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSIL
                     GYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLE
                     EYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRL
                     TGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEE
                     YYPIETIVYGIKNIKQEFIENGRKEIY"
BASE COUNT          248 a          121 c           51 g          318 t
ORIGIN      
        1 tataaatttc ttttctacca ttttcgataa attcctgttt aatattttta attccataaa
       61 caatagtttc aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa
      121 tatctatatt tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt
      181 cactatcttc aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa
      241 aatcaattat tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat
      301 cattatggca taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa
      361 aactttctat ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca
      421 atatatactc ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat
      481 aatctaaacc gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt
      541 cttcttctga catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat
      601 aacctagtat agataattca tcactaatat acgaatattc aatattagga atttttacat
      661 tagtttctaa atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt
      721 tcttattagt actaaatt
//
LOCUS       DAJSVD010000295          697 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJSVD010000295 DAJSVD010000000
VERSION     DAJSVD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.310"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..667
                     /locus_tag="ON498_003104"
     CDS             <1..667
                     /locus_tag="ON498_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3941277.1"
                     /translation="GKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYR
                     WVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKK
                     RDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNL
                     IEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IPA"
BASE COUNT          239 a          122 c          139 g          197 t
ORIGIN      
        1 aggaaagcaa tttcagcagg atgtgattat tgtagccgtg ggctactatc ttcgttataa
       61 ccttagctat cgtgaagttc aagaaatctt atatgatcgt ggcattaacg tttctcatac
      121 gacgatttat cgttgggtgc aagaatatgg caaactactc tatcaaattt ggaaaaagaa
      181 aaataaaaaa tccttttatt catggaaaat ggatgaaacg tacatcaaaa ttaaaggaaa
      241 atggcattat ttgtatcgag ccatcgatgc agatggttta accttggata tttggttacg
      301 taaaaaacgg gacacacaag cagcctatgc ttttcttaag cggttagtga agcagtttga
      361 tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact
      421 aaaagaatac ggcttttatc aagggacaga acatcgtacc attaaatacc tgaataattt
      481 gattgaacaa gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
      541 tgcctctacc acgattaaag gcatggaagc cattcgagga ttatataaga aaacccgaaa
      601 agaaggcact ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc
      661 agcttaaatc atagataccg taagggattt tattctt
//
LOCUS       DAJSVD010000296          696 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJSVD010000296 DAJSVD010000000
VERSION     DAJSVD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.311"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          238 a          132 c           92 g          234 t
ORIGIN      
        1 gtctcacgct ctaaacacgt ataaacagcg gaagcagaag caactccttc ggaaataagc
       61 tgaaattcac aaaaatttga aaagcaattt ccgtgaattt cttcttattt cttggagtta
      121 aacgcttctg ttccagcctc tttttatctg tctttcacta atgatcagtt gtagataagc
      181 aagttgcttt ttcttacatc ataccgccca tcaagacaaa cgtttttagc ttgtacgtac
      241 tgggcaaaaa tcccattata tcaaggaaaa acaacattga tagtacttaa aattattagg
      301 ctagaacgga ctagtaagag ttttttgccc cttttttgcc ccctctatct acaataaaaa
      361 tattatccat tcttatatga ccctatataa ataataaaaa gaggagaaaa aatatctcct
      421 cttttttcat attactacac caacctaaaa aggaggcgaa gcgaggtttc tacctcataa
      481 gttgaattaa tactatcatt caacacagaa ttcgtcaaga atttttgtta atgaattaaa
      541 aaaattctta atgtgcatat tttgttcatg tagttctaaa gttttattta ctctttctaa
      601 atagttagaa aaatctttat ttctgtgtga acgcatccct tctgaacaca taattctatg
      661 tagttcaaac aaagcaaata tccttgcaac attcaa
//
LOCUS       DAJSVD010000297          689 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJSVD010000297 DAJSVD010000000
VERSION     DAJSVD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.312"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            82..>689
                     /locus_tag="ON498_003105"
     CDS             82..>689
                     /locus_tag="ON498_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR3941278.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DAJSVD010000298          661 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJSVD010000298 DAJSVD010000000
VERSION     DAJSVD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.313"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            73..>661
                     /locus_tag="ON498_003106"
     CDS             73..>661
                     /locus_tag="ON498_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941279.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLI"
BASE COUNT          237 a           94 c          126 g          204 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 a
//
LOCUS       DAJSVD010000299          657 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJSVD010000299 DAJSVD010000000
VERSION     DAJSVD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.314"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            34..>657
                     /locus_tag="ON498_003107"
     CDS             34..>657
                     /locus_tag="ON498_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3941280.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTT"
BASE COUNT          173 a          111 c          124 g          249 t
ORIGIN      
        1 tgctgaaaat tttcaaaaga aaagaggcta gaaatgaaaa aattaaattt atttacagaa
       61 ttgtgcggaa tcattttagg cgcaggaatc tacggattag ctgtgacagg aatcaatatt
      121 ccatctaagc tggcagatgg tggggttact ggtattgctt tgcttttgca taatcttttt
      181 ggattcgctc cttctgtcac ttcactgctt tttaatttac ctttattgct tatctcattg
      241 tttctttttg gaaaacacgc ttttctccgg acgatcgtcg gaacatttgc gctagttttc
      301 tttttacatt tatgggagag ccttcatata cattttgctg tagatagtct actgatcaat
      361 agtttgatga caggtattct ttctggcatt ggttgcggtc tcgtctttcg attcggcgga
      421 agcacaggcg gaacagatat catttatcaa gccattgaaa aatattttca tattaagatt
      481 ggcaaaagtc ttttcttgat tacttttggt attttagttg tctccttgct ttatttggat
      541 ctcacacatt ttctgtatac attacttagc tgttctattc tatcttatac attgaataaa
      601 gtgaagtatc tggaaattgg atctgttttg ccccaattgg aagcaaaaca gactaca
//
LOCUS       DAJSVD010000300          656 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJSVD010000300 DAJSVD010000000
VERSION     DAJSVD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.315"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ON498_003108"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ON498_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3941281.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="ON498_003109"
     CDS             complement(481..>656)
                     /locus_tag="ON498_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941282.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAJSVD010000301          646 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJSVD010000301 DAJSVD010000000
VERSION     DAJSVD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.316"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            221..619
                     /gene="spxA"
                     /locus_tag="ON498_003110"
     CDS             221..619
                     /gene="spxA"
                     /locus_tag="ON498_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3941283.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          230 a           96 c          122 g          198 t
ORIGIN      
        1 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
       61 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
      121 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
      181 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
      241 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
      301 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
      361 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
      421 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
      481 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
      541 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
      601 attgatggct ggattataga aggataatac gaaacttgga aaagac
//
LOCUS       DAJSVD010000302          616 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJSVD010000302 DAJSVD010000000
VERSION     DAJSVD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.317"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>616
                     /locus_tag="ON498_003111"
     CDS             <1..>616
                     /locus_tag="ON498_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR3941284.1"
                     /translation="DMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNE
                     LRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGF
                     STSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNA
                     MIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIK"
BASE COUNT          217 a          124 c          113 g          162 t
ORIGIN      
        1 agacatgaac gccgcctact tccagcttac caaacgtatt ctgccaaatg cgaaagtagt
       61 gattgatcgg tttcatatta tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg
      121 tgaaatgaat gaacttcgta aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa
      181 ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa
      241 aagttttcga gcaccaaaat acccatttct tacggaagcg atgatgattg atcgattgct
      301 tggcttttct acgtccctga aggaggcgta tccctatttt catgaattag ttgaagcctt
      361 tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga
      421 tgacggcttt cgggaaaagc tccaaaacct tctgacctat gaagaaggca tcaccaacgc
      481 gatgatctat ccttacacca atggaaaaat agaagcgaag aatacccata taaagacaat
      541 gaaacgagta tcctacggat ttaaatcatt tgagaacatg agaattagaa tctttttgat
      601 caatcaatta atcaaa
//
LOCUS       DAJSVD010000303          593 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJSVD010000303 DAJSVD010000000
VERSION     DAJSVD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.319"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..580
                     /locus_tag="ON498_003112"
     CDS             <1..580
                     /locus_tag="ON498_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3941285.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAJSVD010000304          584 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJSVD010000304 DAJSVD010000000
VERSION     DAJSVD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.320"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            181..>584
                     /locus_tag="ON498_003113"
     CDS             181..>584
                     /locus_tag="ON498_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941286.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDL"
BASE COUNT          179 a           89 c           81 g          235 t
ORIGIN      
        1 tttagctccc ctattaattt tgaaacgttt agatcatatg gcatgcaagt aaataatttt
       61 tacccatggt taactctata tcctttattt ttattgatca agtttactaa tttagcaata
      121 ggctataacc tttttcttta tattgttacg ctcattactc tatttatatg tcattatgta
      181 atgtatgaaa ttaccaaaaa acatgttact agctcctttt tcgctattat atatacaaca
      241 tcttctttta ggagtgtaga aatatttctt cgaggggcaa tgggggaatt actcgcaatg
      301 tcaatacttc ctcttatttt actcgggttt ataaagcttt acgactcaaa aaaagaatct
      361 tgggttatgt tggcgattag catgacttta ttaatttaca ctcatgttct ttctgtagct
      421 atgactatgg taatgattat aattgcttta attattcaat tttatcggaa aaagaaaata
      481 gaaatcttct taattattaa attaataaaa gctgctatag ttactttact tctttcacta
      541 gcgtttatag gtcctatgat tgagcagaca ttgtatcaag atct
//
LOCUS       DAJSVD010000305          578 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJSVD010000305 DAJSVD010000000
VERSION     DAJSVD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.321"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            126..413
                     /locus_tag="ON498_003114"
                     /pseudo
     CDS             126..413
                     /locus_tag="ON498_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..>578
                     /locus_tag="ON498_003115"
     CDS             427..>578
                     /locus_tag="ON498_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941287.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DAJSVD010000306          559 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJSVD010000306 DAJSVD010000000
VERSION     DAJSVD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.322"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..410
                     /locus_tag="ON498_003116"
     CDS             <1..410
                     /locus_tag="ON498_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3941288.1"
                     /translation="RNIFDKLPKKVSSDVKDELKSIFKASELELTRERKEHFLEKYGC
                     DSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLERLNEEIRRRERVIRIFPNI
                     NSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          176 a           99 c          120 g          164 t
ORIGIN      
        1 tgagaaatat ttttgataag ctgccaaaga aagtttcttc tgatgtaaaa gacgagttga
       61 agagtatctt taaagcctct gaacttgaat tgacacgtga aagaaaagag cacttcttgg
      121 aaaaatatgg ttgtgattca aaattaagtg ctgcttgcga cattttagaa aacggctttg
      181 aagatgctat tcaaattttg tcttttcctg aaaatatccg ccgaaggatt cgtacaacca
      241 atgtgttgga acggttgaac gaagagattc gccggagaga acgagtgatt cggatttttc
      301 caaatatcaa ttccatcact cggataattg gaacactttt gatggaaaaa gacactgagt
      361 ggctggcttc tccacgaaaa tatttagaat ttaattcgaa taacatttag caccatgaac
      421 ctgtgcgagt ctcttctttc tgaggagaga catcaagagt cgctgcgcta ctgctcctga
      481 cctctctcct cagaaagaaa ggcatggcag ttaggttcac aacgcaaaac attgtgcttt
      541 tgatgaggtt gaactttta
//
LOCUS       DAJSVD010000307          558 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJSVD010000307 DAJSVD010000000
VERSION     DAJSVD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.323"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>558
                     /locus_tag="ON498_003117"
     CDS             <1..>558
                     /locus_tag="ON498_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3941289.1"
                     /translation="LEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVG
                     RAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDY
                     SQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFES
                     VPKNLFKSITFDCGKEFSNWKQISN"
BASE COUNT          192 a           95 c          111 g          160 t
ORIGIN      
        1 ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga cgtccgcttg
       61 ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga tggacacccg
      121 atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc atttatcgta
      181 tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag cgtaaaccga
      241 atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat gaacgtgaaa
      301 aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact atcgtaggtc
      361 tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt atcatcacat
      421 tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa tggtttgaat
      481 ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa ttttcaaatt
      541 ggaaacagat cagtaatg
//
LOCUS       DAJSVD010000308          549 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJSVD010000308 DAJSVD010000000
VERSION     DAJSVD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.324"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>549
                     /locus_tag="ON498_003118"
     CDS             <1..>549
                     /locus_tag="ON498_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCR3941290.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQR"
BASE COUNT          182 a          108 c           96 g          163 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgta
//
LOCUS       DAJSVD010000309          527 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJSVD010000309 DAJSVD010000000
VERSION     DAJSVD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.325"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(45..347)
                     /locus_tag="ON498_003119"
     CDS             complement(45..347)
                     /locus_tag="ON498_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941291.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          181 a           92 c           74 g          180 t
ORIGIN      
        1 ggtgataaaa tccgttccgg gctacctttt ttaggtagcc tactttaatc tttatacctt
       61 tctggatcaa cgaaagtata cttcaaatag tcataacgcc gatgatcgct ccgtgcgtct
      121 ggcacatcag tcacgatatc aaacaaaaaa tacacatctt tcttcattct ggtttttgtt
      181 gccggaattt taaagtagtt cttattagag taatagagat tgattaataa gctatcttcg
      241 attgctaaaa agaaaacttc tgaatcccac accttataaa aatctttgac aaatctattc
      301 gaaggatcaa atttaaacca taattgtgtt tttccttcca tcaacataat gttcacctca
      361 aaaagagtat acgaactaat gttctttttg taaagtagga atatcattga taatatggat
      421 ggaacatata tattaaaaag tgttcttttt gaatgctatt ttgaatgcca ttttcattca
      481 tttaacgaaa atctgcgaaa ataaaaaatg ctcaaaccct tataaaa
//
LOCUS       DAJSVD010000310          526 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJSVD010000310 DAJSVD010000000
VERSION     DAJSVD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.326"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..354
                     /locus_tag="ON498_003120"
     CDS             <1..354
                     /locus_tag="ON498_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3941292.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="ON498_003121"
     CDS             355..>526
                     /locus_tag="ON498_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941293.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAJSVD010000311          516 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJSVD010000311 DAJSVD010000000
VERSION     DAJSVD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.327"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            187..>516
                     /locus_tag="ON498_003122"
     CDS             187..>516
                     /locus_tag="ON498_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCR3941294.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYE"
BASE COUNT          197 a           60 c           87 g          172 t
ORIGIN      
        1 tatagctgat ttggattcaa ttcatattta tttataaatc aaaaatacag agaatagtat
       61 ataaatgtcc tttcctattc ttaaaatgat gtttgtactt acttaaatta ggtataatta
      121 gtatatgttt tgtgagaaat ttagacttac aataagcagt gaatttataa tcaacgtata
      181 ggagacatgg atgaaatgaa actaattggt atagatttag acggtactct tttgaattct
      241 gaccagagca ttagtcaaaa aaatgcaaga acaatgaaac atttaccgga agactgtttt
      301 ccattcattt gttctggaag agaagtagaa gacattaata atattctcaa aaaatctggt
      361 ttaaggatac cagcagtagg gttaaatggt tctgttggat ttgatggaga aaaaaagata
      421 tttgagtctt ccttcgataa aaatgatgta aaaaaaattg attctgtcct atcgaaattt
      481 ccaaccaaaa tatatacaaa tctcggaagt tatgaa
//
LOCUS       DAJSVD010000312          515 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJSVD010000312 DAJSVD010000000
VERSION     DAJSVD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.328"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(34..107)
                     /locus_tag="ON498_003123"
     tRNA            complement(34..107)
                     /locus_tag="ON498_003123"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71..73),aa:Ile,seq:cat)
BASE COUNT          174 a           83 c           98 g          160 t
ORIGIN      
        1 tctaacacag aacgattcaa ttggctacat taatgtaccc tgtaggactc gaacctacga
       61 ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtagtgg ttgttgccac
      121 ataaagccat aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta tcgcatatct
      181 tggagtgtga ctatttatga gtgatagtga agatatgcgt ctccatatat gtatggttga
      241 gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga
      301 gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa
      361 acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac
      421 ttttgttcgt acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt
      481 atggtttaag catgttcctt caaataagtt tttta
//
LOCUS       DAJSVD010000313          513 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJSVD010000313 DAJSVD010000000
VERSION     DAJSVD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.329"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            251..>513
                     /locus_tag="ON498_003124"
     CDS             251..>513
                     /locus_tag="ON498_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941295.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEV"
BASE COUNT          217 a           56 c           94 g          146 t
ORIGIN      
        1 acgatgtaaa caagattttt ttcagaagaa aaaacgataa aagttgtgat aaacgattta
       61 aaaaatagga gaaaatatat caagcgcatg gtatgaaacg cacatttaaa aagattcgac
      121 taactaaaaa aataagttta caaagcattg ttgtgtatta caagtgatct atacactgaa
      181 aaatgataga gaaaatctga tacgtccgca acaatggaca attgaaaatg aaagtgagga
      241 aattggaaga atggctgatg aaaataaagt tgttccgctt gaaacactag tgataaataa
      301 ggaagagttt gagcaacttt ttcttgattt ttttgttcaa tatgacagga tttatgattt
      361 tagttcgtca ctagatttat ttgaattaga ggcagaaata atagaaaaca gtcgagagga
      421 gaatggtaaa atcatcattt cagtagaaaa attcaatgaa ttattctcag aaacatttga
      481 aatagataca gattattatc aaccacaaga agt
//
LOCUS       DAJSVD010000314          498 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJSVD010000314 DAJSVD010000000
VERSION     DAJSVD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.330"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>498
                     /locus_tag="ON498_003125"
     CDS             <1..>498
                     /locus_tag="ON498_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3941296.1"
                     /translation="DRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHP
                     WEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTL
                     IIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQL
                     YDEVM"
BASE COUNT          177 a           62 c           89 g          170 t
ORIGIN      
        1 agatagatta ctttatctat cttctacatt tttgaagaga aaagaaattc aatatattaa
       61 gtctaaagtg acgcataata aaaaaatttc ttatcaatac catgtaggac agtatttacc
      121 cgactggcat ccttgggaag attatcgcaa tttttttgtt tcaaacaaaa aaactaatgg
      181 gtgtagagaa atattcgcta ttgaattacc ttggattata gatacatttg ggaaagttaa
      241 atcgtttact gtaatgaccc agaaattatc caatcttgat attgattatt cggacagttt
      301 cactcttatt attgagcatg aaactggtat agttggaact ttaagtgtag atgttgtttc
      361 aagagtagct aaaagaaatt taactataat tggtgaaaag attttaatag aatgggaagg
      421 taacccggat tctctatttt catggaatga agaaacttct agtatgacag atattcaatt
      481 gtacgatgag gttatgca
//
LOCUS       DAJSVD010000315          496 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJSVD010000315 DAJSVD010000000
VERSION     DAJSVD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.331"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            46..381
                     /locus_tag="ON498_003126"
     CDS             46..381
                     /locus_tag="ON498_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941297.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          187 a           61 c           89 g          159 t
ORIGIN      
        1 acaatttcct aggaaaaata gttaacataa aaggaatgaa ttgttatgaa acaatattta
       61 aatgaatgga aagcgataga ggggtcatta gtaacgcaaa gaataaagga attacctgat
      121 tgcttagaga aggatcattt gtttcaaata cgagaaatgt tgaaaaaaga acagtttgat
      181 ccagaccaat ttttagtagt cgagtatcca actattggag tgtactgttg taaccatatc
      241 aatgatggaa aatattttat tattcaggag tatggaggac agttaactcc tttctatact
      301 acatgggaaa tgagtgaaga aggaatcaat aattttccat gtgaaagcat cgaagattca
      361 atttcacaca ccgaatgtta attgattata actataaaaa aggattgaac attatgttca
      421 atcctttttt caatgaaatg ttttccgcca tttcattgtt tggtgcgatt tctattttat
      481 tatatcaaaa taaaga
//
LOCUS       DAJSVD010000316          487 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJSVD010000316 DAJSVD010000000
VERSION     DAJSVD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.332"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
BASE COUNT          163 a           69 c           72 g          183 t
ORIGIN      
        1 attttaaatt tttacatacc tgtttatgaa aaactaaata atctgtcaaa aaaatatgat
       61 tgttcatttc taaatatgtt ttccaatttt aaaatctttt ttagcatttt tgtttccttt
      121 tcatatggtt tttcgttgtt aatagtgact aggatgatat tcctattttg accattgaca
      181 attaaatata atttttgaat aacgttccct gctattcgag ctaagaattc aagtacgcca
      241 tgacctattt agcgataact acttagagat ttctttttgt tttgggaaga caaaatgcca
      301 tgacaatagc agtagataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      361 aattcctatg tgcttaccca agtcaattca aaaattttag ttcatttata aggggatgat
      421 tttattaaat caatagtcta tctaaacaaa aaacaaacct gtgagttttt aggaattgat
      481 aacaata
//
LOCUS       DAJSVD010000317          470 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJSVD010000317 DAJSVD010000000
VERSION     DAJSVD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.333"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(114..413)
                     /locus_tag="ON498_003127"
     CDS             complement(114..413)
                     /locus_tag="ON498_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3941298.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          144 a           93 c           56 g          177 t
ORIGIN      
        1 atctgtttct ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc
       61 tgcattatga gattgactat actttttaat tcattgagat gaataactcg tttttataaa
      121 tgcccaaaag taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct
      181 attgttgata tctccatata aaacggtgat actggtttag acaattctcc cctgattgct
      241 aacggacgta aactacgttc caaattattt atgactttag gataatactg acctttttct
      301 aagcctgtct ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa
      361 acgagatcat ataattttag taccatgttt tctatttctt cacttttaga catcacgatt
      421 tcctcccaaa aatatatttc ttcccactac attctataaa caaagtatac
//
LOCUS       DAJSVD010000318          459 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJSVD010000318 DAJSVD010000000
VERSION     DAJSVD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.334"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..>459
                     /locus_tag="ON498_003128"
     CDS             <1..>459
                     /locus_tag="ON498_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3941299.1"
                     /translation="LITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYD
                     YSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGT
                     NVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTA"
BASE COUNT          176 a           67 c           80 g          136 t
ORIGIN      
        1 attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa tttctcagcc
       61 gggacatatt gttgtaaaca aagaaacagt agaggcgtat accactaaag gaatggatta
      121 ttttttatat gattattctc ctatcgcttc attggagaat aaggagcata taataaaaca
      181 cgaagcaaag attggcagtc aatgggtaaa ccttccttat aaaacaactg gtagttctat
      241 catatataga gtaactaatg agaagagcca agtaatcaca cttcctgtat atgcttattt
      301 aggaacgaat gtagaaataa atcatcaacc tgtaaaggag caggtgaatg ctaatggatt
      361 ggtacaaata agcggagtca aaggagttgc tgatattaaa gtaagcacaa gttatacagt
      421 aacgactatt atttcttatc ttgttacatt aactgcata
//
LOCUS       DAJSVD010000319          447 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJSVD010000319 DAJSVD010000000
VERSION     DAJSVD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.335"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ON498_003129"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ON498_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCR3941300.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          140 a           78 c           78 g          151 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcga
//
LOCUS       DAJSVD010000320          443 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJSVD010000320 DAJSVD010000000
VERSION     DAJSVD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.denovo.336"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            88..423
                     /locus_tag="ON498_003130"
                     /pseudo
     CDS             88..423
                     /locus_tag="ON498_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          152 a           45 c           81 g          165 t
ORIGIN      
        1 ggaagatgta gagttatttg cactgttgtg ttggtatatc tcttaatgct gtgtcttttt
       61 ggggagatta attagtggag tgattatatg agtatagatg attgtttaga agaacaaata
      121 agattatttt taaagttttt aaaagttaaa gttcaaaata actacttcac atgttatagt
      181 tatgcacacc ctgttttaaa tagtcttcct tttaattctt tagtgcaagg attgattaca
      241 aatattgttt tattagtctg tacaccaaat gaaatatttg taaaaaagat aggcagtggt
      301 atttcttcag taagattaaa agagaaagaa tatactcaaa agattattca tattcctaag
      361 cctgaaatta agcgttttga gataaaagac tattctgctt tagggctgtt ctcaattggt
      421 tactggtgat aaaaaataag aat
//
LOCUS       DAJSVD010000321        18630 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.049, whole genome shotgun sequence.
ACCESSION   DAJSVD010000321 DAJSVD010000000
VERSION     DAJSVD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.049"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(<1..451)
                     /gene="whiA"
                     /locus_tag="ON498_003131"
     CDS             complement(<1..451)
                     /gene="whiA"
                     /locus_tag="ON498_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3941301.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIF"
     gene            complement(485..1483)
                     /locus_tag="ON498_003132"
     CDS             complement(485..1483)
                     /locus_tag="ON498_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3941302.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(1480..2364)
                     /gene="rapZ"
                     /locus_tag="ON498_003133"
     CDS             complement(1480..2364)
                     /gene="rapZ"
                     /locus_tag="ON498_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3941303.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(2499..3230)
                     /locus_tag="ON498_003134"
     CDS             complement(2499..3230)
                     /locus_tag="ON498_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3941304.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(3234..4499)
                     /locus_tag="ON498_003135"
     CDS             complement(3234..4499)
                     /locus_tag="ON498_003135"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3941305.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(4762..7578)
                     /gene="uvrA"
                     /locus_tag="ON498_003136"
     CDS             complement(4762..7578)
                     /gene="uvrA"
                     /locus_tag="ON498_003136"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3941306.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(7588..9582)
                     /gene="uvrB"
                     /locus_tag="ON498_003137"
     CDS             complement(7588..9582)
                     /gene="uvrB"
                     /locus_tag="ON498_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3941307.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            9841..11343
                     /locus_tag="ON498_003138"
     CDS             9841..11343
                     /locus_tag="ON498_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3941308.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11345..12082
                     /locus_tag="ON498_003139"
     CDS             11345..12082
                     /locus_tag="ON498_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3941309.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12253..13446)
                     /locus_tag="ON498_003140"
     CDS             complement(12253..13446)
                     /locus_tag="ON498_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3941310.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(13525..13731)
                     /locus_tag="ON498_003141"
     CDS             complement(13525..13731)
                     /locus_tag="ON498_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3941311.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(13724..14866)
                     /locus_tag="ON498_003142"
     CDS             complement(13724..14866)
                     /locus_tag="ON498_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3941312.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            15378..16013
                     /locus_tag="ON498_003143"
     CDS             15378..16013
                     /locus_tag="ON498_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941313.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            16167..16244
                     /locus_tag="ON498_003144"
                     /pseudo
     CDS             16167..16244
                     /locus_tag="ON498_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            16489..16605
                     /locus_tag="ON498_003145"
     CDS             16489..16605
                     /locus_tag="ON498_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3941314.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            16621..18540
                     /gene="tet(M)"
                     /locus_tag="ON498_003146"
     CDS             16621..18540
                     /gene="tet(M)"
                     /locus_tag="ON498_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3941315.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         5395 a         3813 c         3413 g         6009 t
ORIGIN      
        1 aaaatatttc caaatgataa cgactcgttt ccggattatt gactgaacct gaggccataa
       61 aagcaccacg taagtaagaa cgcatttttt gcgaatttcc cataatctcg tccgaaacgt
      121 gtccattgaa catcatacca tccatgatat caagatccat caacacatct tttgtgcctt
      181 gcttcaaccg gacgatatag acattgttct tttttaattt cattttttta cgaacgagta
      241 gctcacttcg tacctgataa tgatctttca aaagagaata gatacgtctg gcaattgcag
      301 cattttctgt ttggacgttc aaaataaact gctggttcac aatacctaaa gagccgttca
      361 ttcgcagcaa agcagctaat tccgctttcg catgttctcg atgaacttcc aaaccagtta
      421 gttctttctt cacatctgca gcaaatgaca tcctattctc ctcctttctc caacaataaa
      481 ttgcttaata ttttgaaccg aaaactaaac ggaacaattc ttctactact ttgtctccat
      541 cgtggaagac accaccatct ttcaattcta aaaagtcggt ggaaatcact cgacatcctt
      601 catttctcaa tccgttgaag tcgtgtgcga cttgtactag atactcatca tagatctctg
      661 ggtccatata atcttcaggc actttttccg tattgaccaa tactgtatcg ataaaaggct
      721 gattcaaatg cttgtgtaaa acacgtatat gatcggcatc tgtgaaatgt tccgtttccc
      781 ctttttgggt catgatatta cagatataga cggtttcagc agttgtcttg agcattgctt
      841 cgccgatttc tggtatcact aaatttggaa ggatactggt gaataaactt ccgggtccca
      901 aaacgaccat gtcggcgtcc ataattgctg aaacgacttt tcgagccgct ttggcttcac
      961 ttttatcatt tgtgttgcag acatacacac ggtcaatggt tttacggttg atcgcgattt
     1021 tagattctcc tactgcagtc gttccgtctt taaaaacagc atgcaaggtc aaaggagttt
     1081 ctgaagaagg ataaacatgt ccatctacat gcatcatttt tgctaataat tgaatcgcct
     1141 cataagtgct tccccgcatc tcggaaactg cggcaatgat cagattaccg atcgtatgat
     1201 tggctaaaaa ctgatcttcc tttttgaaac ggtattggaa aatatcttca tagaattgtg
     1261 gcatatccga taacgccact aagacatttc tcaagtcacc aggaggagcc atagcgatcg
     1321 attctctgat tgcaccgctg cttccaccat catctgcaac agtcactact gctgtgatat
     1381 ctgctccttg atttcgtaaa cttttcagga taactggcag accagtgcca ccgccgacca
     1441 ctacgatctt tggttttctt atccggtagg ttttcatttt cacgaccggt tcaccgtctc
     1501 tttcctttta tccttgtccc gatgtgtcac attgacttta tagtttttcc ctaaagcatt
     1561 tgccaaacgt tcagtcaatg ccacagaacg gtgttgaccg cctgtacagc cgattgcaac
     1621 tgtcaagctg ctttttcctt ctttaatgta tccaggcaat acagtttcca gaagatccaa
     1681 aaatttatgg tagaatgatt gcgtttcatc aaaggacatc acataatcat agactggctg
     1741 atcttttccg gtcaatggtc ttagttccgg gatataatga gggttgggca aaaaacgcac
     1801 atccatcaca atatccgaat cgatcggcaa cccgtattta aaaccaaatg atacaaattc
     1861 gatccgaaat ccttggtctc cctttgattt gaatgactca ttgatttttt cacgtaattg
     1921 tcgtggtgtc aatgttgtcg tatcaatgat cacggaagct tgggctttca agtcgtctag
     1981 tatcgcccgt tctttacgta tcccttcagt gatcaaaccg tccatcgcca ttggatgtgc
     2041 tcgtcttgtt tctttgtagc gagccactag ttcttcgtct gaagcatcta aaaataagat
     2101 agaagtgtcg atgaagttcg tattttcgat ttcgatcagc atgttttgga tttcttcaaa
     2161 gaaggaacga gaacgcaaat ccactcctaa agccattttt gtgactttac ctgattcttt
     2221 gatcaattcc cagaattttg gaatcaatcg aggcggcata ttatcgatac aaaaatagcc
     2281 catatcttca aaactttgga tagctactgt tttccctgca ccactcatac ctgttatgat
     2341 gactaattgc aaattatctg tcatatttac gaacccctct cacattgtgc ataataaaat
     2401 tatatcatcc cgggcgtatc attaccagtt ttctttttct tttacaaaaa gatttggacc
     2461 aaatatcttt taggatattt gccccaaatc caattttttt acttttccga tgctgtatcc
     2521 aatgcctcgc ttctttcagc taatgcacgt tcgatcgttc gatccgctaa aatagattga
     2581 gcatcgatca ctgggtaatg attatcttca gcaaattcat gcatcaaaga taattctgtg
     2641 caacctagga tgactttttc acagtttagg cgctctaccg cttcctctaa gatttcgtaa
     2701 tagagctctt gattcaaata atccgattcc ttgatctcat gatagatcag gtaattgatt
     2761 ttttcttgaa gcgcagtatc aggaatcact gtttcgaaac caagattttt gacttcgcgt
     2821 tcatagattc cagctttcat acttccttct gttcctagaa tcgctactct ccctgtcgta
     2881 tgctgacgga caagttcatt tgctgcttct cttggcatat gcaagatagg aatatccgtt
     2941 gccgcttgta actcttcaaa aaagtagtgt gcggtgttac aagtcaacac aatgaagttt
     3001 ggtcgcaata aattttgttt ctcaatatca tccagcagaa acggcattgg attttcttct
     3061 gatcgatcta aaatataagc tgtacgatca ggaactgttg catgattgaa taagacataa
     3121 ttcaaatatt cttgatcttt tgtagctttc gttcggtggt tgatcaagcg aacaaaactt
     3181 tctgtcgcca tcgtgcccat tccgcctaaa atactgaaaa aattctccat gaactagcct
     3241 tctttcacat gaaaatactt tttgaatctt ggaatatagt tatggaacat atatttcatc
     3301 aacaaccagc gcttgattga tcggtctttt tcataaaata cagtagtacc gtaacgattt
     3361 tctttgatca tttgctcagc tagttttttg tcttcatttt cccgagcata ttctaaaaag
     3421 atttttttgg gcacacccat ccataatctt gctttatctg attgattcgt tccataggtc
     3481 gtttcaacaa agggtttgtt gaacactcga tcttctgtga caaaacgagc cagattcaag
     3541 ccgttcaatg tcacaaagaa actgctacga ccttgtctca aattgatttc aaataattta
     3601 tattctccat ctctaggatc atacttcata tcgaaattgg caaagcctgt atattcgatt
     3661 ttttccaaaa atgccttaat ggtttgatag attttttcgt tataatctgg catgatcaca
     3721 acgtaattac caatcgatgc tggtgtagga tcttctaata gtggatgacc aaggcacatc
     3781 atccgaactt gatgatcttc atctacatac gcatttagca cacgcatatt actatcatca
     3841 cccgggataa aatcttgtac aatcatttca cttttataac cggcttcgta tatccggcct
     3901 aaaattaaat cgaactcttc tctagtctct aaaataaaag ctttttttct tccttcaaat
     3961 tggacagaaa gatactccac actgtttgca ggtttcaaag caactgggaa ttcaaaaggc
     4021 aattcctgtt ccaaatgtcc atttactaac atttcttctc tgacgatcaa tgttttagga
     4081 tacggaagat catattcttc acaaacttca taaaagctta ctttattgat cagcctttca
     4141 aataaagaat aatcaatata tggacagata aaatattctg acagctcttg tttatgttgg
     4201 gagatcaatt ctgcatatcc atcaccacaa gcaatcaaaa gatatttttt actcttatca
     4261 gaatatcttt cttttgccaa acgaagcatc gtctcgataa atacagggtc tttatcaaat
     4321 cctggaataa cttctacatt gacaatttta ctgtagcggg taggggctaa ctgatccgaa
     4381 gcatatgctt ggcagatttt gccgtatgct tcattaaaag aacgtgccat accataaacg
     4441 ttcatgtcgc ttcctaataa tatagggata aattcttcat tttcaatact gttcatcatt
     4501 atctctcact tactcataat ctcttgtttt tcttttccaa ctacacaagg aatatcatac
     4561 cataaagaaa aaagcaatca aaggaaagtc ctccgattgc tcaattcttt tttgaacgga
     4621 ttgaacttat aggaagtttt tatctatacc aatcagtgta gaaagaacta ccatgtaatg
     4681 attctagaac actgatatta ataaaacaag caaaaattca tttttttatg tttttttaac
     4741 tatctcagct atctatcctc tttataccat tatttttttc aaatagtgac ctgtataact
     4801 ttcttttacg ttaatgattt cttctggtgt tccagtcgca aggatcgttc ctccaccttc
     4861 tccaccttct ggtcctaaat caatgatata atctgctgtt ttgattacat ctagattgtg
     4921 ttcaatcact aaaactgtgt taccagcatc tactaatctt tgtaatacat gcaacaagcg
     4981 ggcgatgtca tcgctatgaa gtcctgtcgt tggttcatct agtatataga aattctttcc
     5041 attagagatt ttgtgcaatt cactggcaag tttcatccgt tgtgcctcac caccggacaa
     5101 tgtcgttgct ggttgcccca tcgtcacata acctaagcca acatcaacaa tcgtttgcag
     5161 tttgcgatga atctttggaa tgtgcttgaa gaattctaca gcatcctcta ctgtcatttc
     5221 caaaatatca gcaatgcttt ttcctttgta atgcacttct aatgtttcag aattatatcg
     5281 tttaccatga catacttcac aaggaacata gacatcaggc aaaaagtgca tttcgatctt
     5341 aataattcca tccccgcgac aagcttcaca acgaccgcct tttacgttga aactaaaacg
     5401 ccctttctta taaccccgca tttttgcctc gttcgtttga gcaaataacc cacggatatc
     5461 atcaaataca cttgtataag tcgctggatt acttctcggc gtccggccga ttgggctttg
     5521 atcgatatcg atgatctttt cgatactttc gtagccggaa attgtcttga atttacctgg
     5581 cttagcagaa ttcttattta atttttgcgc taacgatttc tttaagatac tattgaccaa
     5641 tgtactcttt ccagaaccag acacacctgt gactgctaca aattctccta atggaaattc
     5701 aacactaata tttttcagat tgttttctga agcaccagtg atcttgattg ctttcccatt
     5761 acctttacgg cgttcttttg gtacagggat tgattttttc cctgaaagat atttccctgt
     5821 caaagaattt tcatcattag ctacttcttc aggagtgcct gcagccacga tttgtccgcc
     5881 ttgttctccg gccccaggtc cgacgtcaat caaataatct gctgcccaca tcgtatcttc
     5941 atcatgttcg actacaacga gggtgttgcc aagatcacgc attttcttta atgagccaat
     6001 cagtcgatcg ttgtcgcgtt gatgcaatcc gatcgatggt tcatctaaaa tatacaaaac
     6061 tccagaaaga ttcgatccaa tttgtgtagc caatctgatt cgctgcgctt ctcccccaga
     6121 taatgttccg gaagcgcgac tcaaagttag atagtctagt cccacatttt ccaagaatga
     6181 aagtctgtca tttacttctt ttaaaatcgg tttggcaatt actttctctt gttcagataa
     6241 cgttaagttt gagaagaaat ctactgcttt ttgaatagac aactcatttg tttgcccgat
     6301 attcgttcca ttgatttgaa cagatagagc ttggggattc aatcgataac ctttacatgt
     6361 ctgacaagtt aattctgtca tataaagacg catttgctca cgcgtgaaat cactgttcgt
     6421 atcatgataa cgacgctcga tattcttcaa taccccttcg aaagtcgttt ctacatcgcg
     6481 aacgccacca aaatcatttt cataatggaa atggaaaaga tcatctgtcc cattcaagat
     6541 gatctcctga tgctcttccg gtaattcttc gaatggtgta tctaaatcga taccgaaact
     6601 ttcacaagcc tgcgctaaca tttgaggata atactgagag ctgattggat tccatggaac
     6661 gatagcacct tgactaagtg tcttcgttct atctggaatc actaaatctt gatcgacttc
     6721 taatttgaca cctaaaccat cacagtctgg acaagctcca aaaggcgcat tgaatgagaa
     6781 cagtcttggt tctagttcgc caacagtaaa tccgcaataa ggacaggcat agtgttcgct
     6841 aaacaacatt tcttcttcac cgatcacgtc aaccagagca tagccgtctg ctaatcgcag
     6901 ggccgcctct aaagaatcaa ataggcgaga acgaatccct tctttcacaa tgatccggtc
     6961 gatgacgatc gcgatatcat gttttttgtt tttctccagc tcaggcactt cacttaaatc
     7021 atagatctca ccatcgactc gaacgcggac gtatccctct ttttggattc gttcgaatat
     7081 ttttttatgt tgtccttttt tctttaccac gatcggtgcc aacacttgga tctttgtgcg
     7141 ctcaggcaaa gccattactt gatctgccat ttgatctact gattgactgg taatctcgat
     7201 atgatcgttg ggacaaatcg gatgtcctac acgtgcaaac aacaagcgta aatagtcatt
     7261 gatttccgtc acagtaccta ctgtcgaccg aggattttta cttgttgttt tctgatcaat
     7321 agaaatcgcc ggactcaatc cgtcgatgct atctacgtcc ggcttatcca tttgtcctaa
     7381 aaactgacga gcataagcag aaaggctctc tacgtatctc cgctgccctt cagcgtacag
     7441 cgtatcaaat gctagggaac tttttccaga accggacaaa cctgtaacga ccacgaattt
     7501 atctcgtgga atcgttacat ctatattttt caagttatgg gcacgtgcac catgaatcgt
     7561 aattttatcg ttagccatgt tgtcctccta ctttgaagct tttaattcta aaatcatatc
     7621 tcgcacattc gcggctgttt caaaatccaa agcttttgcc gcatctttca tttctttttc
     7681 taatttcatc aataacgtgt ctttttcttc cttcgtcatt tcttcgtacg agacttgtac
     7741 cgcttctttt gtatcatctt ccgattcttt cgtaatagaa atcaaatctc ggatttcttt
     7801 aatgatcgtt tttggaacga tcccatgttc ttcattgtac ttctgctgaa tcgtacgacg
     7861 tcttgacgtt tcatccatcg ctagacgcat agaatctgtt actttgtctg cgtacatgat
     7921 tactttacct tcttcattcc gagcagctcg tccaatcgtt tggactaagg aacgttcact
     7981 acgtaaaaag ccttctttat ccgcatccaa gatagcaaca agggaaactt caggtacgtc
     8041 taatccttca cgaagcaaat tgattccaac taacacatca aattcaccta atcgaagatc
     8101 tcgaatgatt tctgttcgtt ccagcgtttt gatatcacta tgcaaatatt tgactttgat
     8161 ccctaattct ttgaaataat ctgtgaggtc ttctgccatt tttttcgtca atgtcgttac
     8221 aaacacccgc tggtcttttt cgactcgttc atttatttcg cctactagat catcgatttg
     8281 tcccatgatt ggacgaactt cgatcactgg gtcaagcagc cctgtcggac ggatgatttg
     8341 ttggataacg gtatctgttt gttcatgttc atatggtcct ggcgtcgcag agacataaat
     8401 gatctgattg acatgttttt caaactcttc taaccgaagt ggccgattgt ctaaagcaga
     8461 tggcaaacgg aaaccgtaat ctactagcat ctgttttctc gctcggtcac cattatacat
     8521 tcctctgatt tgaggcatgg ttacgtgcga ttcgtccgcc acgatcaaaa aatcttcagg
     8581 aaagaaatcc aatagcgtat aaggcggttc accttcttta cgtccgtcca tatgccgaga
     8641 ataattttca attcccgacg tgtagcccat ttctaacatc atttcaatat cgtaattggt
     8701 tcgctgttct aaacgttgag cttccagaag tttgttttca tttcgcaaga cagttaaacg
     8761 ctgttctaat tcctctttga tattcgctac ggcatgctcc atatgttcat cattcgtcac
     8821 aaagtgagtc gccgggaaga ttgctacgtg ttccgtttct cctaaaactt cacctgtcag
     8881 tgcattgact tctcggatac gttcgacctc atctccaaaa aactccacac gtaaggcacg
     8941 ttcatctctt gaagccggaa aaatctccac gacatcaccg cgcactcgaa aacgaccgcg
     9001 ctgaaaatct atatcatttc tttcgaattg gatactgact aagtcacgaa tcaactgatt
     9061 gcgatctagt tctgctccct gtcgaatcga aactacttgt ttttggtact caaaaggcga
     9121 gcctaatcca aaaatacatg aaacggatgc aatgacgatc acatcatttc tttctaataa
     9181 tgaactagta gccgaatgtc gtaatttgtc gatctcatca tttacacttg aatctttttc
     9241 gatatacgta tcgcttgaag gcacataagc ttcaggctga taataatcat aataagaaac
     9301 aaaatattca acagcattgt tagggaaaaa ttccttgaat tccccgtaca gctgaccagc
     9361 caatgttttg ttatgggcaa taatcagtgt tggtttattc actttctcaa taagattgga
     9421 aatcgtatat gttttccctg ttccagtcgc acctaataag atttgtgctt ttttcccgcc
     9481 gactacgcca tccactaatt ggttaatcgc ttcaggctga tcaccagccg gttggtattt
     9541 cgagacaagc tcaaagtgtc gggaggtatc tctctcaatc attcttcgtc ctccttttca
     9601 ttcattcctt ttcattttac gaccaataaa aagaaaagga aagcaaaggc attccttact
     9661 aaaaaattat accacaccga acatactttc gccacttttt tatcttatta tacatgttat
     9721 gttttataac ataaaatgtt agaaactcgc atttttttca ttttttcgag cttacagtct
     9781 tgtctgattc agcacttttc gcctataata ataaaatatt ggaaatttag gaggttgatt
     9841 atgaaaagaa agcatttatt tcactcattt gttttcttaa tgacaggact tgtgacactt
     9901 gcgtttggtc atgttgcaca agctgacaac actacaacgg cagagtcaag cacaagcgag
     9961 acaatgcaaa aaatagagcc caaaaaagac gtatacgtaa ttgccagtga ctctgctttt
    10021 gctccatttg aatttcaaaa cgcaaagggc gagtacgaag gcatcgatgt aaaattgatg
    10081 gaaaacatcg ccaaactgca aggatttaac atccgaatgg actaccgtgg tttctctgct
    10141 gctttgcaag ctttggaatc aggacaagca gacggaatga ttgccggaat gactgtaaca
    10201 gatgaacgaa agaaaagcta tgatttttct gattcttact ttgatagcgg gatccaatta
    10261 gctgtcaaaa aaggagatac ttcgatcaag tcttatgaag atttgaaagg aaaaacagtc
    10321 ggagcaaaag ttggtaccga aagtgctgaa tttttaagcg accatgaatc tgaatatggc
    10381 tattctatca aatattttga tgctgctgat caattgtacg acgggttaaa agttggcgca
    10441 atcgatgcaa tgatggatga ttatccggta atcggttatg cgatcaaaca aggacaagaa
    10501 ttggaaacac cgatcaaaaa agaatctggc ggaaaatatg catttgccgt taaaaaagga
    10561 caaaatcctg agcttcttaa tatgttcaat gaaggattga aagaattaaa aagaacaggc
    10621 gaatacgata agattattaa agaatatgta gcggatggtt cagaaacttc cagtgagtct
    10681 tccactgctg atgaatcaac atttcttgga cttatccaaa acaactatca agcattgttg
    10741 aaaggacttt ggagaacaat tctcctaact ttgatctcct ttgctttggc tatagtaatc
    10801 ggtgtgattt ttggattatt cagtgtagca ccaatacgcg ctcttcgcac aattgctagt
    10861 atttatgtcg atatcatccg aggaattcca atgatggttc ttgcgttctt tattttcttt
    10921 gggttgcctg gtatcgttgg atttacgatt cctgacttcc ttgctggtgt tatcaccttg
    10981 actcttaacg caagtgctta tatcgcagag attgttcgag gcgggatcaa cgccgttcct
    11041 gtcggacaaa tggaagcttc cagaagttta ggtctttctt ataatcggac aatgcaaaaa
    11101 atcattttgc cgcaagcgat acgcatcatg atcccatcat tcgtcaatca gttcgttatc
    11161 tcattaaaag acacaacgat catttccgca atcggtgtag tcgaattgct gcaaactggt
    11221 aagatcattg ttgcacgtac aacacaaagt acgtatgttt acttgatcat tgcgatcatg
    11281 tacttgattt tgatcacagc attgacaaaa ttagcaaaag ttttagaaaa gaaggtgaag
    11341 taagatggca gaaaaaatct tagttgagca tttagtcaaa aaatacggcg ataacacagt
    11401 attaaacgac atcaatgtat ccatcaacga aggagatgtc gtctgtatca ttggcccttc
    11461 aggttcagga aaaagtacat tcttgcgttg cttgaatcgt ttagaagaac cttctgacgg
    11521 tgaaattatt attgacggcg cccacttgat ggataaaagc acagatatca acttggtgag
    11581 acaacatatc ggaatggtat tccaacactt taacctattc cctcatcttt ctattttgga
    11641 aaacatcatt ttagcaccga tggacctgaa gaaagaatct agagaagaag cagagaaaaa
    11701 agcaatcaaa ttactgcaga cagttggtct agaagataaa aaagatatgt atcctgaaat
    11761 gctttctggt ggacaaaaac agcgtgtggc tattgctcgt gctctagcga tgaaccctga
    11821 catcatgctt tttgatgagc ctacttctgc actcgatcct gaaatggtcg gtgatgtctt
    11881 gaacgtcatg aaaaagttag cagaacaagg tatgacgatg gttatcgtta cgcacgaaat
    11941 ggggttcgct aaagaagtgg ccaatcgcgt catgttcatt gacgacggaa actttttaga
    12001 agatggaacg cctcaacaaa tctttgaaaa tccgcaaaat gaacggacaa aagatttctt
    12061 gaataaagta ttaaatattt aaaataaatt atatatgtct ttttgactat ctttttatat
    12121 cttcttaaat gtccgcaaag ccttatttta taggctctac gggcattttg cttttgtggt
    12181 aaacctcaca tatctaggtc tatcttctta tattttcact atcaagcgtg gttaaaatcg
    12241 tggtaaatac ttctatgcaa ttagacgctg aacctctgat tttgcggtat ctatggacgc
    12301 atgagcatac cagttcattg tgatactgat atttgaatgc cccatgatat actgtaaatc
    12361 ttttgggttc atgttcttgc ttgccagtct tgtgcagaat gtatggcgta gcgtatgcgg
    12421 tgtgatatgt ggcaatggat tgtccttgtg gtgcttgttg tatttcttta ccatacggat
    12481 aaataaggcg ttataatcaa tcgcaacttt gggcttgccc ttatgattga caaataggaa
    12541 gttgccccgt ccgtctatca caaatggttc tgcctttgga cgttttttca taacctgttg
    12601 aaatgcctgt atcgtttctt tgcttaatgg tacttgcctt attccgcttt ttgttttagg
    12661 cgtttcaata taatagccct gttccttgct ctttagtaac tggtggtcga taatcacaac
    12721 ttcattcttg aaatcaatgt cggctactgt cagtccgcac agttccgaaa tacgaagtcc
    12781 tgtctttaac agtatcagca catcatcata atgcttgtga tacacattgt ccgtcttgat
    12841 gaatgagagt aaggcttgtt cctgttcctc tgtcaatgcg actttctttt tggtatcatt
    12901 ttctaggact tcactcaact tgaaatcaaa gggatttttc ctcacataat catcttgaat
    12961 ggctatgtag aatgacgctt ttaacgagcg tttatggttg ttaatggtat tgtaggaaaa
    13021 gcctttgtct ttcatgcgta acgcccattc cttagcgtca gagggtttga ttgtatcaat
    13081 gctcctagca cctaacttgt cctctttcaa taaccgcatg agttgttccc gttgtttctg
    13141 tgtgcttttc ttcacatttg ccctctgtgc gttctgtttg gcataaagtt ggcaaagcgt
    13201 cattttcttg ccagtgctgt cgataccgtc ctcaatatcc cgtcttatct gctgttccag
    13261 ttctcgaagt gagggttttt cccgttttcc ctttggtgtc gggtctgtgg gtgtcaatct
    13321 ccaagcatac acatattttg tgtttccaaa tgcgtctaca tatttatata agtattttct
    13381 gtctgttcgt tggctctctc cagtatgcag gatacgacct ttgttatccc gtcttttttc
    13441 tttcatggtg tctgctcctt tccttgttgg aaagagcctt gatatgactt gtgttcatca
    13501 taacacatac aaggctcatt tgcattagat agcgtccaat ttatcaatga cctgttcaaa
    13561 ctgtcggcgt ttaatctgta tgcggttgcc attcataatg agccagccag cgtccttgtt
    13621 ttcctctgcc aatcgtctta acttgttttc tccgatacgg aaatactttg acgcttcttc
    13681 aatggtaagg gtgtattttt cccatacggg aatatcctgt gcattactca taaaacaccc
    13741 cctcactttc gattacgtct gctatgtccg tacacatctg ttccacaagc atttcctgct
    13801 tttcagagag gtgggtacat tcatcaatca ttttcatgta gtttgagcct gtcagcctgt
    13861 cgatttcctc tatcatcacg attgacggca tgacctgtct ttccagccat tgcagagtgc
    13921 gttccatatt gacaggtacg gggttcattt caaaaggtaa actgtcatgg gaaatgaata
    13981 tctcccacag cggatagtcg ggaaattgca caaaatcagt aataaggtaa aacaccagcc
    14041 catgagggtt atatgtcaat aacaattcct caactgcctg tactgctttt gtaccacgca
    14101 gacgaatttc aaaacgattg ataatatccg tatgtttctt tttggttgcc tgttcctttt
    14161 gtttttcata caggcagaag tattttgtgc ttgcctttga accgatataa agggtgcttg
    14221 ctaaattacg gttttttccg cttagttttc caccctgcac attttcataa tttttacagc
    14281 gacaatccgc accgccattt ctgtattttt cagacaaaac gggaatatcc aacagaccac
    14341 acatatcatt gattgccaag tcaaaacgtc ggataacacc gtcacagtca agacagcggt
    14401 ttaaaaatga ataccaatct ctgttctgtg cctgcaagac atattccatg ttgcgtgagc
    14461 ctgccccttt taattccaag aatacgccca tgtgttcgtc atcagaagcc atgaccttga
    14521 tttctccgta ggcgtaatgc tctttatagc cataataccc atagtcataa tggataaaat
    14581 aatctgcttt catgccaagc acctttcgga taatttccaa tgcgtcagtc gttggataac
    14641 ggacagacag ataatcaacg agcagataaa aaggcttatc gcagatataa taattcagac
    14701 aacgcaggat aatattttgt gtttccttgc tggcgttggc ttttccgttc tcaaagcggt
    14761 tgatactata tcttgataca tgggtaagac ttgcaagttc tccttgtgaa agaccgcttg
    14821 caagacgttt gttcttcatt tcaagtgcaa attctaaatt gttcattgta ttcctccaat
    14881 cccacaatag ccttgaaata cctgtaaacc tctattcaaa aagtgtgata tgcactatct
    14941 tcttaaaact tctcattact actcacaact ttgggattgt atggtttttg acttgtcttt
    15001 tgtgaaatag tgcccccctg ttagataacg ggggcacaga caaggctcgc aggctcgcca
    15061 gccgacaaag catttcttcc ccttgacaca aggtgtcatg gcggtaagcc tgagaggctt
    15121 ccgccaccgt gtcagaaata ctttgtcagc aagttctgtt ttgccttttc taacaagttc
    15181 catttcaacc gatattcata tctacggatt taatcagata ggtatttctt tttgaatgtt
    15241 tttatatttc attctattgt gcttattcga ttacaagaac atagtaattc ttatatgctt
    15301 atttgtcaat atcttttttt gcctttacaa atcatattaa gcatgataga cttataatag
    15361 cgaagatata acgagatatg gagggttatg caatgaaaat aagccattta ggaaataata
    15421 tacagaccat aagaaaattt aggggaatga aacaacagga acttgcggac aaaatcggta
    15481 tcaatatgca gagcctttcc aagattgaaa gagggttgaa ctatcctgcc tatgaaacgc
    15541 tggaaaagat aatggaagtg ctggacgtta cgcccaatga attgttgtcg ggagaatgga
    15601 agtatgtcaa tcaatctgaa aaggaagtct gtcagttttt aagagccgaa gaacgtctaa
    15661 atgcagaact gaaacatgga cactatgata acttctttga cagcgaggaa gaatggctgg
    15721 aatatgagtt agaaaagtta cgggaatata ttacagacta tatcaacggg aaaagcattg
    15781 aagcgtccga cctttaccca atcaaagaat ttatccagca tttgaagttt caaaaattat
    15841 tagaccgcta tgatgattta tacagtatgg atatgtttgg ggaaagcata gagggacata
    15901 agtataagac accttatcaa gtcgtgaaaa tgataaaccc gaactcaaaa gaagatatgg
    15961 aactgttacg agaagtatta aggaataaca attttgatga tgaggacgag taactttttt
    16021 cctcttgcga gaagatacag caaacaaaaa gcccgatcaa gagtgcaaag caccaccaat
    16081 ggtggcaaag ctcttgacag ggctttttct tttctgttgt gggcaatcaa gaggaagaaa
    16141 gggataaaat ctgatttgtg gtaaatcagg caaaggcaac gcaggtatct caatttgatt
    16201 tggtacttga aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt
    16261 attacagcta ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa
    16321 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
    16381 aaggtattct taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt
    16441 ttaggagggc ttagtttttt gtacccagtt taagaatacc tttatcatgt gattctaaag
    16501 tatccggaga atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat
    16561 aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac
    16621 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
    16681 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
    16741 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
    16801 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
    16861 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
    16921 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
    16981 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
    17041 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
    17101 tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
    17161 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
    17221 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt
    17281 gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa attttattca
    17341 tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa
    17401 aaaagacaac gtcttgcata tatacgcctt tatagtggag tactacattt acgagattcg
    17461 gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt
    17521 gaattatgta agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt
    17581 ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa
    17641 aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg
    17701 ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat
    17761 tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt
    17821 gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat
    17881 atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct
    17941 ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat
    18001 gagagctcgg tttctcttgg atacttaaat caatcgtttc aaaatgcagt tatggagggg
    18061 atacgctatg gctgtgaaca aggattgtat ggttggaatg tgacggactg taaaatctgt
    18121 tttaagtatg gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct
    18181 cctattgtat tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt
    18241 agttttaaaa tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa
    18301 tattgtgcga acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa
    18361 atccctgctc ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt
    18421 agtgtttgtt taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag
    18481 ccccgtcgtc caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag
    18541 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
    18601 taaagatttg aattaaagtg taaaggagga
//
LOCUS       DAJSVD010000322        13813 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.072, whole genome shotgun sequence.
ACCESSION   DAJSVD010000322 DAJSVD010000000
VERSION     DAJSVD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.072"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            complement(134..577)
                     /locus_tag="ON498_003147"
     CDS             complement(134..577)
                     /locus_tag="ON498_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3941316.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ON498_003148"
     CDS             complement(795..2135)
                     /locus_tag="ON498_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3941317.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ON498_003149"
     CDS             2404..3300
                     /locus_tag="ON498_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3941318.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ON498_003150"
     CDS             complement(3450..4391)
                     /locus_tag="ON498_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3941319.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ON498_003151"
     CDS             4517..5638
                     /locus_tag="ON498_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3941320.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON498_003152"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON498_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3941321.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="ON498_003153"
     CDS             7426..7992
                     /locus_tag="ON498_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3941322.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="ON498_003154"
     CDS             8203..9264
                     /locus_tag="ON498_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3941323.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="ON498_003155"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="ON498_003155"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3941324.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="ON498_003156"
     CDS             10177..12159
                     /locus_tag="ON498_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3941325.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ON498_003157"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="ON498_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3941326.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..>13813
                     /locus_tag="ON498_003158"
     CDS             12881..>13813
                     /locus_tag="ON498_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3941327.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPI"
BASE COUNT         4477 a         2500 c         2906 g         3930 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg att
//
LOCUS       DAJSVD010000323         7312 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.126, whole genome shotgun sequence.
ACCESSION   DAJSVD010000323 DAJSVD010000000
VERSION     DAJSVD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.126"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            76..1344
                     /locus_tag="ON498_003159"
     CDS             76..1344
                     /locus_tag="ON498_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3941328.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1831..2295
                     /locus_tag="ON498_003160"
     CDS             1831..2295
                     /locus_tag="ON498_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3941329.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2279..2908
                     /locus_tag="ON498_003161"
     CDS             2279..2908
                     /locus_tag="ON498_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3941330.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3262..4740
                     /gene="msr(C)"
                     /locus_tag="ON498_003162"
     CDS             3262..4740
                     /gene="msr(C)"
                     /locus_tag="ON498_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3941331.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4787..5233
                     /locus_tag="ON498_003163"
     CDS             4787..5233
                     /locus_tag="ON498_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3941332.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5432..5947)
                     /locus_tag="ON498_003164"
     CDS             complement(5432..5947)
                     /locus_tag="ON498_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3941333.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6064..7266
                     /locus_tag="ON498_003165"
     CDS             6064..7266
                     /locus_tag="ON498_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3941334.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2308 a         1260 c         1521 g         2223 t
ORIGIN      
        1 ggacattgac ttgcttttac ttttttagtt acactgttat ttaggaaaag tttcataatt
       61 caaaggagaa aaaagatgaa agcaaagaat ttattattta gcgttattct tttaggaaat
      121 ctagcagttg ccaacacaac agtgctagct gaagaatcga cagaaactgt cgcaactcag
      181 acatcgagtt cttcagaaac aggaattgaa aacgatacag aaagtacttt gcagtcagct
      241 actgtctcag aaaatacaat ttcaaccgaa gaaccaacaa ctgaaacaac agaacaaaca
      301 caaaccgaag acagtacatc agcagaagct cagaacaatc cagagaatgc agaagaaatc
      361 acgaaccaat tgacagatgg gcaaacattg acacataccc atcaagcaga tttgtctgaa
      421 ttcgaaaact atactggcta tgctttttat gctcgctcat ctgctaattc tcaacaagcg
      481 ttcattgata gtattgcctc aactgcacaa agtttggcaa gtgccaatga cttatatgca
      541 tcagtcatga ttgctcaagc catcgttgaa agtggctggg ggaacagtac tttggcttct
      601 gcccctaatt acaatctatt tgggattaaa ggaagctata atggccaatc tgttacgatg
      661 cctacttcgg aatatgtgaa tgggcaatgg attacggtga atgccgcttt tagaaaatat
      721 ccttcgtaca aagaatcatt gcaagataac gtaacggtat tgaagacgac ttcttttcag
      781 ccaggcgttt actactattc cggtgcatgg aaaagcaata cgaactctta caaagatgct
      841 acggcttggc taacaggtcg ttatgccact gcgcctaatt atggttctac attgaacaat
      901 gtaatcgaaa cctataatct gacacagtat gatacagcac caacttcaac ttctccgatg
      961 tatcgtctgt ataaccgtca tacaggagaa catttatata cattgaatgc cggagaaaaa
     1021 aattatctgc caactgtagg ctgggaatat gaagggattg cttggcaagc accaaacagc
     1081 ggacaacctg tctaccgttt atacaatcct tacagtggag accatcatta cacaatggca
     1141 caatctgaga tcaactcttt aacaaaaatc ggttggcgtt atgaaggtct aagtttttat
     1201 tctggtggtt ccaagccaat ttatcgacta tttaatccaa atgaaaaaac tggtacccat
     1261 cactatacct tgagtgctaa ggaaagagat tggctaacac cgatgggctg gagatatgaa
     1321 ggaataggat tttatggtta ttaaaaagag gtagtgaaaa agctgccctg catcaagtag
     1381 taagaacagc caaacaaaaa cagattttca tgttttttgt ttggttgaac ttattaccgc
     1441 agatgcaagt ttttgaacac cgtttactca aaaaaggttg tgacaacatc ttgtcataac
     1501 ttttttatta ctgtttaagc atactgatcc aatttctaat attcgacggg attcttgcaa
     1561 aaggcgattt cggaaatttt tggcggtgta aaattatcta ctgcagaaga gctatcttct
     1621 atagtttgat aagaatcaat aatcttggtt ttagcgttgt gctttcttta tcattttttc
     1681 cgttcattct acaaaaaatt tcttttctct atacttttct attgagagcg ttttttttat
     1741 tacacagctg attctttgac ttttttcttt ttttattcta ttattgacaa gtcaatcgtt
     1801 gacgagtcaa ttattagaaa ggaggaaatt atgtctttag aagagattag tcacctttta
     1861 tatcaaatca aattagccga tcaaaaaatc gtccagatct ttgaagaaaa tatcggtata
     1921 agtcttacta gatacgagat catgatgaga ctgaaagaag aaaatccatt gctacaaagc
     1981 agtttgcaag aaaagttgca gattgatcaa ggagcagtta ctcgtcatct gaaggtattg
     2041 gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga acaaccggga agtgtatgta
     2101 gcgctaacag aaaaagccca gaagaaaatc aagcattgtg aaatggacca taaagataca
     2161 ggttccgtgc tatccccggc tttcagcgat gtagaaatga aacaatttct aacactttta
     2221 gataaattga atacccaact ttgcaatata acaacagaaa cgaaagaggt taataataat
     2281 ggaacaaaaa aatgattttt caacaattat gactggtaga agatccgttc gcgtatatga
     2341 tgaaacatac aaaatcccac acgaagaaat gctggaaatg atcaaagaag cgacgactgc
     2401 accttcttct gtcaatatga aaccatggcg ctttgtggtc gctgaaagcg aggaagcaaa
     2461 agaaatcttg cgtcctttga tccgtttcaa tacgagacaa aatgatactt cttctgcaat
     2521 ggtcatgatt tttggcgata tgcaatgtta cgaatatggc gaagaaatct acaaccaagc
     2581 ttacgaatca gggaagatgc caaaagaagt cagagatcaa caattagctg cgatcattcc
     2641 ttattacaag agtttttcaa gagaagaaat gaacgatgta gtcaaagtcg attctagctt
     2701 agcggctatg caattcatgt tagttgcgcg tgatcatggc tatgaaacaa atccaattgg
     2761 tggattcgaa gcagatcagt tggcagaggc atttggtttg gataaagacc gttatgttcc
     2821 agttattatt ttatcaatag gaaaagcggt agaagaaggt tacgagtctg ttcgtttagc
     2881 accagaaaag atcactatgt tcgaataaaa ggttttcagt tactattcag tagctattgc
     2941 tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg tcggaaaaat gactgcatcg
     3001 atgaaattac gtttcgaact tttgaataac aactaaataa attcaaaaga catgtataaa
     3061 caacaatttc atggatggag tatgtccatt ttttcgaacg acagtagaat aggtgaaagc
     3121 tatgacagat ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg
     3181 cagttttttt gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca
     3241 tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc
     3301 agctttggca accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt
     3361 atagggatca taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt
     3421 gaactttttc ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa
     3481 ttaacctatc tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta
     3541 agactggaag aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg
     3601 atacgtcttc tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta
     3661 gatagaaaaa gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt
     3721 tttgttagtc atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa
     3781 gacggagaaa tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg
     3841 gagaaaaaga ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa
     3901 aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag
     3961 aaaaagcaac aaatcagact ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa
     4021 aaagccatcc aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca
     4081 accaagccgc agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac
     4141 agccgctatc ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg
     4201 gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt
     4261 tcaggtaaga caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct
     4321 ccgaaagtaa gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc
     4381 atcattcgtt ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg
     4441 aatcacttag ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga
     4501 gaagcgaccc gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta
     4561 gatgaaccga ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa
     4621 atatatccgg gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct
     4681 gatgaagttt atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa
     4741 atgtgctacg atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa
     4801 aaaagaagga ataattttta caacaatgat gtgttttttg atggtcttgg ggatgagcgc
     4861 gtataattta tggctgcatc aagatcttca tttatcaagt ttgttgattg gtctagtgcc
     4921 tggattcgtg gtggctttca ttttggatgt cttcatcatc ggtgtgctgg cgaaaaaaat
     4981 tgcctttaaa ctaccagtaa atccagaaag caaggtccaa atgatcttaa ctatttcatg
     5041 cttgatggtt ttaggaatgg tgacatgtat gtctttattc ggtgtgatca tggaacaagg
     5101 aatacctgat caattattct ctgcttatat gaatgcttgg aaaatgaatg tgattgccgc
     5161 tttaccgttg cagttgatca tcgttggccc gctttcaaga agtgtgctaa aagcaattca
     5221 aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc ttggcatgct ttttgtcata
     5281 gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg ataggttttg gcctgtcgtt
     5341 cggatcgctt tttcttttcc aactgtattg ctcttttatt gaagataatt tgctcttcaa
     5401 tagatatttc tagaaaggtt ctgtttatcc cttactcaaa aaactgctgc atttgttctc
     5461 ttacttgctg acaccattgg agttcctgct gacaaagcat aattctgcgc tggatcacaa
     5521 cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc attcaatttt ttttgatttt
     5581 tttgcatgat tttgttcagc tgttgttctc tagtgaatag cagtccagta gctgttgttt
     5641 tatcgagcgt cgaaaaagca tagatttttg ctagaaattc atctttttgc gtaggagcgg
     5701 gagtttcctc tttgatccat tcctctacaa gagcacgtcc ttcatcagtc aagtggtaga
     5761 tctttttttg agagtgctcg tctttgtcca aaacggtcac ataaccctgc tggtctaact
     5821 tggcaagcgt tgtatagatc tgactgtgat gggcttccca aaaaattgtg atcagctgct
     5881 tcaattcata cccagaacgt ggtttggata aaagcacgca taataatata taggctaaag
     5941 tattcataaa aatcctacct ctcgtcttat tttctttatt atatcacagt aaagtttcca
     6001 cgttttacat gtgcatattg acatgtaaaa taaatcatgt atgatgctaa tagagagaaa
     6061 gggatggaaa tattggagaa tcaaatagat atacaagaac aaacaaatga agaaacaaag
     6121 gggaaaagtt cagcgctagt tgtagcagct acctgtatga tcgcttttat gggagtgggt
     6181 ctagtagacc caatcttaaa aacgattgct gcacagttaa acgctacgcc agctgaaacg
     6241 accctgttat ttactagtta catgttagtc actggtgtgg tgatgctatt tacaggtttc
     6301 ttatctagtc ggatcggttt gaaaaaaaca ttgatgacag gattgtttat catcgttgta
     6361 ttcgctggtc ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc
     6421 tggggtgtcg ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta
     6481 gttggtaaca cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct
     6541 gttggccctc tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg
     6601 gtagcatttt tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag
     6661 aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt
     6721 cgttcagttg gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat
     6781 gcgccatttt tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg
     6841 ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg
     6901 acttatcgga caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga
     6961 gctggaactt ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa
     7021 ggaattatca atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac
     7081 gttgcttcgt ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt
     7141 gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt
     7201 gttcttatca gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa
     7261 gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DAJSVD010000324         4360 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.172, whole genome shotgun sequence.
ACCESSION   DAJSVD010000324 DAJSVD010000000
VERSION     DAJSVD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.172"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            157..1371
                     /locus_tag="ON498_003166"
     CDS             157..1371
                     /locus_tag="ON498_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3941335.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1402..1620
                     /locus_tag="ON498_003167"
                     /pseudo
     CDS             1402..1620
                     /locus_tag="ON498_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2039..2512
                     /gene="dfrF"
                     /locus_tag="ON498_003168"
     CDS             2039..2512
                     /gene="dfrF"
                     /locus_tag="ON498_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCR3941336.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2639..3295
                     /locus_tag="ON498_003169"
     CDS             2639..3295
                     /locus_tag="ON498_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3941337.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3477..4007
                     /locus_tag="ON498_003170"
     CDS             3477..4007
                     /locus_tag="ON498_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3941338.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1449 a          644 c          935 g         1332 t
ORIGIN      
        1 gtctttattt gtgtgaaaaa cgaaaaagag cacaacacat caagctttta ttaattgtct
       61 gtcttttata aatgcaagta ttaattcaaa aaaacagaag aaaaaaacaa ctacaccttc
      121 ttttatactc gtaatcgtag aaaggaggcg aaaaaaatga atacattacc tatcaataca
      181 ggagatacag cattcatgat tttatgtaca gcaatggttt gcctgatgac tcctggtcta
      241 gctttttttt acggcggatt agcacggaag aataatatac taacgatcat gtctcaatct
      301 tttctctccg ttgggataac aacgattatg tgggtatttg gtggattcgg attggccttt
      361 ggacgggata tcggcggtgt tattggaaac ccggcagatt tctttttgat gagacatgtt
      421 acaagcttcc ccaacatgtt tcatggtgca acgattccat ttttaatgtt ctttctttac
      481 caactgatgt ttatcgtgat taccgttcct ttgatgacag gagcttttgc agggagattg
      541 aatttaaaag gctatattat acttgttatt ttttggaatc tgctgattta tttccctgtt
      601 tgccactgga tatggggtgg cggatttttg gatcagatgg gatttagaga tttcgctggt
      661 ggagcagtca tccacacgac agctggattc ggctctttag catgtatcct cacacttggt
      721 caaagaaaga ttcctttagg agaaagaagt cagcatagca gtcttatggc agctgctatc
      781 ggtacaggac tgctttggtt tggctggttc ggttttaatt ccggtggtgc acttcgagca
      841 gatgaacaag cagtcaatgc atttggttca acattcatcg ctcttgcatt tgcgatgatt
      901 acttggctga tcattgcaaa agtaaaagga aatggctttg attttgtcga cgtactaaca
      961 ggatccgttg ctggattggc agccattacg ccttgtgccg gttatgtaga agcaaaaagt
     1021 gccatgctga ttgggatcat tgcgggtatc gtctgtcatg ctgctgttga ttttcgtaaa
     1081 aagaaacaat gggatgatgc acttgatgtg tggggtgttc atggcatggg cggattcaca
     1141 ggtacgattc tgatagggat ctttgcatct aacagccatt tactaacgaa tccaagcagc
     1201 tggtacttct tagggattca agtgattggt gtcatcatta ctgccgtgta tgcctatgtg
     1261 ctgacaacag tgattttaaa atcggcgatg cactttacaa cgatcactac tacaaaagaa
     1321 gaacaagaag aaggattgga ctttttcttc tttggcaaca aactcaattg aaacaaattc
     1381 aaatagaaaa gaggaagatt catgcaacaa agcaaaacga aaattcctgc aaaaaaatta
     1441 acgttctttg gctttttggc tatgaccatc tcaatggtcg tttcattata tgaatatccc
     1501 acattcgcga cctcaggctt cagcttagta tttttcttgt tattaggcgg tttgctatgg
     1561 tttatccctg tggcattgtg cgcagcagag atggcaacgg tgaaaggatg ggaaaaaggt
     1621 gtttcccaaa caaaaagata ttaagaaaca gcctatgttt acctgcttgc aaacaaaaga
     1681 atattgctac tagactaaga gatactacat aagtgtgttg gtatctcttt tttgttgccg
     1741 tgaaacagca gactatttca tttctgcttg attaccttaa aacttattag tctatggttt
     1801 gtcaagggct tgttgcgtga gaaatgctga atatcaccct ttacaaaaca taggctgata
     1861 agtaactttt caaaagcaga aaagaaatgg caatgcttat aacaatatga tataatgcag
     1921 atgaagacaa aaaaggaatt tgtcgagctg gttagttcga gataattttg agtttgttaa
     1981 gatgtggtgc acttgatgga atcacaggta cgacatcata ttatggagga tgaaatagat
     2041 gataggtttg attgttgcga ggtcaaagaa taatgttata ggcaagaatg gtaatatacc
     2101 atggaaaata aagggagaac aaaagcaatt tagagagtta acaacgggta atgtggttat
     2161 tatggggcga aagtcttatg aagaaatcgg tcatccgttg cctaatagaa tgaatattgt
     2221 tgtttccacc acaacagagt atcaaggaga taatttagtt tcagttaaat cattagaaga
     2281 tgcattatta ttggctaaag gacgagatgt atacatatct ggtggatatg gactatttaa
     2341 ggaagctttg caaatagtag ataaaatgta tatcacagaa gtagatttaa atattgaaga
     2401 tggagataca ttctttccag aatttgatat caatgatttt gaagttttga taggggaaac
     2461 acttggtgag gaagtgaaat atacgagaac attttatgta aggaaaaaat gaattgagta
     2521 aattttggat ttagggtgtt ttcataaata tattgctttt tatgcatata taaattgttg
     2581 taagacaaat tccagtttgt atggggcgat aaaatcagaa tttgagagag gattgaatat
     2641 gaataaggaa tggtctgaac ttaataaaac aatgcaggca caaataaaaa agaaagatac
     2701 ttataagagg ggtattgata ctttacttac tttacgaagt cagttaatac aaaccttagt
     2761 atctttcaaa gaagaattat gcagagaaga ttttaactca atccccttca taaacgctga
     2821 tggttatcat agtaagacga ttgcttattc tatttggcat attttccgca ttgaagatat
     2881 agttgtgcat acagtgataa atgaagatga acaagtattt tttgcaggca attatcaaga
     2941 gcgtatcaat tcacctatca ttacaacagg aaatgaactc atgaaacagc agattgcaga
     3001 cttttcaaaa cagcttaatc tggaagaatt atatttatat attttcgaag tatgggaaag
     3061 cactgagaaa atgcttgaac ggttatctta tgatgaattg aaaagaaaaa taccgaaaga
     3121 aagaaaagga tacttagaat cgttgaatgt agtaaacgac aatgaaaaag caatttggct
     3181 gattgattat tggtgtaata aagatatttg tggactaatt caaatgccgt tttcaagaca
     3241 ttggattatg cacacagaag cctgtttgcg tataaagaac aagatacatt cataggcaaa
     3301 aatttcagtt tgtagaacta aggaaattgg aatttaagga ggaataaagt atggttagtg
     3361 attttataag agcagcattt ccatggattt taatgggttt atttgtagca atcagttgtt
     3421 cccttatgag taaaaaagag aaataaattc ctgtttgtat ttataaaaga gaaaaaatga
     3481 gaaaagtagt tttatttatt gccatgagcc ttgacggata tattgcggac ggtaatggtg
     3541 gagtagcttg gctaaatggt catggaaatg acaatgaaaa catcgacact tatacagaat
     3601 ttaccaaaga tatagatact gtcttaatgg ggtggaatac ttctcatcag gttgttactg
     3661 aactttctcc gcaagaatgg gtatataata aatttacaac ttatgtatta acacataaag
     3721 agtgcaattc ttcccaacaa attcatttta caagtgaaaa tcctgtttta ttattggaac
     3781 ggctaaaaca agaagcagga aaggatattt ggatttgtgg cggagcaaat cttgttcagc
     3841 agttggtaag caaaaatatg attgataaat attacatttc tgttattcct actctgttag
     3901 gaaatggggt tcgtcttttg ggcaacatag acagggaaat aaagttaagg ctttgtaaaa
     3961 cacagactta taacggaata accgatttga tttatatgcg tagataacta gagagaaaca
     4021 gaaaagctgt taaaccgacg gagcgatccg accacgttgg cgggaaaatc cttaagattt
     4081 ttccgccatg cttgcaaggg ggcggttcgc ttcggcggtt cggcgagctt gcgagccgtt
     4141 caaagccccc gttatctaac aaggggggca caaaaaacaa gaaacactat acactaacaa
     4201 gcggaaaacg ctgtatttat aaggcaaaac cccattttta cagtagtgat ataaattgta
     4261 cctatatcac tatgaaaatt gaatagtaaa tatgagaaag acagttgatt tcaaaaaata
     4321 aggctctttg tcaataagga ctgatgagtt gtgcaaaatc
//
LOCUS       DAJSVD010000325         2993 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.200, whole genome shotgun sequence.
ACCESSION   DAJSVD010000325 DAJSVD010000000
VERSION     DAJSVD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.200"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..188
                     /locus_tag="ON498_003171"
     CDS             <1..188
                     /locus_tag="ON498_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3941339.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="ON498_003172"
     CDS             203..1072
                     /locus_tag="ON498_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3941340.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ON498_003173"
     CDS             1053..1787
                     /locus_tag="ON498_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3941341.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ON498_003174"
     CDS             1820..2728
                     /locus_tag="ON498_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCR3941342.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="ON498_003175"
     CDS             2725..2973
                     /locus_tag="ON498_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HCR3941343.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DAJSVD010000326         2898 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.205, whole genome shotgun sequence.
ACCESSION   DAJSVD010000326 DAJSVD010000000
VERSION     DAJSVD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.205"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            126..1094
                     /locus_tag="ON498_003176"
     CDS             126..1094
                     /locus_tag="ON498_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3941344.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1087..2118
                     /gene="vanA"
                     /locus_tag="ON498_003177"
     CDS             1087..2118
                     /gene="vanA"
                     /locus_tag="ON498_003177"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3941345.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2124..2732
                     /locus_tag="ON498_003178"
     CDS             2124..2732
                     /locus_tag="ON498_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR3941346.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          858 a          555 c          713 g          772 t
ORIGIN      
        1 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
       61 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      121 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      181 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      241 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      301 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      361 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      421 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      481 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      541 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      601 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      661 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      721 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      781 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      841 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
      901 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
      961 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1021 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1081 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1141 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1201 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1261 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1321 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1381 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1441 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1501 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1561 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1621 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1681 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1741 gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag gctgcagtac
     1801 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1861 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     1921 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     1981 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2041 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2101 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2161 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2221 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2281 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2341 agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2401 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2461 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2521 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2581 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2641 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2701 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2761 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2821 tatagtagat agtattgaag atgtaaggca gagcgatatt gggttctgtt gcaaagtttt
     2881 aaataaagaa taaaatcc
//
LOCUS       DAJSVD010000327         2018 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.227, whole genome shotgun sequence.
ACCESSION   DAJSVD010000327 DAJSVD010000000
VERSION     DAJSVD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.227"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            62..757
                     /gene="vanR"
                     /locus_tag="ON498_003179"
     CDS             62..757
                     /gene="vanR"
                     /locus_tag="ON498_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3941347.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            735..1889
                     /locus_tag="ON498_003180"
     CDS             735..1889
                     /locus_tag="ON498_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3941348.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          660 a          383 c          436 g          539 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccac ttatgtgaaa gggtgataac
       61 tatgagcgat aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt
      121 atacttaaaa aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga
      181 atgtatagac aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag
      241 cggccttact atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac
      301 cgggaaagat acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat
      361 aacgaagccc tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata
      421 caaaaaattc agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt
      481 cattaatgtt aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac
      541 cgagttttca atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct
      601 gctatttcat gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt
      661 gcatatccgg catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa
      721 aacggtatgg ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag
      781 aacgaaaact ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt
      841 atattcgttc aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca
      901 aatatgactt aaatcacctg gacgcgatga aattatatca atattccata cggaacaata
      961 tagatatctt tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc
     1021 tttcaaaatt cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga
     1081 acgaagataa acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca
     1141 cattaaaacg gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa
     1201 atgacgttgt tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt
     1261 atttgagcct gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc
     1321 atatcacgtt ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta
     1381 cacggtataa cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc
     1441 tggtgcagat gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta
     1501 ttcacgcccc cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta
     1561 acaacatttt gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg
     1621 cgggcctctc cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag
     1681 ataagctagc tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata
     1741 cgggtggcgc gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc
     1801 agatttacgc ggaaagcaat gataactata cgacgtttag ggtagagctt ccagcgatgc
     1861 cagacttggt tgataaaagg aggtcctaag agatgtatat aattttttag gaaaatctca
     1921 aggttatctt tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct
     1981 tacacggtag acttaatacc gtaagaacga gccgtttt
//
LOCUS       DAJSVD010000328         1504 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.248, whole genome shotgun sequence.
ACCESSION   DAJSVD010000328 DAJSVD010000000
VERSION     DAJSVD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.248"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            262..345
                     /locus_tag="ON498_003181"
                     /pseudo
     CDS             262..345
                     /locus_tag="ON498_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="ON498_003182"
     CDS             394..477
                     /locus_tag="ON498_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR3941349.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ON498_003183"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ON498_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3941350.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAJSVD010000329         1481 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.252, whole genome shotgun sequence.
ACCESSION   DAJSVD010000329 DAJSVD010000000
VERSION     DAJSVD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.252"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..1391
                     /locus_tag="ON498_003184"
     CDS             <1..1391
                     /locus_tag="ON498_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HCR3941351.1"
                     /translation="LFDSRFKTLQQNLSGKSLEALELEQEEIRRFQNCSLYPVYHGSA
                     KSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRD
                     SVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRE
                     RIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
BASE COUNT          500 a          233 c          301 g          447 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct gtccggtaaa tcattagaag
       61 cattagaact cgaacaagag gaaatcagaa gatttcagaa ttgctccttg taccctgttt
      121 atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg ataacgaata
      181 aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc ttcaaaattg
      241 aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga atcctgcatt
      301 tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa atgtatactt
      361 caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt gttattttgc
      421 aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg ccacagagag
      481 agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc aaacctcaac
      541 aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg cttctacaat
      601 attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
      661 aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta
      721 cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc
      781 cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg
      841 gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag
      901 ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt
      961 gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca gcagattttc
     1021 ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca gaattgttag
     1081 agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga gcatacaacg
     1141 atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat gaggtcattc
     1201 ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta actttcttta
     1261 caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact accggtgaac
     1321 ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat atgttcaata
     1381 aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa ataagatgaa
     1441 atatttttta ataaagattt gaattaaagt gtaaaggagg a
//
LOCUS       DAJSVD010000330         1349 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.264, whole genome shotgun sequence.
ACCESSION   DAJSVD010000330 DAJSVD010000000
VERSION     DAJSVD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.264"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            46..297
                     /locus_tag="ON498_003185"
                     /pseudo
     CDS             46..297
                     /locus_tag="ON498_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="ON498_003186"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="ON498_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR3941352.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DAJSVD010000331         1302 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.267, whole genome shotgun sequence.
ACCESSION   DAJSVD010000331 DAJSVD010000000
VERSION     DAJSVD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.267"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            260..1171
                     /locus_tag="ON498_003187"
     CDS             260..1171
                     /locus_tag="ON498_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HCR3941353.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          464 a          190 c          263 g          385 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgtggttctg ttgcaaagtt ttaaatctac tatcaaataa gg
//
LOCUS       DAJSVD010000332          819 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.301, whole genome shotgun sequence.
ACCESSION   DAJSVD010000332 DAJSVD010000000
VERSION     DAJSVD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.301"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..808
                     /gene="aph(2'')-I"
                     /locus_tag="ON498_003188"
     CDS             <1..808
                     /gene="aph(2'')-I"
                     /locus_tag="ON498_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR3941354.1"
                     /translation="GLLSFTQLYGLYLFKTKFSTNKKKGYAKEKAIYNFLNTNLETNV
                     KIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLD
                     YTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLC
                     HNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY
                     GNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          348 a           60 c          129 g          282 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatctt tttaaaacaa aatttagtac
       61 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      121 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      181 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      241 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      301 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      361 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      421 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
      481 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
      541 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
      601 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
      661 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
      721 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
      781 aatttataaa aggacttata aagattgatt atataatat
//
LOCUS       DAJSVD010000333          816 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.302, whole genome shotgun sequence.
ACCESSION   DAJSVD010000333 DAJSVD010000000
VERSION     DAJSVD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.302"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            <1..95
                     /locus_tag="ON498_003189"
     CDS             <1..95
                     /locus_tag="ON498_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3941355.1"
                     /translation="RACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            96..770
                     /gene="aac(6')-Ie"
                     /locus_tag="ON498_003190"
                     /pseudo
     CDS             96..770
                     /gene="aac(6')-Ie"
                     /locus_tag="ON498_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
BASE COUNT          336 a           64 c          147 g          269 t
ORIGIN      
        1 ctagagcttg ccatgtatat gaaaaattag gtggtaaaaa tgattataaa gatgaaatag
       61 tatatgtata tgattatgaa aaaggtgata aataaatgaa tatagttgaa aatgaaatat
      121 gtataagaac tttaatagat gatgattttc ctttgatgtt aaaatggtta actgatgaaa
      181 gagtattaga attttatgat ggtagagata aaaaatatac attagaatca ttaaaaaaac
      241 attatacaga gccttgggaa gatgaagttt ttagagtaat tattgaatat aacaatgttc
      301 ctattggata tggacaaata tataaaatgt atgatgagtt atatactgat tatcattatc
      361 caaaaactga tgagatagtc tatggtatgg atcaatttat aggagagcca aattattgga
      421 gtaaaggaat tggtacaaga tatattaaat tgatttttga atttttgaaa aaagaaagaa
      481 atgctaatgc agttatttta gaccctcata aaaataatcc aagagcaata agggcatacc
      541 aaaaatctgg ttttagaatt attgaagatt tgccagaaca tgaattacac gagggcaaaa
      601 aagaagattg ttatttaatg gaatatagat atgatgataa tgccacaaat gttaaggcaa
      661 tgaaatattt aattgagcat tactttgata atttcaaagt agatagtatt gaaataatcg
      721 gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt tttaaaagtc
      781 aagtccataa tcctgtgtaa aatactatac aatgtt
//
LOCUS       DAJSVD010000334          790 bp    DNA     linear   BCT 03-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00052101
            SAMN29549305-rid15782253.guided.303, whole genome shotgun sequence.
ACCESSION   DAJSVD010000334 DAJSVD010000000
VERSION     DAJSVD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549305
            Sequence Read Archive: SRR20032430
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001473978.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001473978.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 01:08:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,191
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,050
            CDSs (with protein)               :: 3,050
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2 (5S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 29 of 78
            Pseudo Genes (incomplete)         :: 42 of 78
            Pseudo Genes (internal stop)      :: 20 of 78
            Pseudo Genes (multiple problems)  :: 12 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549305-rid15782253.guided.303"
                     /strain="AUSMDU00052101"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2009"
     gene            74..622
                     /gene="aac(6')"
                     /locus_tag="ON498_003191"
     CDS             74..622
                     /gene="aac(6')"
                     /locus_tag="ON498_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3941356.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          280 a          124 c          178 g          208 t
ORIGIN      
        1 aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca
       61 agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca
      121 gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga
      181 agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt
      241 agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt
      301 agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca attacttaga
      361 aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca
      421 tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat
      481 ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg
      541 tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat
      601 tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa
      661 ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg
      721 aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct tagtttcgag
      781 tagtaagaaa
//
